Search Results (31)

Fatty liver is a major metabolic disease in periparturient dairy goats. Protein ubiquitination, a type of dynamic and multifaceted post-translational modification, plays an important role in metabolism by regulating the stability and function of target proteins. However, the hepatic protein ubiquitination profile in dairy goats with fatty liver is yet to be elucidated. In this study, we collected liver and blood samples from healthy dairy goats (Con, n = 3) and dairy goats with fatty liver (FL, n = 3). Then, we analyzed the overall ubiquitination of hepatic proteins in dairy goats with fatty liver through quantitative ubiquitin label-free proteomics and bioinformatics. Proteins showing significantly altered levels of ubiquitination were identified via bioinformatics, and related regulatory pathways were screened. The results showed that the blood levels of beta-hydroxybutyric acid and non-esterified fatty acids were significantly upregulated in dairy goats with fatty liver, and a total of 238 ubiquitination sites across 921 proteins were found to be differentially altered in the fatty liver group. Among them, ubiquitination was upregulated at 351 sites across 93 proteins and downregulated at 570 sites across 145 proteins. In addition, GO and KEGG pathway analysis revealed that the differentially ubiquitinated proteins were enriched in pathways regulating lipid metabolism, such as the PPAR signaling pathway, fatty acid degradation, and peroxisome activity. Notably, by observing the overlap among these three sub-networks, we found that proteins with downregulated ubiquitination—such as ACSL1, ACSL5, EHHADH, and ACAA1—were transcriptionally upregulated in dairy goats with fatty liver. This study reveals the key ubiquitinated proteins in dairy goats with fatty liver and provides a more comprehensive understanding of the pathogenesis of fatty liver in dairy goats. Full article

Effective health management during the transition period depends on early disease detection, which can be achieved through continuous monitoring using precision livestock farming tools. This study assessed reticulorumen temperature, rumination time, and activity in dairy cows during the periparturient period under summer heat stress. We hypothesized differences in these parameters between healthy (HE) cows and those developing postpartum disorders (DI). Forty clinically healthy, multiparous cows were monitored from 5 days prepartum to 14 days after calving (days in milk; DIM). A cow was considered healthy and allocated to the HE group (n = 26) if she was not affected by any postpartum health disorders until the end of the study period. A cow was considered diseased and allocated to the DI group (n = 14) if she had been diagnosed with mastitis, metritis, lameness, or ketosis. Weather loggers recorded barn microclimate data, while rumination, activity, and rumen temperature were tracked using a microphone-based sensor in the neck collar (Ruminact HR) and rumen bolus (Smaxtec). THI values remained above 68 throughout the study, peaking at 80, indicating sustained heat stress. Rumen temperature ranged between 39 and 41 °C and moderately correlated with THI (correlation coefficient was 0.27; 95% CI: 0.20; 0.33; p < 0.0001). Both groups exhibited a nadir in rumen temperature at calving, with no differences. Rumination time declined prepartum, reaching its lowest at 2 DIM in DI cows. It was significantly affected by days around calving, postpartum disorders, and THI. Activity increased prepartum and normalized by 4 DIM in HE cows, while DI cows showed higher activity at 4 DIM, stabilizing by 5–7 DIM. These findings underscore the value of precision monitoring tools for early disease detection and intervention. Full article

This review highlights a paradigm shift in our understanding of hypocalcemia during milk fever by introducing the concept of the Calci-Inflammatory Network. Traditionally viewed as a pathological deficiency necessitating rapid correction (e.g., through calcium borogluconate infusions or dietary adjustments like dietary cation-anion difference), periparturient hypocalcemia is reinterpreted here as an adaptive, protective response. Within this new framework, reduced circulating calcium levels may help temper systemic inflammation by limiting lipopolysaccharide (LPS) aggregation and curbing excessive macrophage activation. The review discusses how calcium signaling, the calcium-sensing receptor (CaSR), and immune cell functions adapt under hypocalcemic conditions to modulate inflammatory processes. This integrated perspective not only redefines the role of hypocalcemia but also proposes the Calci-Inflammatory Network as a novel concept through which we can understand how changes in calcium homeostasis mitigate inflammatory cascades—potentially lowering the incidence of periparturient diseases and enhance overall cow health and farm productivity. Future research should investigate the long-term effects of hypocalcemia, the environmental influences on this Calci-Inflammatory Network, and their collective impact on disease susceptibility and inflammation. Full article

