Search Results (1,081)

The quality of edible oils is determined not only by the raw material, but also by the applied processing technology. Rapeseed oil is widely valued for its favorable fatty acid profile, however, the effects of combined pressing conditions and low-temperature bleaching on its lipid profile and calculated nutritional quality indices remain insufficiently characterized. This study aimed to evaluate the effects of oil production method and bleaching earth addition on the fatty acid profile, nutritional quality indices, and oxidizability-related indices of rapeseed oil from an applied food processing perspective. Rapeseed oil samples were obtained from a commercial producer using cold pressing, hot pressing, and mixed two-stage pressing. The oils were subjected to low-temperature bleaching with 1–5% bleaching earth. The fatty acid profile was determined by gas chromatography, and selected fatty acid-based indices were calculated and grouped into three categories: fatty acid class ratios, nutritional quality indices, and oxidizability-related indices. These included ratios describing the balance between omega-6 s omega-3 polyunsaturated fatty acids (Σ PUFA n-6/n-3), unsaturated and saturated fatty acids (Σ UFA/Σ SFA), and polyunsaturated and saturated fatty acids (Σ PUFA/Σ SFA). Health-oriented indices such as the Desirable Fatty Acids/Undesirable Fatty Acids ratio (Σ DFA/Σ OFA), hypocholesterolemic/hypercholesterolemic ratio (h/H), Nutritive Value Index (NVI), Atherogenic Index (AI), and Thrombogenic Index (TI), and oxidizability-related indices including the Unsaturation Index (UI), Peroxidizability Index (PI), Oxidizability Index (OI), calculated Oxidizability Value (Cox), and Oxidative Stability Index (OS). The results showed that the production method significantly influenced saturated fatty acid content and selected fatty acid-based nutritional quality indices. Cold-pressed oil was characterized by the lowest saturated fatty acid content and the most beneficial values of selected nutritional quality indices. Hot-pressed oil showed higher saturated fatty acid content and less favorable values for selected indices, whereas mixed oil generally showed values between those of cold-pressed and hot-pressed oils. However, differences in the main unsaturated fatty acid fractions were limited and, for most monounsaturated and polyunsaturated fatty acid parameters, were not statistically significant. Across all processing variants, the nutritional quality indices remained advantageous, with omega-6/omega-3 polyunsaturated fatty acid ratio values of 2.1–2.4, atherogenic index values of approximately 0.04–0.05, and thrombogenic index values of approximately 0.08–0.09. Although significant interactions between production method and bleaching earth addition were observed for selected oxidizability-related indices, the magnitude of bleaching-induced changes was limited. Overall, the findings indicate that low-temperature bleaching may be applied as a mild processing step without substantially compromising the fatty acid-based quality characteristics of rapeseed oil. The study provides practical insight into the relationship between pressing technology, bleaching conditions, and lipid quality assessment in industrial rapeseed oil production. Full article

Background/Objectives: Discrimination between iron deficiency anemia (IDA) and anemia of inflammation (AI), the major causes of anemia, remains a challenge and novel biomarkers are needed. This prospective study characterizes metabolomic changes in patients with anemia, compares IDA and AI, and evaluates the ability of metabolomic profiling to categorize otherwise unclassifiable anemia (UA) cases. Methods: In the single-center cross-sectional study, patients with anemia were classified as IDA, AI, or their combination (IDA + AI) according to traditional iron biomarkers. Targeted metabolomic analysis was conducted with tandem mass spectrometry using the MxP^® Quant 500 kit, with 617 individual metabolites and 227 calculated parameters in the final analysis. Results: The final cohort included 70 patients with anemia and 27 controls. The concentrations of six metabolites and six calculated parameters were significantly different in patients with anemia compared to controls. Anemia was associated with decreased concentrations of several polyunsaturated fatty acid (PUFA)-containing triglycerides (n = 3) and phosphatidylcholines (n = 2), as well as with increased asparagine/aspartate and docosahexaenoic acid/eicosapentaenoic acid ratio. Multivariate analysis using only metabolomic data differentiated between IDA and AI with a general classification accuracy of 88%. Unclassifiable anemia samples were reclassified mostly as IDA (n = 11) or IDA + AI (n = 6), with one sample reclassified as AI. Conclusions: Metabolomic markers of PUFAs differ between patients with anemia and controls, indicating possible alterations in lipid metabolism. Our preliminary findings suggest that metabolomic data may be capable of distinguishing between IDA and AI. However, owing to several limitations of the current study, the potential utility of metabolome-based approaches should be confirmed in independent validation studies. Full article

