Search Results (144)

Constipation is a prevalent gastrointestinal disorder that significantly impairs quality of life. While pharmacological agents such as loperamide are widely used to induce constipation in experimental models, there is increasing interest in natural alternatives for alleviating intestinal dysfunction. In this study, we investigated the laxative effects of soybean powder fermented by Bacillus subtilis DKU_09 in a loperamide-induced rat model of constipation. The probiotic strain was isolated from cheonggukjang, a traditional Korean fermented soybean paste, and its identity was confirmed through 16S rRNA sequencing. Fermented soybean powder was characterized morphologically via scanning electron microscopy and chemically via HPLC to assess its isoflavone content. Rats were administered loperamide (5 mg/kg) for four days to induce constipation and were then treated with fermented soybean powder at doses of 100, 200, or 300 mg/kg. No pharmacological laxatives (e.g., PEG) were used as a positive control; instead, values from the treatment groups were compared with those from the loperamide-only constipation group. Key outcomes of fecal output, water content, colonic fecal retention, and gastrointestinal transit ratio were measured. The fermented product significantly improved stool frequency and moisture content, reduced colonic fecal retention, and restored gastrointestinal transit in a dose-dependent manner. Notably, the 300 mg/kg group demonstrated nearly complete recovery of fecal parameters without affecting body weight. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. These findings suggest that Bacillus subtilis-fermented soybean powder exerts synergistic laxative effects through the combined action of probiotic viability and fermentation-enhanced bioactive compounds such as aglycone isoflavones. This study supports the potential use of fermented soybean-based nutraceuticals as a natural and safe intervention for constipation and gastrointestinal dysregulation. Full article

Typo-morphology, as interpreted by the Italian School of Planning, provides an approach to investigate the relationship between built form and socio-cultural patterns in vernacular settlements. This study examines Harran, a heritage site in southeastern Türkiye known for its distinctive conic domed dwellings, to explore how typo-morphological analysis can inform culturally sensitive design and adaptive reuse approaches. Despite its historical significance and inclusion in the UNESCO tentative list, Harran faces insufficient documentation, fragmented governance, limited conservation, and increasing pressure from urbanization and natural disasters. Using multiple sources and fieldwork, the research reconstructs the morphological evolution of Harran through diachronic maps across compound, district, and town scales. Reflections from Alberobello, Italy, i.e., the sister city of Harran and a UNESCO-listed town with a similarly unique vernacular fabric, provide a comparative view to explore different heritage management approaches. Harran evolved through informal, culture-driven growth, whereas Alberobello followed a regulated path. While Alberobello benefits from planned development and institutional preservation, Harran faces partial abandonment and neglect. By positioning typo-morphology as a conceptual planning tool, this paper emphasizes the need for context-responsive, ethically grounded, and inclusive approaches to heritage planning and conservation. It argues for planning practices that are not only technically competent but also attuned to place-based knowledge, local identities, and the long-term sustainability of living heritage. Full article

Despite decades of ongoing efforts to address gender equality, female physicians, particularly women of color, continue to face significant barriers in medicine, which are exacerbated by microaggressions. In this qualitative study, 133 female physicians recalled experiences with microaggressions and the impact of these experiences on their professional identities and career progression. Thematic analysis revealed four major themes: (1) disregard for professional status; (2) undermining contributions; (3) intersectionality; (4) impact on career mobility and professional confidence. Further, there was one emerging theme: leadership and culture. The results underscore the compounding effects of microaggressions for female physicians with intersecting identities. Highlighting the application of social identity theory, intersectionality, and organizational justice, this study provides a comprehensive view of the impact of microaggressions on female physicians, offering new perspectives on the intersectional nature of discrimination and its effects on professional identity and career satisfaction. Full article

