Search Results (60,711)

Intrinsically disordered regions enable transcription factors (TFs) to undergo structural changes upon ligand binding, facilitating the transduction of environmental signals into gene expression. In this study, we applied molecular modeling methods to explore the hypothesis that unstructured inter-domain and subdomain linkers in bacterial TFs can function as sensors for carbohydrate signaling molecules. We combined molecular dynamics simulations and carbohydrate docking to analyze six repressors with GntR-type DNA-binding domains, including UxuR, GntR and FarR from Escherichia coli, as well as AraR, NagR and YydK from Bacillus subtilis. Protein models obtained from different time points of the dynamic simulations were subjected to sequential carbohydrate docking. We found that the inter-domain linker of the UxuR monomer binds D-fructuronate, D-galacturonate, D-glucose, and D-glucuronate with an affinity comparable to nonspecific interactions. However, these ligands formed multimolecular clusters, a feature absent in the UxuR dimer, suggesting that protein dimerization may depend on linker occupancy by cellular carbohydrates. D-glucose interacted with linkers connecting subdomains of the LacI/GalR-type E-domains in GntR and AraR, forming hydrogen bonds that connected distant structural modules of the proteins, while in NagR, FarR and YydK, it bridged the inter-domain linkers and a β-sheet within the HutC-type E-domains. Hence, our results establish flexible linkers as pivotal metabolic sensors that directly integrate nutritional cues to alter gene expression in bacteria. Full article

Gravity waves (GWs) are an important dynamic process in the planetary atmosphere. They are typically excited by convection, topography, or other sources from the lower atmosphere and propagate upwards. The GWs have a significant effect on the global atmospheric circulation on Mars. However, the lack of high-resolution data from previous observations has resulted in an insufficient understanding of GWs in the Martian atmosphere, particularly in terms of its global distribution and long-term evolution characteristics at different altitudes. Based on multiple years of Mars Climate Sounder (MCS) limb observations on board the Mars Reconnaissance Orbiter (MRO), we conducted a detailed study of the global distribution, seasonal and interannual variations in Martian atmospheric GWs with vertical wavelengths ranging from 9 to 15 km at three different altitude ranges, i.e., the low-altitude range of 200–20 Pa (Lp, ~10–30 km), the mid-altitude range of 20–2 Pa (Mp, ~30–50 km), and the high-altitude range of 2–0.2 Pa (Hp, ~50–70 km). The results indicate complex regional and north–south differences, as well as night–day variations, in the spatial distribution of GWs. Particularly, a three-wave structure of the GW activity is observed over mountainous regions in the mid-to-low latitudes of the Northern Hemisphere. The peak longitude range of this structure closely matches the mountainous terrain. In addition, our results reveal the presence of bands of GW aggregations in the mid- to-high latitudes of the Northern Hemisphere in the Mp and Hp layers, which may be caused by the instability of the polar jet. There are also obvious seasonal and interannual variations in GW activities, which are related to topography, polar jets, and large dust storms. The interannual variations in GWs imply that, in addition to the well-known large seasonal dust storms, complex interannual variations in atmospheric activity over the polar jets and in the complex topography at mid-to-low latitudes on Mars may also exist, which deserve further studies in the future. Full article

Freeze–thaw cycles (FTCs) are a prevalent weathering process that threatens the stability of canal slopes in seasonally frozen regions. This study combines direct shear tests under multiple F-T cycles with coupled thermo-hydro-mechanical numerical modeling to investigate the failure mechanisms of slopes with different moisture contents (18%, 22%, 26%). The test results quantify a marked strength degradation, where the cohesion decreases to approximately 50% of its initial value and the internal friction angle is weakened by about 10% after 10 freeze–thaw cycles. The simulation reveals that temperature gradient-driven moisture migration is the core process, leading to a dynamic stress–strain concentration zone that propagates from the upper slope to the toe. The safety factors of the three soil specimens with different moisture contents fell below the critical threshold of 1.3. They registered values of 1.02, 0.99, and 0.78 within 44, 44, and 46 days, which subsequently induced shallow failure. The failure mechanism elucidated in this study enhances the understanding of freeze–thaw-induced slope instability in seasonally frozen regions. Full article

