Search Results (2,885)

Reconstructing original cusp dimensions in worn molars represents a fundamental challenge across dentistry, anthropology, and paleontology, as dental wear obscures critical morphological information. In this proof-of-concept study, we present a standardized machine learning pipeline for predicting original cusp height, specifically the horn tips of the enamel–dentine junction (EDJ), in worn lower molars using three-dimensional morphometric data from micro-computed tomography (micro-CT). We analyzed 40 permanent lower first (M1) and second (M2) molars from four hominin groups, systematically evaluated across three wear stages: original, moderately worn (worn1), and severely worn (worn2). Morphometric variables including height, area, and volume were quantified for each cusp, with Random Forest and multiple linear regression models developed individually and combined through ensemble methods. To mimic realistic reconstruction scenarios while preserving a known ground truth, models were trained on unworn specimens (original EDJ morphology) and tested on other teeth after digitally simulated wear (worn1 and worn2). Predictive performance was evaluated using root mean square error (RMSE) and coefficient of determination (R²). Our results demonstrate that under moderate wear (worn1), the ensemble models achieved normalized RMSE values between 11% and 17%. Absolute errors typically below 0.25 mm for most cusps, with R² values up to ~0.69. Performance deteriorated under severe wear (worn2), particularly for morphologically variable cusps such as the hypoconid and entoconid, but generally remained within sub-millimetric error ranges for several structures. Random Forests and linear models showed complementary strengths, and the ensemble generally offered the most stable performance across cusps and wear states. To enhance transparency and accessibility, we provide a comprehensive, user-friendly software pipeline including pre-trained models, automated prediction scripts, standardized data templates, and detailed documentation. This implementation allows researchers without advanced machine learning expertise to explore EDJ-based reconstruction from standard morphometric measurements in new datasets, while explicitly acknowledging the limitations imposed by our modest and taxonomically unbalanced sample. More broadly, the framework represents an initial step toward predicting complete crown morphology, including enamel thickness, in worn or damaged teeth. As such, it offers a validated methodological foundation for future developments in cusp and crown reconstruction in both clinical and evolutionary dental research. Full article

In the diagnostics of plasmas heated by neutral beam injection (NBI), which serves as a fundamental heating technique, critical core parameters such as ion temperatures and rotational velocities can be measured through NBI’s associated diagnostic methods. However, conventional spectral analysis methods applied in NBI-based Beam Emission Spectroscopy diagnostics face a significant limitation: a relatively high false detection rate during characteristic peak detection and boundary determination. This issue stems from three primary factors: persistent noise interference, overlapping spectral peaks, and dynamic broadening effects. To address this critical issue, we propose a spectral feature extraction method based on morphological segmentation and optimized clustering, with three key innovations that work synergistically: (1) an adaptive chunking algorithm driven by gradient, Laplacian, and curvature features to dynamically partition spectral regions, laying a foundation for localized analysis; (2) a hierarchical residual iteration mechanism combining dynamic thresholding and Gaussian template subtraction to enhance weak peak signals; (3) optimized DBSCAN clustering integrated with morphological closure to refine peak boundaries accurately. Among them, the adaptive chunking technique is distinct from general adaptive methods: its chunking granularity can be dynamically adjusted according to peak structures and can accurately adapt to low signal-to-noise ratio (SNR) scenarios. Experimental results based on measured data from the EAST device demonstrate that the adaptive chunking strategy maintains a missed detection rate of 0–20% across the full signal-to-noise ratio (SNR) range, with false positive rates limited to 16.67–50.00%. Notably, it achieves effective peak detection even under extremely low SNR conditions. Full article

