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16 pages, 950 KiB  
Article
Survey of Weed Flora Diversity as a Starting Point for the Development of a Weed Management Strategy for Medicinal Crops in Pančevo, Serbia
by Dragana Božić, Ana Dragumilo, Tatjana Marković, Urban Šilc, Svetlana Aćić, Teodora Tojić, Miloš Rajković and Sava Vrbničanin
Horticulturae 2025, 11(8), 882; https://doi.org/10.3390/horticulturae11080882 - 31 Jul 2025
Viewed by 176
Abstract
Similarly to conventional field crops, weeds often pose significant problems in the cultivation of medicinal plants. To date, no comprehensive documentation exists regarding weed infestation levels in these crops in Serbia. The objective of this study was to provide a valuable foundation for [...] Read more.
Similarly to conventional field crops, weeds often pose significant problems in the cultivation of medicinal plants. To date, no comprehensive documentation exists regarding weed infestation levels in these crops in Serbia. The objective of this study was to provide a valuable foundation for developing effective, site-specific weed management strategies in medicinal crop production. Weeds in five medicinal crops (lemon balm, fennel, peppermint, ribwort plantain, German chamomile), were surveyed based on the agro-phytosociological method between 2019 and 2024, and across 59 plots. A total of 109 weed species were recorded, belonging to 29 families and 88 genera. Among them, 75 were annuals and 34 perennials, including 93 broadleaved species, 10 grasses, and one parasitic species. All surveyed plots were heavily infested with perennial weeds such as Elymus repens, Cirsium arvense, Convolvulus arvensis, Lepidium draba, Rumex crispus, Sorghum halepense, Taraxacum officinale, etc. Also, several annual species were found in high abundance and frequency, including Amaranthus retroflexus, Chenopodium album, Galium aparine, Lactuca serriola, Lamium amplexicaule, L. purpureum, Papaver rhoeas, Stellaria media, Veronica hederifolia, V. persica, etc. The most important ecological factors influencing the composition of weed vegetation in investigated medicinal crops were temperature and light for fennel and peppermint plots, soil reaction for lemon balm and ribwort plantain plots, and nutrient content for German chamomile plots. A perspective for exploitation of these results is the development of effective weed control programs tailored to this specific cropping system. Weed control strategies should consider such information, targeting the control of the most frequent, abundant, and dominant species existing in a crops or locality. Full article
(This article belongs to the Special Issue Conventional and Organic Weed Management in Horticultural Production)
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22 pages, 3604 KiB  
Article
Succession of Weed Community on Wheat Lands in the Past 25 Years: A Case Study in Eastern China
by Guoqi Chen, Zeyue Huang, Jiahao Xue, Feng Zhu, Yang Chen and Yunfei Wu
Biology 2025, 14(8), 943; https://doi.org/10.3390/biology14080943 - 27 Jul 2025
Viewed by 238
Abstract
Weeds represent increasingly troublesome threats to modern wheat production. Jiangsu Province, China, is one of the largest wheat-planting areas, characterized by a high level of agricultural mechanization, in the country. In 2024, we surveyed weed communities of 924 wheat lands from 308 sites [...] Read more.
Weeds represent increasingly troublesome threats to modern wheat production. Jiangsu Province, China, is one of the largest wheat-planting areas, characterized by a high level of agricultural mechanization, in the country. In 2024, we surveyed weed communities of 924 wheat lands from 308 sites in Jiangsu and compared them with historical data surveyed in 1999–2000 in this province. A total of 156 weed species belonging to 103 genera and 39 families were recorded. Compositae and Poaceae showed the highest species richness, with 26 and 23 species, respectively. Poaceae weeds accounted for 54.3% of the overall weeds in dominance values. Beckmannia syzigachne showed the highest proportion of the overall weeds in dominance values (19.2%). Compared with the historical data, weed diversity increased by 33.3% in species, and by 62.5% in families; grassy weeds such as Beckmannia syzigachne, Alopecurus japonicus, and Alopecurus myosuroides became more dominant, while several low-growing grassy weeds became less dominant. The dominance of broadleaf weeds, such as Galium spp., Vicia spp., and Veronica spp., decreased greatly, and a clear trend of weed homogenization among different areas and types of wheat lands was observed. This is the first investigation of weed community succession against the background of agricultural modernization in China. Full article
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32 pages, 1770 KiB  
Article
Regional Patterns in Weed Composition of Maize Fields in Eastern Hungary: The Balance of Environmental and Agricultural Factors
by Mihály Zalai, Erzsébet Tóth, János György Nagy and Zita Dorner
Agronomy 2025, 15(8), 1814; https://doi.org/10.3390/agronomy15081814 - 26 Jul 2025
Viewed by 460
Abstract
The primary aim of this study was to explore the influence of abiotic factors on weed development in maize fields, with the goal of informing more effective weed management practices. We focused on identifying key environmental, edaphic, and agricultural variables that contribute to [...] Read more.
