Search Results (17)

Lake eutrophication, often driving harmful algal blooms (HABs) and ecosystem degradation, involves complex biogeochemical shifts within sediments. Changes in the sedimentary dissolved organic matter (DOM) composition during transitions from macrophyte to algal dominance are thought to critically regulate internal phosphorus (P) loading, yet the underlying mechanisms, especially in vulnerable plateau lakes like Qilu Lake, require further elucidation. This study investigated the coupled cycling of carbon (C) and P in response to historical ecosystem succession and anthropogenic activities using a 0–24 cm sediment core from Qilu Lake. We analyzed the total organic carbon (TOC), total phosphorus (TP), sequential P fractions, and DOM fluorescence characteristics (EEM-PARAFAC), integrated with chronological series data. The results revealed an asynchronous vertical distribution of TOC and TP, reflecting the shift from a submerged macrophyte-dominated, oligotrophic state (pre-1980s; high TOC, low TP, stable Ca-P dominance) to an algae-dominated, eutrophic state. The eutrophication period (~1980s–2010s) showed high TP accumulation (Ca-P and NaOH85 °C-P enrichment), despite a relatively low TOC (due to rapid mineralization), while recent surface sediments (post-2010s) exhibited a high TOC, but a lower TP following input controls. Concurrently, the DOM composition shifted from microbial humic-like dominance (C1) in deeper sediments to protein-like dominance (C3) near the surface. This study demonstrates that the ecosystem shift significantly regulates P speciation and mobility by altering sedimentary DOM abundance and chemical characteristics (e.g., protein-like DOM correlating negatively with Ca-P), reinforcing a positive feedback mechanism that sustains internal P loading and potentially exacerbates HABs. DOM molecular characteristics emerged as a key factor controlling the internal P cycle in Qilu Lake, providing critical insights for managing eutrophication in plateau lakes. Full article

Biochar is considered a promising amendment for improving phosphorus (P) availability in agricultural soils; however, its effects on the chemical transformation and long-term immobilization of P in submerged soils across soil depth and over time remain unclear. This study conducted a 98-day column incubation experiment to investigate the effects of rice straw biochar (RSB) on the spatial and temporal dynamics of iron (Fe) and P under soil submergence. Soils with and without biochar addition were mixed with water homogeneously and then added into each PVC column with an additional standing water layer above the soil surface. The results revealed a two-stage shift in soil redox potential (Eh), with more rapid changes observed at deeper depths. RSB addition accelerated the decline in Eh and increased the soil pH. The rise in pH by submergence and biochar addition promoted the release of soluble and exchangeable P from soil to pore water during incubation. Ca-associated P precipitation and re-adsorption resulted in relatively low phosphate concentrations in pore water. RSB addition increased P availability in the early stage by releasing soluble and exchangeable P and promoting phosphate desorption through pH elevation, which increased the negative surface charge of soil constituents, thereby reducing their affinity for phosphate and enhancing its release into the pore water. However, prolonged submergence led to the transformation of soluble and exchangeable P into more stable Ca-P precipitates, limiting long-term P availability. These findings provide new insights into the temporal and spatial dynamics of P in submerged soils and highlight the short-term benefits and long-term limitations of biochar for sustaining P availability in paddy rice systems. Full article

The present study examined the effect of different cap management techniques, maceration length, and ethanol levels through chaptalization on the chemical and sensory composition of Syrah wines from the Edna Valley AVA in California. Punch down wines had significantly higher anthocyanins, tannins, and total polymeric pigments compared to all other treatments. In terms of volatile chemistry, the submerged cap wines tended to have a higher concentration of esters and terpenes compared to the other treatments. Additionally, ethanol levels were more impactful on the chemical composition of the wines. As expected, chaptalized wines had significantly higher ethanol and glucose + fructose levels but also tended to have higher concentrations of esters and terpenes. Sensory evaluation was done through a modification of the Pivot© Profile method using an expert panel of winemakers (n = 15). The results suggested that cap management and the maceration length were more impactful on the sensory profile than the ethanol level whereby each cap management and maceration length treatment had a distinctive profile. As such, the punch down, chaptalized wines showed higher purple hue and color saturation attributes, blueberry orthonasal aromas as well as jammy and black fruit retronasal aromas. Submerged cap wines were associated with significantly meatier orthonasal aromas and reductive retronasal aromas. Extended maceration wines were characterized by more jammy orthonasal aromas and dried fruit retronasal aromas. However, within the extended maceration wines, the effect of chaptalization was apparent whereby the chaptalized wines showed more acetaldehyde aromas while the non-chaptalized wines were characterized by more herbal aromas. Full article

