Search Results (582)

E-commerce growth has reshaped consumer behavior and retail services, driving parcel demand and challenging last-mile logistics. Existing research predominantly relies on survey data and global regression models that overlook intra-urban spatial heterogeneity in shopping behaviors. This study bridges this gap by analyzing e-commerce demand’s spatial distribution from a retail service perspective, identifying key drivers, and evaluating implications for omnichannel strategies and logistics. Utilizing waybill big data, spatial analysis, and multiscale geographically weighted regression, we reveal: (1) High-density e-commerce demand areas are predominantly located in central districts, whereas peripheral regions exhibit statistically lower volumes. The spatial distribution pattern of e-commerce demand aligns with the urban development spatial structure. (2) Factors such as population density and education levels significantly influence e-commerce demand. (3) Convenience stores play a dual role as retail service providers and parcel collection points, reinforcing their importance in shaping consumer accessibility and service efficiency, particularly in underserved urban areas. (4) Supermarkets exert a substitution effect on online shopping by offering immediate product availability, highlighting their role in shaping consumer purchasing preferences and retail service strategies. These findings contribute to retail and consumer services research by demonstrating how spatial e-commerce demand patterns reflect consumer shopping preferences, the role of omnichannel retail strategies, and the competitive dynamics between e-commerce and physical retail formats. Full article

A novel cellulose-degrading bacterial strain, D3-1, capable of degrading cellulose under medium- to high-temperature conditions, was isolated from soil samples and identified as Staphylococcus caprae through 16SrRNA gene sequencing. The strain’s cellulase production was optimized by controlling different factors, such as pH, temperature, incubation period, substrate concentration, nitrogen and carbon sources, and response surface methods. The results indicated that the optimal conditions for maximum cellulase activity were an incubation time of 91.7 h, a temperature of 41.8 °C, and a pH of 4.9, which resulted in a maximum cellulase activity of 16.67 U/mL, representing a 165% increase compared to pre-optimization levels. The above experiment showed that, when maize straw flour was utilized as a natural carbon source, strain D3-1 exhibited relatively high cellulase production. Furthermore, gas chromatography–mass spectrometry (GC-MS) analysis of products in the degradation liquid revealed the presence of primary sugars. The results indicated that, in the denitrification of simulated sewage, supplying maize straw flour degradation liquid (MSFDL) as the carbon source resulted in a carbon/nitrogen (C/N) ratio of 6:1 after a 24 h reaction with the denitrifying strain WH-01. The total nitrogen (TN) reduction was approximately 70 mg/L, which is equivalent to the removal efficiency observed in the glucose-fed denitrification process. Meanwhile, during a 4 h denitrification reaction in urban sewage without any denitrifying bacteria, but with MSFDL supplied as the carbon source, the TN removal efficiency reached 11 mg/L, which is approximately 70% of the efficiency of the glucose-fed denitrification process. Furthermore, experimental results revealed that strain D3-1 exhibits some capacity for nitrogen removal; when the cellulose-degrading strain D3-1 is combined with the denitrifying strain WH-01, the resulting TN removal rate surpasses that of a single denitrifying bacterium. In conclusion, as a carbon source in municipal sewage treatment, the degraded maize straw flour produced by strain D3-1 holds potential as a substitute for the glucose carbon source, and strain D3-1 has a synergistic effect with the denitrifying strain WH-01 on TN elimination. Thus, this research offers new insights and directions for advancement in environmental sewage treatment. Full article

