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28 pages, 2475 KiB  
Article
Optimal Scheduling of a Hydropower–Wind–Solar Multi-Objective System Based on an Improved Strength Pareto Algorithm
by Haodong Huang, Qin Shen, Wan Liu, Ying Peng, Shuli Zhu, Rungang Bao and Li Mo
Sustainability 2025, 17(15), 7140; https://doi.org/10.3390/su17157140 - 6 Aug 2025
Abstract
Under the current context of the large-scale integration of wind and solar power, the coupling of hydropower with wind and solar energy brings significant impacts on grid stability. To fully leverage the regulatory capacity of hydropower, this paper develops a multi-objective optimization scheduling [...] Read more.
Under the current context of the large-scale integration of wind and solar power, the coupling of hydropower with wind and solar energy brings significant impacts on grid stability. To fully leverage the regulatory capacity of hydropower, this paper develops a multi-objective optimization scheduling model for hydropower, wind, and solar that balances generation-side power generation benefit and grid-side peak-regulation requirements, with the latter quantified by the mean square error of the residual load. To efficiently solve this model, Latin hypercube initialization, hybrid distance framework, and adaptive mutation mechanism are introduced into the Strength Pareto Evolutionary Algorithm II (SPEAII), yielding an improved algorithm named LHS-Mutate Strength Pareto Evolutionary Algorithm II (LMSPEAII). Its efficiency is validated on benchmark test functions and a reservoir model. Typical extreme scenarios—months with strong wind and solar in the dry season and months with weak wind and solar in the flood season—are selected to derive scheduling strategies and to further verify the effectiveness of the proposed model and algorithm. Finally, K-medoids clustering is applied to the Pareto front solutions; from the perspective of representative solutions, this reveals the evolutionary trends of different objective trade-off schemes and overall distribution characteristics, providing deeper insight into the solution set’s distribution features. Full article
23 pages, 3036 KiB  
Article
Research on the Synergistic Mechanism Design of Electricity-CET-TGC Markets and Transaction Strategies for Multiple Entities
by Zhenjiang Shi, Mengmeng Zhang, Lei An, Yan Lu, Daoshun Zha, Lili Liu and Tiantian Feng
Sustainability 2025, 17(15), 7130; https://doi.org/10.3390/su17157130 - 6 Aug 2025
Abstract
In the context of the global response to climate change and the active promotion of energy transformation, a number of low-carbon policies coupled with the development of synergies to help power system transformation is an important initiative. However, the insufficient articulation of the [...] Read more.
In the context of the global response to climate change and the active promotion of energy transformation, a number of low-carbon policies coupled with the development of synergies to help power system transformation is an important initiative. However, the insufficient articulation of the green power market, tradable green certificate (TGC) market, and carbon emission trading (CET) mechanism, and the ambiguous policy boundaries affect the trading decisions made by its market participants. Therefore, this paper systematically analyses the composition of the main players in the electricity-CET-TGC markets and their relationship with each other, and designs the synergistic mechanism of the electricity-CET-TGC markets, based on which, it constructs the optimal profit model of the thermal power plant operators, renewable energy manufacturers, power grid enterprises, power users and load aggregators under the electricity-CET-TGC markets synergy, and analyses the behavioural decision-making of the main players in the electricity-CET-TGC markets as well as the electric power system to optimise the trading strategy of each player. The results of the study show that: (1) The synergistic mechanism of electricity-CET-TGC markets can increase the proportion of green power grid-connected in the new type of power system. (2) In the selection of different environmental rights and benefits products, the direct participation of green power in the market-oriented trading is the main way, followed by applying for conversion of green power into China certified emission reduction (CCER). (3) The development of independent energy storage technology can produce greater economic and environmental benefits. This study provides policy support to promote the synergistic development of the electricity-CET-TGC markets and assist the low-carbon transformation of the power industry. Full article
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21 pages, 1827 KiB  
Article
System Dynamics Modeling of Cement Industry Decarbonization Pathways: An Analysis of Carbon Reduction Strategies
by Vikram Mittal and Logan Dosan
Sustainability 2025, 17(15), 7128; https://doi.org/10.3390/su17157128 - 6 Aug 2025
Abstract
The cement industry is a significant contributor to global carbon dioxide emissions, primarily due to the energy demands of its production process and its reliance on clinker, a material formed through the high-temperature calcination of limestone. Strategies to reduce emissions include the adoption [...] Read more.