This study aimed to determine the presence of relevant infectious and parasitic agents (IPAs) in managed honeybees from Central Italy and the Republic of Kosovo and Albania to assess the overall health status of local apiaries by determining the contamination levels and co-occurrence. Therefore, pathogens and parasites such as Paenibacillus larvae, Melissococcus plutonius, Vairimorpha apis, V. ceranae, the acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus variants DWV-A and DWV-B, and the parasitoid flies Megaselia scalaris and Senotainia tricuspis were detected by quantitative polymerase chain reaction (qPCR) and reverse transcriptase qPCR (RT-qPCR) in clinically healthy adult honeybees collected from 187 apiaries in the Abruzzo and Molise regions of Central Italy, 206 apiaries in the Republic of Kosovo in 2022 and 2023 and 18 apiaries in Albania in 2022. The percentages of positive samples and contamination for V. ceranae, P. larvae and DWV-B were significantly higher in the Republic of Kosovo and Albania, while the percentages of samples positive for M. plutonius, CBPV, DWV-A, and the parasitoid flies were higher in Central Italy. Additionally, P. larvae and some viruses showed significantly different occurrence rates between the two years in Italy and the Republic of Kosovo. The co-occurrence of IPAs also differed between the two geographic areas. Their varying distribution could depend on epidemiological dynamics, climatic factors, and management practices specific to each country, whose relative impact should be defined to guide targeted interventions to reduce honeybee mortality. Full article

Background: Drought stress is a primary environmental constraint limiting crop growth and productivity. Current drought-related plant research predominantly focuses on whole-leaf analyses, neglecting the spatial heterogeneity of metabolites within leaf tissues. Methods: This study combined transcriptomic and metabolomic approaches to investigate spatially distinct metabolic responses in marginal versus central regions of Medicago lupulina L. leaves under PEG-simulated drought. Results: Findings demonstrated that TCA cycle metabolites exhibited relative stability between leaf margins and centers under drought conditions, suggesting preserved core metabolic functionality in central tissues to sustain stress tolerance. Additionally, shikimic acid displayed a significantly reduced regional gradient in stressed tissues (PEG Margin vs. PEG Center) compared to controls. Phenylalanine, tryptophan, liquiritigenin, isoliquiritigenin, coproporphyrin III, and coproporphyrinogen III itself exhibited significantly increased internal gradient differences in stressed groups compared to control groups. The coordinated upregulation of key biosynthetic genes (e.g., TAT, AST, FNS II) in both the marginal and central regions of stressed leaves indicates a metabolic shift toward the biosynthesis of downstream defensive flavonoids. These metabolites and genes accumulated preferentially in margin regions of stressed leaves, indicative of localized activation of defense-associated metabolic pathways. Conclusions: This study reveals a spatially partitioned metabolic response to drought stress in M. lupulina leaves, where defensive metabolism is preferentially enhanced at the leaf margins while core metabolic homeostasis is maintained. These findings provide new spatial insights into plant drought acclimation and identify potential targets for improving crop resilience through the fine-tuning of local metabolism. Full article

Thrips are cosmopolitan agricultural pests and important vectors of plant viruses, and the increasing coexistence of multiple morphologically similar species has intensified the demand for species-specific molecular identification. However, traditional morphological identification and PCR assays using universal primers are often inadequate for mixed-species samples and field-adaptable application. In this study, we developed a species-specific molecular identification framework targeting a polymorphism-rich region of the mitochondrial cytochrome c oxidase subunit I (COI) gene, which is more time-efficient than sequencing-based COI DNA barcoding, for four economically important thrips species in southern China, including the globally invasive Frankliniella occidentalis. By aligning COI sequences, polymorphism-rich regions were identified and used to design four species-specific primer pairs, each containing a diagnostic 3′-terminal nucleotide. These primers were combined with a PBS-based DNA extraction workflow optimized for single-insect samples that minimizes dependence on column-based purification. The assay achieved a practical detection limit of 1 ng per reaction, demonstrated species-specific amplification, and maintained reproducible amplification at DNA inputs of ≥1 ng per reaction. Notably, PCR inhibition caused by crude extracts was effectively alleviated by fivefold dilution. Although the chemical identities of the inhibitors remain unknown, interspecific variation in inhibition strength was observed, with T. hawaiiensis exhibiting the strongest suppression, possibly due to differences in lysate composition. This integrated framework balances target specificity, operational simplicity, and dilution-mitigated inhibition, providing a field-adaptable tool for thrips species identification and invasive species monitoring. Moreover, it provides a species-specific molecular foundation for downstream integration with visual nucleic acid detection platforms, such as the CRISPR/Cas12a system, thereby facilitating the future development of portable molecular identification workflows for small agricultural pests. Full article

