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Search Results (1,513)

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17 pages, 1783 KiB  
Article
Nature-Based Solutions in Sustainable Cities: Trace Metal Accumulation in Urban Forests of Vienna (Austria) and Krakow (Poland)
by Mateusz Jakubiak, Ewa Panek, Krzysztof Urbański, Sónia Silva Victória, Stanisław Lach, Kamil Maciuk and Marek Kopacz
Sustainability 2025, 17(15), 7042; https://doi.org/10.3390/su17157042 (registering DOI) - 3 Aug 2025
Abstract
Forests are considered one of the most valuable natural areas in metropolitan region landscapes. Considering the sensitivity and ecosystem services provided by trees, the definition of urban forest ecosystems is nowadays based on a comprehensive understanding of the entire urban ecosystem. The effective [...] Read more.
Forests are considered one of the most valuable natural areas in metropolitan region landscapes. Considering the sensitivity and ecosystem services provided by trees, the definition of urban forest ecosystems is nowadays based on a comprehensive understanding of the entire urban ecosystem. The effective capturing of particulate matter is one of the ecosystem services provided by urban forests. These ecosystems function as efficient biological filters. Plants accumulate pollutants passively via their leaves. Therefore, another ecosystem service provided by city forests could be the use of tree organs as bioindicators of pollution. This paper aims to estimate differences in trace metal pollution between the wooded urban areas of Vienna and Krakow using leaves of evergreen and deciduous trees as biomonitors. An additional objective of the research was to assess the ability of the applied tree species to act as biomonitors. Plant samples of five species—Norway spruce, Scots pine, European larch, common white birch, and common beech—were collected within both areas, in seven locations: four in the “Wienerwald” Vienna forest (Austria) and three in the “Las Wolski” forest in Krakow (Poland). Concentrations of Cr, Cu, Cd, Pb, and Zn in plant material were determined. Biomonitoring studies with deciduous and coniferous tree leaves showed statistically higher heavy metal contamination in the “Las Wolski” forest compared to the “Wienerwald” forest. Based on the conducted analyses and the literature study, it can be concluded that among the analyzed tree species, only two: European beech and common white birch can be considered potential indicators in environmental studies. These species appear to be suitable bioindicators, as both are widespread in urban woodlands of Central Europe and have shown the highest accumulation levels of trace metals. Full article
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16 pages, 744 KiB  
Article
Investigation of Effects of Low Ruminal pH Values on Serum Concentrations of Macrominerals, Trace Elements, and Vitamins and Oxidative Status of Dairy Cows
by Panagiotis D. Katsoulos, Bengü Bilgiç, Duygu Tarhan, Fatma Ateş, Suat Ekin, Süleyman Kozat, Banu Dokuzeylül, Mehmet Erman Or, Emmanouil Kalaitzakis, Georgios E. Valergakis and Nikolaos Panousis
Ruminants 2025, 5(3), 35; https://doi.org/10.3390/ruminants5030035 (registering DOI) - 2 Aug 2025
Viewed by 44
Abstract
Due to the feeding system (high-concentrate diet) during the early lactation stage, ruminal pH in dairy cows follows a diurnal pattern and can remain below the critical level of 5.5 for extended periods of the day. This study aimed to evaluate the effect [...] Read more.
