Maturation of urease involves post-translational insertion of nickel ions to form an active site with a carbamylated lysine ligand and is assisted by urease accessory proteins UreD, UreE, UreF and UreG. Here, we review our current understandings on h...
Enzyme-induced carbonate precipitation has been studied for wind erosion control in arid areas. A comparative study was conducted between the pure urease- and crude soybean urease-induced carbonate precipitation methods with the same enzyme activity...
Urease has attracted much attention, as it is directly involved in the formation of infection stones and contributes to the pathogenesis of urolithiasis, pyelonephritis, ammonia and hepatic encephalopathy, hepatic coma and urinary catheter encrustati...
Helicobacter pylori (H. pylori) produces urease in order to improve its settlement and growth in the human gastric epithelium. Urease inhibitors likely represent potentially powerful therapeutics for treating H. pylori; however, their instability and...
The stability (longevity of activity) of three crude urease extracts was evaluated in a laboratory study as part of an effort to reduce the cost of urease for applications that do not require high purity enzyme. A low-cost, stable source of urease wi...
Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide andammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes thehydrolysis of urea only. This feature is a basic diagnostic criterion used in thedeterm...
Urease, a metalloenzyme widely present in various organisms, catalyzes the hydrolysis of urea to ammonia and CO2 and has been extensively utilized in studies and applications of microbially induced calcium carbonate precipitation (MICP). While microb...
Inhibition of the urease activity of ruminal microbiota is not only beneficial for increasing dietary and endogenic urea-N utilization efficiency in ruminants but also might be applicable for the preservation of nitrogen fertilizer in soil and treatm...
Regulation of microbial urease activity plays a crucial role in improving the utilization efficiency of urea and reducing nitrogen emissions to the environment for ruminant animals. Dealing with the diversity of microbial urease and identifying highl...
Urease is a metalloenzyme that catalyzes the hydrolysis of urea, and its modulation has an important role in both the agricultural and medical industry. Even though numerous molecules have been tested against ureases of different species, their clini...
A novel method for the rapid modification of fullerene for subsequent enzyme attachment to create a potentiometric biosensor is presented. Urease was immobilized onto the modified fullerene nanomaterial. The modified fullerene-immobilized urease (C60...
Urease is a therapeutic target associated with several important diseases and health problems. Based on our previous work on the inhibition of glucosidase and other enzymes and exploiting the privileged structure assigned to the (thio)barbiturate (py...
Urea granule dissolution in soil and soil urease activity are essential parameters for the accurate prediction of nitrogen dynamics after urea application, but both are scarcely studied. The response of urease activity to temperature is unclear under...
Furan chalcone scaffolds belong to the most privileged and promising oxygen-containing heterocyclic class of compounds, which have a wide spectrum of therapeutic applications in the field of pharmaceutics, pharmacology, and medicinal chemistry. This...
Urease is a nickel-containing enzyme that is essential for the survival of several and often deadly pathogenic bacterial strains, including Helicobacter pylori. Notwithstanding several attempts, the development of direct urease inhibitors without sid...
The urease enzyme has been an important target for the discovery of effective pharmacological and agricultural products. Thirteen regio-selectively alkylated benzimidazole-2-thione derivatives have been designed to carry the essential features of ure...
Inhibition of ruminal microbial urease is of particular interest due to its crucial role in regulating urea-N utilization efficiency and nitrogen pollution in the livestock industry. Acetohydroxamic acid (AHA) is currently the only commercially avail...
In this work, highly fluorescent gold nanowire arrays (Au NWs) are successfully synthesized by assembling Zn2+ ions and non-emissive oligomeric gold-thiolate clusters using mercaptopropionic acid both as a reducing agent and a growth ligand. The synt...
Urease is a nickel-dependent amidohydrolase that catalyses the decomposition of urea into carbamate and ammonia, a reaction that constitutes an important source of nitrogen for bacteria, fungi and plants. It is recognized as a potential antimicrobial...
The development of new anti-ureolytic compounds is of great interest due to the newly discovered role of urease inhibitors in crop protection. Purine degradation and the generation of ammonium by urease are required for the full virulence of biotroph...
Soil bacteria can produce urease, which catalyzes the hydrolysis of urea to ammonia (NH3) and carbamate. A variety of urease inhibitors have been proposed to reduce NH3 volatilization by interfering with the urease activity. We report a quantum mecha...
Urease-producing (ureolytic) microbes have given rise to environmental and public health concerns because they are thought to contribute to emissions of ammonia and to be a virulence factor for infections. Therefore, it is highly important to have th...
The effect of pH on the supramolecular structure of Helicobacter pylori urease was studied by means of molecular dynamics simulations at seven different pHs. Appropriate urease charge distributions were calculated using a semi-grand canonical Monte C...
Dental restorative materials suffer from major drawbacks, namely fracture and shrinkage, which result in failure and require restoration and replacement. There are different methods to address these issues, such as increasing the filler load or chang...
The large amount of ammonia released during agricultural application of urea fertilizer can result in a partial loss of applied nitrogen, having a detrimental effect on air quality. Although Chamomile recutita has nitrogen transformation inhibitory p...
Numerous studies have addressed the utilization of glutaraldehyde (GA) as a homobifunctional cross-linker. However, its applicability has been impeded due to several issues, including the tendency of GA molecules to undergo polymerization. Herein, a...
