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18 pages, 2148 KB  
Article
Valorization of Treated Olive Mill Wastewater and Olive Pits in Hydroponic Systems for Lettuce Production
by Margarida Oliveira, Renata A. Ferreira, Adelaide Almeida, Annabel Fernandes, Fátima Carvalho and Alexandra Afonso
Water 2026, 18(3), 375; https://doi.org/10.3390/w18030375 (registering DOI) - 1 Feb 2026
Abstract
Significant volumes of wastewater and solid by-products are produced by olive oil industries worldwide, posing serious environmental challenges. This study presents an innovative circular economy and environmental sustainability approach that simultaneously valorizes liquid (olive mill wastewater, OMW) and solid by-products (crushed olive pits) [...] Read more.
Significant volumes of wastewater and solid by-products are produced by olive oil industries worldwide, posing serious environmental challenges. This study presents an innovative circular economy and environmental sustainability approach that simultaneously valorizes liquid (olive mill wastewater, OMW) and solid by-products (crushed olive pits) rom olive oil production through hydroponic lettuce cultivation. The OMW was pretreated and supplemented with nutrients (OMW-N) to create a hydroponic solution for lettuce (Lactuca sativa) cultivation using crushed olive pits as growing substrate. A hydroponic system fed with a nutritive solution was used as a control. Lettuces grown in the OMW-N system achieved a 100% survival rate with no signs of phytotoxicity, although they exhibited a significant reduction in fresh mass (approx. 66%) and size, compared to the control. The sensory analysis revealed no significant differences in consumer acceptance, except for slightly lower color intensity, with 40% of participants explicitly indicating a purchase preference for the OMW-N lettuce, validating its commercial feasibility. Results demonstrated that OMW-N system functioned as a tertiary treatment, achieving additional removal of nutrients. Overall, integrating treated OMW and olive pits into hydroponics is a feasible strategy to convert agro-industrial waste into value-added food products, reducing the environmental footprint of the olive sector. Full article
(This article belongs to the Section Wastewater Treatment and Reuse)
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22 pages, 6646 KB  
Article
Integrating Milk Protein Hydrolysate and Plasma-Activated Water as Alternative Nitrogen Inputs for Growth, Nutrition, and Postharvest Quality of Hydroponic Cos Lettuce Under Low Nutrient Supply
by Aryanis Mutia Zahra, Apiradee Uthairatanakij, Natta Laohakunjit, Pongphen Jitareerat, Nattapon Kaisangsri and Arak Tira-Umphon
Nitrogen 2026, 7(1), 18; https://doi.org/10.3390/nitrogen7010018 (registering DOI) - 1 Feb 2026
Abstract
The application of plasma-activated water and biostimulants offers a sustainable approach to supporting plant growth under reduced-nutrient conditions by supplying bioavailable nitrogen. This study investigated the growth and postharvest performance of hydroponically grown cos lettuce (Lactuca sativa L.) supplied with three Hoagland-based [...] Read more.
