Search Results (515)

Urochloa brizantha (Brizantha) is cultivated under varying altitudinal and management conditions. Twelve full-sun (monoculture) plots and twelve shaded (silvopastoral) plots were established, proportionally distributed at 170, 503, 661, and 1110 masl. Evaluations were conducted 15, 30, 45, 60, and 75 days after establishment. The conservation and integration of trees in silvopastoral systems reflected a clear anthropogenic influence, evidenced by the preference for species of the Fabaceae family, likely due to their multipurpose nature. Although the altitudinal gradient did not show direct effects on soil properties, intermediate altitudes revealed a significant role of CaCO₃ in enhancing soil fertility. These edaphic conditions at mid-altitudes favored the leaf area development of Brizantha, particularly during the early growth stages, as indicated by significantly larger values (p < 0.05). However, at the harvest stage, no significant differences were observed in physiological or productive traits, nor in foliar chemical components, underscoring the species’ high hardiness and broad adaptation to both soil and altitude conditions. In Brizantha, a significant reduction (p < 0.05) in stomatal size and density was observed under shade in silvopastoral areas, where solar radiation and air temperature decreased, while relative humidity increased. Nonetheless, these microclimatic variations did not lead to significant changes in foliar chemistry, growth variables, or biomass production, suggesting a high degree of adaptive plasticity to microclimatic fluctuations. Foliar ash content exhibited an increasing trend with altitude, indicating greater efficiency of Brizantha in absorbing calcium, phosphorus, and potassium at higher altitudes, possibly linked to more favorable edaphoclimatic conditions for nutrient uptake. Finally, forage quality declined with plant age, as evidenced by reductions in protein, ash, and In Vitro Dry Matter Digestibility (IVDMD), alongside increases in fiber, Neutral Detergent Fiber (NDF), and Acid Detergent Fiber (ADF). These findings support the recommendation of cutting intervals between 30 and 45 days, during which Brizantha displays a more favorable nutritional profile, higher digestibility, and consequently, greater value for animal feeding. Full article

Cover cropping has emerged as a pivotal sustainable agronomic practice aimed at enhancing soil health and sustaining crop productivity. To quantify its effects across diverse agroecosystems, we conducted a meta-analysis of 1877 paired observations from 114 studies (1980–2025) comparing cover cropping with bare fallow during fallow periods in major cereal systems across China. Cover cropping significantly reduced soil bulk density by 6.1% and increased key soil nutrients including total nitrogen (+13.1%), total phosphorus (+15.6%), hydrolysable nitrogen (+9.3%), available phosphorus (+11.1%), available potassium (+12.4%), soil organic matter (+11.7%), and microbial biomass carbon (+41.1%). Leguminous cover crops outperformed non-legumes in enhancing nitrogen availability, reflecting biological nitrogen fixation. Mixed-species cover crop mixtures showed superior benefits over monocultures, likely due to complementary effects on nutrient cycling and soil structure. Soil texture and initial soil organic carbon significantly moderated these outcomes. Furthermore, although overall soil pH remained stable, cover cropping exhibited a clear buffering effect, tending to regulate soil pH toward neutrality. Meta-regression analyses revealed a diminishing positive effect on total nitrogen (TN), available potassium (AK), and microbial biomass carbon (MBC) with an extended duration of cover cropping, suggesting potential saturation effects. These results underscore the context-dependent efficacy of cover cropping as a strategy for soil quality enhancement. Optimizing cover crop implementation should integrate the consideration of inherent soil characteristics, baseline fertility, and species composition to maximize agroecosystem resilience and sustainability. Full article

