Search Results (62)

Comprehensive weekly load data appears relatively absent in the literature for the professional female soccer population. This exploratory observational study quantified the weekly training, match, and total loads experienced in a professional soccer team and compared these loads according to player role. Data were collected over a full season from 22 players competing in the Australian national A-League Women’s soccer competition. Internal (session-rating of perceived exertion load [session-RPE load]) and external load (total and relative values for total and high-speed running [HSR] distance) data were acquired during on-field training sessions and matches. Players were categorized as starters (started the match) or non-starters (used as a substitute in the match) within each week. Linear mixed models and effect size analyses were used to compare weekly loads between player roles. Weekly match and total load analyses revealed higher (p < 0.001, moderate-to-very large effects) duration, total distance, total HSR distance, and session-RPE load in starters than non-starters. In contrast, relative total and HSR distance in matches were higher (p < 0.001, moderate-to-large effects) in non-starters than in starters. These data provide an initial reference for the weekly loads in this competition, highlighting disparities between player roles that should be considered when developing training and preparation plans. Given the recruitment of a single team and small sample size, future research should extend upon this study to further strengthen the evidence base in this population. Full article

Cheddar cheese ripening creates favorable conditions for desired microbial changes but also allows survival and outgrowth of spores like Clostridium tyrobutyricum and Bacillus licheniformis, leading to late-blowing defects. In the first phase of the study, NSLAB dynamics were evaluated in the presence of spores, where pilot-scale cheeses (110 L) were produced in four treatments: control, T1 (BL), T2 (CT), and T3 (BL+CT), each inoculated at 2.0 Log₁₀ CFU/mL with spores. Results showed that SLAB declined from 8.0 to 0.2 Log₁₀ CFU/g, while NSLAB increased from 2.0 to 8.5 Log₁₀ CFU/g by the third month and remained stable thereafter. Spore counts reached 2.94 ± 0.02 (T2) and 2.48 ± 0.03 (T3) Log₁₀ CFU/g, with visible spoilage signs appearing after five months, indicating that native NSLAB populations were inadequate to control late-blowing defects. In this study, the effect of soluble fiber (inulin) in stimulating NSLAB was evaluated by incorporating 1% inulin into Cheddar cheese across four treatments: T1 (C SF), T2 (BL SF), T3 (CT SF), and T4 (BL+CT SF). Inulin addition resulted in significantly higher NSLAB counts (>10.5 Log₁₀ CFU/g) and suppressed spore levels (<0.91 ± 0.03 Log₁₀ CFU/g), with no spoilage observed. Inulin addition selectively enhanced beneficial NSLAB, suppressing spore-forming bacteria and preventing late-blowing defects without affecting cheese quality. This provides a natural, sustainable strategy to enhance microbial safety during Cheddar cheese ripening. Full article

Dairy products harbor complex and dynamic microbial communities that contribute to their sensory properties, safety, and cultural distinctiveness. Raw milk contains a diverse microbiota shaped by seasonality, storage conditions, lactation stage, animal health, farm management, and genetics, serving as a variable starting point for further processing. Fermentation, whether spontaneous or starter driven, selects for subsets of lactic acid bacteria (LAB), yeasts, and molds, resulting in microbial succession that underpins both artisanal and industrial products such as kefir and cheese. Kefir represents a balanced LAB–yeast symbiosis, with species composition influenced by grain origin, milk type, and processing parameters, whereas the cheese microbiota reflects the interplay of starter and non-starter LAB, coagulants, ripening conditions, and “house microbiota”. Methodological factors—including DNA extraction, sequencing platform, and bioinformatic pipelines—further impact the reported microbial profiles, highlighting the need for standardization across studies. This review synthesizes current knowledge on raw milk, kefir, and cheese microbiomes, emphasizing the biological, technological, environmental, and methodological factors shaping microbial diversity. A holistic understanding of these drivers is essential to preserve product authenticity, ensure safety, and harness microbial resources for innovation in dairy biotechnology. Full article

