Search Results (1,259)

Wheat (Triticum aestivum L.) is a crucial global food crop. The intensive crop farming, monoculture cultivation, and impact of climate change affect the susceptibility of wheat cultivars to biotic stresses, mainly caused by soil fungal pathogens, especially those belonging to the genus Fusarium. This situation threatens yield and grain quality through root and crown rot. While conventional chemical fungicides face resistance issues and environmental concerns, biological alternatives like seed priming with natural metabolites are gaining attention. Polyamines, including putrescine, spermidine, and spermine, are attractive priming agents influencing plant development and abiotic stress responses. Spermine in particular shows potential for in vitro antifungal activity against Fusarium. Optimising spermine concentration for seed priming is crucial to maximising protection against Fusarium infection while ensuring robust plant growth. In this research, we explored the potential of the polyamine spermine as a seed treatment to enhance wheat resilience, aiming to identify a sustainable alternative to synthetic fungicides. Our findings revealed that a six-hour seed soak in spermine solutions ranging from 0.5 to 5 mM did not delay germination or seedling growth. In fact, the 5 mM concentration significantly stimulated root weight and length. In complementary in vitro assays, we evaluated the antifungal activity of spermine (0.5–5 mM) against three Fusarium species. The results demonstrated complete inhibition of Fusarium culmorum growth at 5 mM spermine. A less significant effect on Fusarium graminearum and little to no impact on Fusarium oxysporum were found. The performed analysis revealed that the spermine had a fungistatic effect against the pathogen, retarding the mycelium growth of F. culmorum inoculated on the seed surface. A pot experiment with Bulgarian soft wheat cv. Sadovo-1 was carried out to estimate the effect of seed priming with spermine against infection with isolates of pathogenic fungus F. culmorum on plant growth and disease severity. Our results demonstrated that spermine resulted in a reduced distribution of F. culmorum and improved plant performance, as evidenced by the higher fresh weight and height of plants pre-treated with spermine. This research describes the efficacy of spermine seed priming as a novel strategy for managing Fusarium root and crown rot in wheat. Full article

In the face of growing demand for food and climate change, ensuring the stability and height of crop yields is becoming a key challenge for modern agriculture. One of the solutions supporting the sustainable development of crop production is the use of certified seed. The aim of this study was to assess the impact of using certified seed on the level and stability of yields of three cereal species: winter wheat, winter triticale and spring barley, in temperate climate conditions. Data came from surveys conducted on over 8000 farms in six agroecoregions of Poland in 2021–2023. The analysis showed significantly higher yields on farms using certified seed for all species studied. Additionally, greater yield stability (lower values of Shukla variance and Wricke ecovalence) was noted in the case of using certified seeds, especially in region IV. This indicates the positive impact of certified seeds (e.g., genetic purity, health, and vigor) on the efficiency and resilience of agricultural systems. This phenomenon is of particular importance in the context of climate change and may be an important element of risk management strategies in agriculture. Full article

Background: The typification of the name Aegilops tauschii Coss. (Poaceae: Triticeae) is revisited. Several authors cited a gathering from Iberia as the locality and Buxbaum as the collector of as the type, but no actual specimens from this collection have been located, nor is there evidence that such a gathering existed. In 1994, van Slageren designated as lectotype an illustration from Buxbaum’s Plantarum minus cognitarum centuria I (1728), which, although original material, is not the only element cited in the protologue. The protologue mentions several gatherings, some of which are represented by identifiable herbarium specimens qualifying as syntypes. Methods: This work is based on the analysis of the protologue of Aegilops tauschii and the study of specimens conserved in several herbaria. According to the International Code of Nomenclature for algae, fungi, and plants (ICN, Shenzhen Code 2018), an illustration does not hold the same nomenclatural weight as preserved specimens cited in the protologue. Therefore, van Slageren’s lectotypification does not comply with Art. 9.12 of the ICN and must be superseded. Results: The original material includes multiple elements, and a new lectotype is designated from a specimen at PRC from Azerbaijan. Full article