This study evaluated the effects of different doses of limonene essential oil (LEO) and a blend of cinnamaldehyde and carvacrol (BCC) on the fermentative quality and chemical–bromatological composition of total mixed ration (TMR) silages. Two independent trials were conducted, each focused on one additive, using a completely randomized design with four treatments (0, 200, 400, and 600 mg/kg of dry matter), replicated across two seasons (summer and autumn), with five replicates per treatment per season. The silages were assessed for their chemical composition, fermentation profile, aerobic stability (AS), and storage losses. In the LEO trial, the dry matter (DM) content increased significantly by 0.047% for each mg/kg added. Dry matter recovery (DMR) peaked at 97.9% at 473 mg/kg (p < 0.01), while lactic acid (LA) production reached 5.87% DM at 456 mg/kg. Ethanol concentrations decreased to 0.13% DM at 392 mg/kg (p = 0.04). The highest AS value (114 h) was observed at 203.7 mg/kg, but AS declined slightly at the highest LEO dose (600 mg/kg). No significant effects were observed for the pH, neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), or non-fiber carbohydrates (NFCs). In the BCC trial, DMR reached 98.2% at 548 mg/kg (p < 0.001), and effluent losses decreased by approximately 20 kg/ton DM. LA production peaked at 6.41% DM at 412 mg/kg (p < 0.001), and AS reached 131 h at 359 mg/kg. BCC increased NDF (from 23.27% to 27.73%) and ADF (from 35.13% to 41.20%) linearly, while NFCs and the total digestible nutrients (TDN) decreased by 0.0007% and 0.039% per mg of BCC, respectively. In conclusion, both additives improved the fermentation efficiency by increasing LA and reducing losses. LEO was more effective for DM retention and ethanol reduction, while BCC improved DMR and AS, with distinct effects on fiber and energy fractions. Full article

Our previous study has shown that supplementation of 0.50% benzoic acid (BA) increased growth performance, promoted rumen fermentation, and improved the composition and function of rumen microbiota. This research was designed to conduct a deeper exploration of the impacts of dietary supplementation with BA on the apparent digestibility of nutrients and the composition of rectal microbiota in weaned Holstein dairy calves. Sixteen Holstein heifer calves with similar body weights (91.2 ± 0.7 kg) were selected and randomly allocated into two groups, each comprising eight calves. Calves in the control group (CON group) were fed with a basal diet, while those in the benzoic acid group (BA group) were fed with the basal diet supplemented with 0.50% benzoic acid (on a dry matter basis). The experimental period started at 60 days of age and ended at 102 days of age, lasting for a total of 42 days. The calves were weaned at 60 days of age, with a transition period of 7 days. Feed samples were collected every two weeks, fecal samples were collected from 99 to 101 days of age, and blood samples were collected at 102 days of age. The results showed that supplementation with BA did not influence the digestibility of dry matter, crude protein, ether extract, neutral detergent fiber, acid detergent fiber, calcium, and phosphorus between the two groups. Compared with the CON group, BA supplementation tended to decrease the total cholesterol (TC) in the serum of the calves (p = 0.067). Supplementation with BA increased the relative abundances of the two beneficial bacteria, Bifidobacterium and Bifidobacterium pseudolongum (p < 0.05, LDA > 2), but decreased that of the harmful bacterium, Clostridium sensu stricto 1, in the rectum of dairy calves. The microbial functional prediction revealed that the fecal microbial metabolism involved in primary bile acid biosynthesis was higher in the calves from the BA group. In conclusion, the present study demonstrated that adding 0.50% BA to the diet did not influence the apparent nutrient digestibility, but improved rectal microbiota health, which finally promoted the growth performance in weaned Holstein dairy calves. Full article

The objective of this study is to investigate the degradation characteristics of oat grass in the rumen of Mindong goats and changes in microbial community attached to the grass surface. Four healthy male goats, aged 14 months, with permanent rumen fistula, in eastern Fujian, were selected as experimental animals. The rumen degradation rate of oat grass was measured at 4, 12, 24, 36, 48, and 72 h using the nylon bag method. Surface physical structure changes in oat grass were observed using scanning electron microscopy (SEM), cellulase activity was measured, and bacterial composition was analyzed using high-throughput 16S rRNA gene sequencing technology. The findings of this study indicate that oat grass had effective degradation rates (ED) of 47.94%, 48.69%, 38.41%, and 30.24% for dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and acidic detergent fiber (ADF), respectively. The SEM was used to investigate the degradation process of oat grass in the rumen. After 24 h, extensive degradation of non-lignified tissue was observed, resulting in the formation of cavities. At 36 h, significant shedding was observed, and by 72 h, only the epidermis and thick-walled tissue, which exhibited resistance to degradation, remained intact. Surface-attached microorganisms produced β-GC, EG, CBH, and NEX enzymes. The activity of these enzymes exhibited a significant increase between 4 and 12 h and showed a positive correlation with the degradation rate of nutrients. However, the extent of correlation varied. Prevotella and Treponema were identified as key genera involved in the degradation of roughage, with their abundance decreasing over time. Principle Coordinate Analysis (PCOA) revealed no significant differences in the rumen microbial structure across different time points. However, Non-Metric Multidimensional Scaling (NMDS) indicated a discernible diversity order among the samples. According to the Spearman correlation coefficient test, Ruminococcus, Fibrobacter, and Saccharoferments exhibited the closest relationship with nutrient degradation rate and surface enzyme activity, displaying a significant positive correlation. In summary, this study delineates a time-resolved correlative framework linking microbial succession to structural and enzymatic dynamics during oat grass degradation. Full article

