Search Results (85)

Conventional water treatment relies on sequential flocculation and disinfection, which inflates infrastructure costs and heightens the risk of disinfection byproduct formation. Here, we report a magnetic lignin-based flocculant (LS-DMC-AM@Fe₃O₄) that integrates flocculation, sterilization, and rapid magnetic separation within a single material. The composite was synthesized by thermally initiated graft copolymerization of methacryloyloxyethyl trimethylammonium chloride (DMC) and acrylamide (AM) onto sodium lignosulfonate (LS), followed by incorporation of Fe₃O₄ nanoparticles (NPs) at 15 wt% loading; the product exhibited a saturation magnetization of 12.8 emu g⁻¹. LS-DMC-AM@Fe₃O₄ achieved 98.2% kaolin turbidity removal at 1 mg L⁻¹ and 98.6% E. coli removal at 8 mg L⁻¹, and displayed a markedly broader effective dosage window than its non-magnetic analog. We attribute this broadened window to Fe₃O₄-enhanced membrane disruption, which liberates anionic intracellular contents that buffer excess cationic charge and thereby suppress restabilization. The bactericidal efficiency reached 90% at 18 mg L⁻¹, 1.6-fold higher than LS-DMC-AM, governed by a synergistic dual mechanism: quaternary ammonium contact-killing coupled with Fe₃O₄ NP-induced intracellular reactive oxygen species (ROS) accumulation. Under an external magnetic field, flocs underwent rapid phase separation and displayed enhanced shear-regrowth capacity (E. coli floc recovery factor: 53% vs. 26%); Fe₃O₄ NPs were recovered at >95% efficiency over two cycles. Despite higher unit production costs, LS-DMC-AM@Fe₃O₄ delivers competitive per-unit-volume treatment economics through its ultralow effective dosage and magnetic seed recyclability. These results establish a viable strategy for engineering multifunctional, recyclable flocculants from industrial lignin waste. Full article

The seedling production stage of lettuce (Lactuca sativa L.) is crucial for crop success, as it determines the initial quality of the plants. The use of seeds with rapid and uniform germination is essential to ensure proper seedling establishment. Among sustainable alternatives for water management, irrigation with ozonated water stands out due to its disinfectant potential and its ability to stimulate plant physiology. This study evaluated the effects of irrigation with ozonated water on the production and development of lettuce seedlings. The experiment was conducted in a completely randomized design (CRD) arranged in a 2 × 2 factorial scheme, with four replications. Two lettuce cultivars were tested: one with smooth leaves and another with crisp leaves. The variables analyzed included germination parameters (final percentage, germination index and mean germination rate, uniformity, and time to reach 10, 50, and 90% germination), as well as initial growth parameters (total height, shoot and root height, and dry matter content). Analyses were performed on 20 seedlings per cultivar. Irrigation with ozonated water promoted significant growth (p < 0.05) of the shoot and root growth, with increases of 16.90 and 4.99% for the smooth-leaf cultivar, and 24.27 and 9.26% for the crisp-leaf cultivar, compared to the control. Ozone application did not alter the microbiological, physical, or chemical parameters of the water. These growth-promoting effects are likely associated with increased oxygenation of the root zone, enhanced oxidation of organic matter in the substrate, and improved nutrient availability promoted by ozone-derived radicals, which may also optimise root respiration and reduce pathogenic pressure. The applied concentration of 5 mg L⁻¹ O₃ over a 25-day seedling production cycle proved effective and did not cause phytotoxic effects. Irrigation with ozonated water is an efficient and environmentally safe alternative for producing vigorous lettuce seedlings. Full article