The cement industry is a significant contributor to global carbon dioxide emissions, primarily due to the energy demands of its production process and its reliance on clinker, a material formed through the high-temperature calcination of limestone. Strategies to reduce emissions include the adoption of low-carbon fuels, the use of carbon capture and storage (CCS) technologies, and the integration of supplementary cementitious materials (SCMs) to reduce the clinker content. The effectiveness of these measures depends on a complex set of interactions involving technological feasibility, market dynamics, and regulatory frameworks. This study presents a system dynamics model designed to assess how various decarbonization approaches influence long-term emission trends within the cement industry. The model accounts for supply chains, production technologies, market adoption rates, and changes in cement production costs. This study then analyzes a number of scenarios where there is large-scale sustained investment in each of three carbon mitigation strategies. The results show that CCS by itself allows the cement industry to achieve carbon neutrality, but the high capital investment results in a large cost increase for cement. A combined approach using alternative fuels and SCMs was found to achieve a large carbon reduction without a sustained increase in cement prices, highlighting the trade-offs between cost, effectiveness, and system-wide interactions. Full article
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32 pages, 2377 KiB  
Review
Antiplatelet Monotherapies for Long-Term Secondary Prevention Following Percutaneous Coronary Intervention
by Claudio Laudani, Daniele Giacoppo, Antonio Greco, Luis Ortega-Paz, Georges El Khoury, Davide Capodanno and Dominick J. Angiolillo
J. Clin. Med. 2025, 14(15), 5536; https://doi.org/10.3390/jcm14155536 - 6 Aug 2025
Abstract
In patients with coronary artery disease (CAD) undergoing percutaneous coronary intervention (PCI), antiplatelet therapy is the cornerstone of treatment for secondary prevention. Although dual antiplatelet therapy (DAPT) consisting of aspirin and a P2Y12 inhibitor is the current standard of care, being, respectively, [...] Read more.
In patients with coronary artery disease (CAD) undergoing percutaneous coronary intervention (PCI), antiplatelet therapy is the cornerstone of treatment for secondary prevention. Although dual antiplatelet therapy (DAPT) consisting of aspirin and a P2Y12 inhibitor is the current standard of care, being, respectively, recommended for 6 and 12 months in patients with chronic and acute coronary syndrome without a need for oral anticoagulation, the continuous improvement in PCI technology and pharmacology have significantly reduced the need for long-term DAPT. Mounting evidence supports the administration of P2Y12 inhibitor monotherapy, particularly ticagrelor, after a short period of DAPT following PCI as a strategy to reduce bleeding without a trade-off in ischemic events compared to standard DAPT. In addition, there is a growing literature supporting P2Y12 inhibitor monotherapy also for long-term secondary prevention of ischemic events. However, the data to this extent are not as robust as compared to the first-year post-PCI period, with aspirin monotherapy still remaining the mainstay of treatment for most patients. This review aims to summarize the rationale for long-term antiplatelet therapy, the pharmacology of current antiplatelet drugs tested for long-term administration as monotherapy, and current evidence on the available comparisons between different long-term antiplatelet monotherapies in patients with CAD. Full article
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27 pages, 1062 KiB  
Article
Dynamic Supply Chain Decision-Making of Live E-Commerce Considering Netflix Marketing Under Different Power Structures
by Yawen Liu, Mohammed Gadafi Tamimu and Junwu Chai
J. Theor. Appl. Electron. Commer. Res. 2025, 20(3), 202; https://doi.org/10.3390/jtaer20030202 - 6 Aug 2025
Abstract
The rapid growth of live e-commerce, a sector valued at over USD 100 billion worldwide, demonstrates its transformative impact on the retail industry, especially in markets like China, where platforms such as Taobao Live and TikTok Shop have markedly altered consumer interaction. This [...] Read more.
