Search Results (3,273)

Plum peel is a major by-product of plum processing and a rich source of nutrients and bioactive compounds. This study aimed to optimize a black plum peel spread formulated with apple puree (20–40%) and plum puree (10–30%) using response surface methodology (RSM). Increasing apple puree up to 30% reduced acidity, firmness, cohesiveness, and consistency while improving sourness and overall acceptability. At 40%, apple puree decreased total acceptability and sourness while slightly increasing texture parameters. Increasing plum puree up to 20% lowered acidity, firmness, consistency, cohesiveness, viscosity, and sourness, but further increases to 30% reversed these effects. Both apple and plum purees enhanced antioxidant capacity in a concentration-dependent manner. The interaction between apple and plum purees notably affected the viscosity of the spread. Overall, plum puree had the strongest influence on texture and color, while apple puree primarily affected sensory acceptance. The optimal formulation was 32.01% apple puree and 28.16% plum puree (R² = 0.999). Developing a spread from black plum peel demonstrates a sustainable strategy for upcycling fruit-processing waste into nutritious, high-value products. This approach reduces environmental impact, supports circular food production, and creates new opportunities for functional spreads in the health-oriented food markets. Full article

Background: Reserve Officer Training Corps (ROTC) Cadets undergo biannual Field Training Exercises (FTX) that impose substantial physiological demands, necessitating adequate nutritional intake to support performance and recovery. Methods: Energy Expenditure (EE) measured by actigraphy and self-reported nutritional intake (NI) of ROTC Cadets during a Fall FTX were obtained and compared to Military Dietary Reference Intake (MDRI) guidelines. Energy balance and nutrient adequacy were assessed using paired sample t-tests. Results: Cadets demonstrated significant caloric deficits, consuming fewer kilocalories than both their active metabolic rate (t = −12.07, df = 42, p < 0.001) and Low Energy Availability thresholds (t = 6.47, df = 57.54, p < 0.001). Macronutrient analysis revealed widespread deficiencies. Neither male nor female cadets met minimum carbohydrate gram recommendations. Protein intake in grams was significantly below MDRI guidelines for 94% of males (t = −10.03, p < 0.001) and 90% of females (t = −4.62, p = 0.001). Fat intake was generally adequate for all cadets, with 94% of males (t = 6.50, p < 0.001) and 90% of females (t = 4.19, p = 0.002) meeting or exceeding recommended fat intake. Conclusions: These findings underscore the prevalence of under-fueling during FTX and highlight the need for improved nutritional strategies to mitigate energy deficits and support cadet performance and health. Full article

The increasing challenges posed by climate change demand holistic approaches to mitigate ecosystem degradation. In Mediterranean-type regions—biodiversity hotspots facing intensified droughts, fires, and biological invasions—such strategies are particularly relevant. Among invasive species, Acacia longifolia produces substantial woody and leafy biomass when removed, offering an opportunity for reuse as soil-improving material after adequate processing. This study aimed to evaluate the potential of invasive A. longifolia Green-waste compost (Gwc) as a soil amendment to promote soil recovery and native plant establishment after fire. A field experiment was carried out in a Mediterranean ecosystem using Arbutus unedo, Pinus pinea, and Quercus suber planted in control and soils treated with Gwc. Rhizospheric soils were sampled one year after plantation, in Spring and Autumn, to assess physicochemical parameters and microbial community composition (using composite samples) through Next-Generation Sequencing. Our study showed that Gwc-treated soils exhibited higher moisture content and nutrient availability, which translated into improved plant growth and increased microbial richness and diversity when compared with control soils. Together, these results demonstrate that A. longifolia Gwc enhances soil quality, supports increased plant fitness, and promotes a more diverse microbiome, ultimately contributing to faster ecosystem recovery. Transforming invasive biomass into a valuable resource could offer a sustainable, win–win solution for ecological rehabilitation in fire-affected Mediterranean environments, enhancing soil and ecosystem functioning. Full article

