Search Results (57)

In recent years, researchers have significantly increased their exploration of nanomaterials, primarily due to their exceptional and distinctive electrochemical properties. Hierarchical nanostructured materials have become a prevalent component in electrochemical sensors owing to their numerous advantages, including abundant open diffusion channels, diverse junction interfaces, and a highly exposed surface area. This review provides a comprehensive overview of the potential of hierarchical nanomaterials as electrode modifiers, highlighting their capacity to enhance device performance. The introduction section sets the context by addressing the challenges and recent advancements in the field of hierarchical nanomaterials, emphasizing their promise for electrochemical sensor applications, and outlining the diverse research directions that are currently being explored. In the following section, a range of strategies and techniques for synthesizing hierarchical nanomaterials are outlined, with an emphasis on the impact of various parameters on their properties. Subsequently, the characteristics and performance of diverse hierarchical nanomaterials as electrode modifiers for electrochemical sensor applications are examined. Ultimately, the primary aspects and challenges of hierarchical nanomaterials in the domain of electroanalysis are reported, followed by a discussion of their future development. Full article

This review article focuses first on the historical development of present understanding of gap junction channel architecture, one of its goals being to enlighten younger generations of scientists about the early steps of this field that begun over half a century ago. Early findings on gap junction architecture are reviewed as follows. The channels cross the membrane and project from the membrane surfaces; they are made of six subunits (hexamers) and show dimples on both ends, which represent inner and outer openings of the channel. Images of the central dimples on both channel ends (channel pores) seen in freeze-fracture replicas correspond to the electron-opaque spots visible in negatively stained sections and in isolated junctions. The channels are linked to each other extracellularly. Calmodulin (CaM) is a major accessory protein of gap junctions that is involved in channel gating and gap junction formation and is also likely to play a key role in determining different patterns of channel aggregation. Full article

Understanding flow dynamics in open-channel node systems is crucial for designing effective hydraulic engineering solutions and minimizing energy losses. This study investigates how junction geometry—specifically the lateral inflow angle (α = 45° and 60°) and the longitudinal bed slope (I = 0.0011 to 0.0051)—influences the water velocity distribution and hydraulic losses in a rigid-bed Y-shaped open-channel junction. Experiments were performed in a 0.3 m wide and 0.5 m deep rectangular flume, with controlled inflow conditions simulating steady-state discharge scenarios. Flow velocity measurements were obtained using a PEMS 30 electromagnetic velocity probe, which is capable of recording three-dimensional velocity components at a high spatial resolution, and electromagnetic flow meters for discharge control. The results show that a lateral inflow angle of 45° induces stronger flow disturbances and higher local loss coefficients, especially under steeper slope conditions. In contrast, an angle of 60° generates more symmetric velocity fields and reduces energy dissipation at the junction. These findings align with the existing literature and highlight the significance of junction design in hydraulic structures, particularly under high-flow conditions. The experimental data may be used for calibrating one-dimensional hydrodynamic models and optimizing the hydraulic performance of engineered channel outlets, such as those found in hydropower discharge systems or irrigation networks. Full article

Various nature-based solutions (NbS)—such as constructed wetlands, drainage ditches, and vegetated buffer strips—have recently demonstrated strong potential for mitigating pollutant transport in open channels and river systems. Numerical modeling is a widely adopted and effective approach for assessing the performance of these interventions. This study presents the first development of a two-dimensional (2D) meshless advection–diffusion model based on an Eulerian smoothed particle hydrodynamics (SPH) framework, specifically designed to simulate passive pollutant transport in open channel flows. The proposed model marks a pioneering application of the ESPH technique to environmental pollutant transport problems. It couples the 2D depth-averaged shallow water equations with an advection–diffusion equation to represent both fluid motion and pollutant concentration dynamics. A uniform particle arrangement ensures that each fluid particle interacts symmetrically with eight neighboring particles for flux computation. To represent the pollutant transport process, the dispersion coefficient is defined as the sum of molecular and turbulent diffusion components. The turbulent diffusion coefficient is calculated using a prescribed turbulent Schmidt number and the eddy viscosity obtained from a Smagorinsky-type mixing-length turbulence model. Three analytical case studies, including one-dimensional transcritical open channel flow, 2D isotropic and anisotropic diffusion in still water, and advection–diffusion in a 2D uniform flow, are employed to verify the model’s accuracy and convergence. The model demonstrates first-order convergence, with relative root mean square errors (RRMSEs) of approximately 0.2% for water depth and velocity, and 0.1–0.5% for concentration. Additionally, the model is applied to a laboratory experiment involving 2D pollutant dispersion in a 90° junction channel. The simulated results show good agreement with measured velocity and concentration distributions. These findings indicate that the developed model is a reliable and effective tool for evaluating the performance of NbS in mitigating pollutant transport in open channels and river systems. Full article