The objective of this work was to produce and characterize active gelatin–acerola packaging films based on gelatin incorporated with different concentrations of acerola pulp and applied to evaluate the stability of meat products in packaging. The active films were produced by casting using gelatin (5%), sorbitol (0,1%), and acerola pulp (60, 70, 80, and 90%). The characterization of the acerola pulp was carried out. Visual aspects, thickness, pH, water vapor permeability, and total phenolic compounds were characterized in the films. The commercial acerola pulp presented the characteristics within the identity and quality standards. A good film formation capacity was obtained in all formulations, presenting the color parameters tending to red coloration, characteristic of the acerola pulp. The total phenolic compounds content ranged from 2.88 ± 70.24 to 3.94 ± 96.05 mg GAE/100 g, with 90 g of acerola pulp per 100 g of filmogenic solution. This film formulation was selected to apply in a vacuum pack of beef and chicken samples, analyzing the weight loss, color parameters, pH, water holding capacity, shear strength after 9 days of refrigeration storage, and soil biodegradability. Additionally, beef and chicken (in nature) were stored under the same conditions without using the wrapping film. The beef and chicken samples showed greater water retention capacity and color maintenance over the storage period compared to the control (without film addition). This way, active gelatin–acerola films can be considered a sustainable packaging alternative to preserve meat products. Full article

Carob pulp powder (CPP), a by-product of Ceratonia siliqua L., is rich in bioactive compounds with potential for functional foods. This study evaluated how genetic variability among cultivars affects the nutritional composition and functional quality of CPP. Nineteen cultivars from Majorca (13), Ibiza (4), and two open-flowering selections were grown under identical conditions in Majorca, Spain. CPP samples showed high dietary fibre (22.6–47.4 g/100 g dry matter), total sugars (22.5–62.5 g/100 g dm), and antioxidant activity (11.1–78.4 µmol TE/g dm, CUPRAC method). Significant differences among cultivars were observed in protein and fructose content, fatty acid profiles, antioxidant activity, colour, acidity, and functional properties like water- and oil-holding capacities. Principal component analysis distinguished the Ibiza cultivars by higher soluble solids, fructose, and stearic acid content but lower antioxidant activity. Open-flowering selections exhibited the highest antioxidant and water retention capacities. These results confirm that genetic origin strongly influences CPP’s nutritional and functional traits, endorsing its use as a natural, health-promoting ingredient for functional food development. Full article

This paper presents field experiments of mineral deposition on steel, induced by cathodic polarization in natural seawater, as a sustainable strategy for the life extension of marine steel structures. Although this approach is quite well known, the ability of the mineral deposit to both protect steel from corrosion in the absence of a cathodic current, thus operating as an inorganic coating, and provide an effective substrate for colonization by microorganisms still needs to be fully explained. To this end, two identical steel structure prototypes were installed at a depth of 20 m: one was submitted to cathodic polarization, while the other was left under free corrosion for comparison. After 6 months, the current supplied to the electrified structure was interrupted. A multidisciplinary approach was used to analyze the deposits on steel round bars installed in the prototypes over time, in the presence and in the absence of a cathodic current. Different investigation techniques were employed to provide the following information on the deposit: the composition in terms of elements, compounds and macro-biofouling; the morphology; the thickness and the degree of protection estimated by electrochemical impedance spectroscopy (EIS). The results showed that under cathodic polarization, the thickness of the deposit increased to 2.5 mm and then remained almost constant after the current was interrupted. Conversely, the surface impedance decreased from 3 kΩ cm² to about 1.5 kΩ cm² at the same time, and the aragonite–brucite ratio also decreased. This indicates a deterioration in the protection performance and soundness of the deposit, respectively. Considering the trends in thickness and impedance together, it can be concluded that the preformed mineral deposit does not undergo generalized deterioration after current interruption, which would result in a reduction in thickness, but rather localized degradation. This phenomenon was attributed to the burrowing action of marine organisms, which created porosities and/or capillary pathways through the deposit. Therefore, the corrosion protection offered by the mineral deposit without a cathodic current is insufficient because it loses its protective properties. However, the necessary current can be quite limited in the presence of the deposit, which in any case provides a suitable substrate for sustaining the colonization and growth of sessile marine organisms, thus promoting biodiversity. Full article