The Fusarium genus includes some of the most economically and ecologically impactful fungal pathogens affecting global agriculture and human health. Over the past 15 years, rapid advances in molecular biology, genomics, and diagnostic technologies have reshaped our understanding of Fusarium taxonomy, host–pathogen dynamics, mycotoxin biosynthesis, and disease management. This review synthesizes key developments in these areas, focusing on agriculturally important Fusarium species complexes such as the Fusarium oxysporum species complex (FOSC), Fusarium graminearum species complex (FGSC), and a discussion on emerging lineages such as Neocosmospora. We explore recent shifts in species delimitation, functional genomics, and the molecular architecture of pathogenicity. In addition, we examine the global burden of Fusarium-induced mycotoxins by examining their prevalence in three of the world’s most widely consumed staple crops: maize, wheat, and rice. Last, we also evaluate contemporary management strategies, including molecular diagnostics, host resistance, and integrated disease control, positioning this review as a roadmap for future research and practical solutions in Fusarium-related disease and mycotoxin management. By weaving together morphological insights and cutting-edge multi-omics tools, this review captures the transition into a new era of Fusarium research where integrated, high-resolution approaches are transforming diagnosis, classification, and management. Full article

Due to their nutritional value and sustainability, edible insect-based foods are gaining popularity in Europe. Their use is regulated by EU legislation, which defines authorised species and sets labelling requirements. Molecular tools are being developed to authenticate such products. In this study, yellow mealworm (Tenebrio molitor) larvae authorised for human consumption were fed wheat flour-based diets containing varying proportions of house cricket (Acheta domesticus) flour for 21 days. This was followed by a 48 h starvation period to assess the persistence of insect DNA in the digestive tract. Two novel, species-specific, single-copy markers were designed: ampd gene for the Acheta domesticus and MyD88 gene for the Tenebrio molitor. These were applied using qPCR and ddPCR. Both methods successfully detected cricket DNA in the guts of starved larvae. Linear regression analysis revealed a strong, statistically significant correlation between the proportion of Acheta domesticus flour in the diet and the normalised relative quantity of DNA. ddPCR proved to be more sensitive than qPCR, particularly in the detection of low DNA levels. These results suggest that the presence of DNA from undeclared insect species in edible insects may be indicative of their diet rather than contamination or adulteration. This highlights the importance of contextual interpretation in food authenticity testing. Full article

Seed priming, traditionally viewed as a method for enhancing crop resilience to abiotic stress, has evolved into a multifaceted agronomic strategy. This review synthesizes the current findings demonstrating that priming influences plant development, metabolic regulation, and yield enhancement even under optimal conditions. By covering a wide range of crops, including cereals (e.g., wheat, maize, rice, and barley) as well as vegetables and horticultural species (e.g., tomato, carrot, spinach, and lettuce), we highlight the broad applicability of priming across agricultural systems. The underlying mechanisms include hormonal modulation, altered source–sink dynamics, accelerated phenology, and epigenetic memory. Various priming techniques are discussed, including hydropriming, osmopriming, biopriming, chemopriming, and nanopriming, with attention to their physiological and molecular effects. Special focus is given to the role of seed priming in advancing climate-smart and precision agriculture. By shifting the narrative from stress mitigation to holistic crop performance optimization, seed priming emerges as a key tool for sustainable agriculture in the face of global challenges. Full article

Within the framework of organic acid alternatives to chemical herbicides, pre-emergence weed control research is scarce. Citric acid (CA) and lactic acid (LA), considered significantly less effective than pelargonic acid (PA) and acetic acid (AA) from post-emergence (foliar spraying) studies, have largely been disregarded. This in vitro study was aimed at comparing the effects of 5–20% AA, AA + essential oils, PA, CA, and LA on radicle emergence inhibition (direct spraying of seeds) and shoot emergence inhibition (application to peat) on both weeds (perennial ryegrass, green foxtail, common vetch and chicory) and crops (soft wheat, alfalfa and millet). All tested compounds demonstrated concentration-dependent and species-specific effects on shoot emergence inhibition, with CA and LA (IC₅₀ range: 3.4–19.3%) showing a comparable efficacy to PA and AA (IC₅₀ range: 3.1–35.9%). The results also showed that CA and, to a lesser extent, LA were less inhibitory to soft wheat (CA IC₅₀ = 62.5%; LA IC₅₀ = 35.9%) and alfalfa (CA IC₅₀ = 57.8%; LA IC₅₀ = 44.1%) shoot emergence. CA and LA show potential promise for pre-emergence weed control in field testing, either on a stale seedbed in pre-crop sowing or concurrently with soft wheat and alfalfa sowing. Investigating organic compound herbicidal effects on crops of interest warrants attention. Full article