In the ecosystem, birds are important indicators that can sensitively reflect changes in the ecological environment and its health. However, bird monitoring has challenges due to species diversity, variable behaviors, and distinct morphological characteristics. Therefore, we propose a parallel dual-branch hybrid CNN–Transformer architecture for feature extraction that simultaneously captures local and global image features to address the “local feature similarity” issue in dual tasks of bird species and behaviors. The dual-task framework comprises three main components: the Token Re-segmentation Module (TRM), the Multi-scale Adaptive Module (MAM), and the Feature Interleaving Structure (FIS). The designed MAM fuses hybrid attention to address the problem of different-scale birds. MAM models the interdependencies between spatial and channel dimensions of features from different scales. It enables the model to adaptively choose scale-specific feature representations, accommodating inputs of different scales. In addition, we designed an efficient feature-sharing mechanism, called FIS, between parallel CNN branches. FIS interleaving delivers and fuses CNN feature maps across parallel layers, combining them with the features of the corresponding Transformer layer to share local and global information at different depths and promote deep feature fusion across parallel networks. Finally, we designed the TRM to address the challenge of visually similar but distinct bird species and of similar poses with distinct behaviors. TRM adopts a two-step approach: first, it locates discriminative regions, and then performs fine segmentation on them. This module enables the network to allocate relatively more attention to key areas while merging non-essential information and reducing interference from irrelevant details. Experiments on the self-made dataset demonstrate that, compared with state-of-the-art classification networks, the proposed network achieves the best performance, achieving 79.70% accuracy in bird species recognition, 76.21% in behavior recognition, and the best performance in dual-task recognition. Full article

At present, the global shortage of water resources has led to serious challenges, and traditional water production technologies such as seawater desalination and atmospheric water harvesting have certain limitations due to inflexible operation and environmental conditions. This study proposes a novel water production system (called “NeWater” system in this paper), which combines saline water desalination with atmospheric water-harvesting technologies to simultaneously produce freshwater from brackish water or seawater and ambient air. To evaluate its performance, an integrated thermodynamic and mathematical model of the system was developed and validated. The NeWater system consists of a vapor compression refrigeration unit (VRU), a direct evaporation unit (DEU), up to four heat exchangers, some valves, and auxiliary components. The system can be applied to areas and scenarios where traditional desalination technologies, like reverse osmosis and thermal-based desalination, are not feasible. By switching between different operating modes, the system can adapt to varying environmental humidity and temperature conditions to maximize its freshwater productivity. Based on the principles of mass and energy conservation, a performance simulation model of the NeWater system was developed, with which the impacts of some key design and operation parameters on system performance were studied in this paper. The results show that the performances of the VRU and DEU had a significant influence on system performance in terms of freshwater production and specific energy consumption. Under optimal conditions, the total freshwater yield could be increased by up to 1.9 times, while the specific energy consumption was reduced by up to 48%. The proposed system provides a sustainable and scalable water production solution for water-scarce regions. Optimization of the NeWater system and the selection of VRUs are beyond the scope of this paper and will be the focus of future research. Full article

As global production networks become increasingly regionalized, diversified, and resilience-oriented, metropolitan areas (MAs) have emerged as important spatial platforms for industrial development. This study examines whether China’s national-level metropolitan area integration policies promote urban industrial structure upgrading and, if so, through which channels. We first develop a set of conceptual mechanisms and hypotheses, and then test them using panel data for 281 prefecture-level cities in China from 2012 to 2022. A staggered difference-in-differences (DID) model, complemented by a series of robustness checks, is employed to identify the policy effects. The baseline estimates indicate that the industrial structure of MA member cities is, on average, about 2.43 percentage points more advanced than that of non-MA cities. Mechanism analysis shows that the policies foster urban industrial upgrading through unified market formation, technological improvement, and optimization of factor endowments. However, the policies have only a very limited impact on breakthroughs in cutting-edge or frontier technologies. Based on these findings, we propose targeted policy recommendations to address the identified shortcomings. Full article

