Search Results (719)

Aiming at tight farming schedules, slow straw decomposition, and severe soil-borne disease risks in the practical maize straw returning production of China’s wheat–maize double cropping zones, this study developed a field-adapted in situ rapid high-temperature straw composting technology matched with a special composite microbial inoculant. Post-harvest summer maize straw collected from the field was crushed to 3–5 cm; the inoculant group T and water control CK were arranged with three biological replicates. Raw materials were adjusted to 65% moisture and loosely stacked into trapezoidal piles equipped with layered temperature–humidity sensors covered by plastic film for continuous monitoring. After formula and pile structure optimization, the pile temperature exceeded 50 °C within 8 h and stayed at 58–63 °C for 9 days, limiting the composting cycle to within 15 days. Cellulose and lignin degradation reached 56.25% and 50.39%, respectively; available P and K rose by 12.33% and 14.69%, free amino acids doubled; the C/N ratio dropped to 18:1 and the GI exceeded 130%. High temperature enriched functional flora of Bacillus subtilis, Aspergillus niger and actinomycetes, whereas pathogenic Fusarium abundance decreased to less than 1/31 of the initial level. This technology can bring approximately 400 yuan of potential additional benefit per mu, providing an efficient and labor-saving practical candidate for straw returning in regions with a high multiple-cropping index. Full article

Wheat is one of the world’s most important cereal crops, and its technological quality is essential for the production of flour, dough and bakery products. Fusarium infection and the associated accumulation of mycotoxins may adversely affect grain composition, processing performance and food safety. This review summarizes current knowledge on the influence of Fusarium infection and associated mycotoxin contamination on the chemical composition and technological quality of wheat. A literature search was conducted in the Web of Science Core Collection, and eligible studies were included in the qualitative synthesis. The reviewed studies demonstrate that Fusarium infection generally reduces grain quality, gluten functionality, dough rheological properties and baking performance, although the magnitude and direction of changes depend on the Fusarium species, wheat cultivar, infection model and mycotoxin concentration. Considerable heterogeneity among experimental designs limits direct comparison of individual studies. By integrating evidence across grain, flour, dough and bread quality parameters, this review provides a comprehensive and comparative synthesis of the effects of different Fusarium species and associated mycotoxins on wheat technological quality and identifies major areas requiring further investigation. Full article

The rising global food demand requires boosting agricultural productivity without compromising environmental sustainability, especially in the face of intensive agrochemical use and soil degradation. Based on this, strain TRQ67 was isolated from wheat rhizosphere soil in the Yaqui Valley, Mexico, and characterized morphologically, biochemically, and genomically. Strain TRQ67 possesses a genome of 4.04 Mbp across 37 contigs with a G + C content of 46.3%, comprising 4127 coding DNA sequences (CDSs), and was identified as Bacillus velezensis through Overall Genome Relatedness Indices (OGRIs), including Average Nucleotide Identity (OrthoANI = 99.12%) and Genome-to-Genome Distance Calculator (Formula 2: 92.6%). The genome revealed key functional genes associated with auxin biosynthesis (trpABCDEF and yhcX), iron acquisition (dhbABF), nutrient solubilization (gabD, acnAB and pyc), stress response (clpCEPX and pspA), antifungal metabolite synthesis (srfAABCD, fenABCD and bmyABC), chemotaxis and motility (cheABCD, motAB, flgBCDEF, swrC), bacterial fitness (acoABR, acuABC and budABC), exopolysaccharide production (epsDEFHI), sporulation (spo0ABEF) and bioremediation. Predicted gene functions were supported by in vitro phenotypic assays; strain TRQ67 was able to solubilize phosphate (Solubilization Index of 4.1 ± 0.46), biosynthesize siderophores (Production Index of 1.70 ± 0.16), and produce indoles (6.52 ± 0.63 µg mL⁻¹). Furthermore, this strain demonstrated antagonistic activity against phytopathogenic fungi Fusarium languescens and Bipolaris sorokiniana, resulting in reductions in fungal growth area of 87.33% and 89.28%, respectively. These antagonistic effects are consistent with the presence of Biosynthetic Gene Clusters (BGCs) encoding lipopeptides (surfactin and fengycin), polyketides (difficidin, bacillaene and macrolactin H), dipeptides (bacilysin) and siderophores (bacillibactin), as identified through antiSMASH analysis. Finally, the strain significantly improved root (27.63%) and shoot (5.82%) biomass in wheat plants under controlled conditions. These results highlight Bacillus velezensis TRQ67 as a promising microbial inoculant with plant growth promotion capabilities and potential antifungal activity against phytopathogenic fungi, as evidenced by strong in vitro antagonistic activity, supporting its further evaluation for sustainable agricultural practices. Full article