Weeds represent increasingly troublesome threats to modern wheat production. Jiangsu Province, China, is one of the largest wheat-planting areas, characterized by a high level of agricultural mechanization, in the country. In 2024, we surveyed weed communities of 924 wheat lands from 308 sites in Jiangsu and compared them with historical data surveyed in 1999–2000 in this province. A total of 156 weed species belonging to 103 genera and 39 families were recorded. Compositae and Poaceae showed the highest species richness, with 26 and 23 species, respectively. Poaceae weeds accounted for 54.3% of the overall weeds in dominance values. Beckmannia syzigachne showed the highest proportion of the overall weeds in dominance values (19.2%). Compared with the historical data, weed diversity increased by 33.3% in species, and by 62.5% in families; grassy weeds such as Beckmannia syzigachne, Alopecurus japonicus, and Alopecurus myosuroides became more dominant, while several low-growing grassy weeds became less dominant. The dominance of broadleaf weeds, such as Galium spp., Vicia spp., and Veronica spp., decreased greatly, and a clear trend of weed homogenization among different areas and types of wheat lands was observed. This is the first investigation of weed community succession against the background of agricultural modernization in China. Full article

Durum wheat, the world’s second most cultivated wheat species, is a staple crop, critical for global food security, including in Ethiopia where it serves as a center of diversity. However, climate change and genetic erosion threaten its genetic resources, necessitating genomic studies to support conservation and breeding efforts. This study characterized genome-wide diversity, population structure (STRUCTURE, principal coordinate analysis (PCoA), neighbor-joining trees, analysis of molecular variance (AMOVA)), and selection signatures (F_ST, Hardy–Weinberg deviations) in Ethiopian durum wheat by analyzing 376 genotypes (148 accessions) using an Illumina Infinium 25K single nucleotide polymorphism (SNP) array. A set of 7842 high-quality SNPs enabled the assessments, comparing landraces with cultivars and breeding populations. Results revealed moderate genetic diversity (mean polymorphism information content (PIC) = 0.17; gene diversity = 0.20) and identified 26 loci under selection, associated with key traits like grain yield, stress tolerance, and disease resistance. AMOVA revealed 80.1% variation among accessions, with no significant differentiation by altitude, region, or spike density. Landraces formed distinct clusters, harboring unique alleles, while admixture suggested gene flow via informal seed exchange. The findings highlight Ethiopia’s rich durum wheat diversity, emphasizing landraces as reservoirs of adaptive alleles for breeding. This study provides genomic insights to guide conservation and the development of climate-resilient cultivars, supporting sustainable wheat production globally. Full article

False cleavers (Galium spurium L.) is a broadleaf weed species that affects wheat productivity because of its strong competition for resources. It has developed resistance to acetolactate synthase (ALS) inhibitors, such as sulfonylureas and triazolopyrimidines, which are herbicides widely used in durum wheat. Integrated weed management programs can contribute to the control of this species and delay the evolution of herbicide resistance. Thus, a two-year field experiment was conducted to evaluate the effects of sowing time, variety, and herbicides on crop yield, density, and dry weight of a false cleavers population with resistance to ALS inhibitors. In both growing seasons, a split-split-plot design was used with three replicates. The sowing date was chosen as the main plot factor, durum wheat varieties as the subplot factor, and herbicides as the sub-subplot factor. The herbicide treatments were: (1) metsulfuron-methyl/bensulfuron-methyl (4/50 g a.i. ha⁻¹), (2) aminopyralid/florasulam (9.9/4.95 g a.i. ha⁻¹), (3) pyroxsulam and florasulam/2,4-D (18.75 + 4.725/225 g a.i. ha⁻¹), (4) 2,4-D/bromoxynil (633.15/601.2 g a.i. ha⁻¹), non-treated control, and hand-weeded control for the first season, while in the second season one more herbicide treatment (halauxifen-methyl/florasulam, 5.6/5.15 g a.i. ha⁻¹) was added. Herbicide application was performed on 10 March 2021 and 28 March 2022, when the crop was at the end of tillering and the beginning of stem elongation. The results showed that the density of false cleavers was not affected by the variety or sowing time. However, its dry weight was 17.3–23.4% higher in early sowing (16 November in 2020 and 8 November 2021) than in late sowing (24 December 2020 and 2 December 2021). Among the herbicides tested, 2,4-D/bromoxynil and halauxifen-methyl/florasulam effectively controlled false cleavers, showing greater efficacy in late sowing (>88%), which ultimately led to a higher yield. In conclusion, our two-year findings demonstrate that delayed sowing as part of an integrated weed management strategy can contribute to controlling resistant populations of false cleavers to ALS-inhibiting herbicides without affecting the quantity and quality of durum wheat yield in areas with a Mediterranean climate. Full article

