Search Results (296)

Background/Objectives: Docosahexaenoic acid (DHA) is an essential component of breast milk for infant neurocognitive and visual development. Because DHA depends largely on dietary intake, women with low seafood consumption are at increased risk of inadequate DHA contents in breast milk. This study aims to quantify the DHA content in breast milk and assess how recommended dietary supplementation during lactation affects these contents among lactating women in three Balkan countries. Methods: This cross-sectional multicenter study included 128 lactating women (18–35 years). Dietary intake of DHA was assessed using a modified Food Frequency Questionnaire. DHA in breast milk was measured by gas chromatography following standard protocols. Both the FFQ and breast milk samples were collected at two time points: 20–40 (visit 1—V1) and 80–100 days (visit2—V2) postpartum. Results: In lactating women, seafood intake was low (Visit 1: 37.5 (0.0–93.8) g/week), meeting about 28% of the recommended amount, with some variation between countries. Consequently, the mean dietary n-3 PUFA and DHA intake for the total sample was less than 50% and 5% of the recommended daily allowance, respectively. Correlation analysis indicates that DHA content in breast milk is related to dietary DHA intake (Visit 1: r = 0.258, p = 0.001; Visit 2: r = 0.238, p = 0.012). DHA contents failed to reach the 0.3% clinical threshold for optimal neurodevelopment, with average levels ranging from 0.149 to 0.165%. Furthermore, no significant differences were observed between supplemented and unsupplemented women at either time point, highlighting a persistent regional deficit regardless of supplementation status. Conclusions: Lactating women in the Balkan region have a low dietary intake of n-3 PUFA and DHA, which is reflected in the breast milk lipid profile, regardless of standard supplementation. These findings highlight the need for a targeted nutritional strategy both via nutrition and supplementation to improve DHA intake in lactating women, but more importantly in infants. Full article

This study evaluated trace element bioaccumulation in Nile tilapia (Oreochromis niloticus) marketed in Brazil and assessed potential implications for food safety and human health. A total of 120 farmed fish samples, including whole fish and tissues (gills, kidneys, liver, and muscle), were analyzed. Concentrations of Al, As, Cr, Cu, Fe, Mn, Ni, Pb, Se, and Zn were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) and compared with ANVISA and FAO/WHO guideline values. Arsenic concentrations complied with ANVISA limits, whereas Pb in whole Nile tilapia exceeded the permissible threshold. Nickel and Se concentrations in muscle and whole fish also surpassed FAO/WHO guidelines. Estimated Se intake from consumption of whole tilapia and muscle (fillet) reached 188.01% of the Recommended Dietary Allowance (RDA) for children aged 8 years. Contamination index values (CI) indicated bioaccumulation of Ni in multiple tissues and Se in edible portions. Human health risk assessment identified carcinogenic risks associated with As, Cr, Ni, and Pb, while non-carcinogenic risk (HI > 1) was primarily driven by As exposure. These findings highlight the need for continuous monitoring of trace elements in aquaculture products. Full article

Background/Objectives: Sapropterin dihydrochloride is an established treatment option for individuals with phenylketonuria (PKU) who demonstrate responsiveness, but uncertainty persists regarding dosing frequency, timing relative to meals, the influence of dietary composition, and efficacy of different formulations. Despite widespread use in the UK, real-world administration behaviours have not previously been characterised. This study aimed to characterise sapropterin administration behaviours among people with PKU in the UK. Methods: A 31-item questionnaire was developed and disseminated via the National Society for Phenylketonuria website and social media channels. The survey captured demographic information, dosing schedules, formulation use, administration techniques, co-ingestion with food, and changes in natural protein tolerance following initiation of generic sapropterin. Results: 124 current sapropterin users completed the survey. Most respondents were caregivers of children or adolescents (68.5% aged 0–18 years). Once-daily dosing was most common (66.1%, n = 82), typically administered at breakfast, followed by twice-daily (32.3%, n = 40) and three-times-daily (1.6%, n = 2). Tablets were the predominant formulation (92.7%, n = 115); 50.4% (n = 58/115) swallowed tablets whole, while the remaining (49.6%, n = 57/115) crushed or dissolved them in water or juice. Nine respondents (7.3%, n = 9/124) used powder sachets. Most participants (75%, n = 93/124) took sapropterin with food, with both low-fat (36.6%, n = 34/93) and high-fat (26.9%, n = 24/93) meals reported. Over a third of participants (33.9%, n = 42/124) tolerated a natural protein intake >30 g/day when this was measured, and a further 15.3% (n = 19) were able to maintain a fully unrestricted protein intake without protein substitute supplementation. The magnitude of protein intake improvement was significantly greater among adults (p < 0.001), those with higher baseline natural protein intake (≥30 exchanges/day) (p < 0.001), and individuals who swallowed sapropterin tablets whole (p = 0.038). Although 71.8% (n = 89/124) were pleased with their increased natural protein allowance, many expressed a desire for further improvement. Conclusions: Substantial heterogeneity in dosing schedules, formulation handling, and co-ingestion practices highlights the absence of standardised guidance. These findings emphasise the need for clearer clinical recommendations to optimise treatment effectiveness and support consistent, equitable care. Full article