Durum wheat is highly susceptible to Fusarium head blight (FHB), a severe fungal disease caused primarily by Fusarium graminearum. FHB affects durum wheat production by causing significant yield losses and grain contamination with mycotoxins such as deoxynivalenol (DON) and zearalenone (ZEA). Given the limitations of chemical fungicides, finding sustainable biological control agents is essential. This study evaluated the antagonistic and biocontrol capabilities of Trichoderma harzianum ITEM 3636 against F. graminearum through in vitro and greenhouse experiments. In vitro dual and sandwich culture assays demonstrated that T. harzianum significantly inhibits pathogen mycelial growth through direct interaction and the emission of volatile compounds. In a competition test on rice kernels, co-inoculation with ITEM 3636 significantly reduced pathogen biomass, leading to maximum reductions of 96.5% for DON and 98% for ZEA. Furthermore, greenhouse trials on a commercial durum wheat cultivar revealed that T. harzianum ITEM 3636 significantly decreased FHB severity by 40% and reduced DON contamination by up to 32% only when a combined seed-coating and spike-spraying application was performed. Additionally, ITEM 3636 exhibited biostimulant-like effects on yield parameters, causing an increase in kernel weight of 33% in the greenhouse assay. These findings highlight T. harzianum ITEM 3636 as a promising and ecological alternative to synthetic fungicides for managing FHB, safeguarding crop production, and ensuring food safety. Full article

Wheat Fusarium head blight (FHB) is a globally prevalent and destructive fungal disease predominantly caused by Fusarium graminearum. Pydiflumetofen, a novel succinate dehydrogenase inhibitor (SDHI) fungicide, exhibits strong inhibitory activity against F. graminearum; however, the molecular regulatory mechanisms underlying the field-developed resistance in this pathogen remain poorly defined. In the present study, a field-evolved resistant isolate W24-039 and a sensitive isolate W24-016 were subjected to multi-omics analysis. The sequencing results identified compound mutations C89S/A93V in SdhC2, and A21T/S30F in SdhD of the resistant strain, which confer stable fungicide resistance without any detectable fitness costs. Physiological tests revealed that these target mutations sustain the homeostasis of succinate dehydrogenase (SDH) activity and intracellular ATP production. Following pydiflumetofen treatment, the sensitive isolate displayed remarkable declines in SDH activity, intracellular ATP content and deoxynivalenol (DON) biosynthesis, accompanied by markedly elevated cell membrane permeability. Transcriptomic sequencing uncovered 2221 differentially expressed genes (DEGs) in the sensitive strain under fungicide stress, and 2566 DEGs in the resistant isolate under the same conditions. The genes associated with detoxification and drug efflux, including cytochrome P450, glutathione S-transferase (GST), ABC and MFS transporters, were significantly upregulated in the resistant isolate. Metabolomic analysis indicated that differential metabolites were mainly enriched in the tricarboxylic acid (TCA) cycle, amino acid metabolism and membrane lipid biosynthesis pathways. The resistant strain maintained intact TCA cycle operation and accumulated high levels of pivotal metabolites such as phosphatidylcholine, unsaturated fatty acids and reduced glutathione. Integrated multi-omics analysis verified that the ABC transporter and glutathione metabolism pathways serve as core regulatory modules governing fungicide resistance. Collectively, F. graminearum develops resistance via the synergistic effects of SDH compound mutations, enhanced detoxification and efflux, and global metabolic remodeling, demonstrating that target-site mutation alone is not the sole driver of resistance, which is instead controlled by an intricate regulatory network involving multiple coordinated pathways. This study systematically characterizes the resistance regulatory network of F. graminearum against pydiflumetofen, and provides theoretical guidance for the rational application and sustainable field resistance management of this fungicide. Full article

