Search Results (2,692)

Background/Objectives: To investigate whether chronic cosmetics use near or directly on the eyelid margin contributes to tear film instability and meibomian gland dysfunction. Methods: Subjects were enrolled in one of three groups: those who rarely wear makeup (No-M), those who wear it frequently but only outside the eyelid margin (Min-M), and those who wear it frequently and directly on the eyelid margin (W-M). Subjects were assessed for dry eye signs and symptoms by a masked examiner. Lipid layer thickness (LLT), tear meniscus height, meibomian gland excreta grade, number of glands secreting, corneal and conjunctival staining and tear breakup time were assessed. Results: 10 No-M, 18 Min-M, and 21 W-M subjects completed the study. Average fluorescein breakup time was 4.6 s in each group (p = 0.839, 1-way ANOVA). There were higher scores (worse findings) in the marginal eyeliner sample for symptoms (modified Schein, OSDI, SPEED), Oxford and total NEI staining and lower lid meibomian secretions. The W-M group demonstrated a statistically significant increase in the meibomian gland excreta grade (a worsening) compared to the No-M group (mean grades 1.2 and 0.55 respectively; Tukey test, adjusted p < 0.05, 95% CI 0.055–1.187). LLT, tear breakup time, eyelid marginal signs, and meibomian gland dropout had no differences among groups. Conclusions: Eyeliner wear both outside and on the eyelid margin demonstrated increased ocular staining and decreased gland excretion quality, compared to non-makeup users. The meibomian gland excreta decrement may lead to worsening meibomian gland function and potentially glandular atrophy over time. Full article

To address the limitation of insufficient mechanical strength and short service life in biodegradable bamboo fiber mulch film (BFM) replacing plastic film in agriculture, this study applied a biochemical method to make bamboo fiber and used bacterial cellulose (BC) as a natural nanoscale reinforcing agent to fabricate high-performance bacterial cellulose bamboo fiber mulch film (BC-BFM). The physical and mechanical properties, chemical structure, seed germination and degradation behavior performance of BC-BFM were characterized. Results demonstrated the structural compactness and homogeneity of the BC-BFM were improved markedly with the increase in BC addition and BC formed a 3D nanofibrillar network that effectively bridged inter-fiber voids. The tensile, burst and tear indexes of BC-BFM all significantly rose with BC addition. Notably, compared to plastic film and BFM, BC-BFM exhibited a good effect on mung bean seed germination and the best growth speed was at 5% BC addition. Furthermore, the degradation test showed that the degradation rate of BC-BFM within 90 d was three times less than that of BFM and service life was similar to plastic film. This showed that it was a promising method to prepare biodegradable high-quality BFM through biochemical preparation of bamboo fiber and BC nanocellulose reinforcement. This method markedly enhanced the mechanical performance and durability of BC-BFM, providing a feasible technical path for the development of biodegradable high-performance green agricultural covering materials with long service life. Full article

Dry eye disease (DED) is a complex, multifactorial, progressive disease that has consequences both for individuals and society. Symptoms reported by patients include discomfort in the eye and periodic blurred vision, while in the broader perspective, the disease is associated with economic burdens and challenges for healthcare systems. Globally, dry eye disease remains a growing problem observed in many countries. It is estimated that symptoms of dry eye syndrome occur in approximately 10 to 20 per cent of people over the age of 40. This prevalence is on the rise, which is associated with both the aging population and increased incidence among younger adults. In this group, factors such as contact lens wear and prolonged use of digital devices are considered to be contributing factors. Further epidemiological studies, conducted in different regions of the world, covering diverse populations and a wide range of age groups, with a particular focus on younger cohorts, may contribute to a more accurate understanding of the prevalence of dry eye disease. There are more and more methods of diagnosing DED. In addition to well-known procedures like the Schirmer test or tear break-up time, there are also methods that focus on the evaluation of the tear film or imaging of the ocular surface. Moreover, usage of artificial intelligence is also playing a significant role in it. However, the key issue in individual cases is introducing the most effective treatment based on combining available substances, including corticosteroids, antibiotics and supplements, which leads to a reduction in inflammation and improvement in visual comfort. Full article

