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Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles
- Pearl Akamine,
- José A. González-Feliciano,
- Ruth Almodóvar,
- Gloriner Morell,
- Javier Rivera,
- Coral M. Capó-Vélez,
- Manuel Delgado-Vélez,
- Luis Prieto-Costas,
- Bismark Madera and
- Daniel Eichinger
- + 6 authors
We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate. First in shake flasks, then in bioreactors, we optimiz...