Cinnamomum camphora chvar. Borneol essential oil (BEO, 16.4% borneol) is a by-product obtained during the steam distillation process used to produce natural crystalline borneol (NCB, 98.4% purity). This study aimed to compare the antibacterial activity of BEO and NCB against Staphylococcus epidermidis, and to evaluate the anti-inflammatory effect of BEO in vitro. Minimum inhibitory concentrations (MICs), determined by broth microdilution, were identical for both BEO and NCB (0.5 mg/mL). Despite this, BEO exhibited stronger antibacterial activity, suggesting synergistic enhancement by other components. Mechanistic studies revealed that BEO disrupted the bacterial cell wall, causing leakage of nucleic acids and proteins, and ultimately bacterial death. In LPS-induced RAW 264.7 macrophages, BEO dose-dependently reduced the production of TNF-α, IL-1β, and IL-6 (r = −0.9847, −0.9456, −0.9315). Network pharmacology, combined with primary and secondary factor analysis, was employed to identify anti-inflammatory pathways and key active compounds. Borneol contributed over 50% to the anti-inflammatory effect, followed by β-caryophyllene, limonene, camphor, and γ-terpinene. These findings highlight the potential enhanced bioactivity of BEO due to multi-component synergy. Full article

Antarctic microorganisms have genomic characteristics and biological functions to ensure survival in complex habitats, potentially representing bioactive compounds of biotechnological interest. Pseudarthrobacter sp. So.54 is an Antarctic bacteria strain isolated from the rhizospheric soil of Colobanthus quitensis. Our work aimed to study its genomic characteristics and metabolic potential, linked to environmental adaptation and the production of secondary metabolites with possible biotechnological applications. Whole-genome sequencing, assembly, phylogenetic analysis, functional annotation, and genomic islands prediction were performed to determine the taxonomic affiliation and differential characteristics of the strain So.54. Additionally, Biosynthetic Gene Clusters (BGCs) responsible for secondary metabolites production were identified. The assembled genome of strain So.54 has 3,871,805 bp with 66.0% G + C content. Phylogenetic analysis confirmed that strain So.54 belongs to the Pseudarthrobacter genus; nevertheless, its nucleotide and amino acid identity values were below the species threshold. The main metabolic pathways and 64 genomic islands associated with stress defense and environmental adaptation, such as heavy metal resistance genes, were identified. AntiSMASH analysis predicted six BGCs with low or no similarity to known clusters, suggesting potential as novel natural products. These findings indicate that strain So.54 could be a novel Pseudarthrobacter species with significant environmental adaptation and biotechnological potential. Full article

Pyrrole-type compounds are widely known for their potential biological activity. However, methods for synthesizing 2,3,4,5-tetrasubstituted pyrroles remain limited. This study explores an intramolecular cyclocondensation of 2-amino acid-derived enamines to yield novel 1-(5-substituted-4-hydroxy-2-methyl-1H-pyrrol-3-yl)ethan-1-ones. Using ʟ-alanine, ʟ-tyrosine, ʟ-phenylalanine, and ʟ-tryptophan, the corresponding 2-amino esters were synthesized, converted into enamines, and cyclized under microwave irradiation (55–86% yield). The highest yield was obtained from methyl ʟ-phenylalaninate (R¹ = CH₂Ph, R⁴ = Me). Steric hindrance from bulkier groups reduced yields, while the electronic nature of R¹ influenced reactivity. Structural analysis (NMR, HR-ESI-MS) confirmed product identities, and a 5-exo-trig cyclization mechanism explained base-mediated deprotonation and steric effects. These findings highlight steric and electronic factors in this cyclocondensation, guiding reaction optimization for valuable heterocycles. Full article

