Search Results (148)

Constructed wetlands (CWs) have emerged as effective nature-based solutions (NbS) for the treatment of industrial dairy wastewater (DWW), which is characterized by high organic loads, elevated nutrient concentrations, and pronounced operational variability. Despite increasing implementation, quantitative engineering evidence supporting design optimization and scalability remains fragmented. Herein, we present a semi-quantitative synthesis of CW performance for DWW treatment, explicitly linking hydraulic and operational parameters with pollutant removal efficiencies. A systematic review of 38 peer-reviewed studies published between 1995 and 2025 was conducted in accordance with PRISMA 2020 guidelines. Treatment performance was normalized and evaluated as a function of hydraulic retention time (HRT), organic loading rate (OLR), system configuration, and climatic context. The results demonstrate that hybrid CWs combining vertical and horizontal subsurface flow most frequently achieved COD and BOD₅ removal efficiencies exceeding 90% when operated within an observed operating envelope, typically including HRT ranges of 4–8 h (VSSF; n = 4) and 3–7 days (HSSF; n = 14), and OLR values below 30 g COD m⁻² d⁻¹ (n = 7, among studies reporting OLR). Operation outside this operating envelope was generally associated with reduced treatment stability and an increased likelihood of operational constraints (e.g., clogging). Substrate porosity, vegetation diversity, and climate further modulated long-term performance and system resilience. Based on the consolidated evidence, this review suggests transferable operational design envelopes and configuration-specific implementation pathways that translate empirical findings into practical engineering guidance, supporting the scalable adoption of CWs as low-energy NbS for decentralized and sustainable DWW management. Full article

Mitral annular disjunction (MAD) is associated with an increased risk of ventricular arrhythmias and sudden cardiac death, yet its genetic background remains poorly defined. We report the case of a 50-year-old man with MAD who survived cardiac arrest and carries three variants of unknown significance (VUS) in genes involved in cardiomyopathy pathogenesis. To explore the genetic basis of non-syndromic MAD, we performed a systematic review of the literature, identifying five case reports and one retrospective cohort study. The case reports described patients with MAD harboring four pathogenic variants and ten VUS. Two pathogenic variants were linked to cardiomyopathies, involving proteins of the nuclear envelope and cytoskeleton, while two were associated with channelopathies. The retrospective cohort study identified a recurrent variant in a gene involved in intercellular adhesion segregating within a family affected by MAD. Overall, available evidence suggests that genetic factors may hypothetically modulate susceptibility to MAD, not only in connective tissue disorders but also in isolated mitral valve disease. Variants associated with arrhythmogenic cardiomyopathies and channelopathies appear to cluster in families with non-syndromic MAD and arrhythmic phenotypes, suggesting a role in the arrhythmic substrate. However, in absence of definitive functional, segregation, or longitudinal data, the contribution of genetic variants to MAD should be interpreted with caution. Further genomic studies are needed to clarify their genetic contribution and prognostic implications. Full article

Antimicrobial resistance (AMR) was associated with 4.95 million deaths in 2019 and may cause 10 million deaths annually by 2050. We synthesize evidence on how the black soldier fly (Hermetia illucens) has evolved an expanded antimicrobial peptide (AMP) repertoire, which structural features drive family-specific activity, what mechanisms are directly demonstrated in H. illucens, and how AI contributes. PubMed, Web of Science, and Scopus (plus targeted Google Scholar) were searched from inception to 1 February 2026; studies were included when they reported BSF peptide identities, expression/proteomics, evolutionary analyses, quantitative activity, mechanistic assays, or BSF-focused computation, and claims were tiered as predicted, expression-supported, or experimentally supported. The literature supports 50–80 BSF AMP genes, plausibly shaped by gene duplication and balancing/diversifying selection in microbe-rich substrates, with marked induction plasticity across tissues, development, diet, and challenge. SAR is family-dependent: defensin-like peptides rely on disulfide-stabilized CSαβ folds and cationic surface topology; cecropin-like peptides on amphipathic α-helices with selectivity trade-offs; attacin-like peptides on β-architecture where charge-based heuristics are weak; and diptericin/proline-rich peptides remain largely inference-driven in BSF. Mechanistic evidence is strongest for membrane/envelope-centered killing by DLP4 and pore-associated envelope disruption by a recombinant attacin-like peptide, whereas pore geometry, oligomerization, intracellular targets, and broad “resistance-proof” claims remain unresolved. Key gaps include assay heterogeneity, salt/serum stability, selectivity/toxicity, resistance-risk testing, and limited in vivo validation, which must be addressed for credible AMR-relevant translation. Full article