The rapid growth of live e-commerce, a sector valued at over USD 100 billion worldwide, demonstrates its transformative impact on the retail industry, especially in markets like China, where platforms such as Taobao Live and TikTok Shop have markedly altered consumer interaction. This transition is further expedited by Netflix-like entertainment marketing methods, which have demonstrated the capacity to enhance consumer retention by as much as 40%. As organizations adjust to this evolving landscape, it is essential to optimize supply chain strategies to align with these dynamic, consumer-centric environments. This paper examines the complexity of decision-making in live e-commerce supply chains, specifically regarding Netflix-inspired marketing strategies. The primary aim of this study is to design a game-theoretic framework that examines the interactions between producers and online celebrity retailers (OCRs) across different power dynamics. As live commerce integrates digital retail with immersive experiences, businesses must optimize pricing, quality, and marketing strategies in real-time. We present engagement-driven marketing as a strategic variable and incorporate consumer regret and switching costs into the demand function. To illustrate practical trade-offs in strategy, we incorporate a multi-criteria decision-making (MCDM) layer with AHP-TOPSIS, assessing profit, consumer surplus, engagement score, and channel efficiency. The experiment results indicate that Netflix-style marketing markedly increases demand and profit in retailer-led frameworks, whereas centralized tactics enhance overall channel performance. TOPSIS analysis prioritizes high-effort, high-engagement methods, whereas the Stackelberg experiment underscores the influence of power dynamics on profit distribution. This study presents an innovative integrative decision-making methodology for enhancing live-streaming commerce tactics in data-driven and consumer-focused markets. Full article
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28 pages, 2129 KiB  
Article
Research on Pricing Strategies of Knowledge Payment Products Considering the Impact of Embedded Advertising Under the User-Generated Content Model
by Xiubin Gu, Yi Qu and Minhe Wu
Systems 2025, 13(8), 665; https://doi.org/10.3390/systems13080665 - 6 Aug 2025
Abstract
In UGC-based knowledge trading platforms, the abundance of personalized content often leads to varying quality levels. By incorporating embedded advertising, platforms can incentivize knowledge producers to produce high-quality content; however, the uncertainty in managing embedded advertisements increases the complexity of pricing knowledge products. [...] Read more.
In UGC-based knowledge trading platforms, the abundance of personalized content often leads to varying quality levels. By incorporating embedded advertising, platforms can incentivize knowledge producers to produce high-quality content; however, the uncertainty in managing embedded advertisements increases the complexity of pricing knowledge products. This paper examines the impact of embedded advertising on the pricing of knowledge products, aims to maximize the profits of both knowledge producer and the platform. Based on Stackelberg game theory, two pricing decision models are developed under different advertising management modes: the platform-managed mode (where the platform determines the advertising intensity) and the advertiser-managed mode (where the advertiser determines the advertising intensity). The study analyzes the effects of UGC product quality, consumer sensitivity to advertising, and power structure on knowledge product pricing, and derives threshold conditions for optimal pricing. The results indicate that (1) When the quality of UGC knowledge product exceeds a certain threshold, platform-managed advertising becomes profitable. (2) Under the platform-managed mode, both the platform and knowledge producer can adopt price-increasing strategies to enhance profits. (3) Under the advertiser-managed mode, the platform can leverage differences in power structure to optimize revenue, while knowledge producer can actively enhance his pricing power to achieve mutual benefits with the platform. This study provides theoretical support and practical guidance for advertising cooperation mechanisms and pricing strategies for knowledge products in UGC-based knowledge trading platforms. Full article
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30 pages, 3996 KiB  
Article
Incentive-Compatible Mechanism Design for Medium- and Long-Term/Spot Market Coordination in High-Penetration Renewable Energy Systems
by Sicong Wang, Weiqing Wang, Sizhe Yan and Qiuying Li
Processes 2025, 13(8), 2478; https://doi.org/10.3390/pr13082478 - 6 Aug 2025
Abstract
In line with the goals of “peak carbon emissions and carbon neutrality”, this study aims to develop a market-coordinated operation mechanism to promote renewable energy adoption and consumption, addressing the challenges of integrating medium- and long-term trading with spot markets in power systems [...] Read more.
