Search Results (30,915)

Background/Objectives: Antimicrobial resistance (AMR) contributes to millions of deaths globally each year, creating an urgent need for new therapeutic agents. Antimicrobial peptides (AMPs) have emerged as promising candidates due to their potential to combat AMR pathogens. This study aimed to evaluate the antimicrobial activity of an AMP from a soil-derived bacterial isolate against Gram-negative bacteria. Method: Soil bacteria were isolated and screened for antimicrobial activity. The bioactive peptide was purified and determined its structure and antimicrobial efficacy. Genomic analysis was conducted to predict the biosynthetic gene clusters (BGCs) responsible for AMP production. Results: Genomic analysis identified the isolate as Paenibacillus sp. Na14, which exhibited low genomic similarity (61.0%) to other known Paenibacillus species, suggesting it may represent a novel species. The AMP from the Na14 strain exhibited heat stability up to 90 °C for 3 h and retained its activity across a broad pH range from 3 to 11. Structural analysis revealed that the Na14 peptide consisted of 14 amino acid residues, adopting an α-helical structure. This peptide exhibited bactericidal activity at concentrations of 2–4 µg/mL within 6–12 h, and its killing rate was concentration-dependent. The peptide was found to disrupt the bacterial membranes. The Na14 peptide shared 64.29% sequence similarity with brevibacillin 2V, an AMP from Brevibacillus sp., which also belongs to the Paenibacillaceae family. Genomic annotation identified BGCs associated with secondary metabolism, with a particular focus on non-ribosomal peptide synthetase (NRPS) gene clusters. Structural modeling of the predicted NRPS enzymes showed high similarity to known NRPS modules in Brevibacillus species. These genomic findings provide evidence supporting the similarity between the Na14 peptide and brevibacillin 2V. Conclusions: This study highlights the discovery of a novel AMP with potent activity against Gram-negative pathogens and provides new insight into conserved AMP biosynthetic enzymes within the Paenibacillaceae family. Full article

Carbon fiber-reinforced polymer (CFRP) composites are widely used in aerospace due to their excellent strength-to-weight ratio and tailorable properties. However, these properties critically depend on the CFRP curing cycle. The commonly adopted manufacturer-recommended curing cycle (MRCC), designed to accommodate the most conservative conditions, involves prolonged curing times and high energy consumption. To overcome these limitations, this study proposes an efficient and adaptable method to determine the optimal curing cycle. The effects of varying heating rates on resin dynamic and isothermal–exothermic behavior were characterized via reaction kinetics analysis using differential scanning calorimetry (DSC) and rheological measurements. The activation energy of the reaction system was substituted into the modified Sun–Gang model, and the parameters were estimated using a particle swarm optimization algorithm. Based on the curing kinetic behavior of the resin, CFRP compression molding process orthogonal experiments were conducted. A weighted scoring system incorporating strength, energy consumption, and cycle time enabled multidimensional evaluation of optimized solutions. Applying this curing cycle optimization method to a commercial epoxy resin increased efficiency by 247.22% and reduced energy consumption by 35.7% while meeting general product performance requirements. These results confirm the method’s reliability and its significance for improving production efficiency. Full article

The cement industry is a significant contributor to global carbon dioxide emissions, primarily due to the energy demands of its production process and its reliance on clinker, a material formed through the high-temperature calcination of limestone. Strategies to reduce emissions include the adoption of low-carbon fuels, the use of carbon capture and storage (CCS) technologies, and the integration of supplementary cementitious materials (SCMs) to reduce the clinker content. The effectiveness of these measures depends on a complex set of interactions involving technological feasibility, market dynamics, and regulatory frameworks. This study presents a system dynamics model designed to assess how various decarbonization approaches influence long-term emission trends within the cement industry. The model accounts for supply chains, production technologies, market adoption rates, and changes in cement production costs. This study then analyzes a number of scenarios where there is large-scale sustained investment in each of three carbon mitigation strategies. The results show that CCS by itself allows the cement industry to achieve carbon neutrality, but the high capital investment results in a large cost increase for cement. A combined approach using alternative fuels and SCMs was found to achieve a large carbon reduction without a sustained increase in cement prices, highlighting the trade-offs between cost, effectiveness, and system-wide interactions. Full article