Background/Objectives: Wheat root and crown rot, caused by Fusarium pseudograminearum, Fusarium graminearum, and Bipolaris sorokiniana, are devastating soil-borne diseases that cause substantial yield losses worldwide. Endophytic fungi are promising sources of bioactive metabolites for agricultural applications. This study aimed to isolate and characterize an endophytic fungus with antifungal activity against major wheat pathogens, identify its active compound, and investigate the underlying transcriptional response. Methods: An endophytic strain Y2 was isolated from Hedyotis diffusa leaves and identified through morphological and phylogenetic analysis based on TEF-1α and RPB2 sequences. Pathogenicity of strain Y2 was evaluated on wheat stem bases. The bioactive compound was purified by HPLC and identified by HR-ESI-MS and NMR. Antifungal activity was assessed using dual-culture and microbroth dilution assays. Transcriptomic analysis (RNA-seq) was performed on F. pseudograminearum treated with equisetin, with qRT-PCR validation of seven representative differentially expressed genes. Results: Strain Y2 was identified as Fusarium incarnatum or a closely related member of the F. incarnatum–equiseti species complex (FIESC) and confirmed to be non-pathogenic to wheat. The purified bioactive compound was characterized as equisetin, which exhibited significant antifungal activity with MIC values of 16, 32, and 64 μg/mL against F. pseudograminearum, B. sorokiniana, and F. graminearum, respectively. Transcriptomic analysis revealed that equisetin treatment induced a polarized transcriptional response in F. pseudograminearum, characterized by strong upregulation of ribosome and translation-related genes and widespread downregulation of other metabolic pathways, particularly nitrogen metabolism. qRT-PCR validation of seven representative genes confirmed the reliability of the RNA-seq data. Conclusions: Our findings demonstrate that equisetin is the active antifungal metabolite produced by F. incarnatum Y2, with potent in vitro activity against major wheat root and crown rot pathogens. The transcriptomic data provide insights into the potential mechanism of action, while the non-pathogenic nature of strain Y2 supports its biosafety. Although these results highlight equisetin as a promising lead compound for antifungal development, further in planta efficacy and safety studies are required before it can be considered for practical biocontrol. Full article

Fusarium head blight (FHB) caused by Fusarium graminearum is a major disease of wheat and barley worldwide. Besides causing yield loss, F. graminearum also contaminates infected grains with trichothecene mycotoxins such as deoxynivalenol (DON) and its acetylated derivatives. Field isolates of F. graminearum vary in the amount of mycotoxins produced, both on infected wheat heads and in controlled laboratory experiments. Genes encoding the enzymes responsible for trichothecene mycotoxin biosynthesis are already characterized, but additional genes responsible for the variation in amounts of mycotoxins detected within and among populations remain to be identified. We measured levels of trichothecenes produced in vitro in a sample of 151 F. graminearum field isolates. Genome-wide association performed with these measurements identified 10 quantitative trait loci (QTL) associated with variation in DON and/or 15ADON levels. The candidate regions contain many functionally characterized genes, including the Swr1p helicase gene, an MFS transporter, and multiple other transmembrane transporters that may relate to the fungus’ ability to transport trichothecenes across membranes for sequestration or export. These results help to characterize the genetic factors that influence variability in trichothecene levels, which contribute to our understanding of trichothecene levels on infected grain and may lead to strategies to mitigate this toxin contamination. Full article

Fusarium head blight (FHB) caused by Fusarium graminearum leads to huge yield losses and mycotoxin contamination in wheat globally. Paenibacillus polymyxa with strong antagonistic activity was preliminarily identified. To clarify the key antifungal component, an extracellular protein was purified via ammonium sulfate precipitation, DEAE-52 anion-exchange and Sephadex G-75 gel filtration chromatography. SDS-PAGE showed a single band at 76 kDa. liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis confirmed this protein belongs to glycosyl hydrolase family with 86% sequence coverage. Biochemical characterization showed that the crude protein was stable at 40–90 °C and pH 3.0–9.0, sensitive to proteinase K, trypsin and neutral protease. The purified 76 kDa protein exhibited antifungal activity against F. graminearum. The gene encoding this protein was cloned and expressed in Escherichia coli. The renatured recombinant protein p76kd showed comparable antifungal activity to the native protein. This study purified and characterized a 76 kDa protein annotated as a glycosyl hydrolase via LC-MS/MS peptide matching; its antifungal function is presumed to originate from the conserved glycosyl hydrolase domain according to existing homologous research, which is distinct from previously reported lipopeptides or uncharacterized complexes. This protein provides a promising candidate for the biocontrol of FHB and related fungal diseases in cereal crops. Full article

