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Keywords = rotating structures

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24 pages, 1196 KB  
Article
Long-Term Straw Return Combined with Chemical Fertilizer Enhances Crop Yields in Wheat-Maize Rotation Systems by Improving Soil Nutrients Stoichiometry and Aggregate Stability in the Shajiang Black Soil (Vertisol) Region of North China Plain
by Xian Tang, Yangfan Qu, Yu Wu, Shasha Li, Fuwei Wang, Dongxue Li, Xiaoliang Li, Jianfei Wang and Jianrong Zhao
Agronomy 2025, 15(12), 2861; https://doi.org/10.3390/agronomy15122861 - 12 Dec 2025
Abstract
The sustainability of wheat-maize rotation systems in the North China Plain is challenged by the over-reliance on chemical fertilizers, which leads to the decline of soil organic matter and structural degradation, particularly in the unique Shajiang black soil (Vertisol). While straw return is [...] Read more.
The sustainability of wheat-maize rotation systems in the North China Plain is challenged by the over-reliance on chemical fertilizers, which leads to the decline of soil organic matter and structural degradation, particularly in the unique Shajiang black soil (Vertisol). While straw return is widely recommended to mitigate these issues, the synergistic mechanisms of its long-term combination with chemical fertilizers on soil nutrient stoichiometry and aggregate stability remain inadequately quantified. A long-term field experiment was conducted with the five fertilization treatments including: (1) no fertilizer or straw (CK), (2) chemical fertilizer alone (NPK), (3) straw return chemical fertilizer (NPKS), (4) straw return with 10% straw-decomposing microbial inoculant combined with chemical fertilizer (10%NPKS), and (5) straw return with 20% straw-decomposing microbial inoculant combined with chemical fertilizer (20%NPKS) in the Shajiang black soil (Vertisol) region to investigate the effects of straw return combined with chemical fertilizers on soil organic carbon (SOC), total nitrogen (TN) and total phosphorus (TP) stoichiometry, aggregate stability, and crop yield in winter wheat-summer maize rotation systems of North China Plain. Our study demonstrated that the co-application of straw with a straw-decomposing microbial inoculant is a highly effective strategy for enhancing soil health and crop productivity, with its efficacy being critically dose-dependent. Our results identified the 10%NPKS treatment as the optimal practice. It most effectively improved soil physical structure by significantly increasing the content of large macroaggregates (>0.5 mm) and key stability indices (MWD, GMD, WA), while concurrently enhancing nutrient cycling, as evidenced by elevated SOC, TN, and shifted C/P and N/P stoichiometry. Multivariate analyses confirmed strong positive correlations among these soil properties, indicating a synergistic improvement in soil quality. Crucially, these enhancements translated into significant yield gains, with a notable crop-specific response: maize yield was maximized under the 10%NPKS treatment, whereas wheat yield benefited sufficiently from NPKS treatment. A key mechanistic insight was that 20%NPKS treatment, despite leading to the highest SOC and TN, induced a relative phosphorus limitation and likely caused transient nutrient immobilization, thereby attenuating its benefits for soil structure and yield. We conclude that co-applying straw with a 10% microbial inoculant combined with chemical fertilizer represents the superior strategy, offering a sustainable pathway to synergistically improve soil structure, nutrient availability, and crop productivity, particularly in maize-dominated systems. Full article
(This article belongs to the Special Issue Plant Nutrition Eco-Physiology and Nutrient Management)
19 pages, 2104 KB  
Article
Design and Experiment of Axial Flow Threshing and Cleaning Device for Roller Brush Type Castor Harvesting Machine
by Teng Wu, Bin Zhang, Fanting Kong, Yongfei Sun, Qing Xie, Huayang Zhao and Shuhe Zheng
Agriculture 2025, 15(24), 2578; https://doi.org/10.3390/agriculture15242578 - 12 Dec 2025
Abstract
In order to alleviate the problems of lack of research on threshing and cleaning equipment and poor operational performance of castor harvester, an axial-flow threshing and cleaning device was designed and evaluated for a roller brush type castor harvester. This paper introduces the [...] Read more.
