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Keywords = water-soluble dietary fiber

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15 pages, 1253 KiB  
Article
Effect of Modification Methods on Composition and Technological Properties of Sea Buckthorn (Hippophae rhamnoides L.) Pomace
by Gabrielė Kaminskytė, Jolita Jagelavičiūtė, Loreta Bašinskienė, Michail Syrpas and Dalia Čižeikienė
Appl. Sci. 2025, 15(15), 8722; https://doi.org/10.3390/app15158722 - 7 Aug 2025
Abstract
With the growth of the plant-based food sector, increasing amounts of by-products are generated. Sea buckthorn pomace (SBP), a by-product of juice and other manufacturing products, is rich in bioactive compounds such as phenolics, oligosaccharides, proteins, and dietary fiber. The aim of the [...] Read more.
With the growth of the plant-based food sector, increasing amounts of by-products are generated. Sea buckthorn pomace (SBP), a by-product of juice and other manufacturing products, is rich in bioactive compounds such as phenolics, oligosaccharides, proteins, and dietary fiber. The aim of the study was to evaluate the impact of modification methods, such as enzymatic hydrolysis and supercritical carbon dioxide extraction (SFE-CO2), on the chemical composition and technological properties of SBP. SBP and SBP obtained after SFE-CO2 (SBP-CO2) were enzymatically modified using Pectinex® Ultra Tropical, Viscozyme® L, and Celluclast® 1.5 L (Novozyme A/S, Bagsværd, Denmark). The SBP’s main constituent was insoluble dietary fiber (IDF), followed by crude proteins and lipids (respectively, 58.7, 21.1 and 12.6 g/100 in d.m.). SFE-CO2 reduced the lipid content (by 85.7%) in the pomace while increasing protein and TDF content. Enzymatic hydrolysis decreased the content of both soluble dietary fiber (SDF) and IDF, and increased the content of mono- and oligosaccharides as well as free phenolics, depending on the commercial enzyme preparation used in SBP and SBP-CO2 samples. Celluclast® 1.5 L was the most effective in hydrolyzing IDF, while Viscozyme® L and Pectinex® Ultra Tropical were the most effective in degrading SDF. Enzymatic treatment improved water swelling capacity, water retention capacity, water solubility index, oil retention capacity of SBP and SBP-CO2; however, it did not have a significant effect on the stability of the emulsions. Modification of SBP by SFE-CO2 effectively increased WSC and WSI, however it reduced WRC. These findings highlight the potential of targeted modifications to enhance the nutritional and technological properties of SBP for functional food applications. Full article
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18 pages, 3415 KiB  
Article
Study on the Modification of Dietary Fiber and Degradation of Zearalenone in Corn Germ Meal by Solid-State Fermentation with Bacillus subtilis K6
by Jiahao Li, Kailong Li, Langwen Tang, Chun Hua, Na Chen, Chenxian Yang, Ying Xin and Fusheng Chen
Foods 2025, 14(15), 2680; https://doi.org/10.3390/foods14152680 - 30 Jul 2025
Viewed by 300
Abstract
Although corn germ meal is a rich source of dietary fiber, it contains a relatively low proportion of soluble dietary fiber (SDF) and is frequently contaminated with high levels of zearalenone (ZEN). Solid-state fermentation has the dual effects of modifying dietary fiber (DF) [...] Read more.