In dairy cow, the peripartum metabolic stage is critical as may affect post-partum metabolic and reproductive recovery, colostrum quality, and overall reproductive fitness. This study aimed to evaluate the effects of varying doses of α-ketoglutarate (AKG) on lactation performance, reproductive performance, immune function, and antioxidant capacity in periparturient dairy cows. A total of 180 periparturient dairy cows were randomly assigned to four groups, with each cow receiving 1 g, 5 g, or 10 g of AKG in their prepartum diets. Results indicated that feeding 5 g and 10 g of AKG significantly increased the colostrum fat and protein content, reduced somatic cell counts, and improved daily milk yield. Regarding reproduction, AKG supplementation regulated reproductive hormones, increased postpartum estrogen levels, improved conception rates, and shortened the interval between breeding periods. For immune and antioxidant functions, AKG significantly increased serum IL-10 levels while reducing TNF-α and interleukins 1β and 6. It also significantly elevated glutathione peroxidase (GSH-PX) levels, reducing oxidative stress and demonstrating anti-inflammatory and immunomodulatory effects. Additionally, cows receiving medium-to-high doses of AKG had a significantly lower incidence of postpartum diseases such as mastitis. In conclusion, appropriate AKG supplementation can improve lactation performance, reproductive performance, immune function, and overall health in periparturient dairy cows, providing a theoretical basis for its use in dairy cow nutrition management. Full article

Milk fever, or periparturient hypocalcemia, in dairy cows has traditionally been addressed as an acute calcium deficiency, leading to interventions like supplementation and adjustments in dietary cation–anion balance. Although these measures have improved clinical outcomes, milk fever remains a widespread and economically significant issue for the dairy industry. Emerging findings demonstrate that a narrow emphasis on blood calcium concentration overlooks the complex interactions of immune, endocrine, and metabolic pathways. Inflammatory mediators and bacterial endotoxins can compromise hormone-driven calcium regulation and induce compensatory calcium sequestration, thereby worsening both clinical and subclinical hypocalcemia. Recent insights from systems biology illustrate that milk fever arises from nonlinear interactions among various physiological networks, rather than a single deficiency. Consequently, this review contends that a holistic strategy including integrating nutrition, immunology, microbiology, genetics, and endocrinology is vital for comprehensive management and prevention of milk fever. By embracing a multidisciplinary perspective, producers and veterinarians can develop more robust, customized solutions that not only safeguard animal well-being but also bolster profitability. Such an approach promises to meet the evolving demands of modern dairy operations by reducing disease prevalence and enhancing overall productivity. Tackling milk fever through integrated methods may unlock possibilities for improved herd health and sustainable dairy farming. Full article

This study aimed to evaluate the association between colostrum quality, as indicative of the immune status of the cows, and peripartum disease risk occurrence in a herd of grazing dairy cows. A prospective cohort study was conducted on a grazing dairy farm with 3000 Holstein milking cows from 15 March 2022 to 15 March 2023. Multivariable binary logistic models evaluated the association between colostrum quality (expressed in Brix grades as a continuous predictor) and the risk of peripartum diseases (including dystocia, stillbirth, retention of fetal membranes, metritis, and clinical endometritis). We found that colostrum quality was negatively associated with the risk of dystocia (p = 0.02), stillbirth (p < 0.01), clinical endometritis (p = 0.02), and total peripartum diseases (p < 0.01). Conversely, colostrum quality was not related to the risk of retention of the placenta (p = 0.25) or metritis (p = 0.76). Additionally, we found that the calving season affected colostrum quality (p < 0.01). Conversely, parity number, dry period length, and milk yield in previous lactation did not affect it (p > 0.10). We concluded that colostrum quality, expressed in Brix grades, is negatively associated with peripartum disease risk in dairy cows and could be a useful indicator of the immune status of the cow. Full article