In recent decades, the scientific literature has increasingly linked specific human activities to various environmental issues, with climate change being a prominent concern. Environmental Psychology has thus placed its focus on comprehending the dynamics driving Pro-environmental Behaviors (PEBs). Research spotlights two crucial constructs fueling PEBs: Connectedness to Nature (CTN) and Environmental Attitudes (EAs). In the absence of a well-established model, this systematic review strives to advance the understanding of their compound relationship. Following PRISMA guidelines, a comprehensive literature search across Google Scholar, PsycInfo, PsycArticles, PubMed, Science Direct, Sociological Abstracts, and Academic Search Complete databases produced 2658 records. Of these records, only 19 studies met the eligibility criteria after passing the abstract selection phase and were consequently included in this systematic review. Our analysis revealed that CTN and EAs are distinct constructs but showed moderate-to-high correlations. This review posits two hypotheses: (i) CTN may function as a dimension of identity and serve as an EA antecedent and (ii) CTN could be a combination of both implicit and explicit EAs. Subsequently, an interaction model is devised to comprehensively elucidate PEB dynamics and guide effective interventions for their promotion in the context of sustainability. Full article

The physicochemical and sensory properties of wines are influenced by several factors, starting in the vineyard and evolving during the winemaking stages. After bottling, variables such as bottle position, closure type, storage temperature, and storage time shape wine characteristics. In this study, red wines stored for approximately 0.5 and 3 years with natural cork, micro-agglomerated cork stoppers, and screw cap closures were analyzed. Various techniques were employed to investigate changes during bottle storage, including the determination of volatile components by comprehensive gas chromatography-mass spectrometry with time-of-flight analyzer (GC × GC-ToFMS), phenolic profile by ultra-high-performance liquid chromatography, coupled with tandem mass spectrometry (UHPLC-DAD-MSⁿ), general physicochemical parameters, the oxygen transfer rate of cork stoppers, and sensorial analysis performed by a trained panel. The results revealed that the type of closure created distinct environments within the bottles, slightly influencing both sensory attributes and chemical evolution of the red wines. These findings highlight the value of combining diverse analytical techniques to reveal closure-driven differences, with volatile compound profiling emerging as the most sensitive methodology. Additionally, this study emphasizes that differences modulated by the wine–closure pairing, which become more pronounced during storage, can serve as an oenological tool in the construction of a wine’s identity. Full article

This study explores approaches to enhancing the biostability of extra virgin olive oil (EVOO) supplemented with olive fruit extract (OFE) enriched with hydroxytyrosol (HTyr). The investigation focuses on prolonged deep frying (DF) conditions at 170 °C and 210 °C, over durations ranging from 3 to 48 h, with the aim of improving sensorial attributes, polyphenolic content, and thermal oxidative stability. Parameters, such as acidity, peroxide value (PV), K₂₃₂, K₂₇₀, ΔK, phenolic compounds, and sensory attributes, were monitored. The PV did not exceed the standard limit in HTyr-EVOO at 210 °C/24 h; however, in non-supplemented EVOOs, it remained within the limits only up to 210 °C/18 h. Acidity stayed within the acceptable limit (≤0.8) at 170 °C/24 h in both enriched and non-enriched EVOOs. K₂₃₂ values were ≤2.5 in HTyr-EVOO fried at 170 °C/18 h. K₂₇₀ and ΔK did not exceed the limits in HTyr-EVOO at 170 °C/3 h, whereas they surpassed them in non-supplemented oils. Additionally, HTyr and tyrosol levels were significantly higher (p < 0.05) in HTyr-EVOOs. Phenolic compounds, including verbascoside, pinoresinol, 1-acetoxypinoresinol, and phenolic acids, such as chlorogenic, vanillic, homovanillic, 4-dihydroxybenzoic, and caffeic acids, were detected in HTyr-EVOOs. Oxidized secoiridoid derivatives increased significantly as DF progressed. Moreover, sensory analysis revealed that positive attributes in EVOOs—such as fruity, bitter, and pungent notes—decreased significantly with increasing temperature and frying duration (p < 0.05). Beyond 210 °C/6 h, these attributes were rated at zero. However, HTyr-EVOOs exhibited lower rancidity compared to non-enriched oils under identical conditions, attributed to the protective effect of HTyr. In conclusion, HTyr-EVOOs demonstrated thermal stability up to 210 °C/6 h, retaining desirable sensory qualities, higher phenolic content, and reduced degradation. These findings indicate that natural OFEs have strong potential as food additive in deep fried EVOOs, enhancing sensory properties, health benefits, and overall oil stability. This innovation provides a practical solution for the food industry by improving the biostability and versatility of EVOO. Further research is recommended to investigate various EVOO categories and oils from diverse origins. Full article