In order to alleviate the problems of lack of research on threshing and cleaning equipment and poor operational performance of castor harvester, an axial-flow threshing and cleaning device was designed and evaluated for a roller brush type castor harvester. This paper introduces the overall machine structure and elaborates on the working principles of the castor threshing and cleaning device. It clarifies the design and analysis of key components such as the conveyor design, rod-tooth structure design, collision force analysis between the fruit and rod-tooth, concave sieve design, and guide plate design. The main indicators for evaluating the castor threshing and cleaning device include the impurity rate, damage rate, and separation loss rate. Based on the previous experimental research, the working parameters of castor threshing and cleaning device are tested and studied by using the Box–Behnken central combined test method. The three-factor three-level quadratic regression orthogonal test design is carried out based on the forward speed, roller rotational speed, and threshing gap of concave sieve. A response surface mathematical model was established, analyzing the impact of various factors on work quality and conducting comprehensive optimization of influencing factors. The experimental results indicate that the significance order of factors affecting the impurity rate was forward speed > roller rotational speed > threshing gap of concave sieve; the significance order for damage rate was roller rotational speed > threshing gap of concave sieve > forward speed; and the significance order for separation loss rate was roller rotational speed > forward speed > threshing gap of concave sieve. The field test results show that the optimal working parameter combination is forward speed of 0.87 m∙s−1, roller rotational speed of 462 r∙min−1, and threshing gap of concave sieve of 30 mm, with an impurity rate of 2.95%, a damage rate of 1.75%, and a separation loss rate of 0.49%. The research findings can provide references for the structural improvement and operational parameter optimization of the castor harvester’s threshing and cleaning device. Full article
(This article belongs to the Section Agricultural Technology)
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28 pages, 11120 KB  
Article
Behavior of Nonconforming Flexure-Controlled RC Structural Walls Under Reversed Cyclic Lateral Loading
by Yusuf Şahinkaya, Ergün Binbir, Kutay Orakçal and Alper İlki
Buildings 2025, 15(24), 4501; https://doi.org/10.3390/buildings15244501 - 12 Dec 2025
Abstract
Reinforced concrete (RC) structural walls are essential for ensuring adequate lateral stiffness and strength in buildings located in seismic regions. However, many older structures incorporate nonconforming walls constructed with low-strength concrete, plain longitudinal reinforcement, and insufficient boundary confinement, and experimental data on such [...] Read more.
Reinforced concrete (RC) structural walls are essential for ensuring adequate lateral stiffness and strength in buildings located in seismic regions. However, many older structures incorporate nonconforming walls constructed with low-strength concrete, plain longitudinal reinforcement, and insufficient boundary confinement, and experimental data on such systems remain limited. This study investigates the seismic performance of two full-scale, relatively slender nonconforming RC wall specimens representative of older construction: one with no boundary confinement (SW-NC-FF) and one with insufficient confinement (SW-IC-FF). Both specimens exhibited flexure-controlled behavior, with initial yielding of boundary longitudinal bars occurring at an approximately 0.30% drift ratio and maximum reinforcement tensile strains of 0.006 (SW-IC-FF) and 0.015 (SW-NC-FF). Rocking governed the lateral response due to progressive debonding of the plain bars along the wall height, producing pronounced pinching and self-centering behavior. Failure occurred through longitudinal bar buckling and concrete crushing, with ultimate drift ratios of 2.0% and 1.5% and displacement ductility values of 4.0 and 4.3 for SW-IC-FF and SW-NC-FF, respectively. Experimental results were compared with backbone predictions from ASCE 41:2023, NZ C5:2025, and EN 1998-3:2025. While all three guidelines captured initial stiffness and yield rotations, their rotation-capacity predictions diverged, underscoring the need for improved assessment approaches for rocking-dominated, plain-reinforced walls. Full article
(This article belongs to the Section Building Structures)
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30 pages, 4710 KB  
Article
Island Community-Based Tourism and Gendered Power: How Respectability and Paperwork Organize Women’s Everyday Authority in Phuket, Thailand
by Siyuan Liu and Piya Pangsapa
Tour. Hosp. 2025, 6(5), 281; https://doi.org/10.3390/tourhosp6050281 - 12 Dec 2025
Abstract
This study investigates how women at a Muslim island community-based tourism (CBT) site convert performed respectability and routine paperwork into everyday organizational authority. Drawing on four months of ethnographic fieldwork in Bang Rong, Phuket—supported by seventeen semi-structured interviews, three years of social-media observation [...] Read more.
