Search Results (358)

On the North China Plain, the winter wheat season is poorly synchronized with precipitation, making the traditional winter wheat–summer maize system heavily dependent on supplemental irrigation and associated carbon inputs. Based on a split-plot field experiment in Shenzhou, Hebei, from October 2022 to October 2025, this study evaluated the trade-off between annual system yield and area-scaled carbon emission among six cropping systems under conventional irrigation (CK) and rainfed management (R). The winter wheat–summer maize system (WM) maintained the highest grain-oriented annual system yield (22.91 t ha⁻¹ yr⁻¹ under CK), but it also showed the highest area-scaled carbon emission (11.97 t CO₂-eq ha⁻¹ yr⁻¹). The winter wheat–summer maize–spring maize system (WMM) reduced area-scaled carbon cost relative to WM (8.97 vs. 11.97 t CO₂-eq ha⁻¹ yr⁻¹ under CK), whereas its product-scaled carbon footprint remained comparable to or slightly higher than that of WM. Under a unified dry-matter basis, the double silage-maize system (FM) showed the lowest dry-matter-scaled carbon footprint (CF_DM; 193.85 and 175.71 kg CO₂-eq t DM⁻¹ under CK and R, respectively). Soil respiration in 2025 varied mainly with observation date and cropping-system configuration, and soil organic carbon (SOC) stock at the 2025 harvest differed among cropping systems, water-management regimes, and soil depths. Overall, WM remained the highest-yielding option under a grain-supply objective, whereas FM, the ryegrass–early-summer maize system (RM), and the forage winter wheat–early-summer maize system (FWM) were relatively more suitable under multifunctional biomass-supply and low-carbon-transition objectives. Full article

Silage is a fundamental component of cattle feed, and its microbiological quality is critical for animal health and human safety. Improper ensiling conditions, such as oxygen exposure or inadequate acidification, can promote the growth of pathogens like Listeria monocytogenes, Clostridium botulinum, and Bacillus cereus. This study aimed to evaluate the microbial status of maize silages and identify pre-ensiling factors influencing its hygienic safety. Over a four-year period, 406 silage samples were collected from cattle farms across Poland. The research evaluated general hygiene indicators and screened for specific pathogens using standard culture methods, polymerase chain reaction toxotyping, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The impact of agricultural practices, including soil quality, organic fertilization, and microbial inoculation, was also analyzed. The analysis revealed that 32.1% of silages fell outside the reference pH range, indicating potential aerobic instability. While Salmonella and Campylobacter were not detected, Clostridium spp. were highly prevalent (81.0%), and C. perfringens was confirmed in 24.9% of samples. Listeria species occurred in 2.9% of silages, with L. innocua being the most frequent isolate. Statistical analysis showed that organic fertilization was significantly linked to specific C. perfringens toxotypes, though it did not increase the overall microbial burden. Conversely, microbial inoculation generally reduced the counts of several undesirable bacteria, although these differences were not statistically significant across all parameters. High pH values and significant contamination with Clostridium, B. cereus, and fungi remain critical challenges for silage safety. The results underscore the necessity for improved agricultural practices—specifically the minimization of soil and manure contamination during harvest—and the broader adoption of microbial inoculation to ensure the microbiological stability of fermented forage. Full article

While silage maize (Zea mays L.) remains the dominant biogas feedstock crop in Germany, concerns about landscape homogenization and ecological risks have stimulated the search for more diverse energy crops. This study evaluated twelve amaranth genotypes (GT01–12; Amaranthus spp.) in southwest Germany using field experiments combined with biomass composition analysis and laboratory batch biogas assays. In contrast to earlier studies focusing primarily on the cultivar ‘Baernkraft’ (GT04), a broader set of genetic material was examined. Significant differences among GTs were observed for plant density, dry matter yield (DMY), dry matter content (DMC), and biomass composition. The most productive genotypes (GT09 and GT11) exceeded 10 Mg ha⁻¹ DMY, clearly outperforming Baernkraft. However, even these GTs did not reach the ≈28% DMC threshold considered necessary for reliable ensiling. Lignin concentrations ranged from 4.7% to 7.2% of dry matter. Methane concentrations remained relatively stable (54–55%), resulting in an average methane yield of 1788 ± 441 m³ CH₄ ha⁻¹ (maximum: 2677.8 m³ CH₄ ha⁻¹) across all genotypes and harvest dates. These findings indicate that amaranth may contribute to diversification of biogas cropping systems, although its agronomic and substrate-related performance remains inferior to that of maize under the conditions studied. Full article

