Search Results (1,465)

Background/Objectives: Recent evidence challenges the classical chemiosmotic theory, suggesting that proton movement along membrane surfaces—not bulk-phase gradients—drives bioenergetic processes. Proton accumulation on membranes like the myelin sheath and endoplasmic reticulum (ER) may represent a universal mechanism for cellular energy storage. This study investigates whether phospholipids from these membranes, combined with anionic bee venom proteins, enhance proton absorption, potentially elucidating a novel bioenergetic pathway. Methods: Five phospholipids (phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, sphingomyelin, phosphatidylcholine) from rat liver were isolated to model myelin/ER membranes. Anionic proteins (pI 5.65–5.80) were purified from bee venom via cation exchange chromatography. Liposomes (with/without proteins) were prepared, and proton absorption was quantified by pH changes in suspensions versus pure water. Statistical significance was assessed via ANOVA and t-tests. Results: All phospholipid liposomes examined in this study absorbed protons under the tested conditions, with phosphatidylethanolamine showing the highest capacity (pH increase: 7.00 → 7.18). Liposomes enriched with anionic proteins exhibited significantly greater proton absorption (e.g., phosphatidylserine + proteins: pH 8.15 vs. 7.15 alone; p < 2.43 × 10⁻⁶). Sphingomyelin-protein liposomes absorbed the most protons, suggesting that protein–phospholipid interactions modulate surface proton affinity. Conclusions: Anionic bee venom proteins amplify proton absorption by phospholipid membranes, supporting the hypothesis that lipid–protein complexes act as “proton capacitors”. This mechanism may underpin extramitochondrial energy storage in myelin and ER. Pharmacologically, targeting these interactions could mitigate bioenergetic deficits in aging or disease. Further research should define the structural basis of proton capture by membrane-anchored proteins. Full article

Among various H₂ production methods, splitting water using renewable electricity for H₂ production is regarded as a promising approach due to its high efficiency and zero carbon emissions. The oxygen evolution reaction (OER) is an important part of splitting water, but also the main bottleneck. The anodic oxygen evolution reaction (OER) for water electrolysis technology involves multi-electron/proton transfer and has sluggish reaction kinetics, which is the key obstacle to the overall efficiency of electrolyzing water. Therefore, it is necessary to develop highly efficient and cheap OER electrocatalysts to drive overall water splitting. Herein, a series of efficient RuO₂-Co₃O₄ composites were synthesized via a straightforward three-step process comprising solvothermal synthesis, ion exchange, and calcination. The results indicate that using 10 mg of RuCl₃·xH₂O and 15 mg of Co-MOF precursor in the second ion exchange step is the most effective way to acquire the Co₃O₄-RuO₂-10 (RCO-10) composite with the largest specific area and the best electrocatalytic performance after the calcination process. The optimal Co₃O₄-RuO₂-10 composite powder catalyst displays low overpotential (η₁₀ = 272 mV), a small Tafel slope (64.64 mV dec⁻¹), and good electrochemical stability in alkaline electrolyte; the overall performance of Co₃O₄-RuO₂-10 surpasses that of many related cobalt-based oxide catalysts. Furthermore, through integration with a carbon cloth substrate, Co₃O₄-RuO₂-10/CC can be directly used as a self-supporting electrode with high stability. This work presents a straightforward method to design Co₃O₄-RuO₂ composite array catalysts for high-performance electrocatalytic OER performance. Full article

A data set on Stark broadening parameters defining the Lorentzian line profile (spectral line widths and shifts) for 31 multiplets of four-times-charged nitrogen ion (N V), with lines broadened by impacts with electrons (e), protons (p), He II ions, $\alpha$ particles (He III), and B III, B IV, B V, and B VI ions, is given. The above-mentioned data have been calculated within the frame of the semiclassical perturbation theory, for temperatures from 50,000 K to 1,000,000 K, and densities of perturbers from 10¹⁵ cm⁻³ up to 10²¹ cm⁻³. These data are, first of all, of interest for diagnostics and modeling of laser-driven plasma in experiments and investigations of proton–boron fusion, especially when the target is boron nitride (BN). Data on Stark broadening by collisions with e, p, He II ions, and $\alpha$ particles are useful for the investigation of stellar plasma, in particular for white dwarf atmospheres and subphotospheric layer modeling. Full article

