Search Results (119)

Plastid division regulatory genes play a crucial role in the morphogenesis of chloroplasts and amyloplasts. Chloroplasts are the main sites for photosynthesis and metabolic reactions, while amyloplasts are the organelles responsible for forming and storing starch granules. The proper division of chloroplasts and amyloplasts is essential for plant growth and yield maintenance. Therefore, this study aimed to examine the J175 (FtsZ2-2) gene, cloned from an ethyl methanesulphonate (EMS) mutant involved in chloroplast and amyloplast division in maize, through map-based cloning. We found that J175 encodes a cell division protein, FtsZ (filamentous temperature-sensitive Z). The FtsZ family of proteins is widely distributed in plants and may be related to the division of chloroplasts and amyloplasts. The J175 protein is localized in plastids, and its gene is expressed across various tissues. From the seedling stage, the leaves of the j175 mutant exhibited white stripes, while the division of chloroplasts was inhibited, leading to a significant increase in volume and a reduction in their number. Measurement of the photosynthetic rate showed a significant decrease in the photosynthetic efficiency of j175. Additionally, the division of amyloplasts in j175 grains at different stages was impeded, resulting in irregular polygonal starch granules. RNA-seq analyses of leaves and kernels also showed that multiple genes affecting plastid division, such as FtsZ1, ARC3, ARC6, PDV1-1, PDV2, and MinE1, were significantly downregulated. This study demonstrates that the maize gene j175 is essential for maintaining the division of chloroplasts and amyloplasts and ensuring normal plant growth, and provides an important gene resource for the molecular breeding of maize. Full article

Background/Objectives: Angiogenesis is the multistep process of the formation of new blood vessels. It is beneficial in scenarios that require tissue repair and regeneration, such as wound healing, bone fracture repair, and recovery from ischemic injuries like stroke, where new blood vessel formation restores oxygen and nutrient supply to damaged areas. Extremely low-frequency electromagnetic stimulation (ELF-EMS), which involves electromagnetic fields in the frequency range of 0–300 Hz, have been shown to reduce ischemic stroke volume by improving cerebral blood flow and recovery effects that are dependent on eNOS. Based on previous results, we herein explore the effects of ELF-EMS treatment (13.5 mT/10 and 60 Hz) on the activation of angiogenic processes in vitro in homeostatic conditions. Methods: Using human microvascular endothelial cells (HMEC-1), we studied cell proliferation, migration, and tube formation in vitro, as well as nitric oxide production and the effect of calcium and nitric oxide (NO) on these processes. Moreover, blood vessel formation was studied using a chicken chorioallantoic membrane (CAM) assay. Results: Our results showed that ELF-EMS increases proliferation, tube formation, and both the migration and transmigration of these cells, the latter of which was mediated via NO. In turn, calcium inhibition decreased ELF-EMF-induced NO production. Furthermore, ELF-EMS significantly increased blood vessel formation in the CAM assay. Conclusions: Our results indicated that ELF-EMS exposure (13.5 mT/10 and 60 Hz) significantly induces angiogenesis in vitro and in ovo, underscoring its potential application in the treatment of conditions characterized by insufficient blood supply. Full article

