Search Results (31)

Vegetables such as lettuce, tomato, carrot, and beet are vital to the global food industry, providing essential nutrients and supporting sustainable agriculture. Their cultivation in greenhouses across diverse climatic zones (temperate, Mediterranean, tropical, subtropical, and arid) has gained prominence due to controlled environments that enhance yield and quality. However, these crops face significant threats from climate change, including rising temperatures, erratic light availability, and resource constraints, which challenge optimal growth and nutritional content. This study investigates the influence of microclimatic conditions—temperature, light intensity, and CO₂ concentration—on the growth, physiology, and biochemistry of these vegetables under varying greenhouse types and climatic zones, addressing these threats through a systematic review. The methodology followed the PRISMA guidelines, synthesizing peer-reviewed articles from 1995 to 2025 sourced from Web of Science, Pub Med, Scopus, Science Direct, Springer Link, and Google Scholar. Search terms included “greenhouse microclimate”, “greenhouse types”, “Climatic Zones, “and crop-specific keywords, with data extracted on microclimatic parameters and analyzed across growth stages and climatic zones. Eligibility criteria ensured focus on quantitative data from greenhouse studies, excluding pre-1995 or non-peer-reviewed sources. The results identified the following optimal conditions: lettuce and beet thrive at 15–22 °C, 200–250 μmol·m⁻²·s⁻¹, and 600–1100 ppm CO₂ in temperate zones; tomatoes at 18–25 °C, 200–300 μmol·m⁻²·s⁻¹, and 600–1100 ppm in Mediterranean and arid zones; and carrots at 15–20 °C, 150–250 μmol·m⁻²·s⁻¹, and 600–1000 ppm in subtropical zones. Greenhouse types (e.g., glasshouses, polytunnels) modulate these optima, with high-tech systems enhancing resilience. Conclusively, tailored microclimatic management, integrating AI-driven technologies and advanced greenhouse designs, is recommended to mitigate threats and optimize production across climatic zones. Full article

Drought severely impacts crop yields, especially wheat. Antitranspirants, which reduce water loss, have been shown to improve crop yield under drought, possibly by increasing pollen viability. To understand the mechanisms, transcriptomic responses were studied in early meiotic wheat anthers extracted from polytunnel-grown plants: well-watered, droughted unsprayed, and droughted plants treated with antitranspirants. Film (Vapor Gard (VG), di-1-p-menthene) and metabolic (Abscisic Acid (ABA), 20% S-ABA) antitranspirants were applied at the flag leaf stage (GS39). Well-watered (WW) plant anthers had more upregulated genes (626 genes) than downregulated ones (226 genes) when compared to droughted unsprayed ones. Most of the differentially expressed genes (DEGs) were transcriptionally downregulated (3959 genes) in droughted, treated-plant anthers (ABA and VG) compared with unsprayed (US), and the number of genes with upregulated expression was lower (830 genes). VG-treated plant anthers had more downregulated genes (3325 genes) than ABA-treated ones (634 genes). Carbohydrate or sugar metabolism and related processes were affected in antitranspirant-treated plant anthers with significant downregulation of genes compared to droughted unsprayed ones; in contrast, these processes were upregulated in well-watered anthers, suggesting broad differences in the transcriptional response. However, antitranspirants did not significantly affect pollen viability or yield in treated plants compared to unsprayed plants, suggesting that anthers are more sensitive at the transcriptomic level than subsequent physiological processes determining yield. Full article

This paper presents the results of laboratory tests on the intensity of mass and heat exchange in a polytunnel, with a focus on the synergy between the polytunnel and a stone accumulator. The subject of study was a standard polytunnel made of double polythene sheathing. In the process of selecting the appropriate working conditions for such a polytunnel, the characteristic operating parameters were modeled and verified. They were related to the process of mass and energy exchange, which takes place in regular controlled-environment agriculture (CEA). Then, experimental tests of a heat accumulator on a fixed stone bed were carried out. The experiments were carried out for various accumulator surfaces ranging from 18.7 m² to 74.8 m², which was measured perpendicularly to the heat medium. To standardize the results obtained, the analysis included the unit area of the accumulator and the unit time of the experiment. In this way, 835 heat and mass exchange events were analyzed, including 437 accumulator charging processes and 398 discharging processes from April to October, which is a standard period of polytunnel use in the Polish climate. During the tests, internal and external parameters of the process were recorded, such as temperature, relative humidity, solar radiation, wind speed and air flow speed in the accumulator system. Based on the parameters, a set of empirical relationships was developed using mathematical modeling. This provided the foundation for calculating heat gains as a result of its storage in a stone accumulator and its discharging process. The research results, including the developed dependencies, not only fill the scientific gap in the field of heat storage, but can also be used in engineering design of polytunnels supported by a heat storage system on a stone bed. In addition, the proposed methodology can be used in the study of other heat accumulators, not only in plant production facilities. Full article