Reactive oxygen species (ROS) play a central role in photocatalytic processes relevant to environmental remediation and clean energy. This work focused on the computational investigation of ZnO surface reactivity toward H₂O and O₂ adsorption, as a preliminary step in understanding ROS generation pathways. Surface stability and adsorption energies for isolated and co-adsorbed H₂O and O₂ molecules on different ZnO surfaces (both in their pristine form and with oxygen vacancies) were evaluated using DFT calculations at the PBE-D3 level under various surface coverages. The introduction of vacancies on the pristine (001) and (100) surfaces enhanced O₂ binding, particularly in inclined configurations at the defect sites, with the adsorption energies reaching −2.63 eV and −2.04 eV, respectively. However, the (110) surface showed very strong H₂O binding, but weak O₂ adsorption, which only modestly improved with vacancies. Co-adsorption of H₂O and O₂ exhibited synergistic stabilization, especially on the (001) and (100) surfaces, where ROS were formed through proton transfers either between adsorbed H₂O and O₂ or between H₂O and surface oxygen atoms. These findings provide detailed insight into the mechanistic role of surface defects in ROS generation and support the rational design of ZnO-based photocatalysts. Full article

The cytosolic pH (pH_i) of mammalian cells is tightly maintained at values ~7.2. Cytoplasmic acidosis (pH_i < 6.8) occurs when the intracellular proton concentration ([H⁺]_i) exceeds the buffering capacity of the cytosol and transport processes to extrude protons are exhausted. During intracellular acidosis, the contractility of cardiac and skeletal muscle cells is strongly reduced, often at sufficient Ca²⁺ levels. A contraction of striated muscle is achieved when the intracellular calcium (Ca²⁺) concentration rises above resting levels. The amplitude and kinetics of Ca²⁺ signals are controlled by Ca²⁺ handling proteins and force is generated if Ca²⁺ ions interact with contractile filaments of the sarcomere. Some aspects of this phenomenon, such as the biochemical origin of excessive protons in working muscle cells and molecular interactions of protons with Ca²⁺ handling proteins or contractile filaments, are not yet fully understood. This review summarizes our current understanding of how striated muscle cells handle Ca²⁺ and H⁺ and how a rise in [H⁺]_i may interfere with Ca²⁺ signaling in the working skeletal muscle (fatigue) or during ischemic events in cardiac muscle. Finally, we briefly address experimental strategies to measure Ca²⁺ signaling at different pH values with fluorescent probes and highlight their limitations. Full article

Active galactic nuclei (AGNs) often exhibit broad-line regions (BLRs), populated by high-velocity clouds in approximately Keplerian orbits around the central supermassive black hole (SMBH) at subparsec scales. During episodes of intense accretion at super-Eddington rates, the accretion disk can launch a powerful, radiation-driven wind. This wind may overtake the BLR clouds, forming bowshocks around them. Two strong shocks arise: one propagating into the wind, and the other into the cloud. If the shocks are adiabatic, electrons and protons can be efficiently accelerated via a Fermi-type mechanism to relativistic energies. In sufficiently dense winds, the resulting high-energy photons are absorbed and reprocessed within the photosphere, while neutrinos produced in inelastic $pp$ collisions escape. In this paper, we explore the potential of super-accreting AGNs as neutrino sources. We propose a new class of neutrino emitter: an AGN lacking jets and gamma-ray counterparts, but hosting a strong, opaque, disk-driven wind. As a case study, we consider a supermassive black hole with $M_{\mathrm{BH}}={10}^6{M}_{\odot }$ and accretion rates consistent with tidal disruption events (TDEs). We compute the relevant cooling processes for the relativistic particles under such conditions and show that super-Eddington accreting SMBHs can produce detectable neutrino fluxes with only weak electromagnetic counterparts. The neutrino flux may be observable by the next-generation IceCube Observatory (IceCube-Gen2) in nearby galaxies with a high BLR cloud filling factor. For galaxies hosting more massive black holes, detection is also possible with moderate filling factors if the source is sufficiently close, or at larger distances if the filling factor is high. Our model thus provides a new and plausible scenario for high-energy extragalactic neutrino sources, where both the flux and timescale of the emission are determined by the number of clouds orbiting the black hole and the duration of the super-accreting phase. Full article