Cheese ripening involves microbial changes, with starter lactic acid bacteria (SLAB) initiating fermentation and nonstarter lactic acid bacteria (NSLAB) driving flavor and texture development. However, heat-resistant spores of Clostridium and Bacillus can survive pasteurization and cause spoilage during ripening. This study evaluated NSLAB dynamics in the presence of spores during cheese ripening. Cheddar cheese samples at pilot-scale level (110 L) with four treatments, namely control, with spores of B. licheniformis (T1), with spores of Cl. tyrobutyricum (T2), and both spores (T3) at 2.0 Log₁₀ CFU/mL, were ripened at 7 °C for six months. SLAB declined from 8.0 to 0.2 Log₁₀ CFU/g, while NSLAB increased from 2.0 to 8.5 Log₁₀ CFU/g by month three and maintained their counts up to six months, unaffected by spore presence. Spore counts were ≤1.45 Log₁₀ CFU/g in controls but reached 2.94 ± 0.02 (T2) and 2.48 ± 0.03 (T3), correlating with spoilage signs after five months. MALDI-TOF identified L. rhamnosus (up to 37%) and L. paracasei (up to 25%) as dominant NSLAB across treatments. Physicochemical parameters were not significantly affected by higher spore levels. While NSLAB dominated, they were inadequate to prevent spoilage in spore-inoculated samples exceeding 2.0 logs during cheese ripening. Full article

Background: Understanding the balance between intensity and volume during training and competition is crucial for optimizing players’ performance and recovery in professional soccer. While worst-case scenarios (WCSs) are commonly used to assess peak match demands, little is known about how the time spent within WCS thresholds varies across congested and non-congested periods, especially when considering differences in playing time. This study examines the time spent at different percentages of WCSs during congested and non-congested periods for players with lower and higher playing times throughout training sessions and matches. Methods: Data were collected from a professional soccer team across a congested and non-congested match period. Twenty players were divided into two groups based on playing time: the top 10 playing times (PT 1–10) and the bottom 10 playing times (PT 11–20). WCS thresholds for total distance (TD) and the distance covered above 20 km·h⁻¹ (D20) were quantified in 10% increments, starting from 50% and increasing up to >100%. The time spent at each threshold was compared between periods and groups for the integrated soccer exercises performed during all training sessions. Repeated measures of ANOVA were used to analyze differences between playing time groups and periods. Results: During training, players spent significantly more time within the 50–90% WCS TD and WCS D20 thresholds during non-congested periods compared to congested periods (p < 0.05). However, no significant differences were observed in the time spent for >90% of the WCSs between periods (p > 0.05). Both PT 1–10 and PT 11–20 groups exhibited similar patterns of WCS achievement, with small effect sizes observed for a few indicators. Conclusion: Coaches should design training sessions that replicate or exceed match demands, particularly during non-congested periods. Future strategies should integrate larger-sided games with longer durations and dissociated contents to better individualize and optimize training loads, especially for non-starters. Full article

This review contributes to the knowledge on the complex and adaptive microbial ecosystems within cheese, emphasizing their critical role in determining cheese quality, flavor, and safety. This review synthesizes the current knowledge on the microbial interactions and the dynamics of lactic acid bacteria (LAB), encompassing both starter (SLAB) and non-starter (NSLAB) strains, which are pivotal to the curd fermentation and ripening processes. The adaptability of these microbial consortia to environmental and technological stressors is explored, highlighting their contributions to acidification, proteolysis, and the development of distinctive organoleptic characteristics. Historical and technological perspectives on cheesemaking are also discussed, detailing the impact of milk treatment, starter culture selection, and post-renneting procedures on microbial activity and biochemical transformations. This review underscores the importance of microbial diversity and cooperative interactions in fostering ecosystem resilience and metabolic functionality, and it addresses the challenges in mimicking the technological performance of natural starters using selected cultures. By understanding the ecological roles and interactions of cheese microbiota, this review aims to guide improvements in cheese production practices. Additionally, these insights could spark the development of innovative strategies for microbial community management. Full article

