Search Results (629)

Converting marine biowaste into nano-bioproducts for their application as bio-sourced, circular biostimulants to enhance crop productivity is a promising approach. This study evaluated chitosan–TPP nanoparticles (nanochitosan, ~38 nm) derived from blue crab (Callinectes sapidus) shells as a soil-applied biostimulant and conditioner for tomato (Solanum lycopersicum) grown in loam soil without mineral fertilizer. Our results showed that nanochitosan application as a soil supplement by drench improved the soil moisture content (39% vs. 22%), water-holding capacity (84% vs. 70%), total nitrogen (3.8 vs. 1.4 gm N kg⁻¹), and organic carbon content (48.4 vs. 34.1 gm C kg⁻¹) in nanochitosan-amended soil compared with the non-amended soil. This was accompanied by higher biomass, better root/shoot development and synthesis of phytohormones leading to increased shoot length, early flowering, and increased total soluble solids of fruits in nanochitosan-amended soil compared with control, suggesting that nanochitosan can act both as a beneficial soil conditioner and as a plant biostimulant. The results further show that nanochitosan-based formulations may be used not only as fertilizer-saving bio-inputs but also as bio-based nanochitosan plant biostimulants, which can partly substitute mineral fertilizer application for sustainable production of tomato. Moreover, generic fabrication of such nanochitosan from marine biowaste would support the circular-bioeconomy model to further improve sustainability of agroecosystems. Full article

Tea (Camellia sinensis) cultivation is a major global industry that faces sustainability challenges due to soil degradation and greenhouse gas (GHG) emissions from intensive management. Biochar—charcoal designed and used as a soil amendment—has emerged as a potential tool to improve soil health, enhance carbon sequestration, and mitigate GHG fluxes in agroecosystems. However, field-scale evidence of its effects on GHG dynamics in woody crops like tea remains limited, particularly regarding methane (CH₄). Here, we present, to our knowledge, the first field assessment of biochar impacts on CO₂, CH₄, and H₂O vapour fluxes in a subtropical tea agroforestry system with and without shade trees in northeastern Bangladesh. Using a closed dynamic chamber and real-time gas analysis, we found that biochar application (at 7.5 t·ha⁻¹) significantly enhanced average soil methane (CH₄) uptake by 84%, while soil respiration (CO₂ efflux) rose modestly (+18%) and water-vapour fluxes showed a marginal increase. Canopy conditions modulated these effects: biochar strongly enhanced CH₄ uptake under both shaded and open canopies, whereas biochar effects on water-vapour flux were detectable only when biochar was combined with a shade-tree canopy. Structural equation modelling suggests that CH₄ flux was primarily governed by biochar-induced changes in soil pH, moisture, nutrient status, and temperature, while CO₂ and H₂O fluxes were shaped by organic matter availability, temperature, and phosphorus dynamics. These findings demonstrate that biochar can promote CH₄ uptake and alter soil carbon–water interactions during the dry season in tea plantation systems and support operational biochar use in combination with shade-tree agroforestry. Full article

The red fox (Vulpes vulpes) is a widely distributed and highly adaptive small carnivore known by its generalist diet, which includes small mammals, invertebrates, and fruits. Despite its ecological relevance, how habitat heterogeneity affects its diet across the Mediterranean, a biodiversity hotspot shaped by long-term human disturbance, remains insufficiently synthesized. In this review, we synthesized and analyzed published studies that reported habitat-specific data on the red fox diet in the Mediterranean. Only 12 studies met the selection criteria, and no study directly compared two different habitats. The studied areas covered three dominant habitats: forests, scrublands (garrigue), and agroecosystems, and diet items were grouped in 7 categories: birds, carcasses, fruits, invertebrates, lagomorphs, small mammals, and reptiles. Overall diet composition varied significantly, with invertebrates and fruits being the most frequent diet items. In turn, lagomorphs and reptiles were the least frequent. In turn, diet composition varied little across habitats, indicating that diet variation follows specific local resource abundance regardless of habitat type. Despite the analytical limitations associated with the limited availability of habitat-explicit studies. The results highlight the pronounced dietary plasticity of the red fox and its capacity to integrate resource availability across heterogeneous Mediterranean landscape mosaics. This trophic adaptability and top predator role support various ecosystem functions such as controlling invertebrate and small mammal populations, dispersing seeds, and cycling nutrients, reinforcing the potential of the red fox as functional bioindicator in the Mediterranean. Therefore, sustainable land management, especially in agricultural areas, and restoration efforts for degraded areas should consider the beneficial roles of generalist carnivores like the red fox. Full article