As agriculture moves towards green transformation and low-carbon production, the high energy consumption, environmental burden, and residue risks associated with conventional chemical fertilisers, pesticides, and disinfectants have become increasingly prominent. Non-thermal plasma (NTP) can generate reactive oxygen and nitrogen species (RONS) under near-ambient temperature and pressure conditions, while offering low chemical residue, high reactivity, and modular equipment design. It has therefore attracted growing attention in agricultural engineering and green agricultural input preparation. This review focuses primarily on studies published within the past five years, together with the selected foundational literature retrieved from Web of Science, Scopus, PubMed, MDPI, and ScienceDirect. It systematically examines the fundamental mechanisms, application modes, and representative agricultural scenarios of NTP, with particular emphasis on agricultural nitrogen fixation and fertilisation, seed treatment and seedling raising, crop growth regulation and protection, soil improvement and remediation, and postharvest preservation and safety treatment of agricultural products. Key technological advances are then summarised, including optimisation of discharge systems and reactor configurations, plasma–catalysis synergy, preparation of plasma-activated water (PAW) and plasma-activated mist (PAM), and the development and integration of specialised agricultural equipment. In addition, the current state-of-the-art (SOA) of artificial intelligence (AI) applications in plasma-process modelling, process-parameter optimisation, agricultural performance evaluation, and intelligent control is discussed. Existing evidence indicates that NTP is particularly relevant to controlled-environment agriculture, including greenhouse cultivation, hydroponics, and aeroponics, where discharge processes, water or nutrient solutions, and crop root-zone management can be coupled for in situ nitrogen supply, activated-medium preparation, and crop protection. However, reported effects remain strongly dependent on discharge type, energy input, reactive-species composition, treatment dose, crop species, cultivation system, and application route. Therefore, NTP-based agricultural technologies should be evaluated using consistent indicators, including energy consumption, product selectivity, reactive-species stability, treatment throughput, crop response, ecological safety, and system-level integration with AI and IoT. Future research should prioritise high-efficiency reactors, standardised evaluation frameworks, cross-scale mechanistic understanding, reliable datasets, and closed-loop intelligent control, thereby supporting the transition from laboratory studies to reproducible and application-oriented agricultural systems. Full article

Biocoagulants derived from Moringa oleifera Lam seeds are a sustainable alternative for water clarification, but require preparation adapted to the treatment of the dark waters of the Amazon River. This study compared the effectiveness of three coagulant preparation methods from moringa seeds: powder (B1), saline solution (B2), and aqueous solution (B3) in the clarification of samples collected on the shore of Macapá (Amapá—AP), Brazil. The tests were performed using a jar test (fast mixing of 100 rpm for 3 min and slow mixing of 20 rpm for 15 min), with dosages of 20 to 200 mg·L⁻¹, and sedimentation times between 10 and 60 min. The optimized conditions were: 80 mg·L⁻¹/20 min (B1), 40 mg·L⁻¹/30 min (B2), and 40 mg·L⁻¹/40 min (B3). The maximum removals achieved by clarification were as follows: apparent color (92.6%), turbidity (79.4%), chloride (70.9%), ammonia (81.2%), aluminum (99.1%), copper (85.4%), iron (85.8%), and manganese (100.0%). The saline solution was the most efficient. Filtration brought additional improvements to the treated water. It was found that the moringa coagulant was effective in removing contaminants from the waters of the Amazon River, standing out as a green, sustainable, and low-cost technology. However, disinfection would be necessary to improve its microbiological quality. Full article

