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Proper joint function has a significant impact on people’s quality of life. Joints are the point of connection between two or more bones and consist of at least three elements: joint surfaces, the joint capsule, and the joint cavity. Joint diseases are a serious social problem. Risk factors for the development of these diseases include overweight and obesity, gender, and intestinal microbiome disorders. Another factor that is considered to influence joint diseases is trace elements. Under normal conditions, elements such as iron (Fe), copper (Cu), cobalt (Co), iodine (I), manganese (Mn), zinc (Zn), silver (Ag), cadmium (Cd), mercury (Hg), lead (Pb), nickel (Ni) selenium (Se), boron (B), and silicon (Si) are part of enzymes involved in reactions that determine the proper functioning of cells, regulate redox metabolism, and determine the maturation of cells that build joint components. However, when the normal concentration of the above-mentioned elements is disturbed and toxic elements are present, dangerous joint diseases can develop. In this article, we focus on the role of trace elements in joint function. We describe the molecular mechanisms that explain their interaction with chondrocytes, osteocytes, osteoblasts, osteoclasts, and synoviocytes, as well as their proliferation, apoptosis, and extracellular matrix synthesis. We also focus on the role of these trace elements in the pathogenesis of joint diseases: rheumatoid arthritis (RA), osteoarthritis (OA), psoriatic arthritis (PsA), ankylosing spondylitis (AS), and systemic lupus erythematosus (SLE). We describe the roles of increased or decreased concentrations of individual elements in the pathogenesis and development of joint diseases and their impact on inflammation and disease progression, referring to molecular mechanisms. We also discuss their potential application in the treatment of joint diseases. Full article

Due to the feeding system (high-concentrate diet) during the early lactation stage, ruminal pH in dairy cows follows a diurnal pattern and can remain below the critical level of 5.5 for extended periods of the day. This study aimed to evaluate the effect of low ruminal pH on blood concentrations of certain macrominerals, trace minerals, and fat-soluble vitamins and on the oxidative status of dairy cows during the first half of lactation. Fifty-three randomly selected lactating Holstein cows were used; blood and ruminal fluid samples were collected from all cows on days 30, 90 and 150 of lactation. Blood samples were obtained via coccygeal venipuncture, while the ruminal fluid was obtained by rumenocentesis and the pH was measured immediately after collection. Using a threshold pH of 5.5, samples were classified as normal (pH > 5.5) or low pH (pH ≤ 5.5). Serum concentrations of Ca, Mg, K, Cr, Mn, Zn, Se, and vitamins A, D3, E, and K were not significantly affected by ruminal pH, either by days in milk or by their interaction (p > 0.05). Plasma malondialdehyde and reduced glutathione followed the same trend (p > 0.05). Copper concentration was significantly higher (p < 0.05), and Fe concentration tended to be higher in cows with low pH compared to those with normal pH (p = 0.052). On day 150 of lactation, Cu, Fe, and Co concentrations were significantly higher in low-pH cows compared to normal-pH cows (p < 0.05). Low ruminal pH is associated with significant changes in serum concentrations of copper, iron, and cobalt but has no significant effect on the oxidative status of the animals or on the serum concentrations of the macro elements and fat-soluble vitamins studied. Full article