Although corn germ meal is a rich source of dietary fiber, it contains a relatively low proportion of soluble dietary fiber (SDF) and is frequently contaminated with high levels of zearalenone (ZEN). Solid-state fermentation has the dual effects of modifying dietary fiber (DF) and degrading mycotoxins. This study optimized the solid-state fermentation process of corn germ meal using Bacillus subtilis K6 through response surface methodology (RSM) to enhance SDF yield while efficiently degrading ZEN. Results indicated that fermentation solid-to-liquid ratio and time had greater impacts on SDF yield and ZEN degradation rate than fermentation temperature. The optimal conditions were determined as temperature 36.5 °C, time 65 h, and solid-to-liquid ratio 1:0.82 (w/v). Under these conditions, the ZEN degradation rate reached 96.27 ± 0.53%, while the SDF yield increased from 9.47 ± 0.68% to 20.11 ± 1.87% (optimizing the SDF/DF ratio from 1:7 to 1:3). Scanning electron microscopy (SEM) and confocal laser scanning microscope (CLSM) revealed the structural transformation of dietary fiber from smooth to loose and porous forms. This structural modification resulted in a significant improvement in the physicochemical properties of dietary fiber, with water-holding capacity (WHC), oil-holding capacity (OHC), and water-swelling capacity (WSC) increasing by 34.8%, 16.4%, and 15.2%, respectively. Additionally, the protein and total phenolic contents increased by 23.0% and 82.61%, respectively. This research has achieved efficient detoxification and dietary fiber modification of corn germ meal, significantly enhancing the resource utilization rate of corn by-products and providing technical and theoretical support for industrial production applications. Full article
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17 pages, 661 KiB  
Article
An Ultrasonication-Assisted Green Process for Simultaneous Production of a Bioactive Compound-Rich Extract and a Multifunctional Fibrous Ingredient from Spent Coffee Grounds
by Jaquellyne B. M. D. Silva, Mayara T. P. Paiva, Henrique F. Fuzinato, Nathalia Silvestre, Marta T. Benassi and Suzana Mali
Molecules 2025, 30(15), 3117; https://doi.org/10.3390/molecules30153117 - 25 Jul 2025
Viewed by 305
Abstract
Spent coffee grounds (SCGs) are lignocellulosic residues generated from producing espresso or soluble coffee and have no commercial value. This study aimed to develop a new single-step process for extracting bioactive compounds from SCGs based on ultrasonication in an aqueous medium and simultaneously [...] Read more.
Spent coffee grounds (SCGs) are lignocellulosic residues generated from producing espresso or soluble coffee and have no commercial value. This study aimed to develop a new single-step process for extracting bioactive compounds from SCGs based on ultrasonication in an aqueous medium and simultaneously recovering the residual solid fraction, resulting in the integral utilization of the residue. This process resulted in a liquid aqueous extract (LAE) rich in bioactive compounds (caffeine: 400.1 mg/100 g; polyphenols: 800.4 mg GAE/100 g; melanoidins: 2100.2 mg/100 g) and, simultaneously, a solid multifunctional ingredient from modified spent coffee grounds (MSCGs) rich in bioactive compounds and dietary fibers (73.0 g/100 g). The liquid extract can be used as a natural ingredient for drinks or to isolate caffeine, while the solid matrix can be used to produce functional foods. This technique proved to be a promising eco-friendly alternative for the simultaneous production of two different materials from SCGs, maximizing resource efficiency, with some advantages, including short time, simplicity, and cost-effectiveness; using water as a solvent; and requiring no further purification processing. Full article
(This article belongs to the Section Green Chemistry)
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19 pages, 5242 KiB  
Article
Polydextrose Addition Improves the Chewiness and Extended Shelf-Life of Chinese Steamed Bread Through the Formation of a Sticky, Elastic Network Structure
by Chang Liu, Bing Dai, Xiaohong Luo, Hongdong Song and Xingjun Li
Gels 2025, 11(7), 545; https://doi.org/10.3390/gels11070545 - 14 Jul 2025
Viewed by 343
Abstract
This study explored the effects of adding a newly developed type of polydextrose on the appearance, sensory score, and textural parameters of steamed bread and the microstructure of dough, as well as the pasting, thermal, and thermal mechanical properties of high-gluten wheat flours. [...] Read more.