Background/Objectives: Dietary changes or increased physical activity to promote health accompanies breast cancer treatment to improve outcomes and prevent cancer recurrence. However, primary breast cancer prevention studies are limited and while there is a growing benefit to diet and exercise, the combinatory benefits of diet and exercise are limited. Therefore, the objective of this exploratory study was to determine the effects of exposure to diets high in n-3 or n-6 polyunsaturated fatty acids (PUFA) combined with exercise on mammary tumour development in female MMTV-neu(ndl)YD5 mice. Methods: Animals were fed maternal diets containing 10% safflower oil (n-6 PUFA-enriched) or 3% menhaden oil + 7% safflower oil (n-3 PUFA enriched) for 20 weeks (wks). Within diets, mice were further divided into sedentary control or exercise groups (n = 8–12). Exercised mice were run on a treadmill from 8 to 12 wks of age before tumours developed. Once tumours were detected, volume and multiplicity were measured throughout the study. Results: Mice consuming n-3 PUFA had reduced tumour volume by 55% (p < 0.001) and weight by 42% (p = 0.02) compared to mice fed n-6 PUFA. Exercised n-3 PUFA-fed mice experienced 10% longer tumour-free status (TD50) than their sedentary counterparts and 24% longer TD50 than sedentary mice fed the n-6 PUFA enriched diet (p = 0.001). Exercised n-6 PUFA-fed mice established tumours 11% sooner than their sedentary counterparts and developed the largest and greatest number of tumours throughout the study (p < 0.0001). Conclusions: Overall, diet quality plays a significant role in mammary tumour outcomes. The effect of exercise on tumour outcomes was not as clear, but this exploratory study highlights the need to consider the temporal trajectory of tumour development that impacts not only lifespan but healthspan. Full article

Production system may affect meat quality and muscle metabolic characteristics in Tibetan sheep. In this study, the biceps femoris muscles of twelve 3-year-old Tibetan sheep with similar body weights were used as experimental materials during a 6-month experimental period. The housed group (n = 6) was defined as the control group (C group), whereas the grazing group (n = 6) was defined as the L group. Meat quality measurement, nutritional composition analysis, untargeted lipidomics, and amino acid metabolomics (AAM) were integrated to investigate the effects of contrasting grazing and house-feeding production systems on meat quality and metabolic characteristics in Tibetan sheep. The results showed that cooking loss and drip loss were significantly decreased, whereas water-holding capacity (WHC) was significantly increased in the L group. However, shear force was also increased, indicating that grazing and house-feeding systems were associated with differences in muscle WHC and shear force. The L group exhibited significant alterations in lipid composition and increased concentrations of several n-3 polyunsaturated fatty acids and increased levels of omega-3 polyunsaturated fatty acids (n-3 PUFAs), including α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), suggesting that grazing and house-feeding systems were associated with differences in the lipid nutritional profile of muscle. Lipidomic analysis showed that the differential lipids were mainly enriched in triacylglycerols (TGs), phosphatidylethanolamines (PEs), and phosphatidylcholines (PCs), and several PUFA-containing TGs and membrane lipid molecules were closely associated with meat quality traits. AAM analysis showed that branched-chain amino acids (BCAAs), including L-leucine and L-valine, as well as N,N-dimethylglycine, were upregulated in the L group, whereas kynurenine and 1-methyl-L-histidine were downregulated. These findings suggest that BCAA metabolism and tryptophan–kynurenine metabolism were associated with metabolic differences observed between production systems in muscle metabolic adaptation. However, amino acid metabolomics analysis revealed that no amino acid metabolites remained significant after FDR correction, and thus the observed pathway-level changes (e.g., BCAA metabolism and tryptophan–kynurenine pathway) should be interpreted as nominal and exploratory findings. Overall, the results indicate that feeding systems were associated with alterations in the lipid and amino acid metabolic profiles of the biceps femoris muscle in Tibetan sheep, which were further associated with differences in muscle WHC, shear force, lipid nutritional composition, and the profile of flavor precursors. This study provides a theoretical basis for optimizing plateau meat sheep production systems and developing high-quality Tibetan sheep meat products. Full article

