Search Results (605)

Microbial degradation of pectin is a fundamental process for the carbon cycle and a strategic approach for treating industrial residues. This study characterizes a novel marine bacterium, Paenarthrobacter sp. FR1, isolated from East China Sea intertidal sediment, which exhibits the ability to utilize pectin. Its draft genome (4.83 Mb, 62.92% GC content) is predicted to encode 4498 protein-coding genes. Genomic analysis revealed a rich repertoire of Carbohydrate-Active Enzymes (CAZymes) crucial for this process, including 108 glycoside hydrolases (GHs), 7 polysaccharide lyases (PLs), 35 carbohydrate esterases (CEs), and 11 auxiliary activities (AAs). Genomic analysis provides supportive evidence that FR1 may target both homogalacturonan (HG) and rhamnogalacturonan (RG) pectin domains, potentially through complementary hydrolytic and oxidative pathways. Phylogenomic analysis based on Average Nucleotide Identity (ANI, 83.56%) and digital DNA-DNA Hybridization (dDDH, 27.8%) confirmed its status as a potential novel species. Notably, FR1 is a rare Paenarthrobacter isolate with innate pectinolytic capability, a characteristic not previously documented in this genus. This strain’s unique enzymatic machinery highlights its importance in marine carbon cycling and provides a valuable biotechnological resource for degrading pectin-rich wastes. Full article

The effects of pectin extraction, cellulose purification, and sonication on the juice extraction residue from grapefruit were investigated. Pectin extraction using pressurized carbon dioxide (pCO₂) in a sodium oxalate solution (U-OX) lowered the cellulose content and increased the hemicellulose and lignin contents, whereas pectin extraction in deionized water (U-DW) did not affect these contents. Pectin extraction and cellulose purification induced hydrolysis and removal of non-crystalline cellulose regions. The sonication of the purified cellulose samples formed fiber-like structures with widths of <100 nm on their surfaces. The cellulose purification process increased the surface charge and formed a gel-like structure with increased hardness, adhesiveness, and film structure. These processes enhance the absorption of amphiphilic dyes, although to a lesser extent than that of the untreated juice extraction residue (UJR) after sonication. Before sonication, UJR adsorbed cationic dyes, whereas after, UJR adsorbed both polar and nonpolar dyes. These results suggest that juice residue could be used as a biomaterial with diverse potential applications. Full article

Texture is a critical quality attribute of strawberry fruit, and phytohormones play a pivotal role in fruit softening, which mainly results from cell wall metabolism, which is governed by genes and enzymes. To gain further insights into strawberry (Fragaria × ananassa, Duch. cv.  Akihime ) softening, our study investigated changes across five stages in fruits in their firmness, soluble solids content (SSC), cell microstructure, cell wall materials, activities of cell wall-modifying enzymes, gene expression, endogenous phytohormone levels, and their correlation. During strawberry ripening, firmness decreased, while SSC, intercellular space, and separation of the cell wall from the plasma membrane increased. Meanwhile, the contents of ionic pectin (ISP) and cellulose (CE), pectin methylesterase (PME) activity, FaPME expression, and the levels of zeatin (Z) and strigolactone (SL) decreased, showing a positive correlation with firmness. In contrast, the activities of pectate lyase (PL) and cellulase (Cx), the expression of FaPL and FaCx, and the contents of gibberellin A₄ (GA₄), GA₉, and abscisic acid (ABA) increased during ripening, and these were negatively correlated with firmness. These results suggest that Z and SL are associated with the maintenance of cell wall integrity and firmness, whereas increases in GA₄, GA_9, and ABA are linked to enhanced cell wall disassembly and fruit softening. Full article

