Search Results (350)

The multiscale interactive system composed of wind, leaves, and droplets serves as a critical dynamic unit in precision orchard spraying. Its coupling mechanisms fundamentally influence pesticide transport pathways, deposition patterns, and drift behavior within crop canopies, forming the foundational basis for achieving intelligent and site-specific spraying operations. This review systematically examines the synergistic dynamics across three hierarchical scales: Droplet–leaf surface wetting and adhesion at the microscale; leaf cluster motion responses at the mesoscale; and the modulation of airflow and spray plume diffusion by canopy architecture at the macroscale. Key variables affecting spray performance—such as wind speed and turbulence structure, leaf biomechanical properties, droplet size and electrostatic characteristics, and spatial canopy heterogeneity—are identified and analyzed. Furthermore, current advances in multiscale modeling approaches and their corresponding experimental validation techniques are critically evaluated, along with their practical boundaries of applicability. Results indicate that while substantial progress has been made at individual scales, significant bottlenecks remain in the integration of cross-scale models, real-time acquisition of critical parameters, and the establishment of high-fidelity experimental platforms. Future research should prioritize the development of unified coupling frameworks, the integration of physics-based and data-driven modeling strategies, and the deployment of multimodal sensing technologies for real-time intelligent spray decision-making. These efforts are expected to provide both theoretical foundations and technological support for advancing precision and intelligent orchard spraying systems. Full article

This study characterized collagen remodeling in an electron-beam-sterilized porcine acellular dermal matrix (E-PADM) by evaluating host response kinetics during wound healing. E-PADM (n = 6 lots/time point) was implanted in an abdominal wall bridging defect in nonhuman primates (N = 24). Histological, immunohistochemical, and biochemical assessments were conducted. Pro-inflammatory tissue cytokines peaked 1 month post-implantation and subsided to baseline by 6 months. E-PADM-specific serum immunoglobulin G antibodies increased by 213-fold from baseline at 1 month, then decreased to <10-fold by 6–9 months. The mean percentage tissue area staining positively for matrix metalloproteinase-1 plateaued at 3 months (40.3 ± 16.9%), then subsided by 6 months (16.3 ± 11.1%); tissue inhibitor matrix metalloproteinase-1 content plateaued at 1 month (39.0 ± 14.3%), then subsided by 9 months (13.0 ± 8.8%). Mean E-PADM thickness (1.7 ± 0.2 mm pre-implant) increased at 3 months (2.9 ± 1.5 mm), then decreased by 9 months (1.9 ± 1.1; equivalent to pre-implant). Histology demonstrated mild inflammation between 1–3 months, then a peak in host tissue deposition, with ≈75%–100% E-PADM collagen turnover, and fibroblast infiltration and neovascularization between 3–6 months. Picrosirius red staining revealed that mature E-PADM collagen was replaced by host-associated neo-collagen by 6 months. E-PADM implantation induced wound healing, which drove dermal E-PADM collagen remodeling to native, functional fascia-like tissue at the implant site. Full article

The remarkable advantages of zinc anodes render aqueous zinc-ion batteries (ZIBs) a highly promising energy storage solution. Nevertheless, the uncontrolled growth of zinc dendrites and side reactions pose significant obstacles to the practical application of ZIBs. To address these issues, a straightforward strategy has been proposed, involving the addition of a minute quantity of AgNO₃ to the electrolyte to stabilize zinc anodes. This additive spontaneously forms a hierarchically porous Ag interphase on the zinc anodes, which is characterized by its zinc-affinitive nature. The interphase offers abundant zinc nucleation sites and accommodation space, leading to uniform zinc plating/stripping and enhanced kinetics of zinc deposition/dissolution. Moreover, the chemically inert Ag interphase effectively curtails side reactions by isolating water molecules. Consequently, the incorporation of AgNO₃ enables zinc anodes to undergo cycling for extended periods, such as over 4000 h at a current density of 0.5 mA/cm² with a capacity of 0.5 mAh/cm², and for 450 h at 2 mA/cm² with a capacity of 2 mAh/cm². Full zinc-ion cells equipped with this additive not only demonstrate increased specific capacities but also exhibit significantly improved cycle stability. This research presents a cost-effective and practical approach for the development of reliable zinc anodes for ZIBs. Full article