Selenium (Se) is essential for human health, but its dietary intake remains insufficient in many regions, increasing interest in biofortification strategies. Indoor hydroponic systems offer a controlled and resource-efficient approach for producing Se-enriched leafy greens. Sulfur (S), an essential macronutrient for plants, affects Se uptake and metabolism due to their chemical similarity. In this study, we investigated the effects of Se supplementation (2 µM Na₂SeO₄) under two S levels (0.65 and 1.3 mM, supplied as MgSO₄) on Se accumulation, yield, and nutritional quality in lettuce, rocket, and basil grown in an indoor nutrient film technique (NFT) system. High S supply increased biomass in lettuce and basil by 16% and 25%, respectively, while rocket remained unaffected. The effect of Se on biomass depended on S status and species. Under low S conditions, Se increased lettuce biomass but reduced basil biomass, whereas no significant effects were observed under high S. Sulfur strongly reduced Se accumulation in all species, leading to lower contributions to the recommended daily allowance (RDA). Under low S conditions, Se-biofortified lettuce, rocket, and basil provided 111%, 179%, and 37% of the RDA per serving, respectively, whereas these values decreased to 56%, 64%, and 20% under high S. Sulfur and Se treatments also influenced macro- and micro-nutrient composition in a species-dependent manner. Se supplementation consistently reduced total phenolic content and antioxidant capacity (DPPH and FRAP) across all species. Total ascorbic acid was affected only in rocket, with the highest levels observed under high S without Se. These findings highlight a clear antagonistic interaction between S and Se in hydroponic systems and demonstrate the need to optimize S supply to balance yield and Se biofortification without compromising nutritional quality in leafy greens grown in indoor systems. Full article