The application of plasma-activated water and biostimulants offers a sustainable approach to supporting plant growth under reduced-nutrient conditions by supplying bioavailable nitrogen. This study investigated the growth and postharvest performance of hydroponically grown cos lettuce (Lactuca sativa L.) supplied with three Hoagland-based nutrient treatments: half-strength solution prepared with tap water (HS), half-strength solution with plasma-activated water (HS+PAW), and half-strength solution with plasma-activated water containing 1 mL L−1 milk protein hydrolysate (HS+PAW+MPH). Plants treated with PAW, particularly those in the HS+PAW+MPH, exhibited increases in growth, biomass accumulation, and mineral composition, with reduced nitrate content compared to controls. At harvest, lettuce under HS+PAW+MPH exhibited nearly double fresh yield and enhanced dry matter, protein, lipid, phenolic, and flavonoid profiles as well as increased antioxidant capacity, indicating improved nitrogen utilization and nutritional quality under reduced nutrient input. Postharvest quality was evaluated by packing samples in polypropylene bags and storing them at 10 ± 1 °C and 95–98% relative humidity for 21 days. The HS+PAW+MPH treatment substantially suppressed respiration and production of ethylene, limited weight loss and color change, and better preserved pigments, bioactive compounds, and antioxidant stability compared to HS and HS+PAW, indicating HS+PAW+MPH as a sustainable nutrient management approach for hydroponic systems. Full article
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23 pages, 2079 KB  
Article
Sustainable Intensification of Functional Compounds Recovery from Cocoa Bean Shells Using Flash Extraction
by Carlotta Valle, Silvia Tabasso, Luisa Boffa, Roberto Solarino and Giancarlo Cravotto
Processes 2026, 14(3), 504; https://doi.org/10.3390/pr14030504 (registering DOI) - 1 Feb 2026
Abstract
Cocoa bean shells (CBS) represent a significant by-product of the transformation of cocoa beans, constituting approximately 15% of the total cocoa bean weight. Recently, interest in exploring the potential of these shells as a sustainable source of functional ingredients for use in cosmetics [...] Read more.
Cocoa bean shells (CBS) represent a significant by-product of the transformation of cocoa beans, constituting approximately 15% of the total cocoa bean weight. Recently, interest in exploring the potential of these shells as a sustainable source of functional ingredients for use in cosmetics and nutraceuticals has grown. The present study investigates microwave-assisted subcritical water extraction (MASWE) as a green and fast technique to recover bioactive compounds from CBS. A flash extraction (five minutes) at 170 °C yielded a maximum of 45.78 mg of gallic acid equivalents (GAE) per gram of CBS, which was higher than that obtained using conventional conditions (25.73 mg GAE/g CBS with 50% acetone solution). Additionally, the HPLC profile of the extract from MASWE revealed a significant increase in hydroxybenzoic acids and catechin, compared to the conventional extract. Following the optimization of the extraction process, seven distinct resins were examined to isolate a bioactive-enriched fraction: Sepabeads SP700 was found to be the most effective resin for concentrating such compounds, increasing both methylxanthines and TPC selectivity up to 4.2-fold. This valorization approach, integrating MASWE and downstream optimization, offers an innovative strategy to recover added-value products from CBS in line with green extraction and nutraceutical innovation. Full article
(This article belongs to the Special Issue Resource Utilization of Food Industry Byproducts)
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19 pages, 1053 KB  
Article
Tomato Cultivar and Rootstock Evaluation Under Mg Deficiency: Growth, Mg Uptake, and Leaf Gas Exchange
by Branimir Urlić, Karmen Radovani, Marko Runjić, Maja Veršić Bratinčević, Marijana Popović, Ivana Generalić Mekinić, Zed Rengel and Gvozden Dumičić
Horticulturae 2026, 12(2), 179; https://doi.org/10.3390/horticulturae12020179 (registering DOI) - 31 Jan 2026
Abstract
The importance of magnesium (Mg) is often overlooked in modern crop production. Tomato (Solanum lycopersicum L.) is commonly grafted onto appropriate rootstock to improve the nutrient uptake, which may have a negative effect on the tomato Mg leaf concentration and possibly influence [...] Read more.
