Search Results (7,514)

Dairy cows are economically significant ruminants in China, and the dairy industry is closely linked to food safety and the agricultural economy. However, various factors such as pathogenic microorganisms often lead to frequent diseases in dairy cows. Furthermore, as potential hosts for diverse viruses, dairy cows can harbor zoonotic pathogens, which pose a threat to public health. The Shaanxi–Gansu–Ningxia region boasts abundant natural resources and extensive pastures. It is a major animal husbandry base in Northwest China, and dairy farming plays a significant role in the local economy. However, research on dairy cow virus diversity in this region remains limited; epidemic prevention and control capabilities are constrained, and the risk of disease outbreaks is elevated. In this study, 790 dairy cow samples were collected from 13 large-scale farms and free-range households in the Shaanxi–Gansu–Ningxia region from 2021 to 2023. Sample types consisted of nasal and anal swabs. Six viral metagenomic libraries were constructed and analyzed using high-throughput sequencing and bioinformatics methods, leading to the identification of 51 viral families. These comprised 16 positive-sense single-stranded RNA virus families, one Retroviridae family, four double-stranded RNA virus families, 21 double-stranded DNA virus families, and nine single-stranded DNA virus families. Among these, RNA viruses were represented by families such as Astroviridae, Coronaviridae, Caliciviridae, Picornaviridae, and Picobirnaviridae; DNA viruses were primarily detected in Circoviridae, Papillomaviridae, Genomoviridae, and Smacoviridae. Alpha diversity analysis revealed no significant differences in viral diversity and abundance among the three regions (p > 0.05); however, significant differences were observed in the read counts and proportions of RNA and DNA viruses across the provinces. Phylogenetic analysis further indicated that viruses carried by dairy cows exhibit considerable genetic diversity and pose potential cross-species transmission risks. This study established a reference database for the dairy cow virome in the Shaanxi–Gansu–Ningxia region, elucidated the phylogenetic relationships of key viruses, and provided a scientific basis for future monitoring and prevention of dairy cow viruses. Full article

This paper investigates the in-plane bending stiffness of light timber-framed (LTF) and light steel-framed (LSF) wall elements with different sheathing materials (fibre-plaster board (FPB) and oriented-strand board (OSB)), focusing on the influence of the fastener spacing (s) on the wall elements’ structural response. The analytical model accounts for bending, shear, and slip deformations in the sheathing-to-frame connection, while boundary conditions are assumed to be rigid in accordance with the Eurocode 5 standard. The results indicate a strong dependence of global stiffness on fastener spacing. Increasing the fastener spacing from 37.5 mm to 300 mm reduced the racking stiffness by approximately 42% in LTF–FPB walls and by 31% in LSF–FPB walls. The highest stiffness was obtained for LSF–FPB wall elements (6514 N/mm), while the lowest stiffness was observed for LTF–OSB elements (1236 N/mm). LSF wall elements generally exhibited stiffness values approximately two times higher than comparable LTF systems, although both framing systems showed similar trends with increasing fastener spacing. This study provides a solid basis for the design and optimization of lightweight wall systems and supports the development of efficient structural solutions in both timber and steel construction. Full article

Although the long-term behavior of ground anchors depends fundamentally on interfacial behavior, the independent effect of the strand–grout interface on load loss has not been comprehensively investigated. This study establishes a physical model testing method that isolates the strand–grout interface and systematically investigates both short-term and long-term load loss behavior. Pull-out tests and long-term monitoring tests were conducted using grout uniaxial compressive strength ($q_u$ = 18–30 MPa) and bond length ($L_b$ = 900–1500 mm) as primary design variables. Long-term monitoring confirmed that prestress loss at the strand–grout interface is induced by the progressive pull-out displacement of the strand over time, following a logarithmic decay pattern. The load reduction coefficient n was significantly more sensitive to $L_b$ than to $q_u$; n increased sharply from 0.015 to 0.069 as $L_b$ decreased. Anchors with insufficient bond length exhibited secondary load reduction behavior that disrupted the stable log-linear decay, posing significant risk to long-term performance. Based on RMSE analysis of the fitted logarithmic model, a minimum monitoring period of approximately 50 days is recommended for reliable long-term prediction when bond length is adequate. These findings identify $q_u$ and $L_b$ as the governing parameters, providing a quantitative basis for optimizing prestress design and enhancing the long-term reliability of anchor systems. Full article