α-Latrotoxin (αLTX) causes exhaustive release of neurotransmitters from nerve terminals in the absence of extracellular Ca²⁺ (Ca²⁺_e). To investigate the mechanisms underlying this effect, we loaded mouse neuromuscular junctions with BAPTA-AM. This membrane-permeable Ca²⁺-chelator demonstrates that Ca²⁺_e-independent effects of αLTX require an increase in cytosolic Ca²⁺ (Ca²⁺_cyt). We also show that thapsigargin, which depletes Ca²⁺ stores, induces neurotransmitter release, but inhibits the effect of αLTX. We then studied αLTX’s effects on Ca²⁺_cyt using neuroblastoma cells expressing signaling-capable or signaling-incapable variants of latrophilin-1, a G protein-coupled receptor of αLTX. Our results demonstrate that αLTX acts as a cation ionophore and a latrophilin agonist. In model cells at 0 Ca²⁺_e, αLTX forms membrane pores and allows the influx of Na⁺; this reverses the Na⁺-Ca²⁺ exchanger, leading to the release of stored Ca²⁺ and inhibition of its extrusion. Concurrently, αLTX stimulates latrophilin signaling, which depletes a Ca²⁺ store and induces transient opening of Ca²⁺ channels in the plasmalemma that are sensitive to inhibitors of store-operated Ca²⁺ entry. These results indicate that Ca²⁺ release from intracellular stores and that Ca²⁺ influx through latrophilin-activated store-operated Ca²⁺ channels contributes to αLTX actions and may be involved in physiological control of neurotransmitter release at nerve terminals. Full article

Effective Ohmic contact between metals and their conductive channels is a crucial step in developing high-performance Ga₂O₃-based transistors. Distinct from bulk materials, excess thermal energy of the annealing process can destroy the low-dimensional material itself. Given the thermal budget concern, a feasible and moderate solution (i.e., Ar-containing plasma treatment) is proposed to achieve effective Ohmic junctions with (100) β-Ga₂O₃ nanosheets. The impact of four kinds of plasma treatments (i.e., gas mixtures SF₆/Ar, SF₆/O₂/Ar, SF₆/O₂, and Ar) on (100) β-Ga₂O₃ crystals is comparatively studied by X-ray photoemission spectroscopy for the first time. With the optimal plasma pre-treatment (i.e., Ar plasma, 100 W, 60 s), the resulting β-Ga₂O₃ nanosheet field-effect transistors (FETs) show effective Ohmic contact (i.e., contact resistance R_C of 104 Ω·mm) without any post-annealing, which leads to competitive device performance such as a high current on/off ratio (>10⁷), a low subthreshold swing (SS, 249 mV/dec), and acceptable field-effect mobility (${\mu }_{\mathrm{e}\mathrm{f}\mathrm{f}}$, ~21.73 cm² V⁻¹ s⁻¹). By using heavily doped β-Ga₂O₃ crystals (N_e, ~10²⁰ cm⁻³) for Ar plasma treatments, the contact resistance R_C can be further decreased to 5.2 Ω·mm. This work opens up new opportunities to enhance the Ohmic contact performance of low-dimensional Ga₂O₃-based transistors and can further benefit other oxide-based nanodevices. Full article