The primary aim of this study was to explore the influence of abiotic factors on weed development in maize fields, with the goal of informing more effective weed management practices. We focused on identifying key environmental, edaphic, and agricultural variables that contribute to weed infestations, particularly before the application of spring herbicide treatments. Field investigations were conducted from 2018 to 2021 across selected maize-growing regions in Hungary. Over the four-year period, a total of 51 weed species were recorded, with Echinochloa crus-galli, Chenopodium album, Portulaca oleracea, and Hibiscus trionum emerging as the most prevalent taxa. Collectively, these four species accounted for more than half (52%) of the total weed cover. Altogether, the 20 most dominant species contributed 95% of the overall weed coverage. The analysis revealed that weed cover, species richness, and weed diversity were significantly affected by soil properties, nutrient levels, geographic location, and tillage systems. The results confirm that the composition of weed species was influenced by several environmental and management-related factors, including soil parameters, geographical location, annual precipitation, tillage method, and fertilizer application. Environmental factors collectively explained a slightly higher proportion of the variance (13.37%) than farming factors (12.66%) at a 90% significance level. Seasonal dynamics and crop rotation history also played a notable role in species distribution. Nutrient inputs, particularly nitrogen, phosphorus, and potassium, influenced both species diversity and floristic composition. Deep tillage practices favored the proliferation of perennial species, whereas shallow cultivation tended to promote annual weeds. Overall, the composition of weed vegetation proved to be a valuable indicator of site-specific soil conditions and agricultural practices. These findings underscore the need to tailor weed management strategies to local environmental and soil contexts for sustainable crop production. Full article
(This article belongs to the Special Issue State-of-the-Art Research on Weed Populations and Community Dynamics)
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35 pages, 6030 KiB  
Review
Common Ragweed—Ambrosia artemisiifolia L.: A Review with Special Regards to the Latest Results in Protection Methods, Herbicide Resistance, New Tools and Methods
by Bence Knolmajer, Ildikó Jócsák, János Taller, Sándor Keszthelyi and Gabriella Kazinczi
Agronomy 2025, 15(8), 1765; https://doi.org/10.3390/agronomy15081765 - 23 Jul 2025
Viewed by 435
Abstract
Common ragweed (Ambrosia artemisiifolia L.) has been identified as one of the most harmful invasive weed species in Europe due to its allergenic pollen and competitive growth in diverse habitats. In the first part of this review [Common Ragweed—Ambrosia artemisiifolia L.: [...] Read more.
Common ragweed (Ambrosia artemisiifolia L.) has been identified as one of the most harmful invasive weed species in Europe due to its allergenic pollen and competitive growth in diverse habitats. In the first part of this review [Common Ragweed—Ambrosia artemisiifolia L.: A Review with Special Regards to the Latest Results in Biology and Ecology], its biological characteristics and ecological behavior were described in detail. In the current paper, control strategies are summarized, focusing on integrated weed management adapted to the specific habitat where the species causes damage—arable land, semi-natural vegetation, urban areas, or along linear infrastructures. A range of management methods is reviewed, including agrotechnical, mechanical, physical, thermal, biological, and chemical approaches. Particular attention is given to the spread of herbicide resistance and the need for diversified, habitat-specific interventions. Among biological control options, the potential of Ophraella communa LeSage, a leaf beetle native to North America, is highlighted. Furthermore, innovative technologies such as UAV-assisted weed mapping, site-specific herbicide application, and autonomous weeding robots are discussed as environmentally sustainable tools. The role of legal regulations and pollen monitoring networks—particularly those implemented in Hungary—is also emphasized. By combining traditional and advanced methods within a coordinated framework, effective and ecologically sound ragweed control can be achieved. Full article
(This article belongs to the Section Weed Science and Weed Management)
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20 pages, 2970 KiB  
Review
The Rise of Eleusine indica as Brazil’s Most Troublesome Weed
by Ricardo Alcántara-de la Cruz, Laryssa Barbosa Xavier da Silva, Hudson K. Takano, Lucas Heringer Barcellos Júnior and Kassio Ferreira Mendes
Agronomy 2025, 15(8), 1759; https://doi.org/10.3390/agronomy15081759 - 23 Jul 2025
Viewed by 577
Abstract
Goosegrass (Eleusine indica) is a major weed in Brazilian soybean, corn, and cotton systems, infesting over 60% of grain-producing areas and potentially reducing yields by more than 50%. Its competitiveness is due to its rapid emergence, fast tillering, C4 metabolism, and [...] Read more.