The expansion of remote work has transformed labour market conditions across the developed world, yet access to home-based work remains unequally distributed along occupational, sectoral, regional, and organisational lines. Post-pandemic evidence on the persistence of these inequalities is particularly scarce in Central and Eastern European economies, where historically low remote work prevalence, manufacturing-intensive industrial structures, and pronounced regional disparities create a distinctive structural context. Drawing on primary survey data collected from 390 employees in Slovakia in 2025, this study pursues two interrelated empirical goals: to identify the factors predicting a mismatch between the structural feasibility of working from home and its actual availability to employees, and to examine the determinants of experienced work discomfort. Binary logistic regression, multiple linear regression, and a battery of group difference tests were employed across the two analytical strands. The results reveal a pronounced capital–periphery gradient in remote work access, with employees outside the capital city facing dramatically higher odds of mismatch, and identify organisational support as the most practically actionable determinant of work discomfort. Notably, experiencing a mismatch between remote work feasibility and access was not associated with higher discomfort, a finding that challenges assumptions common in the Western European literature and points to the moderating role of contextual expectations in post-communist labour markets. The findings offer directly applicable evidence for employers seeking to reduce work-related strain through targeted support measures, and for policymakers designing regulatory frameworks to promote equitable access to flexible work arrangements across regions and sectors. Full article

Endogenous gene tagging using CRISPR has changed the understanding of the role played by different proteins due to the ability to track and study proteins in their natural state. With CRISPR-based gene tagging, it is possible to insert fluorescent, luminescent, epitope, affinity, and proximity labels into the target protein at its endogenous genomic location without affecting its physiological expression and dynamics. Here, we discuss the DNA-repair mechanisms employed in endogenous gene tagging, including homology-dependent repair, NHEJ-based integration, and alternative approaches that can be used with challenging cell types. Key aspects of efficient CRISPR tagging experiments are also described. Additionally, we review recent advances in the increasing array of protein tag technologies, including fluorescent proteins, split-reporter technologies, NanoLuc/HiBiT, peptide epitopes, and proximity biotinylation enzymes. Lastly, we review the scalability of endogenous tagging approaches using multiplex editing, atlas-scale proteome tagging, iPSC-based disease modeling, and drug discovery platforms for assessing target engagement, protein degradation, phenotype screening, and mechanism of action of compounds. Although difficult in primary and pluripotent cells, new methods based on avoiding double-strand breaks, such as prime editing, PASTE, and CRISPR associated transposases, will drive the future expansion of endogenous tagging approaches. Such developments firmly set up CRISPR gene tagging as a fundamental technology in quantitative cell biology and translational pharmacology. Full article

A novel sodium-oxide-fluxed slag is applied in the aluminothermic reduction of manganese ore. The slag’s high Al₂O₃ solubility facilitates the recycling of Al₂O₃ through hydrometallurgical processes, where NaAlO₂ serves as a water-leachable compound. Aluminothermic reduction is gaining renewed interest as an alternative processing route for the circular economy. In addition, CO₂ emissions in aluminium production via the electrochemical Hall–Héroult process can be reduced if the process electricity is sourced from non-fossil fuels. The unique Na₂O-fluxed MnO₂ ore formulation includes a small quantity of carbon reductant to ensure rapid pre-reduction to MnO. This approach negates the need for a pre-roasting step. Feed mixture variations with different collector metal additions (Si, Cr, Cu) were made to improve alloy–slag separation efficiency. The collector metals may influence the chemistry of the slag. This work compares the phase chemistry of slags formed during aluminothermic reduction to equilibrium phase chemistries calculated for the Na₂O-SiO₂-Al₂O₃-MnO-CaO system. The slag phase morphology consists of distinct alumina-rich strands (1.5% to 2.1%) embedded within a Na₂O-SiO₂-Al₂O₃-MnO-CaO glass matrix. The alumina-rich strands appear molten, indicating that the processing temperatures were higher than their liquidus temperatures (1537 °C to 1655 °C), as high as 1921 °C and 2053 °C. These findings contribute to sustainable practices in the circular economy through the production of low-carbon ferro-manganese complex alloys. Full article