Glycated hemoglobin (HbA1c) is an important biomarker applied for the diagnosis, evaluation, and management of diabetes; therefore, its accurate determination is crucial. In this study, an innovative nanoplatform was developed, integrating carbon nanotubes (CNTs) with enhanced hydrophilicity achieved through cyclodextrin (CD) functionalization, and combined with gold nanoparticles (AuNPs) electrochemically deposited onto a screen-printed carbon electrode. The nanomaterials significantly improved the analytical performance of the sensor due to their increased surface area and high electrical conductivity. This nanoplatform was employed as a substrate for the covalent attachment of thiolated ferrocene-labeled HbA1c specific aptamer through Au-S binding. The electrochemical signal of ferrocene was covered by a stronger oxidation peak of Fe²⁺ from the HbA1c structure, leading to the elaboration of a nanostructured aptasensor capable of the direct detection of HbA1c. The electrochemical aptasensor presented a very wide linear range (0.688–11.5%), an acceptable limit of detection (0.098%), and good selectivity and stability, being successfully applied on real samples. This miniaturized, simple, easy-to-use, and fast-responding aptasensor, requiring only a small sample volume, can be considered as a promising candidate for the efficient on-site determination of HbA1c. Full article

With ongoing global warming, glacier lake outburst floods (GLOFs) and associated debris flows pose increasing threats to downstream communities and infrastructure. Glacial lakes differ in their triggering factors and breach mechanisms, necessitating event-specific analysis. This study investigates the GLOF risk of Jiongpuco Lake, located in the southeastern part of the Tibetan Plateau, using an integrated approach combining remote sensing, field surveys, and numerical modeling. Results show that the lake has expanded significantly—from 2.08 km² in 1990 to 5.43 km² in 2021—with the most rapid increase observed between 2015 and 2016. InSAR data and optical imagery indicate that surrounding moraine deposits remain generally stable. However, ice avalanches from the glacier terminus are identified as the primary trigger for lake outburst via wave-induced overtopping. Mechanical and geomorphological analyses suggest that the moraine dam is resistant to downcutting erosion, reinforcing overtopping as the dominant failure mode. To assess potential impacts, three numerical simulation scenarios were conducted based on different avalanche volumes. Under the extreme scenario involving a 5-million m³ ice avalanche, the modeled peak discharge at the dam site reaches approximately 19,000 m³/s. Despite the high flood magnitude, the broad and gently sloped downstream terrain facilitates rapid attenuation of flood peaks, resulting in limited impact on downstream settlements. These findings offer critical insights for GLOF hazard assessment, disaster preparedness, and risk mitigation under a changing climate. Full article

This study investigates the impact of nonlinear constitutive models on one-dimensional seismic site response analysis (SRA) for soft, reclaimed soil deposits in Saemangeum, South Korea. Two widely used models, MKZ and GQ/H, were applied to three representative soil profiles using the DEEPSOIL program. Ground motions were scaled to bedrock peak ground accelerations (PGAs) corresponding to annual return periods (ARPs) of 1000, 2400, and 4800 years. Seismic response metrics include the ratio of GQ/H to MKZ shear strain, effective PGA (EPGA), and short- and long-term amplification factors (F_a and F_v). The results highlight the critical role of the site-to-motion period ratio (T_g/T_m) in controlling seismic behavior. Compared to the MKZ, the GQ/H model, which features strength correction and improved stiffness retention, predicts lower shear strains and higher surface spectral accelerations, particularly under strong shaking and shallow conditions. Model differences are most pronounced at low T_g/T_m values, where MKZ tends to underestimate amplification and overestimate strain due to its limited ability to reflect site-specific shear strength. Relative to code-based amplification factors, the GQ/H model yields lower short-term estimates, reflecting the disparity between stiff inland reference sites and the soft reclaimed conditions at Saemangeum. These findings emphasize the need for strength-calibrated constitutive models to improve the accuracy of site-specific seismic hazard assessments. Full article

