Search Results (846)

The topito pepper (Capsicum chinense) is a tropical fruit of economic and gastronomic importance in the Caribbean region, valued for its nutritional content. However, this fruit is susceptible to postharvest fungal diseases, including those caused by the phytopathogenic fungus Penicillium expansum, which can degrade fruit quality and pose a health risk due to the potential presence of mycotoxins such as patulin. In this context, we evaluated the protective effects of coatings with chitosan (CS), clove essential oil (CEO), and their combination (CS+CEO) on sweet peppers stored at 12 °C for 12 days after harvest. The results indicate that the film-forming solution exhibited an acidic pH (5.33–5.44), a density of ~1.0 g/cm³, and viscosities ranging from 2.75 to 32.9 cP. Furthermore, the results indicate that coatings with CS and CS+CEO significantly reduced weight loss, preserved firmness (19.12–30.40 N), and delayed ripening. At the same time, the coatings exhibited inhibitory effects on P. expansum and aerobic mesophiles. The CS+CEO combination demonstrated the greatest inhibitory effect, indicating that it is a sustainable and effective strategy for the postharvest preservation of sweet peppers, thereby enhancing their value, preservation, and food security in the Caribbean region. Full article

Polygalacturonase (PG) is a key enzyme in cell wall metabolism and fruit ripening. Atemoya (Annona cherimola Mill. × A. squamosa L.) is a high-value tropical fruit that undergoes rapid postharvest softening at room temperature. However, the role of the atemoya PG gene family in this process remains unknown. This study determined that storing atemoya at 28 °C significantly reduced fruit firmness and the total pectin content but increased water-soluble pectin (WSP) and PG activity compared to storage at 15 °C. Genome-wide identification of the AaPG gene family in atemoya revealed that 40 AaPG genes were unevenly distributed across seven chromosomes. Nineteen genes were located within six tandem duplication clusters. AaPG proteins exhibited clade-specific differences: Clades B-E contained the polysaccharide lyase family 6 (PL-6) superfamily domain, while Clade A harbored the Aspergillus niger polygalacturonase 1 (Pgu1) domain and lacked several conserved motifs. Expression profiling and reverse transcription quantitative polymerase chain reaction (RT-qPCR) showed that AaPG19, AaPG21, AaPG23 and AaPG24 were specifically induced at 28 °C. Subcellular localization confirmed that these four proteins were located on the plasma membrane. These findings provide insights into the evolution and temperature-dependent regulation of the AaPG family, identifying candidate genes responsible for the rapid softening of atemoya fruit. Full article

Vitex madiensis Oliv. (Lamiaceae) is a species growing in tropical and subtropical regions throughout the world. In several African countries, the different organs of this plant, leaves, fruits, stem bark and roots are used in folk medicine for the treatment of headaches, toothaches, aches and pains. In this study, we investigated the phytochemical profile of Vitex madiensis leaf extracts using LC-MS. The antioxidant and anti-inflammatory potential of crude extracts, fractions, and pure molecules was evaluated using reactive oxygen species (ROSs) production assays and cyclooxygenase-2 inhibition assays. A bio-guided fractionation was carried out to identify the most active fractions and resulted in the isolation of four phytoecdysteroids from the n-butanol fraction: 20-hydroxyecdysone, ajugasterone C, vitexirone, and pterosterone. 20-hydroxyecdysone showed very good anti-inflammatory properties with a significant reduction of more than 70% of COX-2 expression in induced LPS-stimulated human blood leukocytes compared to the control. This study confirmed the therapeutic potential of phytoecdysteroids. Full article