This study explored the effects of adding a newly developed type of polydextrose on the appearance, sensory score, and textural parameters of steamed bread and the microstructure of dough, as well as the pasting, thermal, and thermal mechanical properties of high-gluten wheat flours. The results revealed that, compared with a control sample, 3–10% of polydextrose addition significantly increased the hardness, adhesiveness, gumminess, and chewiness of steamed bread, but other textural parameters like springiness, cohesiveness, and resilience remained basically the same. Further, in contrast to the control sample, 3–10% polydextrose addition significantly reduced the specific volume and width/height ratio of steamed bread but increased the brightness index, yellowish color, and color difference; improved the internal structure; and maintained the other sensory parameters and total score. Polydextrose addition decreased the peak, trough, final, breakdown, and setback viscosity of the pasting of wheat flour suspension solutions but increased the pasting temperature. Polydextrose additions significantly reduced the enthalpy of gelatinization and the aging rate of flour paste but increased the peak temperature of gelatinization. A Mixolab revealed that, with increases in the amount of added polydextrose, the dough’s development time and heating rate increased, but the proteins weakened, and the peak torque of gelatinization, starch breakdown, and starch setback torque all decreased. Polydextrose additions increased the crystalline regions of starch, the interaction between proteins and starch, and the β-sheet percentage of wheat dough without yeast and of steamed bread. The amorphous regions of starch were increased in dough through adding polydextrose, but they were decreased in steamed bread. Further, 3–10%of polydextrose addition decreased the random coils, α-helixes, and β-turns in dough, but the 3–7% polydextrose addition maintained or increased these conformations in steamed bread, while 10% polydextrose decreased them. In unfermented dough, as a hydrogel, the 5–7% polydextrose addition resulted in the formation of a continuous three-dimensional network structure with certain adhesiveness and elasticity, with increases in the porosity and gas-holding capacity of the product. Moreover, the 10% polydextrose addition further increased the viscosity, freshness, and looseness of the dough, with smaller and more numerous holes and indistinct boundaries between starch granules. These results indicate that the 3–10% polydextrose addition increases the chewiness and freshness of steamed bread by improving the gluten network structure. This study will promote the addition of polydextrose in steamed bread to improve shelf-life and dietary fiber contents. Full article
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24 pages, 1159 KiB  
Review
Physicochemical and Functional Properties of Soluble and Insoluble Dietary Fibers in Whole Grains and Their Health Benefits
by Pathumi Ariyarathna, Patryk Mizera, Jarosław Walkowiak and Krzysztof Dziedzic
Foods 2025, 14(14), 2447; https://doi.org/10.3390/foods14142447 - 11 Jul 2025
Viewed by 603
Abstract
The growing global prevalence of non-communicable diseases (NCDs) is drawing an increasing amount of attention to the health-promoting potential of whole-grain dietary fibers. Whole grains are rich sources of both soluble dietary fiber (SDF) and insoluble dietary fiber (IDF), contributing distinct physicochemical properties [...] Read more.
The growing global prevalence of non-communicable diseases (NCDs) is drawing an increasing amount of attention to the health-promoting potential of whole-grain dietary fibers. Whole grains are rich sources of both soluble dietary fiber (SDF) and insoluble dietary fiber (IDF), contributing distinct physicochemical properties and playing vital roles in promoting human health. This review provides a comprehensive analysis of the dietary fiber compositions of various whole grains, including wheat, oats, barley, rye, corn, sorghum, and rice, highlighting their structural characteristics, physiochemical properties, and associated health benefits. The physicochemical properties of dietary fibers, such as solubility, water- and oil-holding capacity, viscosity, swelling ability, and bile-acid-binding capacity, contribute significantly to their technological applications and potential health benefits, particularly in the prevention of NCDs. Although there is growing evidence supporting their health benefits, global whole-grain intake remains below recommended levels. Therefore, promoting whole-grain intake and developing fiber-rich functional foods are essential for enhancing public health and preventing chronic diseases. Future research should focus on enhancing the bioavailability and functionality of whole-grain dietary fibers, optimizing the methods by which they are extracted, and exploring their potential applications in the food and pharmaceutical industries. Full article
(This article belongs to the Section Food Physics and (Bio)Chemistry)
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15 pages, 1370 KiB  
Article
Influence of Enzymatic Hydrolysis on Composition and Technological Properties of Black Currant (Ribes nigrum) Pomace
by Aurelija Kairė, Jolita Jagelavičiūtė, Loreta Bašinskienė, Michail Syrpas and Dalia Čižeikienė
Appl. Sci. 2025, 15(11), 6207; https://doi.org/10.3390/app15116207 - 31 May 2025
Viewed by 586
Abstract
Blackcurrant (Ribes nigrum) is valued for its health-promoting compounds, many of which remain in the pomace after juice extraction. Berry pomace can be considered a valuable source of dietary fiber. However, it is typically dominated by insoluble dietary fiber (IDF), and [...] Read more.
