Search Results (264)

The intestinal barrier governs organismal health through nutrient absorption, microbial homeostasis, and immune surveillance. While calorie restriction (CR) enhances metabolic health, the molecular mechanisms underlying its beneficial effects on gut integrity remain unclear. Here, we demonstrate that the aryl hydrocarbon receptor (AHR), a conserved xenobiotic sensor and metabolic regulator, is essential for CR-mediated improvements in intestinal function. Using Caenorhabditis elegans (C. elegans), we subjected wild-type (N2) and AHR-deficient strains (CZ2485 and ZG24) to ad libitum feeding (AL), intermittent fasting (IF), or complete food deprivation (FD). In wild-type animals, intermittent fasting markedly reduced intestinal permeability and bacterial burden while enhancing mitochondrial function and reducing reactive oxygen species. Complete food deprivation conferred modest benefits. Remarkably, these protective effects were severely compromised in AHR mutants, which exhibited increased gut leakage, bacterial colonization, and mitochondrial oxidative stress under fasting conditions. These findings establish AHR as a critical mediator of fasting-induced intestinal resilience, revealing a previously unrecognized regulatory axis linking metabolic sensing to gut barrier homeostasis. Our work illuminates fundamental mechanisms through which calorie restriction promotes gastrointestinal health and identifies AHR-dependent pathways as promising therapeutic targets for metabolic and inflammatory distress affecting the gut–systemic interface. Full article

The mechanism by which quorum sensing (QS) enhances stress resistance in enterohemorrhagic Escherichia coli (E. coli) O157:H7 remains unclear. We employed optimized exogenous QS signal N-acyl-homoserinelactones (AHL) (100 μM 3-oxo-C6-AHL, 2 h) in EHEC O157:H7 strain EDL933, which was validated with endogenous yenI-derived AHL, to investigate QS-mediated protection against acid stress. RNA-seq transcriptomics identified key upregulated genes (e.g., rmf). Functional validation using isogenic rmf knockout mutants generated via λ-Red demonstrated abolished stress resistance and pan-stress vulnerability. Mechanistic studies employing qRT-PCR and stress survival assays established Ribosomal Hibernation Factor (RMF) as a non-redundant executor in a SdiA–RMF–RpoS axis, which activates ribosomal dormancy and SOS response to enhance EHEC survival under diverse stresses. For the first time, we define ribosomal hibernation as the core adaptive strategy linking QS to pathogen resilience, providing crucial mechanistic insights for developing EHEC control measures against foodborne threats. Full article

In this research, a single composite-type stretchable triboelectric nanogenerator (TENG) is proposed for efficient energy harvesting and handwriting recognition. The composite TENGs were fabricated by blending dielectric barium titanate (BT) and conductive carbon nanotubes (CNTs) in varying amounts into a styrene–butadiene rubber matrix. The energy harvesting efficiency depends on the type and amount of fillers, as well as their dispersion within the matrix. Stearic acid modification of BT enables near-nanoscale filler distribution, resulting in high energy conversion efficiencies. The composite achieved power efficiency, power density, charge efficiency, and charge density values of 1.127 nW/N, 8.258 mW/m³, 0.146 nC/N, and 1.072 mC/m³, respectively, under only 2% cyclic compressive strain at 0.85 Hz. The material performs better at low stress–strain ranges, exhibiting higher charge efficiency. The generated charge in the TENG composite is well correlated with the compressive stress, which provides a minimum activation pressure of 0.144 kPa, making it suitable for low-pressure sensing applications. A flat composite with dimensions of 0.02 × 6 × 5 cm³ can produce a power density of 26.04 W/m³, a charge density of 0.205 mC/m³, and an output voltage of 10 V from a single hand pat. The rubber composite also demonstrates high accuracy in handwriting recognition across different individuals, with clear differences in sensitivity curves. Repeated attempts by the same person show minimal deviation (<5%) in writing time. Additionally, the presence of reinforcing fillers enhances mechanical strength and durability, making the composite suitable for long-term cyclic energy harvesting and wearable sensor applications. Full article