Tissue engineering enables autologous reconstruction of human tissues, addressing limitations in tissue availability and immune compatibility. Several tissue engineering techniques, such as self-assembly, rely on or benefit from extracellular matrix (ECM) secretion by fibroblasts to produce biomimetic scaffolds. Models have been developed for use in humans, such as skin and corneas. Ascorbic acid (vitamin C, AA) is essential for collagen biosynthesis. However, AA is chemically unstable in culture, with a half-life of 24 h, requiring freshly prepared AA with each change of medium. This study aims to demonstrate the functional equivalence of 2-phospho-L-ascorbate (2PAA), a stable form of AA, for tissue reconstruction. Dermal, vaginal, and bladder stroma were reconstructed by self-assembly using tissue-specific protocols. The tissues were cultured in a medium supplemented with either freshly prepared or frozen AA, or with 2PAA. Biochemical analyses were performed on the tissues to evaluate cell density and tissue composition, including collagen secretion and deposition. Histology and quantitative polarized light microscopy were used to evaluate tissue architecture, and mechanical evaluation was performed both by tensiometry and atomic force microscopy (AFM) to evaluate its macroscopic and cell-scale mechanical properties. The tissues produced by the three ascorbate conditions had similar collagen deposition, architecture, and mechanical properties in each organ-specific stroma. Mechanical characterization revealed tissue-specific differences, with tensile modulus values ranging from 1–5 MPa and AFM-derived apparent stiffness in the 1–2 kPa range, reflecting the nonlinear and scale-dependent behavior of the engineered stroma. The results demonstrate the possibility of substituting AA with 2PAA for tissue engineering. This protocol could significantly reduce the costs associated with tissue production by reducing preparation time and use of materials. This is a crucial factor for any scale-up activity. Full article

The capitula of Chrysanthemum indicum Linné or C. morifolium Ramatuelle (Kikuka in Japanese) are included in several formulae of Kampo medicines (traditional Japanese medicines), such as Chotosan, which is used for headache and dizziness. Luteolin, the principal constituent of C. indicum, has antioxidant and anti-inflammatory activities. However, the effects of other flavonoids on this crude drug have not yet been thoroughly investigated. To evaluate and compare anti-inflammatory effects, we used primary cultured rat hepatocytes, which produce proinflammatory mediators, such as nitric oxide (NO) and proinflammatory cytokines, in response to interleukin (IL)-1β. Eight derivatives of 5,7-dihydroxyflavone were purified and identified in the ethyl acetate-soluble fraction of a C. indicum capitulum extract: luteolin (Compound 1), apigenin (2), diosmetin (3), 5,7-dihydroxy-3′,4′,5′-trimethoxyflavone (4), acacetin (5), eupatilin (6), jaceosidin (7), and 6-methoxytricin (8). Luteolin is the most abundant compound in this fraction. All compounds significantly suppressed NO production in hepatocytes, with apigenin and acacetin showing the greatest efficacy. The comparison of the IC₅₀ values of the inhibition of NO production suggests that substitutions by hydroxyl and methoxy groups at the C-3′ and C-4′ positions of 5,7-dihydroxyflavone may be at least essential for the suppression of NO production. In hepatocytes, acacetin and luteolin decreased the levels of mRNAs encoding inducible nitric oxide synthase (iNOS), proinflammatory cytokines, including tumor necrosis factor, IL-6, and type 1 IL-1 receptor, which regulates inflammatory responses. Based on the comparison of the IC₅₀ values and the content, luteolin, jaceosidin, and diosmetin may be responsible for the anti-inflammatory effects of C. indicum capitula. Full article

Due to consumer needs and the prevalence of gluten-related disorders such as celiac disease, the gluten-free food market is expanding rapidly and is expected to surpass USD 2.4 billion by 2036. The objective of this study was to substitute wheat flour with oat, black rice, brown rice, buckwheat, and rice flours in the production of gluten-free scones, to assess consumer acceptability, and to identify factors contributing to consumer acceptability using check-all-that-apply questions. The 10 attributes of appearance, color, texture, grainy flavor, sweetness, familiar flavor, novelty, familiarity, moistness, and consistency exhibited statistically significant differences among the samples (p < 0.001). One hundred consumers evaluated 18 attributes using a nine-point hedonic scale, and all attributes demonstrated statistically significant differences across six samples (p < 0.001). The samples from buckwheat and wheat scored the highest in consumer acceptability. The results indicate a strong positive correlation between overall liking and purchase intention, with sensory attributes such as nutty flavor, cohesiveness, appearance, moistness, color, texture, and inner softness positively influencing consumer acceptability. The attributes affecting negatively were thick throat sensation, unique flavor, and stuffiness. This study is expected to provide data to aid in the development of better-tasting gluten-free products that meet customer and market needs. Full article

