Search Results (425)

Background: Sarcopenia and metabolic deterioration are major health concerns in adults aged ≥ 75 years with type 2 diabetes (T2DM), a population characterized by anabolic resistance, reduced dietary intake, and limited renal reserve. Optimizing protein nutrition may support muscle maintenance in this high-risk group, but clinical evidence for individualized high-protein guidance in the oldest-old population remains limited. Objective: We investigated whether an 18-month dietary intervention improves muscle mass and strength in adults aged ≥ 75 years with T2DM and whether serum amino acid (AA) and hormonal profiles reflect these changes. Methods: In this 18-month, single-arm, prospective intervention study, 44 community-dwelling adults aged ≥ 75 years with T2DM received individualized, dietitian-led nutritional guidance targeting a protein intake of approximately 1.4 g/kg ideal body weight (IBW)/day. Assessments at baseline and every 6 months included body composition, muscle strength, renal function, and fasting serum amino acid and hormonal profiles. Longitudinal changes were analyzed using paired t-tests and linear mixed-effects models. This trial was registered in the UMIN Clinical Trials Registry (UMIN000044687). Results: Skeletal muscle index and grip strength showed significant improvements at specific time points during follow-up (both p < 0.05), while gait speed improved at 6 months. Renal function remained clinically stable (eGFRcreat slope: +0.18 mL/min/1.73 m²/year; eGFRcys slope: −2.97 mL/min/1.73 m²/year), with no significant increase in CKD stage. Changes in glucagon correlated positively and C-peptide negatively with changes in skeletal muscle index, whereas glucagon was inversely associated with grip strength. Serum fibroblast growth factor 21 (FGF21) levels decreased over time, suggesting metabolic adaptation to the intervention. Conclusions: Individualized high-protein nutritional guidance for 18 months improved sarcopenia-related parameters, including skeletal muscle index and grip strength, without clinically significant deterioration of renal function in adults aged ≥ 75 years with T2DM. These findings support the feasibility and safety of protein-focused dietary counseling as a strategy to preserve muscle health in advanced age. Full article

Biathlon is a winter Olympic sport that combines high-intensity cross-country skiing with precise rifle shooting. These dual demands require athletes to develop exceptional aerobic capacity while maintaining fine motor accuracy under physiological stress. Despite its complexity, nutritional strategies in biathlon remain under-explored and recommendations are often extrapolated from related endurance sports. This narrative review aims to summarise existing knowledge on nutrition in biathlon, highlight sport-specific challenges and identify areas for future research. The main findings indicate that biathletes face very high energy demands, with daily expenditure exceeding 7000 kcal during intensive training. Carbohydrates are the primary fuel source, with intake recommendations based on training intensity and duration (6–12 g/kg/d). Furthermore, protein is essential for muscle repair, recovery and adaptation. To achieve the recommended intake of 1.6 g/kg/day, it is advisable to consume meals containing approximately 0.3 g/kg of high-quality protein every three to four hours. Given the frequency of training sessions, effective recovery strategies are important in biathlon. When recovery is a priority, biathletes should consume a meal comprising protein (approximately 0.3 g/kg) and carbohydrates (approximately 1.2 g/kg) before key training sessions. Micronutrient and vitamin deficiencies are not commonly observed in biathletes due to their high calorie intake. However, concerns regarding iron and vitamin D are common among endurance athletes due to the high risk of low energy intake, diets lacking in iron, and insufficient exposure to sunlight. On the day of the race, it is recommended that biathletes plan their meals to ensure that they meet their nutritional needs and begin recovery as soon as possible after the race is over. Biathletes may use specific supplements to enhance performance and health during preparation and competition. However, it is important to note that some supplements that improve performance may harm shooting accuracy. Current guidance is provisional, and future research should adopt a dual-performance framework that evaluates both endurance output and shooting precision under realistic competition conditions. Full article

