Search Results (31)

PYL proteins are core components of the abscisic acid (ABA) signaling pathway and are involved in plant responses to biotic and abiotic stresses. In this study, a total of 19, four, and eight PYL genes were identified in Saccharum spontaneum, the Saccharum spp. hybrid R570, and Sorghum bicolor, respectively. Phylogenetic analysis classified these PYL genes into three distinct groups. Cis-acting element analysis, Gene Ontology annotation, and Kyoto Encyclopedia of Genes and Genomes pathway enrichment and gene expression profile indicated that members of the PYL gene family are mainly associated with hormone signaling and stress-related biological processes. The ScPYL8 gene (GenBank accession number: OR838856) was isolated from sugarcane cultivar QT3. Expression of the ScPYL8 gene was induced under stresses of cold, PEG, SA, MeJA, ABA, and brown stripe disease (Bipolaris setariae). The gene was expressed in roots, stems and leaves, with the highest expression level in leaves. Subcellular localization analysis showed that the ScPYL8 protein localized to the cytoplasm and nucleus. ScPYL8 overexpression in tobacco activated the reactive oxygen species defense system and regulated the ABA and jasmonic acid signaling pathways, enhancing its resistance against Fusarium solani var. coeruleum. These findings provide insights into the expression, function, and evolutionary characteristics of the PYL gene family in sugarcane, offering valuable genetic resources for future molecular breeding. Full article

Sugarcane (Saccharum spp.) is a globally important crop for sugar and bioenergy production. However, genetic improvement through conventional breeding is constrained by long breeding cycles, low genetic gain, and considerable operational complexity arising from its highly allopolyploid and aneuploid genome. With the increasing global demand for sustainable food and renewable energy, sugarcane breeding programs must accelerate the development of high-yielding, stress-tolerant cultivars through the integration of advanced biotechnological tools with traditional breeding approaches. Recent advances in genetic engineering, genomic selection (GS), and high-throughput omics technologies, including genomics, transcriptomics, proteomics, metabolomics, and phenomics, have substantially enhanced the efficiency of trait improvement related to growth, development, yield, and stress resilience. The integration of multi-omics data enables the dissection of regulatory networks linking genotype to phenotype, improves predictive accuracy, and provides deeper insights into the molecular mechanisms underlying complex traits. These integrative approaches support more informed selection decisions and accelerate genetic gain in sugarcane breeding programs. This review synthesizes recent technological developments and their practical applications in sugarcane improvement. It highlights the strategic implementation of transgenic and genome-editing technologies, genomic selection, and multi-omics integration to enhance yield potential and resistance to biotic and abiotic stresses, thereby contributing to sustainable sugarcane production and global food and bioenergy security. Full article

The cold-regulated (Cor413) gene family encodes plant-specific, multispanning transmembrane proteins that localize to the plasma and thylakoid membranes; these genes are regulated by environmental stimuli. In this study, the Cor413-1 gene, isolated from the drought and saline-tolerant wild species Saccharum spontaneum, was engineered into the elite sugarcane cultivar Co 86032 to produce a commercially superior cultivar with improved abiotic stress tolerance. Expression analysis of the Cor413-1 gene transgenic lines under drought and salinity stress exhibited distinct gene expression patterns. During stress conditions, transgenic events, such as Cor413-9 and Cor413-3, showed notable resilience to salt stress and had a high relative expression of the Cor413-1 gene and other stress-related genes. The evaluation of physiological parameters showed that under stress conditions, transgenic events experienced milder wilting and less cell membrane injury than the non-transgenic control. Transgenic lines also demonstrated elevated relative water content and better photosynthetic efficiency, with events like Cor413-10 and Cor413-12 showing exceptional performance. Biochemical analyses indicated elevated proline content, higher activity of enzymatic antioxidants such as sodium dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and a low level of malondialdehyde MDA production in the transgenic lines. Thus, demonstrating the potential of the Cor413-1 gene for developing multiple stress-tolerant cultivars. Full article

