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Search Results (168)

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38 pages, 7157 KB  
Article
Research on Pedestrian Dynamics and Its Environmental Factors in a Jiangnan Water Town Integrating Video-Based Trajectory Data and Machine Learning
by Hongshi Cao, Zhengwei Xia, Ruidi Wang, Chenpeng Xu, Wenqi Miao and Shengyang Xing
Buildings 2025, 15(21), 3996; https://doi.org/10.3390/buildings15213996 - 5 Nov 2025
Abstract
Jiangnan water towns, as distinctive cultural landscapes in China, are confronting the dual challenge of surging tourist flows and imbalances in spatial distribution. Research on pedestrian dynamics has so far offered narrow coverage of influencing factors and limited insight into underlying mechanisms, falling [...] Read more.
Jiangnan water towns, as distinctive cultural landscapes in China, are confronting the dual challenge of surging tourist flows and imbalances in spatial distribution. Research on pedestrian dynamics has so far offered narrow coverage of influencing factors and limited insight into underlying mechanisms, falling short of a systemic perspective and an interpretable theoretical framework. This study uses Nanxun Ancient Town as a case study to address this gap. Pedestrian trajectories were captured using temporarily installed closed-circuit television (CCTV) cameras within the scenic area and extracted using the YOLOv8 object detection algorithm. These data were then integrated with quantified environmental indicators and analyzed through Random Forest regression with SHapley Additive exPlanations (SHAP) interpretation, enabling quantitative and interpretable exploration of pedestrian dynamics. The results indicate nonlinear and context-dependent effects of environmental factors on pedestrian dynamics and that tourist flows are jointly shaped by multi-level, multi-type factors and their interrelations, producing complex and adaptive impact pathways. First, within this enclosed scenic area, spatial morphology—such as lane width, ground height, and walking distance to entrances—imposes fundamental constraints on global crowd distributions and movement patterns, whereas spatial accessibility does not display its usual salience in this context. Second, perceptual and functional attributes—including visual attractiveness, shading, and commercial points of interest—cultivate local “visiting atmospheres” through place imagery, perceived comfort, and commercial activity. Finally, nodal elements—such as signboards, temporary vendors, and public service facilities—produce multi-scale, site-centered effects that anchor and perturb flows and reinforce lingering, backtracking, and clustering at bridgeheads, squares, and comparable nodes. This study advances a shift from static and global description to a mechanism-oriented explanatory framework and clarifies the differentiated roles and linkages among environmental factors by integrating video-based trajectory analytics with machine learning interpretation. This framework demonstrates the applicability of surveillance and computer vision techniques for studying pedestrian dynamics in small-scale heritage settings, and offers practical guidance for heritage conservation and sustainable tourism management in similar historic environments. Full article
(This article belongs to the Section Architectural Design, Urban Science, and Real Estate)
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20 pages, 5998 KB  
Article
Land Use Shapes the Rhizosphere Microbiome and Metabolome of Naturally Growing Barbarea vulgaris
by Emoke Dalma Kovacs and Melinda Haydee Kovacs
Metabolites 2025, 15(11), 684; https://doi.org/10.3390/metabo15110684 - 22 Oct 2025
Viewed by 350
Abstract
Background: Land use change fundamentally alters soil microbial communities and biochemical processes, yet the integrated effects on rhizosphere microbiome–metabolome networks remained poorly understood. Objective: This study investigated land uses as forest, grassland and intermediary edge shape the rhizosphere biochemical networks of naturally grown [...] Read more.