Natural fillers have been widely explored to enhance the mechanical and barrier properties of biodegradable films. In this study, a mineral-rich powder obtained from the solid components of Ucides cordatus crab shells was processed (washing, drying, milling, and sieving at 75 µm) and extensively characterized using SEM, FTIR XRD, EDX, mineral analysis, hygroscopicity, density, and particle size distribution. The powder exhibited heterogeneous morphology and contained 22.52 g·kg⁻¹ of calcium carbonate, along with other trace minerals; its crystalline profile indicated the presence of both calcite and aragonite. Low hygroscopicity (1.76%) and a true density of 2.11 g/cm³ were also observed. When incorporated into pectin-based films at 1–5%, the filler promoted a reduction in film thickness, indicating enhanced structural compaction. Solubility increased linearly with filler content, whereas water vapor permeability (WVP) decreased at 1% and 2% but rose again at 4% and 5%, correlating positively with solubility (r = 0.895). Films containing 4% and 5% exhibited higher tensile strength and elastic modulus, confirming increased rigidity. At elevated concentrations, the films also became less luminous and more chromatic. Overall, the findings demonstrate that crab-shell mineral powder is a viable and sustainable reinforcement capable of tailoring the structural, mechanical, and barrier performance of biodegradable films. Full article

The purpose of this paper is to develop sustainable bio-colored healthcare cotton fabrics using chitosan/pectin layer-by-layer coatings enriched with strawberry pomace extract. Ultrasound-assisted extraction of bioactive compounds from strawberry pomace was optimized using a Box–Behnken experimental design to maximize the total polyphenol content (29.6 mg GAE/g DW). HPLC analysis confirmed the presence and quantity of anthocyanins in the extract obtained under optimized conditions (time: 37 min, ethanol concentration: 48.4%, liquid-to-solid ratio: 40.4 mL/g). This extract was used to enrich chitosan/pectin coatings, and applied onto cotton fabric using a layer-by-layer technique. Among the fabrics with 0, 4, 8, and 12 bilayers of chitosan/pectin coating enriched with extract, the 12-bilayer coated fabric exhibited the best bioactivity: 98.4% antioxidant activity, outstanding antibacterial efficacy, and the highest release of extract over 24 h. The last two properties showed a strong linear correlation with the number of bilayers, adsorbed extract, and weight gain. In vitro cytotoxicity testing proved the non-cytotoxic nature of 12-bilayer fabric extract. Additionally, the performed 12-bilayer coating imparted a natural reddish-brown color, high color strength (K/S = 1.45), and excellent UV protection (UPF 79.4), offering a sustainable, multifunctional approach for obtaining bio-colored cotton fabrics suited for wound dressing and other healthcare applications. Full article

Growing environmental concerns and the need for sustainable materials have accelerated the search for biodegradable alternatives to food packaging. Since nearly half of global plastic production is dedicated to food packaging, and less than 5% is recyclable, developing eco-friendly solutions is urgent. Biopolymeric films enriched with microalgae and cyanobacteria have emerged as promising options due to their bioactive properties. This study screened 38 film-forming formulations combining different biopolymers with varying concentrations of Spirulina (0–5%) to identify the most suitable candidates based on physical and visual characteristics. Films produced with pectin and hydroxypropylmethylcellulose (HPMC) matrices were selected for detailed characterization, including physicochemical, optical, mechanical, thermal, barrier, surface, and functional group analyses, as well as antioxidant activity. The highest elongation at break (%) was observed in the control HPMC film (16.5 ± 3.85), whereas the lowest value was recorded for the pectin film containing 1% Spirulina (2.75 ± 0.49). In parallel, the highest thickness (mm) was found in the pectin film with 5% Spirulina (0.153 ± 0.018), while the lowest thickness occurred in the HPMC film incorporating 1% biomass (0.076 ± 0.004). The incorporation of Spirulina decreased solubility and moisture content while increasing opacity. HPMC-based films demonstrated superior mechanical strength, thermal stability, barrier performance, and significantly higher antioxidant activity compared to pectin films. Antioxidant activity increased with biomass concentration, peaking at 5% (HPMC: 320.08 ± 35.7 µmol TE/g; pectin: 36.92 ± 7.63 µmol TE/g). Overall, the HPMC film containing 1% Spirulina showed the best balance of properties, including mechanical behavior and antioxidant performance, indicating strong potential for food packaging applications, particularly for protecting light-sensitive and oxidation-prone foods. Full article