Plant-based meat and sausage analogues (PBMAs) are gaining popularity due to growing concerns about the health, environmental, and ethical impacts of animal-based foods. In the present study, we compared the nutritional quality of 298 PBMAs to 294 animal-based reference products available on the German retail market in 2024 across nine subcategories. PBMAs generally contained less total fat, saturated fat, and protein, but more fibre, carbohydrates, and sugar compared to meat products. Notably, most meat analogues exhibited higher salt contents. Nutri-Scores of PBMAs were significantly favourable in six subcategories. Among PBMAs, approximately 60% demonstrated good protein quality, while only 12% were fortified with key micronutrients, including vitamin B12 or iron. Most PBMAs contained one or more additives and flavourings. These findings suggest that while PBMAs often offer favourable nutritional profiles, reformulation and clearer nutritional guidance could help to further support the emergence of healthier plant-based options. Full article

Background: Plant-based diets have been widely recommended as sustainable, ethical, and health-conscious alternatives. However, their adoption remains limited due to nutritional and cultural concerns, which may be reinforced in contexts associated with masculinity. This study aimed to explore (a) whether physical activity represents a barrier to plant-based food choices, and (b) whether beliefs about plant-based diets differ between sedentary and physically active individuals. Methods: A mixed-methods study was conducted, combining focus groups and survey data. Twenty-six Italian volunteers were divided into four groups: (I) physically active omnivores; (II) sedentary omnivores; (III) physically active veg*ans; and (IV) sedentary veg*ans. The discussions focused on protein-rich foods (animal-based vs. plant-based) and addressed nutritional concerns related to athletic performance, as well as attitudes toward plant-based proteins and veg*an individuals. Results: Omnivores—particularly active ones—favored animal-based proteins, perceiving them as more palatable, nutritious, and better suited for physical performance, a view often reinforced by coaches. This group also emphasized sensory pleasure as a barrier to adopting plant-based alternatives. It addressed masculinity-related themes more frequently; moreover, while veg*an participants were more inclined to seek professional advice to ensure a balanced diet, omnivores referred to the traditional Mediterranean diet as their primary source of nutritional guidance. This factor may further hinder openness to plant-based choices. Conclusions: The findings highlight stronger stigma among physically active individuals and suggest the need for interventions that replace the current narrative of plant-based diets as a restrictive or dietetic alternative with one that frames it as compatible with strength, vitality, and overall well-being. Full article

Precision Feeding Systems (PFS) demonstrate transformative potential in advancing sustainable and efficient production within modern animal husbandry. However, existing research lacks a synthesis of PFS applications in livestock farming and offers little targeted guidance for China’s rapidly growing rabbit industry. The objective of this review is to bridge this gap by synthesizing current knowledge on PFS technologies—including sensor networks, artificial intelligence (AI), automated controls, and data analytics—and providing a structured framework for their implementation in rabbit production. This study selects and analyzes 112 core references, establishing a foundational database for comprehensive evaluation. The key contributions of this work are threefold: first, it outlines the core components and operational mechanisms of PFS; second, it identifies major challenges such as sensor reliability in dynamic environments, data security risks, limited explainability of AI models, and interoperability barriers; and third, it proposes a customized strategy for PFS adoption in rabbit farming, emphasizing phased implementation, cross-system integration, and iterative optimization. The primary outcomes and advantages of adopting such a system include significant improvements in feed efficiency, resource utilization, animal welfare, and waste reduction—critical factors given rabbits’ sensitive digestive systems and precise nutritional needs. Furthermore, this review outlines a future research agenda aimed at developing resilient sensors, explainable AI frameworks, and multi-objective optimization engines to enhance the commercial scalability and sustainability of PFS in rabbit husbandry and beyond. Full article