Breast cancer is the most common and the leading cause of cancer death in women worldwide; treating invasive breast carcinomas is challenging due to the side effects of chemotherapeutics. Compounds isolated from natural sources have been proposed as potential molecules for cancer therapy; for instance, the homoisoflavonoid brazilin has shown pharmacological properties, including anti-tumoral and anti-inflammatory activities. In this study, we isolated brazilin from the heartwood of Haematoxylum brasiletto; then, we performed a semi-synthesis by adding three methyl or acetyl groups to the core structure of brazilin. We confirmed the identity of brazilin and its derivatives by spectroscopic data (¹H NMR and ¹³C NMR) and measured their purity by optical rotation. Then, we analyzed the effects of brazilin and its derivatives in three mammary gland-derived cell lines: the TNBC MDA-MB-231, the ERα(+) MCF7, and the non-tumorigenic MCF10A. We evaluated the cell viability by MTT assays, cell migration by wound-healing assays, and focal adhesion kinase (FAK) activation by Western blot. Regarding biological assays, the MTT assay showed that these compounds showed cytotoxic effects on the MCF7 and MDA-MB-231 breast cancer cells at 20 µM but was not toxic in non-tumorigenic MCF10A mammary epithelial cells. Specifically, the greatest effects found from treatment with the compounds were in the MDA-MB-231 cell line, where the IC50 of brazilin was 49.92 μM, and for MCF7, the brazilin-(OAc)₃ was 49.97 μM. These effects were dose- and time-dependent, as well as being associated with a decrease in the levels of cell migration and FAK activation. Full article

Polyhydroxybutyrate (PHB) is a biodegradable natural polymer produced by different prokaryotes as a valuable carbon and energy storage compound. Its biosynthesis pathway requires the sole expression of the phaCAB operon, although auxiliary genes play a role in controlling polymer accumulation, degradation, granule formation and stabilization. Due to its biodegradability, PHB is currently regarded as a promising alternative to synthetic plastics for industrial/biotechnological applications. Azohydromonas lata strain H1 has been reported to accumulate PHB by using simple, inexpensive carbon sources. Here, we present the first de novo genome assembly of the A. lata strain H1. The genome assembly is over 7.7 Mb in size, including a circular megaplasmid of approximately 456 Kbp. In addition to the phaCAB operon, single genes ascribable to PhaC and PhaA functions and auxiliary genes were also detected. A comparative genomic analysis of the available genomes of the genus Azohydromonas revealed the presence of phaCAB and auxiliary genes in all Azohydromonas species investigated, suggesting that the PHB production is a common feature of the genus. Based on sequence identity, we also suggest A. australica as the closest species to which the phaCAB operon of the strain H1, reported in 1998, is similar. Full article

The oleoresin and volatile fraction produced by conifers, such as Pinus pinaster, play a crucial role in plant defence, acting as precursors to resin acids and adapting in response to environmental stress or pathogen attacks. Abietadiene (abieta-7,13-diene), the biosynthetic precursor to abietic acid, has been identified as the most abundant compound in extracts from pinewood nematode (PWN)-infected P. pinaster trees. As abietadiene is not commercially available, this study aimed to achieve, for the first time, its isolation, structure elucidation, and detailed characterization from readily available forestry residues. Abietadiene was successfully isolated using thin-layer chromatography (TLC), and its purity and identity were evaluated using multiple analytical techniques: gas chromatography (GC), liquid chromatography (LC), nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FTIR). GC analysis indicated a purity of over 70% for the isolated compound, while LC provided the higher purity value of 98%. The identity of abietadiene was unequivocally confirmed through LC, FTIR, and NMR analysis. This work represents the first isolation and comprehensive characterization of abietadiene from a natural source, making detailed chemical data on this compound available to the scientific community. These findings may be used for future studies on the biological interactions and ecological roles of abietadiene, particularly in the context of plant defence and pathogen resistance. Full article