The consumption of animal- and plant-based protein food is increasing as the world population grows. Alternative protein sources that are nutritious, safe and sustainable are needed. There is a growing research interest in integrating wheat-based staple foods, such as pasta, with new ingredients that could also provide nutritional and health benefits. Despite their unquestionable nutritional value, new pasta formulations need to be evaluated in terms of technological/sensory quality. In this study, we assessed the quality and flavour of traditional egg pasta fortified with two alternative protein sources: hazelnut flour and cricket powder. It is known that a quality pasta tends to lose fewer solids during cooking. In parallel with classical evaluation of cooking and sensory characteristics, proton nuclear magnetic resonance (¹H NMR) spectroscopy of the metabolites released during the cooking process and volatile fingerprint analysis with quartz microbalance (QMB) electronic nose (E-nose) were performed. These approaches showed results complementary to those obtained from classical quality and sensory analyses, thus demonstrating the potential of ¹H NMR and E-nose in pasta quality assessment. Overall, the pasta fortification with cricket powder and hazelnut flour affected the matrix mobility by modulating the release of chemical components into the water during cooking and overcooking processes; moreover, it significantly altered the pasta sensory profile in terms of aroma and texture. This finding highlights the complexity of balancing technological improvement with sensory appeal in food product development. Full article

The state of the art in the thermodynamics of calix[4]resorcinarene derivatives and its metal ion complexes is briefly discussed in the introduction. This is followed by the synthesis and characterization of a recyclable calix[4]resorcinarene amide derivative (L). The 1H NMR analyses in CD3CN and CD3OD showed solvent-dependent conformational changes with a notable downfield chemical shift in the aromatic proton (H-2) in moving from deuterated methanol to acetonitrile, indicating an interaction of the solvent within the ligand cavity as suggested by molecular dynamic simulations. 1H NMR complexation in acetonitrile revealed that L forms relatively strong 1:1 complexes with cations, with selectivity for Ca(II) and, to lesser extent, with Pb(II) over other metal cations. The composition of the complexes is corroborated by conductance measurements. The thermodynamics of these systems indicate that the complexation process is predominantly enthalpy controlled in acetonitrile, while it is entropy controlled in methanol. A remarkable outcome of fundamental studies is found in its application as new material for the removal of Ca(II) from water. The capacity of L to remove Ca(II) from water is 24 mmol/g which exceeds by far the capacity of cation exchange resins. Full article

Most astrophysical systems (except for very compact objects such as, e.g., black holes and neutron stars) in our Universe are characterized by shallow gravitational potentials, with dimensionless compactness $|\Phi |\equiv r_s/R\ll 1$, where $r_s$ and R are their Schwarzschild radius and typical size, respectively. While the existence and characteristic scales of such virialized systems depend on gravity, we demonstrate that the value of $|\Phi |$—and thus the non-relativistic nature of most astrophysical objects—arises from microphysical parameters, specifically the fine structure constant and the electron-to-proton mass ratio, and is fundamentally independent of the gravitational constant, G. In fact, the (generally extensive) gravitational potential becomes ‘locally’ intensive at the system boundary; the compactness parameter corresponds to the binding energy (or degeneracy energy, in the case of quantum degeneracy pressure-supported systems) per proton, representing the amount of work that needs to be done in order to allow proton extraction from the system. More generally, extensive properties of gravitating systems depend on G, whereas intensive properties do not. It then follows that peak rms values of large-scale astrophysical velocities and escape velocities associated with naturally formed astrophysical systems are determined by electromagnetic and atomic physics, not by gravitation, and that the compactness, $|\Phi |$, is always set by microphysical scales—even for the most compact objects, such as neutron stars, where $|\Phi |$ is determined by quantities like the pion-to-proton mass ratio. This observation, largely overlooked in the literature, explains why the Universe is not dominated by relativistic, compact objects and connects the relatively low entropy of the observable Universe to underlying basic microphysics. Our results emphasize the central but underappreciated role played by dimensionless microphysical constants in shaping the macroscopic gravitational landscape of the Universe. In particular, we clarify that this independence of the compactness, $|\Phi |$, from G applies specifically to entire, virialized, or degeneracy pressure-supported systems, naturally formed astrophysical systems—such as stars, galaxies, and planets—that have reached equilibrium between self-gravity and microphysical processes. In contrast, arbitrary subsystems (e.g., a piece cut from a planet) do not exhibit this property; well within/outside the gravitating object, the rms velocity is suppressed and G reappears. Finally, we point out that a clear distinction between intensive and extensive astrophysical/cosmological properties could potentially shed new light on the mass hierarchy and the cosmological constant problems; both may be related to the large complexity of our Universe. Full article