Arbuscular mycorrhizal fungi (AMF) play a pivotal role in plant adaptation to arid ecosystems, yet their widespread agricultural use is constrained by the scarcity of high-quality, locally adapted inoculum. This study established a reliable monoxenic culture system for mass-producing an indigenous AMF isolate from the date palm (Phoenix dactylifera L.) rhizosphere in the Figuig oasis, southeastern Morocco. The isolate was identified as Rhizophagus irregularis based on spore morphology and Large Subunit ribosomal DNA (LSU rDNA) phylogeny. Two propagule types, surface-sterilized spores and mycorrhizal root fragments of Plantago lanceolata L., were compared for initiation of in vitro cultures on Ri T-DNA-transformed carrot (Daucus carota L.) hairy roots. By week 16, cultures initiated from mycorrhizal root fragments produced 1414 ± 65 spores per plate and showed significantly higher performance than spore-derived cultures in terms of propagule viability, root colonization, and hairy root growth. Propagule viability reached 84% and 68%, root colonization frequencies were 95% and 72%, and hairy root lengths averaged 81 and 63 cm in root fragment- and spore-derived cultures, respectively (p < 0.01). In a subsequent whole-plant assay using P. lanceolata, in vitro-produced spores induced markedly higher mycorrhizal colonization frequency (91.0 ± 1.6% compared with 74.8 ± 1.9%) and intensity (70.0 ± 1.6% compared with 55.0 ± 1.6%) than spores obtained from conventional trap cultures (p < 0.001). These results demonstrate that monoxenic root-organ culture using root fragments is a robust, reproducible method for generating abundant, contaminant-free, and functionally superior inoculum of native R. irregularis. This advance provides a solid platform for developing tailored bio-inoculants to enhance crop resilience and sustainability in arid and semi-arid agroecosystems. Full article

The Arid Region of Northwest China (ARNC) functions as a critical ecological barrier for the Eurasian hinterland. To clarify the non-linear drivers of eco-environmental dynamics, a long-term (2000–2024) Remote Sensing Ecological Index (RSEI) time series was constructed and analyzed using an interpretable machine learning framework (XGBoost-SHAP). The analysis reveals pronounced spatial asymmetry in ecological evolution: improvements are concentrated in localized, human-managed areas, while degradation occurs as a diffuse process driven by geomorphological inertia. The ARNC exhibits low-level stability (mean RSEI 0.25–0.30) and marked unbalanced dynamics, with significant degradation (19.9%) affecting more than twice the area of improvement (6.5%). Attribution analysis identifies divergent driving mechanisms: ecological improvement (R² = 0.559) is primarily anthropogenic (58.3%), whereas degradation (R² = 0.692) is mainly governed by natural constraints (58.4%), particularly structural topographic factors, where intrinsic landscape vulnerability is exacerbated by human activities. SHAP analysis corroborates a “Greenness-Quality Paradox” in stable agroecosystems, where high vegetation cover coincides with reduced evaporative cooling and secondary salinization from irrigation, resulting in declining Eco-Environmental Quality (EEQ). A zero-threshold effect for grazing intensity is also identified, indicating that any increase beyond the baseline immediately initiates ecological decline. In response, a Resist-Accept-Direct (RAD) framework is proposed: direct salt-water balance regulation in oases, resist hydrological cutoff in ecotones, and accept natural dynamics in the desert matrix. These findings provide a scientific basis for reconciling artificial greening initiatives with hydrological sustainability in water-limited regions. Full article