(1) Background: The main factors and their interrelationships contributing to cardiac repolarization alternans (CRA) remain unclear. This study aimed to elucidate the calcium (Ca²⁺)-related mechanisms underlying myocardial ischemia (MI)-induced CRA. (2) Materials and Methods: CRA was induced using S1 stimuli for pacing in an in silico ventricular model with MI. The standard deviations of nine Ca²⁺-related subcellular parameters among heartbeats from 100 respective nodes with and without alternans were chosen as features, including the maximum systole and end-diastole and corresponding differences in the Ca²⁺ concentration in the intracellular region([Ca²⁺]_i) and junctional sarcoplasmic reticulum ([Ca²⁺]_jsr), as well as the maximum opening of the L-type Ca²⁺ current (I_CaL) voltage-dependent activation gate (d-gate), maximum closing of the inactivation gate (ff-gate), and the gated channel opening time (GCOT). Feature selection was applied to determine the importance of these features. (3) Results: The major parameters affecting CRA were the differences in [Ca²⁺]_i at end-diastole, followed by the extent of d-gate activation and GCOT among beats. (4) Conclusions: MI-induced CRA is primarily characterized by functional changes in Ca²⁺ re-uptake, leading to alternans of [Ca²⁺]_i and subsequent alternans of I_CaL-dependent properties. The combination of computational simulation and machine learning shows promise in researching the underlying mechanisms of cardiac electrophysiology. Full article

This study investigates the computational fluid dynamics (CFD) modeling of supercritical open channel junction flow using two different turbulence models: k-ω shear stress transport (SST) and k-ω SST scale-adaptive simulation (SAS), in conjunction with Volume of Fluid (VOF) and mixture multiphase models. The efficacy of these models in predicting the intricate free surface fluctuation and free surface elevation in a supercritical junction is evaluated through a comprehensive analysis of time-averaged free surface data obtained from CFD simulations and Light Detection and Ranging (LIDAR) measurements. The dimensionless Reynolds (Re) and Froude (Fr) numbers of the investigated scenario were Fr = 9 and Re = 5.1 × 10⁴ for the main channel, and Fr = 6 and Re = 3.3 × 10⁴ for the side channel. The results of the analysis demonstrated a satisfactory level of agreement with the experimental data. However, certain limitations associated with both CFD and LIDAR were identified. Specifically, the CFD performance was limited by the model’s incapacity to consider small-scale turbulent effects and to model air bubbles smaller than the cell size while the LIDAR measurements were limited by instrument range, inability to provide insight into what is happening below the water surface, and blind spots. Nonetheless, the k-ω SST turbulent model with the VOF multiphase model most closely matched the LIDAR results. Full article

Connexin hemichannels (HCs) expressed at the plasma membrane of mammalian cells are of paramount importance for intercellular communication. In physiological conditions, HCs can form gap junction (GJ) channels, providing a direct diffusive path between neighbouring cells. In addition, unpaired HCs provide conduits for the exchange of solutes between the cytoplasm and the extracellular milieu, including messenger molecules involved in paracrine signalling. The synergistic action of membrane potential and Ca²⁺ ions controls the gating of the large and relatively unselective pore of connexin HCs. The four orders of magnitude difference in gating sensitivity to the extracellular ([Ca²⁺]_e) and the cytosolic ([Ca²⁺]_c) Ca²⁺ concentrations suggests that at least two different Ca²⁺ sensors may exist. While [Ca²⁺]_e acts as a spatial modulator of the HC opening, which is most likely dependent on the cell layer, compartment, and organ, [Ca²⁺]_c triggers HC opening and the release of extracellular bursts of messenger molecules. Such molecules include ATP, cAMP, glutamate, NAD⁺, glutathione, D-serine, and prostaglandins. Lost or abnormal HC regulation by Ca²⁺ has been associated with several diseases, including deafness, keratitis ichthyosis, palmoplantar keratoderma, Charcot–Marie–Tooth neuropathy, oculodentodigital dysplasia, and congenital cataracts. The fact that both an increased and a decreased Ca²⁺ sensitivity has been linked to pathological conditions suggests that Ca²⁺ in healthy cells finely tunes the normal HC function. Overall, further investigation is needed to clarify the structural and chemical modifications of connexin HCs during [Ca²⁺]_e and [Ca²⁺]_c variations. A molecular model that accounts for changes in both Ca²⁺ and the transmembrane voltage will undoubtedly enhance our interpretation of the experimental results and pave the way for developing therapeutic compounds targeting specific HC dysfunctions. Full article