Enriching meat with n-3 polyunsaturated fatty acids (n-3 PUFAs) is of considerable nutritional interest because of the well-documented cardioprotective and anti-inflammatory properties of these fatty acids in the human diet. This study investigated the potential effects of dietary Artemisia ordosica Krasch (ARI) supplementation on muscle n-3 PUFA deposition in Albas White Cashmere goats, possibly mediated through the regulation of antioxidant capacity, lipid oxidation, and metabolism. Under the present experimental conditions, ARI supplementation did not significantly affect growth performance, but significantly reduced ruminal C18:0 content (p < 0.05) and increased n-3 PUFA levels (C18:3n3, C22:6n3) in rumen fluid, plasma, liver, and Longissimus dorsi, and the consequent elevation of the muscle PUFA-to-saturated fatty acid ratio (P/S; p < 0.05). Concurrently, ARI supplementation enhanced ruminal and systemic antioxidant capacity, as evidenced by increased glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities, elevated total antioxidant capacity (T-AOC), and upregulated expression of antioxidant-related genes (GSR, GOR, SOD2). Integrated multi-omics analyses revealed that these improvements may be associated with the reduced relative abundance of the core biohydrogenating bacterium s_Butyrivibrio_fibrisolvens and de novo saturated fatty acid synthesis, and potential involvement of the AMPK signaling pathway, with downregulation of FASN; coordinated upregulation of CD36, ACSL3/4, and ELOVL6/7; and upregulation of PPARGC1A, collectively suggesting a promotion of FA oxidation and n-3 PUFA deposition. Collectively, these findings suggest that ARI-promoted n-3 PUFA enrichment in goat meat may be associated with the coordinated modulation of ruminal biohydrogenation, systemic antioxidant capacity, and intermediary lipid metabolism, providing a potential nutritional strategy for developing functional cashmere goat meat. Full article

Background/Objectives: The obesity epidemic coexists with the phenomenon of “hidden hunger” (Type B malnutrition)—a micronutrient deficiency amidst a caloric excess. Traditional dietary assessment methods often distort the actual picture by ignoring technological losses during cooking, which necessitates the use of digital tools. Methods: A cross-sectional study (N = 3267) was conducted using the digital platform “NIAP”. The analysis was based on valid 3–7-day dietary records with algorithmic accounting for nutrient retention factors during thermal processing. The nutrient profiles of individuals with a normal body mass index (BMI) and obesity (BMI ≥ 30 kg/m²) were compared. Results: The epidemiology of intake shortfalls was highly prevalent and pronounced: 99.9% of the cohort had ≥1 inadequacy (with a mean negative deviation of −77.3% for vitamin D and −59.2% for Omega-3), and 61.5% exhibited ≥10 simultaneous multiple intake shortfalls. These inadequacy rates remained robust in a sensitivity analysis excluding under-reporters. The obesity group consumed significantly more energy, saturated fatty acids, added sugars, cholesterol, and sodium, but demonstrated a lower relative macronutrient intake (g/kg of body weight). Absolute fiber intake did not differ between the groups, indicating a decrease in its density per 1000 kcal in the diet of individuals with obesity; the intake of Omega-3 polyunsaturated fatty acids (PUFAs) showed a downward trend. The Na:K ratio was significantly higher in the obesity group (1.19 vs. 1.04, p < 0.001). Correlation analysis confirmed an inverse relationship between BMI and the overall nutrient density of the diet. Conclusions: A high-energy diet does not compensate for systemic micronutrient inadequacy among the evaluated cohort. Obesity is associated with a dietary imbalance favoring “empty calories” and pro-inflammatory components against a background of severe multiple dietary inadequacies. The integration of algorithmic dietary assessment that accounts for cooking losses is critical for objective diagnosis and personalized nutritional intervention. Full article

The aim of the current study is to determine the nutritional value and the content of the biologically active components in kidney vetch (Anthyllis vulneraria L.). It is established that the dry biomass contains substantial amounts of proteins and carbohydrates, primarily dietary fiber, while the total oil content is relatively low (below 3.0%). The isolated glyceride oil represents the complete lipid fraction derived from all plant parts (leaves, stems, and flowers). The glyceride oil of A. vulneraria is notable for its high levels of biologically active constituents, particularly sterols, tocopherols, and phospholipids. Palmitic (30.3%) and oleic (11.5%) acids dominate the fatty acid profile; β-sitosterol, α-tocotrienol, and α-tocopherol are the major sterol and tocopherol components, respectively. On the other hand, phosphatidylinositol, together with phosphatidic acids, prevails within the phospholipid fraction. Based on the obtained fatty acid composition, several important ratios were calculated—unsaturated fatty acids (UFA)/saturated fatty acids (SFA), saturated fatty acids/monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA)/saturated fatty acids, and n-6/n-3, providing an integrated assessment of the lipid quality. The PUFA/SFA value (0.24) suggests relatively high oxidative stability. In contrast, the n-6/n-3 ratio (0.86) shows a balanced distribution of essential fatty acids, which is associated with favorable nutritional properties. Full article