This study investigates how women at a Muslim island community-based tourism (CBT) site convert performed respectability and routine paperwork into everyday organizational authority. Drawing on four months of ethnographic fieldwork in Bang Rong, Phuket—supported by seventeen semi-structured interviews, three years of social-media observation (2023–2025), and analysis of rosters, ledgers, receipts, and LINE threads—the study examines how gendered norms and material devices structure authority in daily tourism practice. The analysis identifies an authorization stack (veil, uniform, tone) and a set of paperwork devices (ledgers, rosters, receipts, digital groups) that make women’s visibility both morally credible and institutionally legible. Using a poststructural feminist lens and Barriteau’s gender-system framework, the article develops an interpretive, case-derived Respectability-in-Action Conversion Model, showing that moral credit converts into procedural authority only when respectability cues align with control of at least one device. Conversion, however, remains partial and contingent: strategic levers stay largely male or mosque-adjacent unless women obtain rights-bearing tools, such as co-signature authority, petty-cash control, or platform access, along with institutional protection against sanction. Age, class, and endorsement shape these trajectories, enabling some women to consolidate authority while rendering others easily replaceable. The study contributes: (1) a case-specific, empirically grounded account of authority formation in island CBT; (2) an analytic lens for understanding how performance, devices, and rights interact in this setting; and (3) practice-oriented implications for small-island CBT contexts that emphasize shared device access, rotating administrative duties, co-signature and budget rights, and safeguards against organizational capture. Full article
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43 pages, 6486 KB  
Review
Instrumentation Strategies for Monitoring Flow in Centrifugal Compressor Diffusers: Techniques and Case Studies
by Emilia-Georgiana Prisăcariu and Oana Dumitrescu
Sensors 2025, 25(24), 7526; https://doi.org/10.3390/s25247526 - 11 Dec 2025
Abstract
Monitoring the complex, three-dimensional flow within centrifugal compressor diffusers remains a major challenge due to geometric confinement, high rotational speeds, and strong unsteadiness near surge and stall. This review provides a comprehensive assessment of contemporary instrumentation strategies for diffuser flow characterization, spanning pressure, [...] Read more.
Monitoring the complex, three-dimensional flow within centrifugal compressor diffusers remains a major challenge due to geometric confinement, high rotational speeds, and strong unsteadiness near surge and stall. This review provides a comprehensive assessment of contemporary instrumentation strategies for diffuser flow characterization, spanning pressure, temperature, velocity, vibration, and acoustic measurements. The article outlines the standards governing compressor instrumentation, compares conventional probes with emerging high-resolution and high-bandwidth sensor technologies, and evaluates the effectiveness of pressure- and temperature-based diagnostics, optical methods, and advanced dynamic sensing in capturing diffuser behavior. Case studies from industrial compressors, research rigs, and high-speed experimental facilities illustrate how sensor layout, bandwidth, and synchronization influence the interpretation of flow stability, performance degradation, and surge onset. Collectively, these examples demonstrate that high-frequency pressure and temperature probes remain indispensable for instability detection, while optical techniques such as PIV, LDV, and PSP/TSP offer unprecedented spatial resolution for understanding flow structures. The findings highlight the growing integration of hybrid sensing architectures, digital acquisition systems, and data-driven analysis in diffuser research. Overall, the review identifies current limitations in measurement fidelity and accessibility while outlining promising paths toward more robust, real-time monitoring solutions for reliable centrifugal compressor operation. Full article
(This article belongs to the Section Physical Sensors)
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26 pages, 3148 KB  
Article
Experimental Evaluation of Pulsating and Rotating HFI Methods with Adaptive-Gain SMO for Sensorless IPM Compressor Drives
by Tunahan Sapmaz and Ahmet Faruk Bakan
World Electr. Veh. J. 2025, 16(12), 669; https://doi.org/10.3390/wevj16120669 - 11 Dec 2025
Abstract
This paper presents a comprehensive sensorless control approach for interior permanent magnet (IPM) motors, integrating high-frequency injection (HFI) and model-based observer techniques to ensure accurate rotor position estimation across a wide speed range. Two HFI strategies—pulsating and rotating—are investigated experimentally and compared in [...] Read more.