Bactrocera correcta is an important quarantine pest of mango, and the development of phytosanitary treatments that achieve quarantine security without compromising fruit quality remains a major challenge in fresh-fruit trade. Heat treatment is a residue-free phytosanitary option, but the temperatures required to control fruit flies often approach the tolerance limits of tropical fruit, leaving a narrow margin between quarantine security and commodity injury. In this study, a phosphine (PH₃)-assisted forced hot-air treatment was evaluated for the phytosanitary disinfestation of B. correcta in mango fruit. The developmental progression of B. correcta in mango fruit was characterized, the heat tolerance of different developmental stages was compared, and the efficacy of PH₃ followed by forced hot-air treatment (PH₃→Heat) against eggs was quantified using probit time–mortality analysis. Large-scale confirmatory validation and postharvest quality assessment were then conducted. Eggs were identified as the most heat-tolerant stage. PH₃ pre-fumigation significantly enhanced forced hot-air treatment, with 0.7 g m⁻³ PH₃ providing the most practical improvement at quarantine-relevant endpoints. According to this schedule, LT_99.9968 was reduced by 44 min for heat treatment alone, from 269.0 to 224.5 min, and the large-scale validation yielded no survivors. Postharvest quality evaluation showed that PH₃→Heat did not adversely affect firmness, total soluble solids, or titratable acidity during shelf life. These results demonstrate that PH₃-assisted forced hot-air treatment is a technically feasible and commercially promising phytosanitary strategy for mango fruit. Full article

Tamarindus indica L., a key economic tree species in tropical regions, suffers severely from postharvest decay. From 2023 to 2025, disease fruits exhibiting pericarp softening, pulp browning, and sticky exudates were collected in Yunnan, China. Pathogenicity tests following Koch’s postulates, combined with morphological characterization and phylogenetic analyses of the internal transcribed spacer (ITS), translation elongation factor 1-alpha (TEF 1α), and beta-tubulin (TUB) gene regions, identified the causal pathogen as Botryosphaeria fabicerciana (isolates ZWML-06, ZWML-44, ZWML-17). This is the first report of this postharvest disease on tamarind in Yunnan, filling an etiological gap. Additionally, an endophytic bacterium, designated BV-1, was isolated from asymptomatic pulp tissues. Whole-genome sequencing and phylogenetic analysis identified it as Bacillus velezensis. Strain BV-1 exhibited strong in vitro antagonistic activity against the pathogen, indicating promising biocontrol potential. Functional annotation revealed that BV-1 possesses a complex genetic system with developed transporter systems; its core metabolic network is dominated by nitrogen metabolism and redox processes, suggesting a potential “multi-target” antimicrobial mechanism. This study provides a theoretical basis and novel resources for the green control of postharvest diseases in tamarind. Full article

The dataset presented in this manuscript consists of physicochemical, nutritional, and functional characteristics of mamey purees from three West-Indian accessions selected for their processability, i.e., Galion, Ti Jacques, and Sonson. Physicochemical analysis comprised measurements of soluble solid contents, titratable acidity, pH, and color. Proximate analysis corresponded to measurements of humidity, protein, ashes, lipid, and dietary fiber contents, and the calculation of sugar contents and energy value. Biochemical analysis consisted of measurements of polyphenols, flavonoids, carotenoids, and ascorbic acid contents. Antioxidant capacity is also reported by DPPH and ORAC assays, respectively. Raw data and mean values with standard deviations are provided for each characteristic. The data were generated to describe the quality of these mamey purees as an intermediate agrifood industry product. Full article

The composition, differences, and metabolic mechanisms of phenolic compounds in fruits and peels of three different varieties (including Clausena lansium ‘Jixin’, ‘Seedless’ and ‘Bingtang’) were investigated. The results showed that the total phenolic content in the fruits followed the order CLB (2080.14 mg/kg) > CLS > CLJ, while in peels it followed CLSp (2457.56 mg/kg) > CLJp > CLBp. Significant differences were observed in flavanols, flavonols, lignans, and phenolic acids among samples. A total of 12 (in fruits) and 9 (in peels) differential phenolic compounds (VIP > 1) were screened. Compared with CLB, several antioxidants and antibacterial phenolics were significantly up-regulated in CLS and CLJ. Pathway analysis revealed that fruit differences were mainly enriched in phenylpropanoid biosynthesis and flavone/flavonol biosynthesis pathways, whereas peel differences were mainly enriched in flavonoid biosynthesis and flavone/flavonol biosynthesis pathways. These findings provided a theoretical basis for wampee variety identification, improvement, and functional evaluation. Full article