In line with the goals of “peak carbon emissions and carbon neutrality”, this study aims to develop a market-coordinated operation mechanism to promote renewable energy adoption and consumption, addressing the challenges of integrating medium- and long-term trading with spot markets in power systems with high renewable energy penetration. A three-stage joint operation framework is proposed. First, a medium- and long-term trading game model is established, considering multiple energy types to optimize the benefits of market participants. Second, machine learning algorithms are employed to predict renewable energy output, and a contract decomposition mechanism is developed to ensure a smooth transition from medium- and long-term contracts to real-time market operations. Finally, a day-ahead market-clearing strategy and an incentive-compatible settlement mechanism, incorporating the constraints from contract decomposition, are proposed to link the two markets effectively. Simulation results demonstrate that the proposed mechanism effectively enhances resource allocation and stabilizes market operations, leading to significant revenue improvements across various generation units and increased renewable energy utilization. Specifically, thermal power units achieve a 19.12% increase in revenue, while wind and photovoltaic units show more substantial gains of 38.76% and 47.52%, respectively. Concurrently, the mechanism drives a 10.61% increase in renewable energy absorption capacity and yields a 13.47% improvement in Tradable Green Certificate (TGC) utilization efficiency, confirming its overall effectiveness. This research shows that coordinated optimization between medium- and long-term/spot markets, combined with a well-designed settlement mechanism, significantly strengthens the market competitiveness of renewable energy, providing theoretical support for the market-based operation of the new power system. Full article
(This article belongs to the Section Energy Systems)
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35 pages, 3601 KiB  
Article
Carbon Emissions and Influencing Factors in the Areas Along the Belt and Road Initiative in Africa: A Spatial Spillover Perspective
by Suxin Yang and Miguel Ángel Benedicto Solsona
Sustainability 2025, 17(15), 7098; https://doi.org/10.3390/su17157098 - 5 Aug 2025
Abstract
The carbon dioxide spillover effects and influencing factors of the “Belt and Road Initiative” (BRI) in African countries must be assessed to evaluate the effectiveness, promote low-carbon transmissions in African countries, and provide recommendations for achieving the 2030 Sustainable Development Goals. This novel [...] Read more.
The carbon dioxide spillover effects and influencing factors of the “Belt and Road Initiative” (BRI) in African countries must be assessed to evaluate the effectiveness, promote low-carbon transmissions in African countries, and provide recommendations for achieving the 2030 Sustainable Development Goals. This novel study employs carbon dioxide emission intensity (CEI) and per capita carbon dioxide emissions (PCE) as dual indicators to evaluate the spatial spillover effects of 54 BRI African countries on their neighboring countries’ carbon emissions from 2007 to 2023. It identifies the key factors and mechanisms affecting these spillover effects using the spatial differences-in-differences (SDID) model. Results indicate that since the launch of the BRI, the CEI and PCE of BRI African countries have significantly increased, largely due to trade patterns and industrialization structures. Greater trade openness has further boosted local economic development, thereby increasing carbon dioxide’s spatial spillover. Government management and corruption control levels show some heterogeneity in the spillover effects, which may be attributed to long-standing issues of weak institutional enforcement in Africa. Overall, this study reveals the complex relationship between BRI African economic development and environmental outcomes, highlighting the importance of developing sustainable development strategies and establishing strong differentiated regulatory regimes to effectively address environmental challenges. Full article
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88 pages, 9998 KiB  
Review
Research and Developments of Heterogeneous Catalytic Technologies
by Milan Králik, Peter Koóš, Martin Markovič and Pavol Lopatka
Molecules 2025, 30(15), 3279; https://doi.org/10.3390/molecules30153279 - 5 Aug 2025
Abstract
This review outlines a comprehensive methodology for the research and development of heterogeneous catalytic technologies (R&D_HeCaTe). Emphasis is placed on the fundamental interactions between reactants, solvents, and heterogeneous catalysts—specifically the roles of catalytic centers and support materials (e.g., functional groups) in modulating activation [...] Read more.
