Search Results (28,881)

Background/Objectives: Milk somatic cells reflect the cellular composition and functional state of the lactating mammary gland and represent a valuable, non-invasive source for transcriptomic studies. Single-cell RNA sequencing (scRNA-seq) enables cell-type-resolved analysis of bovine milk; however, sequencing depth strongly influences the detection of lowly expressed genes and the resolution of transcriptional cell states. The aim of this study was to further characterise the single-cell transcriptome of bovine milk somatic cells, with particular emphasis on high-resolution gene expression profiling and cellular heterogeneity. Methods: Milk somatic cells were isolated from two healthy Holstein Friesian cows in mid-lactation and profiled using a droplet-based scRNA-seq platform. Newly generated high-depth datasets were integrated with two previously published bovine milk scRNA-seq datasets using an identical bioinformatics pipeline. Data integration, clustering and cell-type annotation were performed using the Seurat framework, and transcription factor expression was evaluated across datasets with different sequencing depths. Results: Single-cell transcriptomic analysis revealed a diverse cellular landscape in bovine milk, comprising epithelial, progenitor, and immune cell populations. Unsupervised clustering identified 21 transcriptionally distinct clusters, including multiple CD8⁺ T-cell subpopulations, monocytes, neutrophils, mast cells, and B cells, as well as luminal epithelial and luminal progenitor cells. While overall cell-type composition was comparable across datasets, deeply sequenced samples exhibited higher transcriptomic complexity and enabled refined resolution of immune and epithelial subpopulations. Deeper sequencing facilitated the detection of low-abundance transcription factors that were not observed in lower-depth datasets. Among these, NANOG was detected exclusively in deeply sequenced samples, suggesting the presence of rare transcriptional states associated with cellular plasticity. Conclusions: This study expands the single-cell transcriptomic landscape of bovine milk somatic cells and demonstrates the importance of sequencing depth for resolving functional cellular heterogeneity. The results highlight milk as a powerful, non-invasive source for investigating mammary gland biology and cellular plasticity during lactation. Full article

This study systematically investigated the bearing capacity and failure mechanisms of ultra-high-performance concrete (UHPC) pipe jacking structures using three-edge bearing tests and numerical simulations. Full-scale double-layer reinforced pipes had an inner diameter of 2.5 m and wall thicknesses of 180 mm (P1) and 200 mm (P2). The tests showed that the failure process can be divided into four stages: elastic deformation, crack propagation, reinforcement yielding, and ultimate failure. Increasing the wall thickness significantly improved performance: P2 had a cracking load 52.73% higher and an ultimate bearing capacity 5.7% higher than P1, with better deformation resistance and crack control. A theoretical model considering the plastic hinge mechanism at the pipe crown was developed, treating the three-edge load as an equivalent distributed plate load. The calculated results agreed well with experimental measurements. An ABAQUS finite element model successfully reproduced the full mechanical response from initial loading to failure. Parametric analysis indicated optimal performance at a hoop reinforcement ratio of approximately 1.4%. Even at 0.6%, the ultimate bearing capacity reached 367 kN/m, meeting current design code requirements. This study is novel in conducting full-scale UHPC pipe jacking tests, proposing a theoretical model accounting for crown plastic hinges, and establishing a finite element method that reproduces the entire failure process. Optimizing wall thickness and hoop reinforcement can enhance structural safety and durability, providing guidance for the design and engineering of pipe jacking structures. Full article