Background/Objectives: The high temperatures associated with climate change represent an important constraint for tomato production in tropical regions, affecting plant growth, reproductive development, and fruit metabolic composition. In this context, protected cultivation systems capable of modifying greenhouse microclimates may help reduce thermal stress and maintain crop productivity. Methods: This study evaluated the effects of two protective environments, diffuse agricultural film (AF) and twin-walled polycarbonate panels with laminar water flow (P), on the agronomic performance and fruit metabolic traits of five grape–tomato hybrids grown under tropical conditions. Microclimatic variables, vegetative growth, yield components, postharvest behavior, and fruit quality attributes were evaluated, with emphasis on carotenoid accumulation. Results: Compared with the agricultural film environment, the polycarbonate system reduced global radiation and photosynthetically active radiation (PAR) and was associated with an increase in yield of approximately 25%, an increase in fruit number of approximately 13%, and an 8% increase in fruit diameter. In addition, some hybrids cultivated under the polycarbonate system showed greater lycopene and β-carotene accumulation, indicating that microclimate moderation may favor carotenoid-related fruit quality depending on genotype. Principal component analysis revealed a clear separation between cultivation environments, with the polycarbonate system more closely associated with yield-related and canopy development traits, whereas the agricultural film environment was linked to biomass accumulation and selected physicochemical attributes. Among the evaluated hybrids, BS IGR0104, Jacy, and GI7545 showed the most favorable combination of agronomic performance and fruit quality traits. Conclusions: These results demonstrate the importance of climate-adaptive protected cultivation systems and hybrid selection for improving tomato productivity under tropical heat conditions. Full article

Manilkara zapota (M. zapota), commonly known as sapodilla, is a tropical fruit recognized for its nutritional value and diverse phytochemical composition. This review critically summarizes recent evidence (2013–2026) regarding the phytochemistry, biological activities, safety profile, and food industry relevance of M. zapota, using literature retrieved from Google Scholar, PubMed, Scopus, Web of Science, Science Direct, and other scientific databases. Different parts of the plant, including its fruits, leaves, seeds, and bark, contain a wide range of bioactive compounds, such as gallic acid, caffeic acid, quercetin, catechin, myricetin, kaempferol, β-sitosterol, stigmasterol, alkaloids, tannins, saponins, triterpenes, and glycosides. Experimental studies have demonstrated antioxidant, anti-inflammatory, antimicrobial, gastroprotective, glucose homeostasis, and antiproliferative activities associated with these phytochemicals. Mechanistically, M. zapota extracts have been reported to modulate oxidative stress markers, inflammatory mediators, apoptotic pathways, and lipid metabolism-related enzymes in in vitro and animal studies. Available toxicological evidence suggests that certain extracts were well tolerated under specific experimental conditions; however, further standardized safety assessments and clinical investigations remain necessary. In addition to its pharmacological relevance, M. zapota has potential applications in functional foods and food processing industries, including jams, jellies, spreads, fruit bars, beverages, and nutraceutical formulations. Overall, M. zapota represents a promising underutilized plant with potential relevance for food, nutraceutical, and future biomedical applications. Full article

Vapor Pressure Deficit (VPD) is a critical determinant of atmospheric evaporative demand and plant water stress in tropical agricultural systems. This study applied a Gaussian Mixture Model (GMM) and K-Means clustering to 36,528 hourly meteorological observations collected from Eastern Thailand between August 2021 and September 2025, with the objective of identifying distinct atmospheric moisture regimes relevant to precision irrigation management in durian cultivation. Two input configurations were evaluated: a multivariate feature space comprising air temperature, relative humidity, wind speed, solar radiation, and VPD; and a univariate input consisting of VPD alone. Model selection for GMM was guided by the Akaike Information Criterion (AIC) and Bayesian Information Criterion (BIC), while K-Means performance was assessed using the Elbow method, Silhouette Coefficient, Calinski–Harabasz Index, and Davies–Bouldin Index. For the multivariate input, GMM identified K = 7 as the optimal number of clusters, supported by the largest single-step reduction in both AIC and BIC at this transition point. For the univariate VPD input, K = 5 was selected as the most parsimonious and agriculturally interpretable solution. The seven clusters derived from the multivariate GMM were organized into four atmospheric moisture regimes, such as very low, moderate, high, and very high evaporative demand, capturing the full spectrum of diurnal and seasonal VPD variability characteristic of Eastern Thailand. The results demonstrate that GMM-based probabilistic clustering applied to multivariate meteorological inputs provides a more comprehensive characterization of atmospheric moisture dynamics than univariate or geometric clustering approaches, offering a practical framework for tiered irrigation scheduling and drought stress early warning systems in tropical fruit cultivation. Full article