Digital elevation models (DEMs) play a critical role in geospatial analysis and surface modeling. However, due to differences in data collection payload, data processing methodology, and data reference baseline, DEMs acquired from various sources often exhibit systematic spatial offsets. This limitation substantially constrains their accuracy and reliability in multi-source joint analysis and fusion applications. Traditional registration methods such as the Least-Z Difference (LZD) method are sensitive to gross errors, while multimodal registration approaches overlook the importance of elevation information. To address these challenges, this paper proposes a DEM registration method based on physically consistent rendering and multimodal image matching. The approach converts DEMs into image data through irradiance-based models and parallax geometric models. Feature point pairs are extracted using template-based matching techniques and further refined through elevation consistency analysis. Reliable correspondences are selected by jointly considering elevation error distributions and geometric consistency constraints, enabling robust affine transformation estimation and elevation bias correction. The experimental results demonstrate that in typical terrains such as urban areas, glaciers, and plains, the proposed method outperforms classical DEM registration algorithms and state-of-the-art remote sensing image registration algorithms. The results indicate clear advantages in registration accuracy, robustness, and adaptability to diverse terrain conditions, highlighting the potential of the proposed framework as a universal DEM collaborative registration solution. Full article

Candida albicans is a major fungal pathogen associated with vulvovaginal candidiasis, and rapid, sensitive detection remains challenging, particularly in amplification-free formats. Here, we report NaPddCas, a microfluidic-free, droplet-based CRISPR/Cas12a detection strategy for qualitative identification of Candida albicans DNA. Unlike conventional bulk CRISPR assays, NaPddCas partitions the reaction mixture into vortex-generated polydisperse droplets, enabling spatial confinement of Cas12a activation events and effective suppression of background fluorescence. This compartmentalization substantially enhances detection sensitivity without nucleic acid amplification or microfluidic devices. Using plasmid and genomic DNA templates, NaPddCas achieved reliable detection at concentrations several orders of magnitude lower than bulk CRISPR/Cas12a reactions. The assay further demonstrated high specificity against non-target bacterial and fungal species and was successfully applied to clinical vaginal secretion samples. Importantly, NaPddCas is designed as a qualitative or semi-qualitative droplet-dependent digital detection method rather than a quantitative digital assay. Owing to its simplicity, sensitivity, and amplification-free workflow, NaPddCas represents a practical approach for laboratory-based screening of Candida albicans infections. Full article

Background/Objectives: Lithium (Li), silicon (Si), and boron (B) are proposed nutritional trace elements with potential roles in metabolic, neurobiological, endocrine, inflammatory, and bone-related processes. This review provides a critical synthesis of data on Li–Si–B, emphasizing (i) physiological and mechanistic pathways, (ii) human clinical relevance, (iii) shared biological domains, and (iv) safety considerations. Methods: A narrative review was conducted across PubMed, Scopus, and Web of Science from inception to January 2025. Predefined search strings targeted dietary, environmental, and supplemental exposures of lithium, silicon, or boron in relation to metabolism, endocrine function, neurobiology, inflammation, bone health, and the gut microbiome. Inclusion criteria required peer-reviewed studies in English. Data extraction followed a structured template, and evidence was stratified into human, animal, cellular, and ecological tiers. Methodological limitations were critically appraised. Results: Li, Si, and B influence overlapping molecular pathways including oxidative stress modulation, mitochondrial stability, inflammatory signaling, endocrine regulation, and epithelial/gut barrier function. Human evidence remains limited: Li is supported primarily by small trials; Si by bone-related observational studies and biomarker-oriented interventions; and B by metabolic, inflammatory, and cognitive studies of modest sample size. Convergence across elements appears in redox control, barrier function, and neuroimmune interactions, but mechanistic synergism remains hypothetical. Conclusions: Although Li–Si–B display compelling mechanistic potential, current human data are insufficient to justify dietary recommendations or supplementation. Considerable research gaps—including exposure assessment, dose–response characterization, toxicity thresholds, and controlled human trials—must be addressed before translation into public health policy. Full article

