Advances in Analysis Methods for Metabolomics and Lipidomics

Topic Information

Dear Colleagues,

The increasing need for novel analytical approaches with higher selectivity, speed, and coverage is of the utmost importance in the fields of metabolomics and lipidomics. In terms of prognostics and diagnostics, the ability to quantify and identify different metabolites and lipids in biospecimes, such as biofluids, tissues and lysates, is crucial, while, the use of high-throughput techniques in clinical settings is also highly desirable. This Topic will be focused on the development, validation, and application of original methodologies based on hyphenated methods, such as UHPLC-MS, GC-MS, and CE-MS, aimed at the qualitative–quantitative analysis of metabolites and lipids in complex matrices such as cells, plasma, serum, urine, and tissue homogenates. Particular attention will be given to method optimization and validation as well as the identification and quantitation of the profiled metabolites and lipids.

Dr. Eduardo Sommella
Dr. Giulia Mazzoccanti
Dr. Emanuela Salviati
Topic Editors

Keywords

Participating Journals

Journal Name	Impact Factor	CiteScore	Launched Year	First Decision (median)	APC
Analytica analytica	3.6	3.7	2020	19 Days	CHF 1200	Submit
International Journal of Molecular Sciences ijms	4.9	9.0	2000	17.8 Days	CHF 2900	Submit
Metabolites metabolites	3.7	6.9	2011	16.7 Days	CHF 2700	Submit
Molecules molecules	4.6	8.6	1996	15.1 Days	CHF 2700	Submit
Separations separations	2.7	4.5	2014	16 Days	CHF 2600	Submit
Spectroscopy Journal spectroscj	-	-	2023	23.4 Days	CHF 1000	Submit
Chemosensors chemosensors	3.7	7.3	2013	19.1 Days	CHF 2000	Submit

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Published Papers (1 paper)

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Open AccessReview

A Comprehensive Review on Medium- and Long-Chain Fatty Acid-Derived Metabolites: From Energy Sources to Metabolic Signals

by Jin-Byung Park, Sungyun Cho and Sung-Joon Lee

Metabolites 2026, 16(1), 45; https://doi.org/10.3390/metabo16010045 - 4 Jan 2026

Viewed by 334

Abstract

Medium- and long-chain fatty acids (MLFAs) are increasingly recognized not only as metabolic substrates but also as precursors of diverse bioactive metabolites generated through host and microbial transformations. Recent advances in analytical chemistry and microbiome research have revealed that gut microorganisms catalyze extensive [...] Read more.

Medium- and long-chain fatty acids (MLFAs) are increasingly recognized not only as metabolic substrates but also as precursors of diverse bioactive metabolites generated through host and microbial transformations. Recent advances in analytical chemistry and microbiome research have revealed that gut microorganisms catalyze extensive modifications of dietary MLFAs—producing hydroxylated, conjugated, and keto-fatty acids with enhanced potency toward host receptors. These metabolites exhibit dual activity on classical metabolic receptors, including FFAR1/4 and PPARα/γ, as well as ectopically expressed chemosensory receptors such as olfactory receptors (ORs) and bitter taste receptors (TAS2Rs). This expanded receptor landscape establishes a previously unrecognized chemosensory–metabolic axis that integrates dietary signals, microbial metabolism, and host physiology. Microbial MLFA derivatives such as 10-hydroxyoctadecenoic acid and conjugated linoleic acid regulate incretin secretion, adipogenesis, macrophage polarization, and intestinal barrier function through coordinated activation of FFARs and PPARs. Concurrently, dicarboxylic acids such as azelaic acid activate Olfr544 to modulate lipolysis, ketogenesis, GLP-1 release, and feeding behavior. TAS2Rs also sense oxidized lipids, linking lipid metabolism to immune regulation and enteroendocrine signaling. Collectively, these pathways highlight the microbiome as a metabolic transducer that converts dietary lipids into signaling molecules influencing endocrine, immune, and gut–brain circuits. Understanding the mechanisms governing MLFA bioconversion and receptor engagement provides new opportunities for therapeutic and nutritional intervention. Targeting ORs and TAS2Rs, engineering probiotics to enhance beneficial FA-derived metabolites, and developing receptor-selective synthetic analogs represent promising strategies. Future progress will require integrative approaches combining physiology, biochemistry, metabolomics, and microbial genomics to elucidate receptor specificity and host variability. Full article

(This article belongs to the Topic Advances in Analysis Methods for Metabolomics and Lipidomics)

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