Background: Land use change fundamentally alters soil microbial communities and biochemical processes, yet the integrated effects on rhizosphere microbiome–metabolome networks remained poorly understood. Objective: This study investigated land uses as forest, grassland and intermediary edge shape the rhizosphere biochemical networks of naturally grown Barbarea vulgaris. Methods: Rhizosphere soils of Barbarea vulgaris were analysed for microbial community structure abundance, and metabolomic profile applying phospholipid fatty acid (PLFA) profiling and mass spectrometric untargeted metabolomics (GC–MS/MS and MALDI–TOF/TOF MS). These were coupled with co–inertia analysis to assess microbiome–metabolome interactions. Results: Microbial community analysis revealed significant effects of land use on bacterial community structure (G+/G−, p < 0.001). Untargeted metabolomics identified 248 metabolites, of which 161 were mapped to KEGG pathways. Amino acids and derivatives (21.1%) followed by organic acids (16.8%) were the most representative among identified metabolites. Pathway enrichment analysis revealed coordinated reprogramming of central carbon and nitrogen metabolism across land use gradients, particularly in the amino acid metabolism, TCA cycle, and glycolysis/gluconeogenesis pathways. Microbiome–metabolome coupling analysis revealed distinct correlation patterns between microbial phenotypes and metabolite classes, with forest environments showing the strongest biochemical network integration (RV = 0.91). Edge habitats presented intermediate signatures, supporting their role as transitional zones with unique biochemical properties. Conclusions: The environmental context fundamentally shapes rhizosphere biochemical network organization through coordinated shifts in bacterial community structure and metabolic pathway activity. These habitat-specific metabolic signatures suggest that land use change triggers adaptive biochemical responses that may influence plant performance and ecosystem functioning across environmental gradients. Full article
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22 pages, 1973 KB  
Article
Research on the Coupling Coordination Between the Development Level of China’s Construction Industry and Carbon Emissions
by Jiaqiang Ren, Yizhuo Wang and Chanyu Xu
Sustainability 2025, 17(16), 7501; https://doi.org/10.3390/su17167501 - 19 Aug 2025
Viewed by 798
Abstract
In the framework of global efforts to mitigate climate change and in alignment with the “Dual Carbon” objectives, the construction sector, a fundamental cornerstone of the national economy, has garnered significant attention concerning its development and carbon emissions. This study collected data from [...] Read more.
In the framework of global efforts to mitigate climate change and in alignment with the “Dual Carbon” objectives, the construction sector, a fundamental cornerstone of the national economy, has garnered significant attention concerning its development and carbon emissions. This study collected data from the construction sector across 30 Chinese provinces (including autonomous regions and municipalities) to develop an evaluation index system for assessing high-quality development. The random forest algorithm was utilized to assess the levels of high-quality development, whereas the carbon emission factor approach was used to quantify emissions at the provincial level. Subsequently, a coupling coordination model was employed to analyze the interrelationship between development levels and carbon emissions. Key findings indicate the following: (1) China’s construction sector has shown sustained improvement in high-quality development; however, significant regional disparities persist, with eastern provinces (e.g., Beijing, Jiangsu) outperforming their central and western counterparts (e.g., Guangxi, Guizhou). (2) Carbon emissions from the construction sector exhibited an M-shaped fluctuation pattern, characterized by an increase from 2013 to 2014, followed by a decline in 2015, a subsequent recovery from 2016 to 2019, a transient decrease in 2020, and an eventual rebound in 2021 and 2022. Spatially, the developed coastal provinces of Jiangsu and Zhejiang exhibited significantly higher carbon emissions compared to regions such as Hainan and Ningxia. (3) The coupling coordination degree indicated a gradual increase from 0.50 to 0.55 (mean values); however, 78% of provinces remained at the “barely coordinated” level (0.5 ≤ D < 0.6), leading to a notable spatial distribution that is marked by elevated values in the eastern and southern regions, while exhibiting reduced values in the western and northern areas. Regional divergence was observed through four characteristic evolutionary trajectories: eastern China exhibited a U-shaped recovery, western China maintained linear growth, central China experienced inverted V-shaped fluctuations, and northeast China displayed W-shaped oscillations. Full article
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25 pages, 2247 KB  
Article
The Impact of Selected Market Factors on the Prices of Wood Industry By-Products in Poland in the Context of Climate Policy Changes
by Anna Kożuch, Dominika Cywicka, Marek Wieruszewski, Miloš Gejdoš and Krzysztof Adamowicz
Energies 2025, 18(16), 4418; https://doi.org/10.3390/en18164418 - 19 Aug 2025
Viewed by 1396
Abstract
The objective of this study was to analyze price variability and the factors influencing the formation of monthly prices of by-products of the wood industry in Poland between October 2017 and January 2025. The analysis considered the impact of economic variables, including energy [...] Read more.