Porous NiCo₂O₄ nanomaterials were synthesized using in situ-generated polyacrylamide as a template, with cobalt nitrate, nickel nitrate, and urea serving as raw materials. XRD and FESEM analyses confirm the successful formation of spinel-structured NiCo₂O₄ electrode materials featuring a 3D macroporous/mesoporous architecture and an average crystalline size of approximately 8.1 nm, obtained through calcination of the amorphous precursor. Electrochemical evaluation of the as-prepared NiCo₂O₄ reveals that the specific capacitance retained at 10 A g⁻¹ reaches 88.9% of the value measured at 1 A g⁻¹, demonstrating excellent rate capability. Furthermore, the material exhibits a gradual increase in specific capacity over 3000 charge–discharge cycles, achieving a capacitance retention of up to 246.5%, which indicates good cycling stability and superior capacity retention. Full article

The rapid evolution of coronaviruses (CoVs) requires researchers to develop specific yet broad-spectrum detection methods to monitor their constant genomic changes. The goal of the present study was to establish a current pan-coronavirus RT-PCR system capable of detecting a wide variety of CoVs and useful for the investigation of virus diversity and host spectrum. For optimization, one-step and two-step nested RT-PCRs with three RT enzymes were examined, amplifying a ~600 bp long product of the RNA-dependent RNA polymerase. As templates, the in vitro transcribed RNA of ten pathogenic CoVs (SARS-CoV, SARS-CoV-2, NL-63, OC43, feline CoV, porcine epidemic diarrhea virus or PEDV, transmissible gastroenteritis virus or TGEV, canine CoV, bat CoV, and infectious bronchitis virus) were applied instead of the often-used DNA standards. A limit of detection of 5–50 copies/reaction was achieved with a random hexamer-primed two-step RT-PCR and a touchdown cycling profile, representing a lower detection limit and higher specificity compared to previously published primer sets. Swine origin pooled samples (n = 121), collected from apparently healthy herds in Hungary, were tested with the novel RT-PCR system. Sequences of porcine respiratory CoV/TGEV and porcine hemagglutinating encephalomyelitis virus were identified in 24 oral fluid and nasal swab pools, demonstrating the circulation of these viruses in this country, as well as the suitability of the new PCR for their detection. The results highlighted the importance of adequate RT enzyme selection and the use of RNase inhibitors in sample preparation and conservation. Full article

As an emerging threat to global food security, wheat blast necessitates the development of a rapid and field-deployable detection system to facilitate early diagnosis, enable effective management, and prevent its further spread to new regions. In this study, we aimed to validate and improve a Recombinase Polymerase Amplification coupled with PCRD lateral flow detection (RPA-PCRD strip assay) kit for the rapid and specific identification of Magnaporthe oryzae pathotype Triticum (MoT) in field samples. The assay demonstrated exceptional sensitivity, detecting as low as 10 pg/µL of target DNA, and exhibited no cross-reactivity with M. oryzae Oryzae (MoO) isolates and other major fungal phytopathogens under the genera of Fusarium, Bipolaris, Colletotrichum, and Botrydiplodia. The method successfully detected MoT in wheat leaves as early as 4 days post-infection (DPI), and in infected spikes, seeds, and alternate hosts. Furthermore, by combining a simplified polyethylene glycol-NaOH method for extracting DNA from plant samples, the entire RPA-PCRD strip assay enabled the detection of MoT within 30 min with no specialized equipment and high technical skills at ambient temperature (37–39 °C). When applied to field samples, it successfully detected MoT in naturally infected diseased wheat plants from seven different fields in a wheat blast hotspot district, Meherpur, Bangladesh. Training 52 diverse stakeholders validated the kit’s field readiness, with 88% of trainees endorsing its user-friendly design. This method offers a practical, low-cost, and portable point-of-care diagnostic tool suitable for on-site genomic surveillance, integrated management, seed health testing, and quarantine screening of wheat blast in resource-limited settings. Furthermore, the RPA-PCRD platform serves as an early warning modular diagnostic template that can be readily adapted to detect a wide array of phytopathogens by integrating target-specific genomic primers. Full article