Due to the feeding system (high-concentrate diet) during the early lactation stage, ruminal pH in dairy cows follows a diurnal pattern and can remain below the critical level of 5.5 for extended periods of the day. This study aimed to evaluate the effect of low ruminal pH on blood concentrations of certain macrominerals, trace minerals, and fat-soluble vitamins and on the oxidative status of dairy cows during the first half of lactation. Fifty-three randomly selected lactating Holstein cows were used; blood and ruminal fluid samples were collected from all cows on days 30, 90 and 150 of lactation. Blood samples were obtained via coccygeal venipuncture, while the ruminal fluid was obtained by rumenocentesis and the pH was measured immediately after collection. Using a threshold pH of 5.5, samples were classified as normal (pH > 5.5) or low pH (pH ≤ 5.5). Serum concentrations of Ca, Mg, K, Cr, Mn, Zn, Se, and vitamins A, D3, E, and K were not significantly affected by ruminal pH, either by days in milk or by their interaction (p > 0.05). Plasma malondialdehyde and reduced glutathione followed the same trend (p > 0.05). Copper concentration was significantly higher (p < 0.05), and Fe concentration tended to be higher in cows with low pH compared to those with normal pH (p = 0.052). On day 150 of lactation, Cu, Fe, and Co concentrations were significantly higher in low-pH cows compared to normal-pH cows (p < 0.05). Low ruminal pH is associated with significant changes in serum concentrations of copper, iron, and cobalt but has no significant effect on the oxidative status of the animals or on the serum concentrations of the macro elements and fat-soluble vitamins studied. Full article
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15 pages, 675 KiB  
Article
A Trusted Multi-Cloud Brokerage System for Validating Cloud Services Using Ranking Heuristics
by Rajganesh Nagarajan, Vinothiyalakshmi Palanichamy, Ramkumar Thirunavukarasu and J. Arun Pandian
Future Internet 2025, 17(8), 348; https://doi.org/10.3390/fi17080348 (registering DOI) - 31 Jul 2025
Viewed by 144
Abstract
Cloud computing offers a broad spectrum of services to users, particularly in multi-cloud environments where service-centric features are introduced to support users from multiple endpoints. To improve service availability and optimize the utilization of required services, cloud brokerage has been integrated into multi-cloud [...] Read more.
Cloud computing offers a broad spectrum of services to users, particularly in multi-cloud environments where service-centric features are introduced to support users from multiple endpoints. To improve service availability and optimize the utilization of required services, cloud brokerage has been integrated into multi-cloud systems. The primary objective of a cloud broker is to ensure the quality and outcomes of services offered to customers. However, traditional cloud brokers face limitations in measuring service trust, ensuring validity, and anticipating future enhancements of services across different cloud platforms. To address these challenges, the proposed intelligent cloud broker integrates an intelligence mechanism that enhances decision-making within a multi-cloud environment. This broker performs a comprehensive validation and verification of service trustworthiness by analyzing various trust factors, including service response time, sustainability, suitability, accuracy, transparency, interoperability, availability, reliability, stability, cost, throughput, efficiency, and scalability. Customer feedback is also incorporated to assess these trust factors prior to service recommendation. The proposed model calculates service ranking (SR) values for available cloud services and dynamically includes newly introduced services during the validation process by mapping them with existing entries in the Service Collection Repository (SCR). Performance evaluation using the Google cluster-usage traces dataset demonstrates that the ICB outperforms existing approaches such as the Clustering-Based Trust Degree Computation (CBTDC) algorithm and the Service Context-Aware QoS Prediction and Recommendation (SCAQPR) model. Results confirm that the ICB significantly enhances the effectiveness and reliability of cloud service recommendations for users. Full article
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16 pages, 3339 KiB  
Article
Accurate Identification of Native Asian Honey Bee Populations in Jilong (Xizang, China) by Population Genomics and Deep Learning
by Zhiyu Liu, Yongqiang Xu, Wei Sun, Bing Yang, Tenzin Nyima, Zhuoma Pubu, Xin Zhou, Wa Da and Shiqi Luo
Insects 2025, 16(8), 788; https://doi.org/10.3390/insects16080788 (registering DOI) - 31 Jul 2025
Viewed by 217
Abstract
The Jilong Valley, situated in Rikaze, Xizang, China, is characterized by its complex topography and variable climatic conditions, providing a suitable habitat for Apis cerana Fabricius, 1793. To facilitate the conservation of germplasm resources and maintain genetic diversity, it is imperative to elucidate [...] Read more.