The objective of this study was to analyze price variability and the factors influencing the formation of monthly prices of by-products of the wood industry in Poland between October 2017 and January 2025. The analysis considered the impact of economic variables, including energy commodity prices (natural gas and coal) and industrial wood prices, on the pricing of wood industry by-products. The adopted approach enabled the identification of key determinants shaping the prices of these by-products. The effectiveness of two tree-based regression models—Random Forest (RF) and CatBoost (CB)—was compared in the analysis. Although RF offers greater interpretability and lower computational requirements, CB proved more effective in modeling dynamic, time-dependent phenomena. The results indicate that industrial wood prices exerted a weaker influence on by-product prices than natural gas prices, suggesting that the energy sector plays a leading role in shaping biomass prices. Coal prices had only a marginal impact on the biomass market, implying that changes in coal availability and pricing did not directly translate into changes in the prices of wood industry by-products. The growing role of renewable energy sources derived from natural gas and wood biomass is contributing to the emergence of a distinct market, increasingly independent of the traditional coal market. In Poland, due to limited access to alternative energy sources, biomass plays a critical role in the decarbonization of the energy sector. Full article
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17 pages, 2958 KB  
Article
Distinguishing the Mechanisms Driving Community Structure Across Different Growth Stages in Quercus Forests
by Zhenghua Lian, Yingshan Jin, Xuefan Hu, Yanhong Liu, Fang Li, Fang Liang, Yuerong Wang, Zuzheng Li, Jiahui Wang and Hongfei Chen
Forests 2025, 16(8), 1332; https://doi.org/10.3390/f16081332 - 16 Aug 2025
Viewed by 617
Abstract
Understanding the mechanisms governing forest community assembly across different growth stages is essential for revealing succession dynamics and guiding forest restoration. While much attention has been given to overstory trees, the understory regeneration layer, critical for forest succession, remains less explored, particularly regarding [...] Read more.
Understanding the mechanisms governing forest community assembly across different growth stages is essential for revealing succession dynamics and guiding forest restoration. While much attention has been given to overstory trees, the understory regeneration layer, critical for forest succession, remains less explored, particularly regarding its stage-specific survival strategies and assembly processes. This study investigates the natural regeneration of Quercus variabilis forests in northern China, focusing on the transition from early to later growth stages. Our objectives were to (1) identify the phylogenetic and functional structures of regeneration communities at early and later stages, (2) explore their responses to environmental gradients, and (3) assess the roles of deterministic and stochastic processes in shaping community assembly. We integrated phylogenetic structure, functional traits, and environmental gradients to examine natural regeneration communities. The results revealed clear stage-dependent patterns: communities exhibited random phylogenetic and functional structures in the early growth stage, suggesting a dominant role of stochastic processes during early recruitment. In contrast, communities showed phylogenetic clustering and functional overdispersion in later growth stages, indicating the increasing influence of environmental filtering and interspecific competition as individuals developed. Generalized Dissimilarity Modeling (GDM) further revealed that dispersal limitation and pH were key predictors of phylogenetic β-diversity in the later growth stage, while total phosphorus drove functional β-diversity in the later growth stage. No significant predictors were found for β-diversity in the early stage. These findings highlight the shift from stochastic to deterministic processes during forest regeneration, emphasizing the stage-dependent nature of assembly mechanisms. Our study elucidates the stage-specific assembly rules of Q. variabilis forests and offers theoretical guidance for stage-targeted interventions in forest management to promote positive succession. Full article
(This article belongs to the Special Issue Suitable Ecological Management of Forest Dynamics)
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21 pages, 2896 KB  
Article
Explainable CNN–Radiomics Fusion and Ensemble Learning for Multimodal Lesion Classification in Dental Radiographs
by Zuhal Can and Emre Aydin
Diagnostics 2025, 15(16), 1997; https://doi.org/10.3390/diagnostics15161997 - 9 Aug 2025
Viewed by 882
Abstract
Background/Objectives: Clinicians routinely rely on periapical radiographs to identify root-end disease, but interpretation errors and inconsistent readings compromise diagnostic accuracy. We, therefore, developed an explainable, multimodal AI framework that (i) fuses two data modalities, deep CNN embeddings and radiomic texture descriptors that [...] Read more.