Traditional scoring of makeup face templates in beauty skill assessments heavily relies on manual judgment, leading to inconsistencies and subjective bias. Hand-drawn templates often exhibit proportion distortions, asymmetry, and occlusions that reduce the accuracy of conventional facial landmark detection algorithms. This study proposes a novel approach that integrates Geometric Feature Enhancement (GFE) with Dlib’s 68-landmark detection to improve the robustness and precision of landmark localization. A comprehensive comparison among Haar Cascade, MTCNN-MobileNetV2, and Dlib was conducted using a curated dataset of 11,600 hand-drawn facial templates. The proposed GFE-enhanced Dlib achieved 60.5% accuracy—outperforming MTCNN (23.4%) and Haar (20.3%) by approximately 37 percentage points, with precision and F1-score improvements exceeding 20% and 25%, respectively. The results demonstrate that the proposed method significantly enhances detection accuracy and scoring consistency, providing a reliable framework for automated beauty skill evaluation, and laying a solid foundation for future applications such as digital archiving and style-guided synthesis. Full article

Engineered skin (ES) can serve as an advanced therapy for treatment of large full-thickness wounds, but delayed vascularization can cause ischemia, necrosis, and graft failure. To accelerate ES vascularization, this study assessed incorporation of polydopamine (PDA) microparticles loaded with different concentrations of basic fibroblast growth factor (bFGF) into collagen scaffolds, which were subsequently seeded with human fibroblasts to create dermal templates (DTs), and then keratinocytes to create ES. DTs and ES were evaluated in vitro and following grafting to full-thickness wounds in immunodeficient mice. In vitro, metabolic activity of DTs was enhanced with PDA+bFGF, though this increase was not observed following seeding with keratinocytes to generate ES. After grafting, ES with bFGF-loaded PDA microparticles displayed dose-dependent increases in CD31-positive vessel formation vs. PDA-only controls (p < 0.001 at day 7; p < 0.05 at day 14). Interestingly, ES containing PDA+bFGF microparticles exhibited an almost 3-fold increase in water loss through the skin and a less-organized basal keratinocyte layer at day 14 post-grafting vs. controls. This was associated with significantly increased inflammatory cell infiltrate vs. controls at day 7 in vivo (p < 0.001). The results demonstrate that PDA microparticles are a viable method for delivery of growth factors in ES. However, further investigation of bFGF concentrations, and/or investigation of alternative growth factors, will be required to promote vascularization while reducing inflammation and maintaining epidermal health. Full article

Security design for large-scale infrastructure systems requires substantial effort and often causes development delays. In line with NIST guidance, such systems should consider security design throughout a system development lifecycle. Nevertheless, performing security design in early phases of the lifecycle is difficult due to frequent specification changes and variability in analyst expertise, which causes repeated rework. The workload is particularly critical in threat analysis, the key activity of security design, because rework can inflate the workload. To address this challenge, we propose an automated threat-analysis method. Specifically, (i) we systematize past security design cases and develop “templates” that organize the system-configuration and security information required for threat analysis into a reusable 5W-based format (When, Where, Who, Why, What); (ii) we define dependencies among the templates and design an algorithm that automatically generates threat-analysis results; and (iii) observing that threat analysis of large-scale systems often yield overlaps, we introduce “business operations” as an analytical asset, which includes encompassing information, function, and physical resources. We apply our method to an actual large-scale operational system and confirm that it reduces the workload by up to 84% relative to conventional manual analysis, while maintaining both the coverage and the accuracy of the analysis. Full article

