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Isolation, Purification and Quantification of Ginsenoside F5 and F3 Isomeric Compounds from Crude Extracts of Flower Buds of Panax ginseng

College of Medical, Dalian University, Dalian 116622, China
Authors to whom correspondence should be addressed.
Academic Editor: Christopher W. K. Lam
Molecules 2016, 21(3), 315;
Received: 26 December 2015 / Revised: 24 February 2016 / Accepted: 3 March 2016 / Published: 9 March 2016
(This article belongs to the Collection Herbal Medicine Research)
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In this paper, the isolation, purification and quantification of ginsenoside F5 and F3 isomeric compounds from crude extracts of flower buds of Panax ginseng (CEFBPG) was investigated by reversed-phase high-performance liquid chromatography (RP-HPLC) for the first time. The satisfied separation at analytical scale was achieved using a Zorbax Eclipse XDB C-18 column with a ternary mobile phase of acetonitrile–water–phosphoric acid (28:71:1) at a flow rate of 1.0 mL/min within 40 min. UV detection was set at 203 nm. Ginsenoside F5 and F3 was 4.21 mg and 5.13 mg in 1 g flower buds of P. ginseng (FBPG), respectively. The preparation of ginsenoside F5 and F3 at semi-preparative scale was performed by using a Daisogel C-18 column and gradient elution system of acetonitrile–water (32:68 → 28:72) at a flow rate of 10 mL/min with a sample load of 20–30 mg, and yielded ginsenosides in purity of more than 96%. Their structures were characterized by NMR and high resolution electrospray ionization mass spectrometry (HRESIMS). All the method validations showed acceptable limits. The results indicate a new source to obtain ginsenoside F5 and F3, and show that the method developed here appears to be reliable for simultaneously preparing them from CEFBPG. View Full-Text
Keywords: ginsenoside F5; ginsenoside F3; isomer; purification; quantification ginsenoside F5; ginsenoside F3; isomer; purification; quantification

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Li, K.-K.; Xu, F.; Gong, X.-J. Isolation, Purification and Quantification of Ginsenoside F5 and F3 Isomeric Compounds from Crude Extracts of Flower Buds of Panax ginseng. Molecules 2016, 21, 315.

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