Ganoderma lucidum polysaccharide (GLP) exhibits prominent antibacterial and antioxidant activities. This study evaluated the effects of GLP seed soaking at two concentrations (4 g/100 kg, GLP4; 8 g/100 kg, GLP8) on wheat growth promotion and induced resistance against wheat sharp eyespot caused by Rhizoctonia solani and Fusarium head blight (FHB) mainly caused by Fusarium graminearum. Physiological and agronomic analyses showed that GLP treatment increased the germination rate of all tested wheat cultivars (moderately resistant: Xiaoyan 22, Sumai 3; moderately susceptible: Mingxian 169, Xinong 873) by over 2%. Moderately susceptible and resistant cultivars presented average plant height increases of 0.5 cm and 2 cm, respectively, with most cultivars showing a height increase of approximately 3 cm. GLP significantly elevated leaf chlorophyll content by over 5% and differentially regulated malondialdehyde (MDA) levels: MDA decreased by 34–68% in Sumai 3 and Mingxian 169 but increased by 11–23% in Xiaoyan 22. Pot assays verified that GLP yielded over 10% control efficacy against both diseases. Overall, GLP seed soaking effectively promotes wheat growth, activates defense responses, and enhances host resistance to R. solani and F. graminearum infections. Full article

Fusarium graminearum and F. avenaceum often co-contaminate Canadian durum wheat grain, resulting in the co-deposition of species-specific mycotoxins, including trichothecenes produced by F. graminearum and enniatins produced by F. avenaceum. These mycotoxins pose significant risks to human and animal health. Although these fungi commonly co-occur in infected wheat, relatively little is known about how they interact during host infection. Interactions between the two species were examined using co-inoculation experiments on durum wheat spikes. In pathology trials, co-inoculations often reduced both disease severity and trichothecene accumulation compared with inoculations of F. graminearum alone, despite F. graminearum greatly out-competing F. avenaceum in total fungal biomass. Transcriptomic profiling identified strong induction of the F. graminearum ABC transporter gene FgABCB8 during co-inoculation with an enniatin-producing F. avenaceum strain. When F. graminearum was grown in vitro, FgABCB8 was induced upon exposure to F. avenaceum culture filtrate, or the related cyclohexadepsipeptides enniatin B1 or beauvericin. Heterologous expression of FgABCB8 in yeast provided partial protection against enniatin and beauvericin toxicity. Gene disruption of FgABCB8 increased F. graminearum sensitivity to enniatins. These findings demonstrate that FgABCB8 expression enhances the ability of F. graminearum to tolerate enniatin-producing fungi. Full article

Wild grasses such as Aegilops cylindrica are a promising source of epiphytic bacteria with plant growth-promoting (PGP) properties and those that contribute to biocontrol. Two bacterial strains from the phyllosphere of A. cylindrica were isolated and identified as Bacillus mojavensis WP4 and Bacillus siamensis WS6. Both strains produced auxins (1.56–5.09 μg/mL) and siderophores and dissolved phosphates. They inhibited the growth of F. culmorum in vitro and increased the biomass of wheat seedlings by up to 2.9 times, although the effects were variety-specific (Tulaykovskaya 10 variety). Neither strain exhibited multiple drug resistance. B. mojavensis WP4 and B. siamensis WS6 are multifunctional strains that stimulate plant growth and exhibit biocontrol activity against F. culmorum during the germination stage. But visible protection of young plants (not seeds) was not observed; however, bacterial treatment restored photosystem II activity (Fv/Fm and Y(II)) in infected plants. Microscopic examination confirmed root colonization. Future research should focus, on the one hand, on conducting experiments in greenhouses and under field conditions, and on the other hand, on investigating the molecular mechanisms underlying the interaction between the bacterium (Bacillus sp. strain), the plant (wheat), and the phytopathogen (F. culmorum). Full article

Mycotoxins are toxic secondary metabolites produced primarily by molds of the genera Aspergillus, Fusarium, and Penicillium. These widespread food and feed contaminants can cause significant risks to human and animal health. The aim of this study was to compare the performance of reference chromatographic methods (high-performance liquid chromatography coupled with either fluorescence detection (HPLC-FLD) or tandem mass spectrometry (HPLC-MS/MS)) with two commercially available rapid tests from two manufacturers. To that end, 90 randomly selected grain samples (barley n = 10, wheat n = 21, triticale n = 10, maize n = 49) collected in 2025 were analyzed for deoxynivalenol (DON), zearalenone (ZEN), ochratoxin A (OTA), and the sum of T-2 and HT-2 toxins. None of the samples exceeded the maximum levels established by the European Union (EU); however, widespread contamination with one or more mycotoxins was observed. Results showed that although rapid test strips offer advantages such as low cost, short analysis time, and operational simplicity, their considerably higher limits of detection and quantification values make them unsuitable for advanced laboratory analysis. Therefore, HPLC-FLD and HPLC-MS/MS remain the gold-standard methods for reliable, sensitive, and precise mycotoxin determination. Full article