The importance of magnesium (Mg) is often overlooked in modern crop production. Tomato (Solanum lycopersicum L.) is commonly grafted onto appropriate rootstock to improve the nutrient uptake, which may have a negative effect on the tomato Mg leaf concentration and possibly influence the carbohydrate partitioning required for optimal crop yield and quality. The aim of this study was to screen tomato cultivars and rootstocks under Mg deficiency using two experiments. The first experiment included a panel of 14 tomato cultivars and 10 rootstocks grown with 1 or 0.1 mM Mg in nutrient solution. The second experiment consisted of four cultivars either self-grafted or grafted onto four rootstocks chosen from the first experiment. In both experiments, most of the plants grown under low-Mg conditions, on average, had a higher biomass production. The magnesium concentrations in the leaves and stems (but not in the roots) of both cultivars and rootstocks, non-grafted or grafted, were significantly higher under optimal Mg supply. Regarding the Mg content, the differences between the Mg supplies were up to three-fold for cultivars, up to two-fold for the rootstocks, and up to five-fold for the combinations of grafted plants. Our results showed that genotypic differences between used tomato cultivars and rootstocks in response to Mg can be observed at early developmental stages and can possibly serve as a tool in screening programs, but further research is needed to assess their relationship with long-term cultivation. Full article
(This article belongs to the Special Issue Physiology of Vegetables Under Biotic/Abiotic Stress Conditions)
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13 pages, 4701 KB  
Article
Physiological and Biochemical Aspects in Physalis angulata L. Accessions Cultivated Under Water Deficit
by Robson de Jesus Santos, Marilza Neves do Nascimento, Romeu da Silva Leite, Gabriela Torres-Silva, Uasley Caldas De Oliveira, Aritana Alves da Silva, Maryelle Vanilla de Abreu Cerqueira and Gabrielly dos Santos Lima Oliveira
Horticulturae 2026, 12(2), 172; https://doi.org/10.3390/horticulturae12020172 - 30 Jan 2026
Abstract
Drought is the primary stress factor in semiarid environments. Consequently, selecting plant genetic resources capable of tolerating temporary periods of water scarcity, such as Physalis angulata, becomes essential. This study aimed to identify P. angulata accessions with potential for use under water [...] Read more.
Drought is the primary stress factor in semiarid environments. Consequently, selecting plant genetic resources capable of tolerating temporary periods of water scarcity, such as Physalis angulata, becomes essential. This study aimed to identify P. angulata accessions with potential for use under water deficit conditions by evaluating plant water status and physiological and biochemical responses. Five accessions, including two from Bahia (BA1 and BA2), Pará-PA, Rio de Janeiro-RJ, and Piauí-PI, were grown under well-watered and water deficit conditions. Relative water content, gas exchange parameters, and organic solute accumulation were assessed. All accessions exhibited changes in plant water status and reductions in CO2 assimilation, stomatal conductance, and leaf transpiration under water deficit. The accumulation of compatible solutes varied among accessions, with notable contrasts between Bahia accession 2 and Pará accession, particularly for total soluble sugars and reducing sugars. These findings highlight the complexity of the species and the distinct mechanisms underlying its response to limited water availability. Overall, gas exchange was the trait most sensitive to water restriction, followed by alterations in biochemical attributes. Therefore, the Physalis angulata accessions from Bahia accession 2 and Pará accession show potential for use under water-deficit conditions and could provide valuable insights, particularly through transcriptome analysis. Full article
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23 pages, 1497 KB  
Systematic Review
Home-Grown Indigenous Vegetables and Risk Mitigation Strategies for Enhancing Food and Nutrition Security Among Small-Scale Farming Households: A Systematic Review
by Nkosingimele Ndwandwe, Bonguyise Mzwandile Dumisa, Phumza Tabalaza, Neliswa Ntshangase, Melusi Sibanda and Nolwazi Zanele Khumalo
Sustainability 2026, 18(3), 1176; https://doi.org/10.3390/su18031176 - 23 Jan 2026
Viewed by 147
Abstract
Achieving food and nutrition security remains a significant challenge for small-scale farmers in Sub-Saharan Africa (SSA). However, indigenous vegetables offer a promising solution to this challenge. This systematic review used four databases and retrieved 38 studies published over the past 20 years for [...] Read more.