Background: Antibiotics at sub-inhibitory concentrations can rewire bacterial regulatory networks, impacting virulence. Objective: The way that exposure to selected antibiotics (ciprofloxacin, amikacin, azithromycin, ceftazidime, and meropenem) below their minimum inhibitory concentration (sub-MIC) modulates the physiology of Pseudomonas aeruginosa is examined in this study using growth-phase-resolved analysis. Methods: Standard P. aeruginosa strain cultures were exposed to ¼ and ½ MIC to determine the growth kinetics under antibiotic stress. The study measured protease and pyocyanin production and the expression level of important quorum sensing and virulence genes (lasI/R, rhlI/R, pqsR/A, and phzA) at different growth phases. Results: Meropenem produced the most noticeable growth suppression at ½ MIC. Sub-MIC antibiotics did not completely stop growth, but caused distinct, dose-dependent changes. Azithromycin eliminated protease activity in all phases and had a biphasic effect on pyocyanin. Ciprofloxacin consistently inhibited both pyocyanin and protease in all phases. The effects of amikacin varied by phase and dose, while β-lactams markedly increased pyocyanin production during the log phase. In contrast to the plateau phase, when expression was often downregulated or unchanged, most quorum-sensing- and virulence-associated genes showed significant upregulation during the death phase under sub-MIC exposure. Conclusions: These findings indicate that sub-MIC antibiotics act as biochemical signal modulators, preserving stress-adapted sub-populations that, in late growth phases, activate quorum sensing and stress tolerance pathways. Full article

In this study, a new fibre combination for an interlayer hybrid fibre-reinforced polymer laminate was investigated to achieve pseudo-ductile behaviour in tensile tests. The chosen high-strain fibre for this purpose was S-Glass, and the low-strain fibre was flax. These materials were chosen because of their relatively low environmental impact compared to carbon/carbon and carbon/glass hybrids. An analytical model was used to find an ideal combination of the two materials. With that model, the expected stress–strain relation could also be predicted analytically. The modelling was based on preliminary tensile tests of the two basic components investigated in this research: unidirectional laminates reinforced with either flax fibres or S-Glass fibres. Hybrid specimens were then designed, produced in a heat-assisted pressing process, and subjected to tensile tests. The strain measurement was performed using distributed fibre optic sensing. Ultimately, it was possible to obtain repeatable pseudo-ductile stress–strain behaviour with the chosen hybrid when the specimens were subjected to quasi-static uniaxial tension in the direction of the fibres. The intended damage-mode, consisting of a controlled delamination at the flax-fibre/glass-fibre interface after the flax fibres failed, followed by a load transfer to the glass fibre layers, was successfully achieved. The pseudo-ductile strain averaged 0.52% with a standard deviation of 0.09%, and the average load reserve after delamination was 145.5 MPa with a standard deviation of 48.5 MPa. The integrated fibre optic sensors allowed us to monitor and verify the damage process with increasing strain and load. Finally, the analytical model was compared to the measurements and was partially modified by neglecting the Weibull strength distribution of the high-strain material. Full article

COVID-19 school closures forced German high-school graduates (Abitur 2022 cohort) to prepare for their final examinations with lengthy learning times at home. Guided by transactional stress theory, we tested how personal resources—self-regulated learning (SRL) skills and academic self-efficacy—and contextual resources—perceived teacher support and teacher digital competence—jointly predicted perceived stress during exam preparation. A cross-sectional online survey (June–July 2022) yielded complete data from N = 2379 students (68% female; Mage = 18.3). Six latent constructs were measured with 23 items and showed adequate reliability (0.71 ≤ α/ω ≤ 0.89). A six-factor CFA fit the data acceptably (CFI = 0.909, RMSEA = 0.064). The structural equation model (CFI = 0.935, RMSEA = 0.064) explained 35% of the variance in stress and 23% of the variance in SRL—action. Academic self-efficacy (β = −0.31, p < 0.001), perceived support (β = −0.28, p < 0.001), teacher digital competence (β = −0.08, p < 0.001), COVID-19 learning disruptions (β = +0.13, p < 0.001), and gender (male = 0.32 SD lower stress, p < 0.001) had direct effects on stress. SRL—action’s direct path was small and non-significant (β = −0.02). Teacher digital competence also reduced stress indirectly through greater perceived support (standardized indirect β = −0.11, p < 0.001). The results highlight self-efficacy and perceived instructional support as the most potent buffers of pandemic-related stress, whereas cancelled lessons added strain. Boosting teachers’ digital pedagogical skills has a dual payoff—raising students’ sense of support and lowering their stress. Full article

This paper presents an analysis of the time-dependent response of a spherical container to internal pressure that increases over time. The wall of the container is relatively thin, in the sense that the wall thickness is negligible in comparison to the container’s radius. The wall is composed of three layers. The two surface layers of the wall are identical, i.e., they are made from the same material and have the same thickness. One of the most important features regarding the response of the wall layers is the non-linear creep. The stresses and strains are determined, and their relationships with the parameters of the layers are studied. Full article