The escalating environmental and health concerns regarding conventional meat consumption have intensified the global search for sustainable dietary alternatives. Plant-based foods and meat substitutes have emerged as promising solutions. These products aim to replicate the sensory and nutritional attributes of meat while mitigating ecological impacts. This review examined the current scenario of plant-based foods and meat substitutes, focusing on their environmental footprints, health implications, innovative ingredient developments, consumer acceptance, and the use of analytical tools in quality control. Life cycle assessments indicate that plant-based foods and meat substitutes significantly reduce greenhouse gas emissions, land use, and water consumption compared to animal-based products. These alternatives offer benefits like lower saturated fat. However, they still struggle to match the amino acid composition of meat. Consumer acceptance is influenced by factors including taste, texture, and cultural perceptions, and still requires sensory improvement. Innovations in ingredient sourcing, like the use of legumes, mycoproteins, and fermentation-derived components, are enhancing product quality and diversity. Furthermore, analytical tools such as electronic noses, electronic tongues, spectroscopy, and chemometric methods ensure product consistency and fulfill consumer expectations. By synthesizing interdisciplinary insights, this review offers an integrated perspective to guide future research and development in the field of meat alternatives. Full article

Spinel-structured hausmannite (Mn(II)Mn(III)₂O₄) is a vital intermediate in Mn mineralogy and a key player in redox chemistry in the environment. Its transformation into other Mn oxides is a critical factor in controlling its environmental occurrence and reactivity. Yet structural impurities and solution pH, as well as the fate of impurities during transformation, which influence hausmannite transformation processes and products, remain largely unknown. In the present work, we address this knowledge gap by investigating pristine and metal-substituted hausmannite, specifically nickel (Ni) or cobalt (Co), equilibrated at two time periods (8 h and 30 days) and three different pH levels (4, 5, and 7). Solution chemistry data revealed that both the equilibration period and pH had a significant impact on hausmannite dissolution rates and the concomitant repartitioning of Ni or Co. Hausmannite with Ni or Co substitution exhibited lower dissolution rates than pristine mineral under acidic conditions. Mineralogy and crystal chemistry data indicated that hausmannite was the major host phase after 30-day equilibration, followed by minor transformed products, including birnessite and manganite. Although minor, birnessite became more abundant than manganite at low pHs. Analytical high-resolution transmission electron microscopy (HRTEM) analyses revealed a poorly crystalline, nano-scaled MnO₂ formed from hausmannite and the majority of metal impurities remaining in the host hausmannite. Yet Co was associated with both hausmannite and the newly formed birnessite, whereas Ni was only found with hausmannite, indicating the strong sequestration of Co by Mn(II/III) and Mn(IV) mineral phases. This study highlights the significant impacts of metal impurities and pH on the stability of hausmannite and its transformation into birnessite, as well as the control of Mn-oxide minerals on the solubility and sequestration of transition metals in the environment. Full article

In China, agriculture is currently highly dependent on chemical nitrogen. This leads to low nitrogen use efficiency and high nitrogen losses. Considering the issues caused by excessive chemical fertilizer, integrated nutrient management using organic and chemical fertilizer sources is important. To clarify how partial substitution of chemical fertilizer by organic fertilizer affects rice yield, physiological traits, and nitrogen use efficiency, we conducted a two-year field trial in 2021 and 2022, and used two rice cultivars, Shendao47 (SD47) and Shendao505 (SD505), which were grown in the field with five fertilization treatments: (1) CK (zero N application); (2) CF (100% chemical fertilizer); (3) OR10 (10% organic fertilizer + 90% chemical fertilizer); (4) OR20 (20% organic fertilizer + 80% chemical fertilizer); and (5) OR30 (30% organic fertilizer + 70% chemical fertilizer). The results show that the partial organic substitution (OR) treatments improved the yield by 1–10% for two cultivars by increasing effective panicles and grain filling. The increase in grain filling was related to the photosynthetic parameters, including LAI, chlorophyll content, and net photosynthetic rate during the grain-filling stage. The photosynthetic parameters of OR treatments were higher than those of CF treatment. Additionally, with the increase in organic fertilizer application rates, the grain yield, agronomic N use efficiency, partial factor productivity of applied N, and physiological N use efficiency increased at first and then decreased, peaking in OR20 treatment. Conclusively, the 20% organic fertilizer with 80% chemical fertilizer is a promising option for higher yield and improved N utilization for both cultivars. This study provides a sustainable nutrient management strategy to improve crop yield with high nutrient use efficiency. Full article