The review summarizes the historical and current research on perennial grain breeding in Russia within the context of growing global interest in perennial crops. N.V. Tsitsin’s pioneering work in the 1930s produced the first wheat–wheatgrass amphiploids, which demonstrated the capacity to regrow after harvest and survive for 2–3 years. Subsequent research at the Main Botanical Garden in Moscow focused on characterizing Tsitsin’s material, selecting superior germplasm, and expanding genetic diversity through new cycles of hybridization and selection. This work led to the development of a new crop species, Trititrigia, and the release of cultivar ‘Pamyati Lyubimovoy’ in 2020, designed for dual-purpose production of high-quality grain and green biomass. Intermediate wheatgrass (Thinopyrum intermedium) is native to Russia, where several forage cultivars have been released and cultivated. Two large-grain cultivars (Sova and Filin) were developed from populations provided by the Land Institute and are now grown by farmers. Perennial rye was developed through interspecific crosses between Secale cereale and S. montanum, demonstrating persistence for 2–3 years with high biomass production and grain yields of 1.5–2.0 t/ha. Hybridization between Sorghum bicolor and S. halepense resulted in two released cultivars of perennial sorghum used primarily for forage production under arid conditions. Russia’s agroclimatic diversity in agricultural production systems provides significant opportunities for perennial crop development. The broader scientific and practical implications of perennial crops in Russia extend to climate-resilient, sustainable agriculture and international cooperation in this emerging field. Full article

Biodegradation is a green and efficient method for lignin depolymerization and conversion. In order to screen potential bacterial strains for efficient lignin degradation, composts of cow dung and wheat straw were prepared, and the dynamic changes in the predicted bacterial community structure and function in different periods of the composts were investigated. Then, bacteria with an efficient lignin degradation ability were finally screened out from the compost samples. Based on the monitoring results of the physicochemical indexes of the composting process, it was found that the temperature and pH of the compost firstly increased and then decreased with the extension of time, and the water content and C/N gradually decreased. High-throughput sequencing of compost samples from the initial (DA), high-temperature (DB), and cooling (DC) periods revealed that the number of OTUs increased sharply then stabilized around 2000, and the alpha diversity of the bacterial community decreased firstly and then increased. The predominant phyla identified included Proteobacteria, Firmicutes, Chloroflexi, and Bacteroidetes, determined by the relative abundance of beta-diversity-associated species. Functional gene analysis conducted using Tax4Fun revealed that the genes were primarily categorized into Metabolism, Genetic Information Processing, Environmental Information Processing, and Cellular Processes. Based on the decolorization of aniline blue and the degradation efficiency of alkali lignin, eight bacterial strains were isolated from compost samples at the three stages. Cupriavidus sp. F1 showed the highest degradation of alkali lignin with 66.01%. Cupriavidus sp. D8 showed the highest lignin degradation potential with all three enzyme activities significantly higher than the other strains. The results provide a strategy for the lignin degradation and utilization of biomass resources. Full article

Investigations of endogenous plant circular RNAs (circRNAs) in several plant species have revealed changes in their circular RNA profiles in response to biotic and abiotic stresses. Recently, circRNAs have emerged as critical regulators of gene expression. The destructive impacts on agriculture due to plant viral infections necessitate better discernment of the involvement of plant circRNAs during viral infection. However, few such studies have been conducted hitherto. Sobemoviruses cause great economic impacts on important crops such as rice, turnip, alfalfa, and wheat. Our current study investigates the dynamics of plant circRNA profiles in the host Arabidopsis thaliana (A. thaliana) during infections with the sobemoviruses Turnip rosette virus (TRoV) and Rice yellow mottle virus (RYMV), as well as the small circular satellite RNA of the Lucerne transient streak virus (scLTSV), focusing on circRNA dysregulation in the host plants and its potential implications in triggering plant cellular defense responses. Towards this, two rounds of deep sequencing were conducted on the RNA samples obtained from infected and uninfected plants followed by the analysis of circular RNA profiles using RNA-seq and extensive bioinformatic analyses. We identified 760 circRNAs, predominantly encoded in exonic regions and enriched in the chloroplast chromosome, suggesting them as key sites for circRNA generation during viral stress. Gene ontology (GO) analysis indicated that these circRNAs are mostly associated with plant development and protein binding, potentially influencing the expression of their host genes. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed photosynthesis as the most affected pathway. Interestingly, the non-coding exogenous scLTSV specifically induced several circRNAs, some of which contain open reading frames (ORFs) capable of encoding proteins. Our biochemical assays demonstrated that transgenic expression of scLTSV in A. thaliana enhanced resistance to TRoV, suggesting a novel strategy for improving plant viral resistance. Our results highlight the complexity of circRNA dynamics in plant–virus interactions and offer novel insights into potential circRNA-based strategies for enhancing plant disease resistance by modulating the differential expression of circRNAs. Full article