This review outlines a comprehensive methodology for the research and development of heterogeneous catalytic technologies (R&D_HeCaTe). Emphasis is placed on the fundamental interactions between reactants, solvents, and heterogeneous catalysts—specifically the roles of catalytic centers and support materials (e.g., functional groups) in modulating activation energies and stabilizing catalytic functionality. Particular attention is given to catalyst deactivation mechanisms and potential regeneration strategies. The application of molecular modeling and chemical engineering analyses, including reaction kinetics, thermal effects, and mass and heat transport phenomena, is identified as essential for R&D_HeCaTe. Reactor configuration is discussed in relation to key physicochemical parameters such as molecular diffusivity, reaction exothermicity, operating temperature and pressure, and the phase and “aggressiveness” of the reaction system. Suitable reactor types—such as suspension reactors, fixed-bed reactors, and flow microreactors—are evaluated accordingly. Economic and environmental considerations are also addressed, with a focus on the complexity of reactions, selectivity versus conversion trade-offs, catalyst disposal, and separation challenges. To illustrate the breadth and applicability of the proposed framework, representative industrial processes are discussed, including ammonia synthesis, fluid catalytic cracking, methanol production, alkyl tert-butyl ethers, and aniline. Full article
(This article belongs to the Special Issue Heterogeneous Catalysts: From Synthesis to Application)
20 pages, 4989 KiB  
Article
Analysis of the Trade-Off/Synergy Effect and Driving Factors of Ecosystem Services in Hulunbuir City, China
by Shimin Wei, Jian Hou, Yan Zhang, Yang Tai, Xiaohui Huang and Xiaochen Guo
Agronomy 2025, 15(8), 1883; https://doi.org/10.3390/agronomy15081883 - 4 Aug 2025
Abstract
An in-depth understanding of the spatiotemporal heterogeneity of ecosystem service (ES) trade-offs and synergies, along with their driving factors, is crucial for formulating key ecological restoration strategies and effectively allocating ecological environmental resources in the Hulunbuir region. This study employed an integrated analytical [...] Read more.
An in-depth understanding of the spatiotemporal heterogeneity of ecosystem service (ES) trade-offs and synergies, along with their driving factors, is crucial for formulating key ecological restoration strategies and effectively allocating ecological environmental resources in the Hulunbuir region. This study employed an integrated analytical approach combining the InVEST model, ArcGIS geospatial processing, R software environment, and Optimal Parameter Geographical Detector (OPGD). The spatiotemporal patterns and driving factors of the interaction of four major ES functions in Hulunbuir area from 2000 to 2020 were studied. The research findings are as follows: (1) carbon storage (CS) and soil conservation (SC) services in the Hulunbuir region mainly show a distribution pattern of high values in the central and northeast areas, with low values in the west and southeast. Water yield (WY) exhibits a distribution pattern characterized by high values in the central–western transition zone and southeast and low values in the west. For forage supply (FS), the overall pattern is higher in the west and lower in the east. (2) The trade-off relationships between CS and WY, CS and SC, and SC and WY are primarily concentrated in the western part of Hulunbuir, while the synergistic relationships are mainly observed in the central and eastern regions. In contrast, the trade-off relationships between CS and FS, as well as FS and WY, are predominantly located in the central and eastern parts of Hulunbuir, with the intensity of these trade-offs steadily increasing. The trade-off relationship between SC and FS is almost widespread throughout