As an important natural enemy insect, Harmonia axyridis has significant biological control effects on a variety of pests such as aphids in agroforestry ecosystems. The large-scale application of H. axyridis has long been limited by the unstable supply of natural prey, poor rearing of alternative prey and artificial diets, and high costs. In this paper, we review the research progress on the effects of alternative prey and artificial diets, and present the results and shortcomings of H. axyridis rearing. Focusing on the nutritional requirements of H. axyridis and the nutrient composition of natural prey, the paper aims to analyze and optimize the core components of artificial diets, develop diet formulations that are highly efficient, low-cost, support the ladybird throughout its complete life cycle, and identify the key factors for breaking the bottleneck of large-scale reproduction of H. axyridis, with a view to facilitate the industrialization of H. axyridis, enhancing the efficacy of biocontrol, and providing references to the development of diets for other natural enemy insects. Full article

Conventional alligator farming, characterized by reliance on chilled fish meat, faces significant challenges, including risks of bacterial contamination and nutritional imbalances. These issues heighten increasing disease susceptibility and threaten industry sustainability, underscoring the critical need for developing nutrient-dense, low-pathogenicity compound feeds. This study conducted a comparative analysis of newborn Siamese crocodiles fed either chilled fish meat or compound feed formulation. Intestinal microbial samples from both cohorts underwent 16S rRNA gene high-throughput sequencing to evaluate differences in microbial composition, diversity, and predicted functionality. The compound feed, specifically formulated for this investigation, possessed the following nutritional composition: crude protein 52.42%; digestible crude protein/digestible energy 16 mg/kcal; crude fat 12.31%; ash 17.42%; crude fiber 0.45%; starch 7.69%; digestible energy 3450 kcal/kg; lysine 3.66%; threonine 1.92%; methionine 1.27%; arginine 3.07%; total essential amino acids 22.97%; calcium 2.51%; total phosphorus 1.8%; available phosphorus 0.98%. Bioinformatics analysis revealed that the compound feed group exhibited numerically higher richness and alpha diversity indices within the intestinal microbiota compared to the chilled fish group. The microbial communities in both groups were dominated by the phyla Proteobacteria, Bacteroidetes, Fusobacteriota, and Firmicutes, collectively representing over 50% of the relative abundance. Functional prediction indicated that the compound feed group possessed the highest relative abundance in metabolic pathways associated with cofactor and vitamin metabolism, carbohydrate metabolism, amino acid metabolism, terpenoid and polyketide metabolism, lipid metabolism, and replication and repair. In contrast, the chilled fish group exhibited significant functional alterations in glycan biosynthesis and metabolism, translation, nucleotide metabolism, transcription, and biosynthesis of other secondary metabolites. Histomorphological analysis demonstrated greater villus height and crypt depth in the compound diet group compared to chilled fish group, although no significant differences were observed in crypt depth or the villus-to-crypt depth ratio. Collectively, these findings indicate that the compound feed enhances intestinal microbial diversity and optimizes its functional structure. Furthermore, while no statistically significant difference in small intestinal villus height was detected, the results suggest a potential positive influence on intestinal development. This investigation provides a scientific foundation for compound feed development, supporting sustainable breeding practices for Siamese crocodiles. Full article

Bio-based fertilisers (BBFs), produced from various types of biological waste using different processing methods, have demonstrated encouraging levels of agronomic efficiency and environmental benefits, consistent with the principles of sustainable development (SD). Nevertheless, bringing these newly developed products to market remains difficult due to limited farmer awareness, perceived risks, and regulatory uncertainties. In this paper, we examine the attitudes, opinions, and awareness of farmers regarding the use of various BBFs in their fertilisation practices. We applied a survey research method, using the Paper and Pen Personal Interview (PAPI), and answers were collected by agricultural advisors. A questionnaire, consisting of open, closed, and Likert scale questions, focusing on general information about farmers, current practices regarding fertiliser use, and the determinants of fertiliser choice, was used. Descriptive statistics, cross-tabulations, chi-square tests, Cramer’s V coefficients, 95% confidence intervals, and McNemar’s test were used to analyse the data. This study was conducted in all 16 voivodeships in Poland, collecting a total of 800 responses. Factors influencing the negative or positive attitude of farmers toward this practice were identified. Currently, mineral fertilisers remain the dominant choice among Polish farmers due to their accessibility, cost-effectiveness, and agronomic performance. There is observed growing, albeit cautious, interest in alternative fertilisation strategies and the correct understanding of sustainable agriculture practices. About half of farmers expressed willingness to partially replace mineral fertilisers with organic options, but only a minority showed interest in adopting BBFs. The findings indicate that concerns about contaminants, heterogeneous quality, limited availability, and regulatory uncertainty continue to constrain interest in BBFs. Although respondents recognised potential environmental benefits, economic and agronomic considerations remained the primary drivers of decision-making. As the survey was conducted in late 2021, the results reflect pre-2022 market conditions and should be interpreted as a baseline rather than a direct indication of current attitudes. Overall, this study provides insights into behavioural and structural factors influencing fertiliser use in Poland and highlights areas where further policy, advisory, and market developments may help support more sustainable nutrient management. Full article