There is growing interest for bioanalytical tools that might be designed for a specific user, primarily for research purposes. In this perspective, a new, highly stable potentiometric sensor based on glassy carbon/polyazulene/NH4+-selective membrane...
Alzheimer’s disease (AD) causes dementia and memory loss in the elderly. Deposits of beta-amyloid peptide and hyperphosphorylated tau protein are present in a brain with AD. A filtrate of Helicobacter pylori’s culture was previously found...
Urease, a pivotal enzyme in nitrogen metabolism, plays a crucial role in various microorganisms, including the pathogenic Helicobacter pylori. Inhibiting urease activity offers a promising approach to combating infections and associated ailments, suc...
Stomach infection with Helicobacter pylori (H. pylori) causes severe gastroduodenal diseases in a large number of patients worldwide. The H. pylori infection breaks up in early childhood, persists lifelong if not treated, and is associated with chron...
Urease is an enzyme that plays a significant role in the hydrolysis of urea into carbonic acid and ammonia via the carbamic acid formation. The resultant increase in pH leads to the onset of various pathologies such as gastric cancer, urolithiasis, h...
Chronic Helicobacter pylori infection increases the risk of gastric cancer and induction of hypoxia-induced factor (HIF), which is frequently associated with the development and progression of several types of cancer. We recently showed that H. pylor...
Weathered coal is known to have potential inhibitory effects on urease activity, thus reducing the loss of nitrogen from fertilizer such as ammonia. This means that it can be used as a urea enhancer to promote urea utilization efficiency. However, th...
Different parts of Acacia nilotica (L.) Delile, Calotropis procera (Aiton) W.T. Aiton, Adhatoda vasica Nees, Fagoniaar abica L. and Casuarina equisetifolia L. are traditionally used in folk medicine for the treatment of a variety of common ailments l...
Climate change poses several challenges in the wine industry, including increasing risks related to chemical food contaminants such as biogenic amines and ethyl carbamate (EC). In this work, we focused on urea removal in red wines by immobilized acid...
Encouraging advances have been made in the application of microbial mineralization towards fixing and improving desertified sandy soils. However, desert soils in arid areas exhibit high salinity that may limit urease activity and production in microb...
Compost amendments are widely recognized as an effective strategy for improving soil quality, modulating enzyme activities, and enhancing nitrogen cycling. Urease, a key enzyme in nitrogen transformation, is characterized by kinetic parameters such a...
18 January 2019
Cadmium (Cd) is a highly toxic metal that can affect human health and environmental safety. The purpose of this study was to research the removal of Cd from an environmental perspective. In this article, four highly urease-active strains (CZW-2, CZW-...
This study examines the chemical composition, antioxidant properties, and urease inhibitory effects of Hyoscyamus muticus L. subsp. falezlez (Coss.) Maire. Using LC-ESI-MS/MS, 19 distinct phenolic compounds were identified, with chlorogenic acid bein...
Ammonia (NH3) emissions have become a serious environmental pollution problem, and livestock production is an important source of NH3 emissions, especially pig farming. The origin of NH3 release is the hydrolysis of urea in urine that is catalyzed by...
After evaluating the ecological and economic aspects, it is predicted that the use of urease inhibitors and biological preparations should reduce the risk of nutrient leaching by using fertilizers containing amide, ammonium, and nitrate forms of nitr...
The fluoroquinolone antibacterial drug ciprofloxacin (cip) has been used to cap metallic (silver and gold) nanoparticles by a robust one pot synthetic method under optimized conditions, using NaBH4 as a mild reducing agent. Metallic nanoparticles (MN...
Infection by Proteus mirabilis causes urinary stones and catheter incrustation due to ammonia formed by urease (PMU), one of its virulence factors. Non-enzymatic properties, such as pro-inflammatory and neurotoxic activities, were previously reported...
Designing and developing small organic molecules for use as urease inhibitors is challenging due to the need for ecosystem sustainability and the requirement to prevent health risks related to the human stomach and urinary tract. Moreover, imaging an...
Optimizing nitrogen (N) availability to plants is crucial for achieving maximum crop yield and quality. However, ensuring the appropriate supply of N to crops is challenging due to the various pathways through which N can be lost, such as ammonia (NH...
Urease and nitrification inhibitors represent ways to reduce nitrogen losses; their presence modifies microbial and enzymatic activity in the soil, affecting pH and organic matter (OM), which in turn affects the mobility of heavy metals. To evaluate...
Enzyme-induced carbonate precipitation (EICP) is a new biogeotechnical ground improvement technique that uses calcium carbonate (CaCO3) formed by biochemical processes to increase soil strength and stiffness. In this paper, crude urease extracted fro...
The field experiment was performed in 2019–2021 at the Experimental Station of Vytautas Magnus University Agriculture Academy (54°52′ N, 23°49′ E). The soil of the experimental field was Endohipogleyic-Eutric Planasol. The s...
This article describes the design and synthesis of a series of novel amantadine-thiourea conjugates (3a–j) as Jack bean urease inhibitors. The synthesized hybrids were assayed for their in vitro urease inhibition. Accordingly, N-(adamantan-1-yl...
Ureolytic microbes in soil produce urease to catalyze the hydrolysis of urea to NH3/NH4+. Manure is widely applied in agriculture and has the potential to influence soil urease activity. In this study, we examined the responses of the ureolytic micro...
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