The Jilong Valley, situated in Rikaze, Xizang, China, is characterized by its complex topography and variable climatic conditions, providing a suitable habitat for Apis cerana Fabricius, 1793. To facilitate the conservation of germplasm resources and maintain genetic diversity, it is imperative to elucidate the population structure and lineage differentiation of A. cerana within this ecologically distinct region. In this study, we collected A. cerana specimens from 12 geographically disparate locations across various altitudinal gradients within the Jilong Valley, and also integrated publicly available sequencing data of A. cerana from various regions across mainland Asia. In total, our analysis encompassed sequencing data from 296 individuals. Population structure analyses based on SNP data revealed that A. cerana in Jilong represents a genetically distinct population that differs markedly from other regional A. cerana populations in terms of genetic lineage, although its subspecies identity remains to be confirmed. Through screening based on FST values, we identified SNP loci that contribute significantly to distinguishing between Jilong and non-Jilong A. cerana. Using these loci, the convolutional neural network model TraceNet was trained, which demonstrated specific recognition capabilities for Jilong versus non-Jilong A. cerana. This further confirmed the universality and efficiency of TraceNet in identifying honey bee lineages. These findings contribute valuable insights for the identification and conservation of A. cerana germplasm resources in specific geographical regions. Full article
(This article belongs to the Section Insect Systematics, Phylogeny and Evolution)
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15 pages, 857 KiB  
Article
A Pilot Study on the Use of Pumpkin Waste as Cattle Feed
by Minori Nizuka, Hironobu Ishihara, Jun Nakahigashi, Daisaku Matsumoto and Eiji Kobayashi
Metabolites 2025, 15(8), 511; https://doi.org/10.3390/metabo15080511 (registering DOI) - 31 Jul 2025
Viewed by 216
Abstract
Background/Objectives: Pumpkin seed pulp from processing plants offers high nutritional value due to its rich β-carotene content, making it a potential functional feed ingredient. This study investigated the effects of pumpkin seed pulp, which has already been administered as livestock feed, on [...] Read more.
Background/Objectives: Pumpkin seed pulp from processing plants offers high nutritional value due to its rich β-carotene content, making it a potential functional feed ingredient. This study investigated the effects of pumpkin seed pulp, which has already been administered as livestock feed, on key physiological parameters in cattle, including the concentration of β-carotene in the blood measured during routine health monitoring. Methods: Here, pumpkin waste cultivated in various fields was processed into cattle feed (pumpkin seed pulp flakes, PSPFs) by grinding and drying, and residual pesticide (heptachlor) and β-carotene contents were measured. A pilot feeding trial was conducted with 13 cattle (7 in the treatment group and 6 in the control group) and blood component analysis was performed, and findings were contextualized with a literature review. Results: Heptachlor concentrations varied depending on the cultivation site of raw pumpkins. Among the six lots produced using raw materials sourced from fields not contracted by the Air Water Group—a collective of companies in which Air Water Inc. holds more than 51% ownership—three exceeded the regulatory limits for animal feed established in Japan. PSPFs contained high levels of β-carotene, as expected. Blood tests before and after the feeding trial indicated absorption of β-carotene in the cattle. Maintaining high plasma β-carotene concentrations in cattle has been associated with improved immune function and reproductive performance. Conclusions: Our study demonstrates that PSPFs are a promising, environmentally friendly, and natural β-carotene-rich feed ingredient. Tracing the cultivation fields of raw pumpkins can help ensure feed safety. Full article
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20 pages, 3857 KiB  
Review
Utility of Enabling Technologies in Spinal Deformity Surgery: Optimizing Surgical Planning and Intraoperative Execution to Maximize Patient Outcomes
by Nora C. Kim, Eli Johnson, Christopher DeWald, Nathan Lee and Timothy Y. Wang
J. Clin. Med. 2025, 14(15), 5377; https://doi.org/10.3390/jcm14155377 - 30 Jul 2025
Viewed by 289
Abstract
The management of adult spinal deformity (ASD) has evolved dramatically over the past century, transitioning from external bracing and in situ fusion to complex, technology-driven surgical interventions. This review traces the historical development of spinal deformity correction and highlights contemporary enabling technologies that [...] Read more.