The rising global burden of chronic diseases highlights the limitations of traditional dietary guidelines. Precision Nutrition (PN) aims to deliver personalized dietary advice to optimize individual health, and the effective implementation of PN fundamentally relies on comprehensive and accurate dietary data. However, conventional dietary assessment methods often suffer from quantification errors and poor adaptability to dynamic changes, leading to inaccurate data and ineffective guidance. Machine learning (ML) offers a powerful suite of tools to address these limitations, enabling a paradigm shift across the nutritional management pipeline. Using dietary data as a thematic thread, this article outlines this transformation and synthesizes recent advances across dietary assessment, in-depth mining, and nutritional intervention. Additionally, current challenges and future trends in this domain are also further discussed. ML is driving a critical shift from a subjective, static mode to an objective, dynamic, and personalized paradigm, enabling a loop nutrition management framework. Precise food recognition and nutrient estimation can be implemented automatically with ML techniques like computer vision (CV) and natural language processing (NLP). Integrating with multiple data sources, ML is conducive to uncovering dietary patterns, assessing nutritional status, and deciphering intricate nutritional mechanisms. It also facilitates the development of personalized dietary intervention strategies tailored to individual needs, while enabling adaptive optimization based on users’ feedback and intervention effectiveness. Although challenges regarding data privacy and model interpretability persist, ML undeniably constitutes the vital technical support for advancing PN into practical reality. Full article

One major bottleneck for the sustainable development of the aquaculture sector is the reliance on conventional feed ingredients, such as fishmeal and soy protein. Another challenge is nutrient loss from these systems, which contributes to environmental pollution but also represents a waste of valuable resources. To make aquaculture truly sustainable, a shift toward circular, sustainable systems is necessary. This study compared a regionally available alternative feed, based on blue mussel meal and pea protein concentrate, to a conventional fish meal and soybean control diet in Nile tilapia (Oreochromis niloticus) reared in coupled aquaponic systems. Fish performance and stress levels, water quality, plant growth, and microbial quality were investigated. Growth performance and feed intake were similar between aquaponic and control recirculating aquaculture systems (RASs) during the control feed (CF) phase. Only the feed conversion ratio (FCR) was slightly lower in the aquaponic system during the mussel-pea feed (MPF) phase. Tatsoi (Brassica rapa) growth in the aquaponic systems was comparable to, or even greater than, that of the hydroponic control systems, throughout the experiment, especially during the MPF phase. In addition, the MPF had a positive impact on phenotypic parameters and contributed to enhanced shoot growth. However, the presence of pathogens with potential biohazard impacts on human and fish health remains a concern and warrants further investigation. In our study, Salmonella spp. was detected in both systems, but levels were considerably reduced with the MPF phase. In contrast, Escherichia coli was detected only in RASs and was absent from aquaponic systems. Overall, the findings support the potential of blue mussel and pea protein as sustainable, local feed components in integrated aquaponic production, contributing to nutrient circularity and reducing dependence on limited marine stocks and imported resources. Full article