Goosegrass (Eleusine indica) is a major weed in Brazilian soybean, corn, and cotton systems, infesting over 60% of grain-producing areas and potentially reducing yields by more than 50%. Its competitiveness is due to its rapid emergence, fast tillering, C4 metabolism, and adaptability to various environmental conditions. A critical challenge relates to its widespread resistance to multiple herbicide modes of action, notably glyphosate and acetyl-CoA carboxylate (ACCase) inhibitors. Resistance mechanisms include 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) target-site mutations, gene amplification, reduced translocation, glyphosate detoxification, and mainly ACCase target-site mutations. This literature review summarizes the current knowledge on herbicide resistance in goosegrass and its management in Brazil, with an emphasis on integrating chemical and non-chemical strategies. Mechanical and physical controls are effective in early or local infestations but must be combined with chemical methods for lasting control. Herbicides applied post-emergence of weeds, especially systemic ACCase inhibitors and glyphosate, remain important tools, although widespread resistance limits their effectiveness. Sequential applications and mixtures with contact herbicides such as glufosinate and protoporphyrinogen oxidase (PPO) inhibitors can improve control. Pre-emergence herbicides are effective when used before or immediately after planting, with adequate soil moisture being essential for their activation and effectiveness. Given the complexity of resistance mechanisms, chemical control alone is not enough. Integrated weed management programs, combining diverse herbicides, sequential treatments, and local resistance monitoring, are essential for sustainable goosegrass management. Full article
(This article belongs to the Section Weed Science and Weed Management)
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25 pages, 4261 KiB  
Article
Influence of Mulching and Planting Density on Agronomic and Economic Traits of Melissa officinalis L.
by Stefan V. Gordanić, Dragoja Radanović, Miloš Rajković, Milan Lukić, Ana Dragumilo, Snežana Mrđan, Petar Batinić, Natalija Čutović, Sara Mikić, Željana Prijić and Tatjana Marković
Horticulturae 2025, 11(8), 866; https://doi.org/10.3390/horticulturae11080866 - 22 Jul 2025
Viewed by 403
Abstract
Melissa officinalis L. (Lamiaceae) is a perennial plant species widely used in the pharmaceutical and food industries, particularly valued for its sedative properties. This study investigates the impact of synthetic mulch film and planting density as two experimental factors on agronomic performance, raw [...] Read more.