The field experiment was conducted in 2016–2018 at the Department of Agronomy of the Poznań University of Life Sciences on the fields of the Research and Education Centre in Gorzyń, Złotniki branch. It was a single-factor experiment with six sowing dates of an ultra-early maize variety: A1—12 April, A2—26 April, A3—10 May, A4—24 May, A5—7 June, and A6—21 June. Seeds of the maize variety ‘Pyroxenia’ were used in the experiment. This variety is characterized by extremely early maturity (FAO 130), rapid initial development and elongation growth. Delaying the maize sowing date from A1 to A2 resulted in a 16.5% reduction in starch content in the silage dry matter, and a 14.6% increase in the ADF (Acid Detergent Fiber) fiber fraction. The difference in milk production per hectare between maize sown on date A1 and date A6 was 14,189.51 kg/ha, representing 97.1%. Delaying the maize sowing date led to an increase in the abundance of Clostridium spp. in silages, which are responsible for increased losses of dry matter, including starch. No butyric acid was detected in the silages as a final product of butyric fermentation. The low abundance of bacteria from the family Enterobacteriaceae in the silages indicated that they were well prepared. Silages prepared from maize sown at later dates were characterized by a higher abundance of undesirable mold fungi, which are responsible for dry matter losses, including starch. The coefficient of determination showed that 38.54% of the variation in silage starch content was explained by variation in mold abundance in the silage. According to the Flieg–Zimmer scale, all silages received a very good rating, regardless of maize sowing date. Full article

Maize biomass production and quality are influenced by numerous factors, including fertilization, soil characteristics, and climatic conditions. The aim of our study was to evaluate how different fertilization treatments ((1) Control, (2) farmyard manure (FYM), (3) FYM with added mineral nitrogen (FYM + N), and (4) FYM with added NPK mineral fertilizers (FYM + NPK)) affect the biomass yield and quality parameters (crude protein (CP), fiber content (FC), neutral detergent fiber (NDF), starch content (STR), organic matter digestibility (OMD), and neutral detergent fiber digestibility (DNDF)) of silage maize under various soil and climatic conditions in the Czech Republic (Caslav—degraded Chernozem, Ivanovice na Hané–Chernozem, Lukavec–Cambisol). The experiment was conducted from 2020 to 2023. Additionally, the study analyzed the effects of fertilization on soil chemical properties (pH, P, K, Ca, Mg, C, N). The highest average biomass yields were recorded in Ivanovice (23.8 t ha⁻¹, A), followed by Lukavec (19.7 t ha⁻¹, B) and Caslav (18.1 t ha⁻¹, B). Comparing fertilizer treatments, no significant differences were observed among FYM, FYM + N, and FYM + NPK; however, all three treatments significantly outperformed the Control at all sites. Conversely, fertilization did not affect the quality parameters. For silage maize, FYM represents the optimal fertilization strategy, providing yields and quality comparable to the combined application of mineral N, P, and K, which are more costly (in terms of purchase and application) and, under certain conditions, may negatively impact the environment. Nevertheless, the application of mineral fertilizers increased soil nutrient content, thereby improving conditions for subsequent crops. Full article