Background: Sensorimotor differences have frequently been reported in children with developmental dyslexia, but are often considered secondary or comorbid to phonological deficits. Within an embodied cognition perspective, reading acquisition emerges from dynamic interactions between bodily regulation, multisensory integration, and learning-related neural plasticity. Proprioception contributes to spatial orientation, motor coordination, and perceptual stabilization, while sleep-dependent processes play a critical role in the consolidation and automatization of cognitive and motor skills. Objectives: Building on early clinical observations, including the hypothesis proposed by Martins da Cunha, this review explores whether variations in proprioceptive processing and sensorimotor regulation may influence multisensory stability and the conditions under which reading skills develop in some individuals with dyslexia. Methods: This narrative synthesis integrates clinical observations and experimental paradigms examining proprioceptive function in children with dyslexia, including studies conducted in our laboratory over the past two decades. These investigations address postural regulation under varying attentional demands, laboratory measures of proprioceptive acuity, visuospatial localization tasks, multisensory interactions, and exploratory observations concerning sleep–wake regulation. Results: Across studies, children with dyslexia often show differences in proprioceptive processing associated with variations in postural regulation, visuospatial stability, and multisensory tasks. Laboratory measurements suggest reduced proprioceptive acuity in some individuals, with moderate correlations observed between proprioceptive sensitivity and reading-related measures. Additional observations suggest that nocturnal physiological regulation—including respiratory dynamics and sleep architecture—may interact with daytime sensorimotor stability and attentional functioning. Conclusions: Taken together, these findings support the hypothesis that variations in sensorimotor regulation across the sleep–wake cycle may influence the stability of multisensory processing and attentional conditions relevant for reading acquisition. Within this perspective, proprioception is not proposed as an alternative explanation for dyslexia but as a complementary dimension that may contribute to the heterogeneity of dyslexic profiles. Further longitudinal and controlled studies are required to clarify the relationships between sensorimotor regulation, sleep-dependent plasticity, and learning processes. Full article

This study explores the use of spectral methods for fatigue life assessment, considering the effects of material plasticity. While these methods are widely used for high-cycle fatigue in the linear elastic regime, their application to low-cycle fatigue remains more complex due to nonlinear material behaviour. By incorporating models such as Neuber’s rule and the Ramberg-Osgood formulation, this work examines how spectral methods can be adapted to account for elastic-plastic effects. A comparison is made between fatigue life estimations obtained with spectral approaches and results from time-domain nonlinear simulations. The study provides insights into the applicability of strain-based spectral methods, contributing to a better understanding of their potential and limitations in fatigue assessment. Full article

Plastic waste gasification offers a dual-benefit pathway for hydrogen production and waste management in emerging economies. However, existing hydrogen infrastructure planning focuses predominantly on blue and green pathways, with limited integration of waste-derived hydrogen or spatially distributed waste availability constraints. This study determines optimal waste-to-hydrogen infrastructure deployment in Oman through 2040 using mixed-integer linear programming with verified techno-economic parameters. Results indicate that plastic waste can produce 21,997 tonnes H₂ annually at a levelised cost of $2.88/kg, competitive with blue hydrogen ($1.80–2.50/kg) and significantly cheaper than current green hydrogen ($4–6/kg). The optimal network comprises four facilities at Muscat (500 TPD), Sohar (128 TPD), Salalah (192 TPD), and Nizwa (67 TPD), processing 275,000 tonnes of plastic waste whilst avoiding 137,000 tonnes of CO₂-eq through landfill diversion. However, feedstock availability constrains production to 24% of base case demand (90,000 tonnes), positioning waste-to-H₂ as a complementary pathway requiring integration with steam methane reforming for industrial hubs and electrolysis for the transport sector. Sensitivity analysis reveals hydrogen yield (±29% cost impact) and CAPEX (±20%) as critical parameters, with cost reduction pathways targeting $2.00–2.30/kg by 2035 through technology learning and co-benefit monetisation. Policy recommendations include extended producer responsibility schemes, government fleet procurement mandates, and regional waste trade agreements across the GCC. Waste-to-hydrogen demonstrates techno-economic viability as a guaranteed baseload contributor within diversified hydrogen strategies for Gulf economies. Full article