Implant science has traditionally treated “biocompatibility” as the master criterion of success, focusing on cytotoxicity, corrosion, immune response, infection control, and the chemical stability of materials in vivo. However, many clinically “biocompatible” devices still fail at the point where the body actually meets the device: the mechanical interface. The interface is not a passive boundary. It is a living, adapting, mechanosensitive microenvironment in which cells integrate stiffness, micromotion, surface roughness, fluid shear, and wear debris with biochemical signals to decide whether to incorporate an implant, wall it off, resorb adjacent tissue, or trigger chronic inflammation. In load-bearing orthopaedics, stiffness mismatch produces stress shielding and maladaptive remodelling; excessive micromotion drives fibrous encapsulation rather than osseointegration; abrasive wear creates particulates that sustain macrophage activation and osteolysis; and design choices that are mechanically adequate in bench tests can still fail in vivo when the implant–tissue system evolves. In soft-tissue implantation, substrate stiffness can be a primary driver of the foreign body response and fibrotic capsule formation through mechanosensitive pathways, such as TRPV4-mediated macrophage–fibroblast signalling. Mechanical compatibility is not a replacement for classical biocompatibility; rather, it should be treated as a co-equal, first-class design requirement in mechanosensitive organisms. Chemically biocompatible materials can still fail through stiffness mismatch, micromotion, fretting and wear debris generation, and mechanobiology-driven fibrosis or osteolysis. We therefore propose a process view of implant success: tissue mechanics should be measured in clinically relevant states, transformed into constitutive models and interface performance envelopes, translated into explicit mechanical-compatibility specifications, and then realised through manufacturing process windows that can reliably reproduce targeted architectures and surface states. Additive manufacturing and microstructural engineering enable the tuning of modulus, the formation of porosity gradients, and the generation of patient-specific compliance fields, but these advances only improve outcomes when coupled to metrology, statistical process control, and validation loops that close the gap between intended and realised interface mechanics through clinical surveillance. Full article

This paper presents a dual four-port circularly polarized (CP) MIMO antenna based on substrate integrated waveguide (SIW) technology for sub-6 GHz applications. The design consists of two identical four-port SIW-based CP-MIMO antennas arranged in a mirror-symmetric configuration with an air gap of 15 mm. Each antenna employs four symmetrically arranged cross-shaped SIW patches excited by coaxial probes. Bidirectional radiation is achieved by applying a 180° phase difference between corresponding ports of the mirror symmetric configuration, referred to as the Backward-Radiating Unit (BRU) and the Forward-Radiating Unit (FRU). The bidirectional radiation mechanism is supported by array-factor-based theoretical modelling, which explains the constructive and destructive interference under phase-controlled excitation. To ensure high isolation and stable polarization performance, the antenna design incorporates defected ground structures, inter-element decoupling strips, and vertical metallic vias. Simulations indicate an operating band from 5.1 to 5.4 GHz. Measurements show a −10 dB bandwidth from 5.25 to 5.55 GHz, with the frequency shift attributed to fabrication tolerances and measurement uncertainties. The antenna achieves inter-port isolation better than −15 dB. A 3 dB axial-ratio bandwidth is maintained across the operating band. Measured axial-ratio values remain below 3 dB from 5.25 to 5.55 GHz, while simulations predict a corresponding range from 5.1 to 5.4 GHz. The proposed configuration achieves a peak gain exceeding 4 dBi and maintains an envelope correlation coefficient below 0.05. These results confirm its suitability for CP-MIMO systems with controlled spatial coverage. With a physical size of 0.733λ₀ × 0.733λ₀ per array, the proposed antenna is well-suited for vehicular and space-constrained wireless systems requiring bidirectional CP-MIMO coverage. Full article