In this study, the mechanical and structural properties of biocomposites fabricated using transgenic sugarcane bagasse overexpressing sucrose synthesis were investigated. The bagasse fibers were extracted from the transgenic and non-transgenic (NT) sugarcane stalk, then treated with alkalization and carbonization, and their chemical composition was analyzed. The treated fibers were reinforced to produce biocomposites, and their mechanical and structural properties were evaluated by measuring tensile strength, elongation at break, modulus of elasticity and scanning electron microscopy. The cellulose content ranged from 40.6–44.2% in transgenic sugarcane and was higher than in NT sugarcane, with the highest content observed in transgenic SPS3. However, the cellulose and hemicellulose contents were reduced, and the lignin content was significantly increased after carbonization treatment. Alkalization treatment significantly increased the tensile strength, with the highest value of 30.46 MPa obtained at 9% NaOH concentration in a biocomposite fabricated from transgenic SPS3 bagasse fibers. However, carbonization of the SPS3 bagasse fibers lowered tensile strength and slightly increased modulus of elasticity in the biocomposite. Morphological analyses showed roughened fiber surfaces after alkalization and the formation of voids in the carbonized composites. These results indicate the potential of the transgenic sugarcane bagasse fibers with high cellulose content as a renewable reinforcement material for biocomposites. Full article

Polyphenol-Rich Sugarcane Extract (PRSE), derived from Saccharum officinarum, demonstrates significant neuroprotective effects against amyloid-beta (Aβ_1–42)-induced deficits associated with Alzheimer’s disease (AD). This study utilized transgenic C. elegans expressing Aβ_1–42 to investigate PRSE’s impact on lifespan, sensory behavior, learning, memory, and amyloid fibril accumulation. Supplementation with 5 mg/mL of PRSE extended the mean lifespan of Aβ_1–42 worms by 11% (17.78 ± 0.36 days) and reduced amyloid fibril levels by 34% in aged worms compared to untreated worms. PRSE also improved sensory behavior, with a 27% increase in naïve chemotaxis at day 8. Memory deficits were mitigated, with PRSE-treated worms showing 21% and 30% reductions in short-term associative memory loss after 1 h intervals on days 8 and 12, respectively. These improvements can be associated with the polyphenolic compounds in PRSE, which aid in reducing amyloid aggregation. The findings highlight PRSE’s potential as a dietary supplement to address AD-related symptoms and pathologies. Further studies are needed to understand its mechanisms and confirm its effectiveness in mammals, supporting its potential use as a natural preventative supplement for Alzheimer’s and related neurodegenerative diseases. Full article

Sugarcane smut caused by Sporisorium scitamineum represents the most destructive disease in the sugarcane industry, causing host hormone disruption and producing a black whip-like sorus in the apex of the stalk. In this study, the gibberellin metabolic pathway was found to respond to S. scitamineum infection, and the contents of bioactive gibberellins were significantly reduced in the leaves of diseased plants. The gibberellin receptor gene ScGID1 was identified and significantly downregulated. ScGID1 localized in both the nucleus and cytoplasm and had the highest expression level in the leaves. Eight proteins that interact with ScGID1 were screened out using a yeast two-hybrid assay. Novel DELLA proteins named ScGAI1a and ScGA20ox2, key enzymes in GA biosynthesis, were both found to interact with ScGID1 in a gibberellin-independent manner. Transcription factor trapping with a yeast one-hybrid system identified 50 proteins that interacted with the promoter of ScGID1, among which ScS1FA and ScPLATZ inhibited ScGID1 transcription, while ScGDSL promoted transcription. Overexpression of ScGID1 in transgenic Nicotiana benthamiana plants could increase plant height and promote flowering. These results not only contribute to improving our understanding of the metabolic regulatory network of sugarcane gibberellin but also expand our knowledge of the interaction between sugarcane and pathogens. Full article