Background/Objectives: This study assessed the chemical and physical stability of ascorbic acid in pediatric total parenteral nutrition (TPN) admixtures under conditions reflecting both hospital compounding and home administration. Methods: Two storage protocols were examined: (A) refrigerated storage (15 days, 4 ± 2 °C) followed by addition of ascorbic acid and a 24-h period of storage at room temperature, and (B) vitamin supplementation within 24 h after composing and storage at 21 ± 2 °C. A validated high-performance liquid chromatography (HPLC) method was used to quantify ascorbic acid degradation. Physical stability was evaluated via optical microscopy, dynamic light scattering (DLS), laser diffraction (LD), zeta potential, and pH measurement. Results: Ascorbic acid content remained above 90% of the declared value in both protocols, although gradual degradation was observed with increasing storage time and temperature. Emulsion droplet sizes remained within pharmacopeial limits (<500 nm), and no coalescence or phase separation was detected. Zeta potential values (−20 to −40 mV) confirmed kinetic stability, while pH ranged from 5.8 to 6.2, remaining within acceptable safety margins. Conclusions: Vitamin C in pediatric TPN admixtures is stable under refrigerated conditions for up to 15 days. However, the additional 24 h at room temperature resulted in measurable loss of ascorbic acid content, suggesting a need for improved guidance in home-based parenteral nutrition, particularly regarding transport and handling. The study underscores the importance of strict cold-chain maintenance and highlights the role of emulsion matrix and packaging in protecting labile vitamins. This research provides practical implications for hospital pharmacists and caregivers, supporting better formulation practices and patient safety in pediatric home TPN programs. Full article

Background: Circadian rhythm disruption caused by shift work alters metabolic and hormonal pathways, which accelerates chronic disease onset, leading to decreased quality and quantity of life. This study aimed to determine whether a practical lifestyle intervention emphasizing nutrition timing and recovery habits could mitigate the metabolic and psychological effects of night-shift work. We conducted a randomized, open-label, crossover trial with two 8-week periods. Methods: Female healthcare workers (n = 13) aged 18–50 years with a body mass index (BMI) between 27 and 40 kg/m² and working predominantly night shifts (≥30 h/week for ≥6 months) were randomized. During the 8-week intervention phase, participants received daily text messages with guidance on food, sleep/rest, and physical activity and were provided with whey protein isolate powder and grain-based snack bars to consume during work shifts. The program targeted improved nutrient timing, adequate protein intake, and structured rest without formal exercise training, allowing evaluation of dietary and behavioral effects feasible for this population. Total caloric (~30 kcal/kg lean mass) and protein (2 g/kg lean mass) needs were measured, along with sleep/rest goals of 6–8 h/24 h. Primary outcome measures were change in visceral fat percentage (VF%) by DXA and mental/physical quality of life (RAND SF-12). Secondary outcomes included fasting triglycerides, ALT, blood glucose, LDL, actigraphy, and fecal microbiome. Mixed-design two-way ANOVA was conducted to assess the effects of group (immediate [IG] and delayed [DG]), time (baseline, 8-week crossover, and week 16), and Group $\times$ Time (GxT) interactions, and Bonferroni correction was applied to post hoc t-tests. Results: Eleven participants completed the study. Both groups increased dietary protein intake (p < 0.001), and a GxT interaction for VF% (p = 0.039) indicated DG reduced VF% to a greater extent (−0.335 ± 0.114% (p = 0.003) vs. 0.279 ± 0.543% (p = 0.158)). Mental and physical QOL, objectively measured physical activity and sleep, serum lipids and inflammatory markers, and fecal microbiota remained unchanged (p > 0.05 for all GxT). Conclusions: The findings suggest that targeted nutrition and recovery strategies can modestly improve dietary intake and visceral fat; however, consistent with prior work, interventions without structured exercise may be insufficient to reverse broader metabolic effects of circadian disruption. This trial was registered at ClinicalTrials.gov, identifier: NCT06158204, first registered: 28 November 2023. Full article