Background/Objectives: The transition from a non-lactating to a lactating state is a critical period for lipid metabolism in dairy cows. Danggui Buxue Tang (DBT), stimulating energy metabolism, ameliorates diseases related to lipid metabolism disorders and is expected to be an effective supplement for alleviating excessive lipid mobilisation in periparturient dairy cows. This study aimed to investigate the effects of supplemental DBT on serum biochemical indices, faecal microbial communities, and plasma metabolites in dairy cows. Methods: Thirty cows were randomly divided into three groups: H-DBT group, L-DBT group, and control group. DBT administration was started on the day of calving and continued once daily for seven days. Faecal and blood samples were collected on calving day, 7 days after calving, and 14 days after calving. The levels of serum biochemical indices were measured at three time points in the three groups using commercial kits. Cows in the H-DBT group and control group were selected for metabolome and 16S rRNA amplicon sequencing. Results: Our research shows that, in dairy cows 7 days postpartum, DBT significantly reduced serum 3-hydroxybutyric acid (BHB) concentrations and the number of cows with BHB concentrations ≥ 1 mmol/L. Additionally, DBT increased serum total cholesterol contents at both 7 and 14 days postpartum. Analysis of the microbiota community showed that DBT modulated the composition and structure of the hindgut microbiota. Metabolomic analysis revealed decreased plasma acetylcarnitine, 2-hydroxybutyric acid, and BHB levels 7 days postpartum, whereas the TCA cycle was enhanced. At 14 days postpartum, DBT altered the plasma bile acid profile, especially glycine-conjugated bile acids, including GCDCA, GUDCA, and GDCA. Correlation analyses showed that the relative abundances of Bacillus, Solibacillus, Dorea, and Romboutsia were strongly correlated with the differential metabolites, which is crucial for the beneficial effects of DBT. Conclusions: DBT improves energy status and lipid metabolism in postpartum dairy cows by modulating hindgut microbiota and serum lipid metabolites. Full article

Metabolic diseases are significant diseases that affect the welfare, health, and production of small ruminant flocks raised for dairy and meat purposes. In breeding females, they mainly occur from six to eight weeks before and after parturition, respectively. Pregnancy toxemia and lactational ketosis are manifestations of hyperketonemia, primarily due to energetic deficit. Hypocalcemia and hypomagnesemia are related to the metabolic unavailability of calcium and magnesium, respectively. This review aimed to identify and discuss the current and most relevant aspects related to individual and herd health management of these interrelated metabolic diseases with impact on the sheep and goats’ farm sustainability. These diseases are primarily due to nutritional deficits, but homeostatic and homeorhetic disruptions are responsible for clinical signs and forms. Currently, their clinical diagnosis and monitoring are mainly assessed by biochemistry of body fluids and feed bromatological evaluation. Epidemiological studies and measuring risk factors also contribute to their prevention. Nevertheless, research on specific biomarkers and composite indices related to these diseases, in the context of herd health management and precision medicine, are new pathways driven to suitable and efficient animal production. Full article

The negative energy balance occurring in the periparturient period of cows will impede their health and postpartum performance. To target this issue, L-tryptophan was supplied to the prepartum cows. The results showed that L-tryptophan supplementation significantly increased the serum melatonin level and was accompanied with increases in SOD activity, IL-10 and colostrum IgA levels as well as decreases in MDA and IL-6 levels compared to the control cows. The incidence of postpartum diseases was significantly lower and the pregnancy rate was significantly higher in cows fed L-tryptophan than in the control group. A striking observation was that prepartum L-tryptophan supplementation not only improved the milk production but also the quality compared to the control cows. In general, supplementation with L-tryptophan in the prepartum period can improve the postpartum reproduction and lactation performance of cows to some extent. Full article