Background: Optimal nutritional care is essential to improving outcomes in hemodialysis, yet translation of evidence into routine practice remains uneven across settings. To inform health system planning and implementation priorities, we mapped global research on hemodialysis-related nutrition. Methods: We searched the Web of Science Core Collection for English-language original articles on nutrition and hemodialysis from 1 January 2006 to 13 October 2025. Publication trends, productivity by country and institution, influential journals and authors, citation impact, and conceptual structure via Keyword Plus co-occurrence, trend, and thematic evolution analyses were assessed using the bibliometrix package (version 5.0) in R. Results: A total of 332 articles from 115 journals were identified, with substantial growth and multidisciplinary authorship, though international collaboration remains limited. The United States contributed 21.4% of publications and achieved the highest citation impact, while China, Japan, Iran, and Brazil formed the next tier of contributors. The Journal of Renal Nutrition accounted for 16.6% of papers. Highly cited studies established links between dietary intake, mineral and electrolyte management, and survival, while supporting the use of intradialytic oral nutritional supplements. Thematic evolution showed a shift from biochemical markers toward patient-centered priorities, including diet quality, adherence, body composition, mental health, and quality of life. Emerging directions point to whole-diet approaches and microbiome-modulating strategies. Conclusions: Global research on diet and hemodialysis has progressed from foundational nutrient studies to multidimensional, patient-focused approaches. Our findings suggest opportunities for health systems to strengthen dietitian-led models of care, integrate patient-reported outcomes, and prioritize scalable nutrition interventions within routine dialysis services. Full article

Background: Chinese Yellow Earth is a key subtropical agricultural resource in southwestern China; however, its productivity is limited by acidity and poor nutrient retention. This study examined how reduced nitrogen plus organic amendments affect its soil microbial structure and maize yield. Methods: A field experiment with four treatments evaluated reduced nitrogen fertilization amended with rice husk plus rapeseed cake (RS) or RS with biochar (BC). Soil properties (pH, nitrogen, organic matter) and maize yield were analyzed. Metagenomic analysis (NR database) characterized microbial communities, and correlation analysis with Mantel tests identified key relationships. Results: Combined organic amendments under reduced N significantly increased soil pH, nitrogen components, and organic matter, increasing maize yield by 4.41–8.97%. Metagenomics revealed enriched beneficial genera including Sphingomonas and Bradyrhizobium. Yield positively correlated with nitrate nitrogen and a beneficial microbial cluster containing Lysobacter and Reyranella, whereas Steroidobacter negatively correlated with key fertility indicators. Mantel tests revealed nitrate nitrogen as the primary correlate of functional gene community succession. Conclusions: This study reveals that reduced nitrogen with organic amendments promotes soil improvement and microbial modulation, demonstrating potential as a sustainable practice to maintain crop productivity in Chinese Yellow Earth. The observed trend toward yield improvement underscores its promise and warrants further validation through additional trials. Overall, the findings highlight the beneficial effects of these amendments on soil health and their role in supporting sustainable subtropical agriculture under reduced nitrogen input. Full article

CAR T cell therapy, while highly effective for hematological malignancies, continues to face significant hurdles in the treatment of solid tumors. Key challenges include severe nutrient deprivation and the presence of immunosuppressive metabolites such as adenosine in the tumor microenvironment, which limit CAR T cell persistence and antitumor activity. This review focuses on current progress and future directions for ADA1-based metabolic reprogramming as a targeted approach to enhance CAR T cell function. We discuss recent advances, particularly the engineering of CAR T cells to express ADA1, which facilitates the local conversion of immunosuppressive adenosine into inosine, thereby supporting T cell metabolism and improving therapeutic outcomes. Preclinical studies, including our own, demonstrate that ADA1-expressing CAR T cells exhibit reduced exhaustion, greater metabolic flexibility, and enhanced antitumor efficacy in solid tumor models. The selective clearance of adenosine and supplementation of inosine directly address the metabolic barriers within the tumor microenvironment and provide an effective strategy to bolster CAR T cell responses. Integration of ADA1-driven metabolic refueling with future innovations in CAR design holds promise for overcoming key obstacles in solid tumor immunotherapy. We conclude by highlighting the potential of ADA1-based strategies and offering our perspective on their translation toward clinical application. Full article

Burkholderia is a metabolically versatile genus of Gram-negative bacteria that inhabits niches ranging from soil and water to plants and clinical environments. This review provides an integrated examination of Burkholderia species, focusing on their dual roles as both pathogens and beneficial microorganisms. Key pathogenic species, such as members of the Burkholderia cepacia complex and the Burkholderia pseudomallei group, pose significant threats to human, animal, and plant health due to their intrinsic antibiotic resistance and diverse virulence factors. Conversely, several environmental and plant-associated Burkholderia species promote plant growth, enhance nutrient uptake, and serve as biocontrol agents, supporting sustainable agriculture. We synthesize current knowledge across taxonomy, genomics, pathogenicity, beneficial interactions, and secondary metabolite biosynthesis—including the prolific production of antibiotics, toxins, and volatile organic compounds with pharmaceutical and agricultural potential. Advances in high-throughput genomics are revealing substantial genetic diversity, genome plasticity, and mechanisms underlying both pathogenicity and beneficial traits. Clarifying this dual nature and identifying strategies to mitigate risks will guide the safe and effective exploitation of Burkholderia in medicine, agriculture, and biotechnology. Full article