The management of adult spinal deformity (ASD) has evolved dramatically over the past century, transitioning from external bracing and in situ fusion to complex, technology-driven surgical interventions. This review traces the historical development of spinal deformity correction and highlights contemporary enabling technologies that are redefining the surgical landscape. Advances in stereoradiographic imaging now allow for precise, low-dose three-dimensional assessment of spinopelvic parameters and segmental bone density, facilitating individualized surgical planning. Robotic assistance and intraoperative navigation improve the accuracy and safety of instrumentation, while patient-specific rods and interbody implants enhance biomechanical conformity and alignment precision. Machine learning and predictive modeling tools have emerged as valuable adjuncts for risk stratification, surgical planning, and outcome forecasting. Minimally invasive deformity correction strategies, including anterior column realignment and circumferential minimally invasive surgery (cMIS), have demonstrated equivalent clinical and radiographic outcomes to traditional open surgery with reduced perioperative morbidity in select patients. Despite these advancements, complications such as proximal junctional kyphosis and failure remain prevalent. Adjunctive strategies—including ligamentous tethering, modified proximal fixation, and vertebral cement augmentation—offer promising preventive potential. Collectively, these innovations signal a paradigm shift toward precision spine surgery, characterized by data-informed decision-making, individualized construct design, and improved patient-centered outcomes in spinal deformity care. Full article
(This article belongs to the Special Issue Clinical New Insights into Management of Scoliosis)
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12 pages, 3234 KiB  
Article
Shooting Activities as the Potential Influence on the Environment at the Pokljuka Biathlon Center, Slovenia
by Valentina Pezdir and Mateja Gosar
Atmosphere 2025, 16(8), 915; https://doi.org/10.3390/atmos16080915 - 29 Jul 2025
Viewed by 157
Abstract
Biathlon activities can have negative effects on the environment due to the processes occurring during shooting, as the biathlon ammunition contains significant amounts of Pb, Cu and Sb. To determine these effects, we looked into the presence of lead-bearing particles in the vicinity [...] Read more.
Biathlon activities can have negative effects on the environment due to the processes occurring during shooting, as the biathlon ammunition contains significant amounts of Pb, Cu and Sb. To determine these effects, we looked into the presence of lead-bearing particles in the vicinity of a shooting range in Rudno polje, Pokljuka, in Slovenia. Particles were collected from snow and later analysed using SEM/EDS, where we focused on the lead-bearing particles. These particles are composed of mainly lead or lead oxides/hydroxides with trace presence of Cu and Sb, both of which are commonly related to ammunition used in biathlon shooting and other shooting activities. To confirm that the particles originated from shooting, we compared them with particles taken from an indoor shooting range as dust and residue in ammunition casings. Lead-bearing particles show morphological changes that are caused by high temperatures and pressures during the shooting process. Full article
(This article belongs to the Section Biosphere/Hydrosphere/Land–Atmosphere Interactions)
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17 pages, 384 KiB  
Article
Reading Between the Lines: Toward a Methodology for Tracing Manichaean Echoes in the Epistulae of Augustine of Hippo
by Marc-Thilo Glowacki and Anthony Dupont
Religions 2025, 16(8), 981; https://doi.org/10.3390/rel16080981 - 29 Jul 2025
Viewed by 235
Abstract
Augustine of Hippo (354–430), one of the most influential theologians of Late Antiquity, spent nearly a decade in the Manichaean sect before becoming a central figure in the shaping of Western “orthodox” Christianity. While his major works such as the Confessiones and De [...] Read more.