Guanidinoacetic acid (GAA) and methionine are two biologically active compounds related to cell energy metabolism. Both are metabolically linked, as the methyl group needed to transform GAA into creatine derives from S-adenonsylmethionine out of the homocysteine cycle. A broiler feeding study was conducted to evaluate potential interactions between 600 g/t supplemental GAA and feed additives with methionine activity. The experimental design comprised two parts. Part one consisted of three dietary treatments in which DL-methionine (DL-Met) was added to meet sulfur amino acid recommendations (1), DL-Met was replaced with liquid methionine hydroxy analogue-free acid (MHA-FA) assuming an 80% methionine activity in the product (2), and DL-Met was added at 65% of the MHA-FA dose used in Treatment 2 (on a product weight basis). Treatments 1, 2, and 3 reflect the range of commercial practice with assuming bioefficacies of either 80% (Trt 2 vs. 1) or 65% (Trt 3 vs. 2) for MHA-FA relative to DL-Met. Part two followed a 2 × 4 factorial design. Either DL-Met or MHA-FA (assuming 80% methionine activity) was added to diets with gradually reduced apparent metabolizable energy (AME; standard, −25, −50, −75 kcal/kg AME). For MHA-FA, a bioefficacy of 80% relative to DL-Met was applied in order to evaluate interactions with GAA efficiency assuming inadequate dietary Met+Cys supply with MHA-FA. AME-reduced diets were supplemented with 600 g/t GAA to evaluate its energy-sparing potential. A total of 2784 male Cobb 500 broilers were equally assigned to 116 floor pens, resulting in 13 replicates (one treatment with 12) per treatment. A five-phase feeding program was introduced from 1 to 40 days of age. Body weights (BW) and feed intake (FI) were recorded in weekly intervals allowing for calculating weight gain (BWG) and feed conversion ratio (FCR). At termination of the experiment, three birds per pen were obtained for carcass analysis. The results revealed that an overestimation of the bioefficacy of MHA-FA relative to DL-Met significantly compromised broiler growth (p < 0.05), while applying a bioefficacy of 65% for MHA-FA relative to DL-Met65 resulted in similar performance. However, on day 40, BW of broilers fed DL-Met65 were higher than with MHA-FA (p < 0.05). It is concluded that an overestimation of the bioefficacy of MHA-FA relative to DL-Met bears the risk of compromised broiler performance while a bioefficacy of 65% allows at least similar, potentially even better, performance and economic advantages. There were basically no interactions between MHA-FA or DL-Met and graded ME levels with concomitant GAA supplementation regarding overall performance of broilers. This means that final BW of DL-Met-fed broilers were higher than those of MHA-FA broilers independent of dietary ME (p < 0.05). In early feeding phases, interactions indicated that broiler performance (BW day 7, 21, p < 0.05) diminished with dietary energy reduction with DL-Met supplementation while this was not the case with MHA-FA-fed broilers. However, with standard AME, DL-Met outperformed MHA-FA (p < 0.05). Basically, GAA supplementation compensated AME reduction up to 50 kcal/kg as there were no differences in BW, BWG, FCR, FI, or breast meat yield. Additionally, −75 kcal/kg AME was compensated by GAA only up to a broiler age of 21 days. Full article

Background: Dietary management of phenylketonuria (PKU) focuses on restricting phenylalanine (Phe) intake. The European PKU guidelines and the PKU Dietary Handbook recommend a simplified PKU diet, allowing unrestricted consumption of many low-Phe foods, called free foods. While this approach may reduce the treatment burden for patients, its implementation varies. This study investigated practices of Swedish metabolic dietitians regarding the simplified diet and the use of free foods for classical PKU (cPKU) and compared these with European recommendations. Methods: A survey was distributed to all metabolic dietitians in Sweden. The survey included questions on professional experience, the use of free foods and the classification of 135 low-protein food items as never, sometimes or always counted in cPKU. Data were summarised descriptively. Results: All 13 eligible dietitians participated. The use of free foods was recommended by 8/13 dietitians. Of foods classified as free in the PKU Handbook, about one third were commonly restricted in Swedish PKU practice. For 39% of the foods surveyed, no single response option (never, sometimes or always counted) reached >50%, indicating variation in practice. Classification of individual foods partially aligned with their Phe content, but portion size and concerns about excessive Phe intake also influenced advice. Conclusions: Significant variation exists in the dietary management of PKU in Sweden, and the simplified diet approach is not consistently implemented. Dietitians’ concerns about the safety of increased Phe intake from free foods play a central role in this. These findings highlight challenges in incorporating international guidelines into national practice and underscore the need for further research to address dietitians’ safety concerns related to the simplified diet. Full article

Background/Objective: Rapid, validated dietary screening tools are lacking for individuals with spinal cord injury (SCI), where routine clinical check-ups do not allow sufficient time for extensive dietary assessments typically required to evaluate adherence to dietary recommendations. We developed a 15-item dietary screener (SCI NutriTool) and evaluated its accuracy in classifying non-adherence to a healthy food pyramid compared with a validated food frequency questionnaire (FFQ). Methods: The SCI NutriTool was developed through literature review and expert consensus. In a validation study, 51 adults with SCI (mean age 57.0 years; 76.5% men; 68.8% traumatic injury) completed the SCI NutriTool twice and a validated 97-item FFQ, which served as the reference method. Results: The SCI NutriTool demonstrated substantial variability in performance across food groups, reflecting its domain-specific screening properties. Sensitivity was high for fruits and vegetables (91.7%), protein-rich foods (90.5%), and sweetened/alcoholic beverages and snacks (82.4%), with relatively high positive predictive values (PPV: 73.7–90.5%), supporting the tool’s ability to identify individuals who are likely non-adherent and may benefit from further nutritional assessment or counselling. In contrast, for starchy foods and nuts, oils, and fatty spreads/sauces, sensitivity was low (20.0% and 50.0%), while specificity was modest. This indicates that the tool performs better in correctly identifying adherent individuals in these domains, which is reflected in higher negative predictive values (NPV: up to 94.1%). However, the low sensitivity suggests that individuals with non-adherence may be missed, limiting the tool’s usefulness as an early screening trigger for these food groups. Conclusions: The SCI NutriTool’s performance varies across food groups, demonstrating a stronger ability to identify non-adherence in protein-rich foods, fruit and vegetables, sweetened and alcoholic beverages, and snacks, but limited discriminatory capacity for others. In particular, it is not suitable for screening non-adherence to starchy foods and fats. Accordingly, it is best used as a triage tool to guide further dietary assessment and targeted nutritional interventions rather than as a standalone diagnostic instrument. Full article

