Search Results (151)

In this study, the impacts of traditional processing on phytates contents, phytate: mineral molar ratios, and the bioaccessibility of calcium, iron, and zinc in three traditional koko production units (KP1, KP2, and KP3) and two zoomkoom production units (ZP1 and ZP2) products were assessed based on the variations in their traditional processing techniques. The total calcium content of ZP1 was ranked the highest (58.02 mg/100 g, p < 0.05) compared to other processed samples. A high total value of iron (17.76 mg/100 g, p < 0.05) was revealed among koko compared to zoomkoom. Whereas KP3 and ZP2 showed the highest (p < 0.05) amount of zinc (3.34 mg/100 g). ZP1 showed a calcium bioaccessibility of 6.3% (p < 0.05). The iron bioaccessibility was within the average range of 5–30%, with KP1 ranking the highest (21.8%), while ZP1 showed the highest value (42.2%) (p < 0.05) in bioaccessibility of zinc among the zoomkoom products. The processing techniques adopted caused up to a 56.7% to 76.76% reduction (p < 0.05) of phytic acid in the pearl millet, leading to a decrease in the molar ratios of [Ca]:[Phy], [Fe]:[Phy], and [Phy]:[Zn]. However, the phytic acid content varied among the koko and zoomkoom, corresponding with the varied inhibitory mechanism indices reported. In brief, a positive correlation was shown between the traditional processing techniques, phytate, and in vitro bioaccessibility of minerals, indicating the consumption of koko and zoomkoom as a good source of functional minerals. Full article

The aim was to evaluate the productivity, agronomic characteristics, and chemical and mineral composition of pearl millet genotypes irrigated with brackish water under the application of agricultural gypsum in two cuts. The experiment was a randomized block design in a 4 (gypsum levels—0, 2, 4, and 8 ton ha⁻¹ applied on the surface) × 3 (pearl millet genotypes—ADR 300, BRS 1501, and IPA BULK 1BF) factorial arrangement, with three replications, irrigated with high brackish water and low sodium. Agricultural gypsum had no significant effect on productivity, agronomic characteristics, and chemical and mineral composition (p > 0.05). In the first cut, higher mean values were found for the percentage of panicle, crude protein, ether extract, in vitro dry matter digestibility, calcium, sulfur, and manganese (p < 0.05). For the second cut, higher results were observed for green matter productivity, dry matter productivity, water use efficiency, stem percentage, stem diameter, average leaf size, panicle size, acid detergent fiber, lignin, cellulose, total carbohydrates, potassium, and copper (p < 0.05). IPA Bulk 1 BF showed a larger panicle size in both cuts (p < 0.05). The evaluated pearl millet genotypes showed desirable agronomic characteristics and tolerance to irrigation with brackish water regardless of gypsum application, thus they are indicated for cultivation in the semi-arid regions. Full article

Mycorrhizal-mediated seedling establishment may reduce dependency on chemical fertilizers, but the effectiveness of infection for growth may differ depending on species with different eco-physiological adaptations. The infection of arbuscular mycorrhizal fungi (AMF) and P uptake were compared between rice (Oryza sativa L.) (Koshihikari (rice_k), Togo4 (rice_t)), and pearl millet (Pennisetum glaucum (L.) R. Br.) (ICMB89111 (millet₈₉₁), ICMB95444 (millet₉₅₄)) seedlings (i) in response to three different commercial AMF inoculants of Rhizoglomus irregulare (popular inoculant Dr. Kinkon (I₁); two new inoculants Rootella P (I₂) and Rootella F (I₃)) in comparison with indigenous AMF from Andosol upland and paddy topsoils (Exp. 1–2 as the inoculant experiments) and (ii) across different water regimes from upland to flooded lowland conditions for I₁ inoculant (Exp. 3–4 as the water regime experiments). The new inoculants I₂ and I₃ with higher propagule numbers showed a higher infection rate than the control seedlings in both rice and pearl millet, with a tendency for slower leaf development and no seedling growth enhancement. I₁ inoculant had more significant positive effects on the root transversal area and shoot growth parameters than the control. The infection rates of all three inoculants were lower than the indigenous AMF from upland Andosol in rice and pearl millet, in which a higher infection rate led to higher P uptake found in millet₉₅₄. I₁ inoculant increased the infection rate in pearl millet and rice but had no clear indication of interaction with water regimes. A higher infection rate led to higher P uptake and shoot dry weight in pearl millet but not in rice with higher root length density. This study provided the significance of inoculants for seedling establishment and highlighted more mycorrhizal-mediated P uptake in pearl millet than in rice. Full article