The increasing demand for sustainable crop protection strategies has intensified interest in plant-beneficial bacteria as alternatives to synthetic agrochemicals. In this study, the soil-derived bacterium Pseudomonas putida V01 was isolated and characterized for its antifungal and plant growth-promoting potential through an integrated approach combining biological assays, untargeted metabolomics, and in vivo plant experiments. Cell-free culture filtrates exhibited strong antifungal activity against major phytopathogenic fungi, completely inhibiting the growth of Sclerotium rolfsii and significantly reducing mycelial development of Alternaria alternata and Fusarium proliferatum by 40% and 20%, respectively. Volatile organic compounds (VOCs) selectively inhibited Botrytis cinerea and A. alternata by 28% and 10%, respectively, and affected sporulation of F. proliferatum. Metabolomic profiling through LC-qTOF-MS and GC-MS analyses revealed a chemically diverse metabolome, including putatively annotated diketopiperazines, cyclic peptides, phenolic compounds, and fatty acids. VOC profiling indicated ketones and alcohols as the predominant volatile classes, with 2-undecanone and 2-undecanol among the most abundant compounds detected. In vivo assays on wheat seedlings showed significant increases in shoot growth, biomass accumulation, and chlorophyll content compared with untreated controls. These findings indicate that P. putida V01 combines complementary antifungal and plant growth-promoting activities associated with a diverse repertoire of diffusible and volatile metabolites. The integrated biological and metabolomic characterization highlights its potential as a multifunctional microbial inoculant for sustainable crop production and disease management. Full article

Recently, mycotoxin prediction has mainly relied on meteorological data and crop physiology. The contribution of soil characteristics as additional environmental variables remains largely unexplored. A systematic literature search was carried out to analyze the latest research (from 2020 to 2025) on the relationship between soil properties (temperature, water content, pH, and electrical conductivity), fungal communities (particularly Aspergillus and Fusarium), and different crops (mainly peanut, wheat, and maize). Measurement methodologies were analyzed, with a focus on the use of in-field soil sensors in correlation studies and predictive models. Disease incidence and mycotoxin occurrence were related to stressful soil conditions, such as different pH levels, wetness or drought, and temperatures above 25 °C. Other external variables (crop and field management) must also be considered. Laboratory equipment was primarily used in correlation studies, with limited in-field sensor implementation. Although recent predictive models included soil properties as effective inputs, they mostly relied on satellite data. However, real-time conditions and fluctuations, which can be captured by in-field soil sensors, are essential for training new functional models. To monitor soil properties, IoT technologies must be considered, but their implementation is still not sufficient to collect widespread data. Therefore, groundwork is needed to fill this gap with high-quality soil data for future in-field experimentation. Full article

Biological control is one of the most effective strategies for managing crop fungal diseases such as rice blast, which severely threatens global food security. However, the limited availability of microbial biocontrol resources and incomplete understanding of their mechanisms hinder the development of practical biocontrol technologies for rice blast. In this study, a Bacillus stercoris strain, JK-6, isolated from the rhizosphere soil of rice, was identified as a promising biocontrol agent with strong antagonistic activity against multiple fungal pathogens. The fermentation broth of JK-6 yielded inhibition rates of 94.96% against Magnaporthe oryzae (rice blast), 75.83% against Bipolaris maydis (maize southern leaf blight), and 70.46% against Fusarium graminearum (wheat head blight). Whole-genome sequencing of JK-6 revealed 12 biosynthetic gene clusters, one of which was responsible for the biosynthesis of the lipopeptide surfactin. Further assays showed that 200 μM surfactin exhibited broad-spectrum antifungal activity, with inhibition rates of 82.90%, 66.76%, and 52.54% against M. oryzae, B. maydis, and F. graminearum, respectively. Mechanistically, surfactin suppresses fungal growth by downregulating genes involved in integral and intrinsic membrane components and oxygen transport, as validated by transcriptomic analysis. Our discoveries not only advance the conceptual understanding of the surfactin-mediated JK-6 antagonistic activity against fungal diseases but also offer an effective new approach for the practical control of crop fungal diseases. Full article