The in vivo effect of platelet-rich plasma (PRP) on supraspinatus tendon morphology and subacromial bursa cell gene expression in degenerative rotator cuff tears remains unclear. This randomized controlled trial evaluated the effect of preoperative leukocyte-poor PRP (LP-PRP) subacromial injection on supraspinatus tendon histology and subacromial bursa gene expression. Sixteen patients with full-thickness supraspinatus tears were randomized to receive an ultrasound-guided LP-PRP injection (n = 8) or no injection (n = 8) six weeks before arthroscopic repair. Tendon biopsies were assessed using the modified Movin score. Gene expression of collagen type I, II and III, metalloproteinase 3 and 13, and interleukin 1β and 6 genes from subacromial bursa cells was quantified using quantitative real-time PCR. The results of the two groups were compared to determine any statistically significant difference regarding all the examined parameters. The PRP group demonstrated a significantly lower total modified Movin score than controls (6.5 vs. 12.1, p = 0.002), with lower scores for fiber structure, fiber arrangement, nuclear rounding, inflammation and cell density (all p < 0.003), while angiogenesis did not differ (p = 0.149), indicating an architecture closer to that of normal tendon. No statistically significant differences in gene expression were observed (all p > 0.05), although collagen II and metalloproteinase 3 and 13 showed biologically relevant downregulation [fold change 0.23 (95%CI 0.05–1.09), 0.24 (95%CI 0.002–26.10), and 0.26 (95%CI 0.02–2.76), respectively]. The LP-PRP injection was associated with improved supraspinatus tendon histological characteristics and biologically relevant reductions in selected bursal genes, in the setting of supraspinatus tendon tear. Full article

Background/Objectives: The neonatal Fc receptor (FcRn) plays a crucial role in extending the systemic half-life of monoclonal antibodies (mAbs), but its influence on ocular distribution remains incompletely understood. This study investigated the impact of FcRn on the ocular disposition of mAbs following systemic administration in rabbits. Methods: New Zealand White rabbits received a single intravenous dose (1 mg/kg) of either wild-type trastuzumab (TS-WT) or its FcRn non-binding variant (IHH). Plasma and ocular tissues (retina, iris–ciliary body, vitreous humor, aqueous humor, cornea, conjunctiva, and tears) were collected at terminal time points up to 336 h for TS-WT and 168 h for IHH. Antibody concentrations were quantified using a validated sandwich ELISA. Pharmacokinetic parameters and antibody biodistribution coefficients (ABC) were calculated to assess the FcRn-mediated effects on ocular distribution. Results: TS-WT demonstrated 2-fold higher systemic exposure compared to IHH. The iris–ciliary body exhibited the highest absolute exposure for both antibodies, with TS-WT showing significantly higher accumulation (ABC_0–168h: 14.95% vs. 8.89%). Retinal distribution remained comparable between antibodies (5.96% vs. 5.51%). Both antibodies were detectable in tears, with ABC value of ~4% reported for TS-WT. TS-WT also demonstrated markedly increased distribution in vitreous humor and tear fluid (3.5- and 5.5-fold higher ABC values, respectively) compared to IHH. The cornea (5.76% vs. 5.57%) and conjunctiva (7.71% vs. 7.21%) showed comparable relative distribution between TS-WT and IHH, while aqueous humor showed minimal differences (0.44% vs. 0.52%). Conclusions: This investigation reveals distinct tissue-specific patterns of FcRn-mediated mAb distribution within the eye. FcRn binding significantly enhanced antibody distribution in ocular tissues, such as the iris–ciliary body, and tears, with less pronounced effects on the retina, cornea, conjunctiva and aqueous humor. These findings provide mechanistic insights for optimizing mAb-based therapeutics for ocular disease and understanding the ocular toxicity of mAb-based therapeutics, such as antibody–drug conjugates. Full article

Death is a universal phenomenon, defined by the rituals associated with it and conducted within the confines of the culture to which the deceased belonged; thus, it is nearly impossible to universalise a particular mourning pattern, as African people display considerable diversity. The complexities introduced into African mourning by colonialism and other developmental changes, such as urbanisation and the recent COVID-19 pandemic, have relegated many African traditions in relation to bereavement to the periphery. A newer and controversial practice associated with funerals conducted among black communities in South Africa is that of the “after tears” party. Although this practice has not yet infiltrated the rural areas, it is nevertheless a phenomenon that is worth investigating, seeing that those who participate in this practice are predominantly from townships in Gauteng, and such events are gradually occurring in the rural villages as well. Full article