Lilium, a traditional plant with dual medicinal and ornamental values, is restricted in its industrial development by the low natural propagation rate of bulbs and dependence on imported high-quality germplasm. To address this bottleneck, this study used new lines of LA hybrid lilies and Asiatic hybrid lilies (three cultivars) as experimental materials to establish an efficient and stable tissue culture and rapid propagation system. Key procedures including disinfection of different explants (bulb scales and capsules), adventitious bud induction and proliferation, rooting culture, as well as acclimatization and transplantation were systematically evaluated. The results showed that bulb scales were superior to capsule seeds as explants in tissue culture (contamination rate 9.44%, regeneration rate 11.92%). After disinfection with 75% ethanol combined with 10% sodium hypochlorite, the contamination rate could be controlled at 14.29–21.43%, and the regeneration rate reached 100%. Supplementation with 50 g·L⁻¹ sucrose + 1 mg·L⁻¹ 6-BA + 0.1 mg·L⁻¹ NAA (Treatment TA1), 50 g·L⁻¹ sucrose + 2 mg·L⁻¹ 6-BA + 0.5 mg·L⁻¹ NAA (Treatment TA4) in MS medium, combined with dark culture, could effectively promote adventitious bud induction, proliferation and bulblet enlargement. For the rooting stage, the optimal media were 1/2 MS + 0.5 g·L⁻¹ activated charcoal + 2 mg·L⁻¹ 6-BA + 0.5 mg·L⁻¹ NAA (Treatment TB4) or MS + 0.3 g·L⁻¹ activated charcoal + 2 mg·L⁻¹ 6-BA + 0.5 mg·L⁻¹ NAA (Treatment TB1), and the highest rooting rate of ‘Pink Renault’ reached 100%. When plantlets from all three cultivars were combined and acclimatized and transplanted into sterilized peat soil, the overall survival rate was 89.33%. The TOPSIS method was also adopted for comprehensive evaluation to screen out the optimal culture conditions for different varieties. Based on phenotypic observation and physiological index data, ‘Pink Renault’ showed great potential as an excellent propagation germplasm. The integrated and optimized technical system provides a feasible solution for large-scale and industrialized seedling production of medicinal and ornamental lilies, and is of great practical significance for the efficient utilization of germplasm resources and sustainable development of the lily industry. Full article

Modern agriculture faces significant challenges, such as population growth, the reduction in productive agricultural land, and, most importantly, climate change. To address these issues, non-thermal plasma treatment of seeds and plants has emerged as a promising alternative to conventional chemical-based methods. This advanced technology, a powerful chemical reactor in the gas phase, has various applications, from stimulating seed germination and plant growth to controlling pathogens. The effects of non-thermal plasma on seeds include morphological and chemical changes in the seed coat, increased permeability and water uptake, and the activation of some internal biochemical mechanisms. Studies have demonstrated improvements in germination, plant development, and the activation of internal biochemical mechanisms with the intensified production of secondary metabolites. Non-thermal plasma also contributes to reducing the microbial load, providing an effective and environmentally friendly method of disinfection. This review synthesises the current knowledge on non-thermal plasma sources used in plasma agricultural applications for seed treatments, emphasising that in some cases the exposure of seeds to such discharge stimulates germination and also promotes early seedling growth. In addition, it highlights reported biochemical and nutraceutical improvements, including changes in antioxidant capacity, phenolic content and other bioactive compounds which add considerable value to the resulting plants. Finally, the decontamination potential is discussed, along with results discussing the potential of NTP to decontaminate seeds, associated with an extension to the shelf-life of products and identifying key challenges and research gaps for implementing this technology in agricultural practices. The integration of this technology into modern agriculture, including vertical farms and hydroponic systems, opens up the prospect for more sustainable and productive agriculture. However, scaling up the process and optimising processing parameters remain important challenges that require further attention, research and technological development. Full article

Soil-borne diseases have become increasingly serious due to continuous planting. Soil fumigation may be inadequate because of the persistence of soil-borne pathogens on ginger seed rhizome. A combined strategy of soil fumigation and seed rhizome disinfection would be necessary to achieve synergistic control. In this study, the approach of soil fumigation with chloropicrin (CP) coupled with seed rhizome disinfection (Copper, Cu) was first adopted to evaluate the synergistic effects on soil physicochemical properties, enzyme activities and microbial communities, and therefore reveal mechanisms for soil microecological health and crop yield promotion. The results showed the comprehensive strategy could reduce NO₃⁻-N content, and the activities of soil enzymes, while increased NH₄⁺-N content, EX-Cu, and OXI-Cu content, which were positively correlated with ginger yield but negatively correlated with soil-borne pathogens and plant mortality. On the other hand, there was a reduction in bacterial diversity and richness, which was positively correlated with the abundance of soil-borne pathogens. Moreover, some beneficial soil microorganisms’ relative abundance (such as Firmicutes, Actinobacteria, Bacillus, and Sphingomonas.) was increased. The strategy decreased the abundance of Fusarium spp. and Phytophthora spp. by 49.41–90.07% and 43.34–89.21%, respectively. Compared with other treatments, the combination decreased the ginger mortality by 5.70–57.02% and increased the growth of ginger plants and yield by 3.58–139.96%, and 13.11–399.74%, respectively. This study highlights a prospect to promote ginger growth and yield by blocking the transmission of primary infection pathogens in ginger cultivation and improving soil ecological environment. Full article