In this study, we explore the impact of land investment introduction on efficiency loss at both the enterprise and urban levels and discuss the role of factor-biased technological progress in minimizing these losses. Using a nested constant elasticity of substitution (CES) production function, we theoretically validate the premise that land investment introduction disrupts the optimal allocation of productive factors and reduces the “threshold selection” effect of land cost, leading to efficiency losses. Empirically, the systematic generalized method of moments (GMM) is applied to analyze panel data from 284 prefecture-level cities in China between 2007 and 2019. The findings reveal that land investment introduction brings about efficiency losses and prolonged land investment strategies that deepen enterprise efficiency loss, while urban efficiency loss may be temporarily alleviated but tends to deepen over the long term. Enterprise efficiency loss can be reduced by selecting land-biased, labor-biased, and capital-biased technological progress; however, its impact on urban efficiency loss remains uncertain. These findings provide insights into the optimal selection of factor-biased technological progress for industrial enterprises and provide policy-oriented recommendations for enhancing production and improving efficiency. Full article

Replacing chemical fertilizers with organic alternatives represents a viable strategy for enhancing agricultural productivity. The optimized integration of both fertilizer types can reduce the chemical input while improving soil conditions. However, the specific impacts of combined organic and inorganic fertilization on soil quality and crop performance require further investigation. To address this, a two-year field experiment was conducted to examine the effects of varying ratios of organic fertilizer substitution on wheat growth, grain yield, nutrient uptake, and soil quality. The results showed that the application of a 100% organic fertilizer combined with a 90% chemical fertilizer significantly increased the wheat biomass and grain yield. In terms of the nutrient uptake efficiency, the aboveground uptake of nitrogen (N), phosphorus (P), and potassium (K) increased significantly by 29.2%, 29.0%, and 56.5%, respectively. The nutrient use efficiency was also improved, with increases of 30.4% for N, 21.1% for P, and 47.7% for K. The partial factor productivity, total nutrient uptake, and the translocation efficiency of N, P, and K were all significantly enhanced. The soil quality was also markedly improved, with increases in both the soil organic matter and nutrient content. In conclusion, substituting chemical fertilizers with organic fertilizers improves the soil moisture and organic matter content, thereby enhancing the total uptake and translocation efficiency of nitrogen, phosphorus, and potassium. This leads to increased nutrient content in wheat grains, resulting in higher yields and improved grain quality. Moreover, this study provides practical guidance for wheat production and supports policy objectives related to sustainable agriculture, reduced chemical fertilizer use, and improved food security. Full article

This study investigates the performance of alkali-activated mortar incorporating slag, fly ash, and desert sand, with a focus on flowability, mechanical properties, sulfate resistance, and microstructural characteristics. A four-factor, three-level orthogonal experimental design was used to analyze the effects of the fly ash substitution rate, alkali content (Na₂O/b), activator modulus, and desert sand replacement rate for natural sand. The results indicate that increased slag and desert sand contents reduce mortar flowability. Despite this, the mortar exhibits excellent mechanical strength, with compressive strength reaching 77.7 MPa at 28 days and increasing to 89.34 MPa under sulfate exposure. However, after 120 days of sulfate erosion, a decline in strength is observed due to the formation of expansive products such as gypsum and caliche, leading to cracking. Microstructural analyses (XRD, SEM/EDS, MIP) reveal partial dissolution of desert sand under alkali activation, enhancing gel formation and reducing cumulative porosity. The pore structure predominantly consists of harmless pores. These findings demonstrate the potential of slag–fly ash–desert sand alkali-activated mortar as a durable and sustainable material for structural and construction engineering applications, especially in sulfate-rich environments or arid regions where desert sand is abundant. Full article

The intensive utilization of agricultural inputs is key to agricultural modernization. This study analyzed the elasticity of substitution among inputs in Chinese grain production (1991–2023) using a Translog production function, controlling for price disturbances. The key findings are as follows: (1) Complementary relationships exist between capital–fertilizer, capital–land, fertilizer–land, pesticide–land, and fertilizer–labor, while capital–pesticide, fertilizer–pesticide, pesticide–labor, and land–labor are substitutive. (2) The elasticity of substitution among agricultural inputs stabilizes over time, with substitutive and complementary relationships among most factors weakening after 2004. (3) Eastern and northeastern regions tend to substitute labor with capital more significantly, while central and western regions show a balanced interplay. (4) Nationwide trends in agricultural input shares indicate increasing mechanization, land-use efficiency, fertilizer use, and reduced labor input. These results provide insights for optimizing input allocation and enhancing food security. Full article