Pearl millet is a cereal crop vital for food security in Africa and Asia. It is widely adapted for dual-purpose production, providing grain for human consumption and fodder for livestock, particularly during dry seasons. This study aimed to evaluate three dual-purpose pearl millet genotypes from Namibia and South Africa while exploring prospects for future production practices. Growth analysis is essential for quantitatively assessing crop growth, development, and production. A growth analysis study was conducted by collecting and evaluating weather data, water use efficiency, and crop growth parameters that are valuable for modelling, allowing for observing and quantifying strengths and weaknesses between varieties for food and fodder or as dual-purpose varieties. The analysis focused on water use, plant height, fractional radiation interception, panicle number, tiller number, flowering date, stem diameter, panicle length, dry matter distribution, harvest index, grain yield, and panicle diameter, under well-watered, supplementary irrigation, and rainfed conditions. The landrace achieved a higher yield under well-watered conditions than the hybrid and improved varieties. The hybrid pearl millet produced a greater fodder yield than the improved short variety in well-watered and water-limited treatments. The improved variety suits grain production, whereas the landrace and hybrid are more suitable for dual-purpose production. The landrace performed well in rainfed and irrigated situations across the three seasons. The landrace (Kantana) recorded the highest grain yield (1.01 kg m⁻²), followed by the hybrid (Agrigreen) (0.97 kg m⁻²), while the improved variety (Kangara) had the lowest grain yield (0.74 kg m⁻²). Full article

Millets are climate-resistant, potential alternatives to wheat that could provide environmental, food security, and health benefits (e.g., lower glycemic index). However, millets are high in phytic acid, which reduces the bioavailability of essential minerals. Millets are often fermented in Africa and parts of Asia to improve bioavailability and, thus, nutritional value, but both unfermented and fermented millets may have flavors unfamiliar to Western cultures. We conducted two pilot studies on sensory perception and liking of whole grain, United States pearl millet (Pennisetum glaucum), in a group of U.S. consumers. In a preliminary study, we compared pearl millet treated under five different conditions (0, 48, and 96 h of fermentation fully submerged in either distilled water or in a 5% NaCl solution at 28 °C). We found that 96 h of spontaneous fermentation in water, an inexpensive and accessible technique consistent with consumer demand for minimally processed foods, reduced phytic acid by ~72%. However, consumers (n = 12) rated flatbreads made with fermented pearl millet as more bitter and sour than flatbreads made with unfermented pearl millet. In a second study, participants (n = 30) rated liking and purchase intent for whole wheat bread with 0 to 50% (w/w) substitution of pearl millet flour. Replacing up to 20% of wheat with fermented or unfermented pearl millet had no measurable effect on liking or purchase intent. More extensive substitution compromised liking, particularly with fermented pearl millet. More work is needed, but so far, there appear to be no sensory barriers to at least partial substitution of whole-grain pearl millet for wheat in whole wheat bread for United States consumers. Full article