Background/Objective: Ultraviolet A and B (UVAB) radiation is a major environmental factor that induces DNA damage and upregulates programmed death-ligand 1 (PD-L1) expression in skin cells, thereby contributing to immune evasion and impaired tissue repair. This study evaluated the protective effects of two purified compounds, Cinnamtannin B1 (CTB-1) and Cinnamtannin D1 (CTD-1), as well as cinnamon extract, in UVAB-irradiated human keratinocyte HaCaT cells. Methods: HaCaT cells were exposed to low (20 kJ/m² UVA, 1.3 kJ/m² UVB), medium (30 kJ/m² UVA, 2 kJ/m² UVB), and high (40 kJ/m² UVA, 2.7 kJ/m² UVB) UVAB doses of UVAB radiation. Dose-dependent effects of CTB-1 and CTD-1 (0, 5, 10, 25, and 50 µg/ mL) and cinnamon extract (0, 5, 10, 50, and 100 µg/mL), as well as time-dependent effects (12, 24, and 72 h), were evaluated by measuring PD-L1 expression, cell viability, and DNA damage. Results: CTD-1 was the most effective compound, significantly reducing UVAB-induced PD-L1 expression and DNA double-strand breaks without compromising cell viability. CTB-1 also demonstrated protective effects at specific doses and time points; however, higher concentrations reduced cell viability. Cinnamon extract was protective at low concentrations but cytotoxic at higher doses. Conclusions: CTD-1, CTB-1, and cinnamon extract attenuated UVAB-induced cellular damage in HaCaT cells, with CTD-1 demonstrating the most favorable protective profile. These findings support the potential of cinnamon-derived compounds as therapeutic candidates for preventing UVAB-induced skin damage and immune dysregulation. Full article

Parkinson’s disease and Alzheimer’s disease are currently classified as a major global health burden, sharing a defining pathological hallmark represented by insoluble protein aggregates of α-synuclein (α-syn) and amyloid-β (Aβ), respectively. A defining characteristic of all amyloids is a highly ordered, unbranched filamentous morphology, where individual β-strands align perpendicularly to the filament axis. Despite recent technological advances, direct observation of protein conformational changes and amyloid formation in biological samples remains a challenge as well as the quantification of pathological aggregates in liquid biopsies. This review critically recapitulates the major advances in the application of Raman spectroscopy (RS) and surface-enhanced Raman spectroscopy (SERS) in the investigation of pathological protein aggregates in neurological disorders, with a focus on α-syn and Aβ. We discuss both in vitro structural characterization and the applications to biological and clinical samples, outlining the main challenges for clinical translation, including the need for standardized protocols. Recent achievements in the use of RS and SERS on liquid biopsies and other clinical samples are paving the way for further implementation of Raman-based approaches for the diagnosis of neurodegenerative disorders. Full article

Background/Objectives: Disruption of cellular redox balance and DNA damage response (DDR) signals represents a key driver of cancer development, influencing tumor progression and therapeutic response. Here, we investigated the interplay between DDR-related parameters and oxidative stress in relation to treatment response in patients with multiple myeloma (MM). Methods: Oxidative stress and DDR signals were evaluated in primary cells, including peripheral blood mononuclear cells (PBMCs) and bone marrow plasma cells (BMPCs), collected at diagnosis from MM patients who were subsequently classified as responders (n = 35) or non-responders (n = 41) to melphalan-based therapy. Results: PBMCs and BMPCs from non-responders exhibited a distinct biological profile characterized by lower baseline DNA damage, reduced oxidative stress, increased nucleotide excision repair and double-strand break repair capacity, and reduced apoptotic sensitivity compared with responders (all p < 0.001). In addition, non-responders displayed increased chromatin relaxation. Differential gene expression patterns involving DDR-related pathways further distinguished BMPCs between the two clinical outcome groups. Conclusions: Collectively, these findings indicate that alterations in oxidative stress and DDR signals play a crucial role in determining response to melphalan-based therapy in MM. The identification of these molecular alterations in an easily accessible tissue, such as peripheral blood, underscores their potential clinical relevance and warrants further validation. Full article