Blackcurrant (Ribes nigrum) is valued for its health-promoting compounds, many of which remain in the pomace after juice extraction. Berry pomace can be considered a valuable source of dietary fiber. However, it is typically dominated by insoluble dietary fiber (IDF), and the soluble-to-insoluble fiber ratio is often nutritionally suboptimal. The aim of this study was to evaluate the influence of enzymatic hydrolysis on the composition and technological properties of blackcurrant pomace (BCP). Three commercial enzyme preparations—Viscozyme® L, Celluclast® 1.5 L, and Pectinex® Ultra Tropical (Novozymes A/S, Denmark)—were used for enzymatic hydrolysis, which was conducted at 50 °C for 1 h. The enzymatic treatments altered BCP’s chemical composition and technological properties. Pectinex® Ultra Tropical and Viscozyme® L primarily hydrolyzed SDF, while Celluclast® 1.5 L was more effective on IDF, resulting in increased SDF content and an improved SDF/IDF ratio. Enzymatic hydrolysis reduced the oil retention capacity and impaired stabilizing properties, but it increased both the water retention capacity and the solubility index. It was found that the creaming index of the pomace deteriorated with decreased IDF content. The findings indicate that the effects of enzymatic modification on BCP’s composition and technological properties can vary significantly, supporting its potential application in the development of novel food products. Full article
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21 pages, 7434 KiB  
Article
Effects of Different Dual-Modified Jujube Juicing Residue Dietary Fibers on the Properties of Egg Protein Gels Induced by Alkalinity and Heat
by Xinyu Zheng, Ling Dang, Yichan Zhang, Xinyu Liu, Hui Wang, Yajun Zheng, Xinling Song, Zhihui Wei, Jiayao Zhang and Xiaoyang Guo
Gels 2025, 11(6), 399; https://doi.org/10.3390/gels11060399 - 27 May 2025
Viewed by 368
Abstract
Egg protein gels have relatively poor water-holding capacity, hardness, and freeze–thaw properties. Jujube juicing residue dietary fiber (JJRDF) is available, but it is rarely used in the food industry because of its poor hydration properties. Versions of JJRDF modified via cellulase and xylanase [...] Read more.
Egg protein gels have relatively poor water-holding capacity, hardness, and freeze–thaw properties. Jujube juicing residue dietary fiber (JJRDF) is available, but it is rarely used in the food industry because of its poor hydration properties. Versions of JJRDF modified via cellulase and xylanase hydrolysis separately coupled with carboxymethylation (JJRDF-CXHC), phosphate crosslinking (JJRDF-CXHPC), and acetylation (JJRDF-CXHA) were prepared, and their effects on heat-induced and alkaline-induced egg protein gels (HA-EPGs) were studied. Smaller particle sizes and higher solubility, viscosity, expansion volume, and ability to retain water were observed in JJRDF-CXHC, JJRDF-CXHPC, and JJRDF-CXHA compared to JJRDF (p < 0.05). JJRDF-CXHC showed the highest viscosity (18.46 cP) and expansion volume (10.40 mL/g). Higher random coil and β-sheet contents resulted in an increase in pH, adhesiveness, hardness, and chewiness, and a decrease in the water-losing rate in freeze–thaw cycles, and gastric digestion was observed in the HA-EPGs as a consequence of adding JJRDF, JJRDF-CXHC, JJRDF-CXHPC, and JJRDF-CXHA at 3–5 g/100 g. Moreover, JJRDF-CXHC and JJRDF-CXHPC were better at improving the textural quality of the unmodified HA-EPG compared to JJRDF-CXHA and JJRDF (p < 0.05). Therefore, to improve egg protein gel quality, JJRDF modified with cellulase and xylanase hydrolysis separately coupled with carboxymethylation and crosslinking is a good choice. However, the functionalities of these modified JJRDFs should be studied. Full article
(This article belongs to the Section Gel Analysis and Characterization)
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22 pages, 2571 KiB  
Article
Moderate Ohmic Field Modification of Okara and Its Effects on Physicochemical Properties, Structural Organization, and Functional Characteristics
by Zhongwen Cao, Chengcheng Xie, Cheng Yang, Xingyu Liu and Xiangren Meng
Foods 2025, 14(10), 1833; https://doi.org/10.3390/foods14101833 - 21 May 2025
Viewed by 412
Abstract
This study employed ohmic heating to investigate its impact on the physicochemical properties, structural organization, and functional characteristics of okara. Ohmic heating was applied with different field strengths and holding times. After moderate ohmic treatment, the water-holding capacity, oil-holding capacity, and swelling capacity [...] Read more.