Background/Objectives: Rotator cuff repair (RCR) is a common orthopedic procedure, with healing outcomes strongly influenced by patient-specific factors such as tissue quality, tear characteristics, and biological healing potential. Bioinductive collagen implants have shown great results in enhancing tendon healing and reducing retear rate. This study aimed to evaluate the clinical and imaging outcomes of RCR augmented with a xeno-derived collagen membrane over 24 months and to assess complications or implant failures. Methods: Patients underwent arthroscopic RCR using anchors (single or double-row) with additional xeno-derived matrix augmentation. The study included patients older than 40 years with full-thickness supraspinatus and/or infraspinatus tendon tears (DeOrio–Cofield grade 3–4) who were candidates for arthroscopic rotator cuff repair and provided informed consent. Clinical outcomes were assessed using the Constant–Murley Score (CMS), Disabilities of the Arm, Shoulder and Hand score (DASH), and Visual Analogue Score (VAS) at baseline, 3, 6, 12, and 24 months. MRI was performed preoperatively and at 24 months to assess tendon thickness. Results: All scores improved significantly. CMS increased from 16.3 ± 4.1 to 82.9 ± 5.8, VAS decreased from 7.8 ± 1.0 to 1.5 ± 0.8, and DASH improved from 70.3 ± 6.4 to 12.4 ± 4.5 (p < 0.05). Tendon thickness in the supraspinatus (T3) increased from 4.2 ± 0.9 mm to 6.8 ± 1.2 mm (p < 0.05). Retear rate was 7.55%, with no major complications. Conclusions: The bioinductive collagen implant showed notable results in improving tendon thickness, healing, and excellent clinical outcomes in RCR, without membrane-related complications. The study was designed as a prospective single-arm case series without a control group and that was the main limitation; The absence of adverse reactions in this cohort further supports the favorable safety profile of this implant in the present study population. Full article

(1) Achilles tendon rupture is one of the most severe lower-limb injuries, frequently occurring during movements involving maximal dorsiflexion with the knee at near-full extension. Preventive strategies are crucial, particularly for athletes engaged in high-risk sports such as basketball. (2) In this work, we examined the effect of a novel Achilles brace on Achilles tendon loading during concentric and eccentric mechanisms associated with tendon rupture. (3) Twenty-eight young basketball players performed tests under two conditions: with the adaptive brace and without it (control). Participants were divided into two groups (n = 14 in both). The first group assessed concentric Achilles loading by performing three plantar-flexor strength tests in three different joint configurations: maximal dorsiflexion with the knee flexed (FKF); injury mechanism position—full plantar flexion with the knee extended (FKE); and neutral ankle position with the knee extended (NKE). The number of maximal heel-raise repetitions performed before onset of fatigue was recorded. The second group assessed eccentric tendon loading by performing single-leg forced maximal-velocity dorsiflexion with the knee extended. In all tests, the time between maximal plantar flexion and maximal dorsiflexion, as well as the ankle range of motion, was analyzed using 2D video. Paired t-tests were used to compare braced and control conditions. In all tests, the ankle range of motion (ROM) did not differ significantly between brace and control conditions. Wearing the brace significantly improved plantar-flexor muscle strength only in the FKE test (31 ± 1.3 repetitions with brace vs. 21 ± 1.3 in control, p < 0.05). No significant differences were found for the FKF (27 ± 1.3 vs. 25 ± 1.3) or NKE (25 ± 1.3 vs. 24 ± 1.3) positions. During drop eccentric loading, wearing the brace resulted in a significantly slower transition time from plantar flexion to dorsiflexion (460 ± 60 ms with brace vs. 320 ± 30 ms in control, p < 0.001). (4) In brief, the novel Achilles brace was found to significantly reduces Achilles tendon load during both concentric and eccentric activities, but only in high-risk joint positions. These findings suggest that the brace provides mechanical protection, and may reduce the risk of Achilles tendon rupture, in athletes exposed to high tendon stress. Full article