Potato-based reconstituted rice represents an innovative staple food solution that addresses nutritional and economic challenges. Using fresh potatoes instead of potato flour eliminates nutrient loss and reduces energy costs associated with traditional processing methods. This study examined reconstituted rice produced from the yellow-fleshed ‘Dianshu 1428’ and purple-fleshed ‘Diancaishu 101’ potato varieties, comparing their nutritional and aromatic profiles with commercial rice-based alternatives. The results demonstrated significant nutritional advantages: potato-based reconstituted rice contains 3 g/100 g dietary fiber, a five-fold higher potassium content, and 11–12 times more iron than conventional rice-based reconstituted rice. Unique aroma compounds, including methional, 2-ethyl-3,5-dimethylpyrazine, α-ionone, and (E,E)-2,6-nonadienal, impart distinctive potato, nutty, fruity, and fatty flavors. Yellow-fleshed varieties contributed 14.1 μg/100 g carotenoids, while purple-fleshed varieties provided 45 mg/100 g anthocyanins. These findings establish that potato-based reconstituted rice offers a superior nutritional composition and unique sensory characteristics compared to traditional alternatives, providing scientific guidance for the selection of the potato variety and product optimization in developing nutritionally enhanced potato staple foods with specific functional and sensory attributes. Full article

Nutrition is a cornerstone of public health, yet the unique nutrition needs and considerations of lesbian, gay, bisexual, transgender, and others (LGBT+) communities remain largely invisible in the field of dietetics. These populations face disproportionate burdens of obesity, eating disorders, body dysmorphia, metabolic risks, and food insecurity, often driven by stigma, minority stress, and structural inequities. This narrative review aimed to synthesize current evidence on nutrition-related disparities among LGBT+ populations and identify opportunities for dietitians to advance equity in care. A comprehensive search of PubMed, Scopus, and Web of Science was conducted for studies addressing diet quality, obesity, eating disorders, food insecurity, and metabolic health in sexual and gender minorities. Evidence indicates clear subgroup differences: lesbian and bisexual women are more likely to experience obesity and food insecurity; gay and bisexual men report lower BMI but greater body image concerns and disordered eating; transgender individuals face nutritional challenges linked to gender-affirming therapy and high rates of food insecurity; and people living with HIV encounter additional metabolic risks associated with treatment. Despite these findings, LGBT+ health remains rarely reflected in dietary guidelines or professional training. Embedding inclusivity into dietetic education and clinical encounters, adopting culturally competent and structurally aware practices, and tailoring interventions to subgroup-specific needs are key priorities. Inclusive, equity-driven, and person-centered nutrition care is essential to closing health gaps for LGBT+ populations and ensuring that every patient receives guidance that affirms their identity and lived experience. Full article

Oyster mushrooms (Pleurotus spp.) are widely cultivated due to their high nutritional value and bioactive compounds with health-promoting properties. However, the fruiting body developmental stage significantly influences the centesimal composition and bioactive compound levels. This study examined the centesimal composition and bioactive properties of five commercial oyster mushroom species (P. citrinopileatus, P. cornucopiae, P. djamor, P. ostreatus, and P. pulmonarius) cultivated in northern Thailand at three maturation stages (young, middle, and mature). The centesimal composition; polysaccharide, ergothioneine, and phenolic compound contents; antioxidant activity; and α-glucosidase inhibitory activity were analyzed. The results showed that the centesimal composition and polysaccharide content increased as the mushrooms matured in all species. The middle stage consistently exhibited the highest levels of ergothioneine, total phenolics, and individual phenolic compounds in all five species. Twelve phenolic compounds were identified, with 4-hydroxybenzoic acid, trans-cinnamic acid, and trans-o-coumaric acid being predominant. All extracts exhibited antioxidant activity, according to the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays, and α-glucosidase inhibitory activity, with the highest activity found at the middle stage. This is the first paper to report the ergothioneine content and α-glucosidase inhibitory activity in P. cornucopiae and P. djamor. These findings demonstrate that harvest timing can be optimized to maximize either the nutritional content (mature stage) or bioactive compound content for functional food applications (middle stage), offering a better understanding of the developmental phases at which mushrooms have the greatest health and technological potential. Furthermore, this knowledge provides practical guidance for growers seeking to target specific markets for high nutritional value foods based on consumer demand and for functional food developers aiming to maximize health-promoting properties. Full article