Potatoes are highly sensitive to drought, particularly during tuber initiation. This study aimed to evaluate the effectiveness of film-forming (Vapor Gard [VG]) and metabolic (abscisic acid [ABA]) antitranspirants in mitigating drought stress and reducing tuber physiological disorders in four potato varieties. Two experiments examined the effects of VG and ABA antitranspirants on drought-stressed potato plants of four varieties (Challenger, Markies, Nectar, and Russet Burbank) grown in pots in a polytunnel (semi-controlled environment). Experiment 1 imposed severe drought by withholding irrigation until 70% of the available water content was depleted (reaching 15–17% volumetric water content within ~15 days), while Experiment 2 featured gradual drought stress from tuber initiation, with the soil volumetric water content declining to <10% over 30 days. Antitranspirants were applied at the start of the tuber initiation and two weeks later to assess their impact on the soil volumetric water content, stomatal conductance, relative water content, yield, and tuber physiological disorders. Drought significantly reduced the soil and plant water status, tuber yield, and quality across both experiments, with more severe effects observed in Experiment 1. VG and ABA had repeatable effects in both experiments and in all varieties, reducing water stress by preventing a large reduction in the relative water content during the tuber initiation and bulking stages. Both antitranspirants improved the tuber appearance by reducing the tuber skin disorder of russeting in the susceptible Challenger variety in both experiments, with VG being more effective than ABA. Beneficial reductions in the effects of drought from antitranspirants were also recorded in the volumetric water content, stomatal conductance, yield, and jelly end rot but not consistently in all varieties and in both experiments. The results show that antitranspirants have the potential to minimise water stress in droughted potatoes and subsequently reduce the physiological disorder of russeting and improve the tuber appearance of the Challenger variety. Full article

The food industry is increasingly turning to healthy and eco-friendly alternatives for meat preservation, with recent attention focused on chitosan (CH) and essential oils (EOs). Here, we propose two liquid formulations of CH enriched with Laurus nobilis or Piper nigrum EOs to preserve beef patties stored for 4 days at 4 °C from colour changes, secondary lipid oxidation, and alteration in volatile organic compound emissions while also preventing oviposition by Calliphora vomitoria on beef loaves hung for the same time at around 13 °C in a netted polytunnel. Overall, the L. nobilis EO-enriched CH solution increased the meat colour lightness compared to the control (+7.58%), kept redness and yellowness comparable to the control, maintained the level of thiobarbituric acid-reacting substances below the threshold for rancidity perception for at least 96 h, reduced the release of ethanol, enhanced the perception of fatty and woody notes in the meat along with the fresh, green, and citrusy aromas specific to the EO, and also provided significant protection (88.83%) against blowfly oviposition compared to the control. Therefore, the development of a spray CH formulation containing the L. nobilis EO appears to be a promising tool for stable and prolonged meat protection. Full article

Sustainable horticulture is crucially based on the greenhouse production of vegetables under controlled conditions. In this study, we wanted to learn how cultivated plants may impact indoor air quality and whether the workers can be exposed to bioaerosols in a similar way in these settings. The study objective was to test the hypothesis that the microbial concentrations, distribution of bioaerosol particle sizes, and composition of the airborne microbiome are specific to greenhouses, polytunnels, and open-air sites. The air samples were collected to assess the concentration of total culturable bacteria (TCB), fungi, actinomycetes, and β-haemolytic bacteria and for the identification of bacterial and fungal strains. Higher concentrations of TCB and fungi were found in the greenhouse (log 3.71 and 3.49 cfu m⁻³, respectively) than in polytunnels (log 2.60–2.48 and 2.51–2.31 cfu m⁻³, respectively) during the vegetation of cucumbers. These airborne microbes were represented by a significant contribution of the respirable fraction with a distinct contribution of fine particles in size below 4.7 µm. Cultivation of cucumbers resulted in the higher emission of airborne microorganisms in contrast with growing herbs such as oregano and basil. In total, 35 different bacteria and 12 fungal species, including pathogenic or allergenic agents, were identified within the studied sites. The workers can be exposed to increased concentrations of TCB and fungi in the greenhouse during the plant vegetation. It might be recommended to properly manage greenhouses and polytunnels, dispose of dust sources, and maintain appropriate ventilation to sustain relevant air quality. Full article