Hyperketonemia is common in cows postpartum and is associated with a decrease in milk production, reproductive efficiency, and increased risk of periparturient diseases and early culling from the herd. The objective of this research was to determine if feeding an exogenous source of propionate increased milk and milk component yield and reduced the incidence of hyperketonemia and other health events in Holstein dairy cows. Cows were systematically enrolled in the control group (C) or sodium propionate treatment group (SP) in a randomized block design. A subset of cows was sampled for blood glucose and betahydroxybutyrate (BHB) concentrations in milk at 3, 7, and 14 days using a NovaMax^® Plus™ meter (Nova Diabetes Care, Inc., Billerica, MA, USA). Data were analyzed using a mixed model. Average blood BHB and glucose concentrations during the postpartum period did not differ between treatments for multiparous or primiparous cows (C = 0.53 ± 0.02, SP: 0.55 ± 0.02 mmol BHB/L, p = 0.5; C = 44.0 ± 0.77, SP = 43.0 ± 0.78 glucose mg/dL, p = 0.6). However, the prevalence of hyperketonemia and metritis was high in primiparous cows (C = 35.6% and 19.8%, respectively; SP = 35.8% and 18.9%, respectively). Blood glucose was inversely related to BHB concentration for cows below 40 mg/dL blood glucose. Feeding sodium propionate during the postpartum period increased milk fat yield (C = 1.71; SP = 1.86 kg/day, p = 0.01), tended to increase milk yield in multiparous cows (C = 39.3; SP = 40.5 kg/day, p = 0.06) and increased milk fat yield in primiparous cows (C = 1.18; SP = 1.27 kg/day, p = 0.02). Including sodium propionate in the total mixed ration is beneficial to reduce health events and increase milk fat production in multiparous cows but may only increase milk fat production in primiparous cows. Full article

The use of individual calving pens in modern farming is widely recognized as a good practice for promoting good animal welfare during parturition. However, determining the optimal time to move a pregnant cow to a calving pen can be a management challenge. Moving cows too early may result in prolonged occupancy of the pen, while moving them too late may increase the risk of calving complications and production-related diseases. In this paper, a simple random walk type Markov Chain Model to predict the optimal time for moving periparturient cows to individual calving pens was proposed. Behavior changes such as lying time, standing time, and rumination time were analyzed using a video monitoring system, and we formulated these changes as the states of a Markov Chain with an absorbing barrier. The model showed that the first time entering an absorbing state was the optimal time for a pregnant cow to be moved to a calving pen. The proposed method was validated through a series of experiments in a real-life dairy farm, showing promising results with high accuracy. Full article

Membrane transporters and ion channels that play an indispensable role in metabolite trafficking have evolved to operate in Earth’s gravity. Dysregulation of the transportome expression profile at normogravity not only affects homeostasis along with drug uptake and distribution but also plays a key role in the pathogenesis of diverse localized to systemic diseases including cancer. The profound physiological and biochemical perturbations experienced by astronauts during space expeditions are well-documented. However, there is a paucity of information on the effect of the space environment on the transportome profile at an organ level. Thus, the goal of this study was to analyze the effect of spaceflight on ion channels and membrane substrate transporter genes in the periparturient rat mammary gland. Comparative gene expression analysis revealed an upregulation (p < 0.01) of amino acid, Ca²⁺, K⁺, Na⁺, Zn²⁺, Cl⁻, PO₄³⁻, glucose, citrate, pyruvate, succinate, cholesterol, and water transporter genes in rats exposed to spaceflight. Genes associated with the trafficking of proton-coupled amino acids, Mg²⁺, Fe²⁺, voltage-gated K⁺-Na⁺, cation-coupled chloride, as well as Na⁺/Ca²⁺ and ATP-Mg/Pi exchangers were suppressed (p < 0.01) in these spaceflight-exposed rats. These findings suggest that an altered transportome profile contributes to the metabolic modulations observed in the rats exposed to the space environment. Full article