Ceramides are central to stratum corneum barrier organization and hydration. Beyond topical replenishment, ceramide-stimulating strategies increasingly aim to enhance endogenous ceramide biosynthesis, processing, and homeostatic remodeling in coordination with keratinocyte differentiation. In this review, we summarize the three major metabolic routes that shape epidermal ceramide output—de novo synthesis, salvage, and sphingomyelin hydrolysis—and organize representative bioactive ingredients by their primary molecular targets rather than by origin. Specifically, we map ingredients to tractable regulatory nodes, including transcriptional “liposensors” (PPAR/LXR), the induction of biosynthetic/elongation and processing enzymes (e.g., SPT, CerS3, ELOVL4), the provision of structural substrates and precursors (e.g., linoleate-rich lipids and glycosylceramides), salvage-pathway sphingoid bases that can reshape ceramide subclass output, and metabolic sensing/stress-response pathways centered on AMPK–mTOR–SIRT1/autophagy. Across these mechanisms, agents spanning botanical and fermented extracts, vitamins, sphingoid intermediates, lipid precursors, and pathway modulators (including autophagy-focused probes) have been reported to increase ceramide abundance and, in some contexts, favor barrier-relevant ultra-long-chain species and ω-O-acylceramides that support lamellar organization and the corneocyte lipid envelope. Translational and clinical studies in dry, sensitive, and aged skin generally associate such interventions with improved barrier function and reduced dryness. Aligning ingredient selection with defined biosynthetic and processing checkpoints—and verifying outcomes with lipidomics alongside clinical endpoints—may accelerate the development of evidence-based, ceramide-stimulating cosmetics. Full article

This paper presents a compact four-port multiple-input multiple-output (MIMO) antenna operating in the Ku-band around 13 GHz, targeting early-phase 6G upper-midband front-end applications. The proposed antenna employs orthogonally arranged short-ended square patch elements combined with a modified defected ground structure (DGS) to achieve high port isolation and compact footprint. A prototype fabricated on Rogers RO4350 substrate demonstrates good agreement between simulated and measured results. The antenna achieves $|S_{11}|$ < −10 dB over 12.9–13.1 GHz band, inter-port isolation exceeding 25 dB, and an envelope correlation coefficient (ECC) below $0.01$. The measured realized gain reaches 7.02 dBi with a radiation efficiency above 80%. Compared with recent Ku-band MIMO antennas, the proposed design provides a 45% size reduction while maintaining high isolation at a close element spacing of 0.25${\lambda }_0$. The proposed antenna intentionally adopts a narrowband operating characteristic, reflecting a design trade-off that prioritizes compact size, high isolation, and low spatial correlation over wideband performance. These features make the antenna well suited for early-stage 6G-oriented front-end modules, fixed wireless access, backhaul links, and short-range sensing systems operating in the upper-midband frequency range. Full article

Human transmembrane proteins Serinc3 and Serinc5 are antiviral restriction factors that inhibit HIV-1 infectivity. In the absence of viral antagonism, Serinc3 and Serinc5 incorporate into the envelopes of nascent virions and inhibit the fusion of virions to the target cells. The HIV-1 virus counteracts the restriction of Serinc3 by downregulating it from the cell surface and thus excluding it from budding virions. This is orchestrated by the viral accessory protein Nef and involves hijacking of the clathrin adaptor protein complex 2 (AP2)-dependent endocytosis. The mechanistic details of Nef-mediated Serinc3 downregulation, however, have been enigmatic. In this work, we investigated and revealed the molecular determinants of Serinc3 modulation by Nef. Our results show that Nef recruits Serinc3 by binding to its N-terminal cytosolic tail. Furthermore, Nef residues important for Serinc3-binding in vitro, and for the exclusion of Serinc3 from virions, overlap with those required for Nef-mediated CD4 downregulation, suggesting great mechanistic similarities between the two functions of Nef. In addition to shedding light on the mechanism of Serinc3 antagonism, our work also highlights the conserved substrate-binding pocket of Nef as a molecular hotspot for inhibitor development and antiretroviral drug discovery. Full article

Heat is widely used to decontaminate livestock environments, yet performance varies with virus, surface, moisture, and organic load. We evaluated the effects of temperature (50, 60, 70 °C) and exposure time on the viability of 10 veterinary-relevant viruses (or surrogates) placed on four nonporous surfaces (plastic, rubber, aluminum, stainless steel) under dry or wet conditions, and in organic matrices (blood, wheat straw, complete feed). Infectivity was quantified by TCID₅₀ using independent duplicate experiments with duplicate titrations. Moist heat consistently outperformed dry heat: at 60–70 °C, all enveloped viruses, and most non-enveloped viruses were inactivated on surfaces within 5 min, while porcine parvovirus (PPV) remained the outlier, requiring ≥60 min. In contrast, dry heat allowed several viruses to persist for 24 h at 70 °C, underscoring that temperature alone is an unreliable predictor of rapid decontamination in the absence of humidity. Organic matrices modulated outcomes in a substrate- and virus-dependent manner, with some combinations accelerating inactivation and others prolonging survival to ≥180 min at ≥60 °C. These findings support matrix-aware, heat-assisted protocols for facilities and transport (e.g., 70 °C for ≥10 min under high humidity for most enveloped viruses), while recognizing exceptions such as PPV. The data provide actionable parameters to optimize thermo-assisted decontamination in veterinary systems. Full article