The aim of this study was to analyze the effects of the playing position (backs vs. pivots vs. wings) and the player role (starter vs. non-starter) on the internal match load and well-being status of female handball players after official matches. Fourteen female handball players from the Spanish 2nd Division were monitored during a half-season (13 matches, n = 102 individual observations) using the rate of perceived exertion (match RPE) and the Hooper questionnaire in MD+1 and MD+2. Differences in match RPE according to playing positions and player roles were determined by one-way ANOVA or by a paired t-test, respectively. Differences in well-being status according to playing positions or player roles and time (MD+1 and MD+2) were assessed through mixed two-way ANOVA. Furthermore, partial eta-squared (ηp²) and Cohen’s d (ES) were calculated and interpreted using Hopkins’ categorization criteria. Backs registered moderately more match RPE compared to pivots (p < 0.05, ES = 0.84). By contrast, wings experienced the highest values of fatigue and muscle soreness in MD+1 and MD+2 compared to all other playing positions (p < 0.05, ES = 0.66–0.93). Also, the wings reported moderately higher Hooper index scores in MD+1 and MD+2 than backs (p < 0.01, ES = 0.73–0.77). In relation to the player role, starters reported higher absolute values of match RPE, fatigue, muscle soreness, and the Hooper index in MD+1 compared to non-starters (p < 0.05, ES = 0.25–0.29). Additionally, regardless of the playing position and the role, all of the players reported moderately less fatigue and Hooper index scores in MD+2 compared to MD+1 (p < 0.05, ES = 0.66–1.34). Therefore, coaches and practitioners should consider the internal match load and well-being status of players to implement different training stimuli (e.g., recovery or compensatory strategies) in MD+1 according to playing positions and player roles. Full article

Monitoring training load, recovery, and wellness in maturation ages is even more relevant than in adulthood, especially during congested schedules. The objective of this study was to quantify and describe the differences in external and internal load, recovery, and perceived wellness in elite under-16 female players during a period of three competitions in seven days based on their participation during matches. An observational design was used to quantify the external load (total distance, high-speed distance, and PlayerLoad) and internal load (differential sRPE), as well as the recovery and wellness of 18 international under-16 players during a development tournament. The external load pattern of the starting players was undulating, with matches showing the highest internal load values compared to training sessions and non-starters. Non-starter players (whether training or matches) showed similar external load and differential sRPE values throughout the tournament (p > 0.05). Starting players experienced higher internal load than non-starters during matches (p < 0.037), without differences between training sessions (p > 0.05). No changes were seen in wellness or recovery (all p-values > 0.05), except for non-starters in Session 3 compared to Session 7 (p = 0.024). Starting players had more weekly total minutes (p = 0.018), differential sRPE (breath: p = 0.002; leg: p = 0.002; cognitive: p = 0.003), total distance (p = 0.003), and PlayerLoad (p = 0.008) than non-starters. Our results indicate differing load patterns between starters and non-starters, which do not affect wellness and recovery differently. Starters accumulate more weekly total load. Coaches can use these findings to guide decisions and load compensations in high competition contexts for young female soccer players. Full article

Enterococci are a group of microorganisms that have a controversial position from some scientific points of view. The species of the greatest clinical importance are E. faecalis and E. faecium, which are common agents of nosocomial infections. However, enterococci also have important applications in the dairy industry, as they are used as non-starter lactic acid bacteria (NSLAB) in a variety of cheeses, especially artisanal cheeses. The aim of this study was to determine the presence of representatives from the Enterococcus genus using PCR and MALDI-TOF MS methods on samples of raw milk, processing environment swabs, and cheese from four different artisanal dairy plants in Slovakia. Among the 136 isolates of enterococci, 9 species of genus Enterococci (E. faecalis, E. faecium, E. durans, E. devriesi, E. hirae, E. italicus, E. casseliflavus, E. malodoratus, and E. gallinarum) were identified and were tested for their antimicrobial resistance (AMR) to 8 antibiotics (amoxicillin, penicillin, ampicillin, erythromycin, levofloxacin, vancomycin, rifampicin, and tetracycline); most of them were resistant to rifampicin (35.3%), ampicillin (22.8%), and tetracycline (19.9%). A PCR analysis of vanA (4.41%) and tetM (14.71%) revealed that antimicrobial resistance genes were present in not only phenotypic resistant isolates of enterococci but also susceptible isolates. The investigation of antimicrobial resistance in enterococci during the cheesemaking process can be a source of valuable information for public health in the concept of “One Health”. Full article