Metal and metal oxide nanoparticles (NPs) synthesized through biologically mediated reduction of metal ions using biomolecules derived from microorganisms, algae, or plants are attracting growing attention in plant biotechnology due to their multifunctional properties and environmental advantages compared with conventional physicochemical synthesis. This review provides a comprehensive analysis of biological approaches for NP production using bacteria, fungi, algae, cyanobacteria, whole plants, and in vitro plant cell cultures. The main biosynthetic mechanisms, types of reducing and capping metabolites, metal specificity, and typical NP characteristics are described for each system, with emphasis on their relative productivity, scalability, reproducibility, and biosafety. Special consideration is given to plant cell and tissue cultures as highly promising platforms that combine the metabolite diversity of whole plants with precise control over growth conditions and NP parameters. Recent advances highlight the significance of bioengineering of reductive capacity as a novel strategy to enhance the efficiency and controllability of NP biosynthesis. Since NP formation is driven by key biomolecules, targeted modification of biosynthetic pathways through metabolic and genetic engineering can substantially increase NP yield and allow fine-tuning of their structural and functional properties. The applications of biogenic NPs in plant biotechnology are systematically evaluated, including their use as environmentally safe disinfectants for explants and seed sterilization, modulators of callus induction and morphogenesis, and abiotic elicitors that enhance the accumulation of economically valuable secondary metabolites. Remaining challenges, such as variability in NP characteristics, limited scalability, and insufficient data on phytotoxicity and environmental safety, are discussed to outline future research priorities. The synthesis–function relationships highlighted here provide a foundation for developing sustainable NP-based technologies in modern agriculture. Full article

This study examined the effects of dielectric barrier discharge (DBD) plasma and copper oxide nanoparticles (CuO NPs) on rice seed quality, seedling growth, and fungal inhibition. Sanpatong 1 rice seeds were treated with DBD plasma at three exposure durations (0.4, 0.6, and 0.8 s/cm) and coated with CuO NP solutions at five concentrations (0, 0.02, 0.04, 0.06, and 0.08 M). The experiment followed a split-split-plot design within a randomized complete block design (RCBD), with storage time (0, 2, 4, and 6 months) as the main plot factor. Plasma etching improved seed surface wettability, while CuO NPs increased copper uptake and promoted growth at 0.04–0.06 M but caused toxicity at 0.08 M. Combined treatments suppressed Rhizopus sp. and Rhizoctonia solani, though Aspergillus spp. were less affected. Seed quality declined after six months of storage, likely due to oxidative stress. The best results were obtained with 0.6 s/cm plasma and 0.06 M CuO NPs, maximizing germination, vigor, and seedling growth without toxicity, demonstrating their potential as practical tools for improving rice seed quality and pathogen management. Full article