Fast high-temperature gas-phase reactions occurring in the limited space of the arc cavity in the submerged arc welding (SAW) process limit the study of specific gas-phase behaviours. A low-temperature experimental method is applied to investigate gas-phase reactions in the reaction of oxy-fluoride slag with Al-Fe-Ni metal powders. The presence of nano-strands in the slag cavities confirms the vaporisation and re-condensation of gasses. Ti is the main element in nano-strands, although some nano-strands also contain Al-Mg-Si-Na oxy-fluoride. Nano-strand end-caps contain Mn-Fe-Si fluoride, and some contain Ni. The Ni in nano-strand end-caps is sourced from the added Ni powder and indicates gas-phase transfer. The Ti in the nano-strands is sourced from the flux. Themochemistry calculations identify KAlF₄, TiF₃, NaAlF₄, SiF₄, AlF₃, SiF₃, and Na in the gas phase. Increased Al reaction results in decreased TiF₃ in the gas phase, likely due to the displacement of Ti from TiF₃, resulting in the gas-phase transfer of Ti from the flux. Comparative diffusion flux calculations support Ti nano-strand formation via the vaporisation of TiF₃ and the re-condensation of Ti. The low-temperature simulation experiment applied here can be used to study the gas reaction behaviour in the reaction of oxy-fluoride flux with metal powders. Full article

The submerged arc welding (SAW) process is operated at high temperatures, up to 2500 °C, in the arc cavity formed by molten oxy-fluoride flux (slag). These high arc cavity temperatures and the complex interaction of gas–slag–metal reactions in a small space below the arc render the study of specific chemical interactions difficult. The importance of gas phase reactions in the arc cavity of the SAW process is well established. A low-temperature (1350 °C) experimental method was applied to simulate and study the vaporisation and re-condensation behaviour of the gas species emanating from oxy-fluoride flux. Energy dispersive X-ray spectroscopy (EDX) analyses and reaction thermochemistry calculations were combined to explain the role of Al as a de-oxidiser element in gas phase chemistry and, consequently, in nano-strand formation reactions. EDX element maps showed that the nano-strands contain elemental Ti only, and the nano-strand end-caps contain Co-Mn-Fe fluoride. This indicates a sequence of condensation reactions, as Ti in the gas phase is re-condensed first to form the nano-strands and the end-caps formed from subsequent re-condensation of Co-Mn-Fe fluorides. The nano-strand diameters are approximately 120 nm to 360 nm. The end-cap diameter typically matches the nano-strand diameter. Thermochemical calculations in terms of simple reactions confirm the likely formation of the nanofeatures from the gas phase species due to the Al displacement of metals from their metal fluoride gas species according to the reaction: yAl + xMF_y ↔ xM + yAlF_x. The gas–slag–metal equilibrium model shows that TiO₂ in the flux is transformed into TiF₃ gas. Formation of Ti nano-strands is possible via displacement of Ti from TiF₃ by Al to form Al-fluoride gas. Full article

This study aimed to analyze the morphology of bone graft granules, the presence of granule demineralization, and bone morphology in retrieved human maxillary sinus bone graft biopsies. Healthy patients underwent sinus bone augmentation using lateral access. Two different dimensions of the antrostomy were performed, a 4 mm or 8 mm height. After 6 months, all sites received one implant using a flap technique, crestal positioning, and submerged healing. Implant biopsies were retrieved after 3 months and were histologically processed. The ESEM analysis was performed on the entire portion of the peri-implant bone (up to 750 µm from the implant thread). Three different regions of interest (ROIs) were selected: the coronal, middle, and apical portions of the implant. In these areas, EDX was performed, and calcium (Ca), phosphate (P), nitrogen (N), and their atomic ratios (Ca/P, Ca/N, and P/N) were calculated. Different bone tissue electron-dense areas were detected through grayscale intensity quantification of ESEM images with different organic (N) or inorganic (Ca,P) compositions. A total of 16 biopsies from 16 healthy patients were analyzed. Bone graft granules were mostly detected in the apical ROI. New bone tissue bridges were detected in the apical and middle ROI. These structures, with lower Ca/N and P/N ratios, were connected and enveloped the bone graft granules. Cortical ROI revealed the most mineralized bone tissue. Conclusions: After 9 months, bone graft resorption was only partially completed and new bone tissue appeared less mineralized in the middle and apical ROI than in the coronal ROI. Full article