Background: Head and neck (H&N) cutaneous melanomas have poorer outcomes than melanomas at other sites, yet sentinel lymph node biopsy (SLNB)—a key prognostic tool in clinically node-negative disease—is less frequently performed, particularly outside tertiary centers. We evaluated the feasibility and prognostic relevance of SLNB in a medium-volume regional institution. Methods: We retrospectively reviewed patients with primary H&N cutaneous melanoma who underwent SLNB at the Department of Oral and Maxillofacial Surgery, University of Szeged, between 2010 and 2022. Clinicopathological features, nodal outcomes, recurrence patterns, recurrence-free survival (RFS), and overall survival (OS) were analyzed using Kaplan–Meier methods and univariate Cox regression. Results: Thirty-eight patients underwent SLNB, with a 100% sentinel lymph node identification rate and no major complications. Positive sentinel lymph nodes were identified in 8 patients (21.1%). Two false-negative events occurred, resulting in a false-omission rate of 6.7% and a negative predictive value of 93.3%. SLN-negative patients demonstrated longer RFS and OS, although differences were not statistically significant. Among patients with intermediate-risk melanoma (pT1b–pT3a), 18.5% had a positive SLN. Conclusions: SLNB is a safe and clinically meaningful staging procedure for H&N melanoma in a medium-volume regional center. Sentinel node status provides important prognostic information and supports appropriate patient selection for contemporary adjuvant therapy. Full article

Accurate lightning data are critical for disaster warning and climate research. This study systematically compares the Fengyun-4A Lightning Mapping Imager (FY-4A LMI) satellite and the Advanced Time-of-arrival and Direction (ADTD) lightning location network in the Beijing-Tianjin-Hebei (BTH) region (April–August, 2020–2023) using coefficient of variation (CV) analysis, Welch’s independent samples t-test, Pearson correlation analysis, and inverse distance weighting (IDW) interpolation. Key results: (1) A significant systematic discrepancy exists between the two datasets, with an annual mean ratio of 0.0636 (t = −5.1758, p < 0.01); FY-4A LMI shows higher observational stability (CV = 5.46%), while ADTD excels in capturing intense lightning events (CV = 28.01%). (2) Both datasets exhibit a consistent unimodal monthly pattern peaking in July (moderately strong positive correlation, r = 0.7354, p < 0.01) but differ distinctly in diurnal distribution. (3) High-density lightning areas of both datasets concentrate south of the Yanshan Mountains and east of the Taihang Mountains, shaped by topography and water vapor transport. This study reveals the three-factor (climatic background, topographic forcing, technical characteristics) coupled regulatory mechanism of data discrepancies and highlights the complementarity of the two datasets, providing a solid scientific basis for satellite-ground data fusion and regional lightning disaster defense. Full article

Alkane is a predominant wax component, whose production requires the aids of CER1 and CER3. In rice, OsCER1 and OsCER3 are present in multiple copies. Until now, the roles of these genes have been studied individually; however, a systematic comparison of their relative contributions to cuticular wax biosynthesis has not yet been carried out. Phylogenetic tree analysis revealed that CER1s and CER3s from different plants are classified into two subgroups. RT-qPCR analysis showed that these genes display distinct expression patterns, revealing their specific roles in wax production. Promoter prediction analysis showed that cis-elements responding to light, phytohormones and stress are enriched in the promoter region of OsCER1s and OsCER3s. These proteins are all localized in the endoplasmic reticulum. Further study showed that OsCER1s and OsCER3s are inclined to form a complex during the wax synthesis. Finally, the wax analysis of single mutants showed that among the examined genes, OsCER3a mutation greatly reduced the total wax amounts to 19.6% of wild-type plant with a decrease in most of wax components, whereas mutation of other genes including OsCER3b, OsCER3c, OsCER1a and OsCER1c slightly or barely affect wax production, suggesting that OsCER3a plays major roles in rice wax production whereas other proteins redundantly participate in the wax synthesis. Additionally, the wax increasing rates of Arabidopsis expressing OSCER1 are lower than those of overexpressing AtCER1. Taken together, our study identified the predominant genes involved in wax production, which will be useful for genetically engineering rice with enhanced stress tolerance. Full article