Ancient wheat cultivars play a crucial role in human and animal nutrition and health, serving as rich sources of bioactive compounds, essential nutrients, and functional metabolites. This study investigated Triticum turgidum subsp. durum (cv. Saragolla), an ancient wheat variety from the Campania region of Southern Italy, to comprehensively characterize its morphological, functional, and nutritional attributes in support of germplasm conservation and valorization. Standard AOAC methods, including HPLC profiling, antioxidant assays, and quantification of total polyphenols and flavonoids, were applied to characterize the grain’s composition. The results revealed a balanced distribution of proteins, lipids, dietary fibers, and carbohydrates, that defines the nutritional and functional quality of Saragolla grains. Microscopic investigation through SEM coupled with EDX analysis provided high-resolution visualization of caryopsis morphology, ultrastructure, and mineral distribution, confirming its distinct varietal characteristics. Additionally, SSR marker analysis revealed notable genetic diversity within the Saragolla germplasm, identifying loci associated with key agronomic traits, including kernel weight, grain number, and stress tolerance parameters essential for future breeding programs. Overall, this integrated assessment highlights Saragolla as a valuable heritage wheat and a strategic genetic resource for breeding durum cultivars with enhanced nutritional quality, technological performance, and resilience to environmental stress. Full article

Current food systems are highly complex, with interdependencies across regions, resources, and actors, and conventional food production is a major contributor to climate change. Transitioning to sustainable protein sources is therefore critical to meet the nutritional needs of a growing global population while reducing environmental pressures. Filamentous fungi present a promising solution by converting agro-industrial side streams into mycoproteins—nutrient-dense, sustainable proteins with a carbon footprint more than ten times lower than beef. This review evaluates the potential of mycoproteins derived from fungi cultivated on low-cost substrates, focusing on their role in advancing sustainable food systems. Evidence indicates that mycoproteins are rich in protein (13.6–71% dw), complete amino acids, fiber (4.8–25% dw), essential minerals, polyphenols, and vitamins while maintaining low fat and moderate carbohydrate content. Fermentation efficiency and product quality depend on substrate type, nutrient availability, and fungal strain, with advances in bioreactor design and AI-driven optimization enhancing scalability and traceability. Supported by emerging regulatory frameworks, mycoproteins can reduce reliance on animal-derived proteins, valorize agricultural by-products, and contribute to climate-resilient, nutritionally rich diets. Integration into innovative food products offers opportunities to meet consumer preferences while promoting environmentally sustainable, socially equitable, and economically viable food systems within planetary boundaries. Full article

Under global environmental change, the health of rivers and lakes on the “Asian Water Tower”—the Qinghai–Tibetan Plateau—is facing mounting pressures. This study examines Qinghai Lake, the Huangshui River, the Golmud River, and the Qinghai reach of the Yangtze River. By integrating the Water Quality Index (WQI) with the AHP–TOPSIS framework, we develop a multidimensional assessment system encompassing water resources, water environment, aquatic ecology, and management functions. The WQI results reveal pronounced spatial heterogeneity in water quality, with conditions ranked as Golmud River > Yangtze River > Huangshui River > Qinghai Lake. Dominant controlling factors also shift from dissolved oxygen in riverine systems to total phosphorus in the lake environment. The comprehensive AHP–TOPSIS evaluation further shows a health ranking of Yangtze River (0.736) > Golmud River (0.602) > Qinghai Lake (0.404) > Huangshui River (0.297), leading to the identification of four distinct management pathways: ecological conservation, natural restoration, nutrient control, and pollution remediation. By moving beyond single-parameter diagnostics, this study provides a robust methodological basis for differentiated river–lake management. The proposed “one river (lake), one strategy” framework, coupled with red-line management recommendations grounded in key indicators, offers direct scientific support for systematic protection and precise governance of aquatic ecosystems on the Qinghai–Tibetan Plateau, contributing to national ecological security and high-level environmental stewardship. Full article