Aims: This study aimed to evaluate how the combined application of slow-release nitrogen fertilizer and urea influences nitrogen losses via runoff and the yield performance of silage maize in red soil sloping cropland, in order to support region-specific strategies for soil and water conservation and efficient nitrogen management in maize-based systems. Methods: A one-year field runoff plot experiment was conducted under natural rainfall conditions. Three fertilization treatments with equal nitrogen input were established: conventional urea application (CK), 25% controlled-release nitrogen fertilizer combined with 75% urea (P1), and 50% controlled-release nitrogen fertilizer combined with 50% urea (P2). Results: (1) Co-application of slow-release fertilizer and urea significantly improved soil and water conservation compared to CK; total runoff was reduced by 9.17% and 8.29% under P1 and P2, respectively; and soil loss was reduced by 16.45% and 12.25%. (2) The co-application of slow-release fertilizer effectively reduced nitrogen losses from sloping farmland. Compared to CK, both P1 and P2 reduced losses of total nitrogen (TN), nitrate nitrogen (NO₃⁻-N), and ammonium nitrogen (NH₄⁺-N) during the maize growth period, with the reductions under P2 being most pronounced. (3) The co-application of slow-release fertilizer and urea enhanced maize nitrogen uptake and increased maize yield. Conclusions: Based on the results of this single-year field experiment, the combined application of controlled-release nitrogen fertilizer and urea showed potential advantages in reducing runoff-related nitrogen losses while improving crop productivity in red-soil sloping cropland. Among the treatments, P2 (50% controlled-release nitrogen substitution) exhibited relatively better overall performance. However, the broader applicability of these findings requires further verification through multi-year field experiments under different climatic conditions. Full article

The study evaluated the effects of replacing soybean meal (SBM) with marula oilcake (MOC) at equal inclusion (10% fresh weight) levels in whole-crop maize silage treated with or without lactic acid bacteria inoculants on fermentation characteristics, nutritive value, aerobic stability, and in vitro nutrient degradability. Maize was ensiled with SBM or MOC in a non-iso-nitrogenous 2 × 3 factorial design and either inoculated or uninoculated with Lalsil Fresh or Sil-All 4×4 for 90 days. Protein sources differed significantly (p < 0.05). The MOC showed high DM, EE, GE, and ADL, whereas SBM had high CP, ash, and IVOMD. Fibre fractions (aNDF and ADF) were similar (p > 0.05). The SBM control showed significantly high (p < 0.05) LA, NH₃-N, CP, IVOMD, propionic acid, and early gas production, indicating efficient fermentation. The SBM + Lalsil maintained low pH, and early OM, CP, and GE degradability. The SBM + Sil-All achieved the highest (p < 0.05) OM, NDF, and ADF degradability and acetic acid production than other treatments. The MOC control showed low (p < 0.05) pH, high fibre and GE, reduced butyric acid, and low 48 h gas production, indicating slower fermentation but improved stability. The MOC + Lalsil had high (p < 0.05) DM, low CO₂ and yeasts and moulds, and the highest (p < 0.05) CP degradability, propionic acid, and peak gas production at 12 h. The MOC + Sil-All showed high (p < 0.05) GE and WSC with peak GE degradation at 12 h, but low NDF degradability and reduced gas production. Overall, SBM improved degradability and fermentation efficiency, particularly with Sil-All, whereas MOC enhanced energy density and aerobic stability, with Lalsil optimising protein utilisation. Matching inoculant type to protein source is essential to optimise silage quality and rumen fermentation. Further research should assess different inoculant inclusion rates and include a maize-only control, and evaluate protein source inclusion under iso-nitrogenous conditions to allow more accurate comparisons. Full article