Melissa officinalis L. (Lamiaceae) is a perennial plant species widely used in the pharmaceutical and food industries, particularly valued for its sedative properties. This study investigates the impact of synthetic mulch film and planting density as two experimental factors on agronomic performance, raw material quality, and economic efficiency in lemon balm production. The experiment was conducted at three locations in Serbia (L1: Bačko Novo Selo, L2: Bavanište, L3: Vilandrica) from 2022 to 2024, using two planting densities on synthetic mulch film (F1: 8.3 plants m−2; F2: 11.4 plants m−2) and a control treatment without mulch (C). The synthetic mulch film used was a synthetic black polypropylene film (Agritela Black, 90 g/m2), uniformly applied in strips across the cultivation area, covering approximately 78% of the soil surface. The results showed consistent increases in morphological parameters and yield across the years. Plant height in F1 and F2 treatments ranged from 65 to 75 cm, while in the control it reached up to 50 cm (2022–2024). Fresh biomass yield varied from 13.4 g per plant (C) to 378.08 g per plant (F2), and dry biomass yield from 60.3 g (C) to 125.4 g (F2). The highest essential oil content was observed in F2 (1.2% in 2022), while the control remained at 0.8%. The F2 treatment achieved complete weed suppression throughout the experiment without the use of herbicides, demonstrating both agronomic and ecological advantages. Economic evaluation revealed that F2 generated the highest cumulative profit (€142,164.5) compared to the control (€65,555.3). Despite higher initial investment, F2 had the most favorable cost–benefit ratio in the long term. This study highlights the crucial influence of mulching and planting density on optimizing lemon balm production across diverse climatic and soil conditions, while also underscoring the importance of sustainable, non-chemical weed management strategies in lemon balm cultivation. Full article
(This article belongs to the Special Issue Conventional and Organic Weed Management in Horticultural Production)
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22 pages, 6781 KiB  
Article
Seasonal Variation in Flower Traits, Visitor Traits, and Reproductive Success of Solanum sisymbriifolium Lamarck (Solanaceae) in the Rarh Region of West Bengal, India
by Ujjwal Layek, Pappu Majhi, Alokesh Das, Prakash Karmakar and Arijit Kundu
Biology 2025, 14(7), 865; https://doi.org/10.3390/biology14070865 - 16 Jul 2025
Viewed by 867
Abstract
The wild tomato (Solanum sisymbriifolium) is a globally distributed shrubby weed with both negative and positive impacts, including its invasive properties and the potential for pharmaceutical and traditional medicinal uses. Despite its ecological significance, the plant’s reproductive biology and pollination ecology [...] Read more.
The wild tomato (Solanum sisymbriifolium) is a globally distributed shrubby weed with both negative and positive impacts, including its invasive properties and the potential for pharmaceutical and traditional medicinal uses. Despite its ecological significance, the plant’s reproductive biology and pollination ecology remain poorly understood. This study aimed to investigate the floral biology, pollination ecology, and plant reproduction of the weed species. Some flower traits, such as flowering intensity, flower display size, and pollen and ovule production, peaked during spring, summer, and the monsoon, while flower longevity and stigmatic receptivity were the longest in winter. The plant species was self-compatible (ISI = 0.02), heavily depended on pollinators (IDP = 0.72), and experienced minimal pollination limitation (D = 0.10) under open-pollination conditions. Flower visitors’ traits (e.g., abundance, diversity, and richness) were higher in the spring, summer, and the monsoon, and these were lower in winter. The vital pollination service was provided by Amegilla zonata, Ceratina binghami, Lasioglossum cavernifrons, Nomia (Curvinomia) strigata, Tetragonula pagdeni, Xylocopa aestuans, Xylocopa amethystina, Xylocopa fenestrata, and Xylocopa latipes. Reproductive success, as indicated by fruit and seed set, varied seasonally, being higher during the spring–monsoon period and lower in winter. These findings support effective management of this weed species and help conserve the associated bee populations. Full article
(This article belongs to the Special Issue Pollination Biology)
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16 pages, 743 KiB  
Article
Effects of Non-Inversion Tillage and Cover Crops on Weed Diversity and Density in Southeastern Romania
by Mădălin Radu, Ciprian Bolohan, Costel Mihalașcu, Andrei Măruțescu, Max John Newbert and Vasileios P. Vasileiadis
Sustainability 2025, 17(13), 6204; https://doi.org/10.3390/su17136204 - 7 Jul 2025
Viewed by 471
Abstract
Conservation agriculture is increasingly recognized as a sustainable alternative to conventional farming in temperate regions due to its benefits in terms of reducing soil erosion, enhancing water retention, and mitigating climate change. Despite these benefits, these practices are not broadly adopted, partially due [...] Read more.