The newly collected seismic data and the existing drilling data provide a good opportunity to better understand the carbonate platform distribution characteristics and the hydrocarbon resource potential in the Xisha sea area of the South China Sea. Based on the seismic data and the reflection characteristics of the carbonate platform’s edge, three boundary indicators were established: abrupt lithological interfaces, fault interfaces, and tidal channels. Combined with the regional geological settings, its spatial and temporal distribution was clearly identified for the first time. The development of the Miocene carbonate platform in the Xisha sea area is divided into six phases, which are further assigned to three evolutionary stages: the bloom stage, the recession stage, and the submerged stage. The sedimentary facies belt of the carbonate platform in each stage is well developed, and the reefs are mainly distributed on the west and southwest edges of the platform. The analysis of the data indicates that the area of the reef and carbonate platform reached 80,000 km² during the mature period, followed by a retreat period where the scale decreased with the platform’s decline. The Miocene carbonate rocks in the Xisha sea area are widely distributed. They have experienced multiple periods of exposure and infiltration, which further improved the quality of their physical properties for hydrocarbon reservoirs. According to the regional hydrocarbon geological conditions in this area—including the source rock, migration system and the capping layer—the hydrocarbon accumulation potential is preliminarily discussed in this paper. A reservoir model of the reef and carbonate platform is established, which is proposed as typical characteristics of “lower generation, upper accumulation”. It is pointed out that the carbonate platform in the Xisha sea area adjacent to the Huaguang Sag in the Qiongdongnan Basin and the northern Zhongjiannan Basin is a potential area for oil and gas exploration. Full article

Two innovative soft maceration techniques of vinification based on red grape Cabernet Sauvignon were compared in 2020 and 2021 vintages with the most used system of maceration (pump-over and delestage) in today’s wine sector in order to verify the efficiency in polyphenol extraction and fermentation rate. Fermentation kinetics and final wine characteristics were evaluated as the main parameters for comparing the systems. The AIR MIX (AIRMIXING M.I.™) technique is based on the use of a fixed sequential small injections of compressed air (3 jets) from the bottom of the tank, aimed at creating waves (resonance waves) able to prevent the cap formation. The ADCF (NECTAR-ADCF™) technique uses the overpressure produced by carbon dioxide in the wine tank during alcoholic fermentation to keep the cap submerged and to favor its disruption by the CO₂ outside release through a valve. As a reference, the control vinification consisted of the use of “delestage” and pump-over to facilitate the extraction and good management of the cap. ADCF, at the end, extracted a greater quantity of polyphenols and anthocyanins. AIR MIX speeded up the fermentation, which ended 4–7 days before the control and, initially, provoked a greater extraction of phenols and anthocyanins as ADCF. By the end, the concentration of polyphenols and anthocyanins was the highest in ADCF, followed by control and AIR MIX approximately at the same amount. The control wine had a slightly higher volatile acidity. AIR MIX consumed more than 60% less energy because the nonuse of pump-over and delestage, and also, no personnel was requested. Full article

Salt preparation via a solid-state reaction offers a solution to challenges posed by current pharmaceutical research, which include combining development of novel forms of active pharmaceutical ingredients with greener, sustainable synthesis. This work investigated in detail the mechanism of salt formation between propranolol (PRO) and capric acid (CAP) and explored the solid eutectic phases comprising this salt, propranolol caprate (PRC). The salt structure was solved by X-ray diffraction, and the properties in the crystalline and supercooled states were fully characterised using thermal analysis, nuclear magnetic resonance, Fourier-transform infrared spectroscopy and broadband dielectric spectroscopy (BDS). PRC forms via a submerged eutectic phase composed of PRO and CAP, below room temperature, by mechanochemistry without an extra input of energy. Two other solid eutectic phases are composed of PRC and either CAP or PRO, at 0.28 and 0.82 mol fraction of PRO, respectively. BDS indicated that the supercooled PRC has ionic character, whereas the supercooled PRC-PRO eutectic had predominantly non-ionic properties despite comprising the salt. In conclusion, knowledge of the mechanism of formation of multicomponent systems can help in designing more sustainable pharmaceutical processes. Full article