The quality of beef fat depends on both intramuscular fat (IMF) content and fatty acid (FA) composition, which can be modulated by finishing diets. This study evaluated the effects of silage type and feeding intensity on IMF deposition, FA profile, desaturase indices, and lipid quality indices in finishing Holstein–Friesian bulls. Thirty-two bulls were assigned to a 2 × 2 factorial design (n = 8/group) and fed total mixed rations for 120 days based on grass silage or maize silage, under intensive (≈50:50 forage:concentrate, DM basis) or semi-intensive feeding (≈70:30). FA composition of longissimus lumborum lipids was determined by GC-FID, and lipid quality indices were calculated, including the atherogenic index (AI), thrombogenic index (TI), and the hypocholesterolemic/hypercholesterolemic ratio (h/H). Feeding intensity increased IMF content (p = 0.001) and the absolute amounts of major FA classes (g/100 g meat). Silage type primarily affected FA composition by increasing n-3 PUFA and lowering the n-6/n-3 ratio in grass silage diets (p = 0.042). Several FAs showed silage type × feeding intensity interactions (p < 0.05), indicating that the response to dietary energy supply depended on the forage base. Overall, feeding intensity mainly regulated lipid deposition, whereas silage type modulated the nutritional profile of intramuscular fat. Full article

Nutrient surpluses in regions with intensive livestock farming challenge sustainable crop production and have driven interest in alternative fertilization strategies and microbial biostimulants. Although microbial inoculation (MO) has been extensively studied in plant production, its agronomic relevance under field conditions remains controversial due to inconsistent outcomes. To address these inconsistencies, we conducted three-year field trials on two well-fertilized sandy sites in northern Germany. A microbial consortium consisting of Rhizoglomus irregulare, Funneliformis mosseae, Funneliformis caledonium, and Bacillus atrophaeus Abi05 was applied to silage maize (cultivar Amaroc S230) under contrasting fertilization regimes. In two of three years, microbial inoculation increased dry mass yield in the absence of starter fertilization, whereas both a high nutrient input variant (100 kg ha⁻¹ diammonium phosphate, DAP) and a lower nutrient input organo-mineral microgranular fertilizer (25 kg ha⁻¹) suppressed inoculant effects. Notably, yields from plots amended solely with the microbial inoculant reached at least the same level as those obtained with starter fertilization. In the third year, under drought conditions, defined as soil water contents below 10% in the 0–30 cm depth, no positive yield responses to microbial inoculation were observed. Quantitative PCR-based analyses of pre-sowing soils revealed that the abundances of Firmicutes, β-Proteobacteria, and total fungi were associated with yield responses, with Firmicutes and β-Proteobacteria showing negative and fungi showing positive correlations; together, these microbial predictors explained 38% of the variance in inoculant-induced yield response. Our findings demonstrate that soil microbiome characteristics can predict inoculant performance and that microbial inoculation is most effective without starter fertilization and under adequate soil moisture. Full article

This study aims to investigate the effects of three lactic acid bacteria (LAB) strains, Lactiplantibacillus plantarum, Lactiplantibacillus pentosus and Limosilactobacillus fermentum, isolated from traditional pickles in Guizhou, on the fermentation process and microbial community dynamics of ensiled whole-plant maize, soybean, and their mixtures. The results revealed that compared to the CK group, the lactic acid levels of Lactiplantibacillus plantarum and Lactiplantibacillus pentosus were significantly increased in the treatment groups (p < 0.05), resulting in a faster pH reduction, along with decreases in ammonia nitrogen (AN) and butyric acid (BA) content. In contrast, the Limosilactobacillus fermentum treatment (p < 0.05) promoted acetic acid (AA) production and inhibited the growth of harmful microbiota in soybean silage. Notably, inoculation with all LAB strains enhanced the aerobic stability of maize silage by promoting the proliferation of Lactiplantibacillus during the later stages of fermentation, thereby sustaining a low pH and mitigating the depletion of water-soluble carbohydrates (WSC). Furthermore, all treatments accelerated silage fermentation by enhancing the LAB population and competing with yeast and Escherichia coli for available nutrients in mixed silage. These findings indicate that three LAB strains, when used as microbial additives, demonstrated potential to improve silage quality in the Karst region. Full article

