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  • Open Access
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12 Pages

Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events

  • Alexander B. Stilgoe,
  • Declan J. Armstrong and
  • Halina Rubinsztein-Dunlop

The trap stiffness us the key property in using optical tweezers as a force transducer. Force reconstruction via maximum-likelihood-estimator analysis (FORMA) determines the optical trap stiffness based on estimation of the particle velocity from sta...