Separate collection and treatment systems for municipal solid waste (MSW) are designed to support efforts in sustainability. Biowaste accounts for the majority of MSW; thus, its proper management is essential. This study analyzes the impact of the presence of composting or anaerobic digestion (AD) facilities on MSW management. The management systems in Poland with composting and AD facilities were compared. Five fractions, including mixed/residual waste and biowaste, were collected in the analyzed regions; however, the rules for sorting biowaste varied. A drop in the collected residual/mixed MSW was noticed (by 3.8% to 6.6% year-on-year), while the biowaste stream increased, resulting in a 4–10% increase in the share of biowaste. The proportion of the organic fraction in biowaste was found to be 85–88%, but the proportion of food waste was slightly higher in the region with an AD facility (by about 3%). Plastics were the primary contaminants, accounting for approximately 5.5%. The presence of AD facilities has a positive impact on MSW management, including higher biowaste collection levels (67.5 kg per person versus 48.1 kg per person). Furthermore, under comparable regional conditions, economic gains were observed, such as relatively lower gate fees for biowaste (about 57 EUR per ton versus about 62 EUR per ton) and greater differences in fees between biowaste and residual/mixed MSW (about 80 EUR per ton versus about 14 EUR per ton). Full article

Pharmacological antidepressant treatments alter the molecular and functional reactivity of stress-sensitive neural networks. However, how classical versus rapid-acting antidepressants differentially modulate acute stress-induced transcriptional responses across brain regions remains unclear. Here, we compared imipramine and ketamine in mice exposed to acute swim stress, assessing transcriptional adaptations across the frontal cortex, hippocampus, and striatum. Swim stress induced significant widespread activation of cFOS, which led to drug-specific modulations: imipramine primarily significantly dampened cortical and striatal cFOS expression, whereas ketamine preserved stress-evoked neuronal activation. In contrast, hippocampal activation was significantly robust but largely unaffected, indicating that acute antidepressant drug effects during stress coping preferentially target cortical and striatal plasticity mechanisms. In contrast, BDNF expression was altered only within the striatal region, where imipramine attenuated the stress-related increase in BDNF expression. Statistical analysis of behavioral outcomes during the swim stress confirmed a shared facilitation of active coping, yet these similar outcomes emerged from distinct molecular programs. Together, the data demonstrate that the treatment effects of the two substances diverge mechanistically, revealing cortical and striatal transcriptional signatures of classical versus rapid-acting antidepressant action. While these findings suggest potential translational relevance for understanding distinct mechanisms, further studies in humans are required to validate these signatures and their clinical implications. Full article

Plastic film mulching (PFM) is widely used in arid, semiarid, and seasonally arid regions, where it plays a key role in regulating agricultural productivity and CO₂ emissions. Our study aims to clarify the effects of PFM on crop yield, CO₂ emissions, and the associated tradeoffs, providing a theoretical basis for the sustainable use of PFM in agriculture. We conducted a meta-analysis to compare differences in crop yield, CO₂ emissions, and yield-scaled CO₂ emissions (YSC) between mulching and no mulching treatments while identifying factors influencing these outcomes. Our findings demonstrated that PFM enhanced crop yields of maize, wheat, and cotton by 33.2% (p < 0.001), 21.8% (p < 0.05), and 26.3% (p < 0.05), respectively. PFM stimulated CO₂ emissions in maize fields by 36.8% (p < 0.001), while decreasing them in wheat and cotton fields by 11.8% (p < 0.05) and 8.1% (p > 0.05), respectively. Consequently, PFM significantly lowered YSC for maize by 39.3% (p < 0.05) and reduced it for cotton by 27.4% (p > 0.05), but led to a 38.3% increase in YSC for wheat (p > 0.05). For maize and cotton, when crop yields exceeded 6 t/ha, the YSC under plastic film mulching was higher than that under non-mulching. In contrast, for wheat, within the conventional yield range (below 10 t/ha), the YSC under plastic film mulching was lower than that under non-mulching. For cotton, the lowest YSC under PFM was achieved under the combined conditions of water inputs > 500 mm, air temperature > 8 °C, soil pH > 8, and N inputs < 200 kg N ha⁻¹. For wheat, the lowest YSC under PFM was obtained under water inputs < 350 mm, air temperature < 8 °C, light-texture soils, and N inputs < 200 kg N ha⁻¹. For maize, the lowest YSC under PFM was achieved under water inputs < 350 mm, air temperature < 8 °C, heavy-texture soils, soil pH < 8, and N inputs < 200 kg N ha⁻¹. These insights offer guidance for the optimal use of PFM to enhance carbon efficiency and crop yield in agricultural systems. Full article