Augustine of Hippo (354–430), one of the most influential theologians of Late Antiquity, spent nearly a decade in the Manichaean sect before becoming a central figure in the shaping of Western “orthodox” Christianity. While his major works such as the Confessiones and De civitate Dei have been extensively studied for their treatment of Manichaeism, the vast collection of his ca. 300 preserved letters (Epistulae) remains an understudied source for understanding this aspect of his intellectual and theological development. This article addresses that gap by proposing a methodology to identify both anti- and crypto-Manichaean themes in his letters. Drawing on phenomenological openness, hermeneutical perspective, and close reading, the study also incorporates genuine Manichaean sources and anti-Manichaean polemics to contextualise Augustine’s rhetorical strategies. The Epistulae, unpolished and situated in specific communicative contexts, offer a unique view of Augustine’s doctrinal positioning after his conversion. Traces of his Manichaean past re-emerge in vocabulary, argumentation, and theological emphasis. This is exemplified in Epistula 137 to Volusianus (411–412), which, without naming the sect, covertly critiques key Manichaean doctrines such as Docetism and materialism. These critiques align with extant Manichaean sources and may reflect Augustine’s awareness of latent Manichaean influence in Christian communities. By bringing the Epistulae into the broader discussion of Augustine’s anti-Manichaean engagement, this study highlights their value as a window into his theological evolution and pastoral strategy in a religiously contested environment. Full article
18 pages, 278 KiB  
Review
Biomarkers over Time: From Visual Contrast Sensitivity to Transcriptomics in Differentiating Chronic Inflammatory Response Syndrome and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
by Ming Dooley
Int. J. Mol. Sci. 2025, 26(15), 7284; https://doi.org/10.3390/ijms26157284 - 28 Jul 2025
Viewed by 325
Abstract
Chronic inflammatory response syndrome (CIRS) and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) are debilitating multisystem illnesses that share overlapping symptoms and molecular patterns, including immune dysregulation, mitochondrial impairment, and vascular dysfunction. This review provides a chronological synthesis of biomarker development in CIRS, tracing its [...] Read more.
Chronic inflammatory response syndrome (CIRS) and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) are debilitating multisystem illnesses that share overlapping symptoms and molecular patterns, including immune dysregulation, mitochondrial impairment, and vascular dysfunction. This review provides a chronological synthesis of biomarker development in CIRS, tracing its evolution from early functional tests such as visual contrast sensitivity (VCS) to advanced transcriptomic profiling. Drawing on peer-reviewed studies spanning two decades, we examine the layered integration of neuroendocrine, immunologic, metabolic, and genomic markers that collectively support a multisystem model of innate immune activation specific to environmentally acquired illness. Particular focus is given to the Gene Expression: Inflammation Explained (GENIE) platform’s use of transcriptomics to classify disease stages and distinguish CIRS from other fatiguing conditions. While ME/CFS research continues to explore overlapping pathophysiologic features, it has yet to establish a unified diagnostic model with validated biomarkers or exposure-linked mechanisms. As a result, many patients labeled with ME/CFS may, in fact, represent unrecognized CIRS cases. This review underscores the importance of structured biomarker timelines in improving differential diagnosis and guiding treatment in complex chronic illness and highlights the reproducibility of the CIRS framework in contrast to the diagnostic ambiguity surrounding ME/CFS. Full article
21 pages, 4949 KiB  
Article
An Integrated Lightweight Neural Network Design and FPGA-Accelerated Edge Computing for Chili Pepper Variety and Origin Identification via an E-Nose
by Ziyu Guo, Yong Yin, Haolin Gu, Guihua Peng, Xueya Wang, Ju Chen and Jia Yan
Foods 2025, 14(15), 2612; https://doi.org/10.3390/foods14152612 - 25 Jul 2025
Viewed by 246
Abstract
A chili pepper variety and origin detection system that integrates a field-programmable gate array (FPGA) with an electronic nose (e-nose) is proposed in this paper to address the issues of variety confusion and origin ambiguity in the chili pepper market. The system uses [...] Read more.