Water deficit is the main limiting factor constraining sugarcane productivity, and its impact is expected to increase due to climate changes. During prolonged drought periods, most plants become extremely vulnerable to ROS accumulation, which can severely damage their photosynthetic apparatus. The PetC gene, encoding a Rieske FeS protein (ISP), has been shown to regulate the electron transport chain and protect photosystems (PSs) under drought conditions in some plant species. In sugarcane, transcriptome analysis revealed that ScPetC is repressed during drought stress in the field. In this study, we have overexpressed ScPetC in tobacco plants and evaluated its role in water-deficit tolerance. Our results indicate that the ScPetC protein structure is conserved when compared to other species. ScPetC overexpression reduced the negative impact of water deficit on plant development. This effect was associated with a positive impact on ScPetC quantum efficiency and the electron transport rate of PSII, the photosynthetic rate, and water use efficiency. The total chlorophyll content under water deficit was higher in plants overexpressing ScPetC, and this was correlated with less chlorophyll degradation from oxidative damage. Together, these results demonstrate that ScPetC confers tolerance to water deficit and oxidative stresses, making it a candidate gene for crop improvement. Full article

Sugarcane, a vital cash crop, contributes significantly to the world’s sugar supply and raw materials for biofuel production, playing a significant role in the global sugar industry. However, sustainable productivity is severely hampered by biotic and abiotic stressors. Genetic engineering has been used to transfer useful genes into sugarcane plants to improve desirable traits and has emerged as a basic and applied research method to maintain growth and productivity under different adverse environmental conditions. However, the use of transgenic approaches remains contentious and requires rigorous experimental methods to address biosafety challenges. Clustered regularly interspaced short palindromic repeat (CRISPR) mediated genome editing technology is growing rapidly and may revolutionize sugarcane production. This review aims to explore innovative genetic engineering techniques and their successful application in developing sugarcane cultivars with enhanced resistance to biotic and abiotic stresses to produce superior sugarcane cultivars. Full article

Sugarcane (Saccharum spp.), a major cash crop that is an important source of sugar and bioethanol, is strongly influenced by the impacts of biotic and abiotic stresses. The intricate polyploid and aneuploid genome of sugarcane has shown various limits for conventional breeding strategies. Nonetheless, biotechnological engineering currently offers the best chance of introducing commercially significant agronomic features. In this study, an efficient Agrobacterium-mediated transformation system that uses the herbicide-resistant CP4-EPSPS gene as a selection marker was developed. Notably, all of the plants that were identified by PCR as transformants showed significant herbicide resistance. Additionally, this transformation protocol also highlighted: (i) the high yield of transgenic lines from calli (each gram of calli generated six transgenic lines); (ii) improved selection; and (iii) a higher transformation efficiency. This protocol provides a reliable tool for a routine procedure for the generation of resilient sugarcane plants. Full article

Sugarcane is a significant primitive source of sugar and energy worldwide. The progress in enhancing the sugar content in sugarcane cultivars remains limited due to an insufficient understanding of specific genes related to sucrose production. The present investigation examined the enzyme activities, levels of reducing and non-reducing sugars, and transcript expression using RT-qPCR to assess the gene expression associated with sucrose metabolism in a high-sucrose sugarcane clone (GXB9) in comparison to a low-sucrose sister clone (B9). Sucrose phosphate synthase (SPS), sucrose phosphate phosphatase (SPP), sucrose synthase (SuSy), cell wall invertase (CWI), soluble acid invertase (SAI), and neutral invertase (NI) are essential enzymes involved in sucrose metabolism in sugarcane. The activities of these enzymes were comparatively quantified and analyzed in immature and maturing internodes of the high- and low-sucrose clones. The results showed that the higher-sucrose-accumulating clone had greater sucrose concentrations than the low-sucrose-accumulating clone; however, maturing internodes had higher sucrose levels than immature internodes in both clones. Hexose concentrations were higher in immature internodes than in maturing internodes for both clones. The SPS and SPP enzymes activities were higher in the high-sucrose-storing clone than in the low-sucrose clone. SuSy activity was higher in the low-sucrose clone than in the high-sucrose clone; further, the degree of SuSy activity was higher in immature internodes than in maturing internodes for both clones. The SPS gene expression was considerably higher in mature internodes of the high-sucrose clones than the low-sucrose clone. Conversely, the SuSy gene exhibited up-regulated expression in the low-sucrose clone. The enhanced expression of SPS in the high-sucrose clone compared to the low-sucrose clone suggests that SPS plays a major role in the increased accumulation of sucrose. These findings provide the opportunity to improve sugarcane cultivars by regulating the activity of genes related to sucrose metabolism using transgenic techniques. Full article