Achieving food and nutrition security remains a significant challenge for small-scale farmers in Sub-Saharan Africa (SSA). However, indigenous vegetables offer a promising solution to this challenge. This systematic review used four databases and retrieved 38 studies published over the past 20 years for synthesis. These studies highlight the growing importance of indigenous vegetables grown in home gardens as a sustainable solution to improve livelihoods and dietary diversity. Indigenous vegetables are well-suited to local conditions, nutritionally rich, and were associated with improved household food availability and income. However, farmers face various risks, including environmental, technological, economic, institutional, and social risks, which threaten their production. To overcome risks, farmers adopt strategies such as training, cooperatives, improved storage, and better seed varieties. Indigenous vegetable cultivation also empowers women and marginalised groups who play key roles in home gardening. Despite their benefits, indigenous vegetables remain overlooked in mainstream markets and policies. The review recommends that policymakers and stakeholders must provide support to promote indigenous vegetables through training and market integration, helping farmers commercialise their produce, while simultaneously enhancing food and nutrition security. Further research is needed to explore the profitability of indigenous vegetable production, analyse supply value chains, and investigate processing and manufacturing opportunities to support their market potential and sustainability. Full article
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18 pages, 2924 KB  
Article
Path Planning for a Cartesian Apple Harvesting Robot Using the Improved Grey Wolf Optimizer
by Dachen Wang, Huiping Jin, Chun Lu, Xuanbo Wu, Qing Chen, Lei Zhou, Xuesong Jiang and Hongping Zhou
Agronomy 2026, 16(2), 272; https://doi.org/10.3390/agronomy16020272 - 22 Jan 2026
Viewed by 122
Abstract
As a high-value fruit crop grown worldwide, apples require efficient harvesting solutions to maintain a stable supply. Intelligent harvesting robots represent a promising approach to address labour shortages. This study introduced a Cartesian robot integrated with a continuous-picking end-effector, providing a cost-effective and [...] Read more.
As a high-value fruit crop grown worldwide, apples require efficient harvesting solutions to maintain a stable supply. Intelligent harvesting robots represent a promising approach to address labour shortages. This study introduced a Cartesian robot integrated with a continuous-picking end-effector, providing a cost-effective and mechanically simpler alternative to complex articulated arms. The system employed a hand–eye calibration model to enhance positioning accuracy. To overcome the inefficiencies resulting from disordered harvesting sequences and excessive motion trajectories, the harvesting process was treated as a travelling salesman problem (TSP). The conventional fixed-plane return trajectory of Cartesian robots was enhanced using a three-dimensional continuous picking path strategy based on a fixed retraction distance (H). The value of H was determined through mechanical characterization of the apple stem’s brittle fracture, which eliminated redundant horizontal displacements and improved operational efficiency. Furthermore, an improved grey wolf optimizer (IGWO) was proposed for multi-fruit path planning. Simulations demonstrated that the IGWO achieved shorter path lengths compared to conventional algorithms. Laboratory experiments validated that the system successfully achieved vision-based localization and fruit harvesting through optimal path planning, with a fruit picking success rate of 89%. The proposed methodology provides a practical framework for automated continuous harvesting systems. Full article
(This article belongs to the Section Precision and Digital Agriculture)
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32 pages, 8317 KB  
Article
Research Progress and Frontier Trends in Generative AI in Architectural Design
by Yingli Yang, Yanxi Li, Xuefei Bai, Wei Zhang and Siyu Chen
Buildings 2026, 16(2), 388; https://doi.org/10.3390/buildings16020388 - 17 Jan 2026
Viewed by 280
Abstract
In recent years, with the rapid advancement of science and technology, generative artificial intelligence has increasingly entered the public eye. Primarily through intelligent algorithms that simulate human logic and integrate vast amounts of network data, it provides designers with solutions that transcend traditional [...] Read more.