Ischemic stroke triggers a severe redox disequilibrium that critically shapes cell survival within the penumbra. Although oxidative DNA damage arises from excessive ROS production, the capacity to repair such lesions is tightly constrained by cellular metabolic status. Growing evidence indicates that astrocytes, key metabolic regulators of the neurovascular unit, modulate neuronal susceptibility to genomic injury through redox buffering, NAD⁺ maintenance, and metabolic support. In the metabolically impaired yet structurally preserved penumbra, astrocytic control of glutathione turnover, mitochondrial function, and lactate shuttling may determine whether oxidative DNA lesions are efficiently repaired or progress toward energetic collapse. Poly(ADP-ribose) polymerase 1 activation following DNA strand breaks couples genomic stress to NAD⁺ depletion and bioenergetic failure, forming a critical interface between redox biology and metabolism. This framework posits that astrocytes preserve genomic integrity not by directly altering DNA repair pathways but by sustaining the energetic capacity required for an effective DNA damage response. Elucidating this astrocyte-centered redox–metabolic axis may reveal therapeutic strategies to stabilize penumbral tissue and improve stroke outcomes. Full article

SIGLECs are well-known receptors that distinguish self from non-self by binding to sialic acid-containing glycoconjugates, thereby regulating normal immune functions. They have also been associated with several diseases, including systemic sclerosis, leukemia, and Alzheimer’s disease. To identify pathogenic regions related to ligand binding in SIGLECs using a novel approach, we employed the in silico Individual Meta Random Forest (InMeRF) program, which predicts disease-related amino acid substitutions. InMeRF predicted a novel three-amino-acid motif (LSI) consisting of highly pathogenic amino acid residues in SIGLEC-7 and other CD33-related SIGLECs. Alanine substitution experiments and point-mutation energy calculations using SIGLEC-7 as a representative model member of the SIGLEC family showed that mutations in the LSI motif altered binding to ganglioside ligands compared with the wild type (WT) and affected structural stability, as reflected by changes in mutation energy. Structural analysis based on the crystal structure of SIGLEC-7 revealed that the LSI motif forms a buried β-strand located beneath the previously identified sialic acid-binding region (Site 2) in CD33-related SIGLEC-7. Taken together, these findings demonstrate the utility of InMeRF for identifying previously unrecognized pathogenic regions and provide new structural and functional insights into the SIGLEC family. Full article

This study examines farmers’ perceptions of how policy support is associated with ecological agriculture adoption and perceived green development outcomes in Xinjiang under China’s Rural Revitalization Strategy. A sequential explanatory mixed-methods design was used, in which the qualitative phase was deliberately connected to the quantitative phase through a shared sampling frame and a construct-aligned interview guide, and the two strands were integrated using a joint display and meta-inferences. In the quantitative phase, survey data from 300 farmers were analyzed using partial least squares structural equation modelling (PLS-SEM) to test the relationships among perceived policy support, ecological agriculture adoption, and green development. In the qualitative phase, semi-structured interviews with 30 participants drawn from the same respondent pool were thematically analyzed to explain, qualify, and contextualize the statistical relationships. The quantitative findings show a strong positive association between perceived policy support and ecological agriculture adoption (β = 0.659, p < 0.001), a strong positive association between ecological agriculture adoption and green development (β = 0.689, p < 0.001), and a smaller but significant direct association between perceived policy support and green development (β = 0.324, p < 0.001). The indirect effect of perceived policy support on green development through ecological agriculture adoption (β = 0.454) indicates partial mediation. The model explains 43.4% of the variance in ecological agriculture adoption and 47.4% of the variance in green development. The integrated joint display shows that technical training, policy clarity, and extension support helped farmers translate policy support into ecological practices, whereas high initial costs, financing constraints, and market uncertainty limited adoption and created uneven outcomes. The integrated findings suggest that policy effectiveness depends not only on the availability of support instruments but also on farmers’ practical capacity, economic security, and confidence in market returns. The study contributes perception-based mixed-method evidence on the policy–adoption–green development nexus in an ecologically vulnerable agricultural region. Full article