Cold conditions in northern China during winter may reduce sheep growth and affect their health, especially if they are young, unless housing is provided. We allocated 45 two-month-old female lambs to be housed in an enclosed building, a polytunnel, or kept outdoors, for 28 days. The daily weight gain and scalp and ear skin temperature of outdoor lambs were less than those of lambs that were housed in either a house or polytunnel; however, rectal temperature was unaffected by treatment. There was a progressive change in blood composition over time, and by the end of the experiment, outdoor lambs had reduced total antioxidant capacity (T-AOC), catalase (CAT), glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) and increased malondialdehyde compared to those in the house or polytunnel. In relation to immune responses in the lambs’ serum, in the polytunnel, immunoglobulin A (IgA), tumor necrosis factor-α (TNF-α) and interleukin-4 (IL-4) were higher and immunoglobulin G (IgG) lower compared with the concentrations in lambs that were outdoors. Over the course of the experiment, genes expressing heat shock proteins and antioxidant enzymes increased in lambs in the outdoor treatment, whereas they decreased in lambs in the indoor treatments. It is concluded that although there were no treatment effects on core body temperature, the trends for progressive changes in blood composition and gene expression indicate that the outdoor lambs were not physiologically stable; hence, they should not be kept outdoors in these environmental conditions for long periods. Full article

The objective of this research was to determine the effect of cassava (Manihot esculenta Crantz) supplementation on enteric methane (CH₄) emissions, carbon footprint, and production parameters in dairy cows. Daily concentrate supply for Jersey and Jersey * Holstein breeds was evaluated in four treatments (T): T1: 100% commercial concentrate; T2: 70% concentrate + 30% cassava leaves; T3: 70% concentrate + 30% cassava roots; and T4: 70% concentrate + 15% cassava leaves + 15% cassava root chips. Measurements of CH₄ emissions were performed using the polytunnel technique. Average daily dry matter intake ranged from 7.8 to 8.5 kg dry matter (DM). Cassava leaves were characterized by a high crude protein (CP) content (171 g CP/kg DM), with 5 times more neutral detergent fiber (NDF) content than cassava root (587 vs. 108 g NDF/kg DM). Average enteric CH₄ emissions per animal ranged from 194 to 234 g/d (p > 0.05). The carbon footprint was reduced by replacing 30% of the concentrate with cassava leaves and/or roots. Energy-corrected milk production was 1.15 times higher in Jersey * Holstein animals than Jersey cows (47 vs. 55 kg). Therefore, supplementation with cassava leaves and/or roots is a nutritionally and environmentally sustainable strategy. Full article

As the global population rises, there is an ever-increasing demand for food, in terms of volume, quality and sustainable production. Protected Cropping Structures (PCS) provide controlled farming environments that support the optimum use of crop inputs for plant growth, faster production cycles, multiple growing seasons per annum and increased yield, while offering greater control of pests, disease and adverse weather. Globally, there has been a rapid increase in the adoption of PCS. However, there remains a concerning knowledge gap in the availability of accurate and up-to-date spatial information that defines the extent (location and area) of PCS. This data is fundamental for providing metrics that inform decision making around forward selling, labour, processing and infrastructure requirements, traceability, biosecurity and natural disaster preparedness and response. This project addresses this need, by developing a national map of PCS for Australia using remotely sensed imagery and deep learning analytics, ancillary data, field validation and industry engagement. The resulting map presents the location and extent of all commercial glasshouses, polyhouses, polytunnels, shadehouses and permanent nets with an area of >0.2 ha. The outcomes of the project revealed deep learning techniques can accurately map PCS with models achieving F-Scores > 0.9 and accelerate the mapping where suitable imagery is available. Location-based tools supported by web mapping applications were critical for the validation of PCS locations and for building industry awareness and engagement. The final national PCS map is publicly available through an online dashboard which summarises the area of PCS structures at a range of scales including state/territory, local government area and individual structure. The outcomes of this project have set a global standard on how this level of mapping can be achieved through a collaborative, multifaceted approach. Full article