Objectives: Few studies have examined maternal dietary intakes in relation to children’s malnutrition status. We examined variations in children stunting status and their association with maternal and child dietary intakes. Methods: This one-year prospective study (conducted from November 2021 to December 2022) consisted of up to four surveys carried out in rural Kenya. It included 135 pairs of children aged 12–59 months and their non-pregnant mothers, all of whom had received nutrition guidance during the study. Dietary intakes were assessed in four non-consecutive 24 h dietary recalls during the first two surveys. Anthropometric measurements were taken at most four times, and variations in children stunting status (not-stunted, recovered-from-stunting, or persistent/worsened stunting) were assessed. Maternal and child dietary intakes, based on variations in stunting status, were compared using one-way analysis of covariance adjusted for socio-demographic variables. Results: Of the 135 children studied, 40 (29.6%) were stunted at baseline, whereas 85, 20, and 30 had no stunting, recovered from stunting, or had persistent/worsened stunting. Children with persistent/worsened stunting had a significantly lower energy intake than other children; however, maternal energy intake did not differ by children’s stunting status. Milk intake was significantly lower among children with persistent/worsening stunting than other children. A similar difference based on variations in stunting was also observed for maternal milk intake. Conclusions for Practice: The mothers of rural Kenyan children who had recovered from stunting consumed the most milk, while the mothers of children with persistent/worsening consumed the least milk. Further research is needed to confirm the factors behind the observed intake differences. Full article

Background: The rapid aging of China’s population poses significant challenges, particularly in public health and medical services. Frailty, a reversible geriatric syndrome, is a critical intervention target for disability prevention among older adults. Objective: We hypothesize that both intervention groups will demonstrate significant improvements in Short Physical Performance Battery (SPPB) scores compared to the control group, and that these improvements will be accompanied by parallel reductions in inflammatory markers and beneficial alterations in the gut microbiota. Methods: The MultIfaceted NUtrition inTervention for frail Elderly (MINUTE) trial is a randomized, parallel-group controlled trial. In Beijing, China, 315 frail older adults were recruited and randomly assigned to 3 groups: a control group receiving routine community health management only, multifaceted nutrition intervention group, and a multifaceted nutrition and exercise combined intervention group, each comprising 105 participants. The study consists of a three-month intervention period followed by a nine-month follow-up. During the three-month intervention period, the control group receives routine community health management, while the multifaceted nutrition intervention group receives daily dietary guidance, personalized nutrition consultations, and health education. Additionally, the combined intervention group receives exercise interventions in addition to the nutritional components. After the three-month intervention, all three groups will be followed up for nine months to assess the sustainability of the study. Results: The primary outcomes are the changes in the SPPB scores. The secondary outcomes include frailty scores, intrinsic capacity, malnutrition risk, frailty recovery rates, serum differential metabolites, inflammatory factors, and gut microbiota changes. This study aims to establish a scalable and sustainable pathway for frailty prevention among community-dwelling older adults in China and provide valuable insights to inform strategies for healthy aging. Trial registration: This study is conducted in accordance with the Declaration of Helsinki and approved by the Peking University Institutional Review Board (IRB00001052-23178) on February 3, 2024, with all amendments subject to prior review and approval. Informed consent is obtained from participants, and findings will be disseminated through peer-reviewed publications, conference presentations, and summaries for school staff and participants. ClinicalTrials.gov (NCT06547593) registered 30 July 2024. Full article