HulunBuir. (3) Fractional vegetation cover, mean annual precipitation, and land use type were the primary drivers affecting ESs. Among these factors, fractional vegetation cover demonstrates the highest explanatory power, with a q-value between 0.6 and 0.9. The slope and population density exhibit relatively weak explanatory power, with q-values ranging from 0.001 to 0.2. (4) The interactions between factors have a greater impact on the inter-relationships of ESs in the Hulunbuir region than individual factors alone. The research findings have facilitated the optimization and sustainable development of regional ES, providing a foundation for ecological conservation and restoration in Hulunbuir. Full article
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24 pages, 1028 KiB  
Review
Biocontrol of Phage Resistance in Pseudomonas Infections: Insights into Directed Breaking of Spontaneous Evolutionary Selection in Phage Therapy
by Jumpei Fujiki, Daigo Yokoyama, Haruka Yamamoto, Nana Kimura, Manaho Shimizu, Hinatsu Kobayashi, Keisuke Nakamura and Hidetomo Iwano
Viruses 2025, 17(8), 1080; https://doi.org/10.3390/v17081080 - 4 Aug 2025
Abstract
Phage therapy, long overshadowed by antibiotics in Western medicine, has a well-established history in some Eastern European countries and is now being revitalized as a promising strategy against antimicrobial resistance (AMR). This resurgence of phage therapy is driven by the urgent need for [...] Read more.
Phage therapy, long overshadowed by antibiotics in Western medicine, has a well-established history in some Eastern European countries and is now being revitalized as a promising strategy against antimicrobial resistance (AMR). This resurgence of phage therapy is driven by the urgent need for innovative countermeasures to AMR, which will cause an estimated 10 million deaths annually by 2050. However, the emergence of phage-resistant variants presents challenges similar to AMR, thus necessitating a deeper understanding of phage resistance mechanisms and control strategies. The highest priority must be to prevent the emergence of phage resistance. Although phage cocktails targeting multiple receptors have demonstrated a certain level of phage resistance suppression, they cannot completely suppress resistance in clinical settings. This highlights the need for strategies beyond simple resistance suppression. Notably, recent studies examining fitness trade-offs associated with phage resistance have opened new avenues in phage therapy that offer the potential of restoring antibiotic susceptibility and attenuating pathogen virulence despite phage resistance. Thus, controlling phage resistance may rely on both its suppression and strategic redirection. This review summarizes key concepts in the control of phage resistance and explores evolutionary engineering as a means of optimizing phage therapy, with a particular focus on Pseudomonas infections. Harnessing evolutionary dynamics by intentionally breaking the spontaneous evolutionary trajectories of target bacterial pathogens could potentially reshape bacterial adaptation by acquisition of phage resistance, unlocking potential in the application of phage therapy. Full article
(This article belongs to the Section Bacterial Viruses)
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16 pages, 3766 KiB  
Article
Evaluation of Energy and CO2 Reduction Through Envelope Retrofitting: A Case Study of a Public Building in South Korea Conducted Using Utility Billing Data
by Hansol Lee and Gyeong-Seok Choi
Energies 2025, 18(15), 4129; https://doi.org/10.3390/en18154129 - 4 Aug 2025
Abstract
This study empirically evaluates the energy and carbon reduction effects of an envelope retrofit applied to an aging public building in South Korea. Unlike previous studies that primarily relied on simulation-based analyses, this work fills the empirical research gap by using actual utility [...] Read more.