Objectives: This study aimed to evaluate the usefulness of a short magnetic resonance (MR) protocol for knee evaluation, using 3D PDW SPAIR sequences compared with traditional 2D ones. Methods: A prospective analysis included 76 patients with knee pain. MR was performed using a 1.5 T scanner. The standard protocol consisted of multiplanar 2D proton density-weighted (PDW) SPectral Attenuated Inversion Recovery (SPAIR) and additional T1-weighted (T1W) and T2-weighted (T2W) sequences, with a total acquisition time of 17 min. The simulated short protocol included a 3D PDW SPAIR sequence with isotropic voxels and a slice thickness of 0.6 mm, coronal T1W, and gradient echo (GRE) axial sequences, with a total acquisition time of 9 min. Two radiologists independently reviewed images and collected pathological processes. Results: The 3D PDW SPAIR sequence demonstrated a significantly higher subjective image quality compared to standard 2D sequences [κ = 0.712 (p < 0.001) vs. κ = 0.144 (p = 0.63); p < 0.001]. Artifacts were not significantly different between the two protocols (p = 0.201). Qualitative assessments showed superior ratings for 3D images (excellent quality: 72.4% vs. 26.3% for 3D and 2D, respectively; p < 0.001). Diagnostic performance was comparable between the two protocols for ACL injuries, medial and lateral collateral ligament injuries, and chondropathies. Three-dimensional sequences were more effective in detecting medial meniscal injuries (p < 0.001), particularly radial and complex tears, likely due to higher spatial resolution and multiplanar reconstruction capability. Conclusions: The 3D PDW SPAIR sequence offers advantages in knee MRI study, including improved image quality, reduced acquisition time, and superior detection of meniscal injuries. Full article

The role of p-benzoquinone (BQ) as a photocatalyst in the synthesis of solketal under UV irradiation has been studied, along with the combined use of BQ/TiO₂ P25 as a photocatalytic system for the process. The presence of the O₂/O₂^−• redox couple is essential for the reaction to take place. However, experiments with p-benzoquinone as a superoxide radical scavenger failed, with the opposite effect of enhancing the reaction being observed. It was found that p-benzoquinone and oxygen compete for photogenerated electrons in the conduction band of titania. A redox equilibrium between p-benzoquinone and hydroquinone (H₂Q), mediated by the O₂/O₂^−• system, was identified as a key factor in enabling the reaction. Furthermore, EPR spin-trapping experiments confirmed the presence of the carbon-centered radical 2-hydroxypropan-2-yl, which was determined to be the main radical species involved in the process. Either acetone or 2-propanol can generate this radical, with the BQ/H₂Q redox system being pivotal in the formation of the hemiacetal intermediate. This intermediate is subsequently converted into the final acetal (solketal), with H₂Q acting as a photoacid through an excited-state proton transfer (ESPT) mechanism. The photoacid behavior of hydroquinone was confirmed using pyridine as a basic probe, as the formation of hydroquinone–pyridine adducts was detected by Raman spectroscopy. Full article

This study proposes a MPL (microporous layer)–GDL (gas diffusion layer) microstructure reconstruction method based on a novel random reconstruction algorithm. Then the Shan–Chen multi-component and multi-phase lattice Boltzmann method (SC-LBM) is used to systematically describe the influence of different contact angle distributions on the drainage characteristics of the GDL of proton exchange membrane fuel cells (PEMFCs). Meanwhile, the breakthrough time of liquid water, steady-state time, and liquid water saturation are compared. The results show that with the increase in contact angle, the time for the first droplet breakthrough and the steady-state time are significantly shortened, and the saturation of liquid water gradually decreases at the steady state, indicating that increasing hydrophobicity can effectively improve the drainage capacity of the GDL. Several double-gradient and three-gradient contact angle distribution schemes are studied, and it is found that the gradient structure with increasing contact angles along the direction of water flow will lead to prolonged steady-state time and elevated water saturation, which is not conducive to drainage. This study analyzes the drainage process under different wettability gradients considering aspects such as the droplet morphology evolution, flow path, and water distribution mechanism, clarifying the key role of gradient design in GDL water management. This work also provides a theoretical basis and design guidelines for wettability optimization in the GDL of PEMFCs. Full article