Eutrophication often results in the loss of submerged vegetation in shallow lakes and turns the lake to be a turbid state. Recovery of submerged macrophytes is the key in the restoration of shallow eutrophic lakes to create a clear water state. However, internal loading control was considered as the critical process for the recovery of submerged macrophytes in shallow lakes after the external nutrient reduction. Phoslock^® (Lanthanum modified bentonite) is a useful passivation material in controlling the internal loadings (release of phosphorus from the sediments), which was applied to restore the eutrophic lakes. However, the effects of Phoslock^® on the growth and life strategies of submerged macrophytes are less focused so far. In the present study, we studied the responses in the growth and morphological characteristics of Myriophyllum spicatum to the addition of Phoslock^® to the sediments. Our results showed that the addition of Phoslock^® significantly decreased the contents of bioavailable forms of phosphorus in the sediments, such as redox-sensitive phosphorus bound to Fe and Mn compounds (BD–P), phosphorus bound to aluminum (Al–P) and organic phosphorus (Org–P). However, the concentration of the non-bioavailable forms of phosphorus in the sediments, such as calcium bound phosphorus (Ca–P), increased significantly in the Phoslock^® treatments compared with the controls. At the end of the experiments, the total biomass, aboveground biomass and relative growth rate (RGR) of M. spicatum decreased significantly in the Phoslock^® mesocosms compared with the controls. In contrast, the wet root biomass, root–shoot biomass ratio, root numbers and root length of M. spicatum were significantly higher in the Phoslock^® treatments than that in the controls. Our results indicated that the growth of M. spicatum was suppressed by the addition of Phoslock^®, and thus the biomass was decreased; however, the increase of root biomass might be beneficial to the inhibition of phosphorus release and resuspension of sediments and to the restoration of the lake ecosystem. Full article

Bermuda is an Eocene age volcanic island in the western North Atlantic, entirely capped by Pleistocene eolian limestone. The oldest and most highly karstified limestone is a 2 km² outcrop of the Walsingham Formation containing most of the island’s 150+ caves. Extensive networks of submerged cave passageways, flooded by saltwater, extend under the island. In the early 1980s, cave divers initially discovered an exceptionally rich and diverse anchialine community inhabiting deeper sections of the caves. The fauna inhabiting caves in the Walsingham Tract consists of 78 described species of cave-dwelling invertebrates, including 63 stygobionts and 15 stygophiles. Thus, it represents one of the world’s top hotspots of subterranean biodiversity. Of the anchialine fauna, 65 of the 78 species are endemic to Bermuda, while 66 of the 78 are crustaceans. The majority of the cave species are limited in their distribution to just one or only a few adjacent caves. Due to Bermuda’s high population density, water pollution, construction, limestone quarries, and trash dumping produce severe pressures on cave fauna and groundwater health. Consequently, the IUCN Red List includes 25 of Bermuda’s stygobiont species as critically endangered. Full article

Surface-associated multicellular assemblage is an important bacterial trait to withstand harsh environmental conditions. Bacillus subtilis is one of the most studied Gram-positive bacteria, serving as a model for the study of genetic pathways involved in the different steps of 3D biofilm formation. B. subtilis biofilm studies have mainly focused on pellicle formation at the air-liquid interface or complex macrocolonies formed on nutritive agar. However, only few studies focus on the genetic features of B. subtilis submerged biofilm formation and their link with other multicellular models at the air interface. NDmed, an undomesticated B. subtilis strain isolated from a hospital, has demonstrated the ability to produce highly structured immersed biofilms when compared to strains classically used for studying B. subtilis biofilms. In this contribution, we have conducted a multi-culturing comparison (between macrocolony, swarming, pellicle, and submerged biofilm) of B. subtilis multicellular communities using the NDmed strain and mutated derivatives for genes shown to be required for motility and biofilm formation in pellicle and macrocolony models. For the 15 mutated NDmed strains studied, all showed an altered phenotype for at least one of the different culture laboratory assays. Mutation of genes involved in matrix production (i.e., tasA, epsA-O, cap, ypqP) caused a negative impact on all biofilm phenotypes but favored swarming motility on semi-solid surfaces. Mutation of bslA, a gene coding for an amphiphilic protein, affected the stability of the pellicle at the air-liquid interface with no impact on the submerged biofilm model. Moreover, mutation of lytF, an autolysin gene required for cell separation, had a greater effect on the submerged biofilm model than that formed at aerial level, opposite to the observation for lytABC mutant. In addition, B. subtilis NDmed with sinR mutation formed wrinkled macrocolony, less than that formed by the wild type, but was unable to form neither thick pellicle nor structured submerged biofilm. The results are discussed in terms of the relevancy to determine whether genes involved in colony and pellicle formation also govern submerged biofilm formation, by regarding the specificities in each model. Full article