This study employed ohmic heating to investigate its impact on the physicochemical properties, structural organization, and functional characteristics of okara. Ohmic heating was applied with different field strengths and holding times. After moderate ohmic treatment, the water-holding capacity, oil-holding capacity, and swelling capacity of okara increased by 51.11%, 88.89%, and 43.64%, respectively. The microstructure and secondary structure were improved. The total sugar and soluble dietary fiber content were enhanced. The levels of active substances such as total flavonoids and total phenols significantly increased, leading to improved antioxidant capacity. The properties of okara were influenced by the field strength and holding time. This study provides new insights for the processing and development of okara, particularly in the application of functional foods. Full article
(This article belongs to the Section Food Physics and (Bio)Chemistry)
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16 pages, 6610 KiB  
Article
Optimization of Solid-State Fermentation Process for Dietary Fiber in Flaxseed Meal and Analysis of Its Microstructure and Functional Properties
by Chunpeng Hou, Yiyang Zhang, Jiaxun Chen, Jianguo Hu, Chenxian Yang, Fusheng Chen, Tingwei Zhu, Ying Xin and Xiaohui Geng
Foods 2025, 14(10), 1722; https://doi.org/10.3390/foods14101722 - 13 May 2025
Cited by 1 | Viewed by 667
Abstract
Flaxseed meal (FSM) is a by-product of flaxseed product production that is wasted unreasonably at present. In this study, we used Bacillus subtilis K6, a dominant microbial strain, for solid-state fermentation (SSF) of FSM following preliminary screening to improve FSM utilization efficiency and [...] Read more.
Flaxseed meal (FSM) is a by-product of flaxseed product production that is wasted unreasonably at present. In this study, we used Bacillus subtilis K6, a dominant microbial strain, for solid-state fermentation (SSF) of FSM following preliminary screening to improve FSM utilization efficiency and enhance the soluble dietary fiber (SDF) content while modifying its functional properties. FSM’s microstructure was characterized before and after fermentation, and the functional properties of the dietary fiber (DF) in the FSM were assessed. Single-factor experiments combined with response surface methodology were conducted to optimize SSF parameters using SDF yield as the response variable. The optimal conditions were determined as follows: 45 h fermentation time, 40.5 °C temperature, and 1:0.65 material-to-liquid ratio. Under these conditions, the SDF yield reached 33.45 ± 0.24%, an SDF yield increase of 36.92%. Scanning electron microscopy and confocal laser scanning microscopy demonstrated FSM’s structural disruption during fermentation. Furthermore, SDF and insoluble DF showed improved water-holding, oil-holding, and swelling capacities following fermentation. These results indicate that SSF effectively enhances the SDF content in FSM and optimizes its functional properties, thereby providing a theoretical foundation for the valorization of flaxseed by-products. Full article
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16 pages, 713 KiB  
Article
The Influence of Integrated and Intensive Grain Production on the Content and Properties of Chemical Components in Rye Grain
by Krzysztof Buksa, Alicja Sułek and Michał Szczypek
Molecules 2025, 30(9), 1880; https://doi.org/10.3390/molecules30091880 - 23 Apr 2025
Cited by 1 | Viewed by 403
Abstract
The effect of integrated and intensive grain production technologies on the content and properties of chemical components in rye (Secale cereale L.) grain of new varieties is not known. This study aimed to examine the effect of production technology on the content [...] Read more.