Background/Objectives: Micronutrient malnutrition, particularly deficiencies in calcium, vitamin D, iron, zinc, and iodine, remains a significant public health issue among school-aged children in Morocco. Processed cheese, such as “The Laughing Cow” (TLC), has potential as a vehicle for fortification due to its widespread consumption and accessibility. This study aimed to evaluate the impact of fortified TLC on micronutrient intake and adequacy relative to the Recommended Dietary Allowances (RDA), among Moroccan children aged 6–12 years, and to explore differences in effects by socioeconomic status (SES). Methods: Data from the Moroccan Household Budget Survey (2013–2014) included 9266 children (39.4% TLC consumers). Dietary intake was assessed using 24 h recalls, and nutrient composition was analyzed using Ciqual 2020 tables and specialized software. Fortification scenarios were modelled to estimate potential impacts on micronutrient intake and compliance with RDAs. Results: Under the modelling scenarios, consumption of one portion/day of fortified TLC significantly improved RDAs compliance for iron, iodine, and zinc (p < 0.05). There was also an increase in RDA compliance for calcium and vitamin D, but differences were not significant. The impact of fortification on micronutrient intake and RDA compliance increased with socioeconomic status. Consumers of more than one portion/day showed the highest compliance with RDAs (p < 0.001). Fortification effects were consistent across age subgroups. Conclusions: Fortifying processed cheese represents a feasible strategy to address micronutrient deficiencies among Moroccan schoolchildren. This study highlights the potential of targeted fortification programmes to improve public health outcomes, particularly in vulnerable populations. Further research is needed to optimize fortification approaches and ensure sustainability. Full article

Mycotoxin contamination is an important threat to food and feed safety as well as human and animal health, with particular emphasis on oxidative stress, apoptosis, autophagy, inflammation, and dysbiosis. Mycotoxins represent major health threats because they disturb cellular homeostasis and induce oxidative damage. Nutritional factors, such as dietary antioxidants and bioactive chemicals, can influence the body’s reaction to mycotoxin exposure, either reducing or increasing its effects. This study discusses how mycotoxins (aflatoxin B1, deoxynivalenol, and ochratoxin A) induce oxidative stress by producing reactive oxygen species (ROS)-mediated DNA damage, which induces cellular damage and activates apoptosis, an intended cell death process that is critical for tissue integrity. Furthermore, mycotoxins alter autophagy, a cellular degradation process that can be beneficial or destructive depending on the situation, affecting cell survival. The inflammatory response is particularly important because mycotoxin-induced oxidative stress and cell damage activate inflammatory pathways, which contribute to tissue injury and disease progression. Nutritional factors high in antioxidants, anti-inflammatory substances (Lycopene, Curcumin, Thyme oil, Gum Arabic, and Ginger), probiotics, and prebiotics show potential in mitigating these negative consequences by reducing oxidative stress and inflammation. Advances in molecular biology and omics technologies (transcriptomics, proteomics, metabolomics, and single-cell sequencing) can lead to better knowledge of the underlying pathways, allowing for more tailored nutritional recommendations and medicinal interventions. Finally, combining dietary modulation with mycotoxin risk management is a viable path for protecting health and increasing resilience to mycotoxin-related toxicities in animals. Full article