Tillage practices critically influence soil’s physical properties, which are fundamental to sustainable agriculture in temperate climates. This review evaluates how conventional tillage (CvT; e.g., moldboard and chisel plowing), reduced tillage (RT), and conservation tillage (CT), particularly no-tillage (NT), affect six key indicators: bulk density (BD), saturated hydraulic conductivity (Ks), wet aggregate stability (WAS), penetration resistance (PR), available water capacity (AWC), and soil organic carbon (SOC). Special emphasis is placed on differentiating topsoil and subsoil responses to inform climate-resilient land management. A total of 70 peer-reviewed studies published between 1991 and 2025 were analyzed. Data were extracted for BD, Ks, WAS, PR, AWC, and SOC across tillage systems. Depths were standardized into topsoil (0–10 cm) and composite (>10 cm) categories. Descriptive statistics were used to synthesize cross-study trends. NT showed lower mean BD in the topsoil (1.32 ± 0.08 Mg/m³) compared with moldboard plow (1.33 ± 0.09) and chisel tillage (1.39 ± 0.12); however, the effects of tillage on BD were not statistically significant, while BD was higher at composite depths under NT (1.56 ± 0.09 Mg/m³), indicating subsoil compaction. Ks improved under NT, reaching 4.2 mm/h with residue retention. WAS rose by 33.4%, and SOC increased by 25% under CT systems. PR tended to be elevated in deeper layers under NT. Overall, CT, particularly NT, improves surface soil’s physical health and SOC accumulation in temperate agroecosystems; however, persistent subsoil compaction highlights the need for depth-targeted management strategies, such as controlled traffic, periodic subsoil alleviation, or deep-rooted cover crops, to sustain long-term soil functionality and climate-resilient production systems. Full article

Sorghum (Sorghum bicolor L. Moench) is a major cereal crop cultivated in semi-arid regions, but its yield is often constrained by soilborne fungal pathogens that affect plant growth and grain quality. This study explored how Trichoderma-based bioinoculants restructure the structure and functional composition of fungal communities in distinct sorghum compartments (soil, root, seed, and stem) using ITS amplicon sequencing. Two cultivars, Kalatur and Foehn, were evaluated under control and inoculated conditions. Alpha diversity indices revealed that inoculation reduced overall fungal richness and evenness, particularly in seed and stem tissues, while selectively enhancing beneficial taxa. Beta diversity analyses (PERMANOVA, p < 0.01) confirmed significant treatment-driven shifts in community composition. LEfSe analysis identified Trichoderma and Mortierella as biomarkers of inoculated samples, whereas Fusarium, Alternaria, and Penicillium predominated in controls. The enrichment of saprotrophic and symbiotrophic taxa in treated samples, coupled with the decline of pathogenic genera, indicates a transition toward functionally beneficial microbial assemblages. These results demonstrate that Trichoderma bioinoculants not only suppress fungal pathogens but also promote the establishment of beneficial ecological groups contributing to plant and soil health. The present work provides insight into the mechanisms through which microbial inoculants modulate host-associated fungal communities, supporting their use as sustainable tools for crop protection and microbiome management in sorghum-based agroecosystems. Full article

Root exudates are key mediators of plant–soil–microbe interactions, shaping rhizosphere dynamics and influencing agroecosystem resilience. Comprising diverse primary and secondary metabolites, these compounds are actively secreted through specific transport pathways and are modulated by intrinsic plant traits and environmental conditions. Root exudates serve as chemical signals that recruit and structure microbial communities, facilitating nutrient mobilization, microbial feedbacks, and the regulation of plant growth and stress responses. By modulating soil chemical, physical, and biological properties, exudates contribute to carbon cycling, soil health, and the maintenance of ecosystem services. Moreover, they play multifunctional roles in enhancing plant tolerance to abiotic and biotic stresses, while also mediating interactions with neighboring plants. This review provides a holistic perspective on root exudation, encompassing their mechanisms and drivers, roles in rhizosphere ecology and plant stress adaptation, and methodological advances, while highlighting opportunities to harness these processes for resilient, productive, and sustainable agroecosystems. Full article