The objective of this study was to develop a pectin–tetraethoxysilane (TEOS) hybrid gel with improved functional properties and biocompatibility. The sol–gel process was used to create pectin–TEOS hydrogels containing 0.75, 1.00, 1.25, and 1.50 M TEOS, which were labeled AP-T0.75, AP-T1.00, AP-T1.25, and AP-T1.50. The pectin–TEOS hydrogel AP-T1.50 exhibited a hardness of 631 kPa, a Young’s modulus of 1588 kPa, and an elasticity of 1.95 mm. The degree of swelling decreased as the TEOS content increased. The pectin–TEOS hydrogel AP-T1.25 exhibited the highest strength of adhesion to serosa of 60.6 mN. Serum protein and bovine serum albumin (BSA) adsorption by pectin–TEOS gels was recorded in the range of 2–43 µg/mg after 6 h of incubation at pH 5.0, 7.4, and 8.0. Pectin–TEOS gels demonstrated low rates of hemolysis and complement activation. Leukocyte adhesion on the surface of pectin–TEOS gels depends on TEOS content. Consequently, the mechanical characteristics, serosal adherence, and biocompatibility of pectin–TEOS gel position it as a strong contender for the advancement of smart biomaterials. Full article

Orange peel is suitable for reuse due to the quantity and variety of bioactive compounds it contains, such as pectins, sugars and hesperidin. This study designed a scheme for reusing orange peel extract (OPE) using membrane technologies. Initially, a 100 kDa ceramic membrane was used to separate the pectins and hesperidine from acids and sugars and obtain a clarified product. In the subsequent stage, two ultrafiltration membranes of 25 and 5 kDa were tested, improving the results in terms of product transmittance and obtaining permeates whose physical–chemical parameters are compatible with those established by the European Fruit Juice Association. These membranes did not achieve complete separation of monosaccharide sugars from disaccharides. Finally, a 200 Da nanofiltration membrane was used, which completely reduced the sucrose and pectin content, concentrating glucose and fructose by 40%, values higher than those obtained with the GR90PP membrane. In addition, calcium and magnesium ions were completely rejected. Color changes in the permeate and concentrate streams could be appreciated due to the high concentration produced when working in batches. The nanofiltration (NF) process obtained lower yields (approximately 30%) compared to ultrafiltration (approximately 85%). Full article

Fruit peel cracking significantly reduces the commercial value of melons (Cucumis melo). To elucidate the underlying mechanisms of peel cracking, we conducted integrated investigations including morphological, physiological, metabolomic and transcriptomic analyses of cracked and non-cracked peels from the crack-resistant ‘Xizhoumi 17’ and crack-susceptible ‘Xizhoumi 25’ cultivars. The parenchyma cells in ‘Xizhoumi 17’ exhibited a compact and well-organized arrangement, whereas those in ‘Xizhoumi 25’ displayed a loosely packed and disordered structure. Notably, cracked peels exhibited significantly higher levels of water-soluble pectin and lignin, along with increased cellulase, polygalacturonase, catalase, superoxide dismutase, and peroxidase activities. In contrast, protopectin, cellulose, and hemicellulose contents, as well as polyphenol oxidase activity, were markedly reduced compared to non-cracked peels. Metabolomic analysis revealed that the phenylpropanoid biosynthesis pathway is positively correlated with the progression of peel cracking. RNA-seq analysis revealed 119 and 82 differentially expressed genes associated with cell wall metabolism and lignin biosynthesis pathways, respectively. Collectively, these findings underscore the involvement of genes related to cell wall synthesis and degradation, as well as lignin synthesis, in modulating peel cracking through alterations in cell wall composition and structural stability, thereby offering practical implications for reducing melon peel cracking incidence via targeted molecular breeding of key genes regulating cell wall composition and the phenylpropanoid pathway. Full article