Secondary osteonecrosis (ON) is a common complication in paediatric cancer survivors. Combining multipotent mesenchymal stromal cells (MSCs) with core decompression surgery halts disease progression and stimulates bone regeneration. However, the success of advanced therapy medicinal products (ATMPs) requires versatile “off-the-shelf” tissue engineering products (TEPs). This study evaluated the safety and efficacy of TEPs loaded with allogeneic MSCs from Wharton’s jelly (WJ-MSCs) in a large-animal model of bone regeneration to support a paediatric investigational plan for ON patients. WJ-MSC-laden fibrin-based hydrogels combined with a synthetic bone substitute (PRO-DENSE^TM) were tested in 16 juvenile sheep (8 males and 8 females) distributed in four experimental groups. Each animal received four cylindrical bone defects in the femoral and tibial epiphyses and was assessed at 6 and 12 weeks. Safety was confirmed, and bone regeneration was observed across all groups. A combination of WJ-MSCs with PRO-DENSE^TM led to improved histological scores, osteogenesis, and construct integration. Trabecular bone volume also increased more in cellular groups over time. However, effects were inconsistent across groups, reflecting the variability seen in clinical trials and highlighting the significant impact of factors such as immunogenetic compatibility, MSC batch potency, and interaction with the recipient’s microenvironment on the therapeutic effectiveness and successful clinical translation of allogeneic ATMPs. Full article

Global agriculture remains dependent on nitrogen fertilizers produced through fossil fuel-based processes, contributing to greenhouse gas emissions, energy use, and supply chain vulnerabilities. This review introduces plasma-activated water (PAW) as a novel, electricity-driven alternative for sustainable nitrogen delivery. Generated by non-thermal plasma, PAW infuses water with reactive oxygen and nitrogen species, offering a clean, decentralized substitute for conventional synthetic fertilizers derived from the Haber–Bosch and Ostwald processes. It can be produced on-site using renewable energy, reducing transportation costs and depending on fertilizers. Beyond its fertilizer properties, PAW enhances seed germination, plant growth, stress tolerance, and pest resistance, making it a multifunctional input for controlled environment agriculture. We also assess PAW’s techno-economic viability, including energy requirements, production costs, and potential scalability through renewable energy. These factors are crucial for determining its feasibility in both industrial systems and localized agricultural applications. Finally, the review examines PAW’s contribution to the ten United Nations Sustainable Development Goals, particularly in climate action, clean energy, and sustainable food production. By combining agronomic performance with circular production and emissions reduction, PAW presents a promising path toward more resilient, low-impact, and self-sufficient agricultural systems. Full article

Balancing food security with fertilizer-driven climate impacts remains critical in intensive agriculture. While organic–inorganic substitution enhances soil fertility, its effects on nitrous oxide (N₂O) and nitric oxide (NO) emissions remain uncertain. This study evaluated N₂O/NO emissions, crop yields, and agronomic parameters in a subtropical wheat–maize rotation under four fertilization regimes: inorganic-only (NPK), manure-only (OM), and partial substitution with crop residues (CRNPK, 15%) or manure (OMNPK, 30%), all applied at 280 kg N ha⁻¹ yr⁻¹. Emissions aligned with the dual Arrhenius–Michaelis–Menten kinetics and revised “hole-in-the-pipe” model. Annual direct emission factors (EF_d) for N₂O and NO were 1.01% and 0.11%, respectively, with combined emissions (1.12%) exponentially correlated to soil nitrogen surplus (p < 0.01). CRNPK and OMNPK reduced annual N₂O+NO emissions by 15–154% and enhanced NUE by 10–45% compared with OM, though OMNPK emitted 1.7–2.0 times more N₂O/NO than CRNPK. Sole OM underperformed in yield, while partial substitution—particularly with crop residues—optimized productivity while minimizing environmental risks. By integrating emission modeling and agronomic performance, this study establishes CRNPK as a novel strategy for subtropical cereal systems, reconciling high yields with low greenhouse gas emissions. Full article