This paper presents a comprehensive sensorless control approach for interior permanent magnet (IPM) motors, integrating high-frequency injection (HFI) and model-based observer techniques to ensure accurate rotor position estimation across a wide speed range. Two HFI strategies—pulsating and rotating—are investigated experimentally and compared in combination with two observer structures: the conventional Sliding Mode Observer (SMO) and Adaptive-Gain SMO (AG-SMO). The AG-SMO dynamically adjusts its observer gain according to the estimated back-electromotive force (back-EMF) amplitude, significantly reducing chattering and improving estimation performance under varying load and noise conditions. A Frequency-Adaptive Complex Coefficient Filter (FACCF) and an Orthogonal Phase-Locked Loop (PLL) are incorporated to eliminate phase delay and enhance demodulation accuracy. Simulation and experimental results obtained using a 30 W, 20 V IPM motor demonstrate that the pulsating HFI + AG-SMO configuration achieves superior stability and noise immunity, while the rotating HFI + AG-SMO provides smoother and more accurate estimation. Overall, the proposed hybrid control framework achieves robust, high-precision, and sensorless operation for IPM motors over the wide speed range, offering a practical solution for applications such as inverter-driven compressor systems operating in noisy environments. Full article
(This article belongs to the Section Propulsion Systems and Components)
13 pages, 3501 KB  
Article
Channel-Free Micro-Well–Template-Assisted Magnetic Particle Trapping for Efficient Single-Particle Isolation
by Jin-Yeong Park, Kyeong-Taek Nam, Young-Ho Nam, Yong-Kweon Kim, Seung-Ki Lee and Jae-Hyoung Park
Micromachines 2025, 16(12), 1397; https://doi.org/10.3390/mi16121397 - 11 Dec 2025
Abstract
This study presents a channel-free, micro-well–template-assisted magnetic particle trapping method for efficient single-particle isolation without the need for microfluidic channels. Dual-surface silicon micro-well arrays were fabricated using photolithography, PE-CVD, and DRIE processes, featuring hydrophilic well interiors and hydrophobic outer surfaces to enhance trapping [...] Read more.
This study presents a channel-free, micro-well–template-assisted magnetic particle trapping method for efficient single-particle isolation without the need for microfluidic channels. Dual-surface silicon micro-well arrays were fabricated using photolithography, PE-CVD, and DRIE processes, featuring hydrophilic well interiors and hydrophobic outer surfaces to enhance trapping performance. The proposed method combines magnet-assisted sedimentation with rotational sweeping of a glass slide placed above the micro-well array, enabling rapid and uniform particle confinement within a 250 × 250 well array. Experimental results showed that the trapping efficiency increased with the well width and depth, achieving over 93.8% within three trapping cycles for optimized structures. High single-particle occupancy was obtained for wells of comparable size to the particle diameter, while deeper wells enabled stable trapping with minimal loss. The entire trapping process was completed within five minutes per cycle, demonstrating a rapid, simple, and scalable approach applicable to digital immunoassay systems for ultrasensitive biomolecule detection. Full article
(This article belongs to the Special Issue Microfluidics in Biomedical Research)
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24 pages, 4686 KB  
Article
Parameter Calibration and Experimentation of the Discrete Element Model for Mixed Seeds of Vetch (Vicia villosa) and Oat (Avena sativa) in a Pneumatic Seed Drilling System
by Yu Fu, Dewei Wang, Xufeng Wang, Long Wang, Jianliang Hu, Xingguang Chi and Mao Ji
Appl. Sci. 2025, 15(24), 13048; https://doi.org/10.3390/app152413048 - 11 Dec 2025
Viewed by 12
Abstract
This paper focuses on mixed seeds of Vicia villosa and Avena sativa, with their discrete element model and contact parameters being systematically calibrated and validated to provide reliable theoretical support for the structural design and parameter optimization of the air-assisted seed delivery [...] Read more.