The effect of integrated and intensive grain production technologies on the content and properties of chemical components in rye (Secale cereale L.) grain of new varieties is not known. This study aimed to examine the effect of production technology on the content and properties of chemical components of rye grain. Grain from four Polish rye varieties obtained as a result of integrated and intensive production was examined. In general, the use of intensive technology resulted in receiving a 7.9% higher yield of grain with a 3.7% higher content of starch, characterized by a higher share of amylose and lower molar mass compared to grain cultivated using the integrated method. Moreover, grain from intensive production contained 0.6% more water-soluble arabinoxylan of a high molar mass but a lower content of ferulic acid, compared to grain obtained by the integrated method. Rye grain from intensive production contained 0.4% more protein, 0.3% more soluble dietary fiber, and similar amounts of phytates than grain cultivated using the integrated method. Regardless of the production method, the hybrid varieties KWS Vinetto and KWS Bono had the highest grain yield and grain with a low content of protein, total and soluble dietary fiber, and extractable arabinoxylan of a high molar mass but low content of ferulic acid. Full article
(This article belongs to the Special Issue Bioactive Compounds in Food and Their Applications)
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20 pages, 1741 KiB  
Article
Productivity and Quality Characteristics of Tomato Fruits (Solanum lycopersicum) Are Improved by the Application of a Green Seaweed (Ulva ohnoi)
by Adrian Alejandro Espinosa-Antón, Rosalba Mireya Hernández-Herrera, Sandra Fabiola Velasco-Ramírez, Ana Cristina Ramírez-Anguiano and Eduardo Salcedo-Pérez
Agriculture 2025, 15(7), 750; https://doi.org/10.3390/agriculture15070750 - 31 Mar 2025
Cited by 1 | Viewed by 885
Abstract
In the last decade, interest in the use of seaweed and seaweed-derived products in horticulture has grown due to their great potential as biostimulants for increasing yields and improving food quality in multiple crops. A greenhouse experiment was conducted to investigate the effects [...] Read more.
In the last decade, interest in the use of seaweed and seaweed-derived products in horticulture has grown due to their great potential as biostimulants for increasing yields and improving food quality in multiple crops. A greenhouse experiment was conducted to investigate the effects of the application of the green seaweed Ulva ohnoi (either as a seaweed suspension [SWS] or seaweed extract [SWE]) on the yield, size, shape, and nutritional quality (i.e., proximate composition and dietary antioxidant content) of tomato fruits (Solanum lycopersicum L. cv. Rio Fuego). A total of 36 tomato plants were potted individually and organized into three experimental groups: SWS (plants drenched with 250 mL of seaweed suspension [2.0%]), SWE (plants drenched with 250 mL of seaweed extract [0.2%]), and control (plants irrigated with water). Each treatment included three replications. The fruits harvested (66%) from SWS-treated plants were produced during the earliest harvest stages. In contrast, the fruits harvested from SWE-treated plants (82%) and control plants (77%) were produced during the late and very late harvest stages. Notably, SWS application significantly enhanced the number of fruits harvested per plant, average fruit weight, yield (kg/plant), number of seeds per fruit, and fruit size. Furthermore, tomato fruits from plants treated with either SWS or SWE exhibited higher percentages of protein, fat, crude fiber, dry matter, and total soluble solids, as well as lower acidity and reduced total carbohydrate content, compared to the control. The antioxidant metabolites in tomatoes, including lycopene, β-carotene, flavonoids, and phenolic acids, increased following the application of SWS and SWE, while anthocyanin and ascorbic acid contents increased only in SWS-treated plants. These results demonstrate that both forms of U. ohnoi application have biostimulating effects on tomato. In particular, the use of SWS shows great potential as a strategy to enhance tomato fruit productivity and quality in sustainable horticultural systems. Full article
(This article belongs to the Section Crop Production)
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16 pages, 1812 KiB  
Article
Composition and Technological Properties of Modified Lingonberry (Vaccinium vitis-idaea L.) Pomace
by Simona Šimkutė, Loreta Bašinskienė, Michail Syrpas and Dalia Čižeikienė
Appl. Sci. 2025, 15(7), 3661; https://doi.org/10.3390/app15073661 - 26 Mar 2025
Cited by 1 | Viewed by 492
Abstract
Lingonberry pomace (LP) is a by-product rich in valuable bioactive compounds and can be used in the food industry after various treatments and property characterization. This study aimed to evaluate the impact of commercially available enzymes (Viscozyme® L, Pectinex® Ultra Tropical, [...] Read more.