In recent years, with the rapid advancement of science and technology, generative artificial intelligence has increasingly entered the public eye. Primarily through intelligent algorithms that simulate human logic and integrate vast amounts of network data, it provides designers with solutions that transcend traditional thinking, enhancing both design efficiency and quality. Compared to traditional design methods reliant on human experience, generative design possesses robust data processing capabilities and the ability to refine design proposals, significantly reducing preliminary design time. This study employs the CiteSpace visualization tool to systematically organize and conduct knowledge map analysis of research literature related to generative AI in architectural design within the Web of Science database from 2005 to 2025. Findings reveal the following: (1) International research exhibits a trend toward interdisciplinary convergence. In recent years, research in this field has grown rapidly across nations, with continuously increasing academic influence; (2) Research primarily focuses on technological applications within architectural design, aiming to drive innovation and development by providing superior, more efficient technical support; (3) Generative AI in architectural design has emerged as a prominent international research focus, reflecting a shift from isolated design to industry-wide integration; (4) Generative AI has become a core global architectural design topic, with future research advancing toward full-process intelligent collaboration. High-quality knowledge graphs tailored for the architecture industry should be constructed to overcome data silos. Concurrently, a multidimensional evaluation system for generative quality must be established to deepen the symbiotic design paradigm of human–machine collaboration. This significantly enhances efficiency while reducing the iterative nature of traditional methods. This study aims to provide empirical support for theoretical and practical advancements, offering crucial references for practitioners to identify business opportunities and policymakers to optimize relevant strategies. Full article
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26 pages, 4292 KB  
Article
Mechanism of Long-Term Corrosion Protection for Silicone Epoxy Coatings Reinforced by BN-PDA-CeO2 Ternary Composites in Harsh Environments
by Xianlian Mu, Tao Jin, Pengfei Xie, Rongcao Yu, Bin Li and Xin Yuan
Nanomaterials 2026, 16(2), 121; https://doi.org/10.3390/nano16020121 - 16 Jan 2026
Viewed by 230
Abstract
Corrosion in harsh environments causes global economic losses exceeding 3 trillion US dollars annually. Traditional silicone epoxy (SE) coatings are prone to failure due to insufficient physical barrier properties and lack of active protection. In this study, cerium dioxide (CeO2) was [...] Read more.
Corrosion in harsh environments causes global economic losses exceeding 3 trillion US dollars annually. Traditional silicone epoxy (SE) coatings are prone to failure due to insufficient physical barrier properties and lack of active protection. In this study, cerium dioxide (CeO2) was in situ grown on the surface of hexagonal boron nitride (h-BN) mediated by polydopamine (PDA) to prepare BN-PDA-CeO2 ternary nanocomposites, which were then incorporated into SE coatings to construct a multi-scale synergistic corrosion protection system. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and transmission electron microscopy (TEM) confirmed the successful preparation of the composites, where PDA inhibited the agglomeration of h-BN and CeO2 was uniformly loaded. Electrochemical tests showed that the corrosion inhibition efficiency of the extract of this composite for 2024 aluminum alloy reached 99.96%. After immersing the composite coating in 3.5 wt% NaCl solution for 120 days, the coating resistance (Rc) and charge transfer resistance (Rct) reached 8.5 × 109 Ω·cm2 and 1.2 × 1010 Ω·cm2, respectively, which were much higher than those of pure SE coatings and coatings filled with single/binary fillers. Density functional theory (DFT) calculations revealed the synergistic mechanisms: PDA enhanced interfacial dispersion (adsorption energy of −0.58 eV), CeO2 captured Cl (adsorption energy of −4.22 eV), and Ce3+ formed a passive film. This study provides key technical and theoretical support for the design of long-term corrosion protection coatings in harsh environments such as marine and petrochemical industries. Full article
(This article belongs to the Special Issue Research and Applications of Anti-Corrosion Nanocoatings)
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21 pages, 7908 KB  
Article
Bi-Level Decision-Making for Commercial Charging Stations in Demand Response Considering Nonlinear User Satisfaction
by Weiqing Sun, En Xie and Wenwei Yang
Sustainability 2026, 18(2), 907; https://doi.org/10.3390/su18020907 - 15 Jan 2026
Viewed by 163
Abstract
With the widespread adoption of electric vehicles, commercial charging stations (CCS) have grown rapidly as a core component of charging infrastructure. Due to the concentrated and high-power charging load characteristics of CCS, a ‘peak on peak’ phenomenon can occur in the power distribution [...] Read more.