Bone is a preferred site for disseminated tumor cells, yet the molecular mechanisms that prepare the skeletal microenvironment for metastatic colonization are only beginning to be understood. At the heart of this process are extracellular vesicles (EVs), nano-sized, lipid-encapsulated particles secreted by cancer cells and stromal components. This review consolidates current findings that position EVs as key architects of the bone-metastatic niche. We detail the biogenesis of EVs and their organotropic distribution, focusing on how integrin patterns and bone-specific ligands guide vesicle homing to mineralized tissues. We then outline the sequential establishment of the pre-metastatic niche, driven by EV-mediated processes including fibronectin deposition, stromal cell reprogramming, angiogenesis, neurogenesis, metabolic reconfiguration, and immune modulation, specifically, the expansion of myeloid-derived suppressor cells and impaired lymphocyte function. Within the bone microenvironment, tumor-derived EVs carrying microRNAs and proteins shift the balance toward osteoclastogenesis, inhibit osteoblast differentiation, and disrupt osteocyte signaling. These alterations promote osteolytic destruction or aberrant bone formation depending on tumor type. We also highlight cutting-edge imaging modalities and single-EV omics technologies that resolve EV heterogeneity and identify potential biomarkers detectable in plasma and urine. Finally, we explore therapeutic approaches targeting EVs, such as inhibition of nSMase2 or Rab27A, extracorporeal EV clearance, and delivery of engineered, bone-targeted vesicles, while addressing translational challenges and regulatory considerations. This review offers a roadmap for leveraging EV biology in predicting, preventing, and treating skeletal metastases by integrating advances across basic biology, bioengineering, and translational science. Full article

An electrochemical immunosensor for the quantification of prostate-specific antigens (PSAs) using silver nanocrystals (AgNCs) is reported. The silver nanocrystals were synthesized using a conventional citrate reduction protocol. The silver nanocrystals were characterized using scanning electron microscopy (SEM) and field effect scanning electron microscopy (FESEM), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), Fourier-transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, and small-angle X-ray scattering (SAXS). The proposed immunosensor was fabricated on a glassy carbon electrode (GCE), sequentially, by drop-coating AgNCs, the electro-deposition of EDC-NHS, the immobilization of anti-PSA antibody (Ab), and dropping of bovine serum albumin (BSA) to prevent non-specific binding sites. Each stage of the fabrication process was characterized by cyclic voltammetry (CV). Using square wave voltammetry (SWV), the proposed immunosensor displayed high sensitivity in detecting PSA over a concentration range of 1 to 10 ng/mL with a detection limit of 1.14 ng/mL and R² of 0.99%. The immunosensor was selective in the presence of interfering substances like glucose, urea, L-cysteine, and alpha-methylacyl-CoA racemase (AMACR) and it showed good stability and repeatability. These results compare favourably with some previously reported results on similar or related technologies for PSA detection. Full article

Parabolic trough collector (PTC) systems, often deployed in arid regions, are vulnerable to dust accumulation (soiling), which reduces mirror reflectivity and energy output. This study presents a physically based soiling forecast algorithm (SFA) designed to estimate soiling levels. The model was calibrated and validated using three meteorological data sources—numerical forecasts (YR), METAR observations, and on-site measurements—from a PTC facility in Limassol, Cyprus. Field campaigns covered dry, rainy, and red-rain conditions. The model demonstrated robust performance, particularly under dry summer conditions, with normalized root mean square errors (NRMSE) below 1%. Sedimentation emerged as the dominant soiling mechanism, while the contributions of impaction and Brownian motion varied according to site-specific environmental conditions. Under dry deposition conditions, the reflectivity change rate during spring and autumn was approximately twice that of summer, indicating a need for more frequent cleaning during transitional seasons. A red-rain event resulted in a pronounced drop in reflectivity, showcasing the model’s ability to capture abrupt soiling dynamics associated with extreme weather episodes. The proposed SFA offers a practical, adaptable tool for reducing soiling-related losses and supporting seasonally adjusted maintenance strategies for solar thermal systems. Full article