This study employs COMSOL software v 5.6 to investigate a novel approach to heat transfer via mixed convection in an open hollow structure with an unheated 90° baffle elbow. Two 20 W heat sources are strategically positioned on the cavity’s bottom and right-angled wall for this research. Notably, the orientation of the baffle perpendicular to the airflow is used to direct external, unrestricted flow into the square cavity. The research investigates a range of air velocities (0.1, 0.5, 1.0, and 1.5 m/s) and the intricate interaction between input air velocity, dual heated sources, and the presence of a right-angle baffle on critical thermodynamic variables, such as temperature distribution, isotherms, pressure variation, velocity profile, air density, and both local and mean Nusselt numbers. Validation of the applicable computational method is achieved by comparing it to two previous studies. Significant findings from numerical simulations indicate that the highest velocity profile is in the centre of the channel (2.3–2.68 m/s at an inflow velocity of 1.5 m/s), while the lowest profile is observed along the channel wall, with a notable disruption near the inlet caused by increased shear forces. The cavity neck temperature ranges from 380 to 640 K, with inflow air velocities varying from 0.1 to 1.5 m/s (Re is 812 to 12,182), respectively. In addition, the pressure fluctuates at the channel-cavity junction, decreasing steadily along the channel length and reaching a maximum at the intake, where the cavity neck pressure varies from 0.01 to 2.5 Pa with inflow air velocities changing from 0.1 to 1.5 m/s, respectively. The mean Nusselt number exhibits an upward trend as air velocity upon entry increases. The mean Nusselt number reaches up to 1500 when the entry air velocity reaches 1.5 m/s. Due to recirculation patterns, the presence of the 90° unheated baffle produces a remarkable cooling effect. The study establishes a direct correlation between input air velocity and internal temperature distribution, indicating that as air velocity increases, heat dissipation improves. This research advances our understanding of convective heat transfer phenomena in complex geometries and provides insights for optimising thermal management strategies for a variety of engineering applications. Full article

Motivated by the increasing need of optimised micro-devices for droplet production in medical and biological applications, this paper introduces an integrated approach for the study of the liquid–liquid droplet creation in flow-focusing micro cross-junctions. The micro-junction considered is characterised by a restriction of the channels cross-sections in the junction, which has the function of focusing the flow in the region of the droplet formation. The problem is studied numerically in the OpenFOAM environment and validated by a comparison with experimental results obtained by high-speed camera images and micro-PIV measurements. The analysis of the forces acting on the dispersed phase during the droplet formation and the diameter of the droplets obtained numerically are considered for the development of a model of the droplet breakup under the squeezing regime. On the basis of energy balancing during the breakup, a relation between interfacial tension, the size of the cross-sections in the junction, and the time interval needed for droplet creation is obtained, which yields a novel correlation between the dimensionless length of the droplet and the dimensionless flow rate. This research expands our knowledge of the phenomenon of drop creation in micro-junctions with restrictions providing new aid for the optimal design of micro-drop generators. Full article

Gap junction channels are regulated by gates sensitive to cytosolic acidification and trans-junctional voltage (Vj). We propose that the chemical gate is a calmodulin (CaM) lobe. The fast-Vj gate is made primarily by the connexin’s NH₂-terminus domain (NT). The chemical gate closes the channel slowly and completely, while the fast-Vj gate closes the channel rapidly but incompletely. The chemical gate closes with increased cytosolic calcium concentration [Ca²⁺]_i and with Vj gradients at Vj’s negative side. In contrast, the fast-Vj gate closes at the positive or negative side of Vj depending on the connexin (Cx) type. Cxs with positively charged NT close at Vj’s negative side, while those with negatively charged NT close at Vj’s positive side. Cytosolic acidification alters in opposite ways the sensitivity of the fast-Vj gate: it increases the Vj sensitivity of negative gaters and decreases that of positive gaters. While the fast-Vj gate closes and opens instantaneously, the chemical gate often shows fluctuations, likely to reflect the shifting of the gate (CaM’s N-lobe) in and out of the channel’s pore. Full article