This paper focuses on mixed seeds of Vicia villosa and Avena sativa, with their discrete element model and contact parameters being systematically calibrated and validated to provide reliable theoretical support for the structural design and parameter optimization of the air-assisted seed delivery system. The physical properties of both seed types, including triaxial dimensions, density, moisture content, Poisson’s ratio, and shear modulus, were first measured. The Hertz–Mindlin (no slip) contact model and the multi-sphere aggregation method were employed to construct the discrete element models of Vicia villosa and Avena sativa, with preliminary calibration of the intrinsic model parameters. Poisson’s ratio, elastic modulus, collision restitution coefficient, static friction coefficient, and rolling friction coefficient between the seeds and PLA plastic plate were determined through uniaxial compression, free fall, inclined sliding, and inclined rolling tests. Each test was repeated five times, and the calibration criterion for contact parameters was based on minimizing the relative error between simulation and experimental results. Based on this, experiments on the packing angle of mixed seeds, steepest slope, and a three-factor quadratic rotational orthogonal combination were conducted. The inter-seed collision restitution coefficient, static friction coefficient, and rolling friction coefficient were set as the experimental factors. A total of 23 treatments were designed with repetitions at the center point, and a regression model was established for the relative error of the packing angle with respect to each factor. Based on the measured packing angle of 28.01° for the mixed seeds, the optimal contact parameter combination for the mixed seed pile was determined to be: inter-seed collision restitution coefficient of 0.312, static friction coefficient of 0.328, and rolling friction coefficient of 0.032. The relative error between the simulated packing angle and the measured value was 1.32%. The calibrated inter-seed contact parameters were further coupled into the EDEM–Fluent gas–solid two-phase flow model. Simulations and bench verification tests were carried out under nine treatment combinations, corresponding to three fan speeds (20, 25, and 30 m·s−1) and three total transport efficiencies (12.5, 17.5, and 22.5 g·s−1), with the consistency coefficient of seed distribution in each row being the main evaluation variable. The results showed that the deviation in the consistency coefficient of seed distribution between the simulation and experimental measurements ranged from 1.24% to 3.94%. This indicates that the calibrated discrete element model for mixed seeds and the EDEM–Fluent coupled simulation can effectively reproduce the air-assisted seed delivery process under the conditions of Vicia villosa and Avena sativa mixed sowing, providing reliable parameters and methodological support for the structural design of seeders and DEM-CFD coupled simulations in legume–grass mixed sowing systems. Full article
(This article belongs to the Section Agricultural Science and Technology)
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18 pages, 21101 KB  
Article
Effect of Fabric Topology and Axial Yarn Condition on the Compressive Properties of 3D Stepwise Rotary Braided Composites
by Haiyang Mei, Long Sun, Ran Yang, Qian Zhang, Yankuo Guo and Zhenyu Han
Materials 2025, 18(24), 5561; https://doi.org/10.3390/ma18245561 - 11 Dec 2025
Viewed by 17
Abstract
Three-dimensional braided composites have become one kind of critical engineering material for applications in extreme environments. The 3D stepwise rotary braiding process is one vital technique for manufacturing preforms with high efficiency and flexibility. However, the fabric topology is decided by the combination [...] Read more.
Three-dimensional braided composites have become one kind of critical engineering material for applications in extreme environments. The 3D stepwise rotary braiding process is one vital technique for manufacturing preforms with high efficiency and flexibility. However, the fabric topology is decided by the combination of switch rotation directions, which affects the mechanical properties, and the full carrier configuration results in a loose four-directional structure which is supposed to be improved by adding axial yarns. Therefore, experiments are carried out to illustrate the effect of fabric topology and axial yarn condition on the compressive properties of 3D stepwise rotary braided composites. Samples with three types of fabric topologies named Type A, B, and C are prepared under four axial yarn conditions including no axial yarn addition, 12K axial yarn addition, 24K axial yarn addition, and 36K axial yarn addition, which are fabricated with braiding angles of 20°, 30° and 40°. Longitudinal and transverse compression tests are conducted, and the morphology is observed. It shows that the braiding angle has more influence on the longitudinal compressive properties than transverse compressive properties, and the effect of fabric topology and axial yarn condition depends on the braiding angle. The fabric topology affects a lot on the longitudinal compressive properties when the braiding angle is small, resulting in a gap of up to 40%. The longitudinal compressive properties are improved significantly by adding axial yarns especially for the composites with large braiding angles, making the strength more than double. With the increase in axial yarn size, the strength increment gradually decreases while the modulus declines after a certain size for smaller braiding angles. Full article
(This article belongs to the Section Advanced Composites)
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23 pages, 4348 KB  
Article
Acute Decrease in Glenohumeral Internal Rotation During Repetitive Baseball Pitching Is Associated with Transient Structural Changes in Medial Longitudinal Arch of Stride Leg: Pilot Study Using Mixed Model
by Takeru Abekura, Noriaki Maeda, Tsubasa Tashiro, Satoshi Arima, Ryosuke Kaizuka, Madoka Koyanagi, Koshi Iwata, Haruka Yoshida, Ginji Ito, Mayu Ueda and Takashi Yamada
Sports 2025, 13(12), 446; https://doi.org/10.3390/sports13120446 - 10 Dec 2025
Viewed by 70
Abstract
Pitching requires effective transfer of ground reaction force (GRF), and structural breakdown of the medial longitudinal arch (MLA) may influence glenohumeral internal rotation (IR) deficits. This study investigated whether changes in foot morphology of the stride leg and soft tissue characteristics are associated [...] Read more.