Weaned Angus-cross steers (n = 69; body weight [BW] = 233 ± 29 kg) were used for the following purposes: (1) develop an assay to measure 3-methylhistidine (3MH) in blood samples to monitor changes in protein turnover, and (2) evaluate the effects of an implant (Revalor-G, 40 mg of trenbolone acetate, 8 mg of estradiol) or an implant plus individual supplementation via SuperSmart Feeder (SSF; C-Lock Inc., Rapid City, SD, USA) on animal growth, muscle growth, and nitrogen retention patterns in backgrounded beef steers. Steers were blocked by weight, trained to precision supplementation via SSF, and then assigned to one of three treatments: (1) grazing only [G], (2) grazing with implant [GI], or (3) grazing with implant plus individual animal supplementation via SSF at 0.75% BW [GIS] for 56 d of grazing study. Steers grazed a cowpea and pearl millet mixture until d 42, when forage became limiting, and steers were transitioned to an oat and annual ryegrass baleage. Steers were weighed and blood was collected every 14 d to determine plasma urea nitrogen (PUN), creatinine (CREAT), and 3-methylhistidine (3MH) concentrations. Ultrasound measurements of ribeye area (REA) were collected on d 0, 28, and 56. Data were analyzed using the GLIMMIX with treatment, day, and two-way interaction in the model. A novel, robust method for the determination of 3MH was developed and validated. The development of this 3MH method allows us to monitor changes in protein turnover in cattle over time, a metric which is related to production efficiency. A treatment-by-day interaction (p = 0.0050) was observed for BW and REA. Steer BW did not differ (p > 0.05) on d 0, 14, or 28 between treatments; however, on d 42, GI and GIS steers had a greater (p < 0.01) BW than G steers. On d 56, BW was greater (p < 0.0001) for GIS than GI and G, which not differ (p > 0.05). Total BW gain during the 56 d study was greater (p < 0.001) by 54% for GIS compared to GI or G. For GI, the total BW gain was greater (p < 0.01) by 18% compared to G. On d 56, the REA of GI and GIS steers was greater (p < 0.001) than of G steers. Steers in the GI treatment group had a greater PUN and PUN–CREAT ratio concentrations than the GIS steers. Both G and GI steers had greater 3-methylhistidine (3MH) concentrations compared to GIS steers on d 28. Our results demonstrate that the supplementation of implanted steers improves growth and improves nitrogen utilization during backgrounding on forage diets. This research illustrates that animal and muscle growth may be limited in grazing animals implanted with combination implants (estrogenic and androgenic compounds) when supplementation is not included. Full article

Crop simulation models serve as effective instruments for evaluating the management conditions of irrigation systems. This study aims to simulate maize production to identify optimal irrigation water management strategies under deficit irrigation and moisture conservation practices, utilizing the AquaCrop model. We conducted this research at Woleh irrigation schemes during the 2023/2024 irrigation season in the Wag-himra zone of northern Ethiopia. To check how well the model worked, we used statistical tests such as prediction error (PE), root mean square error (RMSE), index of agreement (D), goodness-of-fit (R²), and the Nash–Sutcliffe coefficient of efficiency (NCE). The model effectively simulated canopy cover, aboveground biomass, and yield across all treatments, evidenced by the high R² (0.99) and NSE (0.99) values. Furrow-irrigated raised bed planting (FRBP) at 100% and 75% ETc with mulch exhibited the lowest predicted errors and deviations in yield and water productivity. The model effectively predicted maize yield and biomass under full irrigation in FRBP at 75% ETc with mulch. The AquaCrop model serves as a dependable measure of maize crop development and outcomes across different irrigation conditions and mulch types, potentially enhancing sustainable maize productivity in water-stressed areas. Full article