With the widespread adoption of electric vehicles, commercial charging stations (CCS) have grown rapidly as a core component of charging infrastructure. Due to the concentrated and high-power charging load characteristics of CCS, a ‘peak on peak’ phenomenon can occur in the power distribution network. Demand response (DR) serves as an important and flexible regulation tool for power systems, offering a new approach to addressing this issue. However, when CCS participates in DR, it faces a dual dilemma between operational revenue and user satisfaction. To address this, this paper proposes a bi-level, multi-objective framework that co-optimizes station profit and nonlinear user satisfaction. An asymmetric sigmoid mapping is used to capture threshold effects and diminishing marginal utility. Uncertainty in users’ charging behaviors is evaluated using a Monte Carlo scenario simulation together with chance constraints enforced at a 0.95 confidence level. The model is solved using the fast non-dominated sorting genetic algorithm, NSGA-II, and the compromise optimal solution is identified via the entropy-weighted Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS). Case studies show robust peak shaving with a 6.6 percent reduction in the daily maximum load, high satisfaction with a mean of around 0.96, and higher revenue with an improvement of about 12.4 percent over the baseline. Full article
(This article belongs to the Section Energy Sustainability)
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15 pages, 766 KB  
Article
Analyzing the Effect of Arbuscular Mycorrhizal Fungi and Plant Growth-Promoting Bacteria Inoculation over the Growth of Tomatoes in a Martian Regolith Analog: Perspectives for Martian Agriculture
by Daniel Fernando Cortez Acosta, Víctor Olalde Portugal, Rufino Lozano Santacruz and Sergio Valle Cervantes
Microorganisms 2026, 14(1), 200; https://doi.org/10.3390/microorganisms14010200 - 15 Jan 2026
Viewed by 218
Abstract
For future Mars colonization, crop production will be a challenge due to the chemical composition of the Martian Regolith, which contains perchlorates and heavy metals. This research was conducted to determine if the use of Arbuscular Mycorrhizal Fungi (AMF), Plant Growth-Promoting Bacteria (PGPB), [...] Read more.
For future Mars colonization, crop production will be a challenge due to the chemical composition of the Martian Regolith, which contains perchlorates and heavy metals. This research was conducted to determine if the use of Arbuscular Mycorrhizal Fungi (AMF), Plant Growth-Promoting Bacteria (PGPB), and fertilization have a positive effect on tomato growth in a Martian Regolith Analog. The analog contains 52.54% SiO2, 1.81% TiO2, 17.66% Al2O3, 9.46% Fe2O3, 0.145% MnO, 3.43% MgO, 7.09% CaO, 3.95% Na2O, 1.96% K2O, and 0.55% P2O5. Two hundred and forty tomato plants were grown for 45 days. One hundred and twenty tomato plants grown over perchlorate-polluted analog (1% m/m) died in less than 2 weeks, while 120 tomato plants grown in a non-polluted analog survived. Forty-eight plants supplemented with Long–Ashton solution increased their shoot length 100% more than the control plants and the plants inoculated with the commercial AMF formulation TM-73MR and PBB; the latter showed 25% mycorrhizal colonization. There was no significant difference between the growth parameters of inoculated plants and non-inoculated plants. However, there was a significant difference compared to the plants supplemented with Long–Ashton solution. The perchlorate is toxic to tomato plants, and the metal content of the analog was not a limiting factor for tomato growth or AMF colonization. Full article
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27 pages, 7708 KB  
Article
Effects of Substrate-Based Root Restriction on Tomato Growth, Fruit Quality, Yield, and Microbial Communities in a Simplified Automatic Soilless Cultivation System
by Yecheng Jin, Siqi Xia, Haili Zhang, Lingyu Wang, Ying Zhou, Jie Zhou, Xiaojian Xia, Nianqiao Shen and Zhenyu Qi
Agronomy 2026, 16(2), 212; https://doi.org/10.3390/agronomy16020212 - 15 Jan 2026
Viewed by 182
Abstract
Root restriction is an agronomic technique that influences plant morphology, physiology, and productivity. This study investigates the effects of root restriction on tomato growth, fruit quality, yield, and rhizosphere microbial communities using three distinct substrates: sand, soil, and peanut shell substrate (PSS), within [...] Read more.