Lactic acid bacteria (LAB), as important probiotics, face challenges in applications from bacteriophage infection and the instability of foreign genetic elements. Although Gabija systems and their GajA nuclease components have been characterized in model bacteria, their distribution, biochemical properties, and defensive functions in LAB remain largely unexplored. Here, we provide the first systematic characterization of a naturally occurring Gabija system from Ligilactobacillus salivarius Ren and clarify its distribution among LAB. Approximately 9.3% of LAB strains encode the Gabija system, which exists as a gajA-gajB gene cluster. We found that the Gabija system originated independently in different bacterial lineages. The GajA of L. salivarius Ren (LsGajA) was purified and exhibited non-specific nuclease activity that could efficiently cleave various nucleic acid substrates, including plasmids and linear double-stranded DNA (dsDNA). This activity displayed a temperature-dependent profile, with high activity observed from 45 to 60 °C and at pH 8.0. Mg²⁺ markedly enhanced its degradative nuclease activity, whereas high concentrations of dNTPs inhibited DNA cleavage. LsGajA exhibited substrate-dependent differences in cleavage efficiency, indicating that substrate origin and associated physicochemical features may influence its activity. Additionally, we demonstrate that LsGajA exhibits exceptional stability as a nuclease, retaining activity under a wide range of conditions. The LsGabija system significantly enhanced the ability to reject foreign plasmids and provided strong resistance to the bacteriophage T5 in Escherichia coli. This study provides the first systematic biochemical and functional characterization of the Gabija system in LAB, advancing our understanding of this prokaryotic defense system and highlighting its potential for industrial applications. Full article

Flat-wire windings have been widely used in high-power-density electric vehicle motors because of their high slot fill factor and high efficiency. However, conventional flat-wire conductors usually have relatively large cross-sectional dimensions, which may lead to significant AC winding losses under high-frequency operation due to the combined effects of the rotor magnetic field and the armature-reaction field. To address this issue, this paper proposes a multilayer thin flat-wire continuous-wave winding and its end-winding transposition method. The parallel multilayer thin flat-wire structure effectively suppresses AC losses by reducing the characteristic dimension of each conductor, while the end-winding transposition method reduces or even eliminates circulating-current losses among parallel strands without compromising slot utilization. An analytical calculation method is established to investigate the AC loss characteristics of the multilayer thin flat-wire winding, and the main influencing factors of winding losses are analyzed. To address the circulating-current loss issue, the loss suppression effect of the transposition method is quantitatively evaluated, and an intermittent transposition method with both effective circulating-current suppression and fewer end-winding crossovers is proposed. Finally, the proposed method is validated by finite-element analysis (FEA) and prototype experiments. The results show that the proposed winding can significantly reduce AC losses over a wide speed range, providing a low loss and manufacturable winding design solution for high-power-density electric vehicle traction motors. Full article

Existing timetable optimisation models for urban rail transit predominantly adopt operator-oriented objectives with assumed passenger-related weights. This paper proposes a passenger satisfaction-oriented timetable optimisation framework in which satisfaction weights are empirically derived from confirmatory factor analysis and Cramér’s V analysis of survey data collected from 439 passengers on Nanning Rail Transit Line 1. Seven scheduling-related service attributes are formally expressed as functions of timetable decision variables, establishing a direct linkage between passenger perception and scheduling decisions. A multi-objective model minimises a weighted combination of passenger dissatisfaction, operational cost, and stranded passenger ratio, solved by a Passenger Satisfaction-oriented Adaptive Dispatch Heuristic (PS-ADH) integrating simulated annealing with a passenger flow simulation module. Case study results demonstrate simultaneous improvements of 3.78% in composite objective value, 3.25% in passenger dissatisfaction, and 3.25% in operational cost, with a 27.4% reduction in stranded passengers. The optimised strategy is selected consistently across all ten random initialisations (CV = 0.13%). Sensitivity analysis reveals a structural break at cost weight $\beta =0.4$, beyond which the optimal strategy shifts qualitatively toward cost minimisation at the expense of service quality. The framework provides a transferable methodology for integrating passenger perception data into rail transit scheduling for emerging urban rail systems. Full article