Lingonberry pomace (LP) is a by-product rich in valuable bioactive compounds and can be used in the food industry after various treatments and property characterization. This study aimed to evaluate the impact of commercially available enzymes (Viscozyme® L, Pectinex® Ultra Tropical, and Celluclast® 1.5 L) and supercritical carbon dioxide (SFE-CO2) extraction technology on the chemical composition and technological properties of treated LP products. The Megazyme kit was used to determine the soluble dietary fiber (SDS) and insoluble dietary fiber (IDF) contents, while the changes in mono-, disaccharide, and oligosaccharides were analyzed by applying high-pressure liquid chromatography with a refractive index detector. The analyzed properties were as follows: the water swelling capacity (WSC), water retention capacity (WRC), water solubility index (WSI), oil retention capacity (ORC), bulk density (BD), and emulsion stability of modified LP. The tested LP contained 8.49 g/100 g of SDF and 65.36 g/100 g of IDF (in dry matter). The partial separation of lipophilic substances during SFE-CO2 extraction did not significantly affect the enzymatic hydrolysis efficiency. The amount of oligosaccharides in the LP increased using enzymes with pectinolytic activity (Viscozyme® L and Pectinex® Ultra Tropical), while cellulolytic enzymes (Celluclast® 1.5 L) increased the amount of SDF and improved the IDF/SDF ratio. Enzymatic hydrolysis increased the SI, WRC, and ORC of LP powder. Emulsions with LP hydrolyzed with Pectinex® Ultra Tropical demonstrated the highest stability during storage. This study demonstrates that the modification of LP powders provides diverse technological properties, which could expand the application of such products for further food production. Full article
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15 pages, 4227 KiB  
Article
Biofermentation of Wheat Bran by Monascus anka and Production of High Value-Added Dietary Fiber
by Xuefeng Wu, Siqi He, Hongyi Xu, Hui Zhang, Jing Cai, Min Zhang, Dongdong Mu, Zhenhong Li, Xingjiang Li and Lanhua Liu
Fermentation 2025, 11(3), 157; https://doi.org/10.3390/fermentation11030157 - 20 Mar 2025
Viewed by 681
Abstract
In this study, wheat bran was used to prepare dietary fiber by Monascus anka in liquid fermentation. The structural and functional characteristics of wheat bran dietary fiber were analyzed. Scanning electron microscopy and X-ray diffraction analysis indicated that the insoluble dietary fiber matrix [...] Read more.
In this study, wheat bran was used to prepare dietary fiber by Monascus anka in liquid fermentation. The structural and functional characteristics of wheat bran dietary fiber were analyzed. Scanning electron microscopy and X-ray diffraction analysis indicated that the insoluble dietary fiber matrix was disrupted during the liquid fermentation. Infrared spectroscopy and differential scanning calorimetry analysis demonstrated that intramolecular hydrogen bonds were broken and the oligosaccharides increased. The soluble dietary fiber content increased from 10.7 g/100 g to 16.5 g/100 g, which contributed to improvements in the water-holding capacity, oil-holding capacity, and swelling capacity of wheat bran dietary fiber. UV–Vis spectroscopy analysis demonstrated that the M. anka wheat bran fermentation broth (MWFB) mainly contained yellow pigments (236.6 μ mL−1). HPLC-MS spectrometry further showed MWFB contained three known Monascus pigments: monasine (observed. m/z 359.1853 [M+H]+), ankaflavin (observed. m/z 387.2151 [M+H]+), and monascorubrin (observed. m/z 382.2007 [M+H]+). In conclusion, M. anka can make the most use of wheat bran and improve the structure and function of dietary fiber, thereby expanding its application potential in functional food additives, gut microbiota modulation, and low-calorie baked goods. Full article
(This article belongs to the Special Issue Applied Microorganisms and Industrial/Food Enzymes, 2nd Edition)
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15 pages, 2393 KiB  
Article
Effects of Irrigation Water Amount and Humic Acid on β-Glucan Synthesis in Post-Anthesis Grains of Naked Oats
by Chunxiang Sun, Qi Wang, Wen Sun, Junying Wu, Shihua Gao, Yandi Liu and Baoping Zhao
Life 2025, 15(3), 343; https://doi.org/10.3390/life15030343 - 21 Feb 2025
Viewed by 663
Abstract
Naked oats offer substantial nutritional and health benefits, primarily due to their main dietary fiber component, soluble β-(1,3)(1,4)-D-glucan (β-glucan). In a pool experiment, humic acid (HA) was applied once during both the booting and anthesis stages at varying irrigation amounts (60 mm, 120 [...] Read more.