Parabens—specifically methylparaben (MeP), ethylparaben (EtP), propylparaben (PrP), and butylparaben (BuP)—are widely used substances in everyday life, particularly as preservatives in pharmaceutical and food products. However, these compounds are not effectively removed by conventional water and wastewater treatment processes, potentially causing disruptions to human homeostasis and the endocrine system. This study conducted a transport and dimensional analysis through simulation of the adsorption process for these parabens, using zinc chloride-activated carbon derived from spent coffee grounds (ACZnCl₂) as the adsorbent, implemented via Aspen Properties^® and Aspen Adsorption^®. Simulations were performed for two inlet concentrations (50 mg/L and 100 mg/L) and two adsorption column heights (3 m and 4 m), considering a volumetric flow rate representative of a medium-sized city with approximately 100,000 inhabitants. The results showed that both density and surface tension of the parabens varied linearly with increasing temperature, and viscosity exhibited a marked reduction above 30 °C. Among the tested conditions, the configuration with 50 mg∙L⁻¹ inlet concentration and a 4 m column height demonstrated the highest adsorption capacity and better performance under adsorption–desorption equilibrium. These findings indicate that the implementation of adsorption beds on an industrial scale in water and wastewater treatment systems is both environmentally and socially viable. Full article

Unemployment and weak economic productivity are significant global issues, particularly in West Africa. Recently, through diverse mechanisms, remittances and foreign direct investment (FDI) have been sources of foreign capital flow that have positively influenced many less developed economies, including ECOWAS (ECOWAS stands for Economic Community of West African States). Nevertheless, these financial flows have exhibited significant inconsistencies, primarily resulting from economic downturns in migrants’ destination countries, with remarkable implications for beneficiary economies. This study, therefore, examines the effect of remittances and FDI on employment in ECOWAS. Specifically, the study assesses the effects of the inflow of remittances and FDI on employment using panel dynamic ordinary least squares (PDOLS) and also investigates the shock effects of remittances and FDI by employing Panel Vector Error Correction (PVECM), which involves variance decomposition. The results show that foreign direct investment (FDI) positively and significantly affects employment. Other variables that show a significant relationship with employment are wage rate, education expenditure, and interest rate. The variance decomposition result revealed that external shocks on remittances and FDI have short- and long-term effects on employment. The above findings imply that foreign direct investment has a far-reaching positive impact on the economy-wide management of the West African sub-region and thus calls for relevant policy options. Full article

High-precision 6D pose estimation for pick-and-place operations remains a critical problem for industrial robot arms in manufacturing. This study introduces an analytics-based solution for 6D pose estimation designed for a real-world industrial application: it enables the Staubli TX2-60L (manufactured by Stäubli International AG, Horgen, Switzerland) robot arm to pick up metal plates from various locations and place them into a precisely defined slot on a brake pad production line. The system uses a fixed eye-to-hand Intel RealSense D435 RGB-D camera (manufactured by Intel Corporation, Santa Clara, California, USA) to capture color and depth data. A robust software infrastructure developed in LabVIEW (ver.2019) integrated with the NI Vision (ver.2019) library processes the images through a series of steps, including particle filtering, equalization, and pattern matching, to determine the X-Y positions and Z-axis rotation of the object. The Z-position of the object is calculated from the camera’s intensity data, while the remaining X-Y rotation angles are determined using the angle-of-inclination analytics method. It is experimentally verified that the proposed analytical solution outperforms the hybrid-based method (YOLO-v8 combined with PnP/RANSAC algorithms). Experimental results across four distinct picking scenarios demonstrate the proposed solution’s superior accuracy, with position errors under 2 mm, orientation errors below 1°, and a perfect success rate in pick-and-place tasks. Full article