Background/Objectives: Anterior segment optical coherence tomography (AS-OCT) and optical coherence tomography angiography (AS-OCTA) have enhanced the evaluation of the cornea, ocular surface, and ocular surface diseases (OSD), offering high-resolution structural and anterior segment vascular imaging. This review summarizes recent advances in these modalities and their clinical applications. Methods: A comprehensive literature search was conducted using PubMed, Web of Science, and Google Scholar with the terms OCT, OCTA, anterior segment, and ocular surface disease. Studies published in the past five years were included, emphasizing more recent developments such as ultra-high-resolution AS-OCT (UHR-AS-OCT) and swept-source AS-OCTA technologies. Results: UHR-AS-OCT provides non-invasive, sub-micron imaging of the cornea and the ocular surface, including tear film morphology and epithelial thickness to correlate with clinical tests such as tear break-up time, and fluorescein staining. Advances in AS-OCTA allow dye-free, depth-resolved imaging of corneal and conjunctival vasculature. These vascular biomarkers have shown promising utility in conditions such as limbal stem cell deficiency, chemical ocular injury, and ocular surface squamous neoplasia. Improvements in image acquisition, motion correction, and segmentation algorithms have enhanced accuracy and repeatability, supporting broader clinical translation. Conclusions: AS-OCT and AS-OCTA have become useful adjunctive imaging tools for the cornea and ocular surface evaluation. Their non-invasive, quantitative, and reproducible metrics may enable earlier diagnosis, objective staging, and longitudinal monitoring of OSD. Integration of OCT-based imaging with artificial intelligence and multimodal data, including tear proteomics and meibography, may optimize personalized treatment for ocular surface disorders. Full article

Dry eye disease (DED) is an ocular surface disorder characterized by tear film instability, inflammation, epithelial damage, and neurosensory abnormalities. Due to its multifactorial etiology and pathophysiology, conventional therapies that focus on lubrication and immunosuppression often fall short in addressing the neuropathic component of ocular pain experienced by a growing subset of patients. Recent developments in sensory neuroscience have highlighted the pivotal role of ion channels in mediating ocular surface homeostasis, pain signaling, and inflammation. This review examines the role of the following major ion channel families in the pathophysiology of DED and neuropathic ocular pain: transient receptor potential (TRP) channels, voltage-gated sodium (Nav) channels, and purinergic P2X receptors. The review details their anatomical distribution, molecular function, and responses to environmental stimuli such as heat, cold, osmolarity, and injury. Current treatments, such as artificial tears, anti-inflammatory drops, and systemic neuromodulators, are also reviewed in relation to their effects on ion channel modulation. Additionally, emerging therapies that directly target sensory transduction pathways are introduced. This review highlights the therapeutic potential of ion channel modulation in personalizing treatment for patients with ocular surface pain, particularly those with neuropathic features unresponsive to standard care. Full article

In this study, the potential for re-mixing and re-vulcanizing waste natural rubber glove (WNRG) material by using it as the primary matrix was investigated. Alternative types of vulcanization systems, namely, sulfur, phenolic resin, and peroxide, were employed. The results unequivocally demonstrated that residual vulcanizing agents contained in the WNRG were not sufficient to cause crosslinking reactions without re-mixing with vulcanizing agents. Among the various vulcanization approaches, sulfur produced the greatest properties, whereas phenolic resin gave moderate performance. The WNRG vulcanized with sulfur demonstrated the highest crosslink density, tear strength, tensile strength, hardness, and strain-induced crystallization ability among the tested alternatives. The tensile strength of WNRG vulcanized with sulfur was approximately 16.23 MPa, which was 31.7% and 51.1% greater than the WNRG vulcanizates made with phenolic resin and peroxide, respectively. Because of its highest crosslink density, the WNRG vulcanizate with sulfur also offers the greatest storage modulus among the tested cases. The results clearly suggest that the WNRG can potentially be re-compounded, re-vulcanized, and used as the primary matrix. WNRG could be used as a matrix at an industrial scale, to minimize the environmental issues and increase the added value from waste gloves. The findings provide practical guidance for recycling waste rubber gloves in industrial applications, which would be a more sustainable solution for solving the problems associated with WNRG. Full article

Duropathies represent a spectrum of disorders associated with spinal dural tears and cerebrospinal fluid (CSF) leaks. Diagnosis and treatment is often complicated by overlapping clinical manifestations. This review aims to synthesize current literature on duropathies, focusing on their clinical, neuroradiological, and pathophysiological features. A comprehensive literature review was conducted, analyzing various conditions classified as duropathies, including spontaneous intracranial hypotension (SIH), superficial siderosis (SS), spinal cord herniation, and, as added issue, arachnoid webs. The review emphasized the importance of imaging techniques such as MRI and CT myelography in diagnosing these conditions. Duropathies can arise from congenital anomalies, trauma, and degenerative changes, with SIH being characterized by orthostatic headaches and neurological deficits. Imaging typically reveals specific patterns, such as a widened dorsal subarachnoid space and ventral displacement of the spinal cord. Syringomyelia was frequently associated with arachnoid webs, and complications like SS and bibrachial amyotrophy were noted in patients with persistent ventral spinal CSF leaks. The unifying concept of duropathies is proposed, emphasizing the need for timely intervention to mitigate long-term neurological consequences. Enhanced diagnostic strategies are crucial for improving patient outcomes, and a multidisciplinary approach is recommended for the management of these complex disorders. Further research is warranted to clarify the pathophysiological mechanisms underlying duropathies and to establish standardized treatment protocols. Full article