Connexins (Cxs) form gap junctions through homotypic/heterotypic oligomerization. Cxs are initially synthesized in the endoplasmic reticulum, then assembled as hexamers in the Golgi apparatus before being integrated into the cell membrane as hemichannels. These hemichannels remain closed until they combine to create gap junctions, directly connecting neighboring cells. Changes in the intracellular or extracellular environment are believed to trigger the opening of hemichannels, creating a passage between the inside and outside of the cell. The size of the channel pore depends on the Cx isoform and cellular context-specific effects such as posttranslational modifications. Hemichannels allow various bioactive molecules, under ~1 kDa, to move in and out of the host cell in the direction of the electrochemical gradient. In this review, we explore the fundamental roles of Cxs and their clinical implications in various neurological dysfunctions, including hereditary diseases, ischemic brain disorders, degenerative conditions, demyelinating disorders, and psychiatric illnesses. The influence of Cxs on the pathomechanisms of different neurological disorders varies depending on the circumstances. Hemichannels are hypothesized to contribute to proinflammatory effects by releasing ATP, adenosine, glutamate, and other bioactive molecules, leading to neuroglial inflammation. Modulating Cxs’ hemichannels has emerged as a promising therapeutic approach. Full article

Hydrodynamic models are widely used in simulating water dynamics in riverine and estuarine systems. A reasonably realistic representation of the geometry (e.g., channel length, junctions, cross-sections, etc.) of the study area is imperative for any successful hydrodynamic modeling application. Typically, hydrodynamic models do not digest these data directly but rely on pre-processing tools to convert the data to a readable format. This study presents a parsimonious open-source and user-friendly Java software tool, the Cross-Section Development Program (CSDP), that is developed by the authors to prepare geometric inputs for hydrodynamic models. The CSDP allows the user to select bathymetry data collected in different years by different agencies and create cross-sections and computational points in a channel automatically. This study further illustrates the application of this tool to the Delta Simulation Model II, which is the operational forecasting and planning hydrodynamic and water quality model developed for the Sacramento–San Joaquin Delta in California, United States. Model simulations on water levels and flow rates at key stations are evaluated against corresponding observations. The simulations mimic the patterns of the corresponding observations very well. The square of the correlation coefficient is generally over 0.95 during the calibration period and over 0.80 during the validation period. The absolute bias is generally less than 5% and 10% during the calibration and validation periods, respectively. The Kling–Gupta efficiency index is generally over 0.70 during both calibration and validation periods. The results illustrate that CSDP can be efficiently applied to generate geometric inputs for hydrodynamic models. Full article

A flood protection dike blends seamlessly with natural surroundings. These dikes stand as vital shields, mitigating the catastrophic effects of floods and preserving both communities and ecosystems. Their design not only aids in controlling water flow but also ensures minimal disruption to the local environment and its biodiversity. The present study used a uniform cohesionless sand with d₅₀ = 0.9 mm to investigate the local scour process near a single combined dike (permeable and impermeable), replicating a flooding scenario. The experiments revealed that the maximum scour depth is likely to occur at the upstream edge of the dike, resembling a local scour observed around a scaled-down emerged dike in an open channel. The scour hole downstream of the dike gets shallower as it gets smaller, as do the horseshoe vortices that surround it. Additionally, by combining different pile shapes, the flow surrounding the dike was changed to reduce horseshoe vortices, resulting in scour length and depth reductions of 48% at the nose and 45% and 65% at the upstream and downstream dike–wall junction, respectively. Contrarily, the deposition height downstream of the dike had a reciprocal effect on permeability, which can severely harm the riverbank defense system. The combined dike demonstrates their ability to mitigate scour by reducing the flow swirls formed around the dike. The suggested solutions can slow down the rapid deterioration and shield the dike and other river training infrastructure from scour-caused failures. Full article