Subcooling-based ice slurry production faces challenges in terms of energy efficiency and operational stability, which limit its applications for large-scale cold energy storage. A thermodynamic model is established to investigate the effects of key control parameters, including evaporation temperature, condensation temperature, subcooling degree, water flow rate, type of refrigerant, and adiabatic compression efficiency. The results show that using the refrigerant R161 achieves the highest energy efficiency, indicating that R161 is the optimal refrigerant in this research. When the evaporation and condensation temperatures are −10 °C and 30 °C, respectively, the system achieves the maximum comprehensive performance coefficient of 2.43. Moreover, under a flow velocity of 0.8 m/s and a temperature of 0.5 °C, the system achieves a peak ice production rate of 45.28 kg/h. A high water temperature and high flow velocity would significantly degrade the system’s ice production capacity. This research provides useful guidance for the design, optimization, and application of ice slurry-based cold energy storage systems. Full article

Chemical fertilizers pose significant risks to both human health and the environment. To investigate the effect of substituting nitrogen fertilizer with vermicompost tea on wheat yield, shoot chemical constituents, and grain quality under clay-loam soil conditions, two field experiments were conducted at the Faculty of Agriculture, Cairo University, Egypt, during the winter seasons of 2021–2022 and 2022–2023. A split-plot design in randomized complete blocks with three replications was employed. Vermicompost tea was assigned to the main plots, while wheat cultivars were assigned to the subplots. The cultivars were evaluated under four treatments involving partial substitution of mineral nitrogen (recommended dose of nitrogen (RDN%, 190 kg N ha⁻¹): a control (90% of RDN + 25 kg vermicompost tea), 80% of RDN + 37.5 kg vermicompost tea, and 70% of RDN + 50 kg vermicompost tea. Nitrogen fertilizer (RDN%) was applied at rates of 190 (control), 170 (90%), 150 (80%), and 130 (70%) kg N ha⁻¹. The results indicated that partially substituting mineral nitrogen with vermicompost tea significantly increased grain weight/Ha, chlorophyll A, chlorophyll B, carotenoids, nitrogen, phosphorus (P), and potassium (K) content in shoots, as well as ash, crude protein, crude fiber, total sugar, and N, P, and K content in wheat grains. The grain weight/Ha of the Sakha-95, Giza-171, and Sads-14 cultivars increased by 38.6%, 33.5%, and 39.3%, respectively, when treated with 70% RDN + 50 kg vermicompost tea. The combination of the Sads-14 cultivar and 70% RDN + 50 kg vermicompost tea resulted in the highest values for grain weight/ha (9.43 tons ha⁻¹), chlorophyll A (1.39 mg/g), chlorophyll B (1.04 mg/g), N (5.08%), P (1.63%), and P (2.43%) content in shoots. The same combination also improved ash (2.89%), crude fiber (2.84%), and K (6.05%) content in grains. In conclusion, the application of vermicompost tea in conjunction with chemical fertilizers offers a viable alternative to using chemical fertilizers alone, promoting sustainable agricultural practices and improving wheat production. It is recommended that mineral nitrogen fertilizer be partially replaced with vermicompost tea to enhance both the productivity and grain quality of wheat while minimizing environmental pollution. Full article

We present a framework that aims to investigate the role of thermal fluctuations in matter composition and color superconductivity in the nucleation of three-flavor deconfined quark matter in the typical conditions of high-energy astrophysical systems related to compact stars. It is usually assumed that the flavor composition is locally fixed during the formation of the first seed of deconfined quark matter, since a weak interaction acts too slowly to re-equilibrate flavors. However, the matter composition fluctuates around its average equilibrium values at the typical temperatures of high-energy astrophysical processes. Here, we extend our previous two-flavor nucleation formalism to a three-flavor case. We develop a thermodynamic framework incorporating finite-size effects and thermal fluctuations in the local composition to compute the nucleation probability as the product of droplet formation and composition fluctuation rates. Moreover, we discuss the role of color superconductivity in nucleation, arguing that it can play a role only in systems larger than the typical coherence length of diquark pairs. We found that thermal fluctuations in the matter composition led to lowering the potential barrier between the metastable hadronic phase and the stable quark phase. Moreover, the formation of diquark pairs reduced the critical radius and thus the potential barrier in the low baryon density and temperature regime. Full article