Infertility is a prevalent clinical issue which disrupts normal human life and exerts an impact on fertility rates within the population. The increase in environmental pollutants, including acetyl tributyl citrate (ATBC), has given rise to concerns regarding their potential toxicity in infertility-related disorders. Icariin exhibits therapeutic effects on infertility, yet its mechanism of action against plasticiser-induced reproductive disorders remains unclear. This study aims to elucidate the potential toxicological targets and molecular mechanisms of ATBC-induced infertility, as well as the therapeutic targets and mechanisms of icariin in treating ATBC-induced reproductive disorders, through network toxicology, molecular-docking techniques and molecular dynamics simulation. Utilising the component-target database SwissTargetPrediction, the Similarity Ensemble Approach, PharmMapper, the ChEMBL database, and disease databases including the Therapeutic Target Database, OMIM, GeneCards, and DrugBank, 63 targets for ATBC-induced infertility and 33 targets for icariin treatment were identified. Screening via the STRING platform and Cytoscape 3.10.1 software yielded four core targets for ATBC-induced infertility—HSP90AA1, PIK3CA, CASP3, HRAS—and four core targets for icariin treatment—IL6, TNF, STAT3, and INS. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that ATBC-induced infertility correlates with pathways including pathways in cancer, prostate cancer, and PI3K-Akt signalling pathways. Conversely, the core targets of icariin therapy for related reproductive disorders are closely associated with tumour-associated signalling pathways and the AGE-RAGE signalling pathway. Molecular-docking and molecular dynamics simulation further confirmed the strong binding interactions between ATBC and infertility-related targets, as well as between icariin and core targets for treating reproductive disorders. This provides a theoretical foundation for understanding ATBC’s toxicological targets and the complex molecular mechanisms underpinning icariin’s treatment of infertility. It informs the development of strategies for icariin to prevent and treat infertility caused by exposure to ATBC-containing plastics or excessive ATBC contact. Full article

The etiology of autism spectrum disorder (ASD) implicates genetic predispositions and environmental chemicals, such as polybrominated diphenyl ethers (PBDEs). We aimed to identify whether mitochondrial quality control (MQC) was involved in ASD-relevant behavioral changes induced by decabromodiphenyl ether (deca-BDE, BDE-209) and the alleviation by melatonin. Pregnant rats exposed to BDE-209 (50 mg/kg i.g.) were administrated melatonin through drinking water (0.2 mg/mL) during gestation and lactation. Behavioral assessments integrated open-field test, three-chamber social test, and Morris water maze; mitochondrial detections took transmission electron microscopy, immunofluorescence, and homeostasis together; hippocampal molecular network was identified through transcriptomics profiles, combining dendritic morphology analysis after Golgi-Cox staining. Melatonin supplementation attenuated BDE-209-reduced social and cognitive ability, accompanied by improvements in hippocampal synaptic plasticity (dendritic spines, PSD95, SNAP25). Mitochondrial dysfunctions, shown as decreases in complex IV activity, ATP content, and mtDNA copies, plus redox imbalance (ROS/SOD2) and resultant mitochondrial membrane potential disruption and apoptosis, together with fusion/fission dynamic (MFN2/DRP1), biogenesis (SIRT1-PGC1α-TFAM), and mitophagy (SIRT3-FOXO3-PINK1) suppression, were reversed by melatonin partially through SIRT1 (Sirtuin-1)-dependent pathways, as these protections were abolished by inhibitor EX527. This study highlighted the SIRT1–SIRT3 axis in MQC and behavioral effects, providing novel intervention for PBDEs’ neurodevelopmental impairment. Full article