A chili pepper variety and origin detection system that integrates a field-programmable gate array (FPGA) with an electronic nose (e-nose) is proposed in this paper to address the issues of variety confusion and origin ambiguity in the chili pepper market. The system uses the AIRSENSE PEN3 e-nose from Germany to collect gas data from thirteen different varieties of chili peppers and two specific varieties of chili peppers originating from seven different regions. Model training is conducted via the proposed lightweight convolutional neural network ChiliPCNN. By combining the strengths of a convolutional neural network (CNN) and a multilayer perceptron (MLP), the ChiliPCNN model achieves an efficient and accurate classification process, requiring only 268 parameters for chili pepper variety identification and 244 parameters for origin tracing, with 364 floating-point operations (FLOPs) and 340 FLOPs, respectively. The experimental results demonstrate that, compared with other advanced deep learning methods, the ChiliPCNN has superior classification performance and good stability. Specifically, ChiliPCNN achieves accuracy rates of 94.62% in chili pepper variety identification and 93.41% in origin tracing tasks involving Jiaoyang No. 6, with accuracy rates reaching as high as 99.07% for Xianjiao No. 301. These results fully validate the effectiveness of the model. To further increase the detection speed of the ChiliPCNN, its acceleration circuit is designed on the Xilinx Zynq7020 FPGA from the United States and optimized via fixed-point arithmetic and loop unrolling strategies. The optimized circuit reduces the latency to 5600 ns and consumes only 1.755 W of power, significantly improving the resource utilization rate and processing speed of the model. This system not only achieves rapid and accurate chili pepper variety and origin detection but also provides an efficient and reliable intelligent agricultural management solution, which is highly important for promoting the development of agricultural automation and intelligence. Full article
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28 pages, 20978 KiB  
Article
From Painting to Cinema: Archetypes of the European Woman as a Cultural Mediator in the Western genre
by Olga Kosachova
Arts 2025, 14(4), 83; https://doi.org/10.3390/arts14040083 - 23 Jul 2025
Viewed by 409
Abstract
The Western genre has traditionally been associated with American identity and male-dominated narratives. However, recent decades have seen increasing attention to female protagonists, particularly the European woman as a cultural mediator within the frontier context. This study aims to identify the archetypes of [...] Read more.
The Western genre has traditionally been associated with American identity and male-dominated narratives. However, recent decades have seen increasing attention to female protagonists, particularly the European woman as a cultural mediator within the frontier context. This study aims to identify the archetypes of the European woman in the Western genre through a diachronic and comparative analysis of the visual language found in European painting from the late 17th to early 19th centuries and in 20th–21st century cinema. The research methodology combines narrative, visual, and semiotic analysis, with a focus on intermedial and intertextual parallels between visual art and film. The study identifies nine archetypal models corresponding to goddesses of the Greek pantheon and traces their transformation across different aesthetic systems. These archetypes, rooted in artistic traditions such as Baroque, Classicism, Romanticism, and others, reappear in Western films through compositional, symbolic, and iconographic strategies, demonstrating their persistence and ability to transcend temporal, medial, and geographical boundaries. The findings suggest that the woman in the Western genre is not merely a central character, but a visual sign that activates cultural memory and engages with deep archetypal structures embedded in the collective unconscious. Full article
(This article belongs to the Special Issue What is ‘Art’ Cinema?)
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23 pages, 2437 KiB  
Article
From Farmworkers to Urban Residents: Mapping Multi-Class Pesticide Exposure Gradients in Morocco via Urinary Biomonitoring
by Zineb Ben Khadda, Andrei-Flavius Radu, Souleiman El Balkhi, Fagroud Mustapha, Yahya El Karmoudi, Gabriela Bungau, Pierre Marquet, Tarik Sqalli Houssaini and Sanae Achour
J. Xenobiot. 2025, 15(4), 120; https://doi.org/10.3390/jox15040120 - 23 Jul 2025
Viewed by 316
Abstract
Pesticide exposure gradients between occupational, para-occupational, and general populations remain poorly characterized in North African agricultural contexts. This study evaluates urinary pesticide levels among farmers, indirectly exposed individuals, and a control group in Morocco’s Fez-Meknes region. A cross-sectional survey measured pesticide concentrations using [...] Read more.