Pearl millet (Pennisetum glaucum (L.) R. BR.) is a C4 plant adapted to semi-arid climates and is one of the primary staple foods in Sub-Saharan Africa, including in Namibia. The decline in yields associated with water scarcity over the years has been a national concern in the country. An experimental field trial was conducted at the Mannheim Crop Research Station, Namibia, during the 2023 and 2024 cropping seasons to investigate the response of two local pearl millet cultivars (Kangara and Okashana 2) to different water regimes (100%, 75%, and 50% crop evapotranspiration [ETc]) according to morpho-physiological and yield parameters. Pearl millet was planted in a split-plot factorial design with four rows per plot under the three water regimes, and the genotypes were planted in subplots. The results revealed that the water regime had a significant effect on plant height, number of leaves, tillers, chlorophyll content, stomatal conductance, leaf temperature, stem thickness, number of productive tillers, panicle diameter, panicle length, dry panicle weight, biomass, grain weight, and 1000-seed weight of the two pearl millet cultivars (p < 0.001). At 50% ETc, the water regime significantly reduced the growth and yield parameters compared with the 75% ETc and 100% ETc water regimes, highlighting the significance of water in plant development and growth. The findings highlighted that both cultivars responded similarly to water stress. Seventy-five percent of ETc is recommended to be applied in pearl millet systems in semi-arid conditions. This research has significant implications for the planning and producing of pearl millet under water-limited environments under changing climatic conditions. Full article

The cover crop Pennisetum glaucum (L.) R.Br. (pearl millet) reduces the emergence of weed species in the field through a mechanism that is not fully known. The identification of the allelopathic activity of pearl millet can contribute to the development of no-tillage techniques to produce crops without or with low doses of herbicides. This issue was investigated by testing the effects of extracts from the aerial parts of pearl millet on the germination and growth of the weeds Bidens pilosa L., Euphorbia heterophylla L., and Ipomoea grandifolia (Dammer) O’Donell under laboratory conditions. The ethyl acetate fraction (EAF) at a concentration of 2000 µg mL⁻¹ was inactive on Bidens pilosa; it inhibited root length (−72%) and seedling fresh weight (−41%) of E. heterophylla, and in I. grandifolia the length of primary root and aerial parts and the fresh and dry weight of seedlings were reduced by 63%, 32%, 25%, and 12%, respectively. In roots of I. grandifolia seedlings, at the initial development stage, EAF induced oxidative stress and increased electrolyte leakage. At the juvenile vegetative stage, a lower concentration of EAF (250 µg mL⁻¹) induced a stimulus in seedling growth (+60% in root length and +23% in aerial parts length) that was associated with increased photosynthetic efficiency. However, at higher concentrations (1000 µg mL⁻¹), it induced the opposite effects, inhibiting the growth of root (−41%) and aerial parts (−25%), with reduced superoxide dismutase activity and photosynthetic efficiency. The stilbenoid pallidol was identified as the main compound in EAF. The allelopathic activity of pearl millet may be attributed, at least in part, to the impairment of energy metabolism and the induction of oxidative stress in weed seedlings, with pallidol possibly involved in this action. Such findings demonstrated that the application of the EAF extract from pearl millet can be a natural and renewable alternative tool for weed control. Full article

Protein and minerals are central to human diets. This study investigated the effects of germination periods (24 h, 48 h, and 72 h) as a pretreatment process on the nutritional and antinutritional components of pearl millet and amaranth grain flours (28 ± 0.1 °C). The results showed significant differences (p < 0.05) in the proximate, mineral, and antinutrient factors of the samples. The highest protein content, 8.61% in the millet and 17.37% in the amaranth, was observed after 24 h of germination. Specifically, the protein content in the millet after 24 h of germination, 8.61%, was significantly higher than after 72 h, which was 8.07%, and also higher than in the ungerminated millet flour, which was 7.71%. Furthermore, the concentration of iron (Fe) in the millet after 24 h, 48 h, and 72 h of germination was 4.77 mg/100 g, 4.90 mg/100 g, and 4.96 mg/100 g, while in the amaranth; they were 4.10 mg/100 g, 5.86 mg/100 g, and 5.89 mg/100 g. The iron concentration in the ungerminated millet flour, 3.31 mg/100 g, was significantly lower than what was observed in the germinated millet across the periods. A similar trend was observed in the amaranth. In terms of antinutrients, a notable reduction in their concentration was observed as the germination periods increased. The concentration of phytates in the millet ranged from 0.173 g to 0.836/100 g. However, the phytate concentration significantly decreased from 0.836 g/100 g in the ungerminated millet flour to 0.326 g/100 g, 0.230 g/100 g, and 0.173 g/100 g after 24 h, 48 h, and 72 h of germination, respectively. This study shows the potential influence of germination periods on the quality of grains such as millet and amaranth. Full article