The Junggar Basin contains a large amount of coal resources and is an important coal production base in China. The coal seam in Zhundong coalfield has a large single-layer thickness and high content of inertinite, but large particle Fe-sulphide minerals are associated with coal seams in some mining areas. A series of economic and environmental problems caused by the combustion of large-grained Fe-sulphide minerals in coal have seriously affected the economic, clean and efficient utilization of coal. In this paper, the ultra-thick coal seam of the Xishanyao formation in the Yihua open-pit mine of the Zhundong coalfield is taken as the research object. Through the analysis of coal quality, X-ray fluorescence spectrometer test of major elements in coal, inductively coupled plasma mass spectrometry test of trace elements, SEM-Raman identification of Fe-sulphide minerals in coal and LA-MC-ICP-MS test of sulfur isotope of marcasite, the coal quality characteristics, main and trace element characteristics, macro and micro occurrence characteristics of Fe-sulphide minerals and sulfur isotope characteristics of marcasite in the ultra-thick coal seam of the Xishanyao formation are tested. On this basis, the occurrence state and genesis of large particle Fe-sulphide minerals in the ultra-thick coal seam of the Xishanyao formation are clarified. The main results and understandings are as follows: (1) the occurrence state of Fe-sulphide minerals in extremely thick coal seams is clarified. The Fe-sulphide minerals in the extremely thick coal seam are mainly marcasite, and concentrated in the YH-2, YH-3, YH-8, YH-9, YH-14, YH-15 and YH-16 horizons. Macroscopically, Fe-sulphide minerals mainly occur in three forms: thin film Fe-sulphide minerals, nodular Fe-sulphide minerals, and disseminated Fe-sulphide minerals. Microscopically, they mainly occur in four forms: flake, block, spearhead, and crack filling. (2) The difference in sulfur isotope of marcasite was discussed, and the formation period of marcasite was preliminarily divided. The overall variation range of the δ³⁴S value of marcasite is wide, and the extreme values are quite different. The polyflake marcasite was formed in the early stage of diagenesis and the δ³⁴S value was negative, while the fissure filling marcasite was formed in the late stage of diagenesis and the δ³⁴S value was positive. (3) The coal quality characteristics of the thick coal seam were analyzed. The organic components in the thick coal seam are mainly inertinite, and the inorganic components are mainly clay minerals and marcasite. (4) The difference between the element content in the thick coal seam of the Zhundong coalfield and the average element content of Chinese coal was compared. The major element oxides in the thick coal seam are mainly CaO and MgO, followed by SiO₂, Al₂O₃, Fe₂O₃ and Na₂O. Li, Ga, Ba, U and Th are enriched in trace elements. (5) The coal-accumulating environment characteristics of the extremely thick coal seam are revealed. The whole thick coal seam is formed in an acidic oxidation environment, and the horizon with Fe-sulphide minerals is in an acidic reduction environment. The acidic reduction environment is conducive to the formation of marcasite and is not conducive to the formation of pyrite. (6) There are many matrix vitrinite, inertinite content, clay content, and terrigenous debris in the extremely thick coal seam. The good supply of peat swamp, suitable reduction environment and pH value, as well as groundwater leaching and infiltration, together cause the occurrence of large-grained Fe-sulphide minerals in the extremely thick coal seam of the Xishanyao formation in the Zhundong coalfield. Full article

Selenium (Se) is an important trace element in our body; however, food composition data remain limited due to analytical challenges and interferences. Seafood, abundant in Thailand, is recognized as a rich source of Se. This study aimed to expand knowledge on Se content in seafood prepared using traditional Thai cooking methods. Twenty seafood species were selected and prepared by boiling, frying, and grilling. Inductively Coupled Plasma–Triple Quadrupole–Mass Spectrometry (ICP-MS/MS) was used to analyze total Se contents in selected seafood species. Results revealed significant variation in Se content across species and cooking methods. The Indo-Pacific horseshoe crab showed the highest Se concentration, with fried samples reaching 193.9 μg/100 g. Se concentrations were in the range of 8.6–155.5 μg/100 g (fresh), 14.3–106.6 μg/100 g (boiled), 17.3–193.9 μg/100 g (fried), and 7.3–160.1 μg/100 g (grilled). Results found significant effects of species and cooking method on Se content (p < 0.05). Fried seafood exhibited the highest estimated marginal mean Se concentration (a 78.8 μg/100 g edible portion), significantly higher than other methods. True retention (%TR) of Se ranged from 40.4% to 100%, depending on species and method. Bigfin reef squid, wedge shell, and silver pomfret showed the highest %TR (100%), while splendid squid exhibited the lowest (52.5%). Significant interaction effects on %TR were also observed (p < 0.05). Fried seafood had the highest mean %TR (88.8%), followed by grilled (82.1%) and boiled (79.7%). These findings highlight the effects of both species and cooking method on Se retention, emphasizing the nutritional value of selected seafood in preserving bioavailable Se after cooking. Full article