Microbial contamination is the main safety concern of sprouts and seeds are the major source. High concentrations of sanitizers (>10,000 mg/kg) are recommended for effective sanitation. Microbubble (MB) was reported to elevate sanitizer efficacy. Hence, MBs combined with disinfectants, chlorine dioxide (ClO₂, 500 ppm), and slightly acidic electrolyzed water (SAEW, 250 ppm), were used to inactivate Salmonella Typhimurium on alfalfa seeds. After fulfilling MBs for 10 min, alfalfa seeds were washed in 10 L of water for 10, 20, or 30 min. Compared with untreated seeds, S. Typhimurium reductions obtained by SAEW-MBs (SMBs) and ClO₂-MBs (CMBs) for 20 min were 3.8 and 3.3 log CFU/g, respectively. Conversely, the 20 min treatments of SAEW and ClO₂ only obtained reductions of 0.9 and 1.1 log CFU/g, respectively. More surface ruptures on the seeds treated with CMBs were observed under a scanning electron microscope compared with the ones treated by water and ClO₂ only. No adverse effects on the seed germination rate and the weight yield of sprouts were observed when treated with CMBs for 20 min. An MB device with capacity of 100 L was assembled and achieved reductions of 3.9 and 3.2 log CFU/g of natural microbes and S. Typhimurium, respectively, after 20 min CMB washing. Additionally, an MB device at 250 L was assembled and achieved 3.0 log CFU/g reduction in natural microbes. This study demonstrated that MBs enhanced the efficacy of disinfectants and could be applied in industrial-scale operations. Full article

Corylus avellana L. is a rare and endangered species in Kazakhstan, included in the national Red Book. The results of morphological and genetic characterization of the sole known natural population of C. avellana in the Western Kazakhstan region are presented in this study. Sixty wild accessions were evaluated based on tree and leaf morphological traits using standard descriptors in accordance with Bioversity International guidelines. Genetic diversity was assessed using ten nuclear simple sequence repeat (SSR) markers. A total of 120 alleles were detected across the nuclear loci, with the number of alleles per locus ranging from 9 to 16 and an average of 12. The mean effective number of alleles (Ne) per locus was 3.862. A high level of intraspecific polymorphism was observed, with an average observed heterozygosity (Ho) of 0.70. The population showed considerable genetic diversity, as highlighted by a mean Shannon’s diversity index of 1.526. STRUCTURE, PCoA, and phylogenetic analyses confirmed strong differentiation between the wild Kazakh population and the cultivated hazelnut germplasm. Due to the lack of viable seeds, in vitro conservation was initiated using vegetative shoots. A two-step disinfection protocol, involving Plant Preservative Mixture and mercuric chloride, significantly improved explant survival, enabling successful establishment of an aseptic in vitro collection. These findings highlight the urgent need for targeted conservation strategies and show the potential of biotechnological approaches for safeguarding Kazakhstan’s only natural C. avellana population. Full article

This study presents the development and evaluation of an automated ultraviolet-C irradiation system for maize seed treatment, emphasizing disinfection performance, environmental control, and vision-based monitoring. The system features dual 8-watt ultraviolet-C lamps, sensors for temperature and humidity, and an air extraction unit to regulate the microclimate of the chamber. Without air extraction, radiation stabilized within one minute, with internal temperatures increasing by 5.1 °C and humidity decreasing by 13.26% over 10 min. When activated, the extractor reduced heat build-up by 1.4 °C, minimized humidity fluctuations (4.6%), and removed odors, although it also attenuated the intensity of ultraviolet-C by up to 19.59%. A 10 min ultraviolet-C treatment significantly reduced the fungal infestation in maize seeds by 23.5–26.25% under both extraction conditions. Thermal imaging confirmed localized heating on seed surfaces, which stressed the importance of temperature regulation during exposure. Notable color changes ($\Delta E>2.3$) in treated seeds suggested radiation-induced pigment degradation. Ultraviolet-C intensity mapping revealed spatial non-uniformity, with measurements limited to a central axis, indicating the need for comprehensive spatial analysis. The integrated computer vision system successfully detected seed contours and color changes under high-contrast conditions, but underperformed under low-light or uneven illumination. These limitations highlight the need for improved image processing and consistent lighting to ensure accurate monitoring. Overall, the chamber shows strong potential as a non-chemical seed disinfection tool. Future research will focus on improving radiation uniformity, assessing effects on germination and plant growth, and advancing system calibration, safety mechanisms, and remote control capabilities. Full article