To investigate the spatiotemporal evolution characteristics and primary driving factors of Gross Primary Productivity (GPP) on the Qinghai-Tibet Plateau, we employed an enhanced MODIS-PSN model. Utilizing the fifth-generation global climate reanalysis dataset (ECMWF ERA5), we generated GPP remote sensing products by integrating six natural factors. Through correlation analysis and geographical detector modeling, we quantitatively analyzed the spatiotemporal dynamics and key drivers of vegetation GPP across the Qinghai-Tibet Plateau from 2001 to 2022. The results demonstrate that GPP changes across the Qinghai-Tibet Plateau display pronounced spatial heterogeneity. The humid northeastern and southeastern regions exhibit significantly positive change rates, primarily distributed across wetland and forest ecosystems, with a maximum mean annual change rate of 12.40 gC/m²/year. In contrast, the central and southern regions display a decreasing trend, with the minimum change rate reaching −1.61 gC/m²/year, predominantly concentrated in alpine grasslands and desert areas. Vegetation GPP on the Qinghai-Tibet Plateau shows significant correlations with temperature, vapor pressure deficit (VPD), evapotranspiration (ET), leaf area index (LAI), precipitation, and radiation. Among the factors analyzed, LAI demonstrates the strongest explanatory power for spatial variations in vegetation GPP across the Qinghai-Tibet Plateau. The dominant factors influencing vegetation GPP on the Qinghai-Tibet Plateau are LAI, ET, and precipitation. The pairwise interactions between these factors exhibit linear enhancement effects, demonstrating synergistic multifactor interactions. This study systematically analyzed the response mechanisms and variations of vegetation GPP to multiple driving factors across the Qinghai-Tibet Plateau from a spatial heterogeneity perspective. The findings provide both a critical theoretical framework and practical insights for better understanding ecosystem response dynamics and drought conditions on the plateau. Full article

Diploid Vaccinium fuscatum is a wild blueberry species with a low chilling requirement, an evergreen growth habit, and soil adaptability to southeast US growing regions. Regardless of its potential to improve the abiotic and biotic resilience of cultivated blueberries, this species has rarely been used for blueberry breeding. One hurdle is the ploidy barrier between diploid V. fuscatum and tetraploid cultivated highbush blueberries. To overcome the ploidy barrier, vegetative shoots micro-propagated from one genotype of V. fuscatum, selected because it grew vigorously in vitro and two southern highbush cultivars, ‘Emerald’ and ‘Rebel,’ were treated with colchicine. While shoot regeneration was severely repressed in ‘Emerald’ and ‘Rebel,’ shoot production from the V. fuscatum clone was not compromised at either 500 µM or 5000 µM colchicine concentrations. Due to the high number of shoots produced in vitro via the V. fuscatum clone shoots of this clone that had an enlarged stem diameter in vitro were subjected to flow cytometer analysis to screen for induced polyploidy. Sixteen synthetic tetraploid V. fuscatum, one synthetic octoploid ‘Emerald,’ and three synthetic octoploid ‘Rebel’ were identified. Growth rates of the polyploid-induced mutants were reduced compared to their respective wildtype controls. The leaf width and length of synthetic tetraploid V. fuscatum and synthetic octoploid ‘Emerald’ was increased compared to the wildtypes, whereas the leaf width and length of synthetic octoploid ‘Rebel’ were reduced compared to the wildtype controls. Significant increases in stem thickness and stomata guard cell length were found in the polyploidy-induced mutant lines compared to the wildtypes. In the meantime, stomata density was reduced in the mutant lines. These morphological changes may improve drought tolerance and photosynthesis in these mutant lines. Synthetic tetraploid V. fuscatum can be used for interspecific hybridization with highbush blueberries to expand the genetic base of cultivated blueberries. Full article