High-purity quartz (HPQ), an indispensable industrial mineral, serves as a critical raw material for advanced technology sectors. Derived from natural quartz precursors through processing, HPQ preparation efficiency fundamentally depends on raw material selection. Two pegmatite samples (muscovite pegmatite and two-mica pegmatite) sampled from the eastern sector of the North Qinling Orogenic Belt were investigated through a suite of analytical techniques, as well as processing and purification, to evaluate their potential as raw materials for high-purity quartz. Muscovite pegmatite is predominantly composed of quartz, plagioclase, K-feldspar, muscovite, and garnet, with accessory phases including limonite and kaolinite. However, in addition to quartz, plagioclase, K-feldspar, muscovite, garnet, and limonite, two-mica pegmatite contains minerals such as biotite and calcite. The fluid inclusions in both muscovite and two-mica pegmatite quartz are small, but the former has fewer fluid inclusions. Compared with muscovite pegmatite, surface discontinuity (i.e., cracks, pits, cavities) development is more pronounced in two-mica pegmatite purified quartz, which may be related to its high content of fluid inclusions. Following purification, the total concentration of trace elements decreased significantly. However, the concentrations of Al and Ti appeared to remain the same. Titanium enrichment in purified two-mica pegmatite quartz likely derives from biotite, while Na and Ca concentrations may be related to fluid inclusions or microscopic mineral inclusions. The trace element content (27.69 ppm) in muscovite pegmatite is lower than that (45.28 ppm) of two-mica pegmatite, we thus suggest that muscovite pegmatite quartz is more likely to have the potential to produce high-purity quartz. Full article

This study investigates growth responses, heavy metal (Cd, As) uptake, translocation, and mineral nutrient regulation in leafy vegetables with varying heavy metal tolerance, addressing the threat posed by combined Cd and As pollution. Three high-tolerance, four moderate-tolerance, and one sensitive leafy vegetable were grown in Cd+As-contaminated hydroponics. Post-harvest yields and concentrations of Cd, As, and trace elements were assessed. Results showed that (1) compared with single heavy metal treatments, the combination of Cd and As significantly increased the translocation factor of Cd in black bean sprouts and white radish sprouts by up to 83.83% and 503.2%; (2) changes in mineral nutrient concentrations in leafy vegetables were similar between single and combined heavy metal stresses, but the regulatory patterns varied among different leafy vegetable species; (3) under Cd/As exposure, high-tolerance leafy vegetables (e.g., pak choi) had strong heavy metal accumulation abilities, and heavy metal stress positively regulated mineral elements in their roots; In contrast, sensitive leafy vegetables (e.g., pea sprouts) often exhibited suppressed mineral element content in their roots, which was a result of their strategy to reduce heavy metal uptake. These results offer key insights into resistance mechanisms against combined heavy metal pollution in leafy vegetables, supporting phytoremediation efforts and safe production. Full article

Tailoring culture media and supplementation strategies to the specific requirements of a target product is essential for enhancing microbial production efficiency. This work addresses an unexplored aspect of K. phaffii cultivation: optimizing culture media for metabolite production from xylose, diverging from the conventional focus on recombinant protein expression and the use of glycerol or methanol as primary substrates. Ethylene glycol biosynthesis in an engineered K. phaffii strain was improved by evaluating media and nutrient supplementation. Among the seven evaluated formulations, FM22 and d’Anjou were the most effective, with inositol and thiamine dichloride playing key roles in enhancing production. Salt concentrations in both media were optimized using Central Composite Design (CCD), reducing complexity while increasing yields. Ethylene glycol production increased by 54% in FM22 and 21% in d’Anjou, accompanied by a threefold and 26% reduction in the total salt content, respectively. The vitamin solution was streamlined from seven to two components, each at half the standard concentration. Trace element solutions were reduced to 25% of the original volume without compromising productivity. These findings underscore the dual benefit of culture medium optimization: improved ethylene glycol yields and simplified formulations, establishing a foundation for the development of more efficient and cost-effective bioprocesses using K. phaffii. Full article