Background/Objectives: Clinicians routinely rely on periapical radiographs to identify root-end disease, but interpretation errors and inconsistent readings compromise diagnostic accuracy. We, therefore, developed an explainable, multimodal AI framework that (i) fuses two data modalities, deep CNN embeddings and radiomic texture descriptors that are extracted only from lesion-relevant pixels selected by Grad-CAM, and (ii) makes every prediction transparent through dual-layer explainability (pixel-level Grad-CAM heatmaps + feature-level SHAP values). Methods: A dataset of 2285 periapical radiographs was processed using six CNN architectures (EfficientNet-B1/B4/V2M/V2S, ResNet-50, Xception). For each image, a Grad-CAM heatmap generated from the penultimate layer of the CNN was thresholded to create a binary mask that delineated the region most responsible for the network’s decision. Radiomic features (first-order, GLCM, GLRLM, GLDM, NGTDM, and shape2D) were then computed only within that mask, ensuring that handcrafted descriptors and learned embeddings referred to the same anatomic focus. The two feature streams were concatenated, optionally reduced by principal component analysis or SelectKBest, and fed to random forest or XGBoost classifiers; five-view test-time augmentation (TTA) was applied at inference. Pixel-level interpretability was provided by the original Grad-CAM, while SHAP quantified the contribution of each radiomic and deep feature to the final vote. Results: Raw CNNs achieved a ca. 52% accuracy and AUC values near 0.60. The multimodal fusion raised performance dramatically; the Xception + radiomics + random forest model achieved a 95.4% accuracy and an AUC of 0.9867, and adding TTA increased these to 96.3% and 0.9917, respectively. The top ensemble, Xception and EfficientNet-V2S fusion vectors classified with XGBoost under five-view TTA, reached a 97.16% accuracy and an AUC of 0.9914, with false-positive and false-negative rates of 4.6% and 0.9%, respectively. Grad-CAM heatmaps consistently highlighted periapical regions, while SHAP plots revealed that radiomic texture heterogeneity and high-level CNN features jointly contributed to correct classifications. Conclusions: By tightly integrating CNN embeddings, mask-targeted radiomics, and a two-tiered explainability stack (Grad-CAM + SHAP), the proposed system delivers state-of-the-art lesion detection and a transparent technique, addressing both accuracy and trust. Full article
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21 pages, 3251 KB  
Article
A Novel Amphibious Terrestrial–Aerial UAV Based on Separation Cage Structure for Search and Rescue Missions
by Changhao Jia, Yiyuan Xing, Zhijie Li and Xiankun Ge
Appl. Sci. 2025, 15(16), 8792; https://doi.org/10.3390/app15168792 - 8 Aug 2025
Viewed by 750
Abstract
In response to the challenges faced by unmanned aerial vehicles (UAV) in cluttered environments such as forests, ruins, and pipelines, this study introduces a ground–air amphibious UAV specifically designed for personnel search and rescue in complex environments. By innovatively designing and applying a [...] Read more.
In response to the challenges faced by unmanned aerial vehicles (UAV) in cluttered environments such as forests, ruins, and pipelines, this study introduces a ground–air amphibious UAV specifically designed for personnel search and rescue in complex environments. By innovatively designing and applying a separation cage structure, the UAV’s capabilities for ground movement and aerial flight have been enhanced, effectively overcoming the limitations of traditional single-mode robots operating in narrow or obstacle-dense areas. This design addresses the occlusion issue of sensing components in traditional caged UAVs while maintaining protection for both the UAV itself and the surrounding environment. Additionally, through the innovative design of an H-shaped quadcopter frame skeleton structure, the UAV has gained the ability to perform steady-state aerial flight while also better adapting to the separation cage structure, achieving a reduced energy consumption and significantly improving its operational capabilities in complex environments. The experimental results demonstrate that the UAV prototype, weighing 1.2 kg with a 1 kg payload capacity, achieves a 40 min maximum endurance under full payload conditions at the endurance speed of 10 m/s while performing real-time object detection. The system reliably executes multimodal operations, including stable takeoff, landing, aerial hovering, directional maneuvering, and terrestrial locomotion with coordinated steering control. Full article
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25 pages, 2136 KB  
Article
Decoding China’s Smart Forestry Policies: A Multi-Level Evaluation via LDA and PMC-TE Index
by Yafang Zhang, Yue Ren, Jiaqi Liu and Yukun Cao
Forests 2025, 16(8), 1297; https://doi.org/10.3390/f16081297 - 8 Aug 2025
Viewed by 713
Abstract
Smart forestry is gaining global prominence as countries seek to modernize forest governance through digital technologies and data-driven approaches. In China, smart forestry serves as a central pillar of ecological modernization, with policy playing a pivotal role in shaping its development. This study [...] Read more.