In China’s Loess Plateau, sustainable agricultural end products are affected by an insufficiency of water resources. Rising crop water use efficiency (WUE) through field management pattern improvement is a crucial plan of action to address this issue. However, there is no agreement among researchers on the most appropriate field management practices regarding WUE, which requires further integrated quantitative analysis. We conducted a meta-analysis by quantifying the effect of agricultural practices surrounding nitrogen (N) fertilizer management. The two experimental cultivars were Yunhan–20410 and Yunhan–618. The subplots included nitrogen 0 kg·ha⁻¹ (N0), 90 kg·ha⁻¹ (N90), 180 kg·ha⁻¹ (N180), 210 kg·ha⁻¹ (N210), and 240 kg·ha⁻¹ (N240). Our results show that higher N rates (up to N210) enhanced water consumption during the node-flowering and flowering-maturity time periods. YH–618 showed higher water use during the sowing–greening and node-flowering periods but decreased use during the greening-node and flowering-maturity periods compared to YH–20410. The N210 treatment under YH–618 maximized water use efficiency (WUE). Increased N rates (N180–N210) decreased covering temperatures (T_max, T_min, T_aver) during flowering, increasing the level of grain filling. Spike numbers rose with N application, with an off-peak at N210 for strong-gluten wheat. The 1000-grain weight was at first enhanced but decreased at the far end of N180–N210. YH–618 with N210 achieved a harvest index (HI) similar to that of YH–20410 with N180, while excessive N (N240) or water reduced the HI. Dry matter accumulation increased up to N210, resulting in earlier stabilization. Soil water consumption from wintering to jointing was strongly correlated with pre-flowering dry matter biological process and yield, while jointing–flowering water use was linked to post-flowering dry matter and spike numbers. Post-flowering dry matter accumulation was critical for yield, whereas spike numbers positively impacted yield but negatively affected 1000-grain weight. In conclusion, our results provide evidence for determining suitable integrated agricultural establishment strategies to ensure efficient water use and sustainable production in the Loess Plateau region. Full article

Herein, α-MnO₂-supported Pt-Pd bimetal (xPd-yPt/α-MnO₂; x and y are the weight loadings (wt%) of Pd and Pt, respectively; x = 0, 0.23, 0.47, 0.93, and 0.92 wt%; and y = 0.91, 0.21, 0.46, 0.89, and 0 wt%) catalysts were prepared using the polyvinyl alcohol-protected NaBH₄ reduction method. The physicochemical properties of the catalysts were determined by means of various techniques and their catalytic activities for toluene oxidation were evaluated. It was found that among the xPd-yPt/α-MnO₂ samples, 0.93Pd-0.89Pt/α-MnO₂ showed the best catalytic performance, with the toluene oxidation rate at 156 °C (r_cat) and space velocity = 60,000 mL/(g h) being 6.34 × 10⁻⁴ mol/(g s), much higher than that of 0.91Pt/α-MnO₂ (1.31 × 10⁻⁴ mol/(g s)) and that of 0.92Pd/α-MnO₂ (6.13 × 10⁻⁵ mol/(g s)) at the same temperature. The supported Pd-Pt bimetallic catalysts possessed higher Mn³⁺/Mn⁴⁺ and O_ads/O_latt molar ratios, which favored the enhancement in catalytic activity of the supported Pd-Pt bimetallic catalysts. Furthermore, the 0.47Pd-0.46Pt/α-MnO₂ sample showed better resistance to sulfur dioxide poisoning. The partial deactivation of 0.47Pd-0.46Pt/α-MnO₂ was attributed to the formation of sulfate species on the sample surface, which covered the active site of the sample, thus decreasing its toluene oxidation activity. In addition, the in situ DRIFTS results demonstrated that benzaldehyde and benzoate were the intermediate products of toluene oxidation. Full article