Conventional forward bleed slots reduce the hypersonic inlet starting Mach number but suffer from excessive flow leakage after restart. This paper proposes a novel reverse bleed slot design method for curved axisymmetric inlets of a solid-fuel scramjet. Leveraging the Kantrowitz criterion and detailed flow analysis, the method optimizes bleed slot placement, number, area, and angle. Results show superior aerodynamic performance by placing slots in the non-starting region of the internal compression section, considering both unstarted flow and separation bubble dynamics during restart. Each bleed slot area is calculated successively down-stream based on the Kantrowitz criterion. Finally, the effects of bleed slot angle have been extensively studied. The key inlet performance reaches its optimum at a slot angle of approximately 130°, achieving a significant reduction in the starting Mach number (from 4.80 to 3.65) and a 50% decrease in bleed flow rate compared to the forward slot design. This method demonstrates its feasibility and effectiveness, enabling substantial improvement in inlet starting performance with minimal flow loss. Full article

This study analyzed workload variations (internal, external, and mental) in training sessions based on soccer players’ match participation. Thirty-six professional Brazilian players from a single team were categorized into three groups: starter (G1), non-starter (G2) and non-participant (G3) players. Monitoring during sessions involved assessing internal load via perceived exertion, mental load through affective, emotional, and motivational factors, and external load using Global Positioning System devices. On MD+2, starter players exhibited significantly lower values in all categories compared to non-starters (p < 0.001) and non-participants (p < 0.001), while on MD, they displayed the highest values. MD-4 and MD-3 showed no cognitive or emotional load differences; however, variations were observed in RPE, motivation, mental fatigue, and physical metrics. Notably, starters’ lowest values occurred on MD-2 and MD-1. Findings emphasize the need to manage starter players’ load, implementing recovery strategies for optimal performance. Additionally, tailored tasks should be provided for non-starter and non-participant players to compensate for non-performed efforts during match downtime. This study underscores the significance of an individualized training approach based on players’ match participation, contributing valuable insights for optimizing performance and recovery strategies. Full article

Nonstarter lactic acid bacteria (NSLAB) are major contributors to the unique characteristics (e.g., aroma, flavor, texture) of dairy and nondairy fermented products. Lc. paracasei SRX10 is an NSLAB strain originally isolated from a traditional Greek cheese and previously shown to exhibit favorable biotechnological characteristics. More specifically, the strain showed tolerance to simulated gastrointestinal conditions, exopolysaccharide (EPS) biosynthetic capacity, and lack of hemolytic activity and was used in the production of yoghurt and feta cheese with distinct organoleptic characteristics. The aim of the present study was to investigate these traits at the genome level through whole-genome sequencing (WGS), annotation, and comparative genomics. Functional annotation of the genome revealed that Lc. paracasei SRX10 can utilize different carbon sources, leading to the generation of flavor compounds, including lactic acid, acetate, ethanol, and acetoin. Similarly, full clusters for fatty acid biosynthesis, protein and peptide degradation, as well as genes related to survival under extreme temperatures, osmotic shock, and oxidative stress were annotated. Importantly, no transferable antibiotic resistance genes or virulence factors were identified. Finally, strain-specific primers based on genome-wide polymorphisms were designed for the efficient and rapid identification of Lc. paracasei SRX10 via multiplex PCR in fermented products. Full article