Conservation agriculture is increasingly recognized as a sustainable alternative to conventional farming in temperate regions due to its benefits in terms of reducing soil erosion, enhancing water retention, and mitigating climate change. Despite these benefits, these practices are not broadly adopted, partially due to perceived weed management challenges in conservation systems. This paper explores how a conservation system that uses cover crops and non-inversion tillage (chiselling) influences the weed flora abundance and evolution before cover crop termination and over a complete rotation cycle (sunflower–winter wheat–maize–sunflower) in southeastern Romania when compared to conventional tillage (ploughing). Overall, the conservation system significantly reduced weed density by 31%, preserving a higher diversity and evenness (H′ = 0.75, E = 0.46) by the end of the rotation cycle and an evenly distributed weed community compared to the conventional system, where the opportunistic species Veronica hederifolia exhibited dominance. Full article
(This article belongs to the Special Issue Sustainable Management: Plant, Biodiversity and Ecosystem)
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25 pages, 3047 KiB  
Article
Fate of Pyrrolizidine Alkaloids in Soil: Insights from Myosotis arvensis L. and Senecio vulgaris L.
by Ilva Nakurte, Gundars Skudriņš and Ieva Mežaka
Toxins 2025, 17(7), 335; https://doi.org/10.3390/toxins17070335 - 2 Jul 2025
Viewed by 428
Abstract
Pyrrolizidine alkaloids are plant-derived toxins with environmental persistence and the potential to contaminate soil, water, and adjacent crops. This study investigated the leaching behavior and environmental fate of PAs from two PA-producing weeds—Myosotis arvensis L. (Boraginaceae) and Senecio vulgaris L. (Asteraceae)—in two [...] Read more.
Pyrrolizidine alkaloids are plant-derived toxins with environmental persistence and the potential to contaminate soil, water, and adjacent crops. This study investigated the leaching behavior and environmental fate of PAs from two PA-producing weeds—Myosotis arvensis L. (Boraginaceae) and Senecio vulgaris L. (Asteraceae)—in two Latvian agricultural soils: sandy loam and loam. Hot- and cold-water plant extracts were applied to soil columns (10 cm and 20 cm), and leachates were analyzed over a 14-day period using QuEChERS purification and LC-HRMS detection. Leaching varied by plant species, extract type, and soil. M. arvensis showed significantly higher cumulative leaching (77–84% for cold, 65–71% for hot extracts), attributed to the higher solubility of N-oxides. In contrast, S. vulgaris extracts leached minimally (<0.84% from sandy loam) and were undetectable in loam. The presence of cyclic diester PAs in S. vulgaris and the higher cation exchange capacity of loam favored retention or degradation. PANO-to-PA conversion occurred in both soils, indicating redox activity. The fate of PAs was influenced by structural type (diesters showing higher persistence), extraction method (hot extraction releasing more pyrrolizidine alkaloids), and soil properties such as pH, organic matter, and cation exchange capacity, which affected sorption and mobility. These findings underscore the significance of soil composition in controlling PA mobility and associated environmental risks. Future research should focus on long-term PA persistence across diverse soil types and investigate crop uptake potential and microbial degradation pathways under field conditions. Full article
(This article belongs to the Special Issue Toxic Plant-Derived Metabolites)
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21 pages, 2531 KiB  
Article
Processing Tomato Crop Benefits from Flowering Plants in Field Margins That Support Pollinators and Natural Enemies
by Vaya Kati, Theodoros Stathakis, Leonidas Economou, Philippos Mylonas, Myrto Barda, Theodoros Angelioudakis, Athanasia Bratidou Parlapani, Ilias Tsamis and Filitsa Karamaouna
Agronomy 2025, 15(7), 1558; https://doi.org/10.3390/agronomy15071558 - 26 Jun 2025
Viewed by 471
Abstract
In a two-year experiment, we examined whether increasing plant diversity in the margins of processing tomato fields could attract pollinators and natural enemies of pests compared to weed flora, and questioned the effect on crop yield. Two plant mixtures sown in winter (WM) [...] Read more.