Azospirillum is a well-studied genus of plant growth-promoting rhizobacteria (PGPR) and one of the most extensively researched diazotrophs. This genus can colonize rhizosphere soil and enhance plant growth and productivity by supplying essential nutrients to the host. Azospirillum–plant interactions involve multiple mechanisms, including nitrogen fixation, the production of phytohormones (auxins, cytokinins, indole acetic acid (IAA), and gibberellins), plant growth regulators, siderophore production, phosphate solubilization, and the synthesis of various bioactive molecules, such as flavonoids, hydrogen cyanide (HCN), and catalase. Thus, Azospirillum is involved in plant growth and development. The genus Azospirillum also enhances membrane activity by modifying the composition of membrane phospholipids and fatty acids, thereby ensuring membrane fluidity under water deficiency. It promotes the development of adventitious root systems, increases mineral and water uptake, mitigates environmental stressors (both biotic and abiotic), and exhibits antipathogenic activity. Biological nitrogen fixation (BNF) is the primary mechanism of Azospirillum, which is governed by structural nif genes present in all diazotrophic species. Globally, Azospirillum spp. are widely used as inoculants for commercial crop production. It is considered a non-pathogenic bacterium that can be utilized as a biofertilizer for a variety of crops, particularly cereals and grasses such as rice and wheat, which are economically significant for agriculture. Furthermore, Azospirillum spp. influence gene expression pathways in plants, enhancing their resistance to biotic and abiotic stressors. Advances in genomics and transcriptomics have provided new insights into plant-microbe interactions. This review explored the molecular mechanisms underlying the role of Azospirillum spp. in plant growth. Additionally, BNF phytohormone synthesis, root architecture modification for nutrient uptake and stress tolerance, and immobilization for enhanced crop production are also important. A deeper understanding of the molecular basis of Azospirillum in biofertilizer and biostimulant development, as well as genetically engineered and immobilized strains for improved phosphate solubilization and nitrogen fixation, will contribute to sustainable agricultural practices and help to meet global food security demands. Full article

The wheat curl mite (WCM, Aceria tosichella Keifer), a complex of eriophyid mite species, transmits wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV), which in single or mixed infections cause wheat streak mosaic (WSM) disease—a major threat to wheat production across the U.S. Great Plains. Resistant wheat cultivars bearing Cmc3 and Cmc4 (targeting WCM), Wsm1 and Wsm2 (targeting WSMV), and Wsm1 (targeting TriMV) are widely used to manage this pest–pathogen complex. However, comprehensive studies investigating how these resistance mechanisms influence both vector biology and virus transmission remain scarce. To address this gap, we evaluated disease development and WCM fitness across nine wheat cultivars with differential resistance profiles under single and mixed infections of WSMV and TriMV. We found strong viral synergy in co-infected plants, with TriMV accumulation markedly enhanced during mixed infections, irrespective of host genotype. Symptom severity and virus titers (both WSMV and TriMV) were highest in the cultivars carrying Wsm2, suggesting a potential trade-off in resistance effectiveness under mixed infection pressure. While mite development time (egg to adult) was unaffected by host genotype or infection status, mite fecundity was significantly reduced on infected plants carrying Wsm1 or Wsm2, but not on those with Cmc3 and Cmc4. Notably, virus accumulation in mites was reduced on the cultivars with Cmc3 and Cmc4, correlating with virus titers in the host tissues. Our findings highlight the complex interplay between host resistance, virus dynamics, and vector performance. Cultivars harboring Cmc3 and Cmc4 may offer robust field-level protection by simultaneously suppressing mite reproduction and limiting virus accumulation in both plant and vector. Full article