This study investigates the effects of the co-curing process and nanoparticle reinforcement on the mechanical performance of plain-woven glass fiber-reinforced plastic (GFRP) adhesive joints, aiming to address the limitations of traditional fastening methods and the inherent brittleness of epoxy adhesives. Specifically, spherical silica (SiO₂) and plate-like graphene nanoplatelets (GNPs) were incorporated into the epoxy matrix at varying concentrations (0.25 to 1.0 wt.%) to evaluate the influence of particle geometry on joint integrity. Experimental results demonstrated that the co-curing technique yields superior mechanical properties compared to secondary bonding, exhibiting improvements of 35% in shear strength (from 10.97 MPa to 14.83 MPa) and 12% in flexural strength (from 72.57 MPa to 81.28 MPa) due to enhanced chemical interlocking. Furthermore, the addition of nanoparticles significantly improved joint performance, with the optimal content identified at 0.75 wt.% for both particle types. Notably, GNPs outperformed SiO₂, enhancing shear and flexural strengths compared to the neat co-cured baseline. Ultimately, the 0.75 wt.% GNP-reinforced material exhibited a shear strength of 21.22 MPa and a flexural strength of 104.09 MPa. Morphological analysis revealed that while SiO₂ contributes to reinforcement primarily via crack deflection, the high-aspect-ratio GNPs provide superior energy dissipation through crack bridging and pull-out mechanisms. Consequently, this study suggests that the co-curing process combined with an optimal concentration of GNPs presents a highly effective strategy for maximizing the reliability and structural efficiency of composite joints in weight-critical applications. Full article

Spinal cord injury (SCI) induces persistent locomotor deficits that are closely associated with maladaptive structural plasticity of spinal neuronal circuits. Although motor rehabilitation improves functional outcomes, the cellular substrates underlying rehabilitation-induced recovery remain incompletely understood, particularly in relation to activity-dependent neuromodulation strategies. Here, we investigated how treadmill-based motor training (TMT) and its combination with bioluminescent optogenetic (BL-OG) stimulation of Hb9 (homebox 9)-positive motoneurons and excitatory interneurons selectively modulate microarchitectural plasticity in the injured rat spinal cord. At the level of gross locomotor assessment, Basso, Beattie and Bresnahan (BBB) scores were comparable between the BL-OG and SCI+TMT groups. Although no statistically significant differences in the total score in rung ladder were observed at 28 days post-injury, animals in the BL-OG group showed a tendency toward a higher ratio of successful hindlimb placements, indicating improved step accuracy. BL-OG stimulation was associated with a slightly greater attenuation of SCI-induced spine abnormalities compared to TMT alone, with significant differences between the experimental groups detected specifically in laminae VIII and IX. These lamina-specific alterations in dendritic integration and dendritic spine composition were accompanied by preservation of wisteria floribunda agglutinin WFA-positive perineuronal net (PNN) architecture. Against this background, reduced glypican-4 (GPC-4) expression and attenuated WFA/GPC-4 colocalization were observed in the SCI+BL-OG group relative to SCI in laminae VII–IX, consistent with activity-dependent modulation of PNN-associated synaptic organization in Hb9-positive neuronal populations. Together, these findings indicate that motor rehabilitation and bioluminescent optogenetic stimulation engage distinct but partially overlapping mechanisms of activity-dependent microarchitectural remodeling, preferentially targeting synaptic and perineuronal net-associated substrates rather than inducing large-scale circuit reorganization. Further studies are warranted to elucidate the mechanisms underlying these distinct plasticity profiles. Full article