Pitching requires effective transfer of ground reaction force (GRF), and structural breakdown of the medial longitudinal arch (MLA) may influence glenohumeral internal rotation (IR) deficits. This study investigated whether changes in foot morphology of the stride leg and soft tissue characteristics are associated with loss of IR during repeated pitching. Fifteen male college pitchers completed 60 pitches in a simulated game. IR range of motion (IRROM) was assessed before and after pitching. The navicular height, mechanical properties of the abductor hallucis (AbH) and plantar fascia, and GRF were measured at multiple time points. Correlation analysis and a linear mixed model were used to identify predictors of IRROM change. The mean change in shoulder IRROM during pitching was −21.9° ± 8.4°. IRROM and navicular height decreased significantly over time. The AbH elasticity increased throughout the pitching sequence. Greater reductions in IRROM appeared related to a higher vertical GRF (p = 0.021) and increased AbH elasticity (p = 0.046). Vertical GRF was unrelated to fastball velocity (p = 0.260), whereas anteroposterior GRF correlated with fastball velocity (p = 0.038). Morphological and mechanical changes in the stride leg, particularly within the support of the MLA, can influence IRROM. Reducing vertical GRF and stress on the AbH may help preserve the IRROM without compromising performance. Full article
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28 pages, 82749 KB  
Article
Degradation and Decay of Rocks: Linking Wetting–Drying and Slake Durability Tests for Climate-Sensitive Maintenance
by Markus Kaspar, Christine Latal, Gerhard Pittino and Volker Reinprecht
Geotechnics 2025, 5(4), 84; https://doi.org/10.3390/geotechnics5040084 - 10 Dec 2025
Viewed by 75
Abstract
Soft and weak rocks present challenges for construction activities in various environments. Their genetic origin, geological and tectonic evolution, and exposure to atmospheric conditions control their weathering and degradation over time. Therefore, a sound characterization of the associated rock parameters is essential. Numerous [...] Read more.
Soft and weak rocks present challenges for construction activities in various environments. Their genetic origin, geological and tectonic evolution, and exposure to atmospheric conditions control their weathering and degradation over time. Therefore, a sound characterization of the associated rock parameters is essential. Numerous tests have been developed and standardized or defined in recommendations to assess various geomechanical, petrological, and mineralogical parameters. However, these tests are still subject to modification or extension to address project-specific issues. Additionally, standardized tests do not consider regional climatic conditions that may affect weathering, meaning they do not reflect the degradation behavior that is observed in the field. The present study investigates the slaking resistance and degradability of a range of soft rocks. The workflow of widely used tests is employed to evaluate their representativeness for different rock types in practical applications. Depending on their genetic origin and mineral composition, fabric alterations affect the rate and style of rock disintegration differently. Soft sedimentary rocks react already to static slaking, i.e., water immersion, whereas crystalline and grain-bound rocks slake under dynamic action while undergoing attrition in a rotating slake durability drum. Zones of structural weakness, such as foliation planes, are responsible for material removal in the latter; sedimentary rocks, on the other hand, are subject to surface particle separation (suspension) and suction due to the presence of clay minerals. This study presents an approach that combines the results of several routine tests to help identify and refine the slaking susceptibility of different rock types. A routine for inspecting and documenting the evaluated slaking characteristics for infrastructure maintenance is proposed, and the wider implications in light of climate change are discussed. Some limitations of the transferability of laboratory values to field sites still have to be evaluated and validated in the future. Full article
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14 pages, 2619 KB  
Article
A Stable Tetraphenylethylene-Based Charge-Assisted Hydrogen-Bonded Organic Framework for Turn-On Fluorescence Sensing of Al3+ Ions
by Yingjia Deng, Yijin Wang, Xiangyu Gao, Yunke Jin, Jiabao Liu, Guanglai Mo, Yixuan Guo, Lanlu Lu and Peng Li
Molecules 2025, 30(24), 4725; https://doi.org/10.3390/molecules30244725 - 10 Dec 2025
Viewed by 140
Abstract
The development of stable and sensitive fluorescent sensors for metal ion detection remains a challenge in materials chemistry. Although hydrogen-bonded organic frameworks (HOFs) have shown great potential in luminescent applications, their practical use is often limited by structural instability. In this work, we [...] Read more.