Potato (Solanum tuberosum) is a vital global non-cereal food crop severely threatened by bacterial wilt, caused by Ralstonia solanacearum(R. solanacearum). Conventional diagnostics like PCR and ELISA, though effective, are destructive and time-consuming, limiting large-scale field applications. This study investigates hyperspectral imaging (HSI) as a non-invasive, rapid, and accurate alternative for early detection and severity grading of potato bacterial wilt. Using a portable HSI system (400–1000 nm), spectral data were collected from inoculated potato plants (‘Longshu No. 7’) at 0, 24, 48, and 72 h post-inoculation, alongside disease severity assessment (grades 0–4). After comprehensive spectral preprocessing and feature band extraction via Competitivse Adaptive Reweighted Sampling (CARS), we developed two distinct sets of models: one for early detection (temporal classification) using Partial Least Squares-Discriminant Analysis (PLS-DA) and Principal Component Analysis-Linear Discriminant Analysis (PCA-LDA), and another for severity grading. The SNV + SG + MC + PLS-DA model achieved exceptional accuracy, exceeding 97% for early detection, while the MSC + SG + MC + CARS + PLS-DA model yielded >97% accuracy for severity grading. These results were supported by low misclassification rates in confusion matrices. This work establishes a robust HSI-based framework for high-throughput screening of resistant potato germplasm and advances precision agriculture strategies for bacterial wilt management. Full article

Accurate hourly crop evapotranspiration (ETc) estimation is important for data-driven irrigation management support in tropical horticulture, yet existing approaches are constrained by data requirements and an inability to capture multi-scale temporal dynamics. This study proposes a Transformer encoder model for one-step-ahead hourly FAO-56 PM-derived ETc estimation in a durian orchard in Chanthaburi Province, Eastern Thailand, using 36,528 hourly meteorological observations obtained from the Visual Crossing Weather API for the orchard location over four years, with ETc computed from these inputs using the FAO-56 Penman–Monteith equation. The model employs a 168-h (7-day) look-back window, three stacked encoder blocks with multi-head self-attention ($h=8$, $d_{\mathrm{model}}=128$), and five meteorological input features (air temperature, relative humidity, solar radiation, wind speed, and ETc). A SARIMA$(2,1,2){(1,0,0)}_{24}$ model trained on the same dataset served as the statistical baseline. The Transformer achieved an RMSE of 0.0308 mm/h, MAE of 0.0188 mm/h, and $R^2$ of 0.9018 on the 168-h test set, outperforming SARIMA (RMSE = 0.0717, MAE = 0.0593, $R^2$ = 0.4688), representing a 57.0% reduction in RMSE, a 68.3% reduction in MAE, and a 92.4% improvement in $R^2$. The Transformer also achieved a daytime-only RMSE of 0.0414 mm/h vs. 0.0791 mm/h for SARIMA, and a daily cumulative ETc MAE of 0.1599 mm/day vs. 0.5901 mm/day, demonstrating superior accuracy during agronomically critical periods. The Transformer accurately reproduced both the 24-h diurnal cycle and the 7-day weekly pattern of ETc, whereas SARIMA exhibited a damped amplitude response. A recursive 168-h heuristic simulation demonstrated that the model generates physically plausible ETc patterns under an approximated meteorological scenario, suggesting the approach warrants further investigation as a component of future irrigation decision-support research. These results highlight the potential of Transformer-based deep learning for site-specific, proof-of-concept ETc estimation from meteorological inputs in tropical fruit production, pending validation across diverse sites and seasons. Full article