In a two-year experiment, we examined whether increasing plant diversity in the margins of processing tomato fields could attract pollinators and natural enemies of pests compared to weed flora, and questioned the effect on crop yield. Two plant mixtures sown in winter (WM) and spring (SM) were compared with weed vegetation along a tomato crop (CT) and an adjacent irrigation channel (CC). Flower cover was higher in the sown mixtures than the weedy margins, and brought in more visits of pollinating bees (including potential tomato pollinators) than the latter. Flowering species were mainly Eruca vesicaria (WM, SM), Coriandrum sativum and Lathyrus sativus (WM), Fagopyron esculentum and Phacelia tanacetifolia (SM), and Ammi majus, Rapistrum rugosum (CC, CT). Parasitoids (Eulophidae, Braconidae, Scelionidae) were more abundant in the sown and CC margins compared to the CT margin, while the abundance of predators (Aeolothripidae, Orius sp., Thomisidae) was similar among all types of margins. Fruit weight was higher in the field with the sown margins, while pest incidence in the crop was not affected by the margin type. Our findings provide new insights into the contribution of managed and existing field margins in attracting beneficial arthropods, and their implications on yield. Full article
(This article belongs to the Special Issue Pests, Pesticides, Pollinators and Sustainable Farming)
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19 pages, 2927 KiB  
Article
Restoration, Indicators, and Participatory Solutions: Addressing Water Scarcity in Mediterranean Agriculture
by Enrico Vito Perrino, Pandi Zdruli, Lea Piscitelli and Daniela D’Agostino
Agronomy 2025, 15(7), 1517; https://doi.org/10.3390/agronomy15071517 - 22 Jun 2025
Viewed by 516
Abstract
Agricultural water resource management is increasingly challenged by climate variability, land degradation, and socio-economic pressures, particularly in the Mediterranean region. This study, conducted in 2023–2024 within the REACT4MED project (PRIMA initiative), addresses sustainable water use through a comparative analysis of organic and conventional [...] Read more.
Agricultural water resource management is increasingly challenged by climate variability, land degradation, and socio-economic pressures, particularly in the Mediterranean region. This study, conducted in 2023–2024 within the REACT4MED project (PRIMA initiative), addresses sustainable water use through a comparative analysis of organic and conventional farms in the Stornara and Tara area (Puglia, Italy). The research aimed to identify critical indicators for sustainable water management and develop ecosystem restoration strategies that can be replicated across similar Mediterranean agro-ecosystems. An interdisciplinary, participatory approach was adopted, combining technical analyses and stakeholder engagement through three workshops involving 30 participants from diverse sectors. Fieldwork and laboratory assessments included soil sampling and analysis of parameters such as pH, electrical conductivity, soil organic carbon, nutrients, and salinity. Cartographic studies of vegetation, land use, and pedological characterization supplemented the dataset. The key challenges identified were water loss in distribution systems, seawater intrusion, water pumping from unauthorized wells, and inadequate public policies. Soil quality was significantly influenced by salt stress, hence affecting crop productivity, while socio-economic factors affected farm income. Restoration strategies emphasized the need for water-efficient irrigation, less water-intensive crops, and green vegetation in infrastructure channels while incorporating also the native flora. Enhancing plant biodiversity through weed management in drainage channels proved beneficial for pathogen control. Proposed socio-economic measures include increased inclusion of women and youth in agricultural management activities. Integrated technical and participatory approaches are essential for effective water resource governance in Mediterranean agriculture. This study offers scalable, context-specific indicators and solutions for sustainable land and water management in the face of ongoing desertification and climate stress. Full article
(This article belongs to the Section Water Use and Irrigation)
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28 pages, 4157 KiB  
Article
Comprehensive Analysis of Genetic and Morphological Diversity in Echinochloa spp. Populations Infesting Paddy Fields in Ningxia, China
by Jinhui Li, Yi Zhang, Yan Liu, Shouhui Wei, Zhaofeng Huang, Lu Chen and Hongjuan Huang
Int. J. Mol. Sci. 2025, 26(12), 5623; https://doi.org/10.3390/ijms26125623 - 12 Jun 2025
Viewed by 355
Abstract
Barnyard grass is the most problematic weed in paddy fields in Ningxia. Its substantial morphological variation complicates both identification and control, yet the genetic diversity of barnyard grass infesting paddy fields in Ningxia has not been thoroughly studied. In this research, we analyzed [...] Read more.