Phosphorus deficiency highly interferes with plant growth and development. Plants respond to persistent P deficiency by coordinating the expression of genes involved in the alleviation of stress. Promoters of phosphate transporter genes are a great choice for the development of genetically modified plants with enhanced phosphate uptake abilities, which improve crop yields in phosphate-deficient soils. In our previous study, the sugarcane phosphate transporter PHT1;2 gene showed a significantly high expression under salinity stress. In this study, the Erianthus arundinaceus EaPHT1;2 gene was isolated and characterized using various in silico tools. The deduced 542 amino acid residues have 10 transmembrane domains, with a molecular weight and isoelectric point of 58.9 kDa and 9.80, respectively. They displayed 71–96% similarity with Arabidopsis thaliana, Zea mays, and the Saccharum hybrid. To elucidate the function of the 5′ regulatory region, the 1.1 kb promoter was isolated and validated in tobacco transgenics under Pi stress. The EaPHT1;2 promoter activity was detected using a β-glucuronidase (GUS) assay. The EaPHT1;2 promoter showed 3- to 4.2-fold higher expression than the most widely used CaMV35S promoter. The 5′ deletion analysis with and without 5′ UTRs revealed a small-sized 374 bp fragment with the highest promoter activity among 5′ truncated fragments, which was 2.7 and 4.2 times higher than the well-used CaMV35S promoter under normal and Pi deprivation conditions, respectively. The strong and short promoter of EaPHT1;2 with 374 bp showed significant expression in low-Pi-stress conditions and it could be a valuable source for the development of stress-tolerant transgenic crops. Full article

Sugarcane is a complex polyploid aneuploid cash crop, and transgenic varieties are important for molecular genetic and traditional breeding approaches. Herein, the sugarcane variety ROC22 served as the receptor, the Bar gene served as a screening marker, and positive and negative fragments of the ScD27.2 gene, upstream of strigolactones (SLs) biosynthesis genes driven by the 35S promoter, were introduced by Agrobacterium tumefaciens-mediated transformation. Regenerated plants were obtained by co-culture, screening culture, and differentiation induction, and 27 sense and antisense ScD27.2 transgenic seedlings were obtained by herbicide screening. PCR detection and 1% Basta (Glufosinate) application on leaves revealed Bar in all lines, with all testing positive for herbicide application and 23 containing the target gene (positive resistance screening rate = 87.5%). q-PCR and phenotypic analyses showed that ScD27.2 expression, plant height, tiller number, root length, stem diameter, and fresh weight were decreased in transgenic (ScD27.2R-9) compared with non-transgenic (NT and ScD27.2F-2) lines. ScD27.2 expression was downregulated, and growth potential was inhibited. Under 20% PEG treatment, malondialdehyde (MDA) content in ScD27.2R-9 was higher than in NT, while proline content was lower. Under drought stress, ScD27.2 expression, MDA levels, and proline content in ScD27.2F-2 and NT were higher than in non-treated controls, ScD27.2 expression increased with time, and MDA and proline levels also increased. ScD27.2 expression in ScD27.2R-9 decreased under 20% PEG treatment, MDA and proline increased (but not to NT levels), and growth was lower than NT. The 20% PEG treatment also increased the levels of (±)-2′-epi-5-deoxystrigol and (+)-abscisic acid in the rooting culture media of ScD27.2F-2, ScD27.2R-9, and NT lines, but the levels of (+)-abscisic acid content in ScD27.2R-9 was lower than in NT. Thus, interfering with ScD27.2 expression decreased resistance to 20% PEG treatment. ScD27.2 encodes a β-carotene isomerase involved in SLs biosynthesis that might function in sugarcane resistance to drought stress. It explains the role of SLs in sugarcane growth and development and responses to drought stress. Full article