This study evaluated the effects of dietary supplementation with nano- and micro-encapsulated polyphenol extract (PE) from olive leaves (OL) and olive mill wastewater (OMWW), respectively, on growth, carcass and meat quality traits in Podolian young bulls. Fifteen 12-month-old bulls were assigned to three groups: C (control); T1 (40 g/day nano-encapsulated PE from OL); and T2 (400 g/day olive leaf pellets plus 30 g/day micro-encapsulated PE from OMWW) for 40 days. Final body weight and carcass yield were unaffected, although the average daily gain was higher in T2 (p < 0.05). Meat from T2 exhibited lower moisture and higher protein content (p < 0.01) compared with the other groups. T1 showed higher α-tocopherol levels (p < 0.05). Lipid oxidation was reduced in both treated groups (p < 0.01). Monounsaturated fatty acids tended to decrease in treated groups (p = 0.057), while saturated and polyunsaturated fatty acids (PUFA) were unaffected. However, T2 showed higher total n-3 PUFA (p < 0.05), and a more favourable n-6/n-3 ratio (p < 0.01) was found in treated groups. These results highlight the potential of olive–derived polyphenols as functional feed ingredients to enhance meat quality and promote sustainable, circular livestock systems. Full article

Sexual maturity in tiger pufferfish (Takifugu rubripes) consumes substantial metabolic energy, constraining somatic growth and limiting meat yield. Artificial triploid induction (sterility) may redirect energy toward somatic growth. Cold-shock induced triploids were compared with diploid controls using muscle and liver tissues, and the phenotype, histology, nutritional composition, hormone levels, as well as transcriptome profiles were assessed. After 5 months, triploids attained significantly greater body length and body weight, with larger muscle fiber diameter but lower fiber density. The triploids yielded lower ash content and higher amounts of total (TAA), essential (EAA), non-essential (NEAA), and delicious amino acids (DAA), as well as higher total fatty acids (TFA), total polyunsaturated fatty acids (PUFA), and omega 3 polyunsaturated fatty acids (n-3 PUFA). Growth-related hormones were elevated, including growth hormone (GH), testosterone (T), triiodothyronine (T₃), and thyroxine (T₄). Muscle transcriptome sequencing identified 231 differentially expressed genes, predominantly enriched in pathways related to cell growth regulation, metabolic processes, and energy conversion. These results provide reference data for breeding programs. Full article

Background: Gestational diabetes mellitus (GDM) adversely affects offspring neurobehavioral outcomes, yet evidence regarding continuous markers of maternal glucose metabolism remains limited. Although polyunsaturated fatty acids (PUFAs) have been shown to affect associations between glucose metabolism and respiratory outcomes, their effects on children’s emotional and behavioral problems remain unclear. This study investigated the association between maternal glucose metabolism and emotional and behavioral problems in children and the potential modifying effect of maternal erythrocyte PUFAs. Methods: This prospective birth cohort included 481 mother–child pairs. Maternal glucose metabolism during pregnancy was assessed using GDM diagnosis via a 75 g oral glucose tolerance test (OGTT), fasting plasma glucose (FPG), 1 h and 2 h OGTT glucose, hemoglobin A1c (HbA1c), insulin, and insulin resistance (HOMA-IR). Maternal erythrocyte PUFAs were quantified by gas chromatography. Children’s emotional and behavioral problems at age 5 years were assessed using the Strengths and Difficulties Questionnaire (SDQ). Generalized linear models were used to evaluate associations, including multiplicative interaction terms between glucose metabolism indicators and PUFAs. Results: Maternal FPG (OR = 1.63; 95%CI: 1.08–2.47), OGTT-1h glucose (OR = 1.84; 95%CI: 1.08–3.12), and HOMA-IR (OR = 1.52; 95%CI: 1.01–2.27) were each positively associated with an increased risk of abnormal total difficulties scores in children. Maternal insulin levels were positively associated with abnormal total difficulties scores in girls (p for interaction < 0.05). Higher maternal n-3 PUFA levels and lower n-6 PUFA levels attenuated the risk of glucose metabolism-related emotional and behavioral problems in children. Conclusion: Maternal glucose metabolism was associated with increased risk of emotional and behavioral problems in children. PUFA biomarkers could modify glucose-related emotional and behavioral outcomes in children. Full article