Unionid mussel populations in a section of the Clinch River in Virginia, USA, has declined substantially, but the causes of the decline remain unknown. To investigate this zone of decline (ZOD), we deployed juvenile freshwater mussels (Villosa iris in 2012 and Lampsilis fasciola in 2013) in both cages and silos at sites within the Clinch River System. We analyzed mussel tissues for trace element and organic contaminant concentrations, shells for trace elements, and environmental media (total water, dissolved water, particulate sediment, and bedload sediment) for both inorganic and organic contaminants. We found a few differences between mussels deployed in cages and those deployed in silos: survival was slightly lower in cages due to periodic sedimentation. Our results identified the ZOD based on the accumulation of trace elements (notably As, Cu, Fe, Mn, Ni, and Sr), polycyclic aromatic hydrocarbons (PAHs), and δ¹⁵N enrichment, with especially high concentrations found in the human-impacted tributaries, Dumps Creek and Guest River. Some correlations were found between environmental media and both mussel tissues and shells. In particular, PAHs and Mn had several significant relationships between bioaccumulated concentrations and environmental concentrations. Finally, Co, Cu, Fe, and V in soft tissues negatively correlated with mussel growth, whereas bioaccumulated PAH concentrations correlated negatively with resident mussel densities. Full article

The Qiubudong silver deposit on the western margin of the Fuping ore cluster in the central North China Craton is a representative breccia-type deposit characterized by relatively high-grade ores, thick mineralized zones, and extensive alteration, indicating considerable potential for economic resource development and further exploration. Previous studies on this deposit have not addressed its genetic mineralogical characteristics. This study focuses on pyrite and quartz to investigate their typomorphic features, such as crystal morphology, trace element composition, thermoelectric properties, and luminescence characteristics, and their implications for ore-forming processes. Pyrite crystals are predominantly cubic in early stages, while pentagonal dodecahedral and cubic–dodecahedral combinations peak during the main mineralization stage. The pyrite is sulfur-deficient and iron-rich, enriched in Au, and relatively high in Ag, Cu, Pb, and Bi contents during the main ore-forming stage. Rare earth element (REE) concentrations are low, with weak LREE-HREE fractionation and a strong negative Eu anomaly. The thermoelectric coefficient of pyrite ranges from −328.9 to +335.6 μV/°C, with a mean of +197.63 μV/°C; P-type conduction dominates, with an occurrence rate of 58%–100% and an average of 88.78%. A weak–low temperature and a strong–high temperature peak characterize quartz thermoluminescence during the main mineralization stage. Fluid inclusions in quartz include liquid-rich, vapor-rich, and two-phase types, with salinities ranging from 10.11% to 12.62% NaCl equiv. (average 11.16%) and densities from 0.91 to 0.95 g/cm³ (average 0.90 g/cm³). The ore-forming fluids are interpreted as F-rich, low-salinity, low-density hydrothermal fluids of volcanic origin at medium–low temperatures. The abundance of pentagonal dodecahedral pyrite, low Co/Ni ratios, high Cu contents, and complex quartz thermoluminescence signatures are key mineralogical indicators for deep prospecting. Combined with thermoelectric data and morphological analysis, the depth interval around 800 m between drill holes ZK3204 and ZK3201 has high mineralization potential. This study fills a research gap on the genetic mineralogy of the Qiubudong deposit and provides a scientific basis for deep exploration. Full article

Vegetables and fruits grown in urban areas pose a potential threat to human health due to contamination with heavy metals (HMs). This study aimed to identify and quantify the concentrations of heavy metals (Fe, Mn, Zn, Cu, Pb, Cd) in tomatoes, leafy vegetables, and fruits collected from 16 allotment gardens (AGs) located in Warsaw. A total of 112 samples were analyzed (72 vegetable and 40 fruit samples). Vegetables from AGs accumulated significantly higher levels of HMs than fruits. Leafy vegetables, particularly those cultivated near high-traffic roads, exhibited markedly elevated levels of Pb, Cd, and Zn compared to those grown in peripheral areas. Lead concentrations exceeded permissible limits by six to twelve times, cadmium by one to thirteen times, and zinc by 0.7 to 2.4 times. Due to high levels of Pb and Cd, tomatoes should not be cultivated in urban environments. Regardless of location, only trace amounts of HMs were detected in fruits. The greatest health risk is associated with the consumption of leafy vegetables. Lettuce should be considered an indicator plant for assessing environmental contamination. The obtained Hazard Index (HI) values indicate that only the tested fruits are safe for consumption. Meanwhile, the values of the Hazard Quotient (HQ) indicate no health risk associated with the consumption of lettuce, cherries, and red currants. Among the analyzed elements, Pb showed a higher potential health risk than other metals. This study emphasizes the need for continuous monitoring of HM levels in urban soils and the establishment of baseline values for public health purposes. Remediation of contaminated soils and the implementation of safer agricultural practices are recommended to reduce the exposure of urban populations to the risks associated with the consumption of contaminated produce. In addition, the safety of fruits and vegetables grown in urban areas is influenced by the location of the AGs and the level of industrialization of the agglomeration. Therefore, the safety assessment of plant products derived from AGs should be monitored on a continuous basis, especially in vegetables. Full article