The development of stable and sensitive fluorescent sensors for metal ion detection remains a challenge in materials chemistry. Although hydrogen-bonded organic frameworks (HOFs) have shown great potential in luminescent applications, their practical use is often limited by structural instability. In this work, we present a novel charge-assisted HOF, termed FDU-HOF-21 ([H(NH2Bpy)]2(TPE)), constructed from a tetraphenylethylene (TPE)-based carboxylic acid ligand (H4TCPE) and 2,2′-bipyridine-5,5′-diamine (NH2Bpy). Single-crystal X-ray diffraction (SCXRD) reveals a stable three-dimensional framework stabilized by an extensive hydrogen-bonding network and reinforced by charge-assisted hydrogen bonds (CAHBs), and it exhibits exceptional stability across various solvents and pH conditions. Moreover, FDU-HOF-21 serves as a highly sensitive and selective fluorescent turn-on sensor for Al3+ ions, with a lowest limit of detection (LOD) of 1.7 × 10−6 M. Characterization and time-dependent density functional theory (TDDFT) calculations reveal that the fluorescence enhancement originates from the suppression of non-radiative decay likely due to the reduction in intermolecular charge transfer (Inter-CT) during the emission process, coupled with the restricted intramolecular rotation upon Al3+ chelation. Full article
(This article belongs to the Special Issue Design and Application of Hydrogen-Bonded Organic Frameworks (HOFs))
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20 pages, 5981 KB  
Article
A Multimodal Visual–Textual Framework for Detection and Counting of Diseased Trees Caused by Invasive Species in Complex Forest Scenes
by Rui Zhang, Zhibo Chen, Guangyu Huo, Xiaoyu Zhang, Wenda Luo and Liping Mu
Remote Sens. 2025, 17(24), 3971; https://doi.org/10.3390/rs17243971 - 9 Dec 2025
Viewed by 123
Abstract
With the large-scale invasion of alien species, forest ecosystems are facing severe challenges, and the health of trees is increasingly threatened. Accurately detecting and counting trees affected by such invasive species has become a critical issue in forest conservation and resource management. Traditional [...] Read more.
With the large-scale invasion of alien species, forest ecosystems are facing severe challenges, and the health of trees is increasingly threatened. Accurately detecting and counting trees affected by such invasive species has become a critical issue in forest conservation and resource management. Traditional detection methods usually rely only on the information of a single modality of an image, lack linguistic or semantic guidance, and often can only model a specific diseased tree situation during training, making it difficult to achieve effective differentiation and generalization of multiple diseased tree types, which limits their practicality. To address the above challenges, we propose an end-to-end multimodal diseased tree detection model. In the visual encoder of the model, we introduce rotational positional encoding to enhance the model’s ability to perceive detailed structures of trees in images. This design enables more accurate extraction of features related to diseased trees, especially when processing images with complex environments. At the same time, we further introduce a cross-attention mechanism between image and text modalities, so that the model can realize the deep fusion of visual and verbal information, thus improving the detection accuracy based on understanding and recognizing the semantics of the disease. Additionally, this method possesses strong generalization capabilities, enabling effective recognition based on textual descriptions even when samples are not available. Our model achieves optimal results on the Larch Casebearer dataset and the Pests and Diseases Tree dataset, verifying the effectiveness and generalizability of the method. Full article
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27 pages, 964 KB  
Article
Prematurity and Multidimensional Risk Patterns in Adolescent and Adult Pregnancies: A Principal Component Analysis in an Eastern European Cohort
by Florin Tovirnac, Alina Mihaela Calin, Catalin Plesea-Condratovici, Monica-Laura Zlati and Nicoleta Andreea Tovirnac
Children 2025, 12(12), 1673; https://doi.org/10.3390/children12121673 - 9 Dec 2025
Viewed by 129
Abstract
Background: Preterm birth remains a major cause of neonatal morbidity and mortality, with risk shaped by interacting maternal, fetal, placental and behavioural factors. This study examined latent multidimensional risk patterns in adolescent and adult pregnancies in an Eastern European cohort. Methods: We conducted [...] Read more.