Maize silage can play a key role in policies aimed at stabilising local energy systems, as it constitutes a critical renewable feedstock for European biogas plants. By providing a dense and predictable source of chemical energy, it supports balance and reliability in the agricultural energy sector. To convert this potential into stable energy production, operators require kinetic models that translate routine silage quality indicators into concrete guidance for digester operation and control. Therefore, the aim of this article was to evaluate the batch kinetics of anaerobic digestion (AD) of maize silage and to select an adequate model for describing biochemical methane potential (BMP) profiles and associated energy recovery in the context of start-up, organic loading rate (OLR), hydraulic retention time (HRT) and feedstock preparation. Ten batches of silage (A–J) were examined, covering a realistic range of pH, electrical conductivity (EC), dry and volatile solids, ash, protein–fat–fibre fractions, fibre composition (NDF, ADF and ADL), derived fractions (hemicellulose, cellulose, and residual organic matter (OM)), C/N ratio and macro-/micronutrient profiles, including trace elements relevant to methanogenesis (Ni, Co, Mo, and Se). BMP tests were carried out in batch mode, and the resulting curves were fitted using the modified Gompertz and a first-order kinetic model. Methane yields of approx. 100–120 m³ CH₄/Mg fresh matter (FM) and 336–402 m³ CH₄/Mg volatile solids (VS), with CH₄ contents of 52–57% v/v, were typical for energy-grade maize silage. Kinetic and energetic behaviours were governed mainly by residual OM and hemicellulose (shortening the lag phase and increasing the maximum methane production rate), the ADL/cellulose ratio (controlling the slower hydrolytic tail), EC and Na/Cl/S (extending the lag phase), and C/N together with Ni/Co/Mo/Se (stabilising methanogenesis). The modified Gompertz model reproduced BMP curves with a pronounced lag phase and asymmetry more accurately (lower error and better information criterion values), and its parameters directly support start-up design, OLR ramp-up and energetic performance optimisation in bioenergy reactors. The novelty of this work lies in combining batch BMP tests, comparative kinetic modelling and detailed silage characterisation to establish quantitative links between kinetic parameters and routine maize silage quality indicators that are directly relevant for biogas plant operation and renewable energy production. Full article

A field experiment was conducted in 2019–2020 and 2020–2021 at Rogoza, Fala, and Brežice in Slovenia to examine the biological viability of a mixed intercropping system and the effect of winter catch crops (WCCs) on maize growth parameters. The experiment included Italian ryegrass (IR) in pure stands, fertilized with nitrogen (N) in spring (70 kg N ha⁻¹), mixtures of crimson clover and red clover 50:50 (C), and intercropping between IR and C (IR+C). Neither mixture was fertilized with N in spring. We evaluated different competition indices and biological efficiency. Relative crowding coefficient (RCC) and actual yield loss (AYL) exceeded 1, indicating a benefit of IR+C intercropping. The IR in intercropping was more aggressive, as indicated by positive aggressivity (A) and a competitive ratio (CR) > 1, and it dominated over C in IR+C (that had negative A values and CR < 1). The competitive balance index (Cb) differed from zero, the relative yield total (RYT) was 2.24, the land equivalent coefficient (LEC) exceeded 0.25, the area–time equivalent ratio (ATER) exceeded 1, and land use efficiency (LUE) exceeded 100%. IR+C exhibited the highest total aboveground dry matter yield of maize (29.22 t ha⁻¹), the highest nitrogen content in dry matter grain yield of maize (206.35 kg ha⁻¹), the highest nitrogen and potassium content in maize stover (105.7 and 105.7 kg ha⁻¹, respectively), and the highest nitrogen and potassium content in the total aboveground dry matter of maize (312 and 267.3 kg ha⁻¹, respectively). The C/N ratio in dry matter yield of IR was 45.35, and in IR+C it was 33.43, which means that the mixture had a positive effect on nutrient release in maize. The ryegrass–clover mixture, according to the calculated biological indices, had advantages over pure stands and had a positive effect on maize yield. Full article