This study presents a methodology for developing and validating digital models of tooth-inlay systems, aiming to trace the complete workflow from clinical procedures to simulation by involving dental professionals—dentists for manual cavity preparation and dental technicians for restoration modelling—while integrating micro-computed tomography (micro-CT) imaging with finite element analysis (FEA). The proposed workflow includes (1) the acquisition of high-resolution 3D micro-CT scans of a non-restored tooth, (2) image segmentation and reconstruction to create anatomically accurate digital twins and mesh generation, (3) the selection of proper resin and the 3D printing of four typodonts, (4) the manual preparation of cavities on the typodonts, (5) the acquisition of high-resolution 3D micro-CT scans of the typodonts, (6) mesh generation, digital inlay and onlay modelling and material property assignment, and (7) nonlinear FEA simulations under representative masticatory loading. The approach enables the visualisation of stress and deformation patterns, with preliminary results indicating stress concentrations at the tooth-restoration interface integrating different cavity alternatives and restorations on the same tooth. Quantitative outputs include von Mises stress, strain energy density, and displacement distribution. This study demonstrates the feasibility of using image-based, tooth-specific digital twins for biomechanical modelling in dentistry. The developed framework lays the groundwork for future investigations into the optimisation of restoration design and material selection in clinical applications. Full article

With the increasing market demand for spinels of various colors, purple spinel—long regarded as a symbol of nobility—has attracted growing attention. In this study, pinkish-purple to purple spinels from the Mogok region of Myanmar were systematically examined using conventional gemological, spectroscopic, and chemical analytical techniques. Raman analysis reveals that these spinels commonly contain octahedral inclusions composed of calcite, dolomite, magnesite, and graphite. Chemically, the samples are primarily magnesia-alumina spinels. Color variation is influenced by trace elements: increasing Cr and V contents enhance the red hue, while higher Fe concentrations intensify the purple tone. UV–Vis spectra show that Cr³⁺ and V³⁺ jointly contribute to absorptions at 388 nm and 548 nm, with Fe²⁺ and Fe³⁺ responsible for the bands at 371 nm and 457 nm, respectively, together controlling the pink-to-purple color variation. Most samples display four Cr³⁺-related peaks near 700 nm; however, these are absent in deeply purple spinels. In contrast, light pink spinels show weaker absorption at 371 nm and 457 nm, attributed to Fe²⁺ and Fe³⁺. Fluorescence spectra confirm characteristic Cr³⁺ emission bands at 673 nm, 684 nm, 696 nm, 706 nm, and 716 nm, indicating a strong crystal field environment. Raman spectra have peaks mainly around 312 cm⁻¹, 406 cm⁻¹, 665 cm⁻¹, and 768 cm⁻¹. The peaks of the infrared spectrum mainly appear around 840 cm⁻¹, 729 cm⁻¹, 587 cm⁻¹, 545 cm⁻¹, and 473 cm⁻¹. Full article

Feathers are commonly used to monitor trace elements in birds, including heavy metals. Typically, a single feather is analyzed to avoid harming living birds, assuming it reflects the organism’s overall contamination. To verify this assumption, we analyzed mercury concentrations in 12 flight and contour feathers from 25 barn swallows Hirundo rustica (16 adults and nine juveniles) that had died accidentally in a colony of the Po Plain (northern Italy). The median concentration in all feathers examined was 1.03 µg g⁻¹ in adults (range 0.76 µg g⁻¹–1.30 µg g⁻¹) and 0.39 µg g⁻¹ in juveniles (range 0.28 µg g⁻¹–0.71 µg g⁻¹), which is consistent with the results of similar research carried out on other world regions. No significant differences were observed between sexes, whereas marked differences were observed between adults and juveniles. In adults, mercury concentration was similar across remiges, rectrices, and contour feathers while in juveniles it was higher in contour feathers than in flight feathers. Mercury accumulation was highest in primary remiges and contour feathers, accounting for 67.6% of total mercury in adults and 77.5% in juveniles. However, primary remiges cannot be collected from live adults due to their importance in flight. In juveniles, contour feathers carry about 50% of total mercury, suggesting ventral and dorsal plumage may be useful for assessing mercury burden. Our findings are consistent with the hypothesis that mercury accumulation in feathers aids detoxification, with early-molted feathers (primary remiges and contour feathers) containing higher mercury levels than those replaced later (rectrices and secondary remiges). Full article