Smart forestry is gaining global prominence as countries seek to modernize forest governance through digital technologies and data-driven approaches. In China, smart forestry serves as a central pillar of ecological modernization, with policy playing a pivotal role in shaping its development. This study addresses these gaps by proposing an integrated evaluation framework combining thematic modeling via Latent Dirichlet Allocation (LDA) and structural assessment using the Policy Modeling Consistency–Text Encoder (PMC-TE) index. A total of 82 national and provincial policy documents (2009–2025) were analyzed to identify 13 core topics and categorize instruments into supply-side, demand-side, and environmental types. To assess structural coherence, a PMC-TE index was constructed based on a nine-variable, 32-indicator framework, with results visualized through three-dimensional PMC surfaces. Structural evaluation based on the PMC-TE index indicates that while most policies fall within the “good” or “excellent” range, notable gaps remain between policy objectives and the instruments employed to achieve them. Beyond China, the proposed framework provides a replicable tool for evaluating smart forestry governance in other countries undergoing digital transitions. The findings further highlight the need to enhance demand-side participation, strengthen closed-loop governance mechanisms, and promote cross-sectoral coordination to achieve greater policy coherence. Full article
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24 pages, 32703 KB  
Article
Spatiotemporal Evolution of Carbon Storage and Driving Factors in Major Sugarcane-Producing Regions of Guangxi, China
by Jianing Ma, Jun Wen, Shirui Du, Chuanmin Yan and Chuntian Pan
Agronomy 2025, 15(8), 1817; https://doi.org/10.3390/agronomy15081817 - 27 Jul 2025
Viewed by 519
Abstract
Objectives: The major sugarcane-producing regions of Guangxi represent a critical agricultural zone in China. Investigating the mechanisms of land use change and carbon storage dynamics in this area is essential for optimizing regional ecological security and promoting sustainable development. Methods: Employing the land [...] Read more.
Objectives: The major sugarcane-producing regions of Guangxi represent a critical agricultural zone in China. Investigating the mechanisms of land use change and carbon storage dynamics in this area is essential for optimizing regional ecological security and promoting sustainable development. Methods: Employing the land use transfer matrix, the InVEST model and the Geodetector model to analyze carbon storage changes and identify key driving factors and their interactive effects. Results: (1) From 2011 to 2022, Guangxi’s major sugarcane-producing regions experienced significant land use changes: reductions in cultivated land, grassland and water bodies alongside expansions of forest, bare land and construction land. (2) The total carbon storage in Guangxi’s major sugarcane-producing regions has increased from 2011 to 2018 by 0.99%, representing 1627.03 and 1643.10 million tons, while it has decreased by 0.1% in 2022 (1641.47 million tons) compared to 2018. (3) Cultivated land proportion and forest coverage rate were the primary drivers of spatial heterogeneity, followed by average slope and land urbanization rate. (4) Interaction analysis revealed strong synergistic effects among cultivated land proportion, forest coverage rate, NDVI and average slope, confirming multi-factor control over carbon storage changes. Conclusions: Carbon storage in the Guangxi sugarcane-producing regions is shaped by land use patterns and multi-factor interactions. Future strategies should optimize land use structures and balance urbanization with ecological protection to enhance regional carbon sequestration. Full article
(This article belongs to the Section Agroecology Innovation: Achieving System Resilience)
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22 pages, 6378 KB  
Article
Cross-Modal Insights into Urban Green Spaces Preferences
by Jiayi Yan, Fan Zhang and Bing Qiu
Buildings 2025, 15(14), 2563; https://doi.org/10.3390/buildings15142563 - 20 Jul 2025
Viewed by 652
Abstract
Urban green spaces (UGSs) and forests play a vital role in shaping sustainable and livable cities, offering not only ecological benefits but also spaces that are essential for human well-being, social interactions, and everyday life. Understanding the landscape features that resonate most with [...] Read more.