In accordance with growing environmental pressures and the demand for sustainable industrial practices, membrane technologies have emerged as key enablers for increasing efficiency, reducing emissions, and supporting circular processes across multiple sectors. This review focuses on the integration among microalgae-based systems, offering innovative and sustainable solutions for biomass production, carbon capture, and industrial wastewater treatment. In cultivation, membrane photobioreactors (MPBRs) have demonstrated biomass productivity up to nine times greater than that of conventional systems and significant reductions in water (above 75%) and energy (approximately 0.75 kWh/m³) footprints. For carbon capture, hollow fiber membranes and hybrid configurations increase CO₂ transfer rates by up to 300%, achieving utilization efficiencies above 85%. Coupling membrane systems with industrial effluents has enabled nutrient removal efficiencies of up to 97% for nitrogen and 93% for phosphorus, contributing to environmental remediation and resource recovery. This review also highlights recent innovations, such as self-forming dynamic membranes, magnetically induced vibration systems, antifouling surface modifications, and advanced control strategies that optimize process performance and energy use. These advancements position membrane-based microalgae systems as promising platforms for carbon-neutral biorefineries and sustainable industrial operations, particularly in the oil and gas, mining, and environmental technology sectors, which are aligned with global climate goals and the UN Sustainable Development Goals (SDGs). Full article

Eutrophication driven by nitrogen and phosphorus discharge remains a critical global environmental challenge. This study developed a sustainable strategy for synergistic nutrient removal and recovery by fabricating MgO-coated biochar (Mg-MBC600) through co-pyrolysis of municipal sludge and sunflower stalk (300–700 °C). Systematic investigations revealed temperature-dependent adsorption performance, with optimal nutrient removal achieved at 600 °C pyrolysis. The Mg-MBC600 composite exhibited enhanced physicochemical properties, including a specific surface area of 156.08 m²/g and pore volume of 0.1829 cm³/g, attributable to magnesium-induced structural modifications. Advanced characterization confirmed the homogeneous dispersion of MgO nanoparticles (~50 nm) across carbon matrices, forming active sites for chemisorption via electron-sharing interactions. The maximum adsorption capacities of Mg-MBC600 for nitrogen and phosphorus reached 84.92 mg/L and 182.27 mg/L, respectively. Adsorption kinetics adhered to the pseudo-second-order model, indicating rate-limiting chemical bonding mechanisms. Equilibrium studies demonstrated hybrid monolayer–multilayer adsorption. Solution pH exerted dual-phase control: acidic conditions (pH 3–5) favored phosphate removal through Mg₃(PO₄)₂ precipitation, while neutral–alkaline conditions (pH 7–8) promoted NH₄⁺ adsorption via MgNH₄PO₄ crystallization. XPS analysis verified that MgO-mediated chemical precipitation and surface complexation dominated nutrient immobilization. This approach establishes a circular economy framework by converting waste biomass into multifunctional adsorbents, simultaneously addressing sludge management challenges and enabling eco-friendly wastewater remediation. Full article

