Chitin and Chitosan: Preparation, Purification, Characterization, and Applications

A special issue of Polysaccharides (ISSN 2673-4176).

Deadline for manuscript submissions: 30 November 2026 | Viewed by 1601

Editor

Special Issue Information

Dear Colleagues,

Chitin and chitosan are among the most abundant and versatile natural biopolymers, garnering increasing scientific and industrial interest due to their biodegradability, biocompatibility, antimicrobial activity, and tunable physicochemical properties. Derived primarily from marine biomass, fungal sources, and insect exoskeletons, these polysaccharides are gaining prominence as sustainable alternatives to petroleum-based materials and as key components in circular bioeconomy initiatives.

This Special Issue, “Chitin and Chitosan: Preparation, Purification, Characterization, and Applications”, aims to highlight recent advances in the extraction, purification, structural characterization, functional modification, and emerging applications of chitin- and chitosan-based materials. We welcome contributions focusing on green and innovative processing technologies, including enzymatic extraction, deep eutectic solvent systems, and environmentally friendly purification strategies. Studies exploring advanced analytical and characterization techniques, such as spectroscopic, thermal, rheological, and morphological assessments, are also encouraged.

We seek research and review articles addressing the expanding application landscape of chitin and chitosan, including biomedical engineering, drug delivery, tissue scaffolding, food packaging, agriculture, environmental remediation, nanotechnology, and composite material development. Interdisciplinary contributions that bridge fundamental science with translational or industrial implementation are particularly encouraged.

By bringing together cutting-edge research, this Special Issue aims to advance knowledge, promote sustainable biomaterial development, and stimulate innovation in chitin and chitosan science.

Dr. Azizur Rahman
Guest Editor

Manuscript Submission Information

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Keywords

  • chitin
  • chitosan
  • biopolymer extraction
  • green processing technologies
  • structural characterization
  • biomedical applications
  • environmental remediation
  • bio-based materials
  • marine biomass valorization
  • functional polysaccharides

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Published Papers (2 papers)

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Research

21 pages, 3206 KB  
Article
Induction of Salt Stress Tolerance in Strawberries Using a Chitosan–Maltodextrin System
by Judith Isabel Torres-de la Cruz, Eneida Adilene Pérez-Velasco, Aida Isabel Leal-Robles and Alonso Méndez-López
Polysaccharides 2026, 7(3), 80; https://doi.org/10.3390/polysaccharides7030080 - 3 Jul 2026
Viewed by 304
Abstract
Salinity is a major abiotic constraint limiting strawberry (Fragaria × ananassa) productivity by disrupting water relations, nutrient uptake, and photosynthetic processes. Biopolymer-based biostimulants have emerged as a sustainable strategy to enhance crop performance under stress conditions. The objective of this study [...] Read more.
Salinity is a major abiotic constraint limiting strawberry (Fragaria × ananassa) productivity by disrupting water relations, nutrient uptake, and photosynthetic processes. Biopolymer-based biostimulants have emerged as a sustainable strategy to enhance crop performance under stress conditions. The objective of this study was to evaluate the effect of a chitosan–maltodextrin (CHTMD) formulation on growth, physiological response, and fruit quality in strawberry plants under saline conditions at the Universidad Autonoma Agraria Antonio Narro in Saltillo, Mexico. A randomized complete block design with a 2 × 4 factorial arrangement was established, including two salinity levels (0 and 45 mM NaCl) and four CHTMD concentrations (0, 250, 500, and 1000 mg L−1). The application of CHTMD significantly mitigated the adverse effects of salinity and improved plant growth, biomass accumulation, gas exchange, yield, and fruit quality. Under saline conditions, 250 mg L−1 increased total fresh weight by 148.5% compared with the saline control, while root length increased by up to 58.5% under non-saline conditions. Yield was enhanced by 87.3% and 71.4% with 250 and 1000 mg L−1, respectively, whereas fruit number increased by up to 63.8% under salinity. Photosynthetic rate increased from 12.58 to 16.19 μmol CO2 m−2 s−1 and stomatal conductance from 0.235 to 0.325 mol H2O m−2 s−1. Fruit quality was also enhanced, with soluble solids increasing from 5.9 to 7.1 °Brix, vitamin C from 50.58 to 115.42 mg 100 g−1 FW, and total anthocyanins from 65.7 to 106.2 C3G 100 g−1 FW, indicating a substantial enhancement to the fruit’s nutraceutical quality, particularly at 500 mg L−1 and 1000 mg L−1. These findings demonstrate that the CHTMD system is an effective biostimulant capable of improving tolerance to salt stress by modulating key physiological and biochemical responses, as well as enhancing the functional quality of the fruit. This approach represents a promising and sustainable strategy for strawberry production in agricultural systems affected by salinity. Full article
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23 pages, 4461 KB  
Article
Bioprocessing of Pacific White Shrimp (Litopenaeus vannamei) Shells for α-Chitin Extraction via Sequential Fermentation with Bacillus haynesii and Lactobacillus delbrueckii
by Gopi Manju, Pambayan Ulagan Mahalingam, Raman Krishnamoorthi, Pradeep Kumar Sudheeran, Kalyani Dhanapal, Anbalagan Indhrapriyadarshini, Arokia Vijaya Anand Mariadoss, Juyeon Lee and Kwang-sun Kim
Polysaccharides 2026, 7(2), 61; https://doi.org/10.3390/polysaccharides7020061 - 22 May 2026
Viewed by 805
Abstract
The industrial extraction of chitin from shrimp shell waste conventionally employs corrosive chemical treatments, which pose significant environmental hazards and compromise polymer integrity. This study introduces a sustainable and highly efficient microbial biorefining strategy for the recovery of α-chitin from Litopenaeus vannamei shells, [...] Read more.
The industrial extraction of chitin from shrimp shell waste conventionally employs corrosive chemical treatments, which pose significant environmental hazards and compromise polymer integrity. This study introduces a sustainable and highly efficient microbial biorefining strategy for the recovery of α-chitin from Litopenaeus vannamei shells, utilizing a sequential fermentation framework. Two potent strains—Bacillus haynesii MGPUMGRI, known for its proteolytic capabilities, and Lactobacillus delbrueckii MGPUMGRI, which produces lactic acid—were isolated and optimized. A notable technical achievement was the purification of an approximately 40 kDa extracellular alkaline protease from B. haynesii, which demonstrated optimal activity at pH 9.0 and 37 °C. Under optimized conditions, the sequential process—emphasizing enzymatic deproteinization (72.30 ± 1.56%) followed by lactic acid-mediated demineralization (84.98 ± 1.96%)—achieved a high-purity chitin recovery of 61.33 ± 1.06%. Comprehensive characterization using SEM-EDX, FTIR, and XRD confirmed the successful preservation of the α-chitin polymorphic structure, which exhibited a fragmented fibrillar morphology and a crystallinity index (CrI) of 60.51%. These findings indicate that this dual-strain bioprocess offers a scalable and environmentally friendly alternative for the valorization of seafood waste into high-quality biogenic polymers, while minimizing the ecological impact of chitin production. Full article
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