Naked oats offer substantial nutritional and health benefits, primarily due to their main dietary fiber component, soluble β-(1,3)(1,4)-D-glucan (β-glucan). In a pool experiment, humic acid (HA) was applied once during both the booting and anthesis stages at varying irrigation amounts (60 mm, 120 mm, and 180 mm) to assess changes in β-glucan content in grains post-anthesis. Results indicated that at 5 days post-anthesis (DPA), the β-glucan content (3.14% W/W) in grains increased by 16%with the application of HA, compared to the control treatment of spraying an equal volume of water (p < 0.01). The β-glucan content (4.13%, 4.51%) at 15 and 25 DPA reflects increases of 9% and 5% compared to the control. Overall, the application of HA enhanced the β-glucan content in grains, with levels gradually increasing at 5, 15, and 25 DPA; however, the amplitude of the increase gradually declined over time. The β-glucan content in grains at 5 and 15 DPA, along with glucose content in panicles at 20 DPA, directly influenced the β-glucan content in grains at 25 DPA. At 10 DPA, the distribution of sucrose in the leaves and panicles influences the soluble sugar content, subsequently regulating the β-glucan content in the grains at 15 DPA. Specifically, the sucrose content in the leaves exerts a positive regulatory effect, whereas in the panicles exerts a negative regulatory effect. Full article
(This article belongs to the Special Issue Advances in Dryland Agriculture Science)
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15 pages, 1212 KiB  
Article
Implementation of Plum Skin as a Structuring Agent in Plum Spread
by Aleksandra Bajić, Biljana Cvetković, Jasna Mastilović, Miroslav Hadnađev, Marijana Djordjević, Miljana Djordjević and Bojana Filipčev
Foods 2025, 14(4), 697; https://doi.org/10.3390/foods14040697 - 18 Feb 2025
Cited by 1 | Viewed by 853
Abstract
Plum skin, a by-product of industrial plum juice production, is rich in phenolic bioactives, functional compounds, and dietary fibers. These compounds support health, while the fibers may also act as structuring agents in food processing. This study investigated the structuring properties of lyophilized [...] Read more.
Plum skin, a by-product of industrial plum juice production, is rich in phenolic bioactives, functional compounds, and dietary fibers. These compounds support health, while the fibers may also act as structuring agents in food processing. This study investigated the structuring properties of lyophilized plum skin (LPS) in functional plum spreads produced in laboratory (F-LS) and semi-industrial (F-IS) environments, compared to a control spread (CS). Textural and rheological properties were analyzed through penetration, spreadability, flow, and dynamic oscillatory tests. Total, soluble, and insoluble dietary fibers (TDF, SDF, and IDF) in LPS and plum purée (PP) were measured using the enzymatic gravimetric method, and pectic substances contents were quantified using the colorimetric carbazole method. Fourier transform infrared spectroscopy confirmed the presence of polysaccharides and pectins in LPS. LPS had higher TDF, SDF, and IDF compared to PP, with TDF in LPS at 38.98 ± 0.52 g/100 g d.m. and IDF as the predominant fraction. The pectin content in LPS was 0.73 ± 0.03 g/100 g d.m., and water retention capacity ranged from 3.63 to 3.86 g/g depending on temperature (room, 50, and 82 °C). Incorporating LPS into the F-IS spread significantly increased all textural and rheological parameters, with TDF three times higher (6.69 g/100 g) compared to CS. All samples exhibited viscoelastic gel-like behavior, and LPS was a statistically significant structuring agent in both functional spreads compared to CS. Full article
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