Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges
Abstract
:1. Introduction
2. Biotic Stresses Affecting Sugarcane
2.1. Major Diseases
2.2. Major Insect Pests
3. Abiotic Stress in Sugarcane
3.1. Drought
3.2. Salt
3.3. Cold and Heat
3.4. Waterlogging
3.5. Heavy Metals
4. Transgene-Free Genome Editing: Approaches and Applications
4.1. Virus-Induced Genome Editing
4.2. Particle Bombardment/Biolistics
4.3. Agrobacterium-Mediated Transformation
4.4. Protoplast Transformation
4.5. piggyBac-Mediated Transgenesis
4.6. Grafting and Mobile RNA-Mediated Genome Editing
5. Screening of Transgene-Free GE Lines
6. Candidate Genes for Genome Editing in Sugarcane for Biotic and Abiotic Stress—Lessons from Crop Plants
7. Regulatory Issues in Commercializing GE Plants
8. Challenges in Transgene-Free Genome Editing in Sugarcane
9. Conclusions and Future Prospects
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Stress | Candidate Genes | References |
---|---|---|
Drought | SoACLA-1 | [150] |
Drought | SoP5CS | [151] |
Drought | miRNAs | [152,153] |
Drought | ScLoX, Dehydrin. Dirigent-jacalin | [154] |
Salinity | miRNAs | [155] |
Salinity | ShPHT | [156] |
Drought and salinity | SodERF3 | [157] |
Drought and salinity | SoMYB18 | [158] |
Drought and salinity | Scdr2 | [159] |
Drought, salinity and oxidative | Scdr1 | [160] |
Drought, salinity and oxidative | ScDir | [161,162] |
Drought, salinity and oxidative | ScAPX6 | [163] |
Salinity and low temperature | SspNIP2 | [164] |
Drought, salinity and cold | ShGPCR1 | [165] |
Whip smut | ScCAT1 | [166] |
Rust | Bru1 | [167] |
Stress | Crop | Target Gene | References |
---|---|---|---|
Drought | Rice | OsERA1 | [171] |
OsDST | [172] | ||
OsPYL9 | [173] | ||
OsWRKY5 | [174] | ||
Tomato | SlGID1 | [175] | |
SlMAPK3 | [176] | ||
Wheat | TaSal1 | [177] | |
Sal1 | [178] | ||
Soybean | GmHdz4 | [179] | |
Chickpea | 4CL and RVE7 | [180] | |
Salinity | Rice | OsbHLH024 | [181] |
OsRR22 | [182] | ||
OsmiR535 | [183] | ||
Tomato | SlARF4 | [184] | |
SlHyPRP1 | [185] | ||
Soybean | GmAITR | [186] | |
Heavy metals | Rice | OsNRAMP5 | [187] |
OsNRAMP1 | [188] | ||
OsARM1 | [189] | ||
Fungus | |||
Rice Blast | Rice | OsERF922 | [190] |
Rice | OsSEC3A | [191] | |
Rice | Bsr-d1, Pi21 and ERF922 | [192] | |
Powdery mildew | Tomato | SlML | [193] |
Tomato | SlPMR4 | [194] | |
Wheat | TaMLO-A1 | [195] | |
Grapevine | VvMOL3 | [196] | |
Stem rot | Rapeseed | BnWRKY70 | [197] |
Black pod | Cacao | TcNPR3 | [198] |
Ergot | Rye | pyr4 and TrpE | [199] |
Bacteria | |||
Bacterial leaf blight | Rice | Pi21 or ERF922 | [192] |
OsSWEET14 | [200] | ||
Bacterial leaf spot | Tomato | SlJAZ2 | [201] |
Sldmr6-1 | [202] | ||
Banana Xanthomonas wilt | Musa balbisiana | LRR, WAK2, WAK5, Vicilin, RPM1, PR1, NPR1 | [203] |
Banana | MusaDMR6 | [204] | |
Bacterial speck | Tomato | SLDMR6-1 | [202] |
Virus | |||
Rice tungro spherical virus | Rice | eIF4G | [205,206] |
Rice black streaked dwarf virus | Rice | eIF4G | [207] |
Rice black-streaked dwarf virus | Maize | ZmGDIα | [208] |
Potato virus Y | Potato | eIF4E1 | [209] |
eIF4E | [210] | ||
Tomato brown rugose fruit virus | Tomato | TOM1 | [211] |
Tomato yellow leaf curl virus | Tomato | IR, CP of virus | [212] |
Soybean mosaic virus | Soybean | GmF3H1, GmF3H2 and GmFNSII-1 | [213] |
Cucumber vein yellowing virus, Zucchini yellow mosaic virus; Papaya ring spot mosaic virus- | Cucumber | eIF4G | [214] |
Banana streak virus | Banana | ORF1, ORF2,ORF3 of virus | [203] |
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Surya Krishna, S.; Harish Chandar, S.R.; Ravi, M.; Valarmathi, R.; Lakshmi, K.; Prathima, P.T.; Manimekalai, R.; Viswanathan, R.; Hemaprabha, G.; Appunu, C. Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges. Agronomy 2023, 13, 1000. https://doi.org/10.3390/agronomy13041000
Surya Krishna S, Harish Chandar SR, Ravi M, Valarmathi R, Lakshmi K, Prathima PT, Manimekalai R, Viswanathan R, Hemaprabha G, Appunu C. Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges. Agronomy. 2023; 13(4):1000. https://doi.org/10.3390/agronomy13041000
Chicago/Turabian StyleSurya Krishna, Sakthivel, S R Harish Chandar, Maruthachalam Ravi, Ramanathan Valarmathi, Kasirajan Lakshmi, Perumal Thirugnanasambandam Prathima, Ramaswamy Manimekalai, Rasappa Viswanathan, Govindkurup Hemaprabha, and Chinnaswamy Appunu. 2023. "Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges" Agronomy 13, no. 4: 1000. https://doi.org/10.3390/agronomy13041000