The present study is part of the Horizon Europe-START project, which aims to recover tetrahedrite-group minerals present in mine dumps to be used as raw materials for the manufacture of thermoelectric devices. The aim of this work is to identify the mining waste facilities selected in Spain for the recovery of tetrahedrite and to outline the mineral processing operations performed on samples from each site to separate and concentrate this mineral. Ore deposits across Spain were selected based on the potential presence of tetrahedrite in their mining waste. A total of five deposits have been sampled, at which subsequent mineral separation and concentration tests have been conducted. A separation flowsheet is proposed in order to extract a high-purity tetrahedrite concentrate. Experimental results indicate two distinct options for separation approaches, depending on a key parameter that proves decisive in the processing of this mineral, which is whether the mineral paragenesis includes siderite. This study has demonstrated the technical feasibility of concentrating minerals of the tetrahedrite group through simple, cost-effective physical separation techniques—specifically magnetic and gravity separation—where the liberation size of the tetrahedrite exceeds 0.063 mm. Full article

Regulating the dissolution and interfacial behavior of minerals via external force fields is considered a promising strategy for enhancing the flotation of soluble minerals. This study explored the potential of ultrasound-assisted pulp conditioning in improving ultrafine smithsonite flotation. Specifically, we systematically evaluated the effects of ultrasonic pretreatment (UP) on the physicochemical properties of smithsonite suspensions (focusing on surface erosion behavior) and assessed subsequent flotation performance using flotation tests and modern analytical techniques. It has been found that UP can significantly modify smithsonite suspension characteristics, including particle morphology, ionic composition, electrokinetic properties, and pulp pH. Flotation results demonstrate that UP yields higher recovery compared to traditional stirring (TS) conditioning, especially at medium-to-high sodium oleate (NaOL) concentrations. Comparative analysis reveals that ultrasonic-assisted dissolution and ion-selective migration are the main factors driving improved flotation performance. Unlike TS, UP promotes greater zinc ion release, facilitates the dissolution–hydrolysis–precipitation equilibrium, generates more and finer nanoparticles in the bulk phase, and induces the deposition of hydrozincite on smithsonite surfaces. These changes increase active zinc sites for more stable NaOL adsorption, thereby enhancing the flotation of ultrafine smithsonite particles. Full article

The aim of this study is to investigate the physiological characteristics and regulatory mechanisms of porcine intramuscular fat (IMF), subcutaneous fat (take back fat (BF), for example), and visceral fat (take perienteric fat (PF), for example) to address the challenge of optimizing meat quality without excessive fat deposition. Many improved breed pigs have fast growth rates, high lean meat rates, and low subcutaneous fat deposits, but they also have low IMF content, resulting in poor meat quality. There is usually a positive correlation between intramuscular fat and subcutaneous fat deposits. This study selected eight-month-old female Tibetan pigs as experimental subjects. After slaughter, fat samples were collected. Histological differences in adipocyte morphology were observed via hematoxylin–eosin (HE) staining of tissue sections, and phenotypic characteristics of different adipose tissues were analyzed through fatty acid composition determination. Transcriptome sequencing and untargeted metabolomics were employed to perform pairwise comparisons between different fatty tissues to identify differentially expressed genes and metabolites. A siRNA interference model was constructed and combined with Oil Red O staining and lipid droplet optical density measurement to investigate the regulatory role of WNT16 in adipocyte differentiation. Comparative analysis of phenotypic and fatty acid composition differences in adipocytes from different locations revealed that IMF adipocytes have significantly smaller areas and diameters compared to other fat depots and contain higher levels of monounsaturated fatty acids. Integrated transcriptomic and metabolomic analyses identified differential expression of WNT16 and L-tyrosine, both of which are involved in the melanogenesis pathway. Functional validation showed that inhibiting WNT16 in porcine preadipocytes downregulated adipogenic regulators and reduced lipid droplet accumulation. This cross-level regulatory mechanism of “phenotype detection–multi-omics analysis–gene function research” highlighted WNT16 as a potential key regulator of site-specific fat deposition, providing new molecular targets for optimizing meat quality through nutritional regulation and genetic modification. Full article