Urban green spaces (UGSs) and forests play a vital role in shaping sustainable and livable cities, offering not only ecological benefits but also spaces that are essential for human well-being, social interactions, and everyday life. Understanding the landscape features that resonate most with public preferences is essential for enhancing the appeal, accessibility, and functionality of these environments. However, traditional approaches—such as surveys or single-data analyses—often lack the nuance needed to capture the complex and multisensory nature of human responses to green spaces. This study explores a cross-modal methodology that integrates natural language processing (NLP) and deep learning techniques to analyze text and image data collected from public reviews of 19 urban parks in Nanjing. By capturing both subjective emotional expressions and objective visual impressions, this study reveals a consistent public preference for natural landscapes, particularly those featuring evergreen trees, shrubs, and floral elements. Text-based data reflect users’ lived experiences and nuanced perceptions, while image data offers insights into visual appeal and spatial composition. By bridging human-centered insights with data-driven analysis, this research provides a robust framework for evaluating landscape preferences. It also underscores the importance of designing green spaces that are not only ecologically sound but also emotionally resonant and socially inclusive. The findings offer valuable guidance for the planning, design, and adaptive management of urban green infrastructure in ways that support healthier, more responsive, and smarter urban environments. Full article
(This article belongs to the Section Architectural Design, Urban Science, and Real Estate)
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22 pages, 5529 KB  
Article
From Perception to Action: Air Pollution Awareness and Behavioral Adjustments in Pregnant Women in Serbia
by Ana Susa, Milica Zekovic, Dragana Davidovic, Katarina Paunovic, Vera Kujundzic, Sladjana Mihajlovic and Ljiljana Bogdanovic
Healthcare 2025, 13(12), 1475; https://doi.org/10.3390/healthcare13121475 - 19 Jun 2025
Viewed by 1045
Abstract
In regions with sustained air pollution, the adoption of protective health behaviors is critical, particularly among pregnant women—a population marked by physiological vulnerability and heightened receptivity to preventive guidance. Understanding and supporting patient-driven behavioral change requires attention to individual perception and awareness, which [...] Read more.
In regions with sustained air pollution, the adoption of protective health behaviors is critical, particularly among pregnant women—a population marked by physiological vulnerability and heightened receptivity to preventive guidance. Understanding and supporting patient-driven behavioral change requires attention to individual perception and awareness, which are shaped by socio-economic and spatial factors, as well as access to credible information. Objectives: This study investigates how pregnant women in Serbia perceive air quality, identifies determinants that influence these perceptions, and evaluates the extent and nature of behavioral adaptations undertaken to mitigate exposure-related risks. Methods: A cross-sectional survey was conducted among 279 pregnant women using a structured, researcher-administered questionnaire. Collected data included demographic and psychosocial variables, air quality perceptions, self-reported health effects, and behavioral responses. Residential proximity to land-use attributes was assessed using GIS-based spatial analysis. Results: Most participants perceived air quality as poor (68.8%), primarily informed by unofficial sources such as mobile applications and social media. Living close to continuous urban fabric (OR = 0.180, 95% CI: 0.059–0.558, p = 0.003) and water (OR = 0.306, 95% CI: 0.127–0.738, p = 0.008) was associated with poorer perceptions, while proximity to forests (OR = 2.938, 95% CI: 1.323–6.525, p = 0.008) correlated with more favorable assessments. Despite prevalent concern, around half of respondents (50.2%) reported no behavioral modifications. Importantly, none had received guidance from healthcare professionals on the topic. Conclusions: These findings highlight critical gaps in environmental health literacy and provider engagement. Integrating tailored communication and behavioral support in existing prenatal counseling could advance health-related quality of life in this vulnerable population. Full article
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24 pages, 4557 KB  
Article
Advanced Multi-Level Ensemble Learning Approaches for Comprehensive Sperm Morphology Assessment
by Abdulsamet Aktas, Taha Cap, Gorkem Serbes, Hamza Osman Ilhan and Hakkı Uzun
Diagnostics 2025, 15(12), 1564; https://doi.org/10.3390/diagnostics15121564 - 19 Jun 2025
Cited by 3 | Viewed by 889
Abstract
Introduction: Fertility is fundamental to human well-being, significantly impacting both individual lives and societal development. In particular, sperm morphology—referring to the shape, size, and structural integrity of sperm cells—is a key indicator in diagnosing male infertility and selecting viable sperm in assisted reproductive [...] Read more.