Mitochondrial complex I inhibitor (iC1) is a methylation-controlled J protein (MCJ) that decreases cellular respiration by inhibiting oxidative phosphorylation. Recent rodent studies showed that loss or inhibition of iC1 was associated with preventing lipid accumulation. A common metabolic disorder of dairy cattle is a fatty liver disease (FLD), which often occurs during the periparturient period. In humans and rodents, iC1 is expressed in the liver and acts as a mitochondrial “brake”. However, iC1 expression in bovine liver and its possible role in FLD development have not yet been characterized. We hypothesized that iC1 is expressed in the bovine liver and that the expression of iC1 is correlated with FLD in periparturient dairy cattle. To test this hypothesis, we collected bovine liver tissue samples from an abattoir and isolated primary hepatic cells immediately following harvest. Utilizing an in vitro model of bovine FLD developed in our laboratory, we cultured primary hepatic cells in low-glucose DMEM supplemented with 10% FBS. The basal media was made to induce lipid accumulation and cytotoxicity in the primary liver cells with three treatments. To the basal media (control) we added 0.4 mM palmitate (treatment 1) or 20 ng/mL TNFα (treatment 2), or both 0.4 mM palmitate and 20 ng/mL TNFα (treatment 3). Consistent with our hypothesis, we present the novel characterization of iC1 expression in primary bovine liver cells cultured with or without the addition of lipotoxic factors made to emulate bovine FLD. We demonstrate both in situ and in vitro expression of iC1 in bovine liver and mRNA expression in hepatic cells and in the precipitates of conditioned media. The results of RT-qPCR, IHC, and western blot all demonstrated the expression of iC1 in bovine liver. In addition, we isolated precipitates of conditioned media further demonstrated iC1 expression by RT-qPCR. The transcript of iC1 tended to be more concentrated (4-fold; p > 0.05) in TNFα-treated conditioned media when compared with the control. Taken together, we present the novel finding that iC1 transcript and protein are expressed in liver tissue from dairy cattle, primary hepatic cells isolated from that liver tissue, and, finally, in the conditioned media derived from those cells. These novel findings and the prior findings on the role of iC1 in rodents and humans indicate that further investigation of the role of iC1 in the etiology and pathology of FLD in periparturient dairy cows is warranted. Full article

Mastitis is an inflammation of the mammary gland initiated by pathogenic bacteria. In fact, mastitis is the second most important reason for the culling of cows from dairy herds, after infertility. In this review we focus on various forms of mastitis, including subclinical and clinical mastitis. We also stress the importance of the dry-off period as an important time when pathogenic bacteria might start their insult to the mammary gland. An important part of the review is the negative effects of mastitis on milk production and composition, as well as economic consequences for dairy farms. The two most important groups of bacteria that are involved in infection of the udder, Gram-negative and Gram-positive bacteria, are also discussed. Although all cows have both innate and adaptive immunity against most pathogens, some are more susceptible to the disease than others. That is why we summarize the most important components of innate and adaptive immunity so that the reader understands the specific immune responses of the udder to pathogenic bacteria. One of the most important sections of this review is interrelationship of mastitis with other diseases, especially retained placenta, metritis and endometritis, ketosis, and laminitis. Is mastitis the cause or the consequence of this disease? Finally, the review concludes with treatment and preventive approaches to mastitis. Full article

Mycoplasma bovis (M. bovis) is an important pathogen affecting cattle, causing various diseases including pneumonia which mainly occurring in calves. Control of M. bovis infections is difficult due to the lack of commercial vaccines in most parts of the world and increasing trends of antimicrobial resistance in field isolates of the pathogen; therefore, it seems reasonable to look for new solutions for the prevention of the infection. Pegbovigrastim is a pegylated form of naturally occurring circulating cytokine in cattle that affects bovine leukocytes and some cell functions. Most studies on pegbovigrastim have focused on reducing the occurrence of mastitis and other diseases occurring during the periparturient period in cows, while this study attempts to use pegbovigrastim in the prevention of respiratory diseases in calves, which are largely caused by M. bovis. Based on previous observations on the immunostimulatory properties of pegbovigrastim in cattle, for the first time, the effect of its injection on the number and phagocytic and oxidative burst activities of peripheral blood granulocytes and monocytes in calves experimentally infected with M. bovis was investigated. Pegbovigrastim administration in the calves significantly stimulated an increase in peripheral blood granulocyte and monocyte counts and phagocytic activity of the cells, especially granulocytes, which was also generally expressed in the course of M. bovis infection. In response to pegbovigrastim administration, a general increase in the oxygen burst activity of the cells was observed. This effect was also shown despite ongoing infection with M. bovis which, taken together, may indicate a beneficial effect of pegbovigrastim injection on the immunity of the affected animals. Full article