Carbon (C) is crucial for nutrient cycling and the assembly of microbial populations in the soil. However, it is still unclear how the C-source utilization characteristics of microbes in distinct types of soils respond to changes in soil phosphorus (P) activity. This study investigated how the addition of different C sources with different decomposition rates (glucose, hemicellulose, and lignin) affects P transformation in two distinct agricultural soils (i.e., Mollisols and Fluvo-aquic soil). Results revealed that the short-term glucose addition to soil induced rapid acidification and microbial biomass accumulation, thereby significantly increasing labile P (NaHCO₃-P_i, NaOH-P_o) content in Fluvo-aquic soil. Lignin amendment promoted gradual HCl-P release in Mollisols, reflecting differential microbial utilization strategies. Glucose stimulated phosphatase activity (2.5–3.0× control) and phoD gene abundance (4.8×) in Fluvo-aquic soil in the early stage, favoring the growth of Pseudomonas and Burkholderia, whereas lignin sustained the mineralization of fungal-associated P in Mollisols (1.8–2.3× phosphatase activity) by enhancing the abundance of Streptomyces and Bradyrhizobium. Soil type dictated P mobilization efficiency. The Fluvo-aquic soil exhibited rapid but transient P release via bacterial dominance, while Mollisols retained slower yet persistent P availability through specialized microbial consortia. Notably, glucose enhanced organic P mineralization by stimulating C decomposition by microbes, particularly in C-rich Mollisols. Lignin increased P availability in Mollisols via Fe/Al-P desorption. However, in Fluvo-aquic soil, lignin reduced the availability of P through microbial immobilization. These findings highlight that C source degradability and soil properties interactively govern microbial-mediated P cycling in soil. Therefore, organic amendments in contrasting agroecosystems need to be optimized. Full article

Wheat (Triticum aestivum L.) sustains global caloric intake, but its productivity in Andean highlands is constrained by soil fertility and input reliance. This study represents one of the first field-based evaluations of biofertilizers under high-altitude, rainfed Andean conditions, addressing a major knowledge gap in low-input mountain agroecosystems. This study evaluated three seed-applied biofertilizers—Azospirillum brasilense, Trichoderma viride (Trichomax), and an anchovy (Engraulis ringens) based liquid biofertilizer, compared with an untreated control and a soil-test mineral fertilization benchmark in rainfed wheat (Triticum aestivum L.) cv. INIA 405 in the central Andes of Peru. A 5 × 5 Latin square design (25 plots) was established under farmer-realistic conditions. At physiological maturity (Zadoks 9.5), plant height, spike length, grains per spike, thousand-grain weight, test weight, root dry mass, and grain yield were recorded. Mineral fertilization achieved the highest yield (1.20 ± 0.79 t ha⁻¹), nearly doubling the control (0.60 ± 0.47 t ha⁻¹). Notably, A. brasilense delivered an intermediate yield of 0.90 ± 0.64 t ha⁻¹, representing a 50% increase over the control—accompanied by a marked rise in root dry mass. T. viride and the anchovy-based input yielded 0.85 ± 0.59 and 0.81 ± 0.59 t ha⁻¹, respectively. Grain physical quality remained stable across treatments (thousand-grain weight ≈ 42 g; test weight 68–75 kg hL⁻¹). Trait responses were complementary: root dry mass increased with mineral fertilization and A. brasilense, whereas spike length increased with mineral fertilization and the anchovy-based input. Overall, the evidence supports biofertilizers, particularly A. brasilense, as effective complements that enable partial fertilizer substitution within integrated nutrient-management strategies for sustainable wheat production in Andean rainfed systems. Full article