Background and Objectives: Research on female soccer players that analyzes playing status is scarce and has previously only examined load monitoring, while other markers, such as physical (i.e., strength, power, and agility), physiological (i.e., maximal oxygen uptake), and body composition (i.e., body fat mass, fat-free mass, body water, and phase angle) markers, warrant further investigation. Thus, the study aims were to (a) compare physical, physiological, body composition, and load markers between starters and non-starters; (b) compare measurements pre- and post-training intervention (five weeks); and (c) analyze any relationships between physical, physiological, body composition, and load markers in an elite female soccer team. Materials and Methods: Fourteen first-team players participated in the study (age 23.29 ± 3.19 years, weight 59.14 ± 6.87 kg, height 1.66 ± 0.08 m). Several physical (n = 15), physiological (n = 1), body composition (n = 11), and load markers (n = 14) were collected. In addition, participants were sub-divided into starters (n = 7) and non-starters (n = 7). Results: No differences were revealed between starters and non-starters in any of the examined variables. Moreover, following the training intervention, a significantly lower value was found for total body water/fat-free mass ratio (p = 0.043; ES = 0.582). In addition, there were several correlations detected between load and physical/physiological markers (n = 28); load and body composition markers (n = 6); physical/physiological and body composition markers (n = 34); and physical and physiological markers (n = 42). Conclusions: In conclusion, only a slight tendency of higher load values for starters than non-starters was observed. In addition, no differences in physical, physiological, and body composition markers were found between starters and non-starters, possibly suggesting that five weeks were not enough to improve such variables. Finally, the present results provide novel information assessing the effects of the pre-season in elite female Portuguese soccer players and contribute to a better understanding of the associations between different types of measurements. Full article

This mini review deals with some controversial non-starter lactic acid bacteria (NSLAB) species known to be both human and animal pathogens but also health-promoting and probiotic. The focus is on Lactococcus garvieae, two Streptococcus species (S. uberis and S. parauberis), four Weissella species (W. hellenica, W. confusa, W. paramesenteroides, and W. cibaria), and Mammalicoccus sciuri, which worldwide, are often found within the microbiotas of different kinds of cheese, mainly traditional artisanal cheeses made from raw milk and/or relying on environmental bacteria for their ripening. Based on literature data, the virulence and health-promoting effects of these bacteria are examined, and some of the mechanisms of these actions are reviewed. Additionally, their possible roles in cheese ripening are also discussed. The analysis of the literature data available so far showed that, in general, the pathogenic and the beneficial strains, despite belonging to the same species, show somewhat different genetic constitutions. Yet, when the safety of a given strain is assessed, genomic analysis on its own is not enough, and a polyphasic approach including additional physiological and functional tests is needed. Full article

The microbial ecology fundamentals of raw milk and long-ripened cheeses consist of a complex interaction between starter lactic acid bacteria (SLAB) and non-starter LAB (NSLAB). Although NSLAB aromatic properties are paramount, other phenotypic traits need to be considered for their use as adjunct cultures, such as the capability to endure technological parameters encountered during cheesemaking. The present study focused on the isolation and characterization of NSLAB from spontaneously fermented raw cow’s milk coming from 20 dairies that produce Grana Padano PDO cheese. From 122 isolates, the screening process selected the 10 most diverse strains belonging to Lacticaseibacillus spp. to be phenotypically characterized. The strains were tested for their growth performance in milk in combination with the application of technological stresses, for their ability to produce volatile compounds after their growth in milk, and for their ability to use different nutrient sources and resist chemicals. The complex characterization qualified the strains 5959_Lbparacasei and 5296_Lbparacasei as the best candidates to be used as adjunct strains in the production of raw milk and long-ripened cheeses, provided that antibiotic resistance is measured before their employment. Other strains with interesting aromatic capabilities but lower heat resistance were 5293_Lbparacasei, 5649_Lbparacasei and 5780_Lbparacasei, which could be candidates as adjunct strains for uncooked cheese production. Full article