Protein supplements are popular nutritional supplements consumed primarily by physically active individuals with increased protein demands. Despite the increasing consumer demand for protein supplements in Greece, detailed and comparative data on the nutritional profile of such products is scarce. The purpose of this study was to determine the nutritional quality of protein powder supplements available in the Greek market and to compare animal- with plant-based products. Data was extracted from the websites of the major retailers (n = 28). In total, 216 products were identified and grouped as animal- and plant-based, depending on the protein origin. Animal-based products were predominantly (84.0%) produced from whey. Protein content was significantly higher (p < 0.05) in animal-based products, providing 43.5% of the reference intake (RI) for men and 53.2% for women per serving. The content of essential amino acids (EAAs), branched amino acids (BCAAs) and alanine (Ala) was significantly higher (p < 0.05) in animal-based products (median: 11.0, 5.3 and 1.2 g/serving) compared with plant-based alternatives (median: 8.4, 4.0 and 1.0 g/serving size). Plant-based protein supplements contained significantly higher (p < 0.05) content of fiber, fat and salt and were more energy-dense per 100 g. Mean serving size was larger for animal-based products (29.9 ± 0.4 g) compared with the plant-based ones (28.1 ± 0.5 g). Animal-based supplements were more expensive to purchase by 4.3 € per kg. Overall, animal-based protein powder supplements show a more desirable nutritional profile regarding protein content and quality. Results of this study can serve as a tool for consumers to make informed and healthy choices and for health professionals to provide effective and personalized guidance based on the nutritional content of products. Full article

Laying hens in the late laying period often experience reduced productivity and declining egg and meat quality, which limits breeding efficiency and resource utilization. This study aimed to evaluate the effects of multi-component Botanical Crude Extracts (BCEs) on egg and meat quality, metabolic health, and gut microbiota in aged laying hens. A total of 4320 hens were supplemented with 0.3% BCEs for 100 days, with evaluations at 60 and 100 days. BCE supplementation significantly enhanced egg flavor by promoting aromatic and fat-soluble volatiles and reducing odorous compounds (p < 0.05). BCEs improved yolk nutrition by enriching n-3 polyunsaturated fatty acids, especially docosahexaenoic acid (DHA), and optimizing the n-6/n-3 ratio (p < 0.05). A moderate reduction in amino acids was observed, which may reduce bitterness and ammonia burden (0.05 ≤ p < 0.10, trend). In muscle, BCEs improved protein–fat distribution, increased intramuscular fat, and enhanced flavor-related metabolites, significantly improving meat quality of culled hens (p < 0.05). BCEs also reshaped gut microbiota, reducing harmful taxa and promoting short-chain fatty acid and aromatic metabolite biosynthesis (p < 0.05). Serum metabolomics revealed modulation of AMPK, calcium, and cholesterol pathways, improving antioxidant capacity and lipid regulation (p < 0.05). Correlation analyses linked beneficial bacteria and metabolites with yolk DHA levels and flavor (p < 0.05). Overall, BCEs enhanced egg and meat quality and physiological health, providing guidance for functional feed strategies in aged laying hens. Full article

Background: The Healthy Japan 21-Phase III dietary recommendations comprise a staple food, main dish, and side dish to maintain nutritional balance and support healthy child growth. The relationship between the frequency of such balanced meals and early adiposity rebound (AR), a predictor of obesity, remains unclear. Objective: This study aimed to examine the association between the frequency of balanced meals (staple food, main dish, and side dish) and early AR in preschool children. Methods: In this cross-sectional secondary analysis of nationwide online survey data of 688 mothers of children aged 3–6 years, dietary habits were assessed using a validated NutriSTEP-based 22-item Japanese Nutrition Screening Questionnaire. AR constituted a body mass index (BMI) increase from the 18- to 36-month health checkups recorded in the Maternal and Child Health Handbook. Risk scores reflecting lower frequency of balanced meals were calculated for staple foods, main dishes, and side dishes. Logistic regression evaluated associations between dietary risk scores and AR, adjusting for the child’s sex, age, gestational age, birth weight, daycare attendance, and parental obesity. Results: Among 688 children, 193 (28.1%) exhibited early AR and had significantly higher BMI at age 3 and the most recent measurement (both p < 0.01). A higher total dietary risk score was independently associated with AR (adjusted odds ratio; 2.58 [95% CI: 1.08–6.16]). In addition, the absolute risk difference between high- and low-risk groups was 8.5% (95% CI: 1.7–15.2%). Conclusions: A lower frequency of balanced meals is associated with early AR. These findings suggest that a simple, meal-balance screening tool could potentially aid in the early identification of the risk of later obesity and timely nutritional guidance. Full article