Wearable sensors have rapidly advanced, enabling applications such as human activity monitoring, electronic skin, and biomimetic robotics. To meet the growing demands of these applications, multifunctional sensing has become essential for wearable devices. However, most existing studies predominantly focus on enhancing single-function sensing capabilities. This study introduces a multifunctional sensor that combines high stretchability for strain and pressure detection with ultraviolet (UV) sensing capability. To achieve simultaneous detection of strain, pressure, and UV light, a multi-sensing approach was employed: a capacitive method for strain and pressure detections and a resistive method utilizing a pn-heterojunction diode for UV detection. In the capacitive method, polyaniline (PANI) served as parallel-plate electrodes, while silicon-based elastomer acted as the dielectric layer. This configuration enabled up to 100% elongation and enhanced operational stability through encapsulation. The sensor demonstrated a strong linear relationship between capacitance value changes reasonably based on the area of PANI, and showed a good linearity with an R-squared value of 0.9918. It also detected pressure across a wide range, from low (0.4 kPa) to high (9.4 kPa). Furthermore, for wearable applications, the sensor reliably captured capacitance variations during finger bending at different angles. For UV detection, a pn-heterojunction diode composed of p-type silicon and n-type zinc oxide nanorods exhibited a rapid response time of 6.1 s and an on/off ratio of 13.8 at −10 V. Durability under 100% tensile strain was confirmed through Von Mises stress calculations using finite element modeling. Overall, this multifunctional sensor offers significant potential for a variety of applications, including human motion detection, wearable technology, and robotics. Full article

The development of biocompatible, conductive hydrogels via direct ink writing (DIW) has gained increasing attention for strain sensor applications. In this work, a hydrogel matrix composed of polyvinyl alcohol (PVA) and κ-carrageenan (KC) was formulated and enhanced with polyvinylidene fluoride (PVDF) and silver nanoparticles (AgNPs) to impart piezoelectric properties. The ink formulation was optimized to achieve shear-thinning and thixotropic recovery behavior, ensuring printability through extrusion-based 3D printing. The resulting hydrogels exhibited high water uptake (~280–300%) and retained mechanical integrity. Rheological assessments showed that increasing PVDF content improved stiffness without compromising printability. Electrical characterization demonstrated that AgNPs were essential for generating piezoelectric signals under mechanical stress, as PVDF alone was insufficient. While AgNPs did not significantly alter the crystalline phase distribution of PVDF, they enhanced conductivity and signal responsiveness. XRD and SEM-EDX analyses confirmed the presence and uneven distribution of AgNPs within the hydrogel. The optimized ink formulation (5% PVA, 0.94% KC, 6% PVDF) enabled the successful fabrication of functional sensors, highlighting the material’s strong potential for use in wearable or biomedical strain-sensing applications. Full article

Streptococcus agalactiae (GBS) is a major pathogen causing mastitis in dairy cows while causing oxidative stress. Matrine is an alkaloid compound extracted from the roots of Sophora flavescens, a plant used in traditional Chinese medicine. It possesses antioxidant, immunomodulatory, anti-inflammatory, and pro-apoptotic properties. The aim of this study was to investigate the regulatory effects of matrine on the virulence of the ATCC strain (ATCC13813) and clinical GBS strains by transcriptome analysis and qRT-PCR validation. The results showed that the ABC transporter, peptidoglycan biosynthesis, and quorum-sensing pathways were significantly altered in ATCC (4 mg/mL) and GBS (12 mg/mL) strains after matrine treatment at MIC concentrations. Additionally, genes related to invasion and immune escape, including CylE, CAMP, ScpB, and CpsA, and genes related to the expression of adhesion and virulence factors, such as Bac, Lmb, PI2a, and PI2b, were significantly downregulated (p < 0.05). Overall, these data suggest that matrine effectively inhibits the virulence genes of GBS, thereby reducing immune evasion and infection by decreasing the synthesis of capsular polysaccharides and host cell adhesion. Full article

We present and demonstrate a highly birefringent fiber Bragg grating (Hi-Bi FBG) that was fabricated using a femtosecond laser to induce a sawtooth stress region near the FBG. The FBG is fabricated with a femtosecond laser point-by-point method, while the sawtooth stress region is generated in fiber cladding using the femtosecond laser along a sawtooth path. This sawtooth stressor can introduce an anisotropic and asymmetric refractive index profile in the cross-section of the fiber, resulting in additional birefringence up to 2.97 × 10⁻⁴ along the axial direction of the FBG. The central wavelengths of the Hi-Bi FBG at the fast and slow axes exhibit different sensitivities to temperature and strain, allowing simultaneous measurement of the strain and temperature by tracking the resonant wavelength shifts in the two axes. The experimental results show that the temperature sensitivities of the fast and slow axes are 10.32 pm/°C and 10.42 pm/°C, while the strain sensitivities are 0.91 pm/µε and 0.99 pm/µε. The accuracy of this proposed sensor in measuring strain and temperature is estimated to be 2.2 µε and 0.2 °C. This approach addresses the issue of cross-sensitivity between temperature and strain and offers some advantages of low cost, compact size, and significant potential for advancements in practical multi-parameter sensing applications. Full article