Carnarvon is a key horticultural district in Western Australia which is located approximately 900 km north of Perth and is characterised by a semi-arid climate. In Carnarvon, capsicum (Capsicum annuum L.) is the second most important vegetable crop after tomato, with approximately 3700 tonnes of capsicum fruit produced annually with a farm gate value of AUD 13.5 million. High temperatures, excessive sunlight, low air humidity, and strong wind in spring and summer are major impediments to the achievement of high yield and quality of capsicum in this region. Capsicums are usually planted between March (early autumn) and May (late autumn), and the harvest is usually finished by October (spring) of the same year when grown under shade net houses. However, the internal microenvironment in the shade net houses is sub-optimal for the crop in the early and late growing season due to excessive temperatures and low humidity, resulting in a shorter harvest window and lower production. This study was conducted to examine the possibilities to extend the cropping season for capsicum varieties (i.e., Chevello and Chevi) grown under the retractable roof production system (RRPS) and explore an alternative protected cropping structure that is more affordable and suitable to grow vegetable crops under Carnarvon weather conditions. Overall, the results showed that capsicums planted in February (planting 1) performed better than specimens planted later on in the season: planting 1 performed better and yielded the highest marketable fruit yield (102.6 t ha⁻¹) compared to those planted in early April (planting 2, 72.5 t ha⁻¹) and late May (planting 3, 36.1 t ha⁻¹). The RRPS effectively mitigated the adverse weather conditions and provided a more optimised internal microenvironment for vigorous crop establishment in late summer and an extended harvest in late spring, leading to a higher marketable fruit yield per crop. The total soluble solids were cultivar-specific, with the Brix level of Chevello changing with planting time while those of Chevi remained constant. The study identifies the potential for an alternative protected cropping structure, i.e., the modified multi-span polytunnels. The technical feasibility and affordability of the alternative protected cropping structure is also discussed. Full article

The use of plastics in horticulture is reviewed with respect to its sustainability based on the traditional criteria of triple Rs (reduce, reuse, and recycling) plus a re-place strategy, taking into account possible alternatives. Hail (and insect) nets made of HD-PE, with their long-term use mostly on apple and polytunnels of LD-PE for cherry and strawberry as well as solarisation mulches (reuse), were found to be relatively sustainable solutions for their needs and are currently without alternatives. In contrast, standard black mulch, with its largest share among horticultural plastics, had the widest range of sustainable alternatives, ranging from biodegradable to spray mulch; few sustainable alternatives are available for fleeces and reflective mulches. For the third sustainable option, pilot recycling schemes were examined, such as PolieCo^TM (Italy), MAPLA^TM (Spain), and ERDE^TM (Germany); they collect 30–50% of the agricultural plastics used in their respective areas, with a successful retrieval growth rate of ca. 20% per year in the case of ERDE^TM. For the fourth new R option (replace), future sustainability perspectives for the predominant black mulch are research into and development of better, biodegradable, non-fossilbased plastics, sprayable mulch; microbes for the digestion of deployed polyolefins and, for a certain limited range (on shade tolerant crops or in high-light intensity environment), hail nets and polytunnels that are equipped/substituted by/with solar panels (“agri pv”) for the concomitant sustainable production of green renewable energy. Full article

Blueberry, hailed as an antioxidant superfood, is the fruit of small shrubs in the genus Vaccinium (family Ericaceae). The fruits are a rich source of vitamins, minerals and antioxidants such as flavonoids and phenolic acids. The antioxidative and anti-inflammatory activities derived from the polyphenolic compounds, particularly from the abundantly present anthocyanin pigment, have been highlighted as the major contributing factor to the health-benefitting properties of blueberry. In recent years, blueberry cultivation under polytunnels has expanded, with plastic covers designed to offer protection of crop and fruit yield from suboptimal environmental conditions and birds. An important consideration is that the covers reduce photosynthetically active radiation (PAR) and filter out ultraviolet (UV) radiation that is critical for the fruit’s bioactive composition. Blueberry fruits grown under covers have been reported to have reduced antioxidant capacity as compared to fruits from open fields. In addition to light, abiotic stresses such as salinity, water deficit, and low temperature trigger accumulation of antioxidants. We highlight in this review how interventions such as light-emitting diodes (LEDs), photo-selective films, and exposure of plants to mild stresses, alongside developing new varieties with desired traits, could be used to optimise the nutritional quality, particularly the content of polyphenols, of blueberry grown under covers. Full article