Pesticide exposure gradients between occupational, para-occupational, and general populations remain poorly characterized in North African agricultural contexts. This study evaluates urinary pesticide levels among farmers, indirectly exposed individuals, and a control group in Morocco’s Fez-Meknes region. A cross-sectional survey measured pesticide concentrations using LC-MS/MS in urine samples collected from 154 adults residing in both rural and urban areas. A questionnaire was used to gather information from participants regarding factors that may elevate the risk of pesticide exposure. The results revealed that farmers exhibited the highest concentrations of pesticides in their urine, including compounds classified as Ia/Ib by the World Health Organization. Indirectly exposed individuals showed moderate levels of contamination, with notable detections such as dichlofluanid (22.13 µg/L), while the control group had residual traces of neonicotinoids, notably imidacloprid (2.05 µg/L). Multivariate analyses revealed several sociodemographic factors significantly associated with increased pesticide exposure. The main risk factors identified included low education, residence in an agricultural area, and the consumption of untreated water (wells/rivers). Conversely, wearing personal protective equipment was associated with reduced urinary concentrations. This study highlights intense occupational exposure among farmers, secondary environmental contamination among residents living near treated areas, and the widespread dispersion of pesticide residues into urban areas. Full article
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34 pages, 2648 KiB  
Review
Microfluidic Sensors for Micropollutant Detection in Environmental Matrices: Recent Advances and Prospects
by Mohamed A. A. Abdelhamid, Mi-Ran Ki, Hyo Jik Yoon and Seung Pil Pack
Biosensors 2025, 15(8), 474; https://doi.org/10.3390/bios15080474 - 22 Jul 2025
Viewed by 380
Abstract
The widespread and persistent occurrence of micropollutants—such as pesticides, pharmaceuticals, heavy metals, personal care products, microplastics, and per- and polyfluoroalkyl substances (PFAS)—has emerged as a critical environmental and public health concern, necessitating the development of highly sensitive, selective, and field-deployable detection technologies. Microfluidic [...] Read more.
The widespread and persistent occurrence of micropollutants—such as pesticides, pharmaceuticals, heavy metals, personal care products, microplastics, and per- and polyfluoroalkyl substances (PFAS)—has emerged as a critical environmental and public health concern, necessitating the development of highly sensitive, selective, and field-deployable detection technologies. Microfluidic sensors, including biosensors, have gained prominence as versatile and transformative tools for real-time environmental monitoring, enabling precise and rapid detection of trace-level contaminants in complex environmental matrices. Their miniaturized design, low reagent consumption, and compatibility with portable and smartphone-assisted platforms make them particularly suited for on-site applications. Recent breakthroughs in nanomaterials, synthetic recognition elements (e.g., aptamers and molecularly imprinted polymers), and enzyme-free detection strategies have significantly enhanced the performance of these biosensors in terms of sensitivity, specificity, and multiplexing capabilities. Moreover, the integration of artificial intelligence (AI) and machine learning algorithms into microfluidic platforms has opened new frontiers in data analysis, enabling automated signal processing, anomaly detection, and adaptive calibration for improved diagnostic accuracy and reliability. This review presents a comprehensive overview of cutting-edge microfluidic sensor technologies for micropollutant detection, emphasizing fabrication strategies, sensing mechanisms, and their application across diverse pollutant categories. We also address current challenges, such as device robustness, scalability, and potential signal interference, while highlighting emerging solutions including biodegradable substrates, modular integration, and AI-driven interpretive frameworks. Collectively, these innovations underscore the potential of microfluidic sensors to redefine environmental diagnostics and advance sustainable pollution monitoring and management strategies. Full article
(This article belongs to the Special Issue Biosensors Based on Microfluidic Devices—2nd Edition)
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17 pages, 309 KiB  
Article
Heavy Metals in Leafy Vegetables and Soft Fruits from Allotment Gardens in the Warsaw Agglomeration: Health Risk Assessment
by Jarosław Chmielewski, Elżbieta Wszelaczyńska, Jarosław Pobereżny, Magdalena Florek-Łuszczki and Barbara Gworek
Sustainability 2025, 17(15), 6666; https://doi.org/10.3390/su17156666 - 22 Jul 2025
Viewed by 397
Abstract
Vegetables and fruits grown in urban areas pose a potential threat to human health due to contamination with heavy metals (HMs). This study aimed to identify and quantify the concentrations of heavy metals (Fe, Mn, Zn, Cu, Pb, Cd) in tomatoes, leafy vegetables, [...] Read more.