Agricultural soil environments contain different types of nematodes in all trophic levels that aid in balancing the soil food web. Beneficial free-living nematodes (FLNs) consist of bacterivores, fungivores, predators, and omnivores that help in the mineralization of the soil and the top-down control of harmful plant-parasitic nematodes (PPNs). Annually, USD 125 billion in worldwide crop losses are caused by PPNs, making them a plant pathogen of great concern for growers. Farmers have started to implement the use of cover crops in agricultural systems for the protection and enrichment of soil but research on how different cover crops affect nematode populations is lacking and in demand. This study aims to determine the effects of legume and grass cover crops, Cowpea (Vigna unguiculata) and Pearl Millet (Pennisetum glaucum), as well as their mixture on the abundance and diversity of FLN and PPN populations. Soil samples were collected at the time of cover crop maturity and spinach harvest to analyze nematode communities using both morphological and DNA metabarcoding analysis. The results showed that the application of Cowpea and Pearl Millet as well as their mixture in a spinach agricultural system led to the control of PPNs and proliferation of FLN communities, with each cover crop treatment demonstrating different advantages for the various nematode feeding groups. Soil property analysis did not show a significant difference except for magnesium and total nitrogen levels, which were significantly correlated with nematode community composition. The overall findings of our study indicate that the choice of cover crop implementation by growers for spinach cultivation should be based on specific soil health conditions, which in turn promote soil fertility and a healthy nematode community. Full article

In tropical regions, maintaining crop residues in the soil is challenging. Silicon (Si) may increase the persistence of these residues in the soil, as it is a precursor to lignin, providing a gradual release of nutrients for subsequent crops. Therefore, the objective of this study was to evaluate the influence of different doses of calcium silicate (Ca₂SiO₄) (0, 1, 2, and 3 Mg ha⁻¹) and limestone (0, 1, 2, and 3 Mg ha⁻¹) on the lignin content, residue decomposition, and nutrient release of four cover crops—Pennisetum glaucum, Urochloa ruziziensis, Crotalaria spectabilis, and Cajanus cajan—at various decomposition stages following cover crop management (0, 30, 60, 90, and 120 days). The experiment was conducted in the field at the experimental area of the Faculty of Engineering at Ilha Solteira-UNESP, located in the municipality of Selvíria, state of Mato Grosso do Sul, on Ferralsol. The decomposition rate of the residues was assessed using the decomposition bag method, which was installed after cover crop management. The concentrations of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), Si, lignin, and cellulose were determined. Silicate application did not affect the accumulation of nutrients by cover crops and their release into the soil. There was no relationship between the remaining Si in the dry matter of plants and more persistent residues. The most persistent plants had higher final dry matter lignin content. Using pearl millet and pigeon peas resulted in more persistent residues in the soil. Full article

The main challenge of the no-tillage system (NTS) is to reconcile productivity, the maintenance of surface residues, and the stabilization of soil organic matter (SOM). To address this challenge, particularly in tropical regions, various cover crops have been tested. The objective of this study was to test the effects of agricultural crop succession systems on the stock and stability of soil organic carbon in different surface layers of the soils. The research was carried out in the state of Goiás, Brazil, in an experiment set up in 2016, designed in randomized blocks with a split-plot scheme (treatments and soil layers), comprising four repetitions (blocks). The treatments (plots) consisted of crops grown in succession to soybean, which were as follows: T1—soybean/corn (Zea mays); T2—soybean/pearl millet (Pennisetum glaucum); T3—soybean/Urochloa ruziziensis (brachiaria); and T4—corn + Urochloa ruziziensis. The subplots represented the following soil layers: 0–5, 5–10, 10–20, and 20–40 cm. We evaluated the biomass dry mass and the soil parameters such as soil density, total porosity, and light organic matter across all layers. The organic carbon, grain size fractionation (mineral-associated organic carbon—MOC; sand-sized carbon—POC), and isotopic composition (δ13C) were determined in the 0–5 and 5–10 cm layers. The highest biomass dry production was observed in the soybean/pearl millet succession, which reduced the soil density and increased the total porosity in the surface layer. The soybean/pearl millet treatment produced high amounts of light organic matter, particularly in the 0–5 cm layer, a result also found for the soybean/brachiaria and soybean/corn + brachiaria systems. The crop successions did not alter the soil carbon stock or stability; however, the surface layer stored the highest amount of carbon, with elevated total organic carbon values and carbon stocks and stability (MOC and POC). Overall, in this study, replacing corn with other crops in succession with soybean did not affect the stock or stability of soil organic carbon. The species grown in succession with soybean contributed to the higher surface carbon stock and stability, promoting the formation of more stable and recalcitrant carbon. Full article