The aim of this study was to evaluate the influence of altitudinal grassland systems on forage antioxidant properties and the nutritional composition of beef produced in Caraș-Severin County, Romania. We hypothesised that cattle raised at higher altitudes would produce beef with a superior nutritional profile, characterised by a more favourable lipid composition and enhanced antioxidant-related characteristics. Samples of fresh grass and hay were gathered from three representative areas: plain (Sacu, 154 m), hill (Văliug, 550 m), and mountain (Cozia, 1130 m). The beef samples were represented by two categories of commercially important muscles: Longissimus thoracis (loin) and Semimembranosus (topside), sourced from animals raised in each location. The proximate composition of forage samples indicated substantially higher levels of fatty acids, protein, and ash in mountain grasslands compared to lowland regions (p < 0.05). The total polyphenol content (TPC) and antioxidant activity (DPPH test) revealed a similar pattern, with the strongest antioxidant activity (lowest IC₅₀ value) recorded for Cozia hay (GHC) samples. The composition of beef was additionally influenced by the production area. Samples derived from mountainous regions exhibited elevated protein concentrations, moderate intramuscular fat levels, and enhanced mineral composition in comparison to samples from plain areas. Fatty acid analysis revealed that mountain-sourced beef had significantly reduced levels of saturated fatty acids (SFA) and elevated concentrations of monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), including the nutritionally beneficial n-3 fatty acids and conjugated linoleic acid (CLA). Principal component analysis distinctly classified beef samples based on production method, with mountain-origin samples indicating better lipid properties and enhanced antioxidant-related variables. The findings demonstrate that natural grasslands at higher altitudes may enhance both the bioactive quality of fodder and the nutritional value of beef. Mountain pasture systems are a sustainable approach for producing high-quality beef with enhanced lipid composition and increased market value. Full article

Background/Objectives: Chemosymbiotic bivalves play a fundamental role in deep-sea cold seep and hydrothermal vent ecosystems, where essential long-chain polyunsaturated fatty acids (LC-PUFAs) are scarce. Whether these bivalves retain the capacity for endogenous PUFA synthesis remains poorly understood. Here, we investigated the PUFA biosynthetic capacity of two dominant chemosymbiotic bivalves from the Haima cold seep—the mussel Gigantidas haimaensis and the clam Archivesica marissinica. Methods: Genome and transcriptome assembly revealed three fatty acid desaturase (Fad) genes per species, which phylogenetically clustered into Δ5 (GhFads1, GhFads2; AmFads1, AmFads2) and Δ6/8 (GhFads3, AmFads3) clades, with lineage-specific duplications within the Δ5 clade. Functional assays were performed in yeast to characterize the activity of these enzymes. Results:Both Fads3 enzymes (Δ6/8 clade) convert C20:3n-6 and C20:4n-3 into C20:4n-6 and C20:5n-3, respectively, exhibiting Δ8-desaturase activity. Notably, Δ5-clade isoforms showed divergent substrate specificities: GhFads2 and AmFads1 functioned as classical Δ5-desaturases on PUFA substrates, whereas GhFads1 and AmFads2 specifically desaturated the bacterial monounsaturated fatty acid (MUFA) C18:1n-7 to produce C18:2n PUFAs. Conclusions: Together, our results reveal that cold-seep bivalves retain endogenous LC-PUFA biosynthetic capacity and have evolved duplicated Δ5-desaturases with novel regioselectivity toward bacterial MUFAs, likely representing an adaptive membrane modification for survival under extreme deep-sea conditions. Full article