This study empirically evaluates the energy and carbon reduction effects of an envelope retrofit applied to an aging public building in South Korea. Unlike previous studies that primarily relied on simulation-based analyses, this work fills the empirical research gap by using actual utility billing data collected over one pre-retrofit year (2019) and two post-retrofit years (2023–2024). The retrofit included improvements to exterior walls, roofs, and windows, aiming to enhance thermal insulation and airtightness. The analysis revealed that monthly electricity consumption was reduced by 14.7% in 2023 and 8.0% in 2024 compared to that in the baseline year, with corresponding decreases in electricity costs and carbon dioxide emissions. Seasonal variations were evident: energy savings were significant in the winter due to reduced heating demand, while cooling energy use slightly increased in the summer, likely due to diminished solar heat gains resulting from improved insulation. By addressing both heating and cooling impacts, this study offers practical insights into the trade-offs of envelope retrofitting. The findings contribute to the body of knowledge by demonstrating the real-world performance of retrofit technologies and providing data-driven evidence that can inform policies and strategies for improving energy efficiency in public buildings. Full article
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17 pages, 5658 KiB  
Communication
When DNA Tells the Tale: High-Resolution Melting as a Forensic Tool for Mediterranean Cetacean Identification
by Mariangela Norcia, Alessia Illiano, Barbara Mussi, Fabio Di Nocera, Emanuele Esposito, Anna Di Cosmo, Domenico Fulgione and Valeria Maselli
Int. J. Mol. Sci. 2025, 26(15), 7517; https://doi.org/10.3390/ijms26157517 - 4 Aug 2025
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Abstract
Effective species identification is crucial for the conservation and management of marine mammals, particularly in regions such as the Mediterranean Sea, where several cetacean populations are endangered or vulnerable. In this study, we developed and validated a High-Resolution Melting (HRM) analysis protocol for [...] Read more.
Effective species identification is crucial for the conservation and management of marine mammals, particularly in regions such as the Mediterranean Sea, where several cetacean populations are endangered or vulnerable. In this study, we developed and validated a High-Resolution Melting (HRM) analysis protocol for the rapid, cost-effective, and reliable identification of the four representative marine cetacean species that occur in the Mediterranean Sea: the bottlenose dolphin (Tursiops truncatus), the striped dolphin (Stenella coeruleoalba), the sperm whale (Physeter macrocephalus), and the fin whale (Balaenoptera physalus). Species-specific primers targeting mitochondrial DNA regions (cytochrome b and D-loop) were designed to generate distinct melting profiles. The protocol was tested on both tissue and fecal samples, demonstrating high sensitivity, reproducibility, and discrimination power. The results confirmed the robustness of the method, with melting curve profiles clearly distinguishing the target species and achieving a success rate > 95% in identifying unknown samples. The use of HRM offers several advantages over traditional sequencing methods, including reduced cost, speed, portability, and suitability for degraded samples, such as those from the stranded individuals. This approach provides a valuable tool for non-invasive genetic surveys and real-time species monitoring, contributing to more effective conservation strategies for cetaceans and enforcement of regulations against illegal trade. Full article
(This article belongs to the Special Issue Molecular Insights into Zoology)
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15 pages, 3854 KiB  
Article
PVC Inhibits Radish (Raphanus sativus L.) Seedling Growth by Interfering with Plant Hormone Signal Transduction and Phenylpropanoid Biosynthesis
by Lisi Jiang, Zirui Liu, Wenyuan Li, Yangwendi Yang, Zirui Yu, Jiajun Fan, Lixin Guo, Chang Guo and Wei Fu
Horticulturae 2025, 11(8), 896; https://doi.org/10.3390/horticulturae11080896 (registering DOI) - 3 Aug 2025
Viewed by 210
Abstract
Polyvinyl chloride (PVC) is commonly employed as mulch in agriculture to boost crop yields. However, its toxicity is often overlooked. Due to its chemical stability, resistance to degradation, and the inadequacy of the recycling system, PVC tends to persist in farm environments, where [...] Read more.