Background: Listening to music has been widely reported to improve resistance exercise performance. However, few studies have considered lyrical content. The act of using explicit language has been shown to alter performance and psychophysiological responses to exercise. Although explicit language is widely used in mainstream music, it is unknown if altering explicit lyric content in music influences performance and psychophysiological responses to resistance exercise. Thus, the purpose of this study was to investigate the effects of censoring explicit lyrical music on bench press performance and psychophysiological responses to exercise. Methods: In a counterbalanced crossover manner, resistance-trained males (n = 11) were subjected to two conditions, namely (1) explicit music (EM) or (2) censored music (CM). Following a warm-up, music played continuously as participants completed 2 sets × 2 repetitions as explosively as possible, while a linear position transducer monitored the mean velocity of the barbell. Participants then completed 3 sets × repetitions to failure (RTFs) at 60% of a 1-repetition maximum (1-RM) separated by 2 min of rest. Motivation to exercise, psychological arousal, and rating of perceived exertion (RPE) were measured post-exercise. Total RTFs, mean velocity, motivation, psychological arousal, and RPE were compared between music conditions. Results: Findings show that total RTFs (p = 0.012) was significantly lower with CM versus EM, while mean velocity (p = 0.844) was not different between conditions. Psychological arousal (p = 0.005) and motivation (p = 0.002) were lower with CM versus EM. CM also resulted in a higher RPE (p = 0.011) compared to EM. Conclusions: Findings suggest that CM results in worse repetition volume compared to EM during resistance exercise but does not influence explosive ability. Changes in performance may be due to underlying decreases in motivation and psychological arousal. CM may also cause less dissociation, as evidenced by a higher RPE. Future research investigating the effects of lyrical content on exercise performance is warranted to further support current findings. Full article

Reducing the use of chemical inputs (fertilizers, pesticides) in agriculture while maintaining crop productivity is the main challenge facing sub-Saharan African family farming systems. The use of effective microorganisms (EM) is among the various innovative approaches for minimizing chemical inputs and the environmental impact of agricultural production and protecting soil health while enhancing crop yields and improving food security. This study sought to characterize the microbial biodiversity of local beneficial microorganisms (BMs) products from locally fermented forest litter and investigate their ability to enhance tomato plant growth and development. Beneficial microorganisms (BMs) were obtained by anaerobic fermentation of forest litter collected in four agroecological regions of Senegal mixed with sugarcane molasses and various types of carbon sources (groundnut shells, millet stovers, and rice bran in different proportions). The microbial community composition was analyzed using next-generation rDNA sequencing, and their effects on tomato growth traits were tested in greenhouse experiments. Results show that regardless of the litter geographical collection site, the dominant bacterial taxa in the BMs belonged to the phyla Firmicutes (27.75–97.06%) and Proteobacteria (2.93–72.24%). Within these groups, the most prevalent classes were Bacilli (14.41–89.82%), α-proteobacteria (2.83–72.09%), and Clostridia (0.024–13.34%). Key genera included Lactobacillus (13–65.83%), Acetobacter (8.91–72.09%), Sporolactobacillus (1.40–43.35%), and Clostridium (0.08–13.34%). Fungal taxa were dominated by the classes Leotiomycetes and Sordariomycetes, with a prevalence of the acidophilic genus Acidea. Although microbial diversity is relatively uniform across samples, the relative abundance of microbial taxa is influenced by the litter’s origin. This is illustrated by the PCoA analysis, which clusters microbial communities based on their litter source. Greenhouse experiments revealed that five BMs (DK-M, DK-G, DK-GM, NB-R, and NB-M) significantly (p < 0.05) enhanced tomato growth traits, including plant height (+10.75% for DK-G and +9.44% for NB-R), root length (+56.84–62.20%), root volume (+84.32–97.35%), root surface area (+53.16–56.72%), and both fresh and dry shoot biomass when compared to untreated controls. This study revealed that forest-fermented litter products (BMs), produced using litter collected from various regions in Senegal, contain beneficial microorganisms known as plant growth-promoting microorganisms (PGPMs), which enhanced tomato growth. These findings highlight the potential of locally produced BMs as an agroecological alternative to inorganic inputs, particularly within Senegal’s family farming systems. Full article

The sodium/proton exchanger NHA2, also known as SLC9B2, is important for insulin secretion, renal blood pressure regulation, and electrolyte retention. Recent structures of bison NHA2 has revealed its unique 14-transmembrane helix architecture, which is different from SLC9A/NHE members made up from 13-TM helices. Sodium/proton exchangers are functional homodimers, and the additional N-terminal helix in NHA2 was found to alter homodimer assembly. Here, we present the cryo-electron microscopy structures of apo human NHA2 in complex with a Fab fragment and also with the inhibitor phloretin bound at 2.8 and 2.9 Å resolution, respectively. We show how phosphatidic acid (PA) lipids bind to the homodimer interface of NHA2 on the extracellular side, which we propose has a regulatory role linked to cell volume regulation. The ion binding site of human NHA2 has a salt bridge interaction between the ion binding aspartate D278 and R432, an interaction previously broken in the bison NHA2 structure, and these differences suggest a possible ion coupling mechanism. Lastly, the human NHA2 structure in complex with phloretin offers a template for structure-guided drug design, potentially leading to the development of more selective and potent NHA2 inhibitors. Full article