A sucker rod pump is an artificial lift system widely used in oil wells to extract crude oil from deep underground. Due to the clearance between the barrel and the pump plunger, a phenomenon termed slippage occurs in which the annulus column of oil returns to the pump chamber due to the plunger motion and the pressure difference at the two ends of the plunger. Although it is important to maintain the clearance for lubrication between the plunger and the pump barrel in order to prevent excessive wear and tear along with galling, excessive clearance can also be a primary factor in the reduction of oil well production and must be managed. In this research, after briefly reviewing the Couette and Poiseuille flows within the annulus region, the relaxation time for the transients, and the eccentricity effects, we focus on the derivation of important system parameters such the effective mass, stiffness, and damping ratio based on the measurements of the sucker rod displacement and the pressures or loads. Analysis of experimental measurement data can provide better understanding of the sucker rod pump system parameters, helping to quantify and manage the so-called slippage issues. Full article

With the increasing emphasis and protection on forest resources worldwide, the development of non-wood plant fiber raw materials has become a key path to promote the green and sustainable development of China’s pulp and paper industry. In this study, Super-Arundo donax, a new non-wood fiber raw material, was systematically investigated for its applicability in the bleaching process. Firstly, by adjusting key bleaching technical variables such as alkali dosage, time, oxygen pressure and temperature, the oxygen delignification process of the Super-Arundo donax kraft pulp was optimized. The data revealed that under the experimental conditions of 3.0% alkali dosage, 60 min bleaching time, 100 °C bleaching temperature, 0.6 MPa oxygen pressure and 0.6% MgSO₄ dosage, the bleached pulp yield reached 91.58%, the brightness was 42.04% ISO, and its tensile index was 60.92 N·m/g, bursting index was 4.16 kPa·m²/g, and tear index was 5.45 mN·m²/g, respectively. To further enhance the bleaching effect, the study introduced the H₂O₂ enhanced oxygen delignification process. The alkali dosage, bleaching temperature and H₂O₂ dosage were selected as the process parameters, with the pulp yield and brightness as the response indicators. A central composite design was adopted to construct a response surface model, and the interaction effects among various factors were analyzed. The optimized optimal process conditions are as follows: pulp concentration 10%, alkali dosage 2.84%, bleaching temperature 105 °C, H₂O₂ dosage 4.85%, bleaching time 60 min, MgSO₄ dosage 0.6%. Under these conditions, the pulp yield was 89.76% and the brightness reached 53.85% ISO. Therefore, Super-Arundo donax possesses excellent pulp-making and papermaking properties, and is expected to serve as a high-quality non-wood fiber raw material to alleviate the pressure on traditional papermaking raw materials and contribute to the green, sustainable and low-carbon transformation of the pulp and paper industry. Full article

Rice straw as a growth substrate for soilless sod production not only avoids the damage to farmland soil deterioration but also solves the difficulty in disposing of a large amount of agricultural straw waste. This study was designed to explore the feasibility of using rice straw as a soilless sod production for seashore paspalum. The results showed that both fermented rice straw and raw rice straw significantly promoted the creeping growth and tillering of seashore paspalum, shortening the sod production period, when compared to the conventional soil sod. Rice straw sod significantly reduced sod weight to 50% and 52% of the soil sod, but increased sod strength to avoid tear damage in handling and transportation. Rice straw sod had 2 d longer shelf life than the soil sod, with slower decline of sod quality and maintained higher root and leaf emergence vigor during the sod storage. After sod installation, rice straw sod showed higher numbers of root and leaf emergence, and higher green leaves, stolons, new roots, aboveground and underground biomass, but lower thatch biomass, compared to the soil sod. Our results demonstrated that using rice straw as a growth substrate to produce soilless sod is feasible and significantly better than conventional soil sod production. Full article