Mycorrhizal symbiosis in rice enhances drought adaptation but there are limited studies regarding the frequency and amplitude of mycorrhizae colonization in traditional landraces. This study investigates mycorrhizal colonization frequency (FMS) and intensity (IRS) in 12 rice landraces across three agroecological zones (Tarai, Inner-Tarai, Mid-hill) of Far-West Nepal under drought stress. Field experiments exposed landraces to control, intermittent, and complete drought treatments, with soil properties and root colonization analyzed. Results revealed FMS and IRS variations driven by soil composition and genotype. Mid-hill soils (acidic, high organic matter) showed lower FMS but elevated IRS under drought, while neutral pH in Tarai and silt/clay-rich soils supported higher FMS. Sandy soil in Inner-Tarai also promoted FMS. Drought significantly increased IRS, particularly in Anjana and Sauthiyari (Tarai), Chiudi and Shanti (Inner-Tarai), and Chamade and Jhumke (Mid-hill), which exhibited IRS surges of 171–388%. These landraces demonstrated symbiotic resilience, linking mycorrhizal networks to enhanced nutrient/water uptake. Soil organic matter and nutrient levels amplified IRS responses, underscoring fertility’s role in adaptation. FMS ranged from 50 to 100%, and IRS 1.20–19.74%, with intensity being a stronger drought-tolerance indicator than frequency. The study highlights the conservation urgency for these landraces, as traditional varieties decline due to hybrid adoption. Their drought-inducible mycorrhizal symbiosis offers a sustainable strategy for climate-resilient rice production, emphasizing soil–genotype interactions in agroecological adaptation. Full article

Background: The current study aimed to evaluate the effects of the supplementation of protease sources on growth and carcass response, gut health, nutrient digestibility, and cecal microbiota profiles in broilers fed poultry-by-product-meal (PBM)-containing diets. Methods: In total, 800 one-day-old mixed-sex broilers (Arbor Acres) were weighed and allocated to one of the four dietary treatments in a completely randomized design, with eight replicates and 25 birds each per replicate. The treatments were as follows: (1) T₀, control diet (without protease supplementation and 3% PBM); (2) T₁, control diet supplemented with acidic protease at 100 g/ton (50,000 U/g); (3) T₂, control diet supplemented with alkaline protease at 200 g/ton (25,000 U/g); (4) T₃, control diet supplemented with neutral protease at 200 g/ton (25,000 U/g). Results: Protease supplementation enhanced (p < 0.05) body weight gain and the feed conversion ratio, predominantly in broilers fed PBM-based diets containing alkaline protease. Alkaline protease supplementation increased (p < 0.05) the apparent ileal digestibility of proteins (AIDP) by 4.3% and the apparent ileal digestibility of amino acids (AIDAA) by up to 5.8%, except for ornithine. Increments (p < 0.05) in carcass, breast, and leg quarter yields due to protease supplementation were evident, particularly in broilers fed diets containing alkaline protease. Alkaline protease improved (p < 0.05) the duodenal villus height (VH), reduced the crypt depth (CD), and increased the villus height to crypt depth ratio (VCR). Alkaline protease supplementation reduced (p < 0.05) cecal counts of Salmonella, Escherichia coli, and Clostridium in the broilers, whereas it increased (p < 0.05) the Lactobacillus counts. Conclusions: the supplemented alkaline protease resulted in improved growth performance and carcass traits, better gut health, as well as improved ileal digestibility of nutrients, including crude protein (CP) and acid insoluble ash (AIA), with a more balanced cecal microbial composition in broilers. Full article