Introduction: Fertility is fundamental to human well-being, significantly impacting both individual lives and societal development. In particular, sperm morphology—referring to the shape, size, and structural integrity of sperm cells—is a key indicator in diagnosing male infertility and selecting viable sperm in assisted reproductive technologies such as in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI). However, traditional manual evaluation methods are highly subjective and inconsistent, creating a need for standardized, automated systems. Objectives: This study aims to develop a robust and fully automated sperm morphology classification framework capable of accurately identifying a wide range of morphological abnormalities, thereby minimizing observer variability and improving diagnostic support in reproductive healthcare. Methods: We propose a novel ensemble-based classification approach that combines convolutional neural network (CNN)-derived features using both feature-level and decision-level fusion techniques. Features extracted from multiple EfficientNetV2 variants are fused and classified using Support Vector Machines (SVM), Random Forest (RF), and Multi-Layer Perceptron with Attention (MLP-Attention). Decision-level fusion is achieved via soft voting to enhance robustness and accuracy. Results: The proposed ensemble framework was evaluated using the Hi-LabSpermMorpho dataset, which contains 18 distinct sperm morphology classes. The fusion-based model achieved an accuracy of 67.70%, significantly outperforming individual classifiers. The integration of multiple CNN architectures and ensemble techniques effectively mitigated class imbalance and enhanced the generalizability of the model. Conclusions: The presented methodology demonstrates a substantial improvement over traditional and single-model approaches in automated sperm morphology classification. By leveraging ensemble learning and multi-level fusion, the model provides a reliable and scalable solution for clinical decision-making in male fertility assessment. Full article
(This article belongs to the Section Machine Learning and Artificial Intelligence in Diagnostics)
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15 pages, 1496 KB  
Article
Radiomics-Based Classification of Clear Cell Renal Cell Carcinoma ISUP Grade: A Machine Learning Approach with SHAP-Enhanced Explainability
by María Aymerich, Alejandra García-Baizán, Paolo Niccolò Franco, Mariña González, Pilar San Miguel Fraile, José Antonio Ortiz-Rey and Milagros Otero-García
Diagnostics 2025, 15(11), 1337; https://doi.org/10.3390/diagnostics15111337 - 26 May 2025
Viewed by 1254
Abstract
Background: Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cancer, and its prognosis is closely linked to the International Society of Urological Pathology (ISUP) grade. While histopathological evaluation remains the gold standard for grading, non-invasive methods, such [...] Read more.
Background: Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cancer, and its prognosis is closely linked to the International Society of Urological Pathology (ISUP) grade. While histopathological evaluation remains the gold standard for grading, non-invasive methods, such as radiomics, offer potential for automated classification. This study aims to develop a radiomics-based machine learning model for the ISUP grade classification of ccRCC using nephrographic-phase CT images, with an emphasis on model interpretability through SHAP (SHapley Additive exPlanations) values. Objective: To develop and interpret a radiomics-based machine learning model for classifying ISUP grade in clear cell renal cell carcinoma (ccRCC) using nephrographic-phase CT images. Materials and Methods: This retrospective study included 109 patients with histopathologically confirmed ccRCC. Radiomic features were extracted from the nephrographic-phase CT scans. Feature robustness was evaluated via intraclass correlation coefficient (ICC), followed by redundancy reduction using Pearson correlation and minimum Redundancy Maximum Relevance (mRMR). Logistic regression, support vector machine, and random forest classifiers were trained using 8-fold cross-validation. SHAP values were computed to assess feature contribution. Results: The logistic regression model achieved the highest classification performance, with an accuracy of 82% and an AUC of 0.86. SHAP analysis identified major axis length, busyness, and large area emphasis as the most influential features. These variables represented shape and texture information, critical for distinguishing between high and low ISUP grades. Conclusions: A radiomics-based logistic regression model using nephrographic-phase CT enables accurate, non-invasive classification of ccRCC according to ISUP grade. The use of SHAP values enhances model transparency, supporting clinical interpretability and potential adoption in precision oncology. Full article
(This article belongs to the Section Machine Learning and Artificial Intelligence in Diagnostics)
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18 pages, 2158 KB  
Article
Relationship Between Forest Structure and Soil Characteristics with Flooded and Non-Flooded Rainforests of Northern Amazonia (Brazil)
by Edyrlli Naele Barbosa Pimentel, Lucas Botelho Jerônimo, Manoel Tavares de Paula, María Vanessa Lencinas, Guillermo Martínez Pastur and Gerardo Rubio
Forests 2025, 16(5), 793; https://doi.org/10.3390/f16050793 - 9 May 2025
Cited by 1 | Viewed by 1226
Abstract
Environmental variability modifies forest structure through interactions among soil properties, topography, and climate. These factors influence the occurrence of contrasting forest types in northern Amazonia (Brazil), such as forests in highlands (Terra Firme) and forests under regular flooding (Várzea). Flooding regimes influence soil [...] Read more.