Barnyard grass is the most problematic weed in paddy fields in Ningxia. Its substantial morphological variation complicates both identification and control, yet the genetic diversity of barnyard grass infesting paddy fields in Ningxia has not been thoroughly studied. In this research, we analyzed the genetic diversity of 46 barnyard grass populations from Ningxia’s paddy fields based on the assessment of morphological traits, DNA barcoding, and SCoT-targeted gene markers. Nine morphological traits were quantitatively analyzed, among which three phenological traits, i.e., leaf length, stem diameter, and plant height, exhibited notable variations. Correlational analysis revealed a positive relationship between morphological traits and multi-herbicide resistance profiles. To assess genetic diversity, four DNA barcodes (ITS, psbA, matK, and trnL-F) were used, among which ITS demonstrated the strongest potential in single-gene barcoding for barnyard grass species identification. Cluster analysis based on ITS barcode sequences was performed to group the populations into five main categories. Additionally, SCoT marker analysis using six primers was performed to classify the 46 barnyard grass samples into five groups. The results showed that the predominant barnyard grass species in Ningxia were E. colona, E. crus-galli var. Formosensis, E. crusgalli, E. oryzoides, and E. crusgalli var. Zelayensis, with E. colona being the most prevalent. The differences observed between the morphological and molecular marker-based classifications were method-dependent. However, both SCoT molecular marker technology and DNA barcoding contributed to identifying the genetic diversity of barnyard grass. Taken together, our study revealed significant morphological and genetic variations among barnyard grass populations, which correlated with herbicide sensitivity in Ningxia’s paddy fields, underscoring the necessity for an integrated weed management approach to combat this troublesome weed species. Full article
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30 pages, 4591 KiB  
Article
Evolution of the Soil Bacterial Community as a Function of Crop Management: A Metagenomic Study in Orange Tree (Citrus sinensis) Plantations
by Carlos Giménez-Valero, Alejandro Andy Maciá-Vázquez, Dámaris Núñez-Gómez, Agustín Conesa, Vicente Lidón and Pablo Melgarejo
Plants 2025, 14(12), 1781; https://doi.org/10.3390/plants14121781 - 11 Jun 2025
Viewed by 447
Abstract
Soil management significantly influences the structure and diversity of soil bacterial communities, affecting biodiversity and ecosystem functions. In semi-arid regions, water efficiency strategies like anti-weed netting are implemented, but their impact on soil microbial communities remains underexplored. This study evaluates the temporal evolution [...] Read more.
Soil management significantly influences the structure and diversity of soil bacterial communities, affecting biodiversity and ecosystem functions. In semi-arid regions, water efficiency strategies like anti-weed netting are implemented, but their impact on soil microbial communities remains underexplored. This study evaluates the temporal evolution of soil bacterial communities in orange tree (Citrus sinensis (L.) Osbeck) plantations under two conditions: with and without anti-weed netting. Soil samples were collected at three time points over a period of 18 months since the establishment of the crop and analyzed using high-throughput 16S rRNA sequencing, assessing alpha and beta diversity, taxonomic composition, and functional pathways via KEGG analysis. The results indicate that weed control netting contributes to stabilizing bacterial diversity over time and increases the relative abundance of dominant phyla such as Planctomycetota, Proteobacteria, Bacteroidota, and Acidobacteriota. Functional predictions revealed significant differences in metabolic pathways, including those associated with nitrogen fixation and organic matter degradation. These findings suggest that anti-weed netting not only influences the taxonomic composition of soil bacterial communities but also modulates their functional potential, with implications for sustainable agriculture in semi-arid environments. This study provides new insights into the interaction between soil management and soil bacterial communities, offering valuable information for optimizing agricultural practices and soil conservation strategies. Full article
(This article belongs to the Section Plant–Soil Interactions)
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16 pages, 1810 KiB  
Article
Occurrence and Genetic Variation of Monolepta hieroglyphica (Motschulsky, 1858) (Coleoptera: Chrysomelidae), a Newly Emerging Pest, Among Hosts in Northeast China
by Wei Sun, Xiuhua Zhang, Jiachun Zhou and Yuebo Gao
Insects 2025, 16(6), 605; https://doi.org/10.3390/insects16060605 - 8 Jun 2025
Viewed by 1113
Abstract
The northeast region of China plays a crucial role in crop production. The leaf beetle Monolepta hieroglyphica (Motschulsky, 1858) (Coleoptera: Chrysomelidae) has emerged as a potential threat to food security in the region. With a wide distribution spanning Asia and Russia, this beetle [...] Read more.