This paper introduces a novel strategy for an intelligent plug-in hybrid electric vehicle (PHEV) energy optimization strategy based on machine learning (ML) prediction of the upcoming journey, without recourse to navigation or other external data, which underpins many of the existing approaches. This study, based on extended real-world data (journeys history from 10 vehicles over 12 months), shows that trip patterns can be learnt quite effectively using classic ML classification algorithms. In particular, the RusBoosted ensemble classifier performed consistently well across the heterogeneous dataset (volume of data for training and variable imbalance in the datasets, reflecting the natural variability in the vehicle usage profiles), providing sufficiently accurate predictions for the proposed EMS strategy. Performance evaluation experiments were carried out using a model-in-the-loop (MIL) simulation set-up developed in this research. The results demonstrated that the proposed strategy has the potential to deliver significant reductions in engine running time (up to 76% on routine short journeys), with associated benefits in CO₂ consumption and tailpipe emissions, as well as enhanced engine reliability. The broader importance of this study is that it demonstrates the great potential of using predictive insights from computation-efficient and robust ML to learn vehicle usage patterns to optimize the control strategies without reliance on uncertain external inputs. Full article

Background/Objectives: Evidence supports the benefits of concurrent training (CT), which combines endurance and resistance exercises, for enhancing health and physical fitness. Recently, low-volume, time-efficient exercise approaches such as low-volume high-intensity interval training (LOW-HIIT), whole-body electromyostimulation (WB-EMS), and single-set resistance training (1-RT) have gained popularity for their feasibility and efficacy in improving various health outcomes. This study investigated the effects of low-volume CT, focusing on (1) whether exercise order affects cardiometabolic health, inflammation, and fitness adaptations and (2) which combination, LOW-HIIT plus WB-EMS or LOW-HIIT plus 1-RT, yields better results. Methods: Ninety-three obese metabolic syndrome (MetS) patients undergoing caloric restriction were randomly assigned to four groups performing the different low-volume CT protocols over 12 weeks. Outcomes included cardiometabolic, inflammatory, and fitness parameters. Results: In both combinations, no significant differences were found regarding exercise order. However, the pooled LOW-HIIT and 1-RT group achieved superior improvements in blood pressure, blood lipids, inflammation markers (CRP, hsCRP), the MetS severity score, and overall fitness compared to the LOW-HIIT and WB-EMS combination. Compared to previous studies using these modalities individually, LOW-HIIT plus 1-RT appeared to further reduce inflammation, whereas LOW-HIIT combined with WB-EMS was less effective for cardiometabolic health, potentially due to interference effects between modalities. Conclusions: While LOW-HIIT plus WB-EMS appears to be a viable option for individuals unable to perform traditional resistance training, the findings suggest prioritizing LOW-HIIT plus 1-RT to maximize health outcomes. These findings highlight the importance of tailored exercise prescriptions and the need for further research into optimizing CT protocols for diverse populations. Full article