The drive towards carbon neutrality has prompted the worldwide expansion of utility-scale solar facilities. Previous studies have reported the positive effects of solar facilities’ installation on pasture productivity and biodiversity in arid regions. However, our understanding of how solar facilities influence a wide range of ecosystem functions simultaneously, and the relative contributions of soil microbial attributes, remains incomplete. To address this gap, we assessed the changes in ecosystem multifunctionality following solar facility installation in an alpine desert grassland in the Qinghai–Tibet plateau by measuring twenty-three ecosystem function indicators comprising primary production, the soil nutrient pool, carbon cycling, nitrogen cycling, phosphorus cycling and oxidation–reduction. Furthermore, we estimated the soil microbial diversity, microbial indicator taxa and microbial activity to identify the primary driving factors. The results showed that solar facilities had positive effects on ecosystem multifunctionality; the positive effect size was higher in the initial installation period (31.4%) than in the constant running period (3.5%). The enhancements in ecosystem multifunctionality were mainly due to enhanced nutrient cycling induced by the increased abundance of fungal indicator taxa and microbial activity. Moreover, the structural equation model revealed distinct regulatory paths between the two periods and a transition in the primary driving factors of ecosystem multifunctionality from microbial indicator taxa to microbial activity. In conclusion, our study demonstrates the positive influence of solar facilities on multiple ecosystem functions, emphasizing the critical role of soil microbial mechanisms in regulating ecosystem multifunctionality. These findings provide valuable insights into soil biota-driven processes that could inform strategies aimed at enhancing soil health and ecosystem functionality in arid grasslands under human-managed systems. Full article

The utilization of nettle (Urtica cannabina) as feed is restricted by its material properties (antibacterial activity and high buffering capacity). This study hypothesized that the use of lactic acid bacteria (LAB) attached to nettles can improve these problems. Lactococcus garvieae (LG), Pediococcus pentosaceus (PP), and LG + PP (LP) isolated from nettles were inoculated into nettle silage to explore nutrient retention and the microbial community structure. The results showed that inoculation significantly delayed dry matter and crude protein loss, inhibited neutral detergent fiber and acid detergent fiber degradation, and reduced ammonia nitrogen (NH₃-N) accumulation. There was a significant increase in Firmicutes abundance after inoculation, and the dominant genus, Aerococcus, was negatively correlated with NH₃-N accumulation. In the later stages of the PP treatment, Atopistipes synergistically inhibited Clostridia with acetic acid. However, the high buffering capacity and antibacterial components of raw nettle led to increased pH values during the later fermentation stages, limiting sustained acid production by LAB. These results confirm that nettle-derived LAB can effectively improve the quality of silage by regulating the microbial community and the acidification process; however, they must be combined with pretreatment strategies or optimized composite microbial agents to overcome raw material limitations. This study provides a theoretical basis and technical support for the utilization of nettle as feed. Full article

Utilizing agro-industrial by-products to feed livestock is crucial for environmental protection and, simultaneously, lowering production and feeding costs. In light of these aims, in this study, two trials were conducted to evaluate the impact of jojoba meal (JJM) on Awassi ewes’ milk properties, nutrient intake, digestibility, and the pre-weaning growth of their nursing lambs. In the first trial, 22 Awassi ewes were divided equally between two experimental diets at random (11 ewes per diet): 0% JJM (CON) and 15% JJM (JJM15) of dietary dry matter (DM). In the second trial, 10 ewes were randomly selected (5 ewes/diet) to assess nutritional digestibility and N balance. The results of trial 1 showed that neutral detergent fiber (NDF), acid detergent fiber (ADF), and ether extract (EE) intake values were greater (p ≤ 0.02) in the JJM15 group compared to the CON group. The total gain and average daily gain (ADG) of the lambs in the JJM15 group were significantly higher compared to those of the CON group (p < 0.05). The average milk yield of nursing ewes was similar between the two dietary groups (p ˃ 0.05). The percentage and yield of solids-not-fat (SNF) in the JJM15 group were significantly higher compared to the CON group. The other milk components, including protein, lactose, fat, and total solids yields, were similar between the dietary groups (p ˃ 0.05). The milk production cost was significantly lower for the JJM15 diet than the CON diet (p = 0.004). Triglyceride levels were lower (p = 0.001) in the JJM15 diet group than in the CON diet group. In trial 2, nutrient digestibility and N balance were not affected (p ≥ 0.073) by the consumption of JJM15. In conclusion, JJM at 15% of dietary DM resulted in no negative effects on the health, performance, or milk quality of Awassi ewes. Most significantly, by lowering the cost of production, our results appear to indicate that this dietary supplement improves economic efficiency. Full article