This study aimed to develop a nutraceutical gummy candy enriched with hydrolysed hemp (Cannabis sativa L.) as a natural antioxidant and antihypertensive ingredient. Three European cultivars—Futura 75, Henola, and KC Zuzana—were cultivated under rainfed (RF) and irrigated (RFCI) conditions and assessed for nutritional composition and bioactivity. Henola variety showed the most favourable profile, showing the highest protein content under RFCI (29.4 g 100 g⁻¹ d.m.) and the greatest phenolic concentration under RF (15.8 µmol GAE g⁻¹ d.m.), with 35–40% higher antioxidant capacity than the other cultivars. Henola (RF) was selected for enzymatic hydrolysis with Ultraflo^® XL, which enhanced total phenolics and antioxidant capacity by 65% and 58%, respectively, stabilizing after 18 h. Incorporation of the hydrolysate (0.66%) into a pectin-based gummy significantly (p < 0.05) increased total phenolic content by 52% and antioxidant capacity by up to 60% compared with controls. After simulated digestion, bioactivity decreased by 30–45% but remained higher than controls. The incorporation of 0. 66 g of hydrolysed ingredient in 100 g of gummy increased ACE inhibition by 10% after digestion, probably associated with the peptides released during the digestion, confirming hydrolysed hemp as a stable multifunctional ingredient for plant-based nutraceutical formulations targeting oxidative stress and hypertension. Full article

Passiflora ligularis (granadilla), widely cultivated in Colombia, contains secondary metabolites such as flavonoids, phenols, and pectins. Owing to their strong water-retention capacity, pectins are promising candidates for moisturizing cosmetic formulations. This study optimized pectin extraction from fruit peel and mesocarp using aqueous reflux at 90 °C and acid extraction with citric or hydrochloric acid (0.25 N and 0.125 N) at 40–60 °C. The effects of solvent, method (reflux or microwave-assisted), time (15–25 min), and temperature (50–60 °C) were investigated. Extracted pectins were dried, lyophilized, and incorporated into eight gel-type cosmetic formulations subjected to seven-day preliminary stability testing (physicochemical and organoleptic evaluation). Optimal extraction was achieved with citric acid under microwave irradiation at 60 °C for 15 min, yielding 45.23%. The pectin exhibited low moisture (0.13%), acidity (0.42%), methoxyl content (9.05%), and degree of esterification (57.6%), along with high swelling capacity (12.46 mL/g) and water-retention capacity (12.26%). The resulting gel formulation was homogeneous and stable. In vitro assays confirmed significant moisturizing activity. These findings highlight P. ligularis pectins as sustainable biopolymers with potential as natural gelling and moisturizing agents in cosmetic products. Full article

The pathogenic fungus Mycocentrospora acerina, responsible for Panax notoginseng round spot disease, poses a serious threat to the development of the P. notoginseng industry. To investigate its genetic information and potential pathogenic mechanisms, this study employed nanopore third-generation sequencing technology to conduct de novo genome sequencing and analysis of M. acerina, followed by an assessment of its plant cell wall-degrading enzyme activities. The sequencing results revealed that the M. acerina genome has a total length of 37.03 Mb, a GC content of 47.68%, an N50 value of 1.66 Mb, and a repeat sequence proportion of 9.37%. A total of 9989 protein-coding genes were predicted. Genome annotation identified 499 carbohydrate-active enzyme (CAZyme) family genes—more than those found in Botrytis cinerea (469), Phanerochaete chrysosporium (381), and Erysiphe necator (136). Moreover, M. acerina harbors a relatively large number of genes encoding plant cell wall-degrading enzymes. Experimental measurements of cell wall-degrading enzyme activities were consistent with the genomic predictions, demonstrating that M. acerina exhibits strong abilities to degrade cellulose, pectin, and lignin. This study provides new insights into the pathogenic mechanisms of M. acerina and establishes a theoretical foundation for developing potential control strategies for P. notoginseng round spot disease. Full article