Pancreatic ductal adenocarcinoma (PDAC), which remains undetected till advanced stages of progression, is becoming a more common and aggressive PC with high mortality rates. Trending unhealthy dietary habits, sedentary life, obesity, diabetes, high body mass index (BMI), and perturbed gut microbiota, also known as dysbiosis, increase the PDAC incidences. There is an urgent need to delve into reliable and cost-effective biomarkers and strategies to diagnose and prevent the disease. Microbial remedies and dietary phytonutrients that restore gut microbial balance may be the prospective preventive measures. This article intends to recommend the hypothesis that PDAC is incepted and characterized by microbial signatures and can be managed by integrating evidenced-based complimentary approaches entailing diet, nutraceuticals, microbial interventions and life style modifications with standard therapies. The futuristic longitudinal and mechanistic studies to unravel host–microbiota and the host–microbiome interactions will allow deeper aetiologic and mechanistic insights into PDAC to evolve therapies and recommendations for better pancreatic health. Full article

Background: There is a growing number of studies suggesting the effectiveness of dietary supplements in preventing and treating endometriosis. It has been suggested that deficiencies in vitamins D and E as well as zinc are associated with the increased risk of endometriosis development. Beneficial effects of magnesium, curcumin, resveratrol and epigallocatechin-3-gallate were found in experimental animal studies. A reduction in pain related to endometriosis was shown in women using omega-3 and alpha-lipoic acid. Meanwhile, decreasing endometriotic lesion size after the supplementation of omega-3, N-acetylcysteine, vitamin C and epigallocatechin-3-gallate was observed in animal and human studies. Thus, the aim of this critical review was to summarize the available data describing the effects of dietary supplements used in the treatment of endometriosis. Material and Methods: The PubMed, Embase, Cochrane, and Web of Science databases were searched for related studies until 15 December 2025. Finally, 34 studies were included in the synthesis. Results: Of these 34 studies, only 23 were randomized, placebo-controlled trials. There have been no RCTs evaluating the effectiveness of vitamin E, zinc, alpha-LA, EGCG and DIM in the treatment of endometriosis. Single studies evaluating the effectiveness of vitamin C, magnesium, resveratrol, NAC and PEA with PLD have not confirmed it. Meanwhile single studies evaluating the effectiveness of selenium, propolis and quercetin have confirmed it. Of the four studies assessing the effectiveness of vitamin D, two confirmed it and two did not; of the two studies assessing probiotics, one confirmed its effectiveness and one did not; of the two studies assessing curcumin, one confirmed its effectiveness and one did not; and of the three studies assessing omega-3, two confirmed its effectiveness and one did not. All four RCTs assessing the combination of vitamins C and E confirmed their effectiveness. Conclusions: Despite encouraging observations from experimental studies, the results of RCTs are less encouraging and do not allow for the formulation of recommendations concerning the use of supplements in the treatment of endometriosis symptoms according to EBM. Full article

Background/Objectives: Excessive sodium (primarily from sodium chloride, NaCl) intake remains one of the leading dietary risk factors associated with hypertension, cardiovascular disease, and other chronic health conditions worldwide. Commercial diet catering services providing ready-to-eat daily meal plans have become increasingly popular and are often perceived as nutritionally balanced; however, analytical evidence regarding their actual salt content remains limited. The aim of this study was to evaluate the NaCl content of daily food rations (DFRs) offered by commercial diet catering services in Poland. Methods: A total of 120 DFRs representing three dietary patterns (Hashimoto diet, DASH diet, and low-carbohydrate diet) were collected from 40 catering providers. Sodium chloride content was determined using the Mohr titration method. Sodium intake values were estimated by conversion from NaCl equivalents to allow comparison with dietary recommendations. Results: The median NaCl content across all analyzed diets was 14.19 g/day (Q1: 10.62 g; Q3: 17.49 g), corresponding to approximately 284% of the World Health Organization recommended maximum intake of 5 g/day of salt. Nearly half of the analyzed DFRs (45.83%) exceeded the recommended intake by more than threefold. Overall, 99.2% of the analyzed DFRs exceeded recommended NaCl intake levels, while 91.9% did not comply with the values declared by manufacturers. DFRs consisting of five meals contained higher NaCl levels than three-meal plans (p < 0.0196); however, this difference may be related to variation in total food mass rather than meal frequency, as the number of meals was confounded with diet type. Conclusions: These findings suggest that commercially prepared diet catering meals may represent a substantial source of dietary NaCl when used as a primary daily food source. Improved nutritional monitoring, clearer nutrient reporting, and quality control of commercially prepared dietary plans may support public health strategies aimed at reducing NaCl intake. Full article