Predicting soil–water dynamics in Moistube irrigation (ΜΤΙ) favours understanding ΜΤΙ functioning mechanisms and technical parameter design. This study proposed a physically based infiltration (PH) model extending the Green–Ampt (GA) model to a two-dimensional polar coordinate system. We treated Moistube as a clay and considered the infiltration from internal Moistube to surrounding soils. The performances of the PH model, together with a semi-physical–empirical (PH–EM) model and a numerical simulation (NUM) model, were evaluated based on regulated working pressure head (WPH) experiments. A HYDRUS 2D model was used based on experimental design to reproduce the soil–water dynamics by assigning Moistube and soil two sets of hydraulic parameters. WPH increase or decrease treatments were applied to Moistube. The Moistube discharge rate, infiltration volume, and wetting front (WF) advance were analyzed and predicted by three models. The results showed that cumulative infiltration, Moistube discharge, and effective saturation around Moistube were enhanced or abated under WPH increase or decrease, with WF accelerating or decelerating. The modelled effective saturation varied between 0.45 and 0.70, providing suitable moist conditions for crops. Percentage of bias (PBIAS) and mean absolute percentage relative error (MAPRE) were employed to evaluate model performances. Three models well-predicted infiltration characteristics and WF advance but differed in accuracy. The PH model overestimated and underestimated the Moistube discharge rate in early and later phases. The prediction accuracy in WF varied across infiltration phases and WPH modes. The PH–EM model yielded accurate results due to its empirical attribute. The NUM model produced novel phenomena of infiltration characteristics at WPH adjustment points, i.e., the discharge rate exponentially decreased over time after the WPH increased but presented restraining followed by rebounding trends after the WPH decreased. The NUM model strongly depended on the selection of the Moistube hydraulic parameters. Extending the GA model to a two-dimensional polar coordinate system by treating Moistube as a clay was practicable in modelling soil water dynamics, thereby contributing to designing and optimizing MTI technical indexes. Full article

RNA viruses, being submicroscopic organisms, have intriguing biological makeups and substantially impact human health. Microscopic methods have been utilized for studying RNA viruses at a variety of scales. In order of observation scale from large to small, fluorescence microscopy, cryo-soft X-ray tomography (cryo-SXT), serial cryo-focused ion beam/scanning electron microscopy (cryo-FIB/SEM) volume imaging, cryo-electron tomography (cryo-ET), and cryo-electron microscopy (cryo-EM) single-particle analysis (SPA) have been employed, enabling researchers to explore the intricate world of RNA viruses, their ultrastructure, dynamics, and interactions with host cells. These methods evolve to be combined to achieve a wide resolution range from atomic to sub-nano resolutions, making correlative microscopy an emerging trend. The developments in microscopic methods provide multi-fold and spatial information, advancing our understanding of viral infections and providing critical tools for developing novel antiviral strategies and rapid responses to emerging viral threats. Full article

Background: The timely management of rapidly evolving epidemiological scenarios caused by disease outbreaks is crucial to prevent devastating consequences. However, delayed laboratory diagnostics can hamper swift health policy and epidemic response, especially in remote regions such as the western Brazilian Amazon. The aim of the article is to analyze the impact of the COVID-19 pandemic on the volume and characteristics of emergency medical services (EMS) in Manaus, focusing on how the pandemic affected sensitive indicators such as response time and the use of advanced life support ambulances. Additionally, the study seeks to understand how changes in prehospital EMS patterns, triggered by the pandemic, could be utilized as health surveillance tools, enabling a more rapid response in epidemic scenarios. Methods: This retrospective, descriptive study included data from the SAMU (Serviço de Atendimento Móvel de Urgência) medical records between January and June 2020. Results: A total of 45,581 calls resulted in mobile units being dispatched during this period. These patients were predominantly male (28,227, 61.9%), with a median age of 47 years (IQR 30–67). The median response time significantly increased during the pandemic, reaching a median of 45.9 min (IQR 30.6–67.7) (p < 0.001). EMS calls were reduced for trauma patients and increased for other medical emergencies, especially respiratory conditions, concomitantly to an escalation in the number of deaths caused by SARS and COVID-19 (p < 0.001). The employment of advanced life support ambulances was higher during the pandemic phase (p = 0.0007). Conclusion: The COVID-19 pandemic resulted in a temporary disorder in the volume and reason for EMS calls in Manaus. Consequently, sensitive indicators like the response time and the employment of advanced life support ambulances were negatively affected. Sudden prehospital EMS pattern changes could play an important role in health surveillance systems, allowing for earlier establishment of countermeasures in epidemics. The impact of the COVID-19 pandemic on prehospital EMS and its role in health surveillance should be further explored. Full article