The use of underutilized biomass improves resource-use efficiency and reduces agricultural waste, particularly in temperate systems cultivating tropical crops. Papaya (Carica papaya L.), grown as an annual crop in these systems, produces substantial trunk biomass that is typically discarded after harvest. This study evaluated the potential of papaya trunk xylem rays as an edible resource through compositional, sensory, and functional analyses. Trunks were harvested at the end of the fruiting period (December) and after exposure to a cold wave (January) and were classified by organ types and maturity level. Xylem rays showed moisture and carbohydrate contents comparable to those of green papaya fruit, and were judged as edible by all panelists (100%) in December-harvested samples. However, exposure to a cold wave reduced sweetness and increased bitterness, resulting in decreased overall acceptability. Nevertheless, boiling effectively reduced bitterness and improved palatability even in cold-exposed samples. In addition, xylem rays exhibited higher total polyphenol content than green papaya fruit, while showing comparable DPPH radical scavenging activity. These results suggest that xylem rays have potential as an edible plant resource with antioxidant-related properties, contributing to resource-use efficiency and potentially providing opportunities for biomass valorization in temperate production systems. Full article

Self-incompatibility (SI) is an important plant mechanism that prevents inbreeding depression by recognizing and rejecting self-pollen, thereby promoting outcrossing. However, SI can also act as a barrier in breeding programs, presenting significant challenges to breeders. Passion fruit (Passiflora edulis), a tropical fruit species of substantial economic importance, also serves as a valuable system for investigating SI mechanisms within the Passifloraceae. Nevertheless, the molecular basis of SI in passion fruit has not yet been elucidated. In this study, we investigated the SI system in yellow passion fruit (P. edulis f. flavicarpa) and employed transcriptomic analysis to examine the time-course transcriptional responses following different pollination treatments. Transcriptomic analysis revealed distinct gene expression dynamics under different pollination treatments: self-pollinated samples exhibited stronger and earlier transcriptional changes, whereas the number of differentially expressed genes (DEGs) in cross-pollinated samples was relatively lower. Numerous pathways previously associated with sporophytic self-incompatibility (SSI) were enriched in the stigma samples after self-pollination. Reactive oxygen species (ROS) are crucial signaling molecules involved in pollen germination and pollen tube growth during SI responses. Our results showed that ROS-related pathways were enriched in stigma tissues after self-pollination. In addition, oxidative stress-related responses were detected in the style shortly after self-pollination, suggesting that plastid-associated or general oxidative stress processes may also be involved, although the precise source of ROS requires further validation. FERONIA, ROP9, and ARC1 are key genes related to the SI system in Brassica. In the passion fruit SI response, the expression levels of these genes increased in the style, indicating a spatial expression pattern different from that reported in classical Brassicaceae SSI systems. Together with cytological observations showing that self-pollen rejection occurs at the stigma surface, our results suggest that yellow passion fruit may employ an SSI-like regulatory framework while exhibiting a lineage-specific spatial deployment of SI-related regulators. Overall, this study provides new transcriptomic insights into the SI mechanism of yellow passion fruit, establishes a molecular framework for understanding SI in P. edulis f. flavicarpa, and offers novel insights into the diversity of plant SI systems. Full article

Extending the fruiting season of Annona squamosa L. requires overcoming autumn and winter flowering declines. This study investigates the efficacy of light-quality regulation technologies and their temperature dependence for floral induction. Field surveys initially identified temperature as the primary climatic factor governing flowering. Under suboptimal autumn temperatures, red light (R-660) night-break (NB) treatments significantly enhanced shoot growth and flowering compared to other light spectra. Transcriptomic analysis revealed 2027 upregulated and 341 downregulated transcripts consistently regulated by R-660, with significant enrichment in the plant hormone signal transduction pathway. Furthermore, R-660 upregulated cold response genes (e.g., CBFs, WRKYs, ERD7), which are associated with the maintenance of vegetative vigor under suboptimal autumn temperatures. However, mid-winter R-660 NB failed to induce flowering without supplemental greenhouse heating. Ultimately, warm ambient temperature is the absolute prerequisite for A. squamosa floral induction, with R-660 serving as a highly effective seasonal supplement to extend autumn flowering. Full article