Vegetables and fruits grown in urban areas pose a potential threat to human health due to contamination with heavy metals (HMs). This study aimed to identify and quantify the concentrations of heavy metals (Fe, Mn, Zn, Cu, Pb, Cd) in tomatoes, leafy vegetables, and fruits collected from 16 allotment gardens (AGs) located in Warsaw. A total of 112 samples were analyzed (72 vegetable and 40 fruit samples). Vegetables from AGs accumulated significantly higher levels of HMs than fruits. Leafy vegetables, particularly those cultivated near high-traffic roads, exhibited markedly elevated levels of Pb, Cd, and Zn compared to those grown in peripheral areas. Lead concentrations exceeded permissible limits by six to twelve times, cadmium by one to thirteen times, and zinc by 0.7 to 2.4 times. Due to high levels of Pb and Cd, tomatoes should not be cultivated in urban environments. Regardless of location, only trace amounts of HMs were detected in fruits. The greatest health risk is associated with the consumption of leafy vegetables. Lettuce should be considered an indicator plant for assessing environmental contamination. The obtained Hazard Index (HI) values indicate that only the tested fruits are safe for consumption. Meanwhile, the values of the Hazard Quotient (HQ) indicate no health risk associated with the consumption of lettuce, cherries, and red currants. Among the analyzed elements, Pb showed a higher potential health risk than other metals. This study emphasizes the need for continuous monitoring of HM levels in urban soils and the establishment of baseline values for public health purposes. Remediation of contaminated soils and the implementation of safer agricultural practices are recommended to reduce the exposure of urban populations to the risks associated with the consumption of contaminated produce. In addition, the safety of fruits and vegetables grown in urban areas is influenced by the location of the AGs and the level of industrialization of the agglomeration. Therefore, the safety assessment of plant products derived from AGs should be monitored on a continuous basis, especially in vegetables. Full article
(This article belongs to the Special Issue Soil Microorganisms, Plant Ecology and Sustainable Restoration)
16 pages, 2557 KiB  
Article
Surveillance of Salmonella Serovars in the Food Chain in Poland: A Five-Year Review (2016–2020)
by Ewelina Skrzypiec, Magdalena Skarżyńska, Magdalena Zając, Renata Kwit, Anna Lalak, Aleksandra Śmiałowska-Węglińska, Emilia Mikos-Wojewoda, Paulina Pasim, Weronika Koza, Dominika Wojdat, Inga Bona, Dominika Pastuszka, Sylwia Hudzik-Pałosz and Dariusz Wasyl
Pathogens 2025, 14(7), 712; https://doi.org/10.3390/pathogens14070712 - 18 Jul 2025
Viewed by 289
Abstract
(1) Background: Understanding the distribution of Salmonella serovars in food, animals, and their environments is crucial for identifying infection sources and monitoring pathogen prevalence in the food chain. This study analysed Salmonella serovars in Poland from 2016 to 2020, focusing on their epidemiological [...] Read more.
(1) Background: Understanding the distribution of Salmonella serovars in food, animals, and their environments is crucial for identifying infection sources and monitoring pathogen prevalence in the food chain. This study analysed Salmonella serovars in Poland from 2016 to 2020, focusing on their epidemiological significance. (2) Methods: Isolation of Salmonella was carried out following PN-EN ISO 6579 standards, and serotyping was performed using the White–Kauffmann–Le Minor scheme. A total of 7104 isolates were collected from food-producing animals, their environments, food of animal origin, feedingstuffs, and fertilisers. (3) Results: A total of 175 serovars were identified, with S. Enteritidis (n = 2905; 40.9%), S. Infantis (n = 1167; 16.4%), and S. Typhimurium (n = 360; 5.1%) being the most prevalent. Species-specific patterns were observed: S. Enteritidis dominated in chickens, ducks, and cattle; S. Kentucky in turkeys; S. Typhimurium in geese; and monophasic S. Typhimurium in pigs. S. Enteritidis and S. Infantis were most frequent in food of animal origin, especially broiler meat. In feedingstuffs, S. Agona was predominant, while fertilisers mostly contained S. Derby and S. Infantis. (4) Conclusions: The study highlights the source-dependent variety of Salmonella serovars and the importance of serotyping in tracing infection routes and preventing the spread of pathogens. Identifying the most common serovars supports the development of targeted preventive measures, including improved biosecurity, hygiene, and management practices to enhance food safety. Full article
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