Psychrotrophic Morganella spp. is a typical histamine producer commonly found in seafood, exhibiting a high histamine-producing capacity. In this study, two strains of Morganella (GWT 902 and GWT 904) isolated from yellowfin tuna were subjected to phenotypic and genotypic characterization. Phenotypic analysis reveals differences in growth temperature, NaCl tolerance, and D-galactose fermentation capacity between the two strains. Notably, the histamine production capacity of GWT 902 is significantly higher than that of GWT 904 at 4 °C. The complete genome sequences of strains GWT 902 and GWT 904 were sequenced, identifying GWT 902 as Morganella psychrotolerans and GWT 904 as Morganella morganii subsp. sibonii. Genomic analysis confirms the presence of histidine decarboxylase gene clusters (hdcT1, hdc, hdcT2, hisRS) in both strains, and sequence alignment shows that the amino acid sequence similarity of histidine decarboxylase encoded by the hdc gene was 95.24%. Gene function analysis further identified genes associated with putrescine biosynthesis, sulfur metabolism, lipase and protease secretion, and detected key genes in quorum sensing (QS), stress adaptation, and antibiotic resistance. This study provides valuable insights into the taxonomic analysis of psychrotrophic Morganella spp. and contributes to the development of efficient strategies for preventing histamine formation in seafood. Full article

Mechanoluminescence (ML) involves light emission induced by mechanical stress, categorized into triboluminescence (TL), piezoluminescence (PL), sonoluminescence (SL), and triboelectrification-induced electroluminescence (TIEL). The most common is TL, in which crystal fracture generates opposing charges that excite surrounding molecules. In PL, applied pressure induces light emission via charge recombination. SL occurs in gas-saturated liquids under sudden pressure changes. TIEL has gained increasing attention as it operates without the need for asymmetric crystal structures or strain fields. However, conventional ML faces practical limitations due to its dependence on complex structures or strain fields. In contrast, contact-electro-luminescence (CEL) has emerged as a promising alternative, enabling luminol luminescence via charge transfer and reactive oxygen species generation through contact electrification (CE) between inert dielectrics and water. CEL provides a simpler and more versatile approach than traditional ML techniques, underscoring the pivotal role of charge-transfer processes. This perspective highlights the potential of CEL in expanding ML applications across sensing, energy conversion, and environmental monitoring. Full article

Addressing the critical technological needs for the real-time monitoring of stress distribution in mining areas, a new method for inverting goaf pressure using distributed optical fiber monitoring data is proposed. By coupling the key stratum fracture mechanics model with the subsidence trajectory function model, a theoretical model is established to accurately describe spatial stress evolution during coal mining. The model quantifies the relationship between goaf pressure changes and key stratum failures through a two-stage analysis of the subsidence process, based on distinct mechanical properties before and after key stratum fracture. Physical model experiments (3 m × 0.2 m × 1.1 m) using Brillouin Optical Time Domain Analysis (BOTDA) technology validated the proposed method, with comprehensive monitoring of key stratum deformations. By coupling the fracture mechanics model of the critical layer and the settlement trajectory function model, the dynamic transformation of the pre-fracture and post-fracture stages is realized, and the stress evolution can be monitored and predicted in real time. The results demonstrate spatial consistency between key stratum fracture locations and goaf peak stress positions. High-precision optical fiber sensing detected an ultimate strain threshold of 4000 με for key stratum failure, with pre-fracture strain measurements consistently below this threshold. The developed stress inversion formula successfully predicted pressure distribution patterns within the goaf, achieving real-time monitoring capabilities. Compared with the BPPS measurements, the deviation in the inverted data is less than 8.88%, the root mean square error (RMSE) is 0.98–1.20 in different propulsion stages, and the coefficient of determination (R²) is between 0.72 and 0.85. These findings provide a crucial theory for predicting peak stress evolution in mining areas, with implications for improving safety monitoring systems and optimizing mining operations. Full article

The plastic zone shields the crack tip from high stress and plays an important role in the fracture of structures. Determination of the plastic zone is a significant challenge in large-scale and complex structures. In the present work, a detection method using mechanochromic luminescent (MCL) sensing film has been proposed to detect the plastic zone near the crack tip. The deformation near the crack tip is converted into visible green fluorescence emission. A comprehensive post-processing protocol and a feature quantification scheme for fluorescence images are introduced. A significant correlation is obtained between the characteristic values of fluorescence images and the parameters of the plastic zone (i.e., maximum equivalent strain and plastic zone size), indicating that the fluorescence response provides effective characterization parameters within the forward model. The plastic zone parameters determined using the MRL-based method agree well with the results measured using the DIC method. This indicates that the plastic zone near the crack tip can be effectively analyzed by capturing loading conditions and fluorescence response. Full article