Drastic cropland expansion and its internal structural changes have had an obvious impact on agricultural landscapes and ecosystem services. However, a prolonged investigation of this effect is still lacking in China’s grain-producing bases, such as Sanjiang Plain. To address this issue, half a century of study on the ‘land trajectory migration–landscape evolution–ecological effect,’ covering the period 1970–2020, was elucidated using the synergistic methodology of spatial analysis technology, the reclamation rate algorithm, the landscape indicator, and the newly established ecosystem service improvement model. Satellite observation results indicate that the cropland area exhibited a substantial expansion trend from 23,672.69 km² to 42,856.17 km² from 1970 to 2020, representing a net change of +19,183.48 km² and a huge growth rate of 81.04%, which led to an obvious improvement in the level of agricultural cultivation. Concurrently, the internal structure of the cropland underwent dramatic restructuring, with rice fields increasing from 6.46% to 53.54%, while upland fields decreased from 93.54% to 46.46%. In different regions, spatially heterogeneous improvements of 2.64–52.47% in agricultural cultivation levels across all cities were observed. From 1970 to 2020, the tracked cropland center of gravity trajectories exhibited a distinct biphasic pattern, initially shifting westward and then followed by a southward transition, accumulating a displacement of 19.39 km². As for the evolved agricultural landscapes, their integrity has improved (SHDI = −0.08%), accompanied by increased connectivity (CON = +8.82%) and patch edge integrity (LSI = −15.71%) but also by reduced fragmentation (PD = −48.14%). Another important discovery was that the evaluated ecosystem services continuously decreased from 2337.84 × 10⁸ CNY in 1970 to 1654.01 × 10⁸ CNY in 2020, a net loss of −683.84 × 10⁸ CNY and a huge loss rate of 33.65%, accompanied by a center–periphery gradient pattern whereby degradation propagated from the low-value central croplands to the high-value surrounding natural covers. These discoveries will play a significant role in guiding farmland structure reformation, landscape optimization, and ecosystem service improvement. Full article

Background/Objectives: Diffusion-weighted imaging (DWI) is a magnetic resonance technique used to map the apparent diffusion coefficient (ADC) of water in human tissue. ADC assessment plays a central role in clinical diagnostics, as malignant tissues typically exhibit reduced water mobility and, thus, lower ADC values. Accurately measuring the ADC requires effective fat suppression to prevent contamination from the residual fat signal, which is commonly believed to cause ADC underestimation. This study aimed to demonstrate that ADC overestimation may occur as well. Methods: Our theoretical analysis shows that out-of-phase conditions between fat and water signals lead to ADC overestimations. We performed demonstration experiments on fat–water phantoms and the breasts of 10 healthy female volunteers. In particular, we considered three out-of-phase conditions: First and second, short-time inversion recovery (STIR) fat suppression with incorrect inversion time and incorrect flip angle, respectively. Third, phase differences due to spectral fat saturation. The ADC values were assessed in regions of interest (ROIs) that included both water and residual fat signals. Results: In the phantoms and the volunteer data, ROIs containing both fat and water signals consistently exhibited lower ADC values under in-phase conditions and higher ADC values under out-of-phase conditions. Conclusions: We demonstrated that out-of-phase conditions can result in ADC overestimation in the presence of residual fat signals, potentially resulting in false-negative classifications where malignant lesions are misinterpreted as benign due to an elevated ADC. Out-of-phase fat and water signals might also reduce lesion conspicuity in high b-value images, potentially masking clinically relevant findings. Full article

A new desmid microalga species, Cosmarium yassinii A.A. Saber, El-Sheekh, Kouwets et Cantonati sp. nov., was isolated from two hyper-arid mountain valleys, so-called “wadis”, in the Eastern Desert of Egypt. The distinctive morphological features of this new species were established using light and scanning electron microscopy observations, and also by documenting its life-cycle stages. Taxonomically, C. yassinii is characterized by a cell wall sculpture consisting of isolated granules or small warts arranged circularly in the swollen mid-region of each semicell, never forming parallel vertical ridges or costae as in morphologically similar species, and the interesting shape of the marginal granules appears as small emarginate “combs” or crenae, including its knobby zygospores. Similarities and differences with the morphologically most closely related species are discussed in detail. Ecologically, C. yassinii seems to prefer alkaline freshwater environments with lower nutrient concentrations and a NaCl/HCO₃ water type. The detailed assessment and documentation of the biodiversity of these peculiar freshwater ecosystems are a fundamental prerequisite to adequately inform their protection strategies. Full article