Quinoa (Chenopodium quinoa Willd.) is a strategic crop for climate-smart agriculture in the Andes, yet yield gains are constrained by soil degradation and low-input systems. We tested whether synergistic bioinoculation with a plant growth-promoting rhizobacterium (Azospirillum brasilense) and an arbuscular mycorrhizal fungus (Glomus iranicum var. tenuihypharum) enhances root function and grain productivity under field conditions. A split-plot RCBD was conducted in Ayacucho, Peru (2735 m a.s.l.) using four cultivars, Blanca de Junín (BJ), INIA 441 Señor del Huerto (SH), INIA 415 Pasankalla (RP) and INIA 420 Negra Collana (NC) and four treatments: uninoculated control, Azospirillum, Glomus and co-inoculation. Vegetative, root and yield traits were quantified; ANOVA, Tukey/Dunnett contrasts, correlations and PCA were applied. Co-inoculation consistently outperformed single inoculants, increasing root diameter, length, branching, dry weight and volume dry weight, while also enlarging panicle dimensions and raising grain weight per panicle and thousand-seed weight. Grain yield reached 4.94 ± 0.59 t ha⁻¹ under co-inoculation, almost triple that of the control (1.71 ± 0.28 t ha⁻¹) and about 1.5 times higher than single inoculations. Genotypic effects were pronounced; BJ and SH combined superior root biomass with higher yield, RP maximized grain size and hectoliter weight, whereas NC responded weakly. Significant genotype × treatment interactions indicated cultivar-dependent microbiome benefits. Correlation and PCA linked root biomass and stem/panicle architecture to yield formation, positioning co-inoculation along trait vectors associated with belowground vigor and productivity. These results demonstrate a robust microbial synergy that translates root gains into yield, supporting co-inoculation as a scalable, low-input strategy for sustainable intensification of quinoa in highland agroecosystems. Full article

The widespread cultivation of transgenic Bt cotton has elevated Apolygus lucorum (Meyer-Dür) to a major pest in cotton agroecosystems. Its rapidly developing resistance to insecticides poses a serious challenge to sustainable agriculture. In this study, we assessed the susceptibility of a field-collected population from Anyang, Henan Province, in relation to a laboratory-susceptible strain, to elucidate the present status and molecular basis of resistance to beta-cypermethrin. First, the toxicity of beta-cypermethrin to A. lucorum was assessed through a diet-incorporation method. Subsequently, the enzymatic activities of carboxylesterase (CarE) and glutathione S-transferase (GST) were measured, and the expression levels of CarE1 and GST1 were quantified by quantitative real-time PCR (qRT-PCR). Finally, the function of candidate genes was confirmed using RNA interference (RNAi) technology. The bioassays results indicated that the median lethal concentration (LC₅₀) for the laboratory and Anyang field strain were 343.34 mg/L and 700.45 mg/L, respectively. Following 48 h of exposure to the LC₃₀ of the susceptible strain, the mortality rate of the field population (20.00%) was significantly lower than that of the laboratory population (33.33%), suggesting an increase in resistance. The field population of A. lucorum exhibited significantly higher activities of CarE (1.61-fold) and GST (1.71-fold) compared to the laboratory strain, accompanied by 3.63- and 4.23-fold overexpression of the corresponding genes CarE1 and GST1. Spatiotemporal expression profiling revealed that CarE1 expression was highest in 4th–5th instar nymphs and adults, with predominant localization in the midgut, while GST1 expression peaked in 4th–5th instar nymphs and was abundant in the midgut and fat body. RNAi-mediated knockdown of CarE1 and GST1 significantly enhanced susceptibility to beta-cypermethrin in field populations, resulting in elevated mortality 48 h post-treatment compared to controls. In conclusion, the field population of A. lucorum has developed considerable resistance to beta-cypermethrin, strongly correlated with overexpression of CarE1 and GST1. These results deepen our understanding of metabolic resistance mechanisms and offer valuable insights for developing targeted pest control strategies. Full article