Whey, a large-scale dairy industry by-product, can be converted into whey protein concentrate (WPC), providing a cost-effective nutrient-rich substrate for microbial fermentation. We investigated protease production by Aspergillus oryzae using WPC as the sole substrate in submerged fermentation. Following fermentation, the protease was purified sequentially from the crude extract by salting-out, which yielded a substantial purification factor (~39), and subsequent ion-exchange chromatography. The non-adsorbed chromatographic fraction showed the highest protease activity (92.6 U/mL) and revealed one main protein band ~45 kDa via SDS-PAGE. Enzyme characterization demonstrated activity across neutral-to-alkaline conditions, optimal at pH 9.0 and 37 °C, with stability maintained between 30 °C and 37 °C. The enzyme was classified as a serine protease based on strong inhibition by PMSF and SDS; its activity was also inhibited by Zn²⁺, Mg²⁺, and K⁺, but enhanced by Ca²⁺. This work validates WPC as an efficient substrate for protease production by A. oryzae and presents a promising strategy for valorizing industrial by-products through sustainable biotechnology. Full article

Dairy cows are suffering from heat stress (HS) worldwide, and this has become a continual challenge in dairy production systems. The objective of this study was to investigate the effects of the shade of poplar trees on alleviating HS via measuring milk production, nutrient digestibility, immunity, and antioxidant capability in the serum of dairy cows in open cowsheds. A total of 540 lactating Holstein cows were assigned to 2 groups (3 cowsheds per group, 90 cows per cowshed), including the treatment group with poplar trees on the west side of sheds and the control group without trees. This study was carried out in the early-hot season, hot season, and late-hot season. The results showed the following: (1) During the hot season, shaded cows exhibited lower respiration rate (p < 0.05) and higher dry matter intake (p < 0.05) and daily milk yield (p < 0.05), compared with the control. Also, apparent digestibility of crude protein, acid detergent fiber, and neutral detergent fiber demonstrated an increase (p < 0.05) in shaded cows during the hot season. (2) Total antioxidant capacity and superoxide dismutase activity in the serum of shaded cows increased (p < 0.05) during the hot season and late-hot season, compared with the control. (3) Serum interleukin-4, immunoglobulin G, and immunoglobulin M concentrations of shaded cows were greater (p < 0.05) than the control during the hot season. Additionally, serum concentrations of HSP60, HSP70, and HSP90 in shaded cows showed a decrease (p < 0.05) during the hot season. In conclusion, shade from poplar trees can mitigate the adverse impact of high-temperature environments on cows in open cowsheds. Full article

Excess phosphorus (P) and nitrogen (N) in wastewater contribute to eutrophication, driving the need for low–cost and sustainable recovery technologies. This study presents a novel adsorbent synthesized from spent coffee grounds biochar (CB) chemically modified with Mn²⁺/Zn²⁺/Fe³⁺ (oxy)hydroxide nanoparticles (CB–M) for simultaneous removal of phosphate and ammonium. Batch adsorption experiments using both synthetic solution and municipal wastewater were conducted to evaluate the material’s adsorption performance and practical applicability. Kinetic, isotherm, thermodynamic, and sequential extraction analyses revealed that CB–M achieved maximum phosphate adsorption capacities ranging from 42.6 to 72.0 mg PO₄³⁻·g⁻¹ across temperatures of 20–33 °C, reducing effluent phosphate concentrations to below 0.01 mg·L⁻¹. Ammonium removal was moderate, with capacities ranging between 2.8 and 2.95 mg NH₄⁺·g⁻¹. Thermodynamic analysis indicated that phosphate adsorption was spontaneous and endothermic, dominated by inner–sphere complexation, while ammonium uptake occurred primarily through weaker, reversible ion exchange mechanisms. Sequential extraction showed over 70% of adsorbed phosphate was associated with Fe-Mn-Zn phases, indicating the potential for use as a slow–release fertilizer. The CB–M retained structural integrity and exhibited partial desorption, supporting its reusability for nutrient recovery. Compared to other biochars, CB–M demonstrated superior phosphate selectivity at a neutral–pH, avoided the use of hazardous metals, and transformed coffee waste into a multifunctional material for wastewater treatment and soil amendment. These findings underscore the potential of CB–M as a circular economy solution for nutrient recovery without introducing secondary contamination. Full article