The northeast region of China plays a crucial role in crop production. The leaf beetle Monolepta hieroglyphica (Motschulsky, 1858) (Coleoptera: Chrysomelidae) has emerged as a potential threat to food security in the region. With a wide distribution spanning Asia and Russia, this beetle affects various crops. However, limited information is available regarding its occurrence patterns and genetic diversity among major crops in the region. Based on systematic observations across various hosts, coupled with genetic variation analysis using mitochondrial DNA markers, the main results were as follows. Leaf beetle occurrence varied among hosts, peaking from late July to mid-August, with maize and soybean fields exhibiting higher infestation rates compared with other crops. Notably, late-cultivated maize fields harbored the highest beetle numbers due to the species’ preference for young leaves. The host transfer trajectory may have originated in soybean and weeds, with subsequent alternation between host plants and other crops, before the final migration to cabbage and late-cultivated maize fields. Genetic analysis revealed nine COI haplotypes, four COII haplotypes, eleven Cytb haplotypes, and twenty-one combined haplotypes. No clear relationship existed between genetic diversity and occurrence, and no distinct host-based genetic patterns emerged from neighbor-joining tree and haplotype network analyses. High gene flow rates were observed, likely contributing to decreased genetic variation. An analysis of molecular variance results indicated major genetic variation within populations, although genetic distance and haplotype distribution indicated divergence among host populations. These results provide foundational data for developing effective M. hieroglyphica pest management strategies. Full article
(This article belongs to the Special Issue Corn Insect Pests: From Biology to Control Technology)
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29 pages, 3586 KiB  
Article
Influence of Prosulfocarb and Polymer Supplementation on Soil Bacterial Diversity in Triticum aestivum L. Cultivation
by Małgorzata Baćmaga, Jadwiga Wyszkowska and Jan Kucharski
Int. J. Mol. Sci. 2025, 26(12), 5452; https://doi.org/10.3390/ijms26125452 - 6 Jun 2025
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Abstract
Despite their effectiveness in eliminating weeds, herbicides can indirectly and directly affect organisms, leading to a decline in species abundance as well as disruptions to the structure and functioning of ecosystems. Boxer 800 EC, whose active ingredient is prosulfocarb, is an active herbicide [...] Read more.
Despite their effectiveness in eliminating weeds, herbicides can indirectly and directly affect organisms, leading to a decline in species abundance as well as disruptions to the structure and functioning of ecosystems. Boxer 800 EC, whose active ingredient is prosulfocarb, is an active herbicide commonly used for weed control, but its potential ecological risks are not well understood. With this in mind, a study was conducted to evaluate the effectiveness of sodium alginate and sodium polyacrylate in restoring homeostasis to soil exposed to Boxer 800 EC herbicide. This involved a two-factor pot experiment: factor I—herbicide dose (0.0, 0.8, 4.8, and 48.0 mg kg−1 d.m.); factor II—polymer type (soil with the polymer additives sodium alginate, and sodium polyacrylate). The experiment was carried out on Eutric Cambisols with four replicates and lasted for 50 days. The test plant was Triticum aestivum L., cultivar “KWS Dorium C1”. The contaminant herbicide doses inhibited the proliferation of organotrophic bacteria and actinobacteria and reduced the colony development index (CD) and ecophysiological diversity index (EP) values for these microorganisms. The addition of sodium alginate to the soil increased the proliferation of these microorganisms, whereas sodium polyacrylate inhibited their development. Sodium alginate also increased the colony development index value of organotrophic bacteria and actinobacteria. Across all the analyzed factors, bacteria from the phylum Proteobacteriota dominated. However, the presence of herbicides and polymers changed the abundance of these bacteria. Bacteria of the genus Sphingomonas were the most prevalent genus in the samples. The herbicide Boxer 800 EC exerted a toxic effect on the growth and development of spring wheat, which was reflected in the plant biomass yield (shoot and ear) and the SPAD index. The recommended herbicide dose (0.80 mg kg−1) did not cause significant changes in the growth and development of spring wheat. The hydrogel control additives deepened the negative effect of the herbicide on plant development. While the herbicide significantly reduced the levels of available carbon and total nitrogen in the soil, the polymers increased these parameters. Full article
(This article belongs to the Special Issue Microorganisms in the Environment)
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