To reconcile the intensifying trade-off between chronic water scarcity and escalating forage demand in the Yellow River Basin, this study optimized integrated irrigation and fertilization regimes for silage maize. Leveraging the AquaCrop model, validated by 2023–2024 field experiments and a 35-year (1990–2024) meteorological dataset, we systematically quantified the impacts of multi-factorial water–fertilizer–heat stress under drip irrigation with mulch (DIM) and shallow-buried drip irrigation (SBDI). Model performance was robust, yielding high simulation accuracy for soil moisture (RMSE < 3.3%), canopy cover (RMSE < 3.95%), and aboveground biomass (RMSE < 4.5 t·ha⁻¹), with EF > 0.7 and R² ≥ 0.85. Results revealed distinct stress dynamics across hydrological scenarios: mild temperature stress predominated in wet years, whereas severe water and fertilizer stresses emerged as the primary constraints during dry years. To mitigate these stresses, a medium fertilizer rate (555 kg·ha⁻¹) was identified as the stable optimum, while dynamic irrigation requirements were determined as 90, 135, and 180 mm for wet, normal, and dry years, respectively. Comparative evaluation indicated that DIM achieved maximum productivity in wet years (aboveground biomass yield 70.4 t·ha⁻¹), whereas SBDI exhibited superior “stable yield–water saving” performance in normal and dry years. The established “hydrological year–irrigation method–threshold” framework provides a robust decision-making tool for precision management, offering critical scientific support for the sustainable, high-quality development of livestock farming in arid regions. Full article

Maize is a water-intensive crop widely cultivated in temperate regions, where irrigation practices strongly influence its environmental performance. This study applies Life Cycle Assessment (LCA) to compare the environmental impacts of surface and drip irrigation for maize green silage production in the Po Valley (Italy), following ISO 14040/44 standards and adopting a cradle-to-farm-gate perspective. Results show that, compared to drip irrigation, surface irrigation leads to lower impacts in 14 out of 15 categories, with reductions ranging from −0.2% (marine eutrophication) to −61% (human toxicity, non-cancer), particularly for human toxicity and resource use due to lower diesel and infrastructure requirements. Conversely, drip irrigation achieves a 58% reduction in water use thanks to its higher irrigation efficiency. The single-score assessment highlights water use as the key differentiating factor, positioning drip irrigation as preferable under scenarios of water scarcity. Contribution and sensitivity analyses confirm that nitrogen fertiliser use and mechanisation are major hotspots, while yield variation (±30%) significantly affects the magnitude of results. These findings emphasise a clear trade-off: surface irrigation shows a lower environmental burden across most impact categories, whereas drip irrigation strongly reduces water scarcity impacts and provides robust, site-specific evidence to guide sustainable irrigation strategies in intensive maize systems. Full article

Organic production systems impose strong environmental constraints on silage maize, yet the relative contributions of genotype, environment and their interaction (G × E) to key performance traits remain insufficiently resolved. This study evaluated six maize cultivars across 11 organically managed environments (location × year combinations) in Poland, assessing weed infestation, plant height, fresh matter yield, dry matter content and dry matter yield. Genotype × environment interaction was explicitly analyzed using AMMI-based models, and cultivar adaptability and stability were evaluated using complementary indices. Environmental effects consistently dominated all traits, explaining 78–91% of total variation, while G × E interactions, though smaller, were significant and altered cultivar rankings. Weed infestation ranged widely across environments, from below 10% to over 90%, and was almost entirely environment-driven. Yield-related traits followed a strong precipitation gradient, with Pawłowice and Śrem showing the highest biomass potential. SM Perseus produced the greatest dry matter yields (13.53 t·ha⁻¹), whereas SM Mieszko combined high dry matter content (37.73%) with outstanding stability. Mega-environment analysis identified distinct adaptive niches, confirming that no genotype performed consistently best across all conditions. These findings close a key knowledge gap regarding cultivar performance under organic management and demonstrate the necessity of multi-environment evaluation that integrates performance, stability and adaptability analyses to support site-specific cultivar recommendations that enhance biomass productivity and silage quality in ecologically managed maize systems. Full article