Blood lipid responses to diet vary substantially between individuals, limiting the effectiveness of uniform dietary recommendations, and genetic variation may contribute to this heterogeneity through gene–diet interactions. This systematic review and meta-analysis evaluated nutrigenetic interactions affecting blood lipid traits. Web of Science Core Collection and MEDLINE were searched in April 2026 to identify human studies testing interactions between dietary exposures—including macronutrient composition, fat quantity, fat type [polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA)], carbohydrate, and protein—and lipid-related genes. Interaction p-values were synthesized using a weighted Stouffer’s Z method with Benjamini–Hochberg false discovery rate correction. Twenty studies (n = 20), comprising approximately 9800 participants, met the inclusion criteria. The most consistent evidence was observed for CETP, APOE, and APOB, particularly in relation to broader macronutrient composition and fat-related exposures, while ABCA1 and APOA5 showed significant but more limited evidence. PUFA was the most consistent specific dietary exposure. In contrast, ABCG5, ABCG8, and CYP7A1 lacked sufficient data for meta-analysis, highlighting major gaps in the current literature. Overall, the findings support the view that lipid responses to diet are partly genotype-dependent, while also underscoring the need for larger, better harmonized studies to clarify and extend the current evidence base. Full article

Background: Patients with chronic kidney disease (CKD) exhibit reduced serum levels of n-3 polyunsaturated fatty acids (PUFA). Alterations in fatty acid profiles may contribute to increased cardiovascular risk and potentially accelerate CKD progression. Aim: The aim of the study was to assess whether fatty acid profiles measured a decade earlier predicted CKD progression and cardiovascular events. Additionally, the impact of dietary patterns at baseline was evaluated. Methods: The study comprised 77 patients with CKD whose serum fatty acid profiles had been assessed approximately a decade earlier. Follow-up data on CKD progression, cardiovascular events, and all-cause mortality were collected. Dietary habits were assessed using a food frequency questionnaire. The composite endpoint was defined as renal replacement therapy initiation or occurrence of stroke, myocardial infarction, or death. Results: Higher n-3 PUFA content was significantly associated with a lower risk of the composite endpoint in Cox regression analysis (HR = 0.63; 95% CI: 0.40–0.99; p = 0.044). Significant differences in event-free survival were observed in patients with higher n-3/n-6 PUFA ratios (log-rank test, χ² = 4.58, p = 0.032). Patients who experienced stroke or myocardial infarction had significantly higher levels of n-6 PUFA (32.85% vs. 29.94% Mann–Whitney U test, p = 0.037) and lower n-3/n-6 PUFA ratios (0.07 vs. 0.08, Mann–Whitney U test, p = 0.045). Eicosapentaenoic acid (EPA) content was significantly lower at baseline in patients who required renal replacement therapy during follow-up compared with those who did not experience this outcome (0.66% [0.48–0.82] vs. 0.88% [0.64–1.13], Mann–Whitney U test, p = 0.02). Conclusions: Lower serum n-3 PUFA levels were observed in patients who reached the composite endpoint during follow-up. A higher n-3/n-6 PUFA ratio showed a protective effect in survival analysis, and higher EPA content was associated with a lower risk of renal replacement therapy initiation. A more favorable fatty acid profile may be linked to improved cardiovascular and renal outcomes. Further studies are needed to clarify the role of fatty acid profiles in long-term outcomes among patients with CKD, in whom cardiovascular disease remains the leading cause of death. Full article

The integration of alternative feeding strategies and diversified genetic resources represents a key approach to improving the sustainability of poultry production systems. This study evaluated the effects of genotype and diet on carcass traits, meat quality, fatty acid profile, and sensory characteristics in a fast-growing genotype (Ross 308), two Italian local breeds (Bionda piemontese and Robusta maculata), and their crosses with a medium-growing strain (Sasso). A total of 441 chickens were allocated according to a factorial design including genotype, diet (standard vs. low-input), and sex. At genotype-specific commercial endpoints, 240 carcasses were analyzed for carcass traits and meat quality, and a subset (n = 120) was used for chemical composition, fatty acid profile, and sensory evaluation. Ross 308 showed the highest carcass weight and breast yield, but also the highest cooking losses and lipid oxidation. Compared with Ross 308, local breeds and crossbred chickens exhibited lower carcass performance but also lower “wet feathers” scores and higher polyunsaturated fatty acid (PUFA) and n-3 proportions. The low-input diet reduced carcass weight and breast yield, impaired some sensory attributes, and shifted fatty acid composition towards lower PUFA and n-3 contents and a higher n-6/n-3 ratio. Overall, crossbred genotypes showed intermediate carcass performance and some meat quality traits comparable to those of local breeds. Full article