Wellington, New Zealand, is highly vulnerable to disaster-induced food security crises due to its geography and geological characteristics, which can disrupt transportation and isolate the city following disasters. Urban agriculture (UA) has been proposed as a potential alternative food source for post-disaster scenarios. This study examined the potential of urban agriculture for enhancing post-disaster food security by calculating vegetable self-sufficiency rates. Specifically, it evaluated the capacity of current Wellington’s community gardens to meet post-disaster vegetable demand in terms of both weight and nutrient content. Data collection employed mixed methods with questionnaires, on-site observations and mapping, and collecting high-resolution aerial imagery. Garden yields were estimated using self-reported data supported by literature benchmarks, while cultivated areas were quantified through on-site mapping and aerial imagery analysis. Six post-disaster food demand scenarios were used based on different target populations to develop an understanding of the range of potential produce yields. Weight-based results show that community gardens currently supply only 0.42% of the vegetable demand for residents living within a five-minute walk. This rate increased to 2.07% when specifically targeting only vulnerable populations, and up to 10.41% when focusing on gardeners’ own households. However, at the city-wide level, the current capacity of community gardens to provide enough produce to feed people remained limited. Nutrient-based self-sufficiency was lower than weight-based results; however, nutrient intake is particularly critical for vulnerable populations after disasters, underscoring the greater challenge of ensuring adequate nutrition through current urban food production. Beyond self-sufficiency, this study also addressed the role of UA in promoting food diversity and acceptability, as well as its social and psychological benefits based on the questionnaires and on-site observations. The findings indicate that community gardens contribute meaningfully to post-disaster food security for gardeners and nearby residents, particularly for vulnerable groups with elevated nutritional needs. Despite the current limited capacity of community gardens to provide enough produce to feed residents, findings suggest that Wellington could enhance post-disaster food self-reliance by diversifying UA types and optimizing land-use to increase food production during and after a disaster. Realizing this potential will require strategic interventions, including supportive policies, a conducive social environment, and diversification—such as the including private yards—all aimed at improving food access, availability, and nutritional quality during crises. The primary limitation of this study is the lack of comprehensive data on urban agriculture in Wellington and the wider New Zealand context. Addressing this data gap should be a key focus for future research to enable more robust assessments and evidence-based planning. Full article

Early weaning of piglets elicits weaning stress, which in turn induces oxidative stress and consequently impairs growth and development. Hydrogen-rich water (HRW), characterized by selective antioxidant properties, mitigates oxidative stress damage and serves as an ideal intervention. This study aimed to evaluate the effects of HRW on weaned piglets, specifically investigating its impact on growth performance, diarrhea incidence, antioxidant function, intestinal morphology, gut microbiota, and hepatic metabolites. The results demonstrate that HRW significantly increased the average daily feed intake and significantly reduced the diarrhea rate in weaned piglets. Analysis of serum oxidative stress indicators revealed that HRW significantly elevated the activities of total antioxidant capacity and total superoxide dismutase while significantly decreasing malondialdehyde concentration. Assessment of intestinal morphology showed that HRW significantly increased the villus height to crypt depth ratio in the duodenum, jejunum, and ileum. Microbial analysis indicated that HRW significantly increased the abundance of Prevotella in the colon. Furthermore, HRW increased the abundance of beneficial bacteria, such as Akkermansia, in the jejunum and cecum, while concurrently reducing the abundance of harmful bacteria like Escherichia. Hepatic metabolite profiling revealed that HRW significantly altered the metabolite composition in the liver of weaned piglets. Differentially abundant metabolites were enriched in oxidative stress-related KEGG pathways, including ABC transporters; pyruvate metabolism; autophagy; FoxO signaling pathway; glutathione metabolism; ferroptosis; and AMPK signaling pathways. In conclusion, HRW alleviates diarrhea and promotes growth in weaned piglets by enhancing antioxidant capacity. These findings provide a scientific foundation for the application of HRW in swine production and serve as a reference for further exploration into the mechanisms underlying HRW’s effects on animal health and productivity. Full article