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Article

Real-Time Detection of a Self-Replicating RNA Enzyme

Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
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Author to whom correspondence should be addressed.
Academic Editor: Sabine Müller
Molecules 2016, 21(10), 1310; https://doi.org/10.3390/molecules21101310
Received: 6 September 2016 / Revised: 25 September 2016 / Accepted: 27 September 2016 / Published: 30 September 2016
(This article belongs to the Special Issue Ribozymes and RNA Catalysis)
A system was developed to detect the self-replication of an RNA enzyme in real time. The enzyme is an RNA ligase that undergoes exponential amplification at a constant temperature and can be made to operate in a ligand-dependent manner. The real-time system is based on a fluorimetric readout that directly couples the ligation event to an increase in florescence signal that can be monitored using standard instrumentation. The real-time system can also operate entirely with l-RNA, which is not susceptible to degradation by ribonucleases that are present in biological samples. The system is analogous to real-time PCR, but with the potential to detect small molecules, proteins, and other targets that can be recognized by a suitable aptamer. The ligand-dependent self-replication of RNA has potential applications in molecular diagnostics and biosensing that benefit from the rapid, precise, and real-time detection of various target molecules. View Full-Text
Keywords: aptazyme; exponential amplification; fluorescence detection; in vitro selection; RNA enzyme; RNA ligation; self-replication aptazyme; exponential amplification; fluorescence detection; in vitro selection; RNA enzyme; RNA ligation; self-replication
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MDPI and ACS Style

Olea, C.; Joyce, G.F. Real-Time Detection of a Self-Replicating RNA Enzyme. Molecules 2016, 21, 1310. https://doi.org/10.3390/molecules21101310

AMA Style

Olea C, Joyce GF. Real-Time Detection of a Self-Replicating RNA Enzyme. Molecules. 2016; 21(10):1310. https://doi.org/10.3390/molecules21101310

Chicago/Turabian Style

Olea, Charles; Joyce, Gerald F. 2016. "Real-Time Detection of a Self-Replicating RNA Enzyme" Molecules 21, no. 10: 1310. https://doi.org/10.3390/molecules21101310

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