Background/Objectives: Cardiovascular involvement in severe cirrhosis presents diagnostic challenges and carries significant prognostic implications. This study aims to evaluate the relationship between liver disease severity and portal hypertension with the burden of diastolic dysfunction. Methods: We prospectively enrolled patients with hepatic cirrhosis, classified according to the Child–Pugh criteria. Of the 102 patients included, 65 were classified as Group A (non-severe cirrhosis: Child–Pugh Classes A and B) and 37 as Group B (severe cirrhosis: Child–Pugh Class C). Portal vein and spleen diameters were assessed using abdominal ultrasound. All patients underwent echocardiographic evaluation. LV systolic function was assessed by measuring ejection fraction, while diastolic function was evaluated using three parameters: E/Em ratio, E/Vp ratio, and indexed left atrial volume. Results: We observed a significantly greater burden of diastolic dysfunction in Group B compared to Group A. Specifically, the E/Vp ratio was 2.2 ± 0.4 in Group B versus 1.9 ± 0.3 in Group A (p < 0.001); the indexed LA volume was 34.5 ± 3.2 mL/m² in Group B versus 30.1 ± 2.9 mL/m² in Group A (p < 0.001); and the E/Em ratio was 17.0 ± 3.0 in Group B versus 11.5 ± 2.8 in Group A (p < 0.001). Additionally, the mean diameters of the portal vein and spleen were larger in Group B, with measurements of 14.3 ± 2.1 mm versus 11.5 ± 1.6 mm for the portal vein and 15.0 ± 1.2 mm versus 11.7 ± 1.5 mm for the spleen (p < 0.001), which correlated with the extent of diastolic dysfunction. Conclusions: Diastolic dysfunction was prevalent in 55% of patients with liver cirrhosis. The burden of diastolic dysfunction was higher in patients with severe hepatic cirrhosis compared to those with milder forms, and it correlated with the severity of portal hypertension, as assessed by measuring portal vein diameter and spleen diameter. Full article

Phosphorus (P) deficiency is one of the main reasons limiting plant production of Brassica napus L. Exploring the dynamics of leaf intracellular substances and the correlations with photosynthesis and growth helps to understand the response mechanisms of B. napus L. to P deficiency. This study conducted experiments on B. napus L. plants by measuring the leaf electrophysiological parameters, leaf structure, elastic modulus (E_m), photosynthesis, and growth indices under different P treatment conditions. The dynamics of leaf intracellular water and nutrients of B. napus L. were calculated and analyzed by using the electrophysiological parameters, and the plant tolerance threshold to low-P stress was discovered. The results indicated that the status of the leaf intracellular water and nutrients remained stable when the P concentration was not lower than 0.250 mmol·L⁻¹, but maximized the photosynthesis and growth at a P level of 0.250 mmol·L⁻¹. The 0.125 mmol·L⁻¹ P concentration significantly decreased the mesophyll cell volume, and the palisade–sponge ratio and tightness degree of leaf tissue structure were remarkably increased. This led to an increase in cell elastic modulus, and significantly improved the water retention capacity of leaf cells. At the same time, the intracellular water use efficiency and total nutrient transport capacity of leaves remained stable. As a result, the photosynthesis and growth of plants were maintained at the same level as that of the control group. However, photosynthesis and growth were clearly inhibited with a further decrease in P concentration. Therefore, 0.125 mmol·L⁻¹ was the tolerance threshold of B. napus L. to low P. With the help of electrophysiological information, the effects of the dynamics of intracellular substances on photosynthesis and growth of B. napus L. under low-P stress can be investigated, and the plant’s adaptive response can be revealed. However, the findings of the current hydroponic study are not directly applicable to field conditions with naturally P-deficient soils. Full article

