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Open AccessArticle

Synergistic Chondroprotective Effect of α-Tocopherol, Ascorbic Acid, and Selenium as well as Glucosamine and Chondroitin on Oxidant Induced Cell Death and Inhibition of Matrix Metalloproteinase-3—Studies in Cultured Chondrocytes

1
Institute of Human Nutrition and Food Science, Christian Albrechts University of Kiel, Hermann-Rodewald-Strasse 6, Kiel 24098, Germany
2
Bayer Consumer Care AG, Basel, Switzerland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2010, 15(1), 27-39; https://doi.org/10.3390/molecules15010027
Received: 1 December 2009 / Revised: 16 December 2009 / Accepted: 23 December 2009 / Published: 24 December 2009
(This article belongs to the Special Issue Vitamins)
Overproduction of reactive oxygen species and impaired antioxidant defence accompanied by chronic inflammatory processes may impair joint health. Pro-inflammatory cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α) stimulate the expression of metalloproteinases which degrade the extracellular matrix. Little is known regarding the potential synergistic effects of natural compounds such as α-tocopherol (α-toc), ascorbic acid (AA) and selenium (Se) on oxidant induced cell death. Furthermore studies regarding the metalloproteinase-3 inhibitory activity of glucosamine sulfate (GS) and chondroitin sulfate (CS) are scarce. Therefore we have studied the effect of α-toc (0.1–2.5 µmol/L), AA (10–50 µmol/L) and Se (1–50 nmol/L) on t-butyl hydroperoxide (t-BHP, 100–500 µmol/L)-induced cell death in SW1353 chondrocytes. Furthermore we have determined the effect of GS and CS alone (100–500 µmol/L each) and in combination on MMP3 mRNA levels and MMP3 secretion in IL-1β stimulated chondrocytes. A combination of α-toc, AA, and Se was more potent in counteracting t-BHP-induced cytotoxicity as compared to the single compounds. Similarly a combination of CS and GS was more effective in inhibiting MMP3 gene expression and secretion than the single components. The inhibition of MMP3 secretion due to GS plus CS was accompanied by a decrease in TNF-α production. Combining natural compounds such as α-toc, AA, and Se as well as GS and CS seems to be a promising strategy to combat oxidative stress and cytokine induced matrix degradation in chondrocytes. View Full-Text
Keywords: tocopherol; ascorbic acid; selenium; chondroitin; glucosamine; chondrocytes; oxidative stress; inflammation; metalloproteinases tocopherol; ascorbic acid; selenium; chondroitin; glucosamine; chondrocytes; oxidative stress; inflammation; metalloproteinases
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MDPI and ACS Style

Graeser, A.-C.; Giller, K.; Wiegand, H.; Barella, L.; Boesch Saadatmandi, C.; Rimbach, G. Synergistic Chondroprotective Effect of α-Tocopherol, Ascorbic Acid, and Selenium as well as Glucosamine and Chondroitin on Oxidant Induced Cell Death and Inhibition of Matrix Metalloproteinase-3—Studies in Cultured Chondrocytes. Molecules 2010, 15, 27-39. https://doi.org/10.3390/molecules15010027

AMA Style

Graeser A-C, Giller K, Wiegand H, Barella L, Boesch Saadatmandi C, Rimbach G. Synergistic Chondroprotective Effect of α-Tocopherol, Ascorbic Acid, and Selenium as well as Glucosamine and Chondroitin on Oxidant Induced Cell Death and Inhibition of Matrix Metalloproteinase-3—Studies in Cultured Chondrocytes. Molecules. 2010; 15(1):27-39. https://doi.org/10.3390/molecules15010027

Chicago/Turabian Style

Graeser, Anne-Christi; Giller, Katri; Wiegand, Heike; Barella, Luca; Boesch Saadatmandi, Christine; Rimbach, Gerald. 2010. "Synergistic Chondroprotective Effect of α-Tocopherol, Ascorbic Acid, and Selenium as well as Glucosamine and Chondroitin on Oxidant Induced Cell Death and Inhibition of Matrix Metalloproteinase-3—Studies in Cultured Chondrocytes" Molecules 15, no. 1: 27-39. https://doi.org/10.3390/molecules15010027

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