Different cropping systems and nutrient management techniques impact the microbiological characteristics of soil and nutrient availability for plants. This study assessed four cropping systems—rice–wheat, cotton–wheat, pearl millet–wheat, and pearl millet–mustard in Hisar district, Haryana, using 80 soil samples (20 from each system) collected in April 2022 after the Rabi crop harvest. The cotton–wheat system had the highest accessible nitrogen (N) at 155.9 kg ha⁻¹, while both the cotton–wheat (59.3 kg ha⁻¹) and rice–wheat (54.0 kg ha⁻¹) systems had higher available sulfur (S) levels compared to pearl millet–wheat (41.2 kg ha⁻¹). Pearl millet–wheat also showed 12.4% higher potassium (K) levels than rice–wheat. The rice–wheat system exhibited the highest phosphorus (P) concentration at 54.3 kg ha⁻¹ and greater DTPA-extractable micronutrients. Soils from the rice–wheat system had higher DTPA-extractable micronutrients (Zn, Fe, Mn, Cu) and superior microbial biomass nitrogen (MBN, 54.7 mg kg⁻¹), urease (37.9 µg NH₄⁺-N g⁻¹ h⁻¹), and alkaline phosphatase activity (APA, 269.7 µg PNP g⁻¹ h⁻¹) compared to other systems. Canonical discriminant functions explained 88.1% of the variability among cropping systems, while principal component analysis identified available P, DTPA-extractable Zn, and Cu as key soil quality indicators, accounting for 66.9% of the variance. These insights can inform policymakers on promoting effective cropping systems and sustainable soil health in northwestern India. Full article

Foliar blast, caused by Pyricularia grisea, poses a major challenge to pearl millet (Pennisetum glaucum (L.) R. Br) production, leading to severe yield losses, particularly in rainfed ecologies. This study aimed to elucidate the genetic basis of blast resistance through a genome-wide association study (GWAS) involving 281 diverse pearl millet inbreds. GWAS panel was phenotyped for blast resistance against three distinct isolates of P. grisea collected from Delhi, Gujarat, and Rajasthan locations, revealing a significant variability with 16.7% of the inbreds showing high resistance. Bayesian information and linkage disequilibrium iteratively nested keyway (BLINK) and Multi-Locus Mixed Model (MLMM) models using transformed means identified 68 significant SNPs linked to resistance, with hotspots for resistance-related genes on chromosomes 1, 2, and 6. These regions harbor genes involved in defense mechanisms, including immune response, stress tolerance, signal transduction, transcription regulation, and pathogen defense. Genes, namely 14-3-3-like proteins RGA2, RGA4, hypersensitive-induced response proteins, NHL3, NBS-LRR, LRR-RLK, LRRNT_2, and various transcription factors such as AP2/ERF and WRKY, played a crucial role in the stress-responsive pathways. Analyses of transporter proteins, redox processes, and structural proteins revealed additional mechanisms contributing to blast resistance. This study offers valuable insights into the complex genetic architecture of blast resistance in pearl millet, offering a solid foundation for marker-assisted breeding programs and gene-editing experiments. Full article