Inorganic phase change materials (PCMs) can be employed in passive thermal regulation systems as building envelopes to decrease energy consumption. Nonetheless, they present a manifold of issues, such as leakage, incongruent melting, crystallization, and supercooling, which limit their performance and durability. A widely explored approach to address these shortcomings is the development of eutectic salts and their stabilization through techniques such as the use of porous substrates and encapsulation, in addition to combining them with the incorporation of carbon derivatives as fillers and nucleating agents to enhance thermal performance and durability during charge and discharge cycles. In this study, a critical review is developed via analysis and discussions of different methods for incorporating inorganic PCMs. The focus is mainly on eutectic salts and the challenges associated with their application, the generation of new eutectic salts, stabilization methods, and use cases where the incorporation of fillers, the use of porous substrates, and the implementation of nucleating agents have contributed to improving thermal performance, reducing the degree of supercooling, and minimizing PCM leakage during phase transitions. Full article

The efficient removal of failed yttria-stabilized zirconia (YSZ) thermal barrier coatings from GH4169 superalloy substrates is crucial for aero-engine maintenance. This study investigates the application of plasma electrolytic technology for YSZ coating removal, systematically examining the effects of key process parameters. Through a three-factor, five-level orthogonal experimental design, the influence of working voltage, solution temperature, and processing time on coating removal effectiveness was analyzed using range analysis. The results demonstrated that solution temperature exerted the most significant effect on coating removal rate, followed by working voltage, with processing time showing the least influence. The optimal parameter combination was determined as 265 V working voltage, 50 °C solution temperature, and 120 s processing time, achieving a maximum coating removal rate of 92.36%. The underlying mechanisms were elucidated through detailed characterization: at 250 V, micro-arc discharge enabled effective coating removal through combined physical bombardment and electrochemical dissolution, while at 300 V, arc discharge caused substrate damage with crater formation. Solution temperature critically affected process stability through its regulation of vapor-gaseous envelope characteristics and current behavior. Verification experiments confirmed that the optimized parameters achieved complete coating removal without substrate damage, preserving surface integrity for subsequent recoating processes. This research provides both theoretical foundation and practical parameters for plasma electrolytic removal of YSZ coatings on hot-section components. Full article

The widespread use of transparent building envelope structures satisfies people’s needs for architectural esthetics and daylighting. However, they also feature notable drawbacks such as high energy consumption, poor thermal insulation performance of traditional glass curtain walls, significant solar heat gain in summer and heat loss in winter, which lead to “cold in winter and hot in summer” indoors, reliance on high-power air conditioning, and energy consumption far exceeding that of opaque walls. Even when coated or insulated glazing is adopted, improper design can still fail to effectively reduce the overall heat transfer coefficient, placing higher demands on the daylighting performance and solar radiation control of transparent envelopes in existing buildings. Through experiments and numerical simulations, this study systematically analyzes the performance of different types of glass used in transparent building envelope structures and their impacts on building energy consumption. Based on the climatic characteristics of central-southern Anhui, measured data were compared between a Low E-glass sunroom and a conventional tempered glass sunroom. The results show that the solar radiation transmittance of the Low-e glass is only 45.31% of that of ordinary glass, the peak indoor temperature is reduced by 6–7 °C, and nighttime temperature fluctuations are smaller, verifying its excellent thermal insulation performance and thermal stability. To further investigate, the Ecotect software 2011 was used to simulate the daylighting performance of 12 types of glazing and the radiation transmittance under 19 conditions. The results indicate: triple-glazed vacuum composite silver-coated glass exhibits excellent shading performance suitable for summer; single-silver-coated glass has the best daylighting performance, and Triple-Silver coatings combined with high-transmission substrates can improve the daylight factor by 10.55%; argon-filled insulated glazing reduces radiation by 6.5% compared with ordinary IGUs, making it more suitable for the climate of central-southern Anhui. The study shows that optimization of transparent envelopes must be predicated on regional climate, combining experimentally validated glazing thermal parameters with simulation-based design optimization to provide theoretical support and technical references for glass selection and transparent envelope design in near-zero energy buildings in central-southern Anhui. Full article