In vitro micropropagation is used to rapidly shorten the breeding process of crops, such as kale, an internationally widespread winter vegetable. The aim of this study is to develop optimised micropropagation protocols for three kale varieties. First, it was determined which seed surface disinfection method resulted in the highest germination rate and the lowest infection rate. Secondly, it was investigated which of several existing Brassica protocols and one modified protocol from the literature provided the highest regeneration efficiency of kale explant types (cotyledons, hypocotyl, root, and intact seedlings as the control) after eight weeks of cultivation. Germination was highest and fastest after disinfection with 10% NaClO for 10 min for “Frostara” and at 5% for 2.5 min for “Schatteburg”. The infection rate and speed were lowest in treatments with 10% NaClO. The regeneration efficiency and number of newly formed leaves, roots, shoots, and stems varied between media, explant type, and kale variety. Most new leaves and shoots were formed when hypocotyls were used as explant type. Roots regenerated mostly more roots than shoots, stems, and leaves. A higher ratio of auxin to cytokinin in the culture medium partially increased leaf regeneration. The addition of AgNO₃ increased shoot regeneration and reduced yellowing and leaf drop. Phenotypic anomalies occurred less frequently in media with lower hormone concentrations. All tested protocols are suitable for kale micropropagation, but regeneration was highly dependent on the medium for different varieties and explant types. Therefore, this study builds a basis for future micropropagation of kale and the development of variety-specific protocols for maximum commercial success. Full article

Asparagus decline syndrome (ADS) poses a significant threat to asparagus cultivation worldwide. To address this challenge, a two-year investigation was carried out in Spain to assess the impacts of three soil disinfection strategies on asparagus crops. These included biofumigation with Brassica carinata seed pellets, biofumigation using poultry manure pellets, and chemical disinfection with dazomet. In addition to evaluating the potential of these treatments to alleviate ADS, the research also focused on identifying the physiological changes linked to the syndrome by examining indicators of oxidative metabolism, hormonal equilibrium, and phenolic compound profiles. Among the treatments evaluated, biofumigation with B. carinata pellets enhanced vegetative growth, photosynthetic pigment accumulation, antioxidant capacity, and hormonal homeostasis, with these improvements becoming more pronounced in the second year. This approach appeared to promote a healthier physiological status in asparagus plants, likely through improved soil health and reduced biotic and abiotic stress perception. In contrast, chemical disinfection with dazomet, despite initially stimulating some physiological responses, was associated with elevated oxidative stress. Overall, the findings suggest that organic-based soil treatments, particularly B. carinata biofumigation, represent a promising strategy to strengthen asparagus vigor and resilience against ADS. Further studies are needed to assess their long-term effects in perennial cultivation systems. Full article

Pomegranates are rich in nutrients and classified among ready-to-eat fruits and vegetables. Although this ready-to-eat produce offers convenience, it presents risks associated with pathogenic microorganisms, highlighting the need for pre-sale disinfection. Ultraviolet light-emitting diodes (UV-LEDs) constitute an innovative non-thermal processing technology for food products, offering reduced heat generation and lower energy consumption compared to traditional ultraviolet (UV) irradiation methods. This study analyzed the effects of UV-LED technology on pomegranate seed quality over 0 to 5 days of storage. The results demonstrated significant increases in anthocyanins, polyphenols, ascorbic acid, and the antioxidant capacity in pomegranate following treatment, peaking on day 3. In contrast, the control group showed declining trends. After treatment, the aerobic mesophilic counts and counts of mold and yeast levels during storage measured between 2.73–3.23 log CFU/g and 2.56–3.29 log CFU/g, respectively, significantly lower than the control group. Non-targeted metabolomic analysis showed that UV-LED treatment prompted modifications in the biosynthetic pathways of flavonoids, flavonols, and anthocyanins. The expression of peonidin-3-O-rutinoside chloride increased by 46.46-fold within the anthocyanin biosynthesis pathway. In conclusion, UV-LED treatment represents a potential approach to the disinfection of ready-to-eat fruits and vegetables. Full article