A Wheeler method for the evaluation of the radiation efficiency of submerged antennas within lossy media is presented and demonstrated for the first time in the literature. Extensive investigations have been devised by empirical and simulation methods. Normal-mode helical antenna (NMHA) was first designed and fabricated to exemplify a real-life application at the UHF band (0.3 to 3 GHz). The antenna under test (AUT) was evaluated within an artificial lossy material using a series of Wheeler caps featuring different radii to study the validity of this method. The error between the experimental and simulation radiation efficiency is below 3% near the theoretical radian length. The presented measurement method of radiation efficiency without any essential measurement facilities or accessories could be a promising candidate for fast and accurate evaluation for any wire-type antenna submerged within lossy media. Full article

Silica (SiO₂)/chitosan (CS) composite aerogels are bioactive when they are submerged in simulated body fluid (SBF), causing the formation of bone-like hydroxyapatite (HAp) layer. Silica-based hybrid aerogels improve the elastic behavior, and the combined CS modifies the network entanglement as a crosslinking biopolymer. Tetraethoxysilane (TEOS)/CS is used as network precursors by employing a sol-gel method assisted with high power ultrasound (600 W). Upon gelation and aging, gels are dried in supercritical CO₂ to obtain monoliths. Thermograms provide information about the condensation of the remaining hydroxyl groups (400–700 °C). This step permits the evaluation of the hydroxyl group’s content of 2 to 5 OH nm⁻². The formed Si-OH groups act as the inductor of apatite crystal nucleation in SBF. The N₂ physisorption isotherms show a hysteresis loop of type H3, characteristic to good interconnected porosity, which facilitates both the bioactivity and the adhesion of osteoblasts cells. After two weeks of immersion in SBF, a layer of HAp microcrystals develops on the surface with a stoichiometric Ca/P molar ratio of 1.67 with spherulite morphology and uniform sizes of 6 μm. This fact asserts the bioactive behavior of these hybrid aerogels. Osteoblasts are cultured on the selected samples and immunolabeled for cytoskeletal and focal adhesion expression related to scaffold nanostructure and composition. The initial osteoconductive response observes points to a great potential of tissue engineering for the designed composite aerogels. Full article

This experiment analysed the operation of submerged and dripping biological filters with three types of filling: commercial fitting HXF12KLL (CF), two innovative polypropylene aggregates (PPA) and polyethylene screw caps for PET bottles (PSC). The experiment determined the time needed to reach full filter functionality at the maturation stage, the time needed to start successive stages of the nitrification process and the maximum concentration of each nitrogen compound in water in the recirculation systems. The filter operation characteristics after the maturation stage were also examined. These issues are crucial during the preparation and launch of new aquaculture facilities. A literature analysis indicated that the ability of biological filters to oxidise nitrogen compounds is affected by a number of factors. Studies conducted at various centres have covered selected aspects and factors affecting the effectiveness of biological filters. During this study, the model fish common carp (Cyprinus carpio) was used. The current experiment involved examination of biological filter maturation and operation during the carp fry rearing stage, which allowed the biofilter operation characteristics to be determined. At the third day of the experiment, the ammonium concentration reached approximately 3 mg NH₄-N/dm³. It remained at this level for 10 days and later decreased below 0.25 mg NH₄-N/dm³. The maximum nitrite concentration ranged from 11.7 mg/dm³ to 20.9 mg NO₂-N/dm³ within 9 to 20 days and later decreased with time. Nitrate concentrations were seen to increase during the experiment. The all applied biofilter media showed possibility to be used in commercial aquaculture systems. Full article