Root restriction is an agronomic technique that influences plant morphology, physiology, and productivity. This study investigates the effects of root restriction on tomato growth, fruit quality, yield, and rhizosphere microbial communities using three distinct substrates: sand, soil, and peanut shell substrate (PSS), within a Simplified Automatic Soilless Culture System (SAS). Results demonstrated that root restriction at 8 cm height significantly enhanced fruit quality indicators: soluble sugar content increased by 69.01% (sand), 53.84% (soil), and 37.67% (PSS); soluble protein increased by 77.23%, 48.14%, and 66.51%; and lycopene increased by 100.03%, 62.33%, and 74.59%, respectively, compared to the 24 cm baseline. However, single-plant yield declined by 28.30% (sand), 64.28% (soil), and 22.06% (PSS). TOPSIS analysis (Technique for Order Preference by Similarity to Ideal Solution) identified PSS at 8 cm as the optimal combination for balancing quality and yield (Cj = 0.631). Microbial amplicon sequencing revealed higher rhizosphere microbial diversity in tomatoes grown in soil and peanut shell substrate compared to sand. These three types of growing media (soil, sand, and peanut shell substrate) establish the rhizosphere of bacterial and fungal communities by selecting specific microbial taxa. Changes in container height drive the reduction–oxidation functional divergence of bacterial communities, affecting the connectivity and complexity of microbial networks. Full article
(This article belongs to the Section Horticultural and Floricultural Crops)
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17 pages, 3151 KB  
Article
Exploring the Effects of Diluted Plasma-Activated Water (PAW) on Various Sprout Crops and Its Role in Autophagy Regulation
by Injung Song, Suji Hong, Yoon Ju Na, Seo Yeon Jang, Ji Yeong Jung, Young Koung Lee and Sung Un Huh
Agronomy 2026, 16(2), 207; https://doi.org/10.3390/agronomy16020207 - 15 Jan 2026
Viewed by 258
Abstract
Plasma-activated water (PAW) has gained attention across agricultural, medical, cosmetic, and sterilization fields due to its production of reactive oxygen and nitrogen species (ROS and RNS). Although PAW has been primarily explored for seed germination and sterilization in agriculture, its role as a [...] Read more.
Plasma-activated water (PAW) has gained attention across agricultural, medical, cosmetic, and sterilization fields due to its production of reactive oxygen and nitrogen species (ROS and RNS). Although PAW has been primarily explored for seed germination and sterilization in agriculture, its role as a nutrient source and physiological regulator remains less understood. In this study, PAW generated by a surface dielectric barrier discharge (SDBD) system contained approximately 1000 ppm nitrate (NO3) and was designated as PAW1000. Diluted PAW solutions were applied to sprout crops—wheat (Triticum aestivum), barley (Hordeum vulgare), radish (Raphanus sativus), and broccoli (Brassica oleracea var. italica)—grown under hydroponic and soil-based conditions. PAW100 and PAW200 treatments enhanced growth, increasing fresh biomass by up to 26%, shoot length by 22%, and root length by 18%, depending on the species. In silico analysis identified nitrogen-responsive transcripts among several autophagy-related genes. Consistent with this, fluorescence microscopy of Arabidopsis thaliana GFP-StATG8 lines revealed increased autophagosome formation following PAW treatment. The growth-promoting effect of PAW was diminished in atg4 mutants, indicating that autophagy contributes to plant responses to PAW-derived ROS and RNS. Together, these findings demonstrate that diluted PAW generated by SDBD enhances biomass accumulation in sprout crops, and that autophagy plays a regulatory role in mediating PAW-induced physiological responses. Full article
(This article belongs to the Topic Applications of Biotechnology in Food and Agriculture)
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26 pages, 2231 KB  
Review
Microneedle Technologies for Drug Delivery: Innovations, Applications, and Commercial Challenges
by Kranthi Gattu, Deepika Godugu, Harsha Jain, Krishna Jadhav, Hyunah Cho and Satish Rojekar
Micromachines 2026, 17(1), 102; https://doi.org/10.3390/mi17010102 - 13 Jan 2026
Viewed by 611
Abstract
Microneedle (MN) technologies have emerged as a groundbreaking platform for transdermal and intradermal drug delivery, offering a minimally invasive alternative to oral and parenteral routes. Unlike passive transdermal systems, MNs allow the permeation of hydrophilic macromolecules, such as peptides, proteins, and vaccines, by [...] Read more.