Polyvinyl chloride (PVC) is commonly employed as mulch in agriculture to boost crop yields. However, its toxicity is often overlooked. Due to its chemical stability, resistance to degradation, and the inadequacy of the recycling system, PVC tends to persist in farm environments, where it can decompose into microplastics (MPs) or nanoplastics (NPs). The radish (Raphanus sativus L.) was chosen as the model plant for this study to evaluate the underlying toxic mechanisms of PVC NPs on seedling growth through the integration of multi-omics approaches with oxidative stress evaluations. The results indicated that, compared with the control group, the shoot lengths in the 5 mg/L and 150 mg/L treatment groups decreased by 33.7% and 18.0%, respectively, and the root lengths decreased by 28.3% and 11.3%, respectively. However, there was no observable effect on seed germination rates. Except for the peroxidase (POD) activity in the 150 mg/L group, all antioxidant enzyme activities and malondialdehyde (MDA) levels were higher in the treated root tips than in the control group. Both transcriptome and metabolomic analysis profiles showed 2075 and 4635 differentially expressed genes (DEGs) in the high- and low-concentration groups, respectively, and 1961 metabolites under each treatment. PVC NPs predominantly influenced seedling growth by interfering with plant hormone signaling pathways and phenylpropanoid production. Notably, the reported toxicity was more evident at lower concentrations. This can be accounted for by the plant’s “growth-defense trade-off” strategy and the manner in which nanoparticles aggregate. By clarifying how PVC NPs coordinately regulate plant stress responses via hormone signaling and phenylpropanoid biosynthesis pathways, this research offers a scientific basis for assessing environmental concerns related to nanoplastics in agricultural systems. Full article
(This article belongs to the Special Issue Stress Physiology and Molecular Biology of Vegetable Crops)
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25 pages, 2100 KiB  
Article
Flexible Demand Side Management in Smart Cities: Integrating Diverse User Profiles and Multiple Objectives
by Nuno Souza e Silva and Paulo Ferrão
Energies 2025, 18(15), 4107; https://doi.org/10.3390/en18154107 - 2 Aug 2025
Viewed by 200
Abstract
Demand Side Management (DSM) plays a crucial role in modern energy systems, enabling more efficient use of energy resources and contributing to the sustainability of the power grid. This study examines DSM strategies within a multi-environment context encompassing residential, commercial, and industrial sectors, [...] Read more.
Demand Side Management (DSM) plays a crucial role in modern energy systems, enabling more efficient use of energy resources and contributing to the sustainability of the power grid. This study examines DSM strategies within a multi-environment context encompassing residential, commercial, and industrial sectors, with a focus on diverse appliance types that exhibit distinct operational characteristics and user preferences. Initially, a single-objective optimization approach using Genetic Algorithms (GAs) is employed to minimize the total energy cost under a real Time-of-Use (ToU) pricing scheme. This heuristic method allows for the effective scheduling of appliance operations while factoring in their unique characteristics such as power consumption, usage duration, and user-defined operational flexibility. This study extends the optimization problem to a multi-objective framework that incorporates the minimization of CO2 emissions under a real annual energy mix while also accounting for user discomfort. The Non-dominated Sorting Genetic Algorithm II (NSGA-II) is utilized for this purpose, providing a Pareto-optimal set of solutions that balances these competing objectives. The inclusion of multiple objectives ensures a comprehensive assessment of DSM strategies, aiming to reduce environmental impact and enhance user satisfaction. Additionally, this study monitors the Peak-to-Average Ratio (PAR) to evaluate the impact of DSM strategies on load balancing and grid stability. It also analyzes the impact of considering different periods of the year with the associated ToU hourly schedule and CO2 emissions hourly profile. A key innovation of this research is the integration of detailed, category-specific metrics that enable the disaggregation of costs, emissions, and user discomfort across residential, commercial, and industrial appliances. This granularity enables stakeholders to implement tailored strategies that align with specific operational goals and regulatory compliance. Also, the emphasis on a user discomfort indicator allows us to explore the flexibility available in such DSM mechanisms. The results demonstrate the effectiveness of the proposed multi-objective optimization approach in achieving significant cost savings that may reach 20% for industrial applications, while the order of magnitude of the trade-offs involved in terms of emissions reduction, improvement in discomfort, and PAR reduction is quantified for different frameworks. The outcomes not only underscore the efficacy of applying advanced optimization frameworks to real-world problems but also point to pathways for future research in smart energy management. This comprehensive analysis highlights the potential of advanced DSM techniques to enhance the sustainability and resilience of energy systems while also offering valuable policy implications. Full article
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