Cowpeas are a legume widely consumed in Brazil. Given this, the objective of this study was to investigate the presence of metals (loids) in pods and leaves of Vigna unguiculata located near a highway with high vehicle traffic and a landfill, and to assess possible risks to human health. Pod and leaf samples were collected at nine points between the highway and the landfill. The elements were analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES) and quantified. The risk to human health was assessed using risk quotient and risk index values. A quantitative analysis of the chemical elements was also performed using the maximum tolerable intake level. Element concentrations were higher in the leaves than in the pods. The human health risk analysis showed that the average daily consumption of both pods (44 g/day) and leaves (67 g/day) may pose a chronic health risk to adult men and women, due to simultaneous exposure to multiple metals. It was concluded that the plant is contaminated and that its ingestion can cause toxicity, warranting warnings against cultivating areas near anthropogenic activities that may be contaminated with heavy metals, thereby affecting nutritional safety. Full article

A reassessment of the Trp requirements for brown laying hens using the Trp biomass source allows an update of the nutritional recommendation, in addition to assessing whether biomass has an adjuvant effect in the diet. This research was carried out with the aim of evaluating the efficiency utilization of Trp and determining the ideal Trp intake for aged brown laying hens using the L-Trp biomass 60%. A completely random design was used, with eight treatments and eleven replicates, totaling 264 hens. The diets were formulated by supplementation technique using one basal diet with 0.112% of digestible Trp, resulting in amino acid levels that ranged from 0.112 to 0.225% of digestible Trp. The variables analyzed were performance and egg quality. When the effect of Trp levels (p ≤ 0.05) was detected, the model’s linear–plateau (LP), quadratic–plateau (QP), and the first intercept of the QP in the plateau of LP were adjusted to determine the ideal Trp intake. The results obtained show an effect of the treatments (p < 0.05) only on the performance variables. The first intercept results obtained were 186.2, 174.7, 182.7, and 182.5 mg/hen per day for egg production, egg mass, feed conversion ratio, and feed efficiency, respectively. Dietary supplementation with L-Trp biomass 60% effectively meets the nutritional requirements of aged brown laying hens, supporting efficient nutrient utilization without impairing productive performance or egg quality. Full article

The prevalence of diet-related noncommunicable diseases (NCDs; e.g., obesity, type 2 diabetes, cardiovascular disease, and certain cancers) is increasing globally, while food systems are also driving climate change and biodiversity loss. Transitioning to predominantly plant-based (“plant-forward”) dietary patterns can improve health and lower environmental impacts. We present the Slovenian Nutrition Guidelines 2025 (SNG2025)—their methodology, development, and core recommendations. Developed as adult food-based dietary guidelines, the SNG2025 are evidence-informed, drawing on the scientific literature, national nutritional data, and expert consensus. We set quantitative daily intake targets by integrating evidence on primary NCD outcomes with environmental metrics (greenhouse gas emissions, land use, and water use), which led to upper limits for animal-based foods. The recommended plant-forward dietary pattern, aligned with the EAT–Lancet planetary health diet, emphasises vegetables, fruits, legumes, whole grains, nuts, seeds, and unsaturated oils; allows low-to-moderate amounts of seafood, poultry, dairy, and eggs; and keeps red and processed meat, free sugars, refined grains, saturated fat, salt, ultra-processed foods (UPFs), and alcohol to a minimum. For the first time, we operationalise health, environmental sustainability, and equity (cultural diversity and accessibility) through a three-tier, plant-forward food plate model (Mediterranean, vegetarian [lacto-ovo], and whole food, plant-based [vegan]). The SNG2025 aim to reduce the risk of NCDs, lower the dietary environmental footprint, and improve fair access to healthy food. They signal a shift from disease management to a prevention-oriented, systems approach that aligns health and ecological goals. With robust implementation, supportive policies, and multisector collaboration, the SNG2025 can strengthen population health, foster more resilient food systems, and advance equity and long-term sustainability. Full article