This study explored the changes in the rate of torque development scaling factor (RTD-SF) and maximum voluntary isometric contraction (MVC) variables following six weeks of unilateral isometric electromyostimulation (EMS) and voluntary (VOL) exercises. Twenty-six physically active participants were randomly assigned to EMS (n = 13) or a VOL group. MVC and RTD-SF of the quadriceps femoris of both legs were assessed before and after training. EMS and VOL exercises had identical frequency (three sessions/week), intensity (60% MVC), volume (40 contractions), and work-to-rest ratio (18 min: 6.25 s of work/20 s of rest). There were no between-group differences for the trained leg with overall increases in maximal torque (Tmax) of ~29% (d = 2.11–2.12), ~13% for RTDmax (d = 0.92–1.10); ~23% for Intercept (d = 0.72–0.78), and reduction in RTD-SF by ~15% (d = 1.01–1.10). In the non-trained leg, significant moderate change was only observed after EMS for RTD-SF which decreased by 12.5% (d = 0.76). Both EMS and VOL training applied at equivalent workloads positively impact on Tmax, RTDmax, and Intercept, but they negatively affect the quickness with which muscle contracts across a wide range of submaximal forces. Using a moderate training intensity in regularly physically active participants could explain the absence of cross-education in the VOL group. Full article

Entrustable Professional Activities-based (EPAs-based) assessments gained much interest among clinical educators, especially after the AAMC publication in 2014. In 2016, a standardized tool, the National Clinical Assessment Tool for Medical Students in Emergency Medicine (NCAT-EM), was developed at a national conference. Since 2018, the modified NCAT-EM has been used at Central Michigan University School of Medicine at shift ends, midway through clerkships, and upon completion of the clerkship. This empirical study analyzed student performance progression in order to enhance school assessment practices and inform future action plans. Descriptive and inferential statistics were calculated. During the 2021–2022 academic year, 89 faculty and residents assessed 97 students on 238 submission days. The students generally received multiple sets of daily shift feedback. Two domains, note writing and practice-based learning, showed significant differences between the mid-clerkship formative and summative scoring. Professionalism issues were evident with this cohort of students. The study provides some validity evidence regarding student performance and progression within the context. The NCAT-EM provides values and benefits as evidenced by the substantial volume of assessor feedback during its fourth year of implementation. This study provides directions for future action plans for faculty training, promising continuous improvement in emergency medicine assessment practices. Full article

RNA-based therapeutics, including siRNA, have obtained recognition in recent years due to their potential to treat various chronic and rare diseases. However, there are still limitations to lipid-based drug delivery systems in the clinical use of RNA therapeutics due to the need for optimization in the design and the preparation process. In this study, we propose adaptive focused ultrasound (AFU) as a drug loading technique to protect RNA from degradation by encapsulating small RNA in nanoliposomes, which we term nanoplexes. The AFU method is non-invasive and isothermal, as nanoplexes are produced without direct contact with any external materials while maintaining precise temperature control according to the desired settings. The controllability of sample treatments can be effectively modulated, allowing for a wide range of ultrasound intensities to be applied. Importantly, the absence of co-solvents in the process eliminates the need for additional substances, thereby minimizing the potential for cross-contaminations. Since AFU is a non-invasive method, the entire process can be conducted under sterile conditions. A minimal volume (300 μL) is required for this process, and the treatment is speedy (10 min in this study). Our in vitro experiments with silencer CD44 siRNA, which performs as a model therapeutic drug in different mammalian cell lines, showed encouraging results (knockdown > 80%). To quantify gene silencing efficacy, we employed quantitative polymerase chain reaction (qPCR). Additionally, cryo-electron microscopy (cryo-EM) and atomic force microscopy (AFM) techniques were employed to capture images of nanoplexes. These images revealed the presence of individual nanoparticles measuring approximately 100–200 nm in contrast with the random distribution of clustered complexes observed in ultrasound-untreated samples of liposome nanoparticles and siRNA. AFU holds great potential as a standardized liposome processing and loading method because its process is fast, sterile, and does not require additional solvents. Full article