This study assesses how different anode materials influence neutron production rates (NPRs) in multi-state fusion (MSF) reactors, with a particular focus on the effects of deuterium (D) pre-loading on the anode surface. Three types of mesh anodes were assessed: stainless steel (SS), zirconium (Zr), and D pre-loaded zirconium (ZrD). MSF operates using two electrodes to confine ions to various fusion reactions, including D-D and D-T. The reactor features a negatively biased central cathode and a grounded anode within a vacuum vessel. Neutrons and protons are produced through the application of high voltage (tens of kV) and current (tens of mA) on the system to spark the plasma and start the fusion. Assessments at voltages up to 50 kV and currents up to 30 mA showed that Zr mesh anodes produced higher NPRs than SS ones, reaching 1.912 at 30 kV. This increased performance is attributed to surface fusion processes occurring in the anode. These processes were further modified by the deuterium pre-loading in the ZrD anode, as compared to SS and Zr with 1.832 at 30 kV. The findings suggest that material properties and deuterium pre-loading play significant roles in optimizing the efficiency of MSF reactors and the NPR. Future research may explore the long-term stability and durability of these anode materials under continuous operation conditions to fully harness their potential in fusion energy applications. Full article

In high energy density physics, the demand for precise detection of nanosecond-level fast physical processes is high. Ga:ZnO (GZO), GaN, and other fast scintillators are widely used in pulsed signal detection. However, many of them, especially wide-bandgap materials, still face issues of low luminous intensity and significant self-absorption. Therefore, an enhanced method was proposed to tune the wavelength of materials via coating perovskite quantum dot (QD) films. Three-layer samples based on GZO were primarily investigated and characterized. Radioluminescence (RL) spectra from each face of the samples, as well as their decay times, were obtained. Lower temperatures further enhanced the luminous intensity of the samples. Its overall luminous intensity increased by 2.7 times at 60 K compared to room temperature. The changes in the RL processes caused by perovskite QD and low temperatures were discussed using the light tuning and transporting model. In addition, an experiment under a pico-second electron beam was conducted to verify their pulse response and decay time. Accordingly, the samples were successfully applied in beam state monitoring of nanosecond pulsed proton beams, which indicates that GZO wafer coating with perovskite QD films has broad application prospects in pulsed radiation detection. Full article

One of the advantages of the EPR spectroscopy method in assessing structural defects caused by irradiation is the fact that using this method it is possible to determine not only the concentration dependences of the defect structure but to also establish their type, which is not possible with methods such as X-ray diffraction or scanning electron microscopy. Based on the data obtained, the role of variation in the ratio of components in Li₄SiO₄–Li₂TiO₃ ceramics on the processes of softening under high-dose irradiation with protons simulating the accumulation of hydrogen in the damaged layer, as well as the concentration of structural defects in the form of oxygen vacancies and radiolysis products on the processes of high-temperature degradation of ceramics, was determined. It was found that the main changes in the defect structure during the prolonged thermal exposure of irradiated samples are associated with the accumulation of oxygen vacancies, the density of which was estimated by the change in the intensity of singlet lithium, characterizing the presence of E-centers. At the same time, it was found that the formation of interphase boundaries in the structure of Li₄SiO₄–Li₂TiO₃ ceramics leads to the inhibition of high-temperature degradation processes in the case of post-radiation thermal exposure for a long time. Also, during the conducted studies, the role of thermal effects on the structural damage accumulation rate in Li₄SiO₄–Li₂TiO₃ ceramics was determined in the case when irradiation is carried out at different temperatures. During the experiments, it was determined that the main contribution of thermal action in the process of proton irradiation at a fluence of 5 × 10¹⁷ proton/cm² is an increase in the concentration of radiolysis products, described by changes in the intensities of spectral maxima, characterized by the presence of defects such as ≡Si–O, SiO₄³⁻ and Ti³⁺ defects. Full article