Predicting the severity of traffic accidents remains challenging due to the limited ability of existing methods to extract deep semantic information from unstructured accident narratives, as traditional approaches typically depend on structured data alone. This study proposes a severity prediction approach enhanced by semantic risk reasoning derived from large language models (LLMs). A prompt-engineering template is designed to guide LLMs in extracting proxy semantic features from accident descriptions, forming an enriched feature set that incorporates causal logic. These semantic features are fused with traditional structured features through three integration strategies—direct feature concatenation, optimized feature selection, and model-level fusion. Experiments based on 4013 accident records from expressways in Yunnan Province, China, demonstrate that models using LLM-derived semantic features significantly outperform those relying solely on structured features. Notably, the LightGBM model utilizing semantic features within a balanced learning framework achieves a severe accident recall of 77.8%. While model-level fusion proves optimal for XGBoost (improving Macro-F1 to 0.6356), we identify a “feature dilution” effect in other classifiers, where high-quality semantic reasoning is compromised by low-quality structured noise. These findings indicate that the proposed approach effectively enhances the identification of high-risk accidents and offers a novel semantic-aware solution for traffic safety management. Furthermore, the obtained results provide actionable insights for traffic management agencies to optimize emergency response resource allocation and formulate targeted accident prevention strategies. Full article

Background: Accurate assessment of prolonged disorders of consciousness (pDOC) is a critical clinical challenge. Misdiagnosis in pDOC can occur in up to 40% of cases, highlighting the need for more objective and reproducible biomarkers to support neurophysiological scales, thereby improving diagnosis and guiding therapeutic and prognostic decisions. Electroencephalography (EEG) microstate analysis is a promising, non-invasive method for tracking large-scale brain dynamics, but research in pDOC has predominantly relied on a canonical 4-class model. This methodological constraint may limit the ability to capture the full complexity of neural alterations present in these patients. Objective: This pilot study aimed to offer an objective method for assessing consciousness, complementing and enhancing the existing approaches established in the literature. The classical 4-class and an extended 7-class microstate model were compared to determine which more accurately characterizes the complexity of resting-state brain dynamics across different levels of consciousness in pDOC patients and healthy controls (HCs). Methods: Retrospective resting-state EEG (rsEEG) data from a cohort of pDOC patients and HC subjects were analyzed. Microstate analysis was performed using both 4-class and 7-class templates. The models were evaluated and compared based on three criteria: spatial correspondence with canonical maps (shared variance), the number of significant intra-group correlations between temporal features (Spearman test), and their ability to discriminate between the pDOC and HC groups (Wilcoxon test). Results: The 7-class microstate model provided a more accurate description of brain activity for most participants, with a greater number of microstate classes exceeding the 50% shared variance threshold compared to the 4-class model. In the pDOC group, both the 4-class and 7-class models showed a mean shared variance <50% in class D, which is associated with executive functioning across both templates. For the HC group, a prevalence of classes B and D emerged in both models, indicating higher engagement of executive functions. Furthermore, the 7-class model allowed for a group-specific analysis, which demonstrated that microstates A and F were consistently shared among 86% of pDOC patients. This suggests the potential preservation of specific intrinsic brain networks, particularly the sensory and default networks, even in the presence of severely impaired consciousness. Moreover, the 7-class model yielded a higher number of significant correlations within both groups and identified a broader set of temporal features that were significantly different between pDOC patients and HCs. These results highlight the enhanced sensitivity of the 7-class model in distinguishing subtle brain dynamics and improving the diagnostic capability for pDOC. Conclusions: The 7-class microstate model provides a more fine-grained and sensitive characterization of brain activity in both pDOC patients and healthy individuals. It demonstrated better performance in capturing individual brain dynamics, identifying shared network patterns, and discriminating between clinical populations. These findings suggest that the extended 7-class model holds greater potential for clinical utility and could lead to the development of more robust biomarkers for assessing consciousness. Full article