Environmental variability modifies forest structure through interactions among soil properties, topography, and climate. These factors influence the occurrence of contrasting forest types in northern Amazonia (Brazil), such as forests in highlands (Terra Firme) and forests under regular flooding (Várzea). Flooding regimes influence soil formation and modify soil geochemistry, nutrient distribution, and organic matter accumulation, shaping forest structure and composition. The objective was to determine the relationships between structure and soil characteristics in non-flooded and flooded tropical forests. We compared forest structure and soil characteristics at both conditions (n = 2 treatments × 20 replicas = 40 plots) using univariate and multivariate analyses. We found significant differences in most of the studied variables between forest types, both chemical and physical properties. Our results showed that flooding defines forest structure and composition (e.g., tree density, height, and volume) and influences soil nutrient characteristics. Floodplain forests exhibited higher soil nutrient concentration and organic carbon content, likely due to periodic litter accumulation, sediments, and reduced decomposition rates. In contrast, non-flooded forests were characterized by lower nutrient levels, higher sand content, and greater forest structure values (e.g., height, basal area, and volume). These insights contribute to understanding the functioning of both forest ecosystems. Full article
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13 pages, 833 KB  
Article
Prediction of Pituitary Adenoma’s Volumetric Response to Gamma Knife Radiosurgery Using Machine Learning-Supported MRI Radiomics
by Herwin Speckter, Marko Radulovic, Erwin Lazo, Giancarlo Hernandez, Jose Bido, Diones Rivera, Luis Suazo, Santiago Valenzuela, Peter Stoeter and Velicko Vranes
J. Clin. Med. 2025, 14(9), 2896; https://doi.org/10.3390/jcm14092896 - 23 Apr 2025
Viewed by 1123
Abstract
Background/Objectives: Gamma knife radiosurgery (GKRS) is widely performed as an adjuvant management of patients with residual or recurrent pituitary adenoma (PA). However, the variability in the tumor volume response to GKRS emphasizes the need for reliable predictors of treatment outcomes. The application of [...] Read more.
Background/Objectives: Gamma knife radiosurgery (GKRS) is widely performed as an adjuvant management of patients with residual or recurrent pituitary adenoma (PA). However, the variability in the tumor volume response to GKRS emphasizes the need for reliable predictors of treatment outcomes. The application of radiomics, an analytical approach for quantitative imaging, remains unexplored in predicting treatment responses for PAs. This study aimed to pioneer the use of radiomic MRI analysis to predict the volumetric response of PA to GKRS. Methods: This retrospective observational cohort study involved 81 patients who underwent GKRS for PA. Pre-treatment 3-Tesla MRI scans were used to extract radiomic features capturing the intensity, shape, and texture of the tumors. Radiomic signatures were generated using the least absolute shrinkage and selection operator (LASSO) for feature selection, in conjunction with several classifiers: random forest, naïve Bayes, kNN, logistic regression, neural network, and SVM. Results: The models demonstrated predictive performance in the test folds, with AUC values ranging from 0.759 to 0.928 and R2 values between 0.272 and 0.665. Single-sequence T1w, dual-sequence T1w + CE-T1w, and multi-modality including clinicopathological (CP) parameters (CP + T1w + CE-T1w) achieved rather similar prognostic performance in the test folds, with respective AUCs of 0.928, 0.899, and 0.909. All these radiomics models significantly outperformed a benchmark model involving only CP features (AUC = 0.846). Conclusions: This study represents a radiomic analysis focused on predicting the volume response of PAs to GKRS to facilitate treatment individualization. The developed MRI-based radiomics models exhibited superior classification performance compared with the benchmark model composed solely of standard clinicopathological parameters. Full article
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