Background/Objectives: Speech-evoked brain potentials provide a window into the neural encoding of speech, experience-dependent plasticity, and deficits in central auditory processing from communication disorders. Stronger and faster frequency-following responses (FFRs) and cortical event-related potentials (ERPs) have been interpreted as reflecting more robust and efficient auditory–sensory processing across brainstem and cortical levels. Importantly, these neural signatures relate to real-world listening skills like speech-in-noise (SIN) perception. How functional FFR/ERPs relate to the underlying anatomical structures that generate these responses in brainstem and cortex is unknown. Methods: Using a multimodal imaging approach, we recorded FFRs and ERPs to clean and noise-degraded speech sounds to assess the strength of listeners’ neural encoding of speech at brainstem (FFR) and cortical (ERP) levels. MRI volumetrics of midbrain and transverse temporal gyrus (Heschl’s gyrus) quantified morphological variation in subcortical and cortical anatomy that underly these EEG potentials. We used the QuickSIN to assess behavioral SIN abilities. Results: We found larger and thicker right (but not left) Heschl’s gyrus was related to listeners’ SIN perception as well as the size of their cortical ERPs. Structural and functional measures interacted at a subcortical level. For listeners with smaller midbrain volumes, larger speech FFRs were associated with better QuickSIN scores, whereas in individuals with larger midbrain volumes, larger FFRs were related to poorer QuickSIN scores. Conclusions: Our findings reveal common functional signatures of speech sound processing (FFRs, ERPs) are related to the anatomy of their underlying generator sources and suggest that both auditory brain structure and function can account for perceptual SIN capacity. Full article

As a representative digital additive construction material, three-dimensional printed concrete (3DPC) imposes a synergistic rheological requirement on fresh cementitious mixtures, namely “pumpability–extrudability–buildability,” throughout the forming process. Rheological parameters and their temporal evolution not only govern the stability of the material during pumping, nozzle extrusion, and layer-by-layer deposition, but also directly determine interlayer interfacial integrity, geometric fidelity, and the development of macroscopic mechanical performance. This paper provides a systematic review of the regulation strategies and evolutionary characteristics of 3DPC rheology, with particular emphasis on how raw material composition, printing parameters, and multiscale evolution mechanisms influence yield stress, plastic viscosity, and thixotropic behavior. The time-dependent evolution of rheological properties is elucidated across multiple length scales, encompassing microscopic particle interactions and hydration-induced bridging, mesoscopic aggregate force-chain networks and particle migration, and macroscopic shear stimulation coupled with temperature–humidity effects. On this basis, it is further highlighted that existing models and characterization frameworks remain insufficient to capture the time-dependent structural evolution under realistic printing conditions. Therefore, the establishment of unified characterization standards, together with in situ rheological measurements and multiscale simulations, is urgently required to enable the coordinated optimization of material design and printing processes and to facilitate engineering-scale implementation. Full article

To investigate the load characteristics and rock-breaking efficiency of the hobs on the conical cutterhead, a theoretical model of the hob’s rock-breaking load was established based on the plastic-brittle characteristics of rock, with a verification error of less than 5%. A numerical model of dual-hob rotary rock breaking was developed using ABAQUS 2022 software to comparatively study the influence of penetration depth (P), cutter spacing (S), and rotational speed (V) on the hob’s load behavior and rock-breaking efficiency. The specific energy of rock breaking under various test conditions was obtained through orthogonal experiments. The results indicate that, as the penetration depth increases, the average rock-breaking load of the hob gradually increases, while the specific energy first decreases and then increases. With larger cutter spacing, the average load shows a modest increase, and the specific energy exhibits a gradually rising trend with a diminishing growth rate. As the rotational speed increases, the average load increases slightly, while the specific energy rises with an accelerating growth rate. Range analysis revealed that the order of influence of factors on rock-breaking efficiency is P > S > V. The highest rock-breaking efficiency was achieved at P = 2 mm, S = 60 mm, and V = 7 r/min. At a significance level of 0.05, the penetration depth was found to have a significant effect on specific energy. This study provides a valuable reference for the design of hob layouts and parameter settings of conical cutterheads, contributing to improved rock-breaking efficiency. Full article