Background: Preterm birth remains a major cause of neonatal morbidity and mortality, with risk shaped by interacting maternal, fetal, placental and behavioural factors. This study examined latent multidimensional risk patterns in adolescent and adult pregnancies in an Eastern European cohort. Methods: We conducted a retrospective observational study including all non-COVID pregnant women who delivered at the County Emergency Clinical Hospital of Brăila, Romania, between 2020 and 2021. Three cohorts were analyzed: adolescent preterm mothers (Lot E; n = 54), adult preterm mothers (Lot P; n = 231) and adult term mothers (Lot M; n = 3354). Maternal, fetal, placental and behavioural indicators were coded as ordered clinical risk categories, and separate principal component analyses (PCA) with Varimax rotation were performed within each cohort. Results: Across all three groups, PCA identified three latent dimensions that together explained approximately 66–72% of the total variance. The composition of these components differed by cohort: in adolescents, maternal complications, exogenous behaviours and obstetric–placental indicators tended to cluster; in adult preterm pregnancies, placental–obstetric and behavioural indicators formed distinct but interrelated dimensions; and in adult term pregnancies, behavioural and socio-environmental indicators were the most prominent contributors to the latent structure, with fetal outcomes forming a separate dimension. Conclusions: Prematurity-related risk profiles were multidimensional and varied meaningfully by age and pregnancy outcome. These exploratory PCA-derived dimensions provide a data-driven framework for understanding how risk clusters across different maternal populations and may help generate hypotheses for age-specific preventive and clinical strategies. Confirmation and further validation in prospective, multicentre studies are required before clinical application. Full article
(This article belongs to the Section Pediatric Neonatology)
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Article
Assessment of Collagen and Fibroblast Properties via Label-Free Higher Harmonic Generation Microscopy in Three-Dimensional Models of Osteogenesis Imperfecta and Ehlers-Danlos Syndrome
by Yuanyuan Ma, Qiyu Bo, Zhiqing Zhang, Ludo van Haasterecht, Peter Kloen, Thomas Rustemeyer, Laura Ventura, Lidiia Zhytnik, Elisabeth M. W. Eekhoff, Dimitra Micha and Marie Louise Groot
Int. J. Mol. Sci. 2025, 26(24), 11848; https://doi.org/10.3390/ijms262411848 - 8 Dec 2025
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Abstract
Osteogenesis imperfecta (OI) and Ehlers–Danlos syndrome (EDS) are inherited connective tissue disorders caused by diverse genetic defects, many of which affect collagen biosynthesis. However, the identified genetic variants do not always fully explain the clinical heterogeneity observed in patients, highlighting the need for [...] Read more.
Osteogenesis imperfecta (OI) and Ehlers–Danlos syndrome (EDS) are inherited connective tissue disorders caused by diverse genetic defects, many of which affect collagen biosynthesis. However, the identified genetic variants do not always fully explain the clinical heterogeneity observed in patients, highlighting the need for advanced models and imaging techniques to assess collagen structure and fibroblast behavior at the microscopic level. In this study, we employed 5-week three-dimensional (3D) dermal fibroblast cultures derived from patients with haploinsufficient (HI) and dominant-negative (DN) OI, EDS, and healthy controls. Using label-free higher harmonic generation microscopy (HHGM), we visualized and quantified secreted collagen fibers and fibroblast morphology in situ. We analyzed fibroblast 3D orientation, collagen fiber diameter, collagen amount per cell, and the spatial alignment between fibroblasts and collagen fibers. HI OI fibroblasts secreted significantly less collagen than both control and EDS-derived cells, while EDS samples exhibited thinner collagen fibers compared to controls. Across all groups, collagen fiber orientation was strongly correlated with fibroblast alignment, in line with the role of fibroblasts in matrix organization. In healthy controls and HI OI samples, we observed a depth-dependent, counterclockwise rotation in fibroblast orientation from the culture bottom to the surface—a pattern that was less prominent in DN OI and EDS samples, potentially reflecting altered matrix guidance in diseased tissues. Overall, the quantity and quality of collagen, as well as fibroblast morphology and organization, were markedly altered in the OI and EDS model systems. These alterations may mirror tissue-level manifestations of the diseases, demonstrating the physiological relevance of patient-derived 3D fibroblast models for OI and EDS, as well as the power of harmonic generation microscopy in probing the cellular and extracellular consequences of disease-related gene defects in collagen or its biosynthetic pathways. Extensions of this methodological approach provide a way towards deeper understanding of tissue-level manifestations of collagen dysregulation in connective tissue disorders. Full article
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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