Within the scope of this article is the presentation of a modelling and measurement approach for the effects of roof greenings and the application of the approach to evaluate the influence of roof greenings upon the thermal conditions inside a typical residential building. It is shown that overheating in summer can be reduced, and thermal comfort for inhabitants can be increased. The cooling is caused by the transpiration of plants and by the evaporation of water from the substrate. Other relevant physical effects are the shading of plants and the increase in the heat capacity of the building. In state-of-the-art buildings, a layer with a high insulating effect is incorporated into the envelope. This leads to the effect that a huge fraction of the cooling power is taken from the outside of the building and only a smaller part is taken from the inside. In order to mitigate this decoupling, a hydraulic connection between the greening and the interior of the building is introduced. To evaluate the effect of the inside cooling, the difference in the number of yearly hours with overheating in residential buildings is estimated. In addition, the reduction in energy demand for the climatisation of a typical residential building is calculated. The used methods are as follows: (1) Performance of laboratory and free field measurements. (2) Simulation of a typical residential building, using a validated approach. In summary, it can be said that green roofs, in particular with hydraulic connections, can significantly increase the interior thermal comfort and potentially reduce the energy required for air conditioning. Full article

Ultra-wideband (UWB) technology is a crucial facilitator for high-data-rate wireless communication due to its extensive frequency spectrum and low power consumption. Simultaneously, multiple-input multiple-output (MIMO) systems have garnered considerable attention owing to their capability to enhance channel capacity and link dependability. This article discusses the development of small, high-performance MIMO UWB antennas with mutual suppression capabilities to fully use the benefits of both technologies. Additionally, the suggested antenna features a straightforward design and dual-band-notched characteristics. The antenna structure includes two radiating elements measuring 85 × 45 mm². These elements use a rectangular patch provided by a coplanar waveguide (CPW). Double U-shaped slots are incorporated into the rectangular patch to introduce dual-band-notched properties, which help mitigate interference from WiMAX and WLAN communication systems. The antenna is fabricated on a Mylar^® polyester film substrate of 0.3 mm in thickness, with a dielectric constant of 3.2. According to the measurement results, the suggested antenna functions efficiently across the frequency spectrum of 2.29 to 20 GHz, with excellent impedance matching throughout the bandwidth. Furthermore, it provides dual-band-notched coverage at 3.08–3.8 GHz for WiMAX and 4.98–5.89 GHz for WLAN. The antenna exhibits impressive performance, including favorable radiation attributes, consistent gain, and little mutual coupling (less than −20 dB). Additionally, the envelope correlation coefficient (ECC) is extremely low (ECC < 0.01) across the working bandwidth, which indicates excellent UWB MIMO performance. This paper offers an appropriate design methodology for future flexible and compact UWB MIMO systems that can serve as interference-resilient antennas for next-generation wireless applications. Full article

This study proposes a dual-element wideband circularly polarized (CP) slot-integrated multiple-input multiple-output (MIMO) antenna with an X-notch square-shaped artificial magnetic conductor (AMC) for dedicated short-range communications (DSRC) applications. The proposed antenna design consists of two substrate layers separated by an air gap. The upper layer features a dual-element coplanar waveguide-fed slot antenna and a defected ground structure decoupling isolator, while the lower layer comprises an 8 × 8 array of X-notch square-shaped elemental units, functioning as an AMC reflector. Characteristic mode analysis shows that circular polarization is produced by the dominant orthogonal mode pair (modes J₅ and J₆), whose modal significance exceeds 0.92 and whose characteristic angle separation is 82° around the 5.9 GHz DSRC band. An I-shaped slot embedded in the ground plane of the upper layer serves as a defected ground structure isolator to suppress mutual coupling between antenna elements. Meanwhile, the X-notch square AMC reflector enhances radiation characteristics and antenna gain. The measured return loss bandwidth and axial ratio bandwidth are 32% (4.72–6.61 GHz) and 21.18% (5.2–6.45 GHz), respectively. The dual-element antenna scheme achieves high isolation exceeding 19 dB, with a maximum gain of 8.6 dBic at 5.9 GHz. The envelop correlation coefficient remains below 0.003, while the diversity gain exceeds 9.98 dB. Full article