Sugarcane (Saccharum spp.) is one of the most valuable food and industrial crops. Its production is constrained due to major biotic (fungi, bacteria, viruses and insect pests) and abiotic (drought, salt, cold/heat, water logging and heavy metals) stresses. The ever-increasing demand for sugar and biofuel and the rise of new pest and disease variants call for the use of innovative technologies to speed up the sugarcane genetic improvement process. Developing new cultivars through conventional breeding techniques requires much time and resources. The advent of CRISPR/Cas genome editing technology enables the creation of new cultivars with improved resistance/tolerance to various biotic and abiotic stresses. The presence of genome editing cassette inside the genome of genome-edited plants hinders commercial exploitation due to regulatory issues. However, this limitation can be overcome by using transgene-free genome editing techniques. Transgene-free genome editing approaches, such as delivery of the RNPs through biolistics or protoplast fusion, virus-induced genome editing (VIGE), transient expression of CRISPR/Cas reagents through Agrobacterium-mediated transformation and other approaches, are discussed. A well-established PCR-based assay and advanced screening systems such as visual marker system and Transgene killer CRISPR system (TKC) rapidly identify transgene-free genome edits. These advancements in CRISPR/Cas technology speed up the creation of genome-edited climate-smart cultivars that combat various biotic and abiotic stresses and produce good yields under ever-changing conditions. Full article

Sugarcane smut caused by Sporisorium scitamineum is one of the most devastating sugarcane diseases. Furthermore, Rhizoctonia solani causes severe diseases in various crops including rice, tomato, potato, sugar beet, tobacco, and torenia. However, effective disease-resistant genes against these pathogens have not been identified in target crops. Therefore, the transgenic approach can be used since conventional cross-breeding is not applicable. Herein, the overexpression of BROAD-SPECTRUM RESISTANCE 1 (BSR1), a rice receptor-like cytoplasmic kinase, was conducted in sugarcane, tomato and torenia. BSR1-overexpressing tomatoes exhibited resistance to the bacteria Pseudomonas syringae pv. tomato DC3000 and the fungus R. solani, whereas BSR1-overexpressing torenia showed resistance to R. solani in the growth room. Additionally, BSR1 overexpression conferred resistance to sugarcane smut in the greenhouse. These three BSR1-overexpressing crops exhibited normal growth and morphologies except in the case of exceedingly high levels of overexpression. These results indicate that BSR1 overexpression is a simple and effective tool for conferring broad-spectrum disease resistance to many crops. Full article

In China, nitrogen (N) fertilizer is excessively used in sugarcane planting areas, while the nitrogen use efficiency (NUE) of sugarcane is relatively low. Mining and identifying the key genes in response to low N stress in sugarcane can provide useful gene elements and a theoretical basis for developing sugarcane varieties with high NUE. In our study, RNA-Seq combined with qRT-PCR analysis revealed that the ScAMT1.1 gene responded positively to low N stress, resulting in the stronger low N tolerance and high NUE ability of sugarcane cultivar ROC22. Then, ScAMT1.1 was cloned from sugarcane. The full-length cDNA of the ScAMT1.1 gene is 1868 bp, containing a 1491 bp open reading frame (ORF), and encoding 496 amino acids. ScAMT1.1 belongs to the AMT superfamily and shares 91.57% homologies with AMT1.1 from Oryza sativa. Furthermore, it was stably overexpressed in rice (O. sativa). Under low N treatment, the plant height and the fresh weight of the ScAMT1.1-overexpressed transgenic rice were 36.48% and 51.55% higher than that of the wild-type, respectively. Both the activity of ammonium assimilation key enzymes GS and GDH, and the expression level of ammonium assimilation key genes, including GS1.1, GS1.2, GDH, Fd-GOGAT, and NADH-GOGAT2 in the transgenic plants, were significantly higher than that of the wild-type. The grain number and grain yield per plant in the transgenic rice were 6.44% and 9.52% higher than that of the wild-type in the pot experiments, respectively. Taken together, the sugarcane ScAMT1.1 gene has the potential to improve ammonium assimilation ability and the yield of transgenic rice under low N fertilizer conditions. This study provided an important functional gene for improving sugarcane varieties with high NUE. Full article