A novel single-walled carbon nanotube (SWNT), silver (Ag) nanoparticle, and zinc benzene carboxylate (ZnBDC) metal–organic framework (MOF) composite was synthesised and systematically characterised to develop an efficient platform for mercury ion (Hg²⁺) detection. X-ray diffraction confirmed the successful incorporation of Ag nanoparticles and SWNTs without disrupting the crystalline structure of ZnBDC. Meanwhile, field-emission scanning electron microscopy and energy-dispersive spectroscopy mapping revealed a uniform elemental distribution. Thermogravimetric analysis indicated enhanced thermal stability. Electrochemical measurements (cyclic voltammetry and electrochemical impedance spectroscopy) demonstrated improved charge transfer properties. Electrochemical sensing investigations using differential pulse voltammetry revealed that the SWNTs/Ag@ZnBDC-modified glassy carbon electrode exhibited high selectivity toward Hg²⁺ ions over other metal ions (Cd²⁺, Co²⁺, Cr³⁺, Fe³⁺, and Zn²⁺), with optimal performance at pH 4. The sensor displayed a linear response in the concentration range of 0.1–1.0 nM (R² = 0.9908), with a calculated limit of detection of 0.102 nM, slightly close to the lowest tested point, confirming its high sensitivity for ultra-trace Hg²⁺ detection. The outstanding sensitivity, selectivity, and reproducibility underscore the potential of SWNTs/Ag@ZnBDC as a promising electrochemical platform for detecting trace levels of Hg2+ in environmental monitoring. Full article

This study investigated the accumulation characteristics and associated health risks of 11 trace elements (Al, Rb, Cr, Ni, Mo, Sr, Pb, Ba, Ag, As, and Ga) in four crucian carp varieties: gene-edited intermuscular bone-free crucian carp (Carassius auratus, WUCI) and its sibling wild-type (Carassius auratus, WT), Fangzheng silver crucian carp (Carassius gibelio var Fangzheng, FZYJ), and Songpu silver crucian carp (Carassius gibelio var Songpu, SPYJ). Results showed that Al and Rb were the most abundant elements across all groups. WUCI exhibited distinct accumulation patterns, including significantly higher hepatic Mo concentrations (0.265 ± 0.032 mg/kg) and muscle/liver Rb levels (muscle: 8.74 ± 1.21 mg/kg; liver: 12.56 ± 2.05 mg/kg) compared to other varieties (p < 0.05), which supports the hypothesis of genotype-specific differences in heavy metal accumulation. Correlation analysis revealed that WUCI exhibited similar elemental interactions with WT and SPYJ (e.g., Al-Ni positive correlation, |rs| ≥ 0.8), while SPYJ displayed distinct patterns with fifteen negative correlations compared to three to five in others varieties, suggesting a potential alteration in elemental homeostasis. Pollution index (P_i) assessments indicated mild contamination for Pb in SPYJ liver (P_i = 0.265) and Cr/As in WUCI muscle (P_i = 0.247/0.218). Despite these values, all hazard indices remained below the established safety thresholds (THQ < 0.1, HI < 0.25, TCR < 10⁻⁶), reinforcing the overall safety of the tested fish. Notably, muscle As levels (0.86 ± 0.15 mg/kg) exceeded hepatic concentrations (0.52 ± 0.09 mg/kg), potentially due to differential detoxification mechanisms. These findings demonstrate the food safety of all tested varieties, while highlighting genotype-specific metabolic adaptations, providing critical data for evaluating gene edited aquatic products. Full article