Urban expansion in coastal areas involves infrastructure development, industrial growth, and mining activities. These coastal environments face various environmental and geological hazards that require geo-engineers to devise solutions. An integrated geophysical approach aims to address such complex challenges as sea level rise, sea water intrusion, shoreline erosion, landslides and previous anthropogenic activity in coastal settings. In this study, the proposed methodology involves the systematic application of geophysical methods (FDEM, 3D GPR, 3D ERT, seismic), starting with a broad-scale survey and then proceeding to a localized exploration, in order to identify lithostratigraphy, bedrock depth, sea water intrusion and detect anthropogenic buried features. The critical aspect is to leverage the unique strengths and limitations of each method within the coastal environment, so as to derive valuable insights for survey design (extension and orientation of measurements) and data interpretation. The coastal zone of Throrikos valley, Attica, Greece, serves as the test site of our geophysical investigation methodology. The planning of the geophysical survey included three phases: The application of frequency-domain electromagnetic (FDEM) and 3D ground penetrating radar (GPR) methods followed by a 3D electrical resistivity tomography (ERT) survey and finally, using the seismic refraction tomography (SRT) and multichannel analysis of surface waves (MASW). The FDEM method confirmed the geomorphological study findings by revealing the paleo-coastline, superficial layers of coarse material deposits and sea water preferential flow due to the presence of anthropogenic buried features. Subsequently, the 3D GPR survey was able to offer greater detail in detecting the remains of an old marble pier inland and top layer relief of coarse material deposits. The 3D ERT measurements, deployed in a U-shaped grid, successfully identified the anthropogenic feature, mapped sea water intrusion, and revealed possible impermeable formation connected to the bedrock. ERT results cannot clearly discriminate between limestone or deposits, as sea water intrusion lowers resistivity values in both formations. Finally, SRT, in combination with MASW, clearly resolves this dilemma identifying the lithostratigraphy and bedrock top relief. The findings provide critical input for engineering decisions related to foundation planning, construction feasibility, and preservation of coastal infrastructure. The methodology supports risk-informed design and sustainable development in areas with both natural and cultural heritage sensitivity. The applied approach aims to provide a complete information package to the modern engineer when faced with specific challenges in coastal settings. Full article

This paper proposes an experimental procedure based on hyperspectral imaging (HSI) combined with statistical classification for assessing archaeo-paleoanthropological stratigraphic deposits at the Gran Dolina site (TD10 unit), located in the Sierra de Atapuerca (Burgos, Spain). Representative spectral reflectance signatures were determined and analyzed using HSI measurements and statistical classification methods in natural light conditions across various capture distances. This study aims to characterize and quantify cave sediments by defining spectral models for feature classification and spectral similarity analysis, evaluating the strengths and limitations of spectral captures at this specific site. HSI technology enhances the analysis and identification of materials at an internationally recognized reference site for human evolution studies. Hyperspectral imaging assessment of archaeo-paleoanthropological stratigraphic deposits emerges as an innovative digital tool, revolutionizing the sustainable management of cultural heritage and environmental sciences by enabling advanced material identification and stratigraphic analysis. Full article

Solar-driven interfacial evaporation desalination is regarded as a promising solution to address freshwater scarcity. However, salt deposition remains a significant challenge. While structural designs such as designated deposition sites can control crystallization, the mechanisms of salt precipitation at specific locations are still unclear. In the present work, we designed a three-dimensional conical evaporator using low-cost cellulose paper for efficient solar-driven desalination. This innovative evaporator design achieves controlled salt crystallization by meticulously balancing the rates of salt diffusion and accumulation, thereby directing salt precipitation to a predetermined location approximately 1.4 cm above the conical base. This phenomenon arises from temperature variations across the evaporator’s three-dimensional surface, which induce differences in water surface tension and create favorable sites for salt precipitation. Such a salt management strategy allows for continuous operation for up to 8 h in high-salinity conditions (24.5 wt.%) without compromising performance. Under one sun irradiation, the evaporator demonstrates exceptional performance, with an evaporation rate of 2.54 kg·m⁻²·h⁻¹ and an impressive energy conversion efficiency of 93.7%. This approach provides valuable insights into the salt precipitation mechanism, contributing to the future design of three-dimensional evaporators and innovative salt collection strategies. Full article