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Molecules 2014, 19(11), 17345-17355;

Bioconversion of Rebaudioside I from Rebaudioside A

The Coca-Cola Company, Atlanta, GA 30313, USA
AMRI-Albany, Analytical Development, Albany, NY 12212, USA
PureCircle Limited, Lengkuk Teknologi, Bandar Enstek 71760, Malaysia
Libragen SA, 3 Rue des Satellites, Toulouse 31400, France
Author to whom correspondence should be addressed.
Received: 22 August 2014 / Revised: 3 October 2014 / Accepted: 23 October 2014 / Published: 28 October 2014
(This article belongs to the Section Natural Products Chemistry)
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To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I. View Full-Text
Keywords: Stevia rebaudiana; structure characterization; bioconversion; rebaudioside I; rebaudioside A Stevia rebaudiana; structure characterization; bioconversion; rebaudioside I; rebaudioside A

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Prakash, I.; Bunders, C.; Devkota, K.P.; Charan, R.D.; Ramirez, C.; Snyder, T.M.; Priedemann, C.; Markosyan, A.; Jarrin, C.; Halle, R.T. Bioconversion of Rebaudioside I from Rebaudioside A. Molecules 2014, 19, 17345-17355.

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