The dragon fruit (Selenicereus sp.) peel is a viable plant source for the extraction of polysaccharides such as pectin, the demand for which has increased significantly in the food and pharmaceutical industries. In Nayarit, Mexico, the Queen Purple variety of dragon fruit (Selenicereus cf. guatemalensis) is commonly cultivated. The peel is typically discarded, while only the pulp is utilized for direct consumption or processed into derivative products. The objective of this study was to characterize the properties of pectin extracted from the peel of dragon fruit (Selenicereus cf. guatemalensis ‘Queen Purple’). The yield, molecular weight, anhydrouronic acid content, betalain content, antioxidant capacity, and phenolic compounds were determined using gravimetric, volumetric, spectrophotometric, and colorimetric techniques, among others. Furthermore, the functional groups and degree of esterification of the pectin were identified using Fourier-transform infrared spectroscopy. The pectin presented a yield of 12.8%, esterification degree of 49.85%, molecular weight of 645 kDa, anhydrouronic acid, phenolic acid and betalain content of 98.27%, 195.7 mg EAG/100 gDW and 4.26 mg/100 gDW respectively and an antioxidant capacity of 149.6, 192.76 and 20.5 mg EAA/100 gDW by the DPPH, ABTS and FRAP methods respectively, classified as high-purity, low-methoxyl, intermediate-molecular-weight, with an important betalain content and antioxidant capacity. Based on these findings, the extracted pectin complies with the Food and Agriculture Organization specifications and shows promise as a functional ingredient in the food industry. Full article

This study investigated the impact of two different temperature regimes (high and low) on the chemical composition, antioxidant activity, and antidiabetic properties of mango jellies. Total phenolic content (TPC) and antioxidant capacity were assessed using the conventional 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and the recently developed direct current (DC) polarographic method. Jellies prepared under low-temperature conditions contained higher TPC levels (82.0 ± 2.0 mg gallic acid equivalents (GAE)/kg jelly) and exhibited stronger antioxidant activity (65.0 ± 2.2 ascorbic acid equivalents (AAE)/100 g jelly by DPPH; 12.40 × 10⁻⁶ mol reduced Hg(II)/g jelly by DC polarography). Antidiabetic evaluation revealed that the low-temperature jelly significantly inhibited both α-amylase and α-glucosidase activity. Thermal analysis further confirmed distinct structural behavior between low- and high-temperature products. This novel processing approach—combining mild heating (55 °C), vacuum treatment, and reduced sugar content (40%) without pectin addition—proved effective in preserving bioactive compounds and functionality. Notably, this is the first report applying DC polarography to assess antioxidant capacity in fruit jellies, highlighting its potential as a robust tool in functional food research. Full article

Peach trees (Prunus persica L. Batsch) produce climacteric fruits that are prone to senescence and softening after harvesting, and they are susceptible to external pathogens that cause rot and deterioration. This study investigated the effects of 1-methylcyclopropene (1-MCP) treatment combined with laser microporous film (LMF) packaging on the preservation of firm-fleshed honey peaches (‘Xiahui No. 8’ variety) during refrigerated storage at 5 ± 1 °C. The combined 1-MCP + LMF treatment significantly reduced respiration and rot rates and preserved the levels of reducing sugar and titratable acid after 35 days more effectively compared to the control and LMF groups. The 1-MCP + LMF packaging suppressed cell-wall-degrading enzymes (polygalacturonase, β-glucosidase, and cellulase) and maintained high contents of original pectin, cellulose, and hemicellulose. The treatment also reduced the accumulation of superoxide anions and malondialdehyde, maintained cell-wall structural integrity and fruit hardness, and delayed fruit browning by inhibiting polyphenol oxidase and peroxidase activity. Together, our results demonstrate that the combination of 1-MCP treatment and LMF packaging effectively preserved the hardness and quality of firm-fleshed honey peaches during refrigerated storage, extending their shelf life to 28 days while maintaining good sensory and nutritional qualities. Full article