Introduction: Nursing educators and clinical leaders face persistent challenges in engaging the next generation of nurses, often characterized by short attention spans, frequent phone use, and underdeveloped communication skills. This article describes the design and delivery of a full-day interactive teaching workshop for nursing faculty, senior clinical nurses, and nurse leaders, developed using a design-thinking approach supported by generative AI. Methods: The workshop comprised four thematic sessions: (1) Learning styles across generations, (2) Interactive teaching methods, (3) Application of interactive teaching strategies, and (4) Lesson planning and transfer. Generative AI was used during planning to create icebreakers, discussion prompts, clinical teaching scenarios, and application templates. Design decisions emphasized low-tech, low-prep strategies suitable for spontaneous clinical teaching, thereby reducing barriers to adoption. Activities included emoji-card introductions, quick generational polls, colored-paper reflections, portable whiteboard brainstorming, role plays, fishbowl discussions, gallery walks, and movement-based group exercises. Participants (N = 37) were predominantly female (95%) and represented multiple generations of X, Y, and Z. Mid- and end-of-workshop reflection prompts were embedded within Sessions 2 and 4, with participants recording their responses on colored papers, which were then compiled into a single Word document for thematic analysis. Results: Thematic analysis of 59 mid- and end-workshop reflections revealed six interconnected themes, grouped into three categories: (1) engagement and experiential learning, (2) practical applicability and generational awareness, and (3) facilitation, environment, and motivation. Participants emphasized the workshop’s lively pace and hands-on design. Experiencing strategies firsthand built confidence for application, while generational awareness encouraged reflection on adapting methods for younger learners. The facilitator’s passion, personable approach, and structured use of peer learning created a psychologically safe and motivating climate, leaving participants recharged and inspired to integrate interactive methods. Discussion: The workshop illustrates how AI-assisted, design-thinking-driven professional development can model effective strategies for next-generation learners. When paired with skilled facilitation, AI-supported planning enhances engagement, fosters reflective practice, and promotes immediate transfer of interactive strategies into diverse teaching settings. Full article

Background/Objectives: Obstructive sleep apnea (OSA) affects approximately 1 billion adults worldwide with extensive comorbidities, including cardiovascular disease, metabolic disorders, and cognitive decline, yet pharmacological therapies remain limited. Conventional bottom-up omics approaches identify numerous genes overlapping with other diseases, hindering therapeutic translation. This study introduces a top-down, comorbidity-driven approach to identify actionable molecular targets and develop rational dual kinase inhibitors for OSA management. Methods: We implemented a five-tier modeling workflow: (1) comorbidity network analysis, (2) disease module identification through NetworkAnalyst, (3) mechanistic pathway reconstruction of the CK1δ-(HIF1A)-PINK1 signaling cascade, (4) molecular docking analysis of Nigella sativa alkaloids and reference inhibitors (IC261, PF-670462) against CK1δ (PDB: 3UYS) and PINK1 (PDB: 5OAT) using AutoDock Vina, and (5) rational design and computational validation of novel dual inhibitors (ICL, PFL) integrating pharmacophoric features from natural alkaloids and established kinase inhibitors. Results: Extensive network analysis revealed a discrete OSA disease module centered on two interconnected protein kinases—CK1δ and PINK1—that mechanistically bridge circadian disruption and neurodegeneration. Among natural alkaloids, Nigellidine showed strongest CK1δ binding (−8.0 kcal/mol) and Nigellicine strongest PINK1 binding (−8.6 kcal/mol). Rationally designed dual inhibitors demonstrated superior binding: ICL (−7.2 kcal/mol PINK1, −8.9 kcal/mol CK1δ) and PFL (−10.8 kcal/mol CK1δ, −11.2 kcal/mol PINK1), representing −2.6–2.8 kcal/mol improvements over reference compounds. Conclusions: This study establishes a comorbidity-driven translational framework identifying the CK1δ-PINK1 axis as a therapeutic target in OSA. The rationally designed dual inhibitors represent third-generation precision therapeutics addressing OSA’s multi-dimensional pathophysiology, while the five-tier workflow provides a generalizable template for drug discovery in complex multimorbid diseases. Full article