Agricultural systems in Mediterranean-type climates are increasingly threatened by drought, salinity, extreme temperatures, heavy metal contamination, and pathogen pressure, all of which undermine crop productivity and agroecosystem stability. In this context, arbuscular mycorrhizal fungi (AMF), natural symbionts of most terrestrial plants, emerge as key biological agents capable of enhancing crop resilience. Following PRISMA guidelines, this systematic review synthesizes current knowledge on the role of AMF in mitigating abiotic and biotic stresses, highlighting their potential as a central component of sustainable Mediterranean agriculture. The available evidence demonstrates that AMF symbiosis significantly increases plant tolerance to multiple stressors across major crop families, including Poaceae, Fabaceae, Solanaceae, and Asteraceae. Under abiotic constraints, AMF improve water and nutrient uptake via extensive hyphal networks, modulate ion homeostasis under salinity, enhance tolerance to thermal extremes, and reduce heavy metal toxicity by immobilizing contaminants. Regarding biotic stresses, AMF induce systemic resistance to pathogens, stimulate secondary metabolite production that deters herbivores, and suppress parasitic nematode populations. Moreover, co-inoculation with other biostimulants, such as plant growth-promoting rhizobacteria, shows synergistic benefits, further improving crop productivity and resource-use efficiency. Overall, AMF represent an effective and multifunctional nature-based tool for improving the sustainability of Mediterranean agroecosystems. However, further research is required to evaluate AMF performance under simultaneous multiple stress factors, thereby reflecting real-world conditions and enabling a more integrated understanding of their agronomic potential. Full article

The world faces increasing food, environmental, and human security issues, primarily attributed to an overburdened agricultural sector struggling to keep pace with rising population and demand for food, energy, and fiber. Advances in food production and agriculture, especially with monoculture farming, have continued to meet these demands but at a high price regarding resource depletion and environmental devastation. This is especially severe in developing world areas with rural populations with thin resource margins. Regenerative agriculture has emerged as a solution to provide shielding for food production, ensure environmental protection, and promote social equity while addressing many of these issues. Regenerative agriculture food production aims to restore soils, forests, waterways, and the atmosphere and operate with lower offsite negative environmental and social impacts. This review discusses the fundamental principles and practices of sustainable plant protection for regenerative farming. It focuses on the role of biological and ecological processes, reduces non-renewable inputs, and aims to incorporate traditional ecological knowledge into pest control practices. It offers essential transition strategies, including critical changes from conventional integrated pest management (IPM) to agro-ecological crop protection, focusing on systemic approaches to design agroecosystems. It also reaffirms the importance of a vast diversity of pest control methods that are culturally, mechanistically, physically, and biologically appropriate for regenerative farming practices. Ultimately, the aim is to encourage ecological, economic, and social sustainability for the future of more resilient and controlled agricultural practices. Full article

Agroecology is increasingly shaped by the convergence of traditional knowledge, farmers’ lived experiences, and scientific research, fostering a plural dialog that embraces the ecological and socio-political complexity of agricultural systems. Within this framework, soil biodiversity is essential for maintaining ecosystem functions, with soil microbiology, and particularly arbuscular mycorrhizal fungi (AMF), playing a pivotal role in enhancing soil fertility, plant health, and agroecosystem resilience. This review explores the synergy between agroecological practices and AMF by examining their ecological, economic, epistemic, and territorial contributions to sustainable agriculture. Drawing on recent scientific findings and Latin American case studies, it highlights how practices such as reduced tillage, crop diversification, and organic matter inputs foster diverse and functional AMF communities and differentially affect their composition and ecological roles. Beyond their biological efficacy, AMF are framed as relational and socio-ecological agents—integral to networks that connect soil regeneration, food quality, local autonomy, and multi-species care. By bridging ecological science with political ecology and justice in science-based knowledge, this review offers a transdisciplinary lens on AMF and proposes pathways for agroecological transitions rooted in biodiversity, cognitive justice, and territorial sustainability. Full article