Simulation models represent a low-cost approach to evaluating agricultural systems. In the current study, the precision and accuracy of the RUMINANT model to predict dry matter intake (DMI) and methane emissions from beef cattle fed tropical diets (characteristic of Colombia) was assessed. Feed intake (DMI) and methane emissions were measured in Brahman steers housed in polytunnels and fed six forage diets. In addition, DMI and methane emissions were predicted by the RUMINANT model. The model’s predictive capability was measured on the basis of precision: coefficients of variation (CV%) and determination (R², percentage of variance accounted for by the model), and model efficiency (ME) and accuracy: the simulated/observed ratio (S/O ratio) and slope and mean bias (MB%). In addition, combined measurements of accuracy and precision were carried out by means of mean square prediction error (MSPE) and correlation correspondence coefficient (CCC) and their components. The predictive capability of the RUMINANT model to simulate DMI resulted as valuable for mean S/O ratio (1.07), MB% (2.23%), CV% (17%), R² (0.886), ME (0.809), CCC (0.869). However, for methane emission simulations, the model substantially underestimated methane emissions (mean S/O ratio = 0.697, MB% = −30.5%), and ME and CCC were −0.431 and 0.485, respectively. In addition, a subset of data corresponding to diets with Leucaena was not observed to have a linear relationship between the observed and simulated values. It is suggested that this may be related to anti-methanogenic factors characteristic of Leucaena, which were not accounted for by the model. This study contributes to improving national inventories of greenhouse gases from the livestock of tropical countries. Full article

Cultivation of strawberries in greenhouses and polytunnels is increasing, and new sustainable growing media are needed to replace peat and coconut coir. This study investigated the effect of wood fiber and compost as growing media on hydroponically cultivated strawberries. Two experiments were conducted, where the everbearing cultivar ‘Murano’ was grown in mixtures of wood fiber and compost (Experiment 1) and the seasonal flowering cultivar ‘Malling Centenary’ was grown in mixtures of wood fiber and peat (Experiment 2). Additionally, in Experiment 2, the effect of adding start fertilizer was assessed. The yield potential of ‘Murano’ plants was maintained in all substrates compared to the coconut coir control. However, a mixture of 75% wood fiber and 25% compost produced the highest yield, suggesting that mixtures of nutritious materials with wood fiber may improve plant performance. The chemical composition of the berries was not affected by the substrate composition; however, berries from plants grown in the best performing blend had a lower firmness than those grown in coconut coir. ‘Malling Centenary’ plants produced higher yields in substrates enriched with start fertilizer. Generally, the productivity of ‘Malling Centenary’ plants was maintained in blends containing up to 75% of wood fiber mixture even without start fertilizer. Full article

Peppermint (Mentha x piperita) is a species with inhibitory allelopathic properties due to its high amounts of terpenes. Recent studies have disclosed dosage dependent growth promotion or defense reactions in plants when facing appropriate amounts of Mentha bouquet terpenes. These positive effects could be of interest for agricultural applications. To obtain more insights into leaf growth modulations, the expression of Arabidopsis and Brassica rapa TCP transcription factors were studied after fumigation with M. x piperita bouquets (Arabidopsis), with M. x piperita essential oil or with limonene (Arabidopsis and Chinese cabbage). According to qPCR studies, expression of TCP3, TCP24, and TCP20 were downregulated by all treatments in Arabidopsis, leading to altered leaf growth. Expressions of B. rapa TCPs after fumigation with the essential oil or limonene were less affected. Extensive greenhouse and polytunnel trials with white cabbage and Mentha plants showed that the developmental stage of the leaves, the dosage, and the fumigation time are of crucial importance for changed fresh and dry weights. Although further research is needed, the study may contribute to a more intensive utilization of ecologically friendly and species diversity conservation and positive allelopathic interactions in future agricultural systems. Full article