Microneedle (MN) technologies have emerged as a groundbreaking platform for transdermal and intradermal drug delivery, offering a minimally invasive alternative to oral and parenteral routes. Unlike passive transdermal systems, MNs allow the permeation of hydrophilic macromolecules, such as peptides, proteins, and vaccines, by penetrating the stratum corneum barrier without causing pain or tissue damage, unlike hypodermic needles. Recent advances in materials science, microfabrication, and biomedical engineering have enabled the development of various MN types, including solid, coated, dissolving, hollow, hydrogel-forming, and hybrid designs. Each type has unique mechanisms, fabrication techniques, and pharmacokinetic profiles, providing customized solutions for a range of therapeutic applications. The integration of 3D printing technologies and stimulus-responsive polymers into MN systems has enabled patches that combine drug delivery with real-time physiological sensing. Over the years, MN applications have grown beyond vaccines to include the delivery of insulin, anticancer agents, contraceptives, and various cosmeceutical ingredients, highlighting the versatility of this platform. Despite this progress, broader clinical and commercial adoption is still limited by issues such as scalable and reliable manufacturing, patient acceptance, and meeting regulatory expectations. Overcoming these barriers will require coordinated efforts across engineering, clinical research, and regulatory science. This review thoroughly summarizes MN technologies, beginning with their classification and drug-delivery mechanisms, and then explores innovations, therapeutic uses, and translational challenges. It concludes with a critical analysis of clinical case studies and a future outlook for global healthcare. By comparing technological progress with regulatory and commercial hurdles, this article highlights the opportunities and limitations of MN systems as a next-generation drug-delivery platform. Full article
(This article belongs to the Special Issue Breaking Barriers: Microneedles in Therapeutics and Diagnostics)
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10 pages, 233 KB  
Proceeding Paper
Artificial Intelligence in Satellite Network Defense: Architectures, Threats, and Security Protocols
by Rumen Doynov, Maksim Sharabov, Georgi Tsochev and Samiha Ayed
Eng. Proc. 2026, 121(1), 7; https://doi.org/10.3390/engproc2025121007 - 13 Jan 2026
Viewed by 332
Abstract
This paper examines the application of Artificial Intelligence (AI) to protect satellite communication networks, focusing on the identification and prevention of cyber threats. With the rapid development of the commercial space sector, the importance of effective cyber defense has grown due to the [...] Read more.
This paper examines the application of Artificial Intelligence (AI) to protect satellite communication networks, focusing on the identification and prevention of cyber threats. With the rapid development of the commercial space sector, the importance of effective cyber defense has grown due to the increasing dependence of global infrastructure on satellite technologies. The study applies a structured comparative analysis of AI methods across three main satellite architectures: geostationary (GEO), low Earth orbit (LEO), and hybrid systems. The methodology is based on guiding research question and evaluates representative AI algorithms in the context of specific threat scenarios, including jamming, spoofing, DDoS attacks, and signal interception. Real-world cases such as the KA-SAT AcidRain attack and reported Starlink jamming in Ukraine, as well as experimental demonstrations of RL-based anti-jamming and GNN/DQN routing, are used to provide evidence of practical applicability. The results highlight both the potential and limitations of AI solutions, showing measurable improvements in detection accuracy, throughput, latency reduction, and resilience under interference. Architectural approaches